6-fused heteroaryldihydropyrimidines for the treatment and prophylaxis of hepatitis B virus infection

Abstract

The invention provides novel compounds having the general formula: ##STR00001##
wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5, R.sup.6, X, Y, W and n are as described herein, compositions including the compounds and methods of using the compounds.

Claims

1. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula I to a patient in need thereof, ##STR00237## wherein: R.sup.1 is hydrogen, chloro, bromo or methyl; R.sup.2 is hydrogen or fluoro; R.sup.3 is hydrogen, chloro or fluoro; R.sup.4 is methyl or ethyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen, methyl-O-carbonyl or carboxymethyl; X is carbonyl or sulfonyl; Y is CH.sub.2, O, N(R.sup.7), wherein R.sup.7 is hydrogen, methyl, isopropyl, t-butyl, cyclopropyl, methyl-O-carbonylisopropyl, carboxyethyl, carboxypropyl, carboxybutyl, carboxy(gemdimethyl)methyl, carboxy(gemdimethyl)ethyl, carboxy(gemdimethyl)propyl, carboxy(gemdimethyl)butyl, carboxy(methyl)ethyl, carboxy(ethyl)ethyl, carboxy(methoxy) ethyl, carboxycyclobutyl, carboxycyclobutylmethyl, carboxycyclopentyl, carboxycyclohexyl, carboxymethylcyclopropyl, carboxycyclopropylmethyl, carboxycyclobutylmethyl, carboxyspiro[3.3]heptyl, carboxymethoxyethyl, carboxymethoxypropyl, hydroxyethyl, hydroxymethyl (gemdimethyl)butyl, carboxyphenyl or carboxyphenylmethyl; W is CH.sub.2, C(CH.sub.3).sub.2, Oor carbonyl; and n is 0 or 1; or pharmaceutically acceptable salts, or enantiomers thereof.

2. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (IA) to a patient in need thereof, ##STR00238## wherein: R.sup.1 is halogen or C1-6alkyl; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C1-6alkyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen, C1-6alkoxycarbonyl or carboxy-C.sub.mH.sub.2m; Y is N(R.sup.7), wherein R.sup.7 is hydrogen, C.sub.1-6alkyl, C.sub.3-7cycloalkyl, C.sub.1-6alkoxycarbonyl-C.sub.mH.sub.2m, C.sub.mH.sub.2mCOOH, (C.sub.1-6alkoxy) C.sub.1-6alkyl-COOH, C.sub.1-6alkyl-OC.sub.1-6alkyl-COOH, C.sub.3-7cycloalkyl-C.sub.mH.sub.2mCOOH, C.sub.mH.sub.2mC.sub.3-7cycloalkyl-COOH, hydroxy-C.sub.mH.sub.2m, carboxyspiro[3.3]heptyl or carboxyphenyl-C.sub.mH.sub.2m; W is CH.sub.2or carbonyl; and m is 0-7; or pharmaceutically acceptable salts, or enantiomers thereof.

3. A method according to claim 1, wherein: R.sup.1 is chloro, bromo or methyl; Y is N(R.sup.7), wherein R.sup.7 is hydrogen, methyl, isopropyl, t-butyl, cyclopropyl, methyl-O-carbonylisopropyl, carboxyethyl, carboxypropyl, carboxybutyl, carboxy(gemdimethyl)methyl, carboxy(gemdimethyl)ethyl, carboxy(gemdimethyl)propyl, carboxy(gemdimethyl)butyl, carboxy(methyl)ethyl, carboxy(ethyl)ethyl, carboxy(methoxy) ethyl, carboxycyclobutyl, carboxycyclobutylmethyl, carboxycyclopentyl, carboxycyclohexyl, carboxymethylcyclopropyl, carboxycyclopropylmethyl, carboxycyclobutylmethyl, carboxyspiro[3.3]heptyl, carboxymethoxyethyl, carboxymethoxypropyl, hydroxyethyl, hydroxymethyl (gemdimethyl)butyl, carboxyphenyl or carboxyphenylmethyl; and W is CH.sub.2or carbonyl.

4. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (IAA) to a patient in need thereof, ##STR00239## wherein: R.sup.1 is halogen or C.sub.1-6alkyl; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C.sub.1-6alkyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen; R.sup.7 is C.sub.1-6alkyl, C.sub.3-7cycloalkyl, C.sub.mH.sub.2mCOOH, C.sub.mH.sub.2mC.sub.3-7cycloalkyl-COOH or carboxyphenyl; and m is 1-6; or pharmaceutically acceptable salts, or enantiomers thereof.

5. A method according to claim 1, wherein: R.sup.1 is chloro or methyl; R.sup.3 is hydrogen or fluoro; R.sup.6 is hydrogen; and R.sup.7 is methyl, isopropyl, t-butyl, cyclopropyl, carboxy(gemdimethyl)ethyl, carboxy(gemdimethyl)propyl, carboxycyclopropylmethyl, carboxycyclobutylmethyl or carboxyphenyl.

6. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (IB) to a patient in need thereof, ##STR00240## wherein: R.sup.1 is hydrogen or halogen; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C.sub.1-6alkyl; R.sup.5 is hydrogen; R.sup.6 is hydrogen or carboxymethyl; Y is CH.sub.2or O; W is CH.sub.2, C(C.sub.1-6alkyl).sub.2- or O; and n is 0 or 1; or pharmaceutically acceptable salts, or enantiomers thereof.

7. A method according to claim 1, wherein: R.sup.1 is hydrogen, chloro or bromo; R.sup.3 is hydrogen or fluoro; R.sup.5 is hydrogen; R.sup.6 is hydrogen or carboxymethyl; Y is CH.sub.2or O; and W is CH.sub.2, C(CH.sub.3).sub.2or O.

8. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (ID) to a patient in need thereof, ##STR00241## wherein: R.sup.1 is halogen or C.sub.1-6alkyl; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C.sub.1-6alkyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen or C.sub.1-6alkoxycarbonyl X is carbonyl; Y is Oor N(R.sup.7), wherein R.sup.7 is hydrogen, C.sub.1-6alkyl, C.sub.3-7cycloalkyl, C.sub.mH.sub.2mCOOHC.sub.mH.sub.2mC.sub.3-7cycloalkyl-COOH, hydroxy-C.sub.mH.sub.2m, carboxyspiro[3.3]heptyl or carboxyphenyl-C.sub.tH.sub.2t; m is 1-6; and t is 0-6; or pharmaceutically acceptable salts, or enantiomers thereof.

9. A method according to claim 1, wherein: R.sup.1 is chloro, bromo or methyl; R.sup.3 is hydrogen or fluoro; R.sup.6 is hydrogen or methyl-O-carbonyl; X is carbonyl; and Y is O, or N(R.sup.7), wherein R.sup.7 is hydrogen, methyl, isopropyl, t-butyl, cyclopropyl, carboxy(gemdimethyl)ethyl, carboxy(methyl)ethyl, carboxycyclopropylmethyl, carboxyphenyl, carboxycyclopentyl, carboxycyclohexyl, carboxy(gemdimethyl)propyl, carboxy(gemdimethyl)butyl, carboxycyclobutylmethyl, carboxyspiro[3.3]heptyl, hydroxyethyl or carboxyphenylmethyl.

10. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (IE) to a patient in need thereof, ##STR00242## wherein: R.sup.1 is halogen or C.sub.1-6alkyl; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C.sub.1-6alkyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen or carboxy-C.sub.mH.sub.2m; Y is Oor N(R.sup.7), wherein R.sup.7 is C.sub.1-6alkyl, C.sub.3-7cycloalkyl, C.sub.mH.sub.2mCOOH, C.sub.3-7cycloalkyl-C.sub.mH.sub.2mCOOH, C.sub.mH.sub.2mC.sub.3-7cycloalkyl-COOH, (C.sub.1-6alkoxy) C.sub.1-6alkyl-COOH, C.sub.1-6alkyl-OC.sub.1-6alkyl-COOH, carboxyspiro[3.3]heptyl or carboxyphenyl-C.sub.mH.sub.2m; W is CH.sub.2or C(C.sub.1-6alkyl).sub.2-; and m is 0-6; or pharmaceutically acceptable salts, or enantiomers thereof.

11. A method according to claim 1, wherein: R.sup.1 is chloro or methyl; R.sup.3 is hydrogen or fluoro; R.sup.6 is hydrogen or carboxymethyl; Y is Oor N(R.sup.7), wherein R.sup.7 is isopropyl, methyl, t-butyl, cyclopropyl, carboxyethyl, carboxypropyl, carboxybutyl, carboxy(gemdimethyl)methyl, carboxy(gemdimethyl)ethyl, carboxy(methyl)ethyl, carboxycyclobutyl, carboxycyclopropylmethyl, carboxycyclopentyl, carboxycyclohexyl, carboxymethylcyclopropyl, carboxy(gemdimethyl)propyl, carboxy(ethyl)ethyl, carboxy(methoxy) ethyl, carboxycyclobutylmethyl, carboxyspiro[3.3]heptyl, carboxymethoxyethyl, carboxymethoxypropyl, carboxyphenylmethyl or carboxyphenyl; and W is CH.sub.2or C(CH.sub.3).sub.2.

12. A method of inhibiting HBV capsid, which method comprises administering an effective amount of a compound of formula (IE) to a patient in need thereof, ##STR00243## wherein: R.sup.1 is halogen or C.sub.1-6alkyl; R.sup.2 is hydrogen or halogen; R.sup.3 is hydrogen or halogen; R.sup.4 is C.sub.1-6alkyl; R.sup.5 is hydrogen or carboxy; R.sup.6 is hydrogen or carboxy-C.sub.mH.sub.2m; Y is N(R.sup.7), wherein R.sup.7 is hydrogen, C.sub.1-6alkyl, C.sub.3-7cycloalkyl, C.sub.mH.sub.2mCOOH, C.sub.mH.sub.2mC.sub.3-7cycloalkyl-COOH or carboxyphenyl; W is CH.sub.2; and m is 1-6; or pharmaceutically acceptable salts, or enantiomers thereof.

13. A method according to claim 2, wherein: R.sup.1 is chloro or methyl; R.sup.3 is hydrogen or fluoro; R.sup.6 is hydrogen or carboxymethyl; and Y is N(R.sup.7), wherein R.sup.7 is hydrogen, methyl, t-butyl, cyclopropyl, carboxy(gemdimethyl)ethyl, carboxy(gemdimethyl)propyl, carboxy(methyl)ethyl, carboxycyclopropylmethyl, carboxycyclobutylmethyl or carboxyphenyl.

Description

BRIEF DESCRIPTION OF THE FIGURE

(1) FIG. 1. X-ray crystal structure of Compound B1

EXAMPLES

(2) The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.

(3) Abbreviations used herein are as follows:

(4) []D.sub.20: optical rotation at 20 degrees Celsius BOMCl benzylchloromethyl ester calc'd: calculated CC.sub.50: concentration results in the death of 50 percent of the cells CCK-8: cell counting kit-8 CCl.sub.4: carbon tetrachloride Ct: cycle threshold d: day DIPEA: N,N-diisopropylethylamine DCM: dichloromethylene PE: petroleum ether DMSO: dimethylsulfoxide DEA: diethyl amine DNA: deoxyribonucleic acid EtOH: ethanol EtOAc or EA: ethyl acetate g: gram EC.sub.50: half maximal effective concentration h or hr: hour hrs: hours HAP: heteroaryldihydropyrimidine HATU: 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate HBeAb: hepatitis B e antibody HBeAg: hepatitis B e antigen HBsAg: hepatitis B surface antigen HCl: hydrogen chloride HPLC: high performance liquid chromatography HPLC-UV: high performance liquid chromatography with ultraviolet detector Hz: hertz IPA: isopropanol LDA: lithium diisopropylamide METHANOL-d.sub.4: deuterated methanol MeOH: methanol mg: milligram MHz: megahertz min: minute mins: minutes mL: milliliter mm: millimeter mM: mmol/L mmol: millimole MS: mass spectrometry MW: molecular weight Na.sub.2SO.sub.4: sodium sulfate NaOH: sodium hydroxide NBS: N-bromosuccinimide NMR: nuclear magnetic resonance PBS: phosphate buffered saline PD: pharmacodynamics PK: pharmacokinetics prep-HPLC: preparative high performance liquid chromatography rt: room temperature sat. saturated SFC: supercritical fluid chromatography TEA: triethylamine Tet: tetracycline TFA: trifluoroacetic acid THF: tetrahydrofuran g: microgram L: microliter M: micromole UV: ultraviolet detector OD: optical density pgRNA: pre-genomic RNA qPCR: quantitative polymerase chain reaction qRT-PCR: quantitative real-time polymerase chain reaction CYP: cytochromes P450 [Ir(COD).sub.2Cl].sub.2:bis(1,5-cyclooctadiene)diiridium(I) dichloride Cs.sub.2CO.sub.3: cesium carbonate Pd(OAc).sub.2: palladium(II) acetate NaBH.sub.4: sodium borohydride RuCl.sub.3: ruthenium(III) chloride
General Experimental Conditions

(5) Intermediates and final compounds were purified by flash chromatography using one of the following instruments: i) Biotage SP1 system and the Quad 12/25 Cartridge module. ii) ISCO combi-flash chromatography instrument. Silica gel brand and pore size: i) KP-SIL 60 , particle size: 40-60 M; ii) CAS registry NO: Silica Gel: 63231-67-4, particle size: 47-60 micron silica gel; iii) ZCX from Qingdao Haiyang Chemical Co., Ltd, pore: 200-300 or 300-400.

(6) Intermediates and final compounds were purified by preparative HPLC on reversed phase column using XBridge Prep-C18 (5 rim, OBD 30100 mm) column or SunFire Prep-C18 (5 m, OBD 30100 mm) column. Waters AutoP purification System (Column: XBridge Prep-C18, 30100 mm, Sample Manager 2767, Pump 2525, Detector: Micromass ZQ and UV 2487, solvent system: acetonitrile and 0.1% ammonium hydroxide in water). For SFC chiral separation, intermediates were separated by chiral column (Daicel chiralpak IC, 5 m, 30250 mm) column using Mettler Toledo SFC-Multigram III system, solvent system: 95% CO.sub.2 and 5% IPA (0.5% TEA in IPA), back pressure 100 bar, detection UV@ 254 nm.

(7) LC/MS spectra of compounds were obtained using a LC/MS (Waters Alliance 2795-Micromass ZQ), LC/MS conditions were as follows (running time 6 min):

(8) Acidic condition: A: 0.1% formic acid in H.sub.2O; B: 0.1% formic acid in acetonitrile;

(9) Basic condition: A: 0.1% NH.sub.3H.sub.2O in H.sub.2O; B: acetonitrile;

(10) Neutral condition: A: H.sub.2O; B: acetonitrile.

(11) Mass spectra (MS): generally only ions which indicate the parent mass are reported, and unless otherwise stated the mass ion quoted is the positive mass ion (MH).sup.+.

(12) NMR Spectra were obtained using Bruker Avance 400 MHz.

(13) A single crystal was mounted in a loop and cooled to 160 K in a nitrogen stream. Data were collected on a Gemini R Ultra diffractometer (Oxford Diffraction, UK) with CuK-alpha-radiation (1.54178 ) and processed with the Crysalis-package. Structure solution and refinement was performed using the ShelXTL software (Bruker AXS, Karlsruhe)

(14) The microwave assisted reactions were carried out in a Biotage Initiator Sixty microwave synthesizer.

(15) All reactions involving air-sensitive reagents were performed under an argon atmosphere. Reagents were used as received from commercial suppliers without further purification unless otherwise noted.

PREPARATIVE EXAMPLES

(16) The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.

Example 1

Methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(6-oxo-1,3,4,8,9,9a-hexahydropyrazino[1,2-c][1,3]oxazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(17) ##STR00029##

(18) The title compound was prepared according to the general synthetic routes shown in Scheme 1. A detailed synthetic route is provided in Scheme 2.

(19) ##STR00030##

Preparation of Compound A

(20) To a stirred solution of thiazole-2-carbonitrile (1.5 g, 14 mmol) in 5 mL of dry MeOH was added dropwise a solution of sodium methoxide (0.74 g, 14 mmol) in 10 mL of dry methanol. The reaction mixture was stirred at room temperature until the disappearance of starting material.

(21) After that, ammonium chloride (1.5 g, 28 mmol) was added in one portion and the reaction mixture was stirred overnight. The undissolved material was removed by filtration and the filtrate was concentrated to afford thiazole-2-carboxamidine hydrochloride (Compound A) as a grey solid which was used directly in the next step without further purification. MS: calc'd 128 (MH.sup.+), measured 128 (MH.sup.+).

Preparation of Compound B

(22) To a stirred solution of thiazole-2-carboxamidine hydrochloride (0.13 g, 1.0 mmol), methyl acetoacetate (0.12 g, 1.0 mmol) and 2-chloro-4-fluorobenzaldehyde (0.16 g, 1.0 mmol) in trifluoroethanol (8 mL) was added potassium acetate (0.20 g, 2.0 mmol). The reaction mixture was refluxed for 16 hours. After it was cooled to room temperature, the reaction mixture was concentrated and the residue was dissolved in ethyl acetate and then washed with brine. The organic layer was dried over sodium sulfate. The solvent was concentrated, and the residue was purified by column chromatography (ethyl acetate/petroleum ether is from to ) to afford 4-(2-chloro-4-fluoro-phenyl)-6-methyl-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound B) as a yellow solid. MS: calc'd (MH.sup.+) 366, measured (MH.sup.+) 366.

Preparation of Compound B1

(23) The enantiomer (R)-4-(2-chloro-4-fluoro-phenyl)-6-methyl-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound B1) was obtained through SFC (SFC-Multigram; IC: 5250 mm, 5) chiral separation of 4-(2-chloro-4-fluoro-phenyl)-6-methyl-2-oxazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound B) eluting with a mixed solvent of 85% supercritical CO.sub.2/15% EtOH at 100 mL/min rate. The desired ()-enantiomer B1 was eluted out before (+)-enantiomer B2. The absolute configuration of ()-enantiomer B1 was determined by X-ray diffraction study (FIG. 1).

(24) Compound B1: []D.sup.20 55.0 (c 0.845, MeOH).

(25) Compound B2: []D.sup.20+44.6 (c 0.175, MeOH).

Preparation of Compound C

(26) To a stirred solution of (R)-4-(2-chloro-4-fluoro-phenyl)-6-methyl-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (0.37 g, 1.0 mmol) in CCl.sub.4 (5 mL) was added NBS (0.20 g, 1.1 mmol) in portions. After the reaction mixture was stirred at room temperature for 1 hour, the solvent was removed in vacuo and the residue was purified by column chromatography to give (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) as a yellow solid. MS: calc'd 445 (MH.sup.+), measured 445 (MH.sup.+).

Preparation of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D)

(27) ##STR00031##
Step 1:

(28) Methyl 4-boc-piperazine-2-acetate (CAS number: 183742-33-8, Chemfinder) (1.0 g, 4 mmol) was dissolved in 10 mL of MeOH, then NaBH.sub.4 (1.5 g, 40 mmol) was added portion wise and stirred overnight. The solvent was removed in vacuo and the residue was partitioned between EtOAC and water. The organic layer was separated, and then dried over Na.sub.2SO.sub.4 and concentrated to give 3-(2-hydroxy-ethyl)-piperazine-1-carboxylic acid tert-butyl ester as crude oil (compound E), which was used directly without further purification.

(29) Step 2:

(30) 3-(2-Hydroxy-ethyl)-piperazine-1-carboxylic acid tert-butyl ester (58 mg, 0.25 mmol) was dissolved in 4 mL of dichloromethane and 1 mL of DIPEA, and then triphosgene (27 mg, 0.09 mmol) was added at room temperature. The reaction mixture was stirred for 30 minutes, and the solvent was removed in vacuo. The residue was dissolved in 3 mL of dichloromethane and 1 mL of TFA. After the reaction mixture was stirred for 1 hour, the solvent was removed in vacuo to give hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one trifluoro acetitic acid salt (Compound D) as a crude product which was used directly without further purification. LC/MS: calc'd 157 (MH.sup.+), exp 157 (MH.sup.+).

Preparation of Example 1

(31) To a stirred solution of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (0.072 g, 0.16 mmol) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one trifluoro acetitic acid salt (Compound D, crude, 0.25 mmol) in 1,2-dichloroethane (5 mL) was added dropwise DIPEA (0.078 mL, 0.45 mmol). The reaction mixture was stirred at room temperature until the disappearance of starting material. The mixture was diluted with EtOAc (50 mL) and washed successively with saturated aqueous NH.sub.4Cl solution and brine. The organic layer was separated and then dried over Na.sub.2SO.sub.4. The solvent was concentrated in vacuo and the crude product was purified by prep-HPLC to give methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(6-oxo-1,3,4,8,9,9a-hexahydropyrazino[1,2-c][1,3]oxazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Example 1) as a light yellow solid (11 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (dd, J=3.01, 1.00 Hz, 1H), 7.81 (d, J=3.01 Hz, 1H), 7.46 (dd, J=8.66, 6.15 Hz, 1H), 7.26 (dd, J=8.78, 2.51 Hz, 1H), 7.00-7.13 (m, 1H), 6.19 (d, J=1.76 Hz, 1H), 4.19-4.48 (m, 4H), 4.11 (d, J=16.06 Hz, 1H), 3.76-3.94 (m, 1H), 3.62 (s, 3H), 3.18-3.31 (m, 2H), 2.37-2.80 (m, 3H), 2.11-2.30 (m, 1H), 1.90 (qdd, J=14.37, 14.37, 14.37, 9.22, 5.40 Hz, 1H). MS: calc'd 520 (MH.sup.+), measured 520 (MH.sup.+).

Example 2

Methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(4-oxo-6,7,9,9a-tetrahydro-1H-pyrazino[2,1-c][1,4]oxazin-8-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(32) ##STR00032##

(33) The title compound was prepared in analogy to Example 1 by using 1,6,7,8,9,9a-hexahydropyrazino[2,1-c][1,4]oxazin-4-one (WuXi AppTec (Wuhan) Co., Ltd, catalog number: WX111240; for its synthesis, please refer to: Tang, Pengcho et al., PCT Int. Appl., 2012019426) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 2 was obtained as a light yellow solid (9 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.14 Hz, 1H), 7.76 (d, J=3.14 Hz, 1H), 7.43 (dd, J=8.72, 6.09 Hz, 1H), 7.24 (dd, J=8.72, 2.57 Hz, 1H), 6.96-7.12 (m, 1H), 6.18 (d, J=2.26 Hz, 1H), 4.46-4.66 (m, 1H), 4.05-4.22 (m, 3H), 3.77-4.05 (m, 3H), 3.53-3.73 (m, 4H), 2.94-3.13 (m, 2H), 2.81-2.94 (m, 1H), 2.15-2.52 (m, 2H). MS: calc'd 520 (MH.sup.+), measured 520 (MH.sup.+).

Example 3

Methyl (4R)-6-[[(8aR)-3-oxo-5,6,8,8a-tetrahydro-1H-oxazolo[3,4-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(34) ##STR00033##

(35) The title compound was prepared in analogy to Example 1 by using (S)-4-N-Boc-2-hydroxymethyl-piperazine (CAS number: 314741-40-7, Bepharm) instead of 3-(2-hydroxy-ethyl)-piperazine-1-carboxylic acid tert-butyl ester (Compound E). Example 3 was obtained as a light yellow solid (21 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.93-8.00 (m, 1H), 7.73-7.84 (m, 1H), 7.40-7.50 (m, 1H), 7.25 (dd, J=8.66, 2.64 Hz, 1H), 7.00-7.12 (m, 1H), 6.15-6.22 (m, 1H), 4.42-4.57 (m, 1H), 4.25 (d, J=16.81 Hz, 1H), 4.13-4.22 (m, 1H), 3.99-4.13 (m, 2H), 3.78-3.90 (m, 1H), 3.56-3.67 (m, 3H), 3.29-3.39 (m, 1H), 3.25 (d, J=9.03 Hz, 1H), 2.96 (d, J=11.04 Hz, 1H), 2.30-2.61 (m, 2H). MS: calc'd 506 (MH.sup.+), measured 506 (MH.sup.+).

Example 4

Methyl (4R)-6-[[(8aS)-3-oxo-5,6,8,8a-tetrahydro-1H-oxazolo[3,4-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(36) ##STR00034##

(37) The title compound was prepared in analogy to Example 1 by using (R)-4-N-boc-2-hydroxymethyl-piperazine (CAS number: 278788-66-2, Bepharm) instead of 3-(2-hydroxy-ethyl)-piperazine-1-carboxylic acid tert-butyl ester (Compound E). Example 4 was obtained as a light yellow solid (20 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.92-8.00 (m, 1H), 7.78 (d, J=3.26 Hz, 1H), 7.44 (dd, J=8.78, 6.02 Hz, 1H), 7.25 (dd, J=8.66, 2.64 Hz, 1H), 7.01-7.12 (m, 1H), 6.14-6.21 (m, 1H), 4.46 (t, J=8.53 Hz, 1H), 4.18-4.26 (m, 1H), 4.08-4.18 (m, 1H), 3.97-4.08 (m, 2H), 3.82-3.93 (m, 1H), 3.53-3.67 (m, 3H), 3.34-3.40 (m, 1H), 3.08 (d, J=10.54 Hz, 2H), 2.46-2.60 (m, 1H), 2.28-2.44 (m, 1H). MS: calc'd 506 (MH.sup.+), measured 506 (MH.sup.+).

Example 5

Methyl (4R)-6-[[(8aS)-6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(38) ##STR00035##

(39) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound F). Example 5 was obtained as a light yellow solid (14 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.90-7.99 (m, 1H), 7.72-7.82 (m, 1H), 7.36-7.52 (m, 1H), 7.20-7.31 (m, 1H), 7.06 (td, J=8.41, 2.76 Hz, 1H), 6.18 (s, 1H), 4.18 (d, J=17.07 Hz, 1H), 3.97-4.04 (m, 2H), 3.86-3.97 (m, 1H), 3.61 (s, 3H), 3.21 (d, J=9.29 Hz, 1H), 3.03-3.16 (m, 1H), 2.83-2.95 (m, 1H), 2.36-2.56 (m, 2H), 2.19-2.35 (m, 3H), 1.66-1.82 (m, 1H). MS: calc'd 504 (MH.sup.+), measured 504 (MH.sup.+).

(40) ##STR00036##

Preparation of Methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound F)

(41) Compound F was prepared in analogy to Example 1 by using hexahydropyrrolo[1,2-a]pyrazin-6(2H)-one (CAS number: 117810-52-3, Bepharm; for its synthesis, please refer to: Alvaro G., Large C. PCT Int. Appl., 2008090115) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D).

Example 6

Methyl (4R)-6-[[(8aR)-6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(42) ##STR00037##

(43) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound F) in Example 5. Example 6 was obtained as a light yellow solid (14 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.87-8.01 (m, 1H), 7.71-7.81 (m, 1H), 7.36-7.49 (m, 1H), 7.19-7.29 (m, 1H), 7.06 (td, J=8.41, 2.51 Hz, 1H), 6.18 (s, 1H), 4.11-4.21 (m, 1H), 4.06 (dd, J=12.55, 3.01 Hz, 1H), 3.95-4.02 (m, 1H), 3.89 (dtd, J=10.73, 7.25, 7.25, 3.64 Hz, 1H), 3.53-3.65 (m, 3H), 3.01-3.19 (m, 3H), 2.33-2.54 (m, 3H), 2.22 (dddd, J=13.08, 9.38, 7.65, 4.02 Hz, 1H), 2.12 (t, J=10.92 Hz, 1H), 1.61-1.76 (m, 1H). MS: calc'd 504 (MH.sup.+), measured 504 (MH.sup.+).

Example 7

Methyl (4R)-6-[[(8aR)-3-oxo-5,6,8,8a-tetrahydro-1H-oxazolo[3,4-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(44) ##STR00038##

(45) The title compound was prepared in analogy to Example 1 by using 2-bromo-4-fluorobenzaldehyde and (8aR)-2,3,4,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazin-6-one (for its synthesis, refer to: Tang P., et al, PCT Int. Appl., 2012019426) instead of 2-chloro-4-fluorobenzaldehyde and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 7 was obtained as a light yellow solid (19 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.26 Hz, 1H), 7.76 (d, J=3.01 Hz, 1H), 7.35-7.48 (m, 2H), 7.11 (td, J=8.41, 2.76 Hz, 1H), 6.17 (s, 1H), 4.09-4.19 (m, 1H), 4.05 (dd, J=12.80, 2.76 Hz, 1H), 3.93-4.01 (m, 1H), 3.89 (dtd, J=10.63, 7.23, 7.23, 3.76 Hz, 1H), 3.61 (s, 3H), 2.95-3.18 (m, 3H), 2.31-2.54 (m, 3H), 2.15-2.28 (m, 1H), 2.08 (t, J=10.92 Hz, 1H), 1.61-1.75 (m, 1H). MS: calc'd 548 (MH.sup.+), measured 548 (MH.sup.+).

Example 8

Ethyl (4R)-6-[[(8aR)-6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl]methyl]-4-(2-bromo-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(46) ##STR00039##

(47) The title compound was prepared in analogy to Example 1 by using 2-bromo-4-fluorobenzaldehyde, ethyl acetoacetate and (8aR)-2,3,4,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazin-6-one (for its synthesis, refer to: Tang P., et al, PCT Int. Appl., 2012019426) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 8 was obtained as a light yellow solid (8 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.01 Hz, 1H), 7.76 (d, J=3.26 Hz, 1H), 7.36-7.53 (m, 2H), 7.12 (td, J=8.34, 2.64 Hz, 1H), 6.18 (s, 1H), 4.11-4.17 (m, 1H), 4.05 (q, J=6.86 Hz, 2H), 3.97 (d, J=16.81 Hz, 1H), 3.83-3.93 (m, 1H), 2.94-3.19 (m, 3H), 2.30-2.53 (m, 3H), 2.22 (ddd, J=16.75, 12.86, 3.51 Hz, 1H), 2.07 (t, J=10.67 Hz, 1H), 1.61-1.75 (m, 1H), 1.15 (t, J=7.15 Hz, 3H). MS: calc'd 562 (MH.sup.+), measured 562 (MH.sup.+).

Example 9

Ethyl (4S)-6-[[(8aR)-6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazin-2-yl]methyl]-4-(3,4-difluorophenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(48) ##STR00040##

(49) The title compound was prepared in analogy to Example 1 by using 3,4-difluorobenzaldehyde, ethyl acetoacetate and (8aR)-2,3,4,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazin-6-one (for its synthesis, refer to: Tang P., et al, PCT Int. Appl., 2012019426) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 9 was obtained as a light yellow solid (11 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.94-8.05 (m, 1H), 7.81 (d, J=3.01 Hz, 1H), 7.14-7.33 (m, 3H), 5.74 (s, 1H), 4.07-4.23 (m, 3H), 4.03 (dd, J=13.05, 2.51 Hz, 1H), 3.83-3.94 (m, 2H), 3.09 (t, J=12.55 Hz, 1H), 2.98 (d, J=10.54 Hz, 2H), 2.28-2.52 (m, 3H), 2.20 (ddt, J=17.00, 9.29, 3.80, 3.80 Hz, 1H), 2.05 (t, J=10.92 Hz, 1H), 1.59-1.73 (m, 1H), 1.24 (t, J=7.15 Hz, 3H). MS: calc'd 502 (MH.sup.+), measured 502 (MH.sup.+).

Example 10

Ethyl (4R)-4-(2-chloro-3-fluoro-phenyl)-6-[(6-oxo-3,4,7,8,9,9a-hexahydro-1H-pyrido[1,2-a]pyrazin-2-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(50) ##STR00041##

(51) The title compound was prepared in analogy to Example 1 by using 2-chloro,3-fluorobenzaldehyde, ethyl acetoacetate and 1,2,3,4,7,8,9,9a-octahydropyrido[1,2-a]pyrazin-6-one hydrochloride salt (J & W Pharm Lab, CAS number: 151665-85-9; for its synthesis, please refer to: Ghelardini C. et al. PCT Int. Appl., 2009103176) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example was obtained as a light yellow solid (6 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.02 (d, J=3.26 Hz, 1H), 7.96 (d, J=3.26 Hz, 1H), 7.23-7.38 (m, 2H), 7.10-7.21 (m, 1H), 6.23 (s, 1H), 4.56-4.78 (m, 2H), 3.82-4.21 (m, 5H), 3.05-3.28 (m, 4H), 2.40 (m, 2H), 1.5-2.21 (m, 4H), 1.21 (m, 3H). MS: calc'd 532 (MH.sup.+), measured 532 (MH.sup.+).

Example 11

Methyl (4R)-6-[[(8aR)-3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(52) ##STR00042##

(53) The title compound was prepared in analogy to Example 1 by using (8aS)-2,5,6,7,8,8a-hexahydro-1H-imidazo[1,5-a]pyrazin-3-one (Compound H) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 11 was obtained as a light yellow solid (11 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.93-8.01 (m, 1H), 7.72-7.81 (m, 1H), 7.38-7.49 (m, 1H), 7.24 (dd, J=8.66, 2.64 Hz, 1H), 7.06 (td, J=8.47, 2.64 Hz, 1H), 6.18 (s, 1H), 4.04-4.17 (m, 1H), 3.95-4.02 (m, 1H), 3.89-3.95 (m, 1H), 3.84 (dd, J=13.43, 2.38 Hz, 1H), 3.61 (s, 3H), 3.53 (t J=8.91 Hz, 1H), 3.11-3.21 (m, 1H), 3.06 (dd, J=9.41, 4.64 Hz, 1H), 2.92 (dd, J=9.79, 1.51 Hz, 1H), 2.84 (dd, J=11.17, 2.64 Hz, 1H), 2.38 (td, J=11.67, 3.51 Hz, 1H), 2.19-2.29 (m, 1H). MS: calc'd 505 (MH.sup.+), measured 505 (MH.sup.+).

(54) ##STR00043##

Preparation of (8aS)-2,5,6,7,8,8a-hexahydro-1H-imidazo[1,5-a]pyrazin-3-one (Compound H)

(55) ##STR00044##
Step 1:

(56) To a stirred solution of Na.sub.2CO.sub.3 (118 g, 1.11 mol) in water (600 mL) was added (2S)-piperazine-2-carboxylic acid dihydrochloride salts (30 g, 0.15 mol) at 25 C., followed by di-tert-butyl dicarbonate (112 g, 0.56 mol) and tetrahydrofuran (300 mL). Then the mixture was stirred for additional 20 hours at room temperature. The reaction mixture was concentrated in vacuo and the resulting solution was extracted with methyl tert-butyl ether (200 mL) three times to remove nonpolar species. The aqueous layer was cooled to below 0 C. and then treated with 3.0 M HCl until to pH 2 to 3. Then it was extracted with ethyl acetate (500 mL) three times. The combined organic layer was dried over Na.sub.2SO.sub.4, and then filtered and concentrated under vacuum to give Compound I (39 g).

(57) Step 2:

(58) To a solution of Compound I (39 g, 0.12 mol) in tetrahydrofuran (200 mL) was added dropwise BH.sub.3.THF complex (240 mL, 0.24 mol) at 0 C. under N.sub.2 atmosphere. Then the mixture was warmed to room temperature and heated to reflux for 2-3 hours. The mixture was quenched with MeOH (100 mL) at 0 C. and the obtained solution was concentrated in vacuo. The residue was re-dissolved into MeOH (200 mL) and the solution was refluxed for 3 to 4 hours. Then the solvent was removed to give the crude product (45 g). The crude product was purified by column chromatography on silica (petroleum ether/EtOAc=10:1 to 1:1) to give Compound J (31 g).

(59) Step 3:

(60) To a solution of Compound J (15 g, 47.41 mmol), Ph.sub.3P (16.17 g, 61.63 mmol) and phthalimide (9.07 g, 61.63 mmol) in anhydrous tetrahydrofuran (230 mL) was added a solution of diisopropyl azodicarboxylate (12.46 g, 61.63 mmol) in tetrahydrofuran (20 mL) at 10 C. under N.sub.2 atmosphere. Then the mixture was stirred for additional 2 hours at 10 C. The mixture was quenched with 1N HCl (100 mL) and then extracted with EtOAc (300 mL). The organic layer was dried over Na.sub.2SO.sub.4, filtered, and then concentrated in vacuo to give the crude product (50 g). The crude product was triturated with petroleum ether/EtOAc (200 mL, V/V=5:1) to get crude product (25 g) with removal of triphenylphosphine oxide and other impurities. The crude product was purified by column chromatography on silica (petroleum ether/EtOAc=20:1) to get the pure product K (12 g) as a white solid and some impure compound K (4 g).

(61) Step 4:

(62) To a stirred solution of Compound K (12 g, 26.9 mmol) in ethanol (85 mL) was added methylamine alcohol solution (85 mL) at room temperature. Then the mixture was heated to reflux for additional 3 hours. The mixture was cooled to room temperature and then concentrated. The resulting residue was dissolved in water (50 mL), then acidified to pH 3 with 1N HCl solution, and then extracted with methyl-tert-butyl ether (50 mL) three times. The pH was adjusted to 10 with solid NaOH and the mixture was extracted with ethyl acetate (150 mL) three times. The organic layer was dried and filtered and the solvent was removed under reduced pressure to give Compound L (6 g crude).

(63) Step 5:

(64) To a stirred suspension of sodium hydride (1.0 g, 25 mmol) in tetrahydrofuran (50 mL) was added Compound L (6 g, 19 mmol, crude) at 0 C. Then the mixture was heated to reflux for 2 hours. And then additional NaH (1.0 g, 25 mmol) was added into the mixture at room temperature. After the reaction mixture was refluxed for 1 hour, the reaction was quenched with ice-water (100 mL) and then extracted with EtOAc (300 mL). The organic layer was evaporated in vacuo to give the crude product (5 g). The crude product was purified by column chromatography on silica gel (EtOAc/MeOH=200:1) to give Compound M (2.2 g, 40%).

(65) Step 6:

(66) To a stirred solution of Compound M (2.2 g, 9.1 mmol) in 30 mL of dichloromethane was added dropwise 10 mL of TFA. After the reaction mixture was stirred for 1 hour, the solvent was removed in vacuo to give (8aS)-2,5,6,7,8,8a-hexahydro-1H-imidazo[1,5-a]pyrazin-3-one trifluoro acetitic acid salt (Compound H) as a crude product which was used directly without further purification.

Example 12

Methyl (4R)-6-[[(8aS)-3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(67) ##STR00045##

(68) The title compound was prepared in analogy to Example 11 by using (2R)-piperazine-2-carboxylic acid instead of (2S)-piperazine-2-carboxylic acid. Example 12 was obtained as a light yellow solid (13 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.91-8.01 (m, 1H), 7.71-7.81 (m, 1H), 7.42 (dd, J=8.66, 6.15 Hz, 1H), 7.24 (dd, J=8.66, 2.64 Hz, 1H), 7.05 (td, J=8.41, 2.51 Hz, 1H), 6.18 (s, 1H), 4.12 (d, J=17.32 Hz, 1H), 3.95-4.05 (m, 1H), 3.91 (d, J=17.32 Hz, 1H), 3.77 (dd, J=13.18, 1.88 Hz, 1H), 3.61 (s, 3H), 3.56-3.60 (m, 1H), 3.08-3.21 (m, 2H), 3.01 (dd, J=10.79, 2.76 Hz, 1H), 2.74 (d, J=11.29 Hz, 1H), 2.39 (t, J=10.92 Hz, 1H), 2.24 (td, J=11.67, 3.26 Hz, 1H). MS: calc'd 505 (MH.sup.+), measured 505 (MH.sup.+).

Example 13

Ethyl (4R)-6-[[(8aS)-3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-bromo-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(69) ##STR00046##

(70) The title compound was prepared in analogy to Example 11 by using 2-bromo-4-fluorobenzaldehyde and ethyl acetoacetate instead of 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate. Example 13 was obtained as a light yellow solid (34 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.91-8.02 (m, 1H), 7.78 (d, J=3.01 Hz, 1H), 7.34-7.53 (m, 2H), 7.12 (td, J=8.41, 2.76 Hz, 1H), 6.18 (s, 1H), 4.15-4.31 (m, 1H), 3.93-4.13 (m, 4H), 3.76-3.89 (m, 1H), 3.61 (t, J=8.91 Hz, 1H), 3.07-3.25 (m, 3H), 2.83-3.03 (m, 1H), 2.55 (br. s., 1H), 2.40 (br. s., 1H), 1.14 (t, J=7.15 Hz, 3H). MS: calc'd 563 (MH.sup.+), measured 563 (MH.sup.+).

Example 14

Ethyl (4R)-6-[[(8aR)-3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-bromo-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(71) ##STR00047##

(72) The title compound was prepared in analogy to Example 12 by using 2-bromo-4-fluorobenzaldehyde and ethyl acetoacetate instead of 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate. Example 14 was obtained as a light yellow solid (34 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.92-8.01 (m, 1H), 7.78 (d, J=3.26 Hz, 1H), 7.35-7.56 (m, 2H), 7.12 (td, J=8.41, 2.76 Hz, 1H), 6.18 (s, 1H), 4.17-4.32 (m, 1H), 3.96-4.16 (m, 4H), 3.89 (dd, J=13.55, 2.51 Hz, 1H), 3.49-3.64 (m, 1H), 3.17-3.28 (m, 1H), 2.97-3.17 (m, 3H), 2.31-2.68 (m, 2H), 1.14 (t, J=7.15 Hz, 3H). MS: calc'd 563 (MH.sup.+), measured 563 (MH.sup.+).

Example 15

Methyl (4R)-6-[[(8aR)-1,3-dioxo-5,6,8,8a-tetrahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(73) ##STR00048##

(74) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,3-dioxo-5,6,8,8a-tetrahydroimidazo[1,5-a]pyrazin-7-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound N). Example 15 was obtained as a yellow solid (11 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.89-7.97 (m, 1H), 7.71-7.81 (m, 1H), 7.38-7.53 (m, 1H), 7.24 (dd, J=8.78, 2.76 Hz, 1H), 7.07 (td, J=8.41, 2.76 Hz, 1H), 6.19 (s, 1H), 4.28-4.40 (m, 1H), 4.15-4.26 (m, 1H), 3.92-4.07 (m, 2H), 3.61 (s, 3H), 3.36 (d, J=4.77 Hz, 1H), 3.20-3.29 (m, 1H), 2.78-2.86 (m, 1H), 2.42 (t, J=11.17 Hz, 1H), 2.29 (td, J=11.86, 3.64 Hz, 1H). MS: calc'd 519 (MH.sup.+), measured 519 (MH.sup.+).

(75) ##STR00049##

Preparation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,3-dioxo-5,6,8,8a-tetrahydroimidazo[1,5-a]pyrazin-7-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound N)

(76) The title compound was prepared in analogy to Example 1 by using 6,7,8,8a-tetrahydro-5H-imidazo[1,5-a]pyrazine-1,3-dione (For its synthesis, please refer to: WO2010/23480) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D).

Example 16

Methyl (4R)-6-[[(8aS)-1,3-dioxo-5,6,8,8a-tetrahydroimidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(77) ##STR00050##

(78) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,3-dioxo-5,6,8,8a-tetrahydroimidazo[1,5-a]pyrazin-7-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound N) in Example 15. Example 16 was obtained as a yellow solid (10 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.90-7.97 (m, 1H), 7.76 (d, J=3.26 Hz, 1H), 7.37-7.49 (m, 1H), 7.24 (dd, J=8.78, 2.76 Hz, 1H), 7.06 (td, J=8.41, 2.76 Hz, 1H), 6.19 (s, 1H), 4.24-4.36 (m, 1H), 4.13-4.22 (m, 1H), 4.09 (dd, J=13.43, 2.89 Hz, 1H), 3.98-4.06 (m, 1H), 3.61 (s, 3H), 3.27 (td, J=12.67, 3.76 Hz, 1H), 3.18 (dd, J=11.04, 4.27 Hz, 1H), 2.97-3.04 (m, 1H), 2.44 (td, J=11.80, 3.76 Hz, 1H), 2.29 (t, J=11.17 Hz, 1H). MS: calc'd 519 (MH.sup.+), measured 519 (MH.sup.+).

Example 17

Methyl (4R)-6-[[(3aS)-1,1-dioxo-2,3,3a,4,6,7-hexahydro-[1,2,5]thiadiazolo[2,3-a]pyrazin-5-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(79) ##STR00051##

(80) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,1-dioxo-2,3,3a,4,6,7-hexahydro-[1,2,5]thiadiazolo[2,3-a]pyrazin-5-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound O). Example 17 was obtained as a yellow solid (14 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.93-8.00 (m, 1H), 7.77 (d, J=3.01 Hz, 1H), 7.44 (dd, J=8.78, 6.02 Hz, 1H), 7.25 (dd, J=8.66, 2.64 Hz, 1H), 7.06 (td, J=8.41, 2.76 Hz, 1H), 6.18 (s, 1H), 4.12-4.26 (m, 1H), 3.97-4.10 (m, 1H), 3.58-3.70 (m, 4H), 3.42-3.55 (m, 2H), 3.03-3.20 (m, 4H), 2.63-2.76 (m, 1H), 2.42 (t, J=10.54 Hz, 1H). MS: calc'd 541 (MH.sup.+), measured 541 (MH.sup.+).

(81) ##STR00052##

Preparation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,1-dioxo-2,3,3a,4,6,7-hexahydro-[1,2,5]thiadiazolo[2,3-a]pyrazin-5-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound O)

(82) The title compound was prepared in analogy to Example 1 by using hexahydro-1-thia-2,5,7a-triaza-indene 1,1-dioxide (Compound P) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D).

Preparation of hexahydro-1-thia-2,5,7a-triaza-indene 1,1-dioxide (Compound P)

(83) ##STR00053##

(84) To a stirred solution of 3-aminomethyl-piperazine-1-carboxylic acid tert-butyl ester (CAS number: 1376099-80-7, Bepharm) (215 mg, 1.0 mmol) in 2 mL of pyridine was added sulfamide (1.5 mmol). The reaction mixture was heated to 150 C. for 6 hours under microwave. The solvent was removed in vacuo, and the residue was dissolved in 3 mL of dichloromethane and 1 mL of TFA. The reaction mixture was stirred for 1 hour, and the solvent was removed in vacuo to give the crude Compound P which was used directly without further purification. LC/MS: calc'd 178 (MH.sup.+), measured 178 (MH.sup.+).

Example 18

Methyl (4R)-6-[[(3aR)-1,1-dioxo-2,3,3a,4,6,7-hexahydro-[1,2,5]thiadiazolo[2,3-a]pyrazin-5-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(85) ##STR00054##

(86) The title compound was prepared by SFC chiral separation of methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[(1,1-dioxo-2,3,3a,4,6,7-hexahydro-[1,2,5]thiadiazolo[2,3-a]pyrazin-5-yl)methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound O) in Example 17. Example 18 was obtained as a yellow solid (28 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.26 Hz, 1H), 7.79 (d, J=3.26 Hz, 1H), 7.45 (dd, J=8.78, 6.02 Hz, 1H), 7.25 (dd, J=8.78, 2.51 Hz, 1H), 7.07 (td, J=8.41, 2.76 Hz, 1H), 6.19 (s, 1H), 4.32 (d, J=16.81 Hz, 1H), 4.09 (d, J=16.81 Hz, 1H), 3.67-3.81 (m, 1H), 3.58-3.66 (m, 3H), 3.46-3.58 (m, 2H), 3.29-3.31 (m, 1H), 3.23 (dd, J=9.79, 8.78 Hz, 1H), 3.02-3.19 (m, 2H), 2.69 (q, J=10.79 Hz, 2H). MS: calc'd 541 (MH.sup.+), measured 541 (MH.sup.+).

Example 19

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(87) ##STR00055##

(88) The title compound was prepared in analogy to Example 1 by using 2-chloro,3-fluorobenzaldehyde, ethyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 19 was obtained as a light yellow solid (12 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.92-8.02 (m, 1H), 7.70-7.80 (m, 1H), 7.21-7.36 (m, 2H), 7.10-7.21 (m, 1H), 6.19-6.28 (m, 1H), 3.99-4.14 (m, 3H), 3.81-3.96 (m, 3H), 3.47-3.56 (m, 1H), 3.38-3.44 (m, 1H), 3.27-3.32 (m, 1H), 3.15-3.25 (m, 1H), 3.07-3.14 (m, 1H), 2.79-2.96 (m, 2H), 2.30-2.41 (m, 1H), 2.13-2.23 (m, 1H), 1.20 (d, J=2.76 Hz, 6H), 1.13 (m, 3H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Preparation of 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid (Compound Q)

(89) ##STR00056##
Step 1:

(90) To a stirred solution of (R)-4-N-boc-2-hydroxymethyl-piperazine (CAS number: 314741-40-7, Bepharm; for its synthesis, please refer to: Gao H., Renslo A. R. J. Org. Chem. 2007, 72, 8591-8592) (6 g, 27.8 mmol) in saturated NaHCO.sub.3 (45 mL) and EtOAc (45 mL) was added benzyl chloroformate (7.13 g, 41.7 mmol) dropwise at 0 C. Then the reaction mixture was stirred at room temperature for 15 hours. The reaction was diluted with EtOAc (60 mL), and the organic layer was separated and aqueous layer was extracted with EtOAc (35 mL). The combined organic layer was dried over Na.sub.2SO.sub.4 and the solution was concentrated to give a crude product. The crude product was purified by column chromatography on silica (Petroleum ether:EtOAc=10:1 to 1:1) to give Compound R (6.7 g).

(91) Step 2:

(92) To a stirred solution of oxalyl chloride (3.64 g, 28.65 mmol) in anhydrous dichloromethane (80 mL) at 78 C. was added dimethyl sulfoxide (4.47 g, 57.3 mmol) dropwise. After 10 minutes, a solution of Compound R (6.7 g, 19.1 mmol) in dichloromethane (20 mL) was added dropwise. After the mixture was stirred for 30 minutes at 78 C., N,N-diisopropylethylamine (14.78 g, 114.6 mmol) was added and the reaction mixture was stirred for 30 minutes. After the reaction mixture was slowly warmed to 0 C. over 30 minutes, it was diluted with dichloromethane (80 mL), washed with 5% aqueous citric acid (10 mL), brine and then dried over Na.sub.2SO.sub.4. After filtration, the mixture was concentrated in vacuo to get the crude product S (7 g).

(93) Step 3:

(94) To a stirred solution of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt (3.4 g, 18.6 mmol) in anhydrous DCM (100 mL) was added DIPEA (2.6 g, 27.3 mmol) at room temperature. Then Compound S (7 g, 20 mmol) was added, followed by NaBH(OAc).sub.3 (6.3 g, 29.8 mmol) and AcOH (1.5 mL). The reaction mixture was stirred for 16 hours at room temperature. Water (100 mL) was added and the mixture was extracted with DCM (100 mL). The organic layer was dried and concentrated in vacuo to dryness to give crude Compound T (7.3 g).

(95) Step 4:

(96) To the solution of Compound T (3.3 g, 6.9 mmol) in EtOH (100 mL) was added Pd/C (1 g). Then the mixture was stirred at 50 C. for 3 hours under hydrogen atmosphere (50 Psi). The reaction mixture was filtered and the filtrate was concentrated in vacuo to get the Compound U (1.8 g).

(97) Step 5:

(98) To the solution of Compound U (1.8 g, 5.24 mmol) in anhydrous dichloromethane (60 mL) was added N,N-diisopropylethylamine (3.4 g, 26.2 mmol) at 0 C. Then triphosgene (783 mg, 2.62 mmol) was added at 0 C. and the mixture was stirred at 10-15 C. for 16 hours. Water (50 mL) was added and the mixture was extracted with dichloromethane (60 mL), and then dried over Na.sub.2SO.sub.4. The solvent was removed in vacuo to give the crude product. The crude product was purified by column chromatography on silica (petroleum ether:EtOAc=5:1 to 1:1) to obtain Compound V (1.3 g).

(99) Step 6:

(100) To a stirred solution of Compound V (240 mg, 0.64 mmol) in THF (3 ml) was added a solution of LiOH.H.sub.2O (215 mg, 5.10 mmol) in H.sub.2O (1 mL) at room temperature. After the reaction mixture was stirred at room temperature overnight, it was acidified to PH 3 to 4 with 1 N HCl at 0 C. The mixture was then concentrated and azeotropically dried with toluene to give the crude product which was dissolved in dichloromethane (2 mL) and treated with trifluoroacetic acid (2 mL) at room temperature. After the reaction mixture was stirred at room temperature for 1 hour, the solvent was removed in vacuo to give 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) which was used directly.

Example 20

3-[(8aS)-7-[[(4R)-4-(2-chlorophenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2-methyl-propanoic Acid

(101) ##STR00057##

(102) The title compound was prepared in analogy to Example 19 by using 2-chlorobenzaldehyde and methyl 3-amino-2-methyl-propanoate hydrochloride salt (Compound W) instead of 2-chloro-4-fluorobenzaldehyde and ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 20 was obtained as a light yellow solid (20 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.01 Hz, 1H), 7.75 (d, J=3.01 Hz, 1H), 7.42 (d, J=7.78 Hz, 2H), 7.14-7.36 (m, 2H), 6.22 (s, 1H), 3.97-4.16 (m, 3H), 3.79-3.97 (m, 3H), 3.43-3.59 (m, 2H), 3.26-3.43 (m, 2H), 3.05-3.26 (m, 2H), 2.80-2.99 (m, 2H), 2.65-2.80 (m, 1H), 2.35 (t J=10.79 Hz, 1H), 2.19 (q, J=10.46 Hz, 1H), 1.06-1.24 (m, 5H). MS: calc'd 587 (MH.sup.+), measured 587 (MH.sup.+).

Preparation of Methyl 3-amino-2-methyl-propanoate Hydrochloride Salt (Compound W)

(103) ##STR00058##

(104) To the solution of DL-3-aminoisobutyric acid (CAS number: 144-90-1, Aldrich) (2.7 g, 26.4 mmol) in MeOH (50 mL) was added SOCl.sub.2 (6.17 g, 52.8 mmol) dropwise at 0 C. under N.sub.2 atmosphere. The reaction was refluxed for 18 hours. The solvent was removed in vacuo to give crude Compound W which was used directly in the next step.

Example 21

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2-methyl-propanoic Acid

(105) ##STR00059##

(106) The title compound was prepared in analogy to Example 19 by using methyl 3-amino-2-methyl-propanoate hydrochloride salt (Compound W) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 21 was obtained as a light yellow solid (10 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.26 Hz, 1H), 7.76 (d, J=3.26 Hz, 1H), 7.21-7.36 (m, 2H), 7.10-7.21 (m, 1H), 6.24 (s, 1H), 3.98-4.17 (m, 3H), 3.79-3.98 (m, 3H), 3.42-3.58 (m, 2H), 3.24-3.42 (m, 2H), 3.08-3.24 (m, 2H), 2.79-2.96 (m, 2H), 2.73 (ddt, J=10.63, 7.00, 3.73, 3.73 Hz, 1H), 2.30-2.43 (m, 1H), 2.13-2.27 (m, 1H), 1.08-1.24 (m, 5H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Example 22

3-[(8aS)-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(107) ##STR00060##

(108) The title compound was prepared in analogy to Example 19 by using 2-methyl-3,4-difluorobenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde. Example 22 was obtained as a light yellow solid (2 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.88-8.00 (m, 1H), 7.69-7.80 (m, 1H), 6.95-7.13 (m, 2H), 5.88-5.99 (m, 1H), 4.03-4.16 (m, 2H), 3.79-3.97 (m, 3H), 3.47-3.54 (m, 1H), 3.36-3.44 (m, 2H), 3.28-3.31 (m, 1H), 3.07-3.24 (m, 2H), 2.76-2.94 (m, 2H), 2.57 (d, J=2.26 Hz, 3H), 2.31-2.42 (m, 1H), 2.12-2.22 (m, 1H), 1.10-1.24 (m, 9H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Example 23

Ethyl (4R)-4-(2-chloro-3-fluoro-phenyl)-6-[[2-(2-methoxy-1,1-dimethyl-2-oxo-ethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(109) ##STR00061##

(110) The title compound was prepared in analogy to Example 19 by using methyl 3-amino-2-methyl-propanoate hydrochloride salt instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 23 was obtained as a light yellow solid (4 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.94-8.03 (m, 1H), 7.72-7.82 (m, 1H), 7.22-7.38 (m, 2H), 7.11-7.20 (m, 1H), 6.25 (s, 1H), 4.10-4.22 (m, 1H), 3.85-4.02 (m, 2H), 3.76-3.84 (m, 1H), 3.74 (d, J=1.00 Hz, 2H), 3.59-3.71 (m, 1H), 3.12-3.30 (m, 2H), 2.76-3.03 (m, 2H), 2.20-2.52 (m, 2H), 1.50 (t, J=7.78 Hz, 6H), 1.14 (t, J=7.03 Hz, 3H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Example 24

Methyl 7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-2,5,6,8-tetrahydro-1H-imidazo[1,5-a]pyrazine-8a-carboxylate

(111) ##STR00062##

(112) The title compound was prepared in analogy to Example 1 by using 2-chloro,3-fluorobenzaldehyde, ethyl acetoacetate and methyl 3-oxo-1,2,5,6,7,8-hexahydroimidazo[1,5-a]pyrazine-8a-carboxylate (Compound X) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 24 was obtained as a light yellow solid (5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.26 Hz, 1H), 7.76 (d, J=3.26 Hz, 1H), 7.27-7.35 (m, 1H), 7.26 (s, 1H), 7.16 (s, 1H), 6.22 (s, 1H), 3.97-4.14 (m, 3H), 3.87-3.95 (m, 1H), 3.78-3.85 (m, 1H), 3.67 (s, 3H), 3.46 (d, J=9.79 Hz, 2H), 3.35-3.42 (m, 2H), 2.87-2.99 (m, 1H), 2.45-2.55 (m, 1H), 2.29-2.42 (m, 1H), 1.12 (t, J=7.15 Hz, 3H). MS: calc'd 577 (MH.sup.+), measured 577 (MH.sup.+).

Preparation of Methyl 3-oxo-1,2,5,6,7,8-hexahydroimidazo[1,5-a]pyrazine-8a-carboxylate (Compound X)

(113) ##STR00063##
Step 1:

(114) Piperazine-1,3-dicarboxylic acid 1-tert-butyl ester 3-methyl ester (244 mg, 1.0 mmol) was dissolved in 5 mL of dichloromethane followed by sequential addition of TEA (202 mg, 2.0 mmol) and benzyl chloroformate (170 mg, 1.0 mmol) at room temperature. After the reaction was stirred for 1 hour, it was diluted with dichloromethane, and then washed with water and brine. The solvent was concentrated to give crude Compound Y, which was used directly without further purification.

(115) Step 2:

(116) Piperazine-1,2,4-tricarboxylic acid 1-benzyl ester 4-tert-butyl ester 2-methyl ester (Compound Y) from previous step was dissolved in 5 mL of THF at 78 C., then LDA (2 M, 1 mL, 2 mmol) was added dropwise. After the reaction mixture was stirred for 2 hours at 78 C., 2-bromomethyl-isoindole-1,3-dione (239 mg, 1 mmol) in 2 mL of THF was added. The reaction mixture was stirred overnight. The solvent was removed in vacuo and the residue was triturate with petroleum ether. The solid was collected by filtration, washed with water and then dried in vacuo to give Compound Z.

(117) Step 3:

(118) 2-(1,3-Dioxo-1,3-dihydro-isoindol-2-ylmethyl)-piperazine-1,2,4-tricarboxylic acid 1-benzyl ester 4-tert-butyl ester 2-methyl ester (Compound Z) from Step 2 was dissolved in 3 mL of EtOH and 1 mL of N.sub.2H.sub.4 solution. The reaction mixture was stirred at room temperature overnight. The solid was filtered off and the filtrate was concentrated in vacuo. The crude oil was dissolved in 10 mL of MeOH and stirred with 50 mg of Pd/C under H.sub.2 atmosphere overnight. After filtration, the solvent was concentrated in vacuo to give 100 mg of crude Compound AA as oil, which was used directly without further purification in the next step.

(119) Step 4:

(120) 3-Aminomethyl-piperazine-1,3-dicarboxylic acid 1-tert-butyl ester 3-methyl ester (Compound AA) from Step 3 was dissolved in 4 mL of dichloromethane and 1 mL of DIPEA, then triphosgene (108 mg, 0.36 mmol) was added at room temperature. After the reaction mixture was stirred for 30 minutes, the solvent was removed in vacuo. The residue was dissolved in 3 mL of dichloromethane and 1 mL of TFA. The reaction mixture was stirred for 1 hour at room temperature, and the solvent was removed in vacuo to give the crude Compound X which was used directly without further purification.

Example 25

(R)-6-[(S)-2-(4-Carboxy-phenyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester

(121) ##STR00064##

(122) The title compound was prepared in analogy to Example 1 by using 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AB) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 25 was obtained as a light yellow solid (45 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.93-8.06 (m, 3H), 7.79 (d, 1H), 7.64-7.75 (m, 2H), 7.45 (m, 1H), 7.25 (m, 1H), 7.08 (m, 1H), 6.19 (s, 1H), 4.22 (d, 1H), 3.95-4.15 (m, 4H), 3.53-3.66 (m, 4H), 3.29 (d, 1H), 3.09 (d, 2H), 3.03-3.17 (m, 2H), 2.50-2.63 (m, 1H), 2.35 (br. s., 1H). MS: calc'd 625 (MH.sup.+), measured 625 (MH.sup.+).

Preparation of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic Acid (Compound AB)

(123) ##STR00065## ##STR00066##
Step 1:

(124) A mixture of tert-butyl (8aR)-3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazine-7-carboxylate (Compound M, 200 mg, 0.83 mmol), xantphos (46 mg, 0.08 mmol), Cs.sub.2CO.sub.3 (378 mg, 1.16 mmol), Pd(OAc).sub.2 (18 mg, 0.08 mmol) and 4-iodo-benzoic acid methyl ester (282 mg, 1.08 mmol) in dioxane (6 mL) was degassed with argon atmosphere three times. The mixture was stirred at room temperature for 5 minutes under argon atmosphere, and then heated at 90 C. overnight. The mixture was diluted with EtOAc (20 mL), and filtered through celite. The filtrate was concentrated and purified on silica gel to give Compound AC (240 mg).

(125) Step 2:

(126) To a solution of Compound AC (240 mg, 0.64 mmol) in THF (3 mL) was added a solution of LiOH.H.sub.2O (215 mg, 5.10 mmol) in H.sub.2O (1 mL) at room temperature. After the reaction mixture was stirred at room temperature overnight, it was acidified to PH 3 to 4 with 1 N HCl at 0 C. The mixture was then concentrated and azeotropically dried with toluene to give Compound AD (231 mg) which was used directly without further purification in the next step.

(127) Step 3:

(128) To a solution of Compound AD (231 mg, 0.64 mmol) in dichloromethane (2 mL) was added TFA (2 mL) at room temperature. The mixture was stirred at room temperature for 1 hour, then concentrated to give methyl 4-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)benzoic acid (Compound AB) (240 mg).

Example 26

(R)-6-[(S)-2-(4-Carboxy-phenyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(129) ##STR00067##

(130) The title compound was prepared in analogy to Example 25 by using 2-chloro,3-fluorobenzaldehyde and ethyl acetoacetate instead of 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate. Example 26 was obtained as a light yellow solid (76 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.85-7.96 (m, 3H), 7.79 (d, 1H), 7.61 (d, 2H), 7.23-7.30 (m, 2H), 7.08-7.19 (m, 1H), 6.16 (s, 1H), 4.62 (d, 1H), 4.43 (d, 1H), 4.22 (m, 1H), 4.02-4.16 (m, 2H), 3.97 (m, 2H), 3.57-3.73 (m, 3H), 3.36-3.49 (m, 1H), 3.02-3.16 (m, 2H), 0.95-1.08 (m, 3H). MS: calc'd 639 (MH.sup.+), measured 639 (MH.sup.+).

Example 27

(R)-6-[(S)-2-(3-Carboxy-phenyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(131) ##STR00068##

(132) The title compound was prepared in analogy to Example 25 by using 2-chloro,3-fluorobenzaldehyde, ethyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AE) instead of 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AB). Example 27 was obtained as a light yellow solid (20 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.11 (s, 1H), 7.90 (d, 1H), 7.75-7.82 (m, 2H), 7.66 (d, 1H), 7.37 (m, 1H), 7.23-7.29 (m, 2H), 7.07-7.17 (m, 1H), 6.16 (s, 1H), 4.59 (d, 1H), 4.34-4.45 (m, 1H), 4.15-4.25 (m, 1H), 4.01-4.13 (m, 2H), 3.97 (m, 2H), 3.52-3.67 (m, 3H), 3.36-3.46 (m, 1H), 3.02-3.16 (m, 2H), 1.01 (m, 3H). MS: calc'd 639 (MH.sup.+), measured 639 (MH.sup.+).

Preparation of 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic Acid (Compound AE)

(133) Compound AE was prepared in analogy to Compound AB in Example 25 by using 3-iodo-benzoic acid methyl ester instead of 4-iodo-benzoic acid methyl ester.

Example 28

(R)-6-[(S)-2-(2-Carboxy-phenyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid ethyl Ester

(134) ##STR00069##

(135) The title compound was prepared in analogy to Example 25 by using 2-chloro,3-fluorobenzaldehyde and ethyl acetoacetate and 2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AF) instead of 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate and 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AB). Example 28 was obtained as a light yellow solid (95 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.83-7.93 (m, 2H), 7.80 (d, 1H), 7.53 (m, 1H), 7.24-7.35 (m, 4H), 7.08-7.19 (m, 1H), 6.16 (s, 1H), 4.66 (d, 1H), 4.48 (d, 1H), 4.20-4.29 (m, 1H), 4.06-4.11 (m, 1H), 3.92-4.04 (m, 3H), 3.65 (d, 2H), 3.29-3.55 (m, 3H), 3.13 (m, 1H), 1.02 (m, 3H). MS: calc'd 639 (MH.sup.+), measured 639 (MH.sup.+).

Preparation of 2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic Acid (Compound AF)

(136) The title compound was prepared in analogy to Compound AB in Example 25 by using 2-iodo-benzoic acid methyl ester instead of 4-iodo-benzoic acid methyl ester.

Example 29

(R)-6-[(S)-2-(3-Carboxy-phenyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester

(137) ##STR00070##

(138) The title compound was prepared in analogy to Example 25 by using 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AE) instead of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AB). Example 29 was obtained as a light yellow solid (220 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.20-8.26 (m, 1H), 8.03 (d, 1H), 7.85-7.96 (m, 2H), 7.78 (m, 1H), 7.56 (m, 1H), 7.49 (m, 1H), 7.30 (m, 1H), 7.13 (m, 1H), 6.21 (s, 1H), 4.78 (d, 1H), 4.55-4.63 (m, 1H), 4.36 (dm, 1H), 4.13-4.29 (m, 2H), 3.72-3.90 (m, 3H), 3.65 (s, 3H), 3.51-3.61 (m, 1H), 3.35-3.40 (m, 1H), 3.26-3.31 (m, 1H). MS: calc'd 625 (MH.sup.+), measured 625 (MH.sup.+).

Example 30

2-[7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-1,5,6,8-tetrahydroimidazo[1,5-a]pyrazin-8a-yl]acetic Acid

(139) ##STR00071##

(140) The title compound was prepared in analogy to Example 1 by using 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AG) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 30 was obtained as a light yellow solid (48 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.93 (d, J=3.0 Hz, 1H), 7.73 (d, J=3.0 Hz, 1H), 7.43 (dd, J=6.1, 8.7 Hz, 1H), 7.24 (dd, J=2.5, 8.8 Hz, 1H), 7.06 (d, J=2.8 Hz, 1H), 6.16 (s, 1H), 4.14 (d, J=16.6 Hz, 1H), 3.85-3.71 (m, 2H), 3.60 (s, 3H), 3.46 (d, J=9.8 Hz, 1H), 3.30-3.15 (m, 3H), 3.06 (d, J=11.3 Hz, 1H), 2.95-2.82 (m, 2H), 2.45 (d, J=5.0 Hz, 1H), 2.39-2.29 (m, 1H), 2.22 (d, J=11.5 Hz, 1H), 0.83-0.53 (m, 4H). MS: calc'd 604 (MH.sup.+), measured 604 (MH.sup.+).

Preparation of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic Acid (Compound AG)

(141) ##STR00072##
Step 1:

(142) To a solution of piperazine-1,3-dicarboxylic acid 1-tert-butyl ester 3-methyl ester (10.6 g, 43 mmol) in 250 mL of dichloromethane was added (Boc).sub.2O (19 g, 86 mmol) at 0 C. The reaction mixture stirred for 4 hours at room temperature, and then quenched with water and then extracted with dichloromethane. After the organic layer was dried over anhydrous Na.sub.2SO.sub.4, the filtrate was concentrated. The residue was purified by silica gel column chromatography to give Compound AH (yield 13.6 g, 92%).

(143) Step 2:

(144) To a solution of Compound AH (13.4 g, 38.9 mmol) in 200 mL of anhydrous THF was added freshly prepared LDA (2M in THF, 38 mL) at 78 C. The reaction mixture was stirred for 30 minutes at 78 C. and then warmed to room temperature for 30 minutes. The mixture was cooled down to 78 C. and a solution of allyl bromide (6.7 mL, 77.9 mmol) in 10 mL of THF was added. After the mixture was warmed to room temperature and stirred overnight, the solvent was removed under reduced pressure. The residue was purified by silica gel column chromatography to afford Compound AI (yield 13.6 g, 91%).

(145) Step 3:

(146) To a solution of Compound AI (1.0 g, 2.6 mmol) in 20 mL of anhydrous THF was added LAH (2M in THF, 1.3 mL) at 78 C. The reaction mixture was stirred for 30 minutes at 78 C. and then warmed to room temperature and stirred overnight. Anhydrous Na.sub.2SO.sub.4 was added. After 1 hour, the filtrate was concentrated. The residue was purified by silica gel column chromatography to afford Compound AJ (yield 0.7 g, 75%).

(147) Step 4:

(148) To a solution of Compound AJ (3 g, 8.4 mmol) in 50 mL of anhydrous dichloromethane was added Dess-Martin periodinane (6 g, 14.1 mmol) at 0 C. The reaction mixture was stirred for 2 hours at room temperature, and then quenched with water. The filtrate was extracted with dichloromethane, and then dried over anhydrous Na.sub.2SO.sub.4, and the filtrate was concentrated to give Compound AK without further purification.

(149) Step 5:

(150) To a stirred solution of Compound AK (1.5 g, 4.2 mmol) in dichloromethane (15 mL) was added successively acetic acid and cyclopropylamine (0.29 mL, 4.2 mmol). The reaction mixture was stirred for 2 hours at room temperature, then sodium triacetoxyborohydride (520 mg, 8.4 mmol) was added and the reaction mixture was stirred for 3 hours at room temperature. The reaction mixture was quenched with saturated NH.sub.4Cl. Then water (20 mL) was added followed by dichloromethane (40 mL). The organic layer was separated and then washed with brine, and then dried over Na.sub.2SO.sub.4. The filtrate was concentrated to give Compound AL without further purification.

(151) Step 6:

(152) To a solution of Compound AL (1.5 g, 3.8 mmol) in 20 mL of anhydrous THF was added NaH (0.26 g, 9.4 mmol) at 0 C. The reaction mixture was stirred over night at 85 C. Then the reaction was cooled to room temperature, quenched with water and extracted with dichloromethane. The organic layer was dried over anhydrous Na.sub.2SO.sub.4 and then concentrated in vacuo. The residue was purified by silica gel column chromatography to give Compound AM (yield 1.0 g, 82%).

(153) Step 7:

(154) To a solution of Compound AM (700 mg, 2.2 mmol) in H.sub.2O (1 mL) and CH.sub.3CN (6 mL) under nitrogen atmosphere was added RuCl.sub.3.H.sub.2O (18 mg, 0.086 mmol), followed by NaIO.sub.4 (2.7 g, 12.6 mmol). The reaction was stirred at room temperature overnight. The mixture was diluted with water and ethyl ether, filtered through a celite pad, and the pad was washed with ethyl ether. The aqueous layer was extracted with dichloromethane. The combined organic layers were dried over Na.sub.2SO.sub.4, and concentrated to give Compound AN (yield 450 mg, 60%).

(155) Step 8:

(156) To a solution of Compound AN (600 mg, 1.76 mmol) in dichloromethane (8 mL) was added TFA (2 mL) at 0 C. After the reaction mixture was stirred for 2 hours at room temperature, it was concentrated to give Compound AG without further purification.

Example 31

2-[7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isopropyl-3-oxo-1,5,6,8-tetrahydroimidazo[1,5-a]pyrazin-8a-yl]acetic Acid

(157) ##STR00073##

(158) The title compound was prepared in analogy to Example 30 by using 2-(2-isopropyl-3-oxo-5,6,7,8-tetrahydro-1H-imidazo[1,5-a]pyrazin-8a-yl)acetic acid (Compound AO) instead of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]benzoic acid (Compound AG). Example 31 was obtained as a light yellow solid (8 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.94 (d, J=3.0 Hz, 1H), 7.73 (d, J=3.3 Hz, 1H), 7.43 (dd, J=6.0, 8.8 Hz, 1H), 7.24 (dd, J=2.8, 8.8 Hz, 1H), 7.11-7.02 (m, 1H), 6.16 (s, 1H), 4.18-4.04 (m, 2H), 3.79 (d, J=16.6 Hz, 2H), 3.61 (s, 3H), 3.47 (d, J=9.5 Hz, 1H), 3.29-3.24 (m, 1H), 3.20 (d, J=9.5 Hz, 1H), 3.09 (d, J=11.5 Hz, 1H), 2.90 (d, J=15.6 Hz, 2H), 2.38-2.12 (m, 3H), 1.18 (d, J=7.0 Hz, 3H), 1.12 (d, J=6.8 Hz, 3H). MS: calc'd 606 (MH.sup.+), measured 606 (MH.sup.+).

Preparation of 2-(2-isopropyl-3-oxo-5,6,7,8-tetrahydro-1H-imidazo[1,5-a]pyrazin-8a-yl)acetic Acid (Compound AO)

(159) The title compound was prepared in analogy to Compound AG in Example 30 by using iso-propyl amine instead of cyclopropyl amine.

Example 32

(R)-6-[(S)-2-(1-Carboxy-1-methyl-ethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(160) ##STR00074##

(161) The title compound was prepared in analogy to Example 19 by using methyl 2-amino-2-methyl-propanoate hydrochloride salt instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 32 was obtained as a light yellow solid (60 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98 (d, 1H), 7.79 (d, 1H), 7.23-7.38 (m, 2H), 7.09-7.23 (m, 1H), 6.25 (s, 1H), 4.23 (br. s., 1H), 4.06 (m, 3H), 3.78-3.97 (m, 2H), 3.59-3.71 (m, 1H), 3.14-3.29 (m, 2H), 2.89-3.14 (m, 2H), 2.54 (br. s., 2H), 1.40-1.57 (m, 6H), 1.04-1.19 (m, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Example 33

3-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-3-methyl-butanoic Acid

(162) ##STR00075##

(163) The title compound was prepared in analogy to Example 19 by using 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3-methyl-butanoic acid (Compound AP) instead of 2-chloro-3-fluorobenzaldehyde, ethyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q). Example 33 was obtained as a light yellow solid (381 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.90-7.91 (d, J=4.0 Hz, 1H), 7.55 (m, 1H), 7.29 (m, 1H), 7.13 (m, 1H), 6.20 (s, 1H), 4.76 (d, J=16.0 Hz, 1H), 4.60 (d, J=16.0 Hz, 1H), 4.12 (m, 1H), 4.02-4.06 (m, 1H), 3.70-3.80 (m, 3H), 3.65 (s, 3H), 3.32-3.44 (m, 2H), 3.20 (m, 2H), 3.09-3.05 (d, J=16.0 Hz, 1H), 2.79-2.83 (d, J=16.0 Hz, 1H), 1.47 (s, 6H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3-methyl-butanoic Acid (Compound AP)

(164) The title compound was prepared in analogy to Compound Q in Example 19 by using methyl 3-amino-3-methyl-butanoate hydrochloride salt (Compound AP-1) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of Methyl 3-amino-3-methyl-butanoate Hydrochloride Salt (Compound AP-1)

(165) ##STR00076##

(166) Compound AP-1 was prepared in analogy to Compound W in Example 20 by using 3-amino-3-methyl-butyric acid instead of DL-3-aminoisobutyric acid.

Example 34

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-3-methyl-Butanoic Acid

(167) ##STR00077##

(168) The title compound was prepared in analogy to Example 19 by using 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3-methyl-butanoic acid (Compound AP) instead of 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q). Example 34 was obtained as a light yellow solid (26 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.99-8.00 (d, J=4.0 Hz, 1H), 7.83-7.84 (d, J=4.0 Hz, 1H), 7.35 (m, 2H), 7.20 (m, 1H), 6.25 (s, 1H), 4.20-4.40 (m, 2H), 4.10 (m, 2H), 3.94 (m, 2H), 3.70 (m, 2H), 3.29 (m, 5H), 3.02-3.06 (d, J=16.0 Hz, 1H), 2.80-2.84 (d, J=16.0 Hz, 1H), 1.46 (s, 6H), 1.11-1.15 (t, J.sub.1=8.0 Hz, J.sub.2=16.0 Hz, 3H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Example 35

1-[[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]cyclopropanecarboxylic Acid

(169) ##STR00078##

(170) The title compound was prepared in analogy to Example 19 by using 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and methyl 1-(aminomethyl)cyclopropanecarboxylate hydrochloride salt (Compound AQ) instead of 2-chloro,3-fluorobenzaldehyde, ethyl acetoacetate and ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 35 was obtained as a light yellow solid (760 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.90-7.91 (d, J=4.0 Hz, 1H), 7.55 (m, 1H), 7.29 (m, 1H), 7.13 (m, 1H), 6.20 (s, 1H), 4.77 (d, J=16.0 Hz, 1H), 4.59 (d, J=16.0 Hz, 1H), 4.19 (m, 1H), 4.07-4.10 (m, 1H), 3.73-3.77 (m, 3H), 3.65 (s, 3H), 3.36-3.50 (m, 4H), 3.15-3.24 (m, 2H), 1.29 (m, 2H), 1.02 (m, 2H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Preparation of Methyl 1-(aminomethyl)cyclopropanecarboxylate Hydrochloride Salt (Compound AQ)

(171) The title compound was prepared in analogy to Compound W in Example 20 by using 1-(aminomethyl)cyclopropanecarboxylic acid (CAS number: 139132-50-6, J&K; for its synthesis, please refer to: Mertin A., et al. Synlett, 1991, 2, 87-9) instead of DL-3-aminoisobutyric acid.

Example 36

1-[[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]cyclopropanecarboxylic Acid

(172) ##STR00079##

(173) The title compound was prepared in analogy to Example 19 by using methyl 1-(aminomethyl)cyclopropanecarboxylate hydrochloride salt (Compound AQ) instead ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt. Example 36 was obtained as a light yellow solid (170 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.92-7.91 (d, J=4.0 Hz, 1H), 7.38 (m, 2H), 7.26 (m, 1H), 6.27 (s, 1H), 4.77 (d, J=16.0 Hz, 1H), 4.59 (d, J=16.0 Hz, 1H), 4.11 (m, 1H), 4.07-4.10 (m, 3H), 3.72-3.78 (m, 3H), 3.36-3.47 (m, 4H), 3.15-3.24 (m, 2H), 1.29 (m, 2H), 1.13 (t, J.sub.1=8.0 Hz, J.sub.2=16.0 Hz, 3H), 1.02 (m, 2H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Example 37

1-[[(8aS)-7-[[(4S)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]cyclopropanecarboxylic Acid

(174) ##STR00080##

(175) The title compound was prepared in analogy to Example 19 by using 2-methyl-3,4-difluorobenzaldehyde, methyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3-methyl-butanoic acid (Compound AP) instead of 2-chloro-3-fluorobenzaldehyde, ethyl acetoacetate and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q). Example 37 was obtained as a light yellow solid (153 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.90-7.91 (d, J=4.0 Hz, 1H), 7.26 (m, 1H), 7.10 (m, 1H), 5.95 (s, 1H), 4.74 (d, J=16.0 Hz, 1H), 4.57 (d, J=16.0 Hz, 1H), 4.10 (m, 2H), 3.70-3.80 (m, 3H), 3.66 (s, 3H), 3.36-3.42 (m, 2H), 3.20 (m, 2H), 3.09-3.05 (d, J=16.0 Hz, 1H), 2.79-2.83 (d, J=16.0 Hz, 1H), 2.53 (s, 3H), 1.47 (s, 6H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Example 38

1-[[(8aS)-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]cyclopropanecarboxylic Acid

(176) ##STR00081##

(177) The title compound was prepared in analogy to Example 35 by using 2-methyl-3,4-difluorobenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde. Example 38 was obtained as a light yellow solid (54 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.90-7.91 (d, J=4.0 Hz, 1H), 7.26 (m, 1H), 7.12 (m, 1H), 5.95 (s, 1H), 4.74 (d, J=16.0 Hz, 1H), 4.56 (d, J=16.0 Hz, 1H), 4.07-4.17 (m, 2H), 3.70-3.77 (m, 3H), 3.66 (s, 3H), 3.35-3.46 (m, 4H), 3.15-3.22 (m, 2H), 2.51 (s, 3H), 1.29 (m, 2H), 1.02 (m, 2H). MS: calc'd 601 (MH.sup.+), measured 601 (MH.sup.+).

Example 39

3-[(2S,8aR)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclobutanecarboxylic Acid

(178) ##STR00082##

(179) The title compound was prepared in analogy to Example 19 by using 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and methyl trans-3-amino-cyclobutanecarboxylate hydrochloride (CAS number: 74316-29-3; for its synthesis, please refer to: Grygorenko O. O., et al. Synthetic Communications, 2011, 41, 1644-1649) instead of 2-chloro-3-fluorobenzaldehyde, ethyl acetoacetate and ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride. Example 39 was obtained as a light yellow solid (44 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (dd, J=4.0, 3.3 Hz, 1H), 7.77 (dd, J=3.1, 1.4 Hz, 1H), 7.43 (ddd, J=8.7, 6.1, 2.5 Hz, 1H), 7.20-7.28 (m, 1H), 6.99-7.13 (m, 1H), 6.18 (s, 1H), 4.58-4.73 (m, 1H), 4.11 (dd, J=16.9, 4.6 Hz, 1H), 3.73-3.97 (m, 3H), 3.61 (s, 3H), 2.82-3.28 (m, 5H), 2.74 (d, J=11.8 Hz, 1H), 2.15-2.65 ppm (m, 6H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Example 40

3-[(8aR)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclobutanecarboxylic Acid

(180) ##STR00083##

(181) The title compound was prepared in analogy to Example 19 by using 2-chloro-4-fluorobenzaldehyde, methyl acetoacetate and methyl cis-3-amino-cyclobutanecarboxylate hydrochloride (CAS number: 1212304-86-3; for its synthesis, please refer to: Grygorenko O. O., et al. Synthetic Communications, 2011, 41, 1644-1649) instead of 2-chloro-3-fluorobenzaldehyde, ethyl acetoacetate and ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride. Example 40 was obtained as a light yellow solid (5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.92-8.02 (m, 1H), 7.77 (dd, J=3.1, 1.4 Hz, 1H), 7.38-7.48 (m, 1H), 7.24 (d, J=6.3 Hz, 1H), 6.98-7.15 (m, 1H), 6.18 (s, 1H), 4.58-4.75 (m, 1H), 4.04-4.19 (m, 1H), 3.72-3.97 (m, 3H), 3.61 (s, 4H), 2.81-3.27 (m, 6H), 2.33-2.63 ppm (m, 5H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Example 41

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(182) ##STR00084##

(183) The title compound was prepared in analogy to Example 19 by using methyl acetoacetate instead of ethyl acetoacetate. Example 41 was obtained as a light yellow solid (250 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.09-7.36 (m, 3H), 6.23 (s, 1H), 4.04-4.15 (m, 1H), 3.78-3.98 (m, 3H), 3.61 (s, 3H), 3.36-3.55 (m, 3H), 3.04-3.28 (m, 2H), 2.76-2.99 (m, 2H), 2.12-2.45 (m, 2H), 1.15-1.25 ppm (m, 6H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Example 42

3-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(184) ##STR00085##

(185) The title compound was prepared in analogy to Example 19 by using 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate instead of 2-chloro-3-fluorobenzaldehyde and ethyl acetoacetate. Example 42 was obtained as a light yellow solid (260 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.42 (dd, J=8.5, 6.0 Hz, 1H), 7.24 (dd, J=8.7, 2.6 Hz, 1H), 7.06 (td, J=8.4, 2.8 Hz, 1H), 6.17 (s, 1H), 4.09 (d, J=16.8 Hz, 1H), 3.79-3.98 (m, 3H), 3.61 (s, 3H), 3.51 (t, J=8.9 Hz, 1H), 3.36-3.44 (m, 1H), 3.04-3.26 (m, 3H), 2.75-3.00 (m, 2H), 2.11-2.43 (m, 2H), 1.20 ppm (d, J=3.0 Hz, 6H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Example 43

3-[(8aS)-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(186) ##STR00086##

(187) The title compound was prepared in analogy to Example 19 by using 2-methyl-3,4-difluorobenzaldehyde and methyl acetoacetate instead of 2-chloro-3-fluorobenzaldehyde and ethyl acetoacetate. Example 43 was obtained as a light yellow solid (110 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.3 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 6.96-7.09 (m, 2H), 5.93 (s, 1H), 4.04-4.16 (m, 1H), 3.78-3.98 (m, 3H), 3.62 (s, 3H), 3.36-3.56 (m, 3H), 3.05-3.26 (m, 2H), 2.74-2.97 (m, 2H), 2.57 (d, J=2.3 Hz, 3H), 2.36 (td, J=11.7, 3.6 Hz, 1H), 2.18 (t, J=10.9 Hz, 1H), 1.20 ppm (d, J=3.3 Hz, 6H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Example 44

3-[(8aS)-7-[[(4S)-4-(3-fluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(188) ##STR00087##

(189) The title compound was prepared in analogy to Example 19 by using 2-methyl-3-fluorobenzaldehyde and methyl acetoacetate instead of 2-chloro-3-fluorobenzaldehyde and ethyl acetoacetate. Example 44 was obtained as a light yellow solid (74 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.0 Hz, 1H), 7.74 (d, J=3.3 Hz, 1H), 7.03-7.21 (m, 2H), 6.88-6.99 (m, 1H), 5.98 (s, 1H), 4.03-4.17 (m, 1H), 3.79-3.99 (m, 4H), 3.62 (s, 3H), 3.41-3.56 (m, 2H), 3.07-3.26 (m, 2H), 2.75-2.98 (m, 2H), 2.53 (d, J=2.0 Hz, 3H), 2.29-2.45 (m, 1H), 2.18 (t, J=11.2 Hz, 1H), 1.20 ppm (d, J=3.3 Hz, 6H). MS: calc'd 585 (MH.sup.+), measured 585 (MH.sup.+).

Example 45

7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(190) ##STR00088##

Preparation of Example 45

(191) ##STR00089##

(192) The solution of methyl 7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylate (Compound AQ, 150 mg, 0.25 mmol) in tetrahydrofuran (1.5 mL) was added lithium hydroxide monohydrate (52 mg, 1.25 mmol) in water (1.5 mL). After the reaction mixture was stirred at room temperature for 2 hours, it was neutralized by 1N hydrochloride solution to pH 3.0. The mixture was extracted with ethyl acetate (30 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and then concentrated. The residue was purified by Prep-HPLC to give Example 45 as a mixture of two diastereomers (5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.06-7.95 (m, 1H), 7.87 (s, 1H), 7.55-7.44 (m, 1H), 7.32-7.23 (m, 1H), 7.15-7.03 (m, 1H), 6.18 (s, 1H), 4.37-4.21 (m, 1H), 4.09-3.91 (m, 1H), 3.91-3.77 (m, 2H), 3.62 (d, J=2.0 Hz, 4H), 3.52-3.39 (m, 2H), 3.22-2.96 (m, 2H), 2.75-2.58 (m, 1H), 2.50-2.42 (m, 1H), 0.74 (d, J=1.5 Hz, 4H). MS: calc'd 589 (MH.sup.+), measured 589 (MH.sup.+).

Preparation of 7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylate (Compound AQ)

(193) The title compound was prepared in analogy to Example 1 by using methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound AR) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D).

Preparation of cis-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound AR)

(194) ##STR00090## ##STR00091## ##STR00092##
Step 1:

(195) To a solution of dimethyl maleate (7.2 g, 50 mmol) in tetrachloromethane (150 mL) was added dropwise bromine (8.8 g, 55 mmol) in tetrachloromethane (10 mL) at 0 C. under nitrogen atmosphere. The reaction mixture was stirred at 0 C. overnight. The reaction mixture was quenched by adding ice-water, and then washed with Na.sub.2SO.sub.3 solution. The organic phase was washed with water, separated, dried over Na.sub.2SO.sub.4 and then concentrated to give crude Compound AS (15.2 g, crude).

(196) Step 2:

(197) To a solution of Compound AS (10.6 g, 35 mmol) in benzene (120 mL) was added dropwise N,N-dibenzylethylenediamine (8.4 g, 35 mmol) and triethyl amine (9.7, 70 mmol) in benzene (20 mL) at 40 C. under nitrogen atmosphere. The reaction mixture was stirred for 10 minutes and heated to reflux overnight. The reaction mixture was cooled down by adding ice-water, and then extracted with petroleum ether/ethyl acetate=10/1 (50 mL) three times, the combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and then concentrated. The residue was purified by column chromatography to give Compound AT (3.3 g).

(198) Step 3:

(199) To a solution of Compound AT (4.5 g, 11.8 mmol) in THF (60 mL) was added di-tert-butyl dicarbonate and palladium hydroxide on carbon (1.0 g). The reaction mixture was heated to 45 C. overnight under pressure hydrogen atmosphere. The reaction mixture was filtrated and concentrated. The residue was purified by column chromatography to give Compound AU (3.6 g).

(200) Step 4:

(201) To a solution of Compound AU (760 mg, 1.89 mmol) in methanol (4.0 mL) at 55 C. was added dropwise sodium hydroxide (98 mg, 2.46 mmol) in water (1 mL). The reaction mixture was stirred at 55 C. for 2 hours. The reaction mixture was cooled down and neutralized to pH 3.0, and then extracted with ethyl acetate (30 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and then concentrated to give crude Compound AV (660 mg).

(202) Step 5:

(203) To a solution of Compound AV (776 mg, 2.0 mmol) in dichloromethane (8.0 mL) was added cyclopropyl amine (120 mg, 2.0 mmol), HATU (950 mg, 2.5 mmol) and diisopropylethylamine (0.5 mL). The reaction mixture was stirred at room temperature overnight. The reaction mixture was quenched by adding ice-water, and then extracted with dichloromethane (30 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, and then filtrated and concentrated to give crude Compound AW (578 mg).

(204) Step 6:

(205) To a mixture of phenylsilane (1.5 mL) and [Ir(COD).sub.2Cl].sub.2 (7 mg, 0.01 mmol) in dichloromethane (1.5 mL) was added Compound AW (128 mg, 2.0 mmol). The reaction mixture was stirred at 40 C. overnight. The reaction mixture was concentrated and then diluted with dichloromethane (20 mL), then quenched by adding 3 drops of hydrochloride in dioxane solution (3N). The reaction mixture was washed with water. The organic phase was separated and dried over Na.sub.2SO.sub.4, and then filtrated and concentrated. The residue was purified by column chromatography to give crude Compound AX (70 mg).

(206) Step 7:

(207) To a solution of Compound AX (72 mg, 0.174 mmol) in dichloromethane (2.0 mL) was added 4-nitrophenyl chloroformate (70 mg, 0.35 mmol) and diisopropylethylamine (5 drops). After the reaction mixture was heated to 40 C. for 2 hours, it was concentrated and the residue was purified by column chromatography to give Compound AY (81 mg).

(208) Step 8:

(209) The mixture of Compound AY (578 mg, 1.0 mmol) and TFA/DCM=2/1 (9 mL) was stirred at room temperature for 2 hours. The reaction mixture was concentrated and added toluene for co-evaporation to remove trifluoroacetic acid. The residue was dissolved in dichloromethane (8.0 mL), and then diisopropylethylamine (2 mL) was added. The reaction mixture was heated to 40 C. for 3 hours. The reaction mixture was concentrated to give Compound AR (239 mg, crude).

Example 46

2-[1-[(8aR)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopropyl]acetic Acid

(210) ##STR00093##

Preparation of Example 46

(211) ##STR00094##

(212) The title compound was prepared in analogy to Example 1 by using 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid (Compound 46-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 46 was obtained as a light yellow solid (65 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.42 (dd, J=6.1, 8.7 Hz, 1H), 7.24 (dd, J=2.8, 8.8 Hz, 1H), 7.05 (dt, J=2.5, 8.4 Hz, 1H), 6.17 (s, 1H), 4.15-4.02 (m, 1H), 3.96-3.71 (m, 3H), 3.66-3.53 (m, 4H), 3.22-3.07 (m, 2H), 2.90 (d, J=11.0 Hz, 1H), 2.80 (d, J=9.0 Hz, 1H), 2.68-2.56 (m, 1H), 2.55-2.46 (m, 1H), 2.37 (dt, J=3.1, 11.6 Hz, 1H), 2.18 (t, J=10.9 Hz, 1H), 1.03-0.81 (m, 4H), MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Preparation of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic Acid (Compound 46-A)

(213) ##STR00095##
Step 1:

(214) tert-Butyl (3S)-3-formyl-4-methyl-piperazine-1-carboxylate (Compound S, 346 mg, 1 mmol) in dichloromethane (3 mL) and Et.sub.3N (0.5 mL) was added 4-aminobutanoic acid (103 mg, 1 mmol). The reaction mixture was stirred for 1 hour at room temperature, then concentrated under reduced pressure. Then methanol (5 mL) and sodium cyanoborohydride (248 mg, 4 mmol) was added and the reaction mixture was stirred for another 3 hours at room temperature. The mixture was concentrated under reduced pressure to give crude compound 46-B.

(215) Step 2:

(216) Compound 46-B in THF (10 mL) was added potassium tert-butoxide (224 mg, 2 mmol), the reaction mixture was stirred at 80 C. for 4 h. The solution was cooled to room temperature and acidified to pH 5 with aqueous HCl solution. The mixture was then extracted with ethyl acetate 3 times. The combined organic layers were washed with brine, dried over Na.sub.2SO.sub.4, and concentrated under reduced pressure to give crude compound 46-C (301 mg).

(217) Step 3:

(218) To a stirred solution of compound 46-C (301 mg, 0.92 mmol) was dissolved in dichloromethane (4 mL) and treated with trifluoroacetic acid (2 mL) at room temperature. After the reaction mixture was stirred at room temperature for 1 hour, the solvent was removed in vacuo to give 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid (Compound 46-A) which was used directly.

Example 47

2-[1-[(8aR)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopropyl]acetic Acid

(219) ##STR00096##

Preparation of Example 47

(220) ##STR00097##

(221) The title compound was prepared in analogy to Example 1 by using 2-[1-[(8aR)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2yl]cyclopropyl]acetic acid (Compound 47-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 47 was obtained as a light yellow solid (62 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.42 (dd, J=6.1, 8.7 Hz, 1H), 7.24 (dd, J=2.8, 8.8 Hz, 1H), 7.05 (dt, J=2.5, 8.4 Hz, 1H), 6.17 (s, 1H), 4.15-4.02 (m, 1H), 3.96-3.71 (m, 3H), 3.66-3.53 (m, 4H), 3.22-3.07 (m, 2H), 2.90 (d, J=11.0 Hz, 1H), 2.80 (d, J=9.0 Hz, 1H), 2.68-2.56 (m, 1H), 2.55-2.46 (m, 1H), 2.37 (dt, J=3.1, 11.6 Hz, 1H), 2.18 (t, J=10.9 Hz, 1H), 1.03-0.81 (m, 4H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Preparation of 2-[1-[(8aR)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2yl]cyclopropyl]acetic Acid (Compound 47-A)

(222) Compound 47-A was prepared in analogy to compound Q in Example 19 by using methyl 2-(1, -aminocyclopropyl)acetate hydrochloride salt (for its synthesis, refer to: Sandstroem A., et al, Bioorganic & Medicinal Chemistry, 16(10), 5590-5605; 2008) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 48

2-[1-[(8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopropyl]acetic Acid

(223) ##STR00098##

Preparation of Example 48

(224) ##STR00099##

(225) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 2-[1-[(8aR)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2yl]cyclopropyl]acetic acid (Compound 47-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 48 was obtained as a light yellow solid (76 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (s, 1H), 7.76 (s, 1H), 7.37-7.23 (m, 2H), 7.21-7.10 (m, 1H), 6.24 (s, 1H), 4.04 (d, J=7.0 Hz, 3H), 3.95-3.77 (m, 3H), 3.66-3.54 (m, 1H), 3.20-3.08 (m, 2H), 2.95-2.86 (m, 1H), 2.86-2.74 (m, 1H), 2.67-2.57 (m, 1H), 2.55-2.45 (m, 1H), 2.42-2.30 (m, 1H), 2.23-2.12 (m, 1H), 1.13 (t, J=7.2 Hz, 3H), 1.01-0.81 (m, 4H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of Ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-1)

(226) Compound C-1 was prepared in analogy to compound C by using ethyl acetoacetate and 2-chloro-3-fluorobenzaldehyde instead of methyl acetoacetate and 2-chloro-4-fluorobenzaldehyde.

Example 49

(1S,2R)-2-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid

(227) ##STR00100##

Preparation of Example 49

(228) ##STR00101##

(229) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and (1R,2R)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid (Compound 49-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 49 was obtained as a light yellow solid (48 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.27 (s, 2H), 7.21-7.12 (m, 1H), 6.24 (s, 1H), 4.47-4.38 (m, 1H), 4.17-3.98 (m, 3H), 3.97-3.86 (m, 2H), 3.83-3.75 (m, 1H), 3.59 (s, 1H), 3.25-3.11 (m, 2H), 3.03-2.94 (m, 1H), 2.93-2.82 (m, 1H), 2.77-2.68 (m, 1H), 2.45-2.35 (m, 1H), 2.29-2.17 (m, 1H), 2.11-1.84 (m, 3H), 1.82-1.68 (m, 3H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Preparation of (1R,2R)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid (Compound 49-A)

(230) Compound 49-A was prepared in analogy to compound Q in Example 19 by using ethyl (1R,2R)-2-aminocyclopentanecarboxylate (Accela Chembio Co., Ltd, SY024586) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 50

(1R,2R)-2-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid

(231) ##STR00102##

Preparation of Example 50

(232) ##STR00103##

(233) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and (1S,2R)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid (Compound 50-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 50 was obtained as a light yellow solid (30 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 6.97-7.39 (m, 2H), 6.24 (s, 1H), 4.42 (d, J=9.8 Hz, 2H), 3.73-4.19 (m, 5H), 3.52 (m, 4H), 3.12-3.23 (m, 2H), 2.66-2.96 (m, 2H), 2.11-2.47 (m, 3H), 1.60-2.05 (m, 4H), 1.13 ppm (t, J=7.2 Hz, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Preparation of (1S,2R)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid (Compound 50-A)

(234) Compound 50-A was prepared in analogy to compound Q in Example 19 by using ethyl (1S,2R)-2-aminocyclopentanecarboxylate trifluoroacetic acid salt (compound 50-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of Ethyl (1S,2R)-2-aminocyclopentanecarboxylate Trifluoroacetic Acid Salt (Compound 50-B)

(235) ##STR00104##

(236) (1R,2S)-2-(Boc-amino)cyclopentanecarboxylate (CAS: 1140972-29-7, TCI) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. The reaction mixture was stirred at rt for 1 hour and then the solvent was removed under vacuum to give the crude product 50-B, which was used directly in the next step.

Example 51

(1R,2S)-2-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxyli

(237) ##STR00105##

Preparation of Example 51

(238) ##STR00106##

(239) The title compound was prepared in analogy to Example 1 by using (1R,2S)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 51-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 51 was obtained as a light yellow solid (68 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.07-7.89 (m, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.44 (t, J=6.9 Hz, 1H), 7.25 (dd, J=2.5, 8.8 Hz, 1H), 7.06 (dt, J=2.5, 8.4 Hz, 1H), 6.18 (s, 1H), 4.41 (q, J=8.6 Hz, 1H), 4.19 (d, J=16.8 Hz, 1H), 4.05-3.79 (m, 3H), 3.64-3.57 (m, 4H), 3.27-3.14 (m, 1H), 3.10 (dd, J=4.3, 9.0 Hz, 1H), 2.99 (br. s., 1H), 2.94-2.77 (m, 2H), 2.48 (br. s., 1H), 2.29 (br. s., 1H), 2.08-1.84 (m, 3H), 1.84-1.69 (m, 3H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of (1R,2S)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid TFA Salt (Compound 51-A)

(240) Compound 51-A was prepared in analogy to compound Q in Example 19 by using ethyl (1R,2S)-2-aminocyclopentanecarboxylate (Accela Chembio Co., Ltd, CAS: 197916-36-2) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 52

(1S,2S)-2-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid

(241) ##STR00107##

Preparation of Example 52

(242) ##STR00108##

(243) The title compound was prepared in analogy to Example 1 by using (1S,2S)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 52-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 52 was obtained as a light yellow solid (1.7 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 1H NMR (MeOD, 400 MHz): d=7.97 (d, J=3.0 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.42 (dd, J=6.0, 8.8 Hz, 1H), 7.24 (dd, J=2.8, 8.8 Hz, 1H), 7.06 (dt, J=2.8, 8.4 Hz, 1H), 6.18 (s, 1H), 4.50-4.25 (m, 1H), 4.09 (d, J=17.1 Hz, 1H), 3.98-3.72 (m, 3H), 3.61 (s, 3H), 3.52 (t, J=8.9 Hz, 1H), 3.26-3.01 (m, 2H), 2.96-2.72 (m, 3H), 2.41-2.17 (m, 2H), 2.05-1.84 (m, 3H), 1.81-1.67 (m, 3H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of (1S,2S)-2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid TFA Salt (Compound 52-A)

(244) Compound 52-A was prepared in analogy to compound Q in Example 19 by using ethyl (1S,2S)-2-aminocyclopentanecarboxylate (Accela Chembio Co., Ltd, CAS: 752181-59-2) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 53

4-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]butanoic Acid

(245) ##STR00109##

Preparation of Example 53

(246) ##STR00110##

(247) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid (Compound 46-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 53 was obtained as a light yellow solid (2 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.27 (s, 2H), 7.16 (s, 1H), 6.24 (s, 1H), 4.18-4.01 (m, 3H), 3.92 (d, J=16.8 Hz, 3H), 3.49 (s, 1H), 3.30-3.25 (m, 1H), 3.25-3.15 (m, 2H), 3.12-3.04 (m, 1H), 2.97-2.81 (m, 2H), 2.33 (s, 3H), 2.26-2.14 (m, 1H), 1.85 (d, J=7.0 Hz, 2H), 1.13 (t J=7.2 Hz, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Example 54

4-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-butanoic Acid

(248) ##STR00111##

Preparation of Example 54

(249) ##STR00112##

(250) The title compound was prepared in analogy to Example 1 by using 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-butanoic acid TFA salt (Compound 54-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 54 was obtained as a light yellow solid (23 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01 (d, J=3.0 Hz, 1H), 7.90 (d, J=3.0 Hz, 1H), 7.53 (dd, J=5.9, 8.7 Hz, 1H), 7.29 (dd, J=2.6, 8.7 Hz, 1H), 7.17-7.06 (m, 1H), 6.20 (s, 1H), 4.77-4.64 (m, 1H), 4.57-4.42 (m, 1H), 4.27-4.12 (m, 1H), 4.07 (dd, J=3.3, 14.8 Hz, 1H), 3.75-3.62 (m, 5H), 3.58 (t, J=9.0 Hz, 1H), 3.51-3.37 (m, 2H), 3.27-3.02 (m, 4H), 1.95-1.82 (m, 1H), 1.80-1.66 (m, 1H), 1.31-1.19 (m, 6H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Preparation of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-butanoic Acid TFA Salt (Compound 54-A)

(251) ##STR00113##
Step 1:

(252) A mixture of 2,2-dimethylbutyrolactone (6.84 g, 60 mmol) and KOH (3.36 g) in H.sub.2O (60 mL) was heated at reflux for 2 h. The solution was cooled to room temperature and acidified to pH 5 with aqueous HCl solution. The mixture was then extracted with ethyl acetate 3 times. The combined organic layers were washed with brine, dried over Na.sub.2SO.sub.4, concentrated under reduced pressure to give 4-hydroxy-2,2-dimethyl-butanoic acid compound 54-B (4 g).

(253) Step 2:

(254) To the mixture of compound 54-B (2.2 g, 16.6 mmol) in ethyl ether (16 mL) and methanol (24 mL) at 0 C. was added a hexane solution (2.0 M) of trimethylsilyldiazomethane (12.5 mL, 25 mmol). The reaction mixture was stirred at 0 C. for 1 h. The solvent was evaporated and the residue was taken up in ethyl acetate, washed successively with diluted aqueous HCl solution, saturated NaHCO.sub.3 solution and brine. The mixture was dried over Na.sub.2SO.sub.4 and concentrated to give 4-hydroxy-2,2-dimethyl-butyric acid methyl ester compound 54-C (1.5 g).

(255) Step 3:

(256) To a solution of the alcohol 54-C (45 mg, 0.34 mmol) in THF (4 mL) at 0 C. were added Ph.sub.3P (136 mg, 0.52 mmol), imidazole (71 mg, 1.04 mmol), and I.sub.2 (132 mg, 0.52 mmol). After 1 h, the reaction mixture was quenched with saturated Na.sub.2S.sub.2O.sub.3 solution. The aqueous layer was extracted twice with hexanes. The organic layer were dried over Na.sub.2SO.sub.4 and concentrated under vacuum to give compound 54-D as a crude product.

(257) Step 4:

(258) To a stirred solution of compound M (256 mg, 1 mmol) in DMF (2 mL) was added NaH (48 mg, 2 mmol) at room temperature. The reaction mixture was stirred 20 min at room temperature, then compound 54-D (256 mg, 1 mmol) was added. After the reaction mixture was stirred for 4 hours at room temperature, EA was added and the mixture was washed with water and brine. The mixture was dried over Na.sub.2SO.sub.4, and concentrated under reduced pressure to give the crude product, which was purified by column to give compound 54-E (150 mg).

(259) Step 5:

(260) To a stirred solution of compound 54-E (150 mg, 0.4 mmol) in THF (5 mL) and water (2 mL) was added LiOH (96 mg 2.4 mmol). After the reaction mixture was stirred at 80 C. for 18 h, it was concentrated under reduced pressure to give the crude product, which was dissolved in DCM (4 mL) and treated with TFA (4 mL). The mixture was stirred for 2 hours at room temperature, then it was concentrated under reduced pressure to give the crude product 54-A which was used directly.

Example 55

4-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-3,3-dimethyl-butanoic Acid

(261) ##STR00114##

Preparation of Example 55

(262) ##STR00115##

(263) The title compound was prepared in analogy to Example 1 by using 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3,3-dimethyl-butanoic acid TFA salt (Compound 55-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 55 was obtained as a light yellow solid (22 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.50-7.35 (m, 1H), 7.29-7.20 (m, 1H), 7.12-7.01 (m, 1H), 6.18 (s, 1H), 4.20-4.06 (m, 1H), 3.94 (s, 3H), 3.61 (s, 4H), 3.19 (d, J=14.6 Hz, 3H), 3.08-2.99 (m, 1H), 2.97-2.82 (m, 2H), 2.47-2.34 (m, 1H), 2.27 (s, 3H), 1.07 (s, 6H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Preparation of 4-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-3,3-dimethyl-butanoic Acid TFA Salt (Compound 55-A)

(264) ##STR00116##
Step 1:

(265) 4,4-Dimethyl-2-pyrrolidinone (2.52 g, 22.3 mmol) was added to a mixture of concentrated HCl (50 mL) and water (50 mL) and the resulting mixture was refluxed at 120 C. for 20 hours. After it was cooled to room temperature, the mixture was washed twice with dichloromethane. The aqueous layer was evaporated to give 4-amino-3,3-dimethyl-butyric acid hydrochloride compound 55-B (3.4 g) as a white solid.

(266) Step 2:

(267) Aldehyde S (346 mg, 1 mmol) in dichloromethane (3 mL) and Et.sub.3N (0.5 mL) was added compound 55-B (131 mg, 1 mmol). The reaction mixture was stirred for 1 hour at room temperature, then concentrated under reduced pressure. Methanol (5 mL) was added followed by the addition of sodium cyanoborohydride (248 mg, 4 mmol). After the reaction mixture was stirred at room temperature for 1 h, it was concentrated under reduced pressure to give crude product 55-C.

(268) Step 3:

(269) Compound 55-C in THF (10 mL) was added potassium tert-butoxide (224 mg, 2 mmol), then the reaction mixture was stirred at 80 C. for 4 h. The solution was cooled to room temperature and acidified to pH 5 with aqueous HCl solution. The mixture was then extracted with ethyl acetate 3 times. The combined organic layers were washed with brine, dried over Na.sub.2SO.sub.4, and concentrated under reduced pressure to give crude product 55-D.

(270) Step 4:

(271) To a stirred solution of compound 55-D (301 mg, 0.92 mmol) in dichloromethane (4 mL) was added trifluoroacetic acid (2 mL) at room temperature. After the reaction mixture was stirred at room temperature for 1 hour, the solvent was removed under vacuum to give crude product 55-A which was used directly.

Example 56

(R)-6-[(S)-2-(2-Carboxy-ethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(272) ##STR00117##

Preparation of Example 56

(273) ##STR00118##

(274) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]propanoic acid TFA salt (Compound 56-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 56 was obtained as a light yellow solid (60 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95-8.00 (m, 1H), 7.79 (d, 1H), 7.25-7.36 (m, 2H), 7.12-7.22 (m, 1H), 6.25 (s, 1H), 4.24 (d, 1H), 4.06 (m, 3H), 3.86-4.00 (m, 2H), 3.50-3.62 (m, 2H), 3.36-3.50 (m, 1H), 3.24 (m, 1H), 3.15 (m, 1H), 3.04 (d, 2H), 2.56 (m, 3H), 2.40 (br. s., 1H), 1.13 (m, 3H). MS: calc'd 591 (MH.sup.+), measured 591 (MH.sup.+).

Preparation of 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]propanoic Acid TFA Salt (Compound 56-A)

(275) ##STR00119##
Step 1.

(276) To a stirred solution of compound M (150 mg, 0.62 mmol) in THF (3 mL) was added methyl acrylate (534 mg, 6.20 mmol) at room temperature, followed by a small amount of NaOH as a catalyst. LC-MS indicated compound M was consumed completely and the product 56-B was formed already. The resulting mixture was stirred at rt for 6 hours. The reaction mixture was then concentrated and directly used in the next step without further purification. The crude product amount was 180 mg.

(277) Step 2.

(278) To a solution of compound 56-B (180 mg, 0.55 mmol) in THF (3 mL) was added a solution of lithium hydroxide monohydrate (116 mg, 2.75 mmol) in H.sub.2O (1 mL) at room temperature. The resulting mixture was stirred at room temperature for 3 hours. LC-MS showed starting material 56-B was consumed completely. The mixture was adjusted to pH 4-5 with 1 N HCl, then concentrated. The residue was used directly in the next step and the amount of crude product 56-C was 300 mg.

(279) Step 3.

(280) To a solution of 56-C (crude 300 mg, 0.55 mmol) in dichloromethane (3 mL) was added trifluoroacetic acid (3 mL) at room temperature. The resulting mixture was stirred at room temperature for 2 hours. The reaction mixture was concentrated and the residue was directly used in the next step. The amount of crude product 56-A was 350 mg.

Example 57

(R)-6-[(S)-2-((R)-2-Carboxy-1-methyl-ethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-Carboxylic Acid Ethyl Ester

(281) ##STR00120##

Preparation of Example 57

(282) ##STR00121##

(283) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and (3R)-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid TFA salt (Compound 57-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 57 was obtained as a light yellow solid (48 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.02 (d, 1H), 7.91 (d, 1H), 7.33-7.41 (m, 2H), 7.21-7.29 (m, 1H), 6.27 (s, 1H), 4.72 (d, 1H), 4.54 (d, 1H), 4.27-4.38 (m, 1H), 4.03-4.19 (m, 4H), 3.60-3.74 (m, 3H), 3.38-3.48 (m, 1H), 3.04-3.25 (m, 3H), 2.56-2.66 (m, 1H), 2.47-2.56 (m, 1H), 1.27 (d, 3H), 1.13 (m, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of (3R)-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic Acid TFA Salt (Compound 57-A)

(284) Compound 57-A was prepared in analogy to compound Q in Example 19 by using methyl (3R)-3-aminobutanoate hydrochloride salt (compound 57-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of methyl (3R)-3-aminobutanoate Hydrochloride Salt (Compound 57-B)

(285) Compound 57-B was prepared in analogy to compound W in Example 20 by using (R)-3-aminobutanoic acid instead of DL-3-aminoisobutyric acid.

Example 58

(R)-6-[(S)-2-((S)-2-Carboxy-1-methyl-ethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-Carboxylic Acid Ethyl Ester

(286) ##STR00122##

Preparation of Example 58

(287) ##STR00123##

(288) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and (3S)-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid TFA salt (Compound 58-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 58 was obtained as a light yellow solid (50 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01 (d, 1H), 7.90 (d, 1H), 7.33-7.40 (m, 2H), 7.21-7.28 (m, 1H), 6.27 (s, 1H), 4.66 (d, 1H), 4.49 (d, 1H), 4.34-4.42 (m, 1H), 4.20-4.20 (m, 1H), 4.04-4.19 (m, 4H), 3.56-3.66 (m, 3H), 3.42-3.50 (m, 1H), 3.22 (m, 1H), 3.01-3.13 (m, 2H), 2.50-2.62 (m, 2H), 1.23 (d, 3H), 1.13 (m, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of (3S)-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic Acid TFA Salt (Compound 58-A)

(289) Compound 58-A was prepared in analogy to compound Q in Example 19 by using methyl (3S)-3-aminobutanoate hydrochloride salt (compound 58-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of methyl (3R)-3-aminobutanoate Hydrochloride Salt (compound 58-B)

(290) Compound 58-B was prepared in analogy to compound W in Example 20 by using (S)-3-aminobutanoic acid instead of DL-3-aminoisobutyric acid.

Example 59

(R)-6-[(S)-2-(1-Carboxy-cyclobutylmethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-Carboxylic Acid Methyl Ester

(291) ##STR00124##

Preparation of Example 59

(292) ##STR00125##

(293) The title compound was prepared in analogy to Example 1 by using 1-[[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]methyl]cyclobutanecarboxylic acid TFA salt (Compound 59-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 59 was obtained as a light yellow solid (15 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, 1H), 7.76 (d, 1H), 7.42 (m, 1H), 7.24 (m, 1H), 7.06 (m, 1H), 6.17 (s, 1H), 4.08 (d, 1H), 3.90 (d, 2H), 3.82-3.86 (m, 1H), 3.66-3.72 (m, 1H), 3.61 (s, 3H), 3.54 (d, 1H), 3.47 (m, 1H), 3.14-3.23 (m, 1H), 3.05 (m, 1H), 2.90 (d, 1H), 2.80 (d, 1H), 2.39-2.47 (m, 2H), 2.31-2.39 (m, 1H), 2.16 (m, 1H), 1.99-2.09 (m, 3H), 1.89-1.98 (m, 1H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of 1-[[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]methyl]cyclobutanecarboxylic Acid TFA Salt (Compound 59-A)

(294) Compound 59-A was prepared in analogy to compound Q in Example 19 by using 1-aminomethyl-cyclobutanecarboxylic acid ethyl ester (for its synthesis, please refer to: Cao, Sheldon X. et al PCT Int. Appl. (2009), WO 2009067547) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 60

6-[(S)-2-(1-Carboxy-cyclobutylmethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-((R)-2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-Carboxylic Acid Ethyl Ester

(295) ##STR00126##

Preparation of Example 60

(296) ##STR00127##

(297) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 1-[[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]methyl]cyclobutanecarboxylic acid TFA salt (Compound 59-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 59 was obtained as a light yellow solid (15 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, 1H), 7.76 (d, 1H), 7.23-7.35 (m, 2H), 7.16 (m, 1H), 6.24 (s, 1H), 4.02-4.13 (m, 3H), 3.81-3.97 (m, 3H), 3.65-3.74 (m, 1H), 3.43-3.57 (m, 2H), 3.20 (m, 1H), 3.05 (m, 1H), 2.92 (d, 1H), 2.83 (d, 1H), 2.31-2.48 (m, 3H), 2.17 (br. s., 1H), 1.92-2.12 (m, 4H), 1.09-1.17 (m, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Example 61 (Mixture of 2 Isomers)

(R)-6-[(S)-2-((1R,3S)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester and (R)-6-[(S)-2-((1S,3R)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester

(298) ##STR00128##

Preparation of Example 61

(299) ##STR00129##

(300) The title compound was prepared in analogy to Example 1 by using cis-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 61-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 61 was obtained as a light yellow solid (30 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (m, 1H), 7.76 (d, 1H), 7.42 (m, 1H), 7.24 (m, 1H), 7.06 (m, 1H), 6.17 (s, 1H), 4.24-4.35 (m, 1H), 4.10 (d, 1H), 3.82-3.94 (m, 3H), 3.61 (s, 3H), 3.53 (m, 1H), 3.03-3.23 (m, 2H), 2.77-2.93 (m, 3H), 2.36 (m, 1H), 2.04-2.23 (m, 2H), 1.67-2.00 (m, 5H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of cis-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid TFA Salt (Compound 61-A)

(301) Compound 61-A was prepared in analogy to compound Q in Example 19 by using cis-methyl 3-aminocyclopentanecarboxylate methyl ester hydrochloride salt (compound 61-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of cis-methyl 3-aminocyclopentanecarboxylate Methyl Ester Hydrochloride Salt (Compound 61-B)

(302) Compound 61-B was prepared in analogy to compound W in Example 20 by using cis-3-aminocyclopentanecarboxylic acid (Accela Chembio Co., Ltd, CAS: 49805-32-5) instead of DL-3-aminoisobutyric acid.

Example 62 (Mixture of 2 Isomers)

(R)-6-[(S)-2-((1R,3S)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester and (R)-6-[(S)-2-((1S,3R)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(303) ##STR00130##

Preparation of Example 62

(304) ##STR00131##

(305) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and cis-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 61-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 62 was obtained as a light yellow solid (50 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (m, 1H), 7.76 (d, 1H), 7.23-7.34 (m, 2H), 7.09-7.19 (m, 1H), 6.24 (s, 1H), 4.23-4.34 (m, 1H), 4.01-4.15 (m, 3H), 3.83-3.95 (m, 3H), 3.53 (m, 1H), 3.04-3.23 (m, 2H), 2.77-2.95 (m, 3H), 2.36 (m, 1H), 2.03-2.22 (m, 2H), 1.90-2.00 (m, 2H), 1.68-1.90 (m, 3H), 1.13 (m, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Example 63 (Mixture of Two Isomers)

(R)-6-[(S)-2-((1R,3R)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester and (R)-6-[(S)-2-((1S,3S)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester

(306) ##STR00132##

Preparation of Example 63

(307) ##STR00133##

(308) The title compound was prepared in analogy to Example 1 by using trans-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 63-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 63 was obtained as a light yellow solid (10 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, 1H), 7.76 (d, 1H), 7.42 (m, 1H), 7.24 (m, 1H), 7.06 (m, 1H), 6.17 (s, 1H), 4.39 (d, 1H), 4.10 (d, 1H), 3.84-3.92 (m, 3H), 3.61 (s, 3H), 3.49-3.53 (m, 1H), 3.18 (d, 1H), 3.06 (d, 1H), 2.89 (br. s., 3H), 2.38 (d, 1H), 2.07-2.19 (m, 3H), 1.86-1.97 (m, 3H), 1.70 (br. s., 1H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

Preparation of trans-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic Acid TFA Salt (Compound 63-A)

(309) Compound 63-A was prepared in analogy to compound Q in Example 19 by using trans-methyl 3-aminocyclopentanecarboxylate methyl ester hydrochloride salt (compound 63-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of trans-methyl 3-aminocyclopentanecarboxylate Methyl Ester Hydrochloride Salt (Compound 63-B)

(310) Compound 63-B was prepared in analogy to compound W in Example 20 by using trans-3-aminocyclopentanecarboxylic acid (for its synthesis, please refer to: Allan, Robin D.; Fong, Joyce Australian Journal of Chemistry (1986), 39(6), 855-64.) instead of DL-3-aminoisobutyric acid.

Example 64 (Mixture of Two Isomers)

(R)-6-[(S)-2-((1R,3R)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester and (R)-6-[(S)-2-((1S,3S)-3-Carboxy-cyclopentyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(311) ##STR00134##

Preparation of Example 64

(312) ##STR00135##

(313) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and trans-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclopentanecarboxylic acid TFA salt (Compound 63-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 64 was obtained as a light yellow solid (10 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (m, 1H), 7.76 (d, 1H), 7.24-7.34 (m, 2H), 7.12-7.19 (m, 1H), 6.24 (s, 1H), 4.34-4.42 (m, 1H), 4.02-4.14 (m, 3H), 3.83-3.94 (m, 3H), 3.51 (m, 1H), 3.14-3.22 (m, 1H), 3.04-3.10 (m, 1H), 2.82-2.94 (m, 3H), 2.36 (m, 1H), 2.05-2.20 (m, 3H), 1.81-1.98 (m, 3H), 1.65-1.74 (m, 1H), 1.13 (m, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Example 65

(R)-6-[2-(4-Carboxy-benzyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Methyl Ester

(314) ##STR00136##

Preparation of Example 65

(315) ##STR00137##

(316) The title compound was prepared in analogy to Example 1 by using 4-[(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)methyl]benzoic acid TFA salt (Compound 65-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 65 was obtained as a light yellow solid (90 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.06 (m, 3H), 7.91 (m, 1H), 7.53 (m, 1H), 7.40-7.45 (m, 2H), 7.30 (m, 1H), 7.08-7.15 (m, 1H), 6.20 (d, 1H), 4.75 (m, 1H), 4.48-4.62 (m, 3H), 4.12-4.25 (m, 2H), 3.68-3.78 (m, 2H), 3.65 (d, 3H), 3.44-3.58 (m, 2H), 3.16-3.28 (m, 2H), 3.09-3.15 (m, 1H). MS: calc'd 639 (MH.sup.+), measured 639 (MH.sup.+).

Preparation of 4-[(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)methyl]benzoic Acid TFA Salt (Compound 65-A)

(317) Compound 65-A was prepared in analogy to compound Q in Example 19 by using 4-aminomethyl-benzoic acid methyl ester hydrochloride instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 66

(R)-6-[2-(4-Carboxy-benzyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(318) ##STR00138##

Preparation of Example 66

(319) ##STR00139##

(320) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 4-[(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)methyl]benzoic acid TFA salt (Compound 65-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 66 was obtained as a light yellow solid (82 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.99-8.07 (m, 3H), 7.90 (m, 1H), 7.40-7.46 (m, 2H), 7.32-7.39 (m, 2H), 7.21-7.29 (m, 1H), 6.26 (d, 1H), 4.75 (m, 1H), 4.47-4.61 (m, 3H), 4.13-4.25 (m, 2H), 4.06-4.13 (m, 2H), 3.68-3.78 (m, 2H), 3.44-3.58 (m, 2H), 3.18-3.31 (m, 1H), 3.07-3.17 (m, 2H), 1.13 (m, 3H). MS: calc'd 653 (MH.sup.+), measured 653 (MH.sup.+).

Example 67

2-[2-[7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]ethoxy]acetic Acid

(321) ##STR00140##

Preparation of Example 67

(322) ##STR00141##

(323) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 2-[2-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)ethoxy]acetic acid (Compound 67-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 67 was obtained as a light yellow solid (2.5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-8.01 (d, J=4.0 Hz, 1H), 7.90-7.89 (d, J=4.0 Hz, 1H), 7.35-7.38 (m, 2H), 7.24 (m, 1H), 6.26 (s, 1H), 4.66 (m, 1H), 4.51 (m, 1H), 4.05-4.40 (m, 5H), 3.70-3.83 (m, 3H), 3.52-3.70 (m, 3H), 3.40 (m, 3H), 3.32 (m, 2H), 1.13 (m, 3H). MS: calc'd 621 (MH.sup.+), measured 621 (MH.sup.+).

Preparation of 2-[2-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)ethoxy]acetic Acid (Compound 67-A)

(324) ##STR00142## ##STR00143##
Step 1:

(325) The mixture of methyl ()-4-Boc-piperazine-2-carboxylate (CAS: 129799-08-2, 2.44 g, 0.01 mol), TEA (1.52 g, 0.015 mol) in DCM (10 mL) was added to a solution of (4-nitrophenyl)carbonochloridate (2.42 g, 0.012 mol) in DCM (10 mL). After 1 hour at rt, the organic layer was washed with water and saturated sodium bicarbonate solution, dried over sodium sulfate. After removal of solvent, the crude product 67-B was obtained and used in the next step without purification. MS: calc'd 410 (MH.sup.+), measured 410 (MH.sup.+).

(326) Step 2:

(327) The mixture of compound 67-B (1.0 g, 2.44 mmol), 2-aminoethanol (1.0 g, 16.4 mmol), TEA (0.49 g, 4.88 mmol), nitromethane (2 mL) was heated at 130 C. with microwave for 3 hours. After removal of solvent, the residue was treated with water, extracted with ethyl acetate. Removal of solvent gave the crude product 67-C. MS: calc'd 300 (MH.sup.+), measured 300 (MH.sup.+).

(328) Step 3:

(329) A mixture of compound 67-C (730 mg, 2.44 mmol) and BH.sub.3.THF (1M, 4.88 mL). After stirring for 24 hours at rt, the mixture was treated with saturated sodium bicarbonate aqueous solution, extracted with ethyl acetate. After removal of solvent, the product 67-D was obtained. MS: calc'd 286 (MH.sup.+), measured 286 (MH.sup.+).

(330) Step 4:

(331) A mixture of compound 67-D (0.5 g, 1.75 mmol) in THF (15 mL) was added t-BuOK (0.294 g, 2.63 mmol). After stirring for 30 minutes, t-butyl bromoacetate (0.68 g, 3.5 mmol) was added into it. Then the mixture was stirred for another 2 hours at rt, quenched with water, extracted with ethyl acetate. After removal of solvent, the crude product 67-E was obtained and used in the next step. MS: calc'd 400 (MH.sup.+), measured 400 (MH.sup.+).

(332) Step 5:

(333) A mixture of compound 67-E (0.70 g, 1.75 mmol) in DCM (5 mL) was treated with TFA (2 mL). After stirring for 1 hour at rt, the solvent and residue TFA was removed under reduced pressure to afford the product 67-A. MS: calc'd 244 (MH.sup.+), measured 244 (MH.sup.+).

Example 68

2-[3-[7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]propoxy]acetic Acid

(334) ##STR00144##

Preparation of Example 68

(335) ##STR00145##

(336) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 2-[3-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)propoxy]acetic acid (Compound 68-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 68 was obtained as a light yellow solid (6.5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.99-7.98 (d, J=4.0 Hz, 1H), 7.81-7.82 (d, J=4.0 Hz, 1H), 7.32 (m, 2H), 7.21 (m, 1H), 6.25 (s, 1H), 3.90-4.35 (m, 6H), 3.56 (m, 3H), 3.10-3.40 (m, 4H), 2.60 (m, 2H), 1.85 (m, 2H), 1.15 (m, 3H). MS: calc'd 635 (MH.sup.+), measured 635 (MH.sup.+).

Preparation of 2-[3-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)propoxy]acetic Acid (Compound 68-A)

(337) Compound 68-A was prepared in analogy to Compound 67-A by using 3-aminopropan-1-ol instead of 2-aminoethanol.

Example 69

Methyl (4R)-4-(2-chloro-4-fluoro-phenyl)-6-[[2-(5-hydroxy-4,4-dimethyl-pentyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(338) ##STR00146##

Preparation of Example 69

(339) ##STR00147##

(340) The title compound was prepared in analogy to Example 1 by using 2-(5-hydroxy-4,4-dimethyl-pentyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 69-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 69 was obtained as a light yellow solid (55 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97-7.98 (d, J=4.0 Hz, 1H), 7.80-7.81 (d, J=4.0 Hz, 1H), 7.45-7.48 (m, 1H), 7.27 (m, 1H), 7.08 (m, 1H), 6.18 (s, 1H), 3.80-4.40 (m, 4H), 3.65 (s, 3H), 3.55 (m, 1H), 3.10-3.30 (m, 6H), 2.50 (m, 2H), 1.50 (m, 2H), 1.25 (m, 2H), 0.89 (s, 6H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

Preparation of 2-(5-hydroxy-4,4-dimethyl-pentyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 69-A)

(341) ##STR00148##

(342) tert-Butyl 2-(5-hydroxy-4,4-dimethyl-pentyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 69-B) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. The reaction mixture was stirred at rt for 1 hour and then the solvent was removed under vacuum to give the crude product 69-A, which was used directly in the next step.

Preparation of tert-butyl 2-(5-hydroxy-4,4-dimethyl-pentyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 69-B)

(343) Compound 69-B was prepared in analogy to Compound 67-D by using 5-amino-2,2-dimethyl-pentan-1-ol instead of 2-aminoethanol.

Example 70

Ethyl (4R)-4-(2-chloro-3-fluoro-phenyl)-6-[[2-(2-hydroxyethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(344) ##STR00149##

Preparation of Example 70

(345) ##STR00150##

(346) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 2-(2-hydroxyethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 70-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 70 was obtained as a light yellow solid (45 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01-8.02 (d, J=4.0 Hz, 1H), 7.91-7.92 (d, J=4.0 Hz, 1H), 7.38 (m, 2H), 7.27 (m, 1H), 6.27 (s, 1H), 4.55-4.78 (m, 2H), 4.10-4.25 (m, 4H), 3.72 (m, 5H), 3.45 (m, 2H), 3.10-3.35 (m, 7H). MS: calc'd 563 (MH.sup.+), measured 563 (MH.sup.+).

Preparation of 2-(2-hydroxyethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 70-A)

(347) ##STR00151##

(348) tert-Butyl 2-(2-hydroxyethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 67-D) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. The reaction mixture was stirred at rt for 1 hour and then the solvent was removed in vacuum to give the crude product 70-A, which was used directly in the next step.

Example 71

Ethyl (4R)-4-(2-chloro-3-fluoro-phenyl)-6-[[2-(2-hydroxyethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(349) ##STR00152##

Preparation of Example 71

(350) ##STR00153##

(351) The title compound was prepared in analogy to Example 1 by using 2,2-dimethyl-5-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)pentanoic acid (Compound 71-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 71 was obtained as a light yellow solid (45 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01-8.02 (d, J=4.0 Hz, 1H), 7.91-7.92 (d, J=4.0 Hz, 1H), 7.53 (m, 1H), 7.40 (m, 1H), 7.10 (m, 1H), 6.20 (s, 1H), 4.52-4.75 (m, 2H), 4.10-4.25 (m, 2H), 3.65 (m, 5H), 3.45 (m, 1H), 3.10-3.35 (m, 4H), 1.55 (m, 4H), 1.20 (s, 6H). MS: calc'd 563 (MH.sup.+), measured 563 (MH.sup.+).

Preparation of 2,2-dimethyl-5-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)pentanoic Acid (Compound 71-A)

(352) ##STR00154##
Step 1:

(353) A mixture of tert-butyl 2-(5-hydroxy-4,4-dimethyl-pentyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (compound 69-B) (0.4 g, 1.1 mmol) and pyridinium dichromate (1.7 g, 4.4 mmol) in DMF (10 mL) was stirred for 12 hours, then treated with water, adjusted pH<7 with acetic acid, extracted with ethyl acetate. After removal of solvent, the crude product 71-B was obtained. MS: calc'd 370 (MH.sup.+), measured 370 (MH.sup.+).

(354) Step 2:

(355) A mixture of compound 71-B (0.41 g, 1.1 mmol), TFA (2 mL) in DCM (2 mL) was stirred for 1.2 hours at rt. After removal of solvent and TFA, the crude product 71-A was obtained and used in the next step. MS: calc'd 270 (MH.sup.+), measured 270 (MH.sup.+).

Example 72

4-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic Acid

(356) ##STR00155##

Preparation of Example 72

(357) ##STR00156##

(358) The title compound was prepared in analogy to Example 1 by using cis-4-[(2R)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic acid TFA salt (Compound 72-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 72 was obtained as a light yellow solid (30 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.42 (dd, J=8.8, 6.3 Hz, 1H), 7.24 (dd, J=8.8, 2.5 Hz, 1H), 7.06 (td, J=8.5, 2.6 Hz, 1H), 6.17 (s, 1H), 4.10 (d, J=16.8 Hz, 1H), 3.79-3.97 (m, 3H), 3.55-3.70 (m, 4H), 3.48 (t, J=8.9 Hz, 1H), 3.11-3.26 (m, 1H), 3.03 (dd, J=9.3, 4.0 Hz, 1H), 2.71-2.96 (m, 3H), 2.04-2.42 (m, 5H), 1.77 (br. s., 2H), 1.46-1.62 ppm (m, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Preparation of cis-4-[(2R)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic Acid TFA Salt (Compound 72-A)

(359) Compound 72-A was prepared in analogy to compound Q in Example 19 by using methyl cis-4-aminocyclohexanecarboxylate hydrochloride (CAS: 61367-16-6, TCI) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 73

4-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic Acid

(360) ##STR00157##

Preparation of Example 73

(361) ##STR00158##

(362) The title compound was prepared in analogy to Example 1 by using trans-4-[(2R)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic acid TFA salt (Compound 73-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 73 was obtained as a light yellow solid (46 mg). H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d J=3.0 Hz, 1H), 7.77 (d J=3.0 Hz, 1H), 7.42 (dd J=8.8, 6.3 z, 1), 7.24 (dd, J=8.5, 2.8 Hz, 1H), 7.06 (td, J=8.5, 2.5 Hz, 1H), 6.17 (s, 1H), 4.10 (d, J=17.1 Hz, 1H), 3.78-3.97 (m, 3H), 3.61 (s, 3H), 3.41-3.52 (m, 1H), 3.19 (d, J=11.0 Hz, 1H), 3.03 (dd, J=9.5, 4.0 Hz, 1H), 2.75-2.94 (m, 2H), 2.36 (dd, J=11.7, 7.9 Hz, 2H), 1.93-2.24 (m, 5H), 1.77 (br. s., 2H), 1.53 ppm (d, J=10.0 Hz, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Preparation of trans-4-[(2R)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]cyclohexanecarboxylic Acid TFA Salt (Compound 73-A)

(363) Compound 73-A was prepared in analogy to compound Q in Example 19 by using methyl-trans-4-amino-cyclohexanecarboxylic acid methyl ester (CAS: 61367-07-5, TCI) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 74

3-[(8aS)-7-[[(4R)-4-(2-chlorophenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-Propanoic Acid

(364) ##STR00159##

Preparation of Example 74

(365) ##STR00160##

(366) The title compound was prepared in analogy to Example 1 by using methyl (4R)-6-(bromomethyl)-4-(2-chlorophenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-2) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 74 was obtained as a light yellow solid (6 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.41 (td, J=7.4, 1.8 Hz, 2H), 7.19-7.33 (m, 2H), 6.21 (s, 1H), 4.08 (d, J=17.1 Hz, 1H), 3.79-3.96 (m, 3H), 3.60 (s, 2H), 3.51 (t, J=9.2 Hz, 1H), 3.37-3.42 (m, 1H), 3.07-3.28 (m, 3H), 2.74-2.94 (m, 2H), 2.12-2.41 (m, 4H), 1.39 (d, J=6.3 Hz, 2H), 1.18 ppm (d, J=4.0 Hz, 3H). MS: calc'd 587 (MH.sup.+), measured 587 (MH.sup.+).

Preparation of methyl (4R)-6-(bromomethyl)-4-(2-chlorophenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-Carboxylate (Compound C-2)

(367) Compound C-2 was prepared in analogy to compound C by using 2-chlorobenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde.

Example 75

2-[[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]butanoic Acid

(368) ##STR00161##

Preparation of Example 75

(369) ##STR00162##

(370) The title compound was prepared in analogy to Example 1 by using 2-[[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]methyl]butanoic acid TFA salt (Compound 75-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 75 was obtained as a light yellow solid (50 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96-7.97 (m, 1H), 7.92 (d, J=3.3 Hz, 1H), 7.54 (dd, J=8.8, 6.0 Hz, 1H), 7.30 (dd, J=8.7, 2.6 Hz, 1H), 7.12 (td, J=8.3, 2.6 Hz, 1H), 6.20 (s, 1H), 4.64-4.81 (m, 2H), 4.46-4.60 (m, 1H), 4.01-4.26 (m, 2H), 3.60-3.78 (m, 5H), 3.35-3.57 (m, 3H), 3.03-3.29 (m, 3H), 2.62 (dtd, J=14.1, 8.4, 5.5 Hz, 1H), 1.50-1.74 (m, 2H), 0.99 ppm (t, J=7.4 Hz, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of 2-[[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]methyl]butanoic Acid TFA Salt (Compound 75-A)

(371) Compound 75-A was prepared in analogy to compound Q by using methyl 2-(aminomethyl)butanoate (for its synthesis, please refer to: Kaptein, Bemardus; et al PCT Int. Appl. 2005, WO 2005085462) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 76

3-[(8aS)-7-[[(4S)-5-ethoxycarbonyl-4-(3-fluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(372) ##STR00163##

Preparation of Example 76

(373) ##STR00164##

(374) The title compound was prepared in analogy to Example 1 by using ethyl (4S)-6-(bromomethyl)-4-(3-fluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-3) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 76 was obtained as a light yellow solid (132 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) ppm 7.95 (d, J=3.3 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 7.08-7.23 (m, 2H), 6.95 (t, J=8.8 Hz, 1H), 5.99 (s, 1H), 4.02-4.17 (m, 3H), 3.79-4.00 (m, 3H), 3.36-3.57 (m, 2H), 3.26-3.33 (m, 1H), 3.17-3.25 (m, 1H), 3.11 dd, J=9.3, 4.0 Hz, 1H), 2.78-2.99 (m, 2H), 2.53 (d, J=2.0 Hz, 3H), 2.39 (dd, J=11.2, 8.2 Hz, 1H), 2.14-2.26 (m, 1H), 1.21 (d, J=2.8 Hz, 6H), 1.15 ppm (t, J=7.2 Hz, 3H). MS: calc'd 599 (MH.sup.+), measured 599 (MH.sup.+).

Preparation of Ethyl (4S)-6-(bromomethyl)-4-(3-fluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-3)

(375) Compound C-3 was prepared in analogy to compound C by using 2-methyl-3-fluorobenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde and ethyl acetoacetate instead of methyl acetoacetate.

Example 77

3-[(8aS)-7-[[4-(4-chlorophenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(376) ##STR00165##

Preparation of Example 77

(377) ##STR00166##

(378) The title compound was prepared in analogy to Example 1 by using methyl 6-(bromomethyl)-4-(4-chlorophenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-4) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 77 was obtained as a light yellow solid (60 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.99 (dd, J=3.1, 1.4 Hz, 1H), 7.80 (d, J=3.3 Hz, 1H), 7.26-7.42 (m, 4H), 5.64-5.82 (m, 1H), 4.06 (dd, J=16.8, 4.3 Hz, 1H), 3.72-3.90 (m, 3H), 3.69 (s, 3H), 3.37-3.61 (m, 2H), 3.00-3.24 (m, 2H), 2.55-2.97 (m, 3H), 2.11-2.39 (m, 2H), 1.19 ppm (dd, J=4.4, 2.9 Hz, 6H). MS: calc'd 587 (MH.sup.+), measured 587 (MH.sup.+).

Preparation of Methyl 6-(bromomethyl)-4-(4-chlorophenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-4)

(379) Compound C-4 was prepared in analogy to compound C by using 4-chlorobenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde without chiral SFC separation of dihydropyrimidine intermediate in Scheme 1-1.

Example 78

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2-methoxy-propanoic Acid

(380) ##STR00167##

Preparation of Example 78

(381) ##STR00168##

(382) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 2-methoxy-3-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)propanoic acid TFA salt (Compound 78-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 78 was obtained as a light yellow solid (25 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01 (d, J=3.3 Hz, 1H), 7.92 (d, J=3.0 Hz, 1H), 7.33-7.43 (m, 2H), 7.19-7.30 (m, 1H), 6.27 (s, 1H), 4.76 (d, J=16.1 Hz, 1H), 4.58 (d, J=16.1 Hz, 1H), 3.96-4.21 (m, 6H), 3.41-3.83 (m, 10H), 3.10-3.31 (m, 5H), 1.13 ppm (t, J=7.2 Hz, 3H). MS: calc'd 621 (MH.sup.+), measured 621 (MH.sup.+).

Preparation of 2-methoxy-3-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)propanoic Acid TFA Salt (Compound 78-A)

(383) Compound 78-A was prepared in analogy to compound Q in Example 19 by using methyl 2-(aminomethyl)butanoate (for its synthesis, please refer to: Liang, Congxin; Feng, Yangbo U.S. Pat. Appl. Publ. 2007, US 20070072934) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 79

2-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]spiro[3.3]heptane-6-carboxylic Acid

(384) ##STR00169##

Preparation of Example 79

(385) ##STR00170##

(386) The title compound was prepared in analogy to Example 1 by using 2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]spiro[3.3]heptane-6-carboxylic acid TFA salt (Compound 79-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 79 was obtained as a light yellow solid (7 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.00-7.92 (m, 1H), 7.77 (d, J=3.3 Hz, 1H), 7.42 (dd, J=6.3, 8.5 Hz, 1H), 7.24 (dd, J=2.5, 8.8 Hz, 1H), 7.06 (dt, J=2.5, 8.4 Hz, 1H), 6.18 (s, 1H), 4.33-4.20 (m, 1H), 4.10 (dd, J=4.4, 16.9 Hz, 1H), 3.96-3.73 (m, 4H), 3.66-3.58 (m, 3H), 3.57-3.46 (m, 1H), 3.21-2.97 (m, 3H), 2.95-2.80 (m, 2H), 2.73 (d, J=10.3 Hz, 1H), 2.44-2.05 (m, 8H). MS: calc'd 626 (MH.sup.+), measured 626 (MH.sup.+).

Preparation of 2-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]spiro[3.3]heptane-6-carboxylic Acid TFA Salt (Compound 79-A)

(387) Compound 79-A was prepared in analogy to compound Q in Example 19 by using methyl 2-aminospiro[3.3]heptane-6-carboxylate hydrochloride salt (PharmaBlock (Nanjing) R&D Co. Ltd, PBLG1036) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 80

5-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]pentanoic Acid

(388) ##STR00171##

Preparation of Example 80

(389) ##STR00172##

(390) The title compound was prepared in analogy to Example 1 by using 5-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]pentanoic acid TFA salt (Compound 80-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 80 was obtained as a light yellow solid (18 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.02 (d, J=3.0 Hz, 1H), 7.92 (d, J=3.0 Hz, 1H), 7.54 (ddd, J=1.5, 6.0, 8.8 Hz, 1H), 7.30 (dd, J=2.5, 8.5 Hz, 1H), 7.22-7.09 (m, 1H), 6.20 (s, 1H), 4.76 (d, J=16.1 Hz, 1H), 4.66-4.39 (m, 1H), 4.20 (td, J=3.4, 11.7 Hz, 1H), 4.10 (td, J=4.1, 14.8 Hz, 1H), 3.83-3.56 (m, 6H), 3.54-3.38 (m, 1H), 3.30-3.10 (m, 5H), 2.43-2.28 (m, 2H), 1.62 (dd, J=3.1, 5.9 Hz, 4H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of 5-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]pentanoic Acid TFA Salt (Compound 80-A)

(391) Compound 80-A was prepared in analogy to compound Q in Example 19 by using methyl 5-aminopentanoate hydrochloride salt instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Example 81

3-[[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]methyl]cyclobutanecarboxylic Acid

(392) ##STR00173##

Preparation of Example 81

(393) ##STR00174##

(394) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and 3-[(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)methyl]cyclobutanecarboxylic acid TFA salt (Compound 81-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 81 was obtained as a light yellow solid (7 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.99 (d, J=3.0 Hz, 1H), 7.84 (d, J=2.3 Hz, 1H), 7.43-7.28 (m, 2H), 7.27-7.13 (m, 1H), 6.25 (s, 1H), 4.52-4.33 (m, 1H), 4.32-4.18 (m, 1H), 4.07 (q, J=7.0 Hz, 3H), 3.68-3.47 (m, 2H), 3.32-2.98 (m, 7H), 2.65 (d, J=7.5 Hz, 3H), 2.44-2.27 (m, 2H), 2.10-1.98 (m, 2H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 631 (MH.sup.+), measured 631 (MH.sup.+).

Preparation of 3-[(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)methyl]cyclobutanecarboxylic Acid TFA Salt (Compound 81-A)

(395) Compound 81-A was prepared in analogy to compound Q in Example 19 by using methyl 3-(aminomethyl)cyclobutanecarboxylate hydrochloride salt (compound 81-B) instead of ethyl 3-amino-2,2-dimethyl-propanoate hydrochloride salt.

Preparation of Methyl 3-(aminomethyl)cyclobutanecarboxylate Hydrochloride Salt (Compound 81-B)

(396) ##STR00175##

(397) Compound 81-B was prepared in analogy to compound W in Example 20 by using 3-(aminomethyl)cyclobutanecarboxylic acid (PharmaBlock (Nanjing) R&D Co. Ltd, CAS: 1310729-95-3) instead of DL-3-aminoisobutyric acid.

Example 82A and 82B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-cyclopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(398) ##STR00176##

Preparation of Example 82A and 82B

(399) ##STR00177##

(400) To a stirred solution of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C, 176 mg, 0.5 mmol) in dry dimethylformamide (3.0 mL) was added diisopropylethylamine (1.0 mL), potassium iodide (125 mg, 0.75 mmol) and compound C-1 (250 mg, 0.55 mmol). The reaction mixture was flushed with nitrogen and heated to 55 C. for 2 hours. The reaction mixture was quenched by adding ice-water, extracted with ethyl acetate (30 mL) three times. The organic phase was separated, dried over Na.sub.2SO.sub.4, filtrated and concentrated. The residue was purified by HPLC to give a mixture of two isomers which were further resolved by SFC to give two single isomers: 82A-1 (faster eluting, 77.3 mg, yield: 25%) and 82B-1 (slower eluting, 93 mg, yield: 30%) with 30% isopropanol (0.05% DEA)/CO.sub.2 on ChiralPak AD-3 column. MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

(401) The solution of compound 82A-1 (77.3 mg, 0.125 mmol) in tetrahydrofuran (0.62 mL) was added lithium hydroxide monohydrate (52.7 mg, 1.25 mmol) in water (0.62 mL). After the reaction mixture was stirred at 35 C. for 2 hours, it was neutralized with 1N hydrochloride solution to pH 3.0. The mixture was extracted with ethyl acetate (30 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and then concentrated. The residue was purified by Prep-HPLC to give Example 82A (56.2 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.34-7.27 (m, 2H), 7.16 (s, 1H), 6.25 (s, 1H), 4.31 (d, J=17.3 Hz, 1H), 4.10-4.00 (m, 2H), 3.91 (d, J=16.8 Hz, 1H), 3.86-3.75 (m, 2H), 3.62-3.54 (m, 1H), 3.46 (dd, J=5.4, 9.7 Hz, 1H), 3.32-3.28 (m, 1H), 3.21-3.08 (m, 1H), 2.90 (br. s., 1H), 2.55 (br. s., 1H), 2.46 (t, J=4.9 Hz, 1H), 1.13 (t, J=7.2 Hz, 3H), 0.77-0.66 (m, 4H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

(402) Example 82B (68.3 mg) was prepared in analogy to Example 82A. .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.29 (s, 2H), 7.21-7.13 (m, 1H), 6.22 (s, 1H), 4.24 (d, J=17.1 Hz, 1H), 4.08-3.95 (m, 3H), 3.89-3.77 (m, 2H), 3.60-3.52 (m, 1H), 3.43 (dd, J=5.3, 9.5 Hz, 1H), 3.32-3.28 (m, 1H), 3.22-3.12 (m, 1H), 3.06 (br. s., 1H), 2.66 (br. s., 1H), 2.50-2.42 (m, 1H), 1.12 (t, J=7.0 Hz, 3H), 0.73 (s, 4H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

Preparation of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate Trifluoroacetic Acid Salt (Compound 82-C)

(403) ##STR00178## ##STR00179##
Step 1:

(404) The mixture of compound AV (6.5 g, 16.7 mmol) and borane-THF complex solution 1.0 M in THF (40 mL, 40 mmol) was stirred at 32 C. overnight. The reaction mixture was concentrated. The residue was dissolved in ethyl acetate (60 mL) and added 2.0 mL of hydrogen chloride solution (4.0 M in dioxane). The mixture was extracted with ethyl acetate (50 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and concentrated. The residue was purified by column to give compound 82-C-1 (4.0 g).

(405) Step 2:

(406) To a solution of oxalyl chloride (2.0 mL) in dry dichloromethane (60 mL) under nitrogen at 78 C. was added dropwise of dimethyl sulfoxide (3.3 mL, 47 mmol) in dry dichloromethane (2.0 mL). After addition, the reaction mixture was stirred for 1 hour at 78 C. Then to the reaction mixture was added compound 82-C-1 (4.0 g, 10.7 mmol) in dry dichloromethane (6.0 mL) dropwise and stirred for another hour at 78 C. The reaction was quenched by adding triethylamine (12 mL, 86 mmol) dropwise over 30 minutes. The resulting mixture was warmed up to room temperature, and the organic phase was washed by ice-water, separated and dried over Na.sub.2SO.sub.4, then filtrated and concentrated to give crude compound 82-C-2 (3.72 g, crude), which was used directly.

(407) Step 3:

(408) To a solution of compound 82-C-2 (930 mg, 2.5 mmol) in dichloromethane (10 mL) was added cyclopropylamine (260 L, 3.75 mmol), catalytic amount of acetic acid (2 drops) and sodium triacetoxyborohydride (1.06 g, 5 mmol). The reaction mixture was stirred at room temperature overnight. The reaction was quenched by adding ice-water, extracted with dichloromethane (30 mL) two times and the organic phase was washed with sodium bicarbonate solution and dried over Na.sub.2SO.sub.4, then filtrated and concentrated to give crude compound 82-C-3 (1.03 g, crude).

(409) Step 4:

(410) The mixture of compound 82-C-3 (1.03 g, 2.5 mmol) in dichloromethane (15 mL) was added diisopropylethylamine (1.0 mL) and 4-nitrophenyl chloroformate (1.0 g, 5.0 mmol). The reaction mixture was stirred at 40 C. for 2 hours. The reaction was quenched by adding ice-water and extracted with dichloromethane (30 mL) two times. The organic phase was washed with sodium bicarbonate solution and dried over Na.sub.2SO.sub.4, then filtrated and concentrated. The residue was purified by column to give compound 82-C-4 (1.4 g).

(411) Step 5:

(412) The mixture of compound 82-C-4 (1.4 g, 2.42 mmol) and TFA/DCM=2/1 (6 mL) was stirred at room temperature for 30 minutes. The reaction mixture was concentrated and added toluene for co-evaporation to remove trifluoroacetic acid. The residue was dissolved in dichloromethane (15 mL), and then diisopropylethylamine (2 mL) was added. The reaction mixture was heated to 40 C. overnight. To the reaction mixture was added di-tert-butyl dicarbonate (1.1 g, 5.0 mmol) and stirred for another 5 hours. The reaction mixture was quenched by adding ice-water, extracted with ethyl acetate (30 mL) two times. The organic phase was separated, dried over Na.sub.2SO.sub.4, filtrated and concentrated. The residue was purified by column to give compound 82-C-5 (680 mg).

(413) Step 6:

(414) The mixture of compound 82-C-5 (170 mg, 0.5 mmol) and TFA/DCM=2/1 (3 mL) was stirred at room temperature for 30 minutes. The reaction mixture was concentrated and added toluene for co-evaporation to remove trifluoroacetic acid to give crude compound 82-C which was used directly.

Example 83A and 83B (Separated Two Single Isomers)

(8R,8aS)-2-cyclopropyl-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-2-cyclopropyl-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(415) ##STR00180##

Preparation of Example 83A and 83B

(416) ##STR00181##

(417) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-5) instead of ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1).

(418) Example 83A was obtained as a light yellow solid (99 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.14-7.07 (m, 1H), 7.06-6.98 (m, 1H), 5.93 (s, 1H), 4.29 (d, J=17.1 Hz, 1H), 3.88 (d, J=17.3 Hz, 1H), 3.79 (br. s., 2H), 3.62 (s, 3H), 3.55 (s, 1H), 3.46 (d, J=5.5 Hz, 1H), 3.26 (d, J=9.8 Hz, 1H), 3.20-3.09 (m, 1H), 2.85 (d, J=10.8 Hz, 1H), 2.56 (d, J=2.3 Hz, 3H), 2.54-2.42 (m, 2H), 0.78-0.68 (m, 4H). MS: calc'd 587 (MH.sup.+), measured 587 (MH.sup.+).

(419) Example 83B was obtained as a light yellow solid (72.9 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.94 (d, J=3.3 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 7.09 (d, J=4.8 Hz, 2H), 5.90 (s, 1H), 4.21 (d, J=17.1 Hz, 1H), 3.97 (d, J=17.1 Hz, 1H), 3.88-3.76 (m, 2H), 3.61 (s, 3H), 3.58-3.50 (m, 1H), 3.46-3.40 (m, 1H), 3.28 (s, 1H), 3.21-3.12 (m, 1H), 3.08-2.99 (m, 1H), 2.63 (d, J=3.3 Hz, 1H), 2.56 (d, J=2.3 Hz, 3H), 2.50-2.42 (m, 1H), 0.79-0.66 (m, 4H). MS: calc'd 587 (MH.sup.+), measured 587 (MH.sup.+).

(420) Example 83A was synthesized from compound 83A-1 (faster eluting) and Example 83B was synthesized from compound 83B-1 (slower eluting) on ChiralCel OJ-H column eluting with 25% methanol (0.05% DEA)/CO.sub.2.

Preparation of Methyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-5)

(421) Compound C-5 was prepared in analogy to compound C by using 3,4-difluoro-2-methylbenzaldehyde instead of 2-chloro-4-fluorobenzaldehyde.

Example 84A and 84B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(422) ##STR00182##

Preparation of Example 84A and 84B

(423) ##STR00183##

(424) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl 2-isopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 84-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(425) Example 84A was obtained as a light yellow solid (20.3 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.11 (d, J=3.0 Hz, 1H), 8.01 (d, J=3.0 Hz, 1H), 7.39 (d, J=2.0 Hz, 2H), 7.28-7.21 (m, 1H), 6.32 (s, 1H), 4.38 (d, J=17.3 Hz, 1H), 4.08 (d, J=6.5 Hz, 4H), 3.93-3.82 (m, 2H), 3.62-3.47 (m, 3H), 3.24-3.14 (m, 1H), 3.10-3.04 (m, 1H), 2.69 (d, J=3.5 Hz, 1H), 1.25-1.11 (m, 9H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

(426) Example 84B was obtained as a light yellow solid (32.5 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.10 (s, 1H), 8.02 (s, 1H), 7.38 (d, J=4.3 Hz, 2H), 7.29-7.23 (m, 1H), 6.30 (s, 1H), 4.36 (d, J=16.6 Hz, 1H), 4.14-4.03 (m, 4H), 3.87 (d, J=3.3 Hz, 2H), 3.56 (d, J=9.8 Hz, 2H), 3.50-3.44 (m, 1H), 3.20 (s, 2H), 2.77 (d, J=3.5 Hz, 1H), 1.24-1.10 (m, 9H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

(427) Example 84A was synthesized from compound 84A-1 (faster eluting) and Example 84B was synthesized from compound 84B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Methyl 2-isopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound 84-C)

(428) Compound 84-C was prepared in analogy to compound 82-C by using isopropylamine instead of cyclopropylamine.

Example 85A and 85B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(429) ##STR00184##

Preparation of Example 85A and 85B

(430) ##STR00185##

(431) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-5) instead of ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and methyl 2-isopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 84-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(432) Example 85A was obtained as a light yellow solid (3.6 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.10 (d, J=3.3 Hz, 1H), 7.99 (d, J=3.0 Hz, 1H), 7.26-7.19 (m, 1H), 7.14-7.04 (m, 1H), 6.01 (s, 1H), 4.34 (d, J=17.1 Hz, 1H), 4.11 (s, 1H), 3.98 (d, J=17.3 Hz, 1H), 3.85 (d, J=4.8 Hz, 2H), 3.65 (s, 3H), 3.61-3.54 (m, 1H), 3.50 (s, 2H), 3.22-3.13 (m, 1H), 3.03-2.96 (m, 1H), 2.70-2.60 (m, 1H), 2.56 (d, J=2.3 Hz, 3H), 1.20 (dd, J=6.8, 14.3 Hz, 6H). MS: calc'd 589 (MH.sup.+), measured 589 (MH.sup.+).

(433) Example 85B was obtained as a light yellow solid (2.3 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.07 (d, J=3.0 Hz, 1H), 7.96 (d, J=3.3 Hz, 1H), 7.25-7.17 (m, 1H), 7.16-7.07 (m, 1H), 5.99 (s, 1H), 4.30 (d, J=16.8 Hz, 1H), 4.16-3.99 (m, 2H), 3.92-3.82 (m, 2H), 3.65 (s, 3H), 3.59-3.53 (m, 1H), 3.51-3.43 (m, 2H), 3.24-3.09 (m, 2H), 2.78-2.68 (m, 1H), 2.55 (d, J=2.3 Hz, 3H), 1.19 (dd, J=6.8, 14.1 Hz, 6H). MS: calc'd 589 (MH.sup.+), measured 589 (MH.sup.+).

(434) Example 85A was synthesized from compound 85A-1 (faster eluting) and Example 85B was synthesized from compound 85B-1 (slower eluting) on ChiralPak AD-3 column eluting with 20% isopropanol (0.05% DEA)/CO.sub.2.

Example 86A and 86B (Separated Two Single Isomers)

(8R,8aS)-2-tert-butyl-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-2-tert-butyl-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(435) ##STR00186##

Preparation of Example 86A and 86B

(436) ##STR00187##

(437) The title two compounds were prepared in analogy to Example 82A and 82B by using (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) instead of ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and methyl 2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 86-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(438) Example 86A was obtained as a light yellow solid (64.8 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98 (d, J=3.0 Hz, 1H), 7.80 (d, J=3.3 Hz, 1H), 7.52-7.45 (m, 1H), 7.24 (dd, J=2.6, 8.7 Hz, 1H), 7.06 (d, J=2.8 Hz, 1H), 6.19 (s, 1H), 4.32 (d, J=17.3 Hz, 1H), 3.97 (br. s., 1H), 3.82-3.72 (m, 2H), 3.65-3.58 (m, 4H), 3.57-3.51 (m, 1H), 3.42-3.36 (m, 1H), 3.19-3.09 (m, 1H), 2.99-2.89 (m, 1H), 2.65-2.53 (m, 1H), 1.41 (s, 9H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

(439) Example 86B was obtained as a light yellow solid (71.1 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.46 (dd, J=6.1, 8.7 Hz, 1H), 7.25 (dd, J=2.6, 8.7 Hz, 1H), 7.13-7.05 (m, 1H), 6.15 (s, 1H), 4.25 (d, J=17.3 Hz, 1H), 4.03 (br. s., 1H), 3.86-3.72 (m, 2H), 3.65-3.57 (m, 4H), 3.55-3.48 (m, 1H), 3.41-3.36 (m, 1H), 3.19-3.05 (m, 2H), 2.75-2.62 (m, 1H), 1.40 (s, 9H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

(440) Example 86A was synthesized from compound 86A-1 (faster eluting) and Example 86B was synthesized from compound 86B-1 (slower eluting) on ChiralPak AD-3 column eluting with 25% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Methyl 2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound 86-C)

(441) Compound 86-C was prepared in analogy to compound 82-C by using tert-butylamine instead of cyclopropylamine.

Example 87A and 87B (Separated Two Single Isomers)

(8R,8aS)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(442) ##STR00188##

Preparation of Example 87A and 87B

(443) ##STR00189##

(444) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl 2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 86-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(445) Compound 87B-1 (Single isomer. Structure is either one of the two structures showing above): Methyl (8S,8aR)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylate; or Methyl (8R,8aS)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylate

(446) Compound 87B-1 was obtained as a light yellow solid (316 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.34-7.23 (m, 2H), 7.16 (t, J=8.5 Hz, 1H), 6.22 (s, 1H), 5.51 (s, 2H), 4.14 (d, J=17.1 Hz, 1H), 4.08-4.00 (m, 2H), 3.89 (s, 1H), 3.81-3.69 (m, 5H), 3.58-3.52 (m, 1H), 3.35 (br. s., 1H), 3.27 (d, J=9.5 Hz, 1H), 3.16-3.04 (m, 1H), 2.95 (d, J=12.3 Hz, 1H), 2.52 (dt, J=3.4, 12.0 Hz, 1H), 1.39 (s, 9H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 633 (MH.sup.+), measured 633 (MH.sup.+)

(447) Example 87A was obtained as a light yellow solid (52.7 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98 (d, J=3.0 Hz, 1H), 7.79 (d, J=3.0 Hz, 1H), 7.35-7.27 (m, 2H), 7.17 (s, 1H), 6.25 (s, 1H), 4.39-4.28 (m, 1H), 4.09-4.01 (m, 2H), 3.99-3.86 (m, 1H), 3.76 (d, J=9.8 Hz, 2H), 3.64-3.58 (m, 1H), 3.57-3.51 (m, 1H), 3.40-3.36 (m, 1H), 3.13 (br. s., 1H), 3.00-2.84 (m, 1H), 2.63-2.47 (m, 1H), 1.41 (s, 9H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

(448) Example 87B was obtained as a light yellow solid (61.3 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.36-7.26 (m, 2H), 7.21-7.14 (m, 1H), 6.22 (s, 1H), 4.27 (d, J=16.8 Hz, 1H), 4.09-3.97 (m, 3H), 3.86-3.71 (m, 2H), 3.65-3.55 (m, 1H), 3.55-3.46 (m, 1H), 3.39-3.34 (m, 1H), 3.20-3.02 (m, 2H), 2.66 (br. s., 1H), 1.40 (s, 9H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+)

(449) Example 87A was synthesized from compound 87A-1 (faster eluting) and Example 87B was synthesized from compound 87B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Example 88A and 88B (Separated Two Single Isomers)

(8R,8aS)-2-tert-butyl-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-2-tert-butyl-7-[[(4S)-4-(3,4-difluoro-2-methyl-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(450) ##STR00190##

Preparation of Example 88A and 88B

(451) ##STR00191##

(452) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-5) instead of ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and methyl 2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 86-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(453) Example 88A was obtained as a light yellow solid (37.2 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.14-7.09 (m, 1H), 7.08-6.99 (m, 1H), 5.94 (s, 1H), 4.32 (d, J=17.1 Hz, 1H), 3.91 (d, J=17.1 Hz, 1H), 3.79-3.71 (m, 2H), 3.66-3.58 (m, 4H), 3.56-3.50 (m, 1H), 3.36 (d, J=9.8 Hz, 1H), 3.17-3.06 (m, 1H), 2.89 (d, J=11.3 Hz, 1H), 2.56 (d, J=2.3 Hz, 4H), 1.40 (s, 9H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

(454) Example 88B was obtained as a light yellow solid (61.2 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.3 Hz, 1H), 7.14-7.02 (m, 2H), 5.91 (s, 1H), 4.25 (d, J=17.1 Hz, 1H), 4.01 (d, J=17.1 Hz, 1H), 3.85-3.72 (m, 2H), 3.66-3.56 (m, 4H), 3.54-3.46 (m, 1H), 3.37 (br. s., 1H), 3.20-3.02 (m, 2H), 2.69 (d, J=10.3 Hz, 1H), 2.56 (d, J=2.5 Hz, 3H), 1.40 (s, 9H). MS: calc'd 603 (MH.sup.+), measured 603 (MH.sup.+).

(455) Example 88A was synthesized from compound 88A-1 (faster eluting) and Example 88B was synthesized from compound 88B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Example 89

Methyl 7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-2,5,6,8-tetrahydro-1H-imidazo[1,5-a]pyrazine-8a-carboxylate

(456) ##STR00192##

Preparation of Example 89

(457) ##STR00193##

(458) The title compound was prepared in analogy to Example 1 by using methyl 3-oxo-1,2,5,6,7,8-hexahydroimidazo[1,5-a]pyrazine-8a-carboxylate TFA salt (Compound X) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D).

Example 90A and 90B (Separated Two Single Isomers)

2-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-1,1-dimethyl-3-oxo-6,8-dihydro-5H-oxazolo[3,4-a]pyrazin-8a-yl]acetic Acid; and

2-[(8aR)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-1,1-dimethyl-3-oxo-6,8-dihydro-5H-oxazolo[3,4-a]pyrazin-8a-yl]acetic Acid

(459) ##STR00194##

Preparation of Example 90A and 90B

(460) ##STR00195##

(461) The title two compounds were prepared in analogy to Example 82A and 82B by using (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) instead of ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and methyl 2-(1,1-dimethyl-3-oxo-5,6,7,8-tetrahydrooxazolo[3,4-a]pyrazin-8a-yl)acetate (compound 90-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(462) Example 90A was obtained as a light yellow solid (13 mg). .sup.1H NMR (400 MHz, MeOD) ppm 7.92 (d, J=3.3 Hz, 1H), 7.71 (d, J=3.0 Hz, 1H), 7.43 (dd, J=6.0, 8.8 Hz, 1H), 7.23 (dd, J=2.5, 8.8 Hz, 1H), 7.06 (dt, J=2.5, 8.4 Hz, 1H), 6.16 (s, 1H), 4.21 (d, J=16.3 Hz, 1H), 3.87-3.71 (m, 3H), 3.61 (s, 3H), 3.52 (d, J=11.5 Hz, 1H), 3.37 (br, 1H), 2.94 (d, J=11.3 Hz, 1H), 2.63 (d, J=16.1 Hz, 1H), 2.46-2.30 (m, 2H), 1.47 (s, 3H), 1.42 (s, 3H). LC/MS: calc'd 592 (MH.sup.+), exp 592 (MH.sup.+).

(463) Example 90B was obtained as a light yellow solid (25 mg). .sup.1H NMR (400 MHz, MeOD) ppm 7.96 (d, J=3.0 Hz, 1H), 7.75 (d, J=3.0 Hz, 1H), 7.54 (dd, J=6.3, 8.8 Hz, 1H), 7.21 (dd, J=2.8, 8.8 Hz, 1H), 7.06 (dt, J=2.5, 8.4 Hz, 1H), 6.16 (s, 1H), 4.09-3.90 (m, 2H), 3.89-3.71 (m, 2H), 3.66-3.55 (m, 4H), 3.36 (br, 1H), 2.82 (d, J=8.3 Hz, 1H), 2.72 (d, J=16.8 Hz, 1H), 2.43 (d, J=11.3 Hz, 1H), 2.31 (dt, J=4.0, 11.9 Hz, 1H), 1.57 (s, 3H), 1.47 (s, 3H); LC/MS: calc'd 592 (MH.sup.+), exp 592 (MH.sup.+).

(464) Example 90A was synthesized from compound 90A-1 (faster eluting) and Example 90B was synthesized from compound 90B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Methyl 2-(1,1-dimethyl-3-oxo-5,6,7,8-tetrahydrooxazolo[3,4-a]pyrazin-8a-yl)acetate (Compound 90-C)

(465) ##STR00196## ##STR00197##
Step 1:

(466) To a solution of DIPA (3.67 g, 36 mmol, 1.2 eq) in THF (18 mL) was added n-BuLi (2M, 18 mL, 1.2 eq) dropwise at 78 C. over 15 min and the mixture was stirred for another 15 min at 78 C. Then compound AH (10.3 g, 30 mmol, 1.0 eq) in THF (30 mL) was added dropwise to the reaction at 78 C., after the addition was completed, the mixture was stirred for another 30 min at 78 C. BOMCl (7 g, 45 mmol) in THF (20 mL) was added dropwise over 20 min at 78 C., the reaction mixture was stirred for 16 h and the temperature was warmed to rt during this period. The reaction was quenched with 1M HCl (50 mL), extracted with EA (100 mL) two times, the organic layer was dried and concentrated, the residue was purified on silica gel (EA/PE: 0%-20%) to give compound 90-C-1 as colorless oil (8.2 g).

(467) Step 2:

(468) A mixture of compound 90-C-1 (8.2 g, 17.6 mmol) and Pd(OH).sub.2/C (500 mg, 20%) in MeOH (100 mL) was stirred at rt for 2 h under hydrogen, then the solid was filtered off, the filtrate was concentrated and the residue was purified on silica gel (EA/PE: 0%-40%) to give compound 90-C-2 as a colorless oil (6.1 g).

(469) Step 3:

(470) To a solution of 90-C-2 (3.74 g, 0.01 mol, 1.0 eq) and imidazole (0.81 g, 0.012 mol, 1.2 eq) in DMF (20 mL) was added TBSCl (1.65 g, 0.11 mol, 1.1 eq) slowly at rt, then the mixture was stirred at rt for 16 h, diluted with EA (50 mL) washed with water (50 mL) and brine (50 mL), the organic layer was dried over Na.sub.2SO.sub.4 and concentrated to give compound 90-C-3 as a colorless oil (4.4 g). MS: calc'd 489 (MH.sup.+), measured 489 (MH.sup.+).

(471) Step 4:

(472) To a solution of 90-C-3 (4.4 g, 9 mmol, 1.0 eq) in THF (50 mL) was added CH.sub.3MgBr (3.2M, 15 mL, 5.0 eq) dropwise at 40 C. over 15 min, then the reaction mixture was stirred for 16 h and the temperature was warmed to rt during this period. The reaction was quenched with 1M HCl (50 mL), extracted with EA (100 mL), the organic layer was dried and concentrated, and residue was purified on silica gel (EA/PE: 20%-40%) to give compound 90-C-4 as colorless oil (1.9 g). MS: calc'd 415 (MH.sup.+), measured 415 (MH.sup.+).

(473) Step 5:

(474) A solution of 90-C-4 (1.86 g, 4.5 mmol, 1.0 eq) and TBAF (1 M, 9 mL, 2.0 eq) in THF (20 mL) was stirred at rt for 2 h, diluted with EA (50 mL), washed with water (50 mL) and brine (50 mL), the organic layer was dried over Na.sub.2SO.sub.4 and concentrated, the residue was purified on silica gel (EA/PE: 30%-70%) to give compound 90-C-5 as colorless oil (1.2 g). MS: calc'd 301 (MH.sup.+), measured 301 (MH.sup.+).

(475) Step 6:

(476) A solution of 90-C-5 (0.9 g, 3 mmol, 1.0 eq), PPh.sub.3 (1.57 g, 6 mmol, 2.0 eq) and imidazole (0.41 g, 6 mmol, 2.0 eq) in THF (20 mL) was refluxed for 1 h, then Iodine (1.3 g, 4.5 mmol, 1.5 eq) was added and refluxed for 2 h. Then reaction diluted with EA (50 mL), washed with water (50 mL) and brine (50 mL), the organic layer was dried (Na.sub.2SO.sub.4) and concentrated, the residue was purified on silica gel (EA/PE: 0%-25%) to give compound 90-C-6 as colorless oil (0.61 g). MS: calc'd 411 (MH.sup.+), measured 411 (MH.sup.+).

(477) Step 7:

(478) A mixture of 90-C-6 (0.61 g, 1.5 mmol, 1.0 eq) and KCN (0.195 g, 3 mmol, 2.0 eq) in DMSO (10 mL) was stirred at 80 C. for 16 h. Then reaction diluted with EA (50 mL), washed with water (50 mL) and brine (50 mL), the organic layer was dried over Na.sub.2SO.sub.4 and concentrated, the residue was purified on silica gel (EA/PE: 0%-25%) to give compound 90-C-7 as colorless oil (0.41 g). MS: calc'd 310 (MH.sup.+), measured 310 (MH.sup.+).

(479) Step 8:

(480) A mixture of 90-C-7 (0.41 g, 1.3 mmol, 1.0 eq) in HCl/MeOH (3M, 10 mL) was stirred at 80 C. for 16 h. Then reaction was concentrated to give compound 90-C as white solid (0.36 g). MS: calc'd 243 (MH.sup.+), measured 243 (MH.sup.+).

Example 91A and 91B (Separated Two Single Isomers)

(8R,8aS)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-2-tert-butyl-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(481) ##STR00198##

Preparation of Example 91A and 91B

(482) ##STR00199##

(483) The title two compounds were prepared in analogy to Example 82A and 82B by using methyl 2-methyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 91-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(484) Example 91A was obtained as a light yellow solid (26 mg). .sup.1H NMR (400 MHz, MeOD) 7.97 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.36-7.26 (m, 2H), 7.22-7.11 (m, 1H), 6.25 (s, 1H), 4.30 (d, J=17.3 Hz, 1H), 4.04 (q, J=7.2 Hz, 2H), 3.95-3.82 (m, 3H), 3.61-3.45 (m, 2H), 3.31-3.11 (m, 2H), 2.93-2.81 (m, 4H), 2.49 (br, 1H), 1.13 (t, J=7.0 Hz, 3H). LC/MS: calc'd 577 (MH+), exp 577 (MH+).

(485) Example 91B was obtained as a light yellow solid (38 mg). .sup.1H NMR (400 MHz, MeOD) 7.96 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.39-7.25 (m, 2H), 7.17 (td, J=2.0, 7.5, 9.1 Hz, 1H), 6.21 (s, 1H), 4.24 (d, J=16.8 Hz, 1H), 4.04 (q, J=7.1 Hz, 2H), 3.98-3.78 (m, 3H), 3.60-3.42 (m, 2H), 3.31-3.16 (m, 2H), 3.04 (d, J=10.3 Hz, 1H), 2.83 (s, 3H), 2.61 (br, 1H), 1.12 (t, J=7.0 Hz, 3H). LC/MS: calc'd 577 (MH+), exp 577 (MH+).

(486) Example 91A was synthesized from compound 91A-1 (faster eluting) and Example 91B was synthesized from compound 91B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Methyl 2-methyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound 91-C)

(487) Compound 91-C was prepared in analogy to compound 82-C by using methyl amine instead of cyclopropylamine.

Example 92A and 92B (Separated Two Single Isomers)

Methyl (4R)-6-[[(8R,8aS)-2-tert-butyl-8-carbamoyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate; and

Methyl (4R)-6-[[(8S,8aR)-2-tert-butyl-8-carbamoyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(488) ##STR00200##

Preparation of Example 92A and 92B

(489) ##STR00201##

(490) The title two compounds were prepared in analogy to Example 82A and 82B by using trans-2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxamide (compound 92-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C). The crude product was purified by HPLC to give a mixture of two isomers which were further resolved by SFC to give two single isomers: 92A (faster eluting) and 92B (slower eluting) with 30% isopropanol (0.05% DEA)/CO.sub.2 on ChiralPak AD-3 column.

(491) Example 92A was obtained as a light yellow solid (30 mg). .sup.1H NMR (400 MHz, MeOD) =7.97 (d, J=3.0 Hz, 1H), 7.74 (d, J=3.3 Hz, 1H), 7.37-7.21 (m, 2H), 7.20-7.08 (m, 1H), 6.20 (s, 1H), 4.18 (d, J=17.1 Hz, 1H), 4.02 (q, J=7.0 Hz, 2H), 3.84-3.72 (m, 3H), 3.55 (t, J=8.8 Hz, 2H), 3.14 (dt, J=3.5, 12.7 Hz, 1H), 3.05 (d, J=9.3 Hz, 1H), 3.00-2.89 (m, 1H), 2.45 (dt, J=3.5, 11.9 Hz, 1H), 1.40 (s, 9H), 1.12 (t, J=7.2 Hz, 3H). MS: calc'd 618 (MH.sup.+), exp 618 (MH.sup.+).

(492) Example 92B was obtained as a light yellow solid (30 mg). .sup.1H NMR (400 MHz, MeOD) =7.98 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.36-7.23 (m, 2H), 7.20-7.09 (m, 1H), 6.25 (s, 1H), 4.24 (d, J=17.3 Hz, 1H), 4.04 (q, J=7.1 Hz, 2H), 3.85-3.68 (m, 3H), 3.57 (t, J=8.8 Hz, 1H), 3.37-2.35 (m, 1H), 3.20-2.99 (m, 2H), 2.85-2.69 (m, 1H), 2.32 (dt, J=3.4, 12.0 Hz, 1H), 1.49-1.34 (s, 9H), 1.13 (t, J=7.0 Hz, 3H). MS: calc'd 618 (MH.sup.+), exp 618 (MH.sup.+).

Preparation of trans-2-tert-butyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxamide (Compound 92-C)

(493) ##STR00202##
Step 1:

(494) To a solution of compound 92-C-1 (355 mg, 1 mmol) in THF (5 mL) was added lithium hydroxide monohydrate (124 mg, 3 mmol) in water (1 mL). After the reaction mixture was stirred at 35 C. for 2 hours, it was neutralized with 1N hydrochloride solution to pH 3.0. The mixture was extracted with ethyl acetate (30 mL) three times. The combined organic phase was dried over Na.sub.2SO.sub.4, filtrated and then concentrated to give compound 92-C-2 as a white solid (340 mg). MS: calc'd 356 (MH.sup.+), exp 356 (MH.sup.+).

(495) Step 2:

(496) A mixture of compound 92-C-2 (340 mg, 1 mmol), HATU (380 mg, 1 mmol), NH.sub.4Cl (275 mg, 5 mmol) and DIPEA (775 mg, 6 mmol) in THF (10 mL) was stirred 50 C. for 2 hours. Then the undissolved material was filtered off and the filtrate was concentrated. The residue was purified on silica gel (EA/PE: 0%-40%) to give compound 92-C-3 as a white solid (200 mg). MS: calc'd 355 (MH.sup.+), exp 355 (MH.sup.+).

(497) Step 3:

(498) A mixture of compound 92-C-3 (200 mg, 0.6 mmol) in DCM/TFA (5 mL, 2:1) was stirred at room temperature for 2 hours, then the mixture was concentrated under reduced pressure to give compound 92-C as a slight yellow oil (200 mg), which was used directly in the next step. MS: calc'd 255 (MH.sup.+), exp 255 (MH.sup.+).

Preparation of trans-7-tert-butyl-8-methyl 2-tert-butyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7,8-dicarboxylate (Compound 92-C-1)

(499) Compound 92-C-1 was prepared in analogy to compound 82-C-5 by using tert-butylamine instead of cyclopropylamine.

Example 93

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-propoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(500) ##STR00203##

Preparation of Example 93

(501) ##STR00204##

(502) The title compound was prepared in analogy to Example 1 by using 3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-propoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (compound C-6) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 93 was obtained as a light yellow solid (6 mg). .sup.1H NMR (400 MHz, MeOD) =7.96 (d, J=3.0 Hz, 1H), 7.75 (d, J=3.0 Hz, 1H), 7.34-7.21 (m, 2H), 7.15 (dt, J=1.6, 8.5 Hz, 1H), 6.24 (s, 1H), 4.19-4.06 (m, 1H), 4.03-3.81 (m, 5H), 3.51 (t, J=8.9 Hz, 1H), 3.45-3.37 (m, 1H), 3.33-3.29 (m, 1H), 3.25-3.15 (m, 1H), 3.11 (dd, J=4.0, 9.5 Hz, 1H), 2.97-2.78 (m, 2H), 2.41-2.29 (m, 1H), 2.19 (t, J=10.9 Hz, 1H), 1.59-1.47 (m, 2H), 1.21 (d, J=2.8 Hz, 6H), 0.76 (t J=7.4 Hz, 3H). MS: calc'd 633 (MH.sup.+), measured 633 (MH.sup.+).

Preparation of Propyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-6)

(503) Compound C-6 was prepared in analogy to compound C by using n-propyl acetoacetate instead of methyl acetoacetate.

Example 94

4-[(8aS)-7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-butanoic Acid

(504) ##STR00205##

Preparation of Example 94

(505) ##STR00206##

(506) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-1) and (3R)-3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]butanoic acid TFA salt (Compound 54-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 57 was obtained as a light yellow solid (48 mg). .sup.1H NMR (MeOD, 400 MHz): =7.97 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.02-7.39 (m, 3H), 6.24 (s, 1H), 3.99-4.16 (m, 2H), 3.73-3.95 (m, 3H), 3.47 (t, J=9.0 Hz, 2H), 3.02-3.25 (m, 3H), 2.68-2.97 (m, 2H), 2.10-2.48 (m, 3H), 1.76 (br. s., 2H), 1.23 (s, 6H), 1.13 ppm (t, J=7.2 Hz, 3H). MS: calc'd 633 (MH.sup.+), measured 633 (MH.sup.+).

Example 95

5-[7-[[(4R)-4-(2-chloro-4-fluoro-phenyl)-5-methoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]pyridine-2-carboxylic Acid

(507) ##STR00207##

Preparation of Example 95

(508) ##STR00208##

(509) The title compound was prepared in analogy to Example 1 by using 5-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)pyridine-2-carboxylic acid TFA salt (Compound 95-A) instead of hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 95 was obtained as a light yellow solid (48 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 9.02 (s, 1H), 8.17 (s, 2H), 8.01 (s, 1H), 7.88 (s, 1H), 7.51-7.54 (m, 1H), 7.30-7.28 (m, 1H), 7.10-7.13 (m, 1H), 6.20 (s, 1H), 4.41-4.63 (m, 2H), 4.17-4.35 (m, 3H), 3.51-3.75 (m, 7H), 3.01-3.12 (m, 2H). MS: calc'd 626 (MH.sup.+), measured 626 (MH.sup.+).

Preparation of 5-(3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl)pyridine-2-carboxylic Acid (Compound 95-A)

(510) ##STR00209##
Step 1:

(511) A solution of 5-bromopyridine-2-carboxylic acid (5.0 g, 24.75 mmol) in MeOH (30 mL) was added thionyl chloride (10 mL) dropwise at 0 C. Then the mixture was stirred for 12 hours at 60 C. After removal of solvent and the residual thionyl chloride, the crude product methyl 5-bromopyridine-2-carboxylate was obtained and used in the next step without further purification.

(512) Step 2:

(513) A mixture of methyl 5-bromopyridine-2-carboxylate (compound 95-A-1, 0.72 g, 3.28 mmol), tert-butyl 3-oxo-1,2,5,6,8,8a-hexahydroimidazo[1,5-a]pyrazine-7-carboxylate (racemic compound M, 0.40 g, 1.64 mmol), bis(dibenzylideneacetone) palladium (94.4 mg, 0.164 mmol), ()-2,2-bis(diphenylphosphino)-1,1-binaphthyl (196.8 mg, 0.33 mmol), cesium carbonate (160.4 mg, 4.92 mmol) in dioxane (3 mL) was stirred for 3 hours at 130 C. in microwave. After removal of solvent, the residue was purified by column to afford product 95-A-2 as white solid. MS: calc'd 377 (MH.sup.+), measured 377 (MH.sup.+).

(514) Step 3:

(515) A mixture of compound 95-A-2 (0.24 g, 0.64 mmol), lithium hydroxide (0.13 g, 3.2 mmol) in water (2 mL) and MeOH (5 mL) was stirred for 12 hours at room temperature. The reaction mixture was then neutralized with 1 N HCl to pH 6, extracted with ethyl acetate. The organic layer was dried over sodium sulfate. After removal of solvent, the residue was treated with TFA (2 mL) in DCM (10 mL). After the reaction mixture was stirred for 30 minutes, the solvent was removed to give the crude product, which was used in the next step directly. MS: calc'd 263 (MH.sup.+), measured 263 (MH.sup.+).

Example 96

(S)-6-[(S)-2-(2-Carboxy-2,2-difluoro-ethyl)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-7-ylmethyl]-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic Acid Ethyl Ester

(516) ##STR00210##

Preparation of Example 96

(517) ##STR00211##

(518) The title compound was prepared in analogy to Example 1 by using ethyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (compound C-7) and 2,2-difluoro-3-((S)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-2-yl)-propionic acid trifluoroacetate salt (Compound 96-A) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 96 was obtained as a light yellow solid (15 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 8.01 (d, 1H), 7.90 (d, 1H), 7.26 (m, 1H), 7.07-7.15 (m, 1H), 5.94-5.98 (m, 1H), 4.69 (d, 1H), 4.51 (d, 1H), 4.05-4.20 (m, 4H), 3.86-3.98 (m, 2H), 3.62-3.80 (m, 4H), 3.41-3.52 (m, 1H), 3.07-3.22 (m, 3H), 2.52 (d, 3H), 1.15 (m, 3H). MS: calc'd 625 (MH.sup.+), measured 625 (MH.sup.+).

Preparation of 2,2-difluoro-3-((S)-3-oxo-hexahydro-imidazo[1,5-a]pyrazin-2-yl)-propionic Acid Trifluoroacetate Salt (Compound 96-A)

(519) ##STR00212##
Step 1.

(520) To a stirred solution of 3-amino-2,2-difluoro-propionic acid hydrochloride salt (400 mg, 1.10 mmol) in DCM (5 mL) was added DIPEA (142 mg, 1.10 mmol) at 0 C., sequentially followed by a solution of compound S (350 mg, 1 mmol) in DCM (5 mL). The reaction mixture was stirred at 0 C. for five minutes. Then NaBH(OAc).sub.3 was added to the mixture. The resulting mixture was stirred at rt overnight. The reaction mixture was partitioned between DCM and water. The organic layer was separated, dried over Na.sub.2SO.sub.4, concentrated in vacuum and isolated to give compound 96-A-1 (320 mg).

(521) Step 2.

(522) A mixture of compound 96-A-1 (320 mg, 0.70 mmol) and Pd/C (30 mg) in MeOH (5 mL) was stirred with a hydrogen balloon at room temperature overnight. The reaction mixture was filtered and the filtrate was concentrated. The crude product 96-A-2 was obtained (226 mg) and used in the next step without further purification.

(523) Step 3.

(524) To a solution of compound 96-A-2 (crude 226 mg, 0.70 mmol) in dichloromethane (3 ml) was added DIPEA (0.6 mL, 3.50 mmol) at 0 C., then triphosgene (103 mg, 0.35 mmol) was added to the reaction mixture. The resulting mixture was warmed to rt and stirred overnight. The reaction mixture was concentrated and the residue was directly used in the next step. The amount of crude product 96-A-3 was 340 mg.

(525) Step 4.

(526) To a solution of compound 96-A-3 (crude 340 mg, 0.70 mmol) in dichloromethane (2 mL) was added trifluoroacetic acid (5 mL) at room temperature. After the reaction mixture was stirred for 1.5 hours, the solvent was removed to give the crude product 96-A (260 mg) which was used directly in the next step.

Preparation of Ethyl (4S)-6-(bromomethyl)-4-(3,4-difluoro-2-methyl-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate (Compound C-7)

(527) Compound C-7 was prepared in analogy to compound C by using 3,4-difluoro-2-methylbenzaldehyde and ethyl acetoacetate instead of 2-chloro-4-fluorobenzaldehyde and methyl acetoacetate.

Example 97A and 97B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-(cyclopropylmethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-(cyclopropylmethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(528) ##STR00213##

Preparation of Example 97A and 97B

(529) ##STR00214##

(530) The title two compounds were prepared in analogy to Example 82A and 82B by using trans-methyl 2-(cyclopropylmethyl)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 97-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(531) Example 97A was obtained as a light yellow solid (25 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98 (d, J=3.0 Hz, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.35-7.26 (m, 2H), 7.20-7.11 (m, 1H), 6.25 (s, 1H), 4.30 (s, 1H), 4.10-3.99 (m, 2H), 3.95-3.86 (m, 2H), 3.81 (d, J=11.5 Hz, 1H), 3.68 (d, J=8.0 Hz, 1H), 3.65-3.58 (m, 1H), 3.37-3.34 (m, 2H), 3.25-3.16 (m, 1H), 3.25-3.16 (m, 1H), 3.15-3.07 (m, 2H), 2.91 (d, J=11.5 Hz, 1H), 2.60-2.49 (m, 1H), 1.13 (t, J=7.2 Hz, 3H), 1.03-0.93 (m, 1H), 0.61-0.53 (m, 2H), 0.28-0.23 (m, 2H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

(532) Example 97B was obtained as a light yellow solid (19 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.33-7.23 (m, 3H), 7.15 (t, J=8.3 Hz, 1H), 6.22 (s, 1H), 4.12-4.02 (m, 3H), 3.95-3.84 (m, 3H), 3.52 (t, J=8.9 Hz, 1H), 3.44-3.38 (m, 1H), 3.29 (s, 1H), 3.24-3.09 (m, 2H), 2.93 (d, J=11.0 Hz, 1H), 2.84 (d, J=8.5 Hz, 1H), 2.48 (d, J=0.8 Hz, 3H), 2.37 (dt, J=3.4, 11.6 Hz, 1H), 2.18 (t, J=10.9 Hz, 1H), 1.21 (d, J=3.0 Hz, 6H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 617 (MH.sup.+), measured 617 (MH.sup.+).

(533) Example 97A was synthesized from compound 97A-1 (faster eluting) and Example 97B was synthesized from compound 97B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of trans-methyl 2-(cyclopropylmethyl)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound 97-C)

(534) Compound 97-C was prepared in analogy to compound 82-C by using cyclopropylmethanamine instead of cyclopropylamine.

Example 98

3-[(8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-(4-methylthiazol-2-yl)-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic Acid

(535) ##STR00215##

Preparation of Example 93

(536) ##STR00216##

(537) The title compound was prepared in analogy to Example 1 by using ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-(4-methylthiazol-2-yl)-1,4-dihydropyrimidine-5-carboxylate (compound C-8) and 3-[(8aS)-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-2-yl]-2,2-dimethyl-propanoic acid (Compound Q) instead of (R)-6-bromomethyl-4-(2-chloro-4-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydro-pyrimidine-5-carboxylic acid methyl ester (Compound C) and hexahydro-pyrazino[1,2-c][1,3]oxazin-6-one (Compound D). Example 93 was obtained as a light yellow solid (60 mg). .sup.1H NMR (400 MHz, MeOD) =7.33-7.23 (m, 3H), 7.15 (t, J=8.3 Hz, 1H), 6.22 (s, 1H), 4.12-4.02 (m, 3H), 3.95-3.84 (m, 3H), 3.52 (t, J=8.9 Hz, 1H), 3.44-3.38 (m, 1H), 3.29 (s, 1H), 3.24-3.09 (m, 2H), 2.93 (d, J=11.0 Hz, 1H), 2.84 (d, J=8.5 Hz, 1H), 2.48 (d, J=0.8 Hz, 3H), 2.37 (dt, J=3.4, 11.6 Hz, 1H), 2.18 (t, J=10.9 Hz, 1H), 1.21 (d, J=3.0 Hz, 6H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 633 (MH.sup.+), measured 633 (MH.sup.+).

Preparation of Ethyl (4R)-6-(bromomethyl)-4-(2-chloro-3-fluoro-phenyl)-2-(4-methylthiazol-2-yl)-1,4-dihydropyrimidine-5-carboxylate (Compound C-8)

(538) Compound C-8 was prepared in analogy to compound C by using 4-methylthiazole-2-carbonitrile and ethyl acetoacetate instead of thiazole-2-carbonitrile and methyl acetoacetate.

Example 99

2-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-6-oxo-1,3,4,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1-carboxylic Acid (Mixture of Two Isomers)

(539) ##STR00217##

Preparation of Example 99

(540) ##STR00218##

(541) The title two compounds were prepared in analogy to Example 82A and 82B by using trans-6-oxo-2,3,4,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazine-1-carboxylic acid (Compound 99-A) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C) without chiral separation of two trans isomers. Example 99 was obtained as a mixture of two isomers (light yellow solid, 79 mg). .sup.1H NMR (400 MHz, MeOD) =7.95 (d, J=3.0 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 7.35-7.26 (m, 2H), 7.17 (t, J=8.2 Hz, 1H), 6.22 (s, 1H), 4.25 (d, J=17.1 Hz, 1H), 4.08-4.01 (m, 3H), 3.98 (s, 2H), 3.20-3.09 (m, 3H), 2.61 (t, J=12.7 Hz, 1H), 2.52-2.42 (m, 2H), 2.34-2.23 (m, 1H), 2.08-1.97 (m, 1H), 1.12 (t, J=7.2 Hz, 3H). MS: calc'd 562 (MH.sup.+), measured 562 (MH.sup.+).

Preparation of trans-6-oxo-2,3,4,7,8,8a-hexahydro-1H-pyrrolo[1,2-a]pyrazine-1-carboxylic Acid (Compound 99-A)

(542) ##STR00219##
Step 1:

(543) To a stirred solution of compound 82-C-2 (12 g, 32.3 mmol) in DCM (100 mL) was added TEA (6.6 g, 64.5 mmol) and ethyl (triphenylphosphoranylidene)acetate (12.3 g, 35.5 mmol). The mixture was stirred at 25 C. for 15 h. Water (100 mL) was added, and the mixture was extracted with DCM (100 mL). The organic layer was separated and dried over Na.sub.2SO.sub.4. The solvent was removed and the residue was purified by column to give compound 99-A-1 (10 g). .sup.1H NMR (400 MHz, CDCl.sub.3) =4.90-4.76 (m, 1H), 4.71-4.48 (m, 1H), 4.24-4.08 (m, 2H), 4.03-3.90 (m, 1H), 3.85-3.71 (m, 5H), 3.21-2.87 (m, 2H), 2.31 (dq, J=8.5, 16.5 Hz, 2H), 2.22-2.05 (m, 1H), 1.98-1.80 (m, 1H), 1.57-1.40 (m, 21H), 1.33-1.23 (m, 3H).

(544) Step 2:

(545) To a stirred solution of compound 99-A-1 (20 g, 45.2 mmol) in MeOH (600 mL) was added Pd/C (10 g). The mixture was stirred at 30 C. (50 Psi) for 15 h. The mixture was filtered and the solvent was removed in vacuum to give crude product, which was purified by column to give compound 99-A-2 (17 g, crude).

(546) Step 3:

(547) To a stirred solution of compound 99-A-2 (17 g, 38.2 mmol) in anhydrous DCM (90 mL) was added TFA (180 mL). After the reaction mixture was stirred for 3 hours, the solvent was removed in vacuum to give crude product 99-A-3 (19 g, crude).

(548) Step 4:

(549) To a stirred solution of compound 99-A-3 (11 g, 25.1 mmol) in anhydrous DCM (250 mL) was added DIPEA (16 g, 125.6 mmol). After the mixture was refluxed for 4 hours, the solvent was removed to give the crude product 99-A (7 g), which was used directly in the next step.

Example 101A and 101B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isobutyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-isobutyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(550) ##STR00220##

Preparation of Example 101A and 101B

(551) ##STR00221##

(552) The title two compounds were prepared in analogy to Example 82A and 82B by using trans-methyl 2-isobutyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 101-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(553) Example 101A was obtained as a light yellow solid (10 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98 (d, J=3.3 Hz, 1H), 7.78 (d, J=3.3 Hz, 1H), 7.34-7.27 (m, 2H), 7.19-7.13 (m, 1H), 6.25 (s, 1H), 4.31 (d, J=17.1 Hz, 1H), 4.09-4.01 (m, 2H), 3.94-3.86 (m, 2H), 3.83-3.76 (m, 1H), 3.60-3.48 (m, 2H), 3.29 (d, J=9.5 Hz, 1H), 3.25-3.16 (m, 1H), 3.10-2.98 (m, 2H), 2.90 (d, J=11.0 Hz, 1H), 2.57-2.45 (m, 1H), 1.99-1.88 (m, 1H), 1.13 (t, J=7.0 Hz, 3H), 0.95 (dd, J=4.9, 6.7 Hz, 6H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

(554) Example 101B was obtained as a light yellow solid (14 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.36-7.27 (m, 2H), 7.19-7.14 (m, 1H), 6.21 (s, 1H), 4.24 (d, J=16.8 Hz, 1H), 4.07-3.93 (m, 3H), 3.92-3.82 (m, 2H), 3.59-3.45 (m, 2H), 3.29-3.18 (m, 2H), 3.03 (dd, J=7.5, 11.0 Hz, 3H), 2.66-2.56 (m, 1H), 1.98-1.88 (m, 1H), 1.12 (t, J=7.0 Hz, 3H), 0.94 (d, J=6.8 Hz, 6H). MS: calc'd 619 (MH.sup.+), measured 619 (MH.sup.+).

(555) Example 101A was synthesized from compound 101A-1 (faster eluting) and Example 101B was synthesized from compound 101B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Trans-Methyl 2-isobutyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (Compound 101-C)

(556) Compound 101-C was prepared in analogy to compound 82-C by using 2-methylpropan-1-amine instead of cyclopropylamine.

Example 102A and 102B (Separated Two Single Isomers)

(8R,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-oxazolo[3,4-a]pyrazine-8-carboxylic Acid; and

(8S,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-3-oxo-5,6,8,8a-tetrahydro-1H-oxazolo[3,4-a]pyrazine-8-carboxylic Acid

(557) ##STR00222##

Preparation of Example 102A and 102B

(558) ##STR00223##

(559) The title two compounds were prepared in analogy to Example 82A and 82B by using trans-methyl 3-oxo-1,5,6,7,8,8a-hexahydrooxazolo[3,4-a]pyrazine-8-carboxylate (compound 102-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(560) Example 102A was obtained as a light yellow solid (29 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.78 (d, J=3.0 Hz, 1H), 7.35-7.27 (m, 2H), 7.20-7.13 (m, 1H), 6.26 (s, 1H), 4.63-4.53 (m, 1H), 4.44 (dd, J=6.0, 9.3 Hz, 1H), 4.28 (d, J=17.1 Hz, 1H), 4.15-3.97 (m, 4H), 3.77 (dd, J=2.3, 13.3 Hz, 1H), 3.47 (d, J=9.8 Hz, 1H), 3.30 (d, J=3.5 Hz, 1H), 2.96 (dd, J=2.1, 12.2 Hz, 1H), 2.68-2.58 (m, 1H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 564 (MH.sup.+), measured 564 (MH.sup.+).

(561) Example 102B was obtained as a light yellow solid (27 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.35-7.28 (m, 2H), 7.20-7.14 (m, 1H), 6.23 (s, 1H), 4.61-4.53 (m, 1H), 4.40 (dd, J=6.3, 9.3 Hz, 1H), 4.23-4.17 (m, 1H), 4.13-4.02 (m, 4H), 3.80 (dd, J=2.5, 13.6 Hz, 1H), 3.46 (d, J=9.8 Hz, 1H), 3.39-3.27 (m, 1H), 3.08 (dd, J=2.3, 12.3 Hz, 1H), 2.73 (dt, J=3.6, 12.2 Hz, 1H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 564 (MH.sup.+), measured 564 (MH.sup.+).

(562) Example 102A was synthesized from compound 102A-1 (faster eluting) and Example 102B was synthesized from compound 102B-1 (slower eluting) on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2.

Preparation of Trans-Methyl 3-oxo-1,5,6,7,8,8a-hexahydrooxazolo[3,4-a]pyrazine-8-carboxylate (Compound 102-C)

(563) ##STR00224##
Step I:

(564) To a stirred solution of compound 82-C-1 (8.6 g, 23 mmol) and pyridine (3.64 g, 46 mmol) in DCM (80 mL) was added a solution of (4-nitrophenyl)carbonochloridate (6.95 g, 34.5 mmol) in DCM (20 mL) at 0 C. Then the mixture was warmed to rt and stirred for about 2-3 hrs. The solvent was diluted with DCM (100 mL) and the mixture was washed successively with 0.6N HCl (50 mL), aq. NaHCO.sub.3 (50 mL) and brine. The organic layer was dried over Na.sub.2SO.sub.4, filtered and concentrated in vacuum. The obtained crude product was purified by column chromatography on silica to get the title product 102-C-1 (9.5 g). .sup.1H NMR (400 MHz, CDCl.sub.3) d ppm 8.30 (d, J=8.8 Hz, 2H), 7.43 (t, J=8.5 Hz, 2H), 5.30-5.09 (m, 1H), 4.88-4.71 (m, 1H), 4.54-4.23 (m, 2H), 4.08-3.93 (m, 1H), 3.91-3.77 (m, 5H), 3.33-2.97 (m, 1H), 3.33-2.97 (m, 1H), 1.51-1.47 (t, 18H)

(565) Step II:

(566) To a solution of compound 102-C-1 (9.5 g, 17.6 mmol) in DCM (70 mL) was added TFA (70 mL) at room temperature. Then the mixture was stirred for about 3 h. The solvent was removed under reduced pressure to give the crude product 102-C-2 (10 g crude) as TFA salt which was used in the next step without further purification.

(567) Step III:

(568) To a stirred solution of compound 102-C-2 (crude, 10 g, 17.6 mmol) in DCM (160 mL) was added DIPEA (28 mL) at room temperature. Then the mixture was heated to reflux and stirred for about 4 h. The crude product was purified by column chromatography on silica to give the title compound 102-C (2 g). MS: calc'd 201 (MH.sup.+), measured 201 (MH.sup.+).

Example 103A and 103B (Separated Two Single Isomers)

Ethyl (4R)-6-[[(8R,8aS)-2-tert-butyl-8-(hydroxymethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate; and

Ethyl (4R)-6-[[(8S,8aR)-2-tert-butyl-8-(hydroxymethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(569) ##STR00225##

Preparation of Example 103A and 103B

(570) ##STR00226##

(571) The title two compounds were prepared in analogy to Example 82A-1 and 82B-1 by using trans-2-tert-butyl-8-(hydroxymethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (compound 103-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(572) Example 103A (faster eluting on ChiralPak AD-3 column with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (43 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.95 (d, J=3.0 Hz, 1H), 7.74 (d, J=3.0 Hz, 1H), 7.35-7.26 (m, 2H), 7.19-7.11 (m, 1H), 6.25 (s, 1H), 4.43 (d, J=17.6 Hz, 1H), 4.13-4.01 (m, 3H), 3.97-3.84 (m, 1H), 3.75 (dd, J=4.0, 12.5 Hz, 1H), 3.69-3.60 (m, 2H), 3.58-3.51 (m, 1H), 3.28 (dd, J=7.2, 8.7 Hz, 1H), 3.01 (dt, J=3.1, 12.4 Hz, 1H), 2.81-2.71 (m, 1H), 2.53-2.41 (m, 2H), 1.39 (s, 9H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

(573) Example 103B (slower eluting on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (25 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.98-7.92 (m, 1H), 7.77-7.70 (m, 1H), 7.33-7.23 (m, 2H), 7.19-7.11 (m, 1H), 6.24 (s, 1H), 4.53 (d, J=17.8 Hz, 1H), 4.08-3.95 (m, 3H), 3.74-3.62 (m, 4H), 3.57-3.47 (m, 1H), 3.26 (dd, J=7.4, 8.7 Hz, 1H), 3.07-2.99 (m, 1H), 2.94 (d, J=11.8 Hz, 1H), 2.61 (dt, J=3.5, 11.8 Hz, 1H), 2.50-2.40 (m, 1H), 1.39 (s, 9H), 1.16-1.10 (m, 3H). MS: calc'd 605 (MH.sup.+), measured 605 (MH.sup.+).

Preparation of Trans-Methyl 3-oxo-1,5,6,7,8,8a-hexahydrooxazolo[3,4-a]pyrazine-8-carboxylate (Compound 102-C)

(574) ##STR00227##
Step I:

(575) LiOH.H.sub.2O (1.26 g, 30 mmol) in H.sub.2O (10 mL) was added to a solution of compound 92-C-1 (3.55 g, 10 mmol) in methanol (40 mL) at rt. After the mixture was stirred at rt for 5 h, methanol was removed under reduced pressure and the mixture was adjusted to pH=5 with 2N HCl solution. The mixture was extracted with ethyl acetate (50 mL, 3 times). The combined organic phase was dried and concentrated to afford crude product 103-C-1 (3.14 g) which was used in the next step without further purification. MS: calc'd 342 (MH.sup.+), measured 342 (MH.sup.+).

(576) Step II:

(577) BH.sub.3.THF (1M in THF, 30 mL) was added to a solution of compound 103-C-1 (3 g, 8.8 mmol) in THF (20 mL) at rt. After the reaction mixture was refluxed for 3 hours, it was cooled to 0 C. Methanol was added slowly to previous reaction mixture to quench the reaction. The solvent was removed and the residue was purified by column chromatography to afford product 103-C-2 (2.47 g). MS: calc'd 328 (MH.sup.+), measured 328 (MH.sup.+).

(578) Step III:

(579) TFA (2 mL) was added to a stirring solution of compound 103-C-2 (2 g, 6.1 mmol) in CH.sub.2Cl.sub.2 (20 mL) at rt. After 3 hours, LC-MS indicated that the start materials were consumed. The solvent was removed to give the crude product 103-C which was used in the next step without further purification. MS: calc'd 228 (MH.sup.+), measured 228 (MH.sup.+).

Example 104A and 104B (Separated Two Single Isomers)

Ethyl (4R)-6-[[(8aR)-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate; and

Ethyl (4R)-6-[[(8aS)-2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(580) ##STR00228##

Preparation of Example 104A and 104B

(581) ##STR00229##

(582) The title two compounds were prepared in analogy to Example 82A-1 and 82B-1 by using 2-isopropyl-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (compound 104-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(583) Example 104A (faster eluting on ChiralPak AD-3 column with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (71 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.3 Hz, 1H), 7.75 (d, J=3.0 Hz, 1H), 7.32-7.24 (m, 2H), 7.15 (t, J=8.3 Hz, 1H), 6.24 (s, 1H), 4.12-4.01 (m, 4H), 3.94-3.85 (m, 2H), 3.80 (dd, J=2.0, 13.3 Hz, 1H), 3.51 (t, J=8.9 Hz, 1H), 3.18-3.04 (m, 2H), 2.99 (dd, J=2.8, 10.8 Hz, 1H), 2.79-2.67 (m, 1H), 2.29 (t, J=10.9 Hz, 1H), 2.25-2.17 (m, 1H), 1.20-1.10 (m, 9H). MS: calc'd 561 (MH.sup.+), measured 561 (MH.sup.+).

(584) Example 103B (slower eluting on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (77 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.1 Hz, 1H), 7.75 (d, J=3.3 Hz, 1H), 7.33-7.23 (m, 2H), 7.15 (t, J=8.5 Hz, 1H), 6.24 (s, 1H), 4.13-4.00 (m, 4H), 3.94-3.83 (m, 3H), 3.45 (t, J=8.9 Hz, 1H), 3.22-3.12 (m, 1H), 3.00 (dd, J=4.0, 9.3 Hz, 1H), 2.93-2.80 (m, 2H), 2.34 (dt, J=3.4, 11.6 Hz, 1H), 2.15 (t, J=10.9 Hz, 1H), 1.18-1.09 (m, 9H). MS: calc'd 561 (MH.sup.+), measured 561 (MH.sup.+).

Preparation of 2-isopropyl-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 104-C)

(585) ##STR00230##

(586) tert-Butyl 2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (compound 104-C-1) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. After the reaction mixture was stirred at rt for 1 hour, the solvent was removed in vacuum to give the crude product 104-C, which was used directly in the next step.

Preparation of tert-Butyl 2-isopropyl-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 104-C-1)

(587) Compound 104-C-1 was prepared in analogy to Compound 67-D by using iso-propyl amine instead of 2-aminoethanol.

Example 105A and 105B (Separated Two Single Isomers)

(8R,8aS)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-(2,2-difluoroethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid; and

(8S,8aR)-7-[[(4R)-4-(2-chloro-3-fluoro-phenyl)-5-ethoxycarbonyl-2-thiazol-2-yl-1,4-dihydropyrimidin-6-yl]methyl]-2-(2,2-difluoroethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-8-carboxylic Acid

(588) ##STR00231##

Preparation of Example 105A and 105B

(589) ##STR00232##

(590) The title two compounds were prepared in analogy to Example 82A-1 and 82B-1 by using 2-(2,2-difluoroethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (compound 105-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(591) Example 105A (faster eluting on ChiralPak AD-3 column with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (22 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.77 (d, J=3.0 Hz, 1H), 7.34-7.26 (m, 2H), 7.19-7.12 (m, 1H), 6.25 (s, 1H), 6.17-5.83 (m, 1H), 4.31 (d, J=17.1 Hz, 1H), 4.08-4.00 (m, 2H), 3.96-3.86 (m, 2H), 3.80 (dd, J=2.0, 13.6 Hz, 1H), 3.74-3.68 (m, 1H), 3.66-3.56 (m, 3H), 3.28-3.16 (m, 1H), 2.95-2.87 (m, 2H), 2.53 (dt, J=3.4, 12.0 Hz, 1H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 627 (MH.sup.+), measured 627 (MH.sup.+).

(592) Example 105B (slower eluting on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (22 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.0 Hz, 1H), 7.35-7.27 (m, 2H), 7.17 (t, J=8.1 Hz, 1H), 6.21 (s, 1H), 6.16-5.83 (m, 1H), 4.23 (d, J=16.6 Hz, 1H), 4.06-4.00 (m, 2H), 3.98-3.81 (m, 3H), 3.72-3.66 (m, 1H), 3.65-3.55 (m, 3H), 3.29-3.17 (m, 2H), 3.04 (d, J=10.3 Hz, 1H), 2.66-2.54 (m, 1H), 1.12 (t, J=7.2 Hz, 3H). MS: calc'd 627 (MH.sup.+), measured 627 (MH.sup.+).

Preparation of 2-(2,2-difluoroethyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (compound 105-C)

(593) ##STR00233##

(594) tert-Butyl 2-(2,2-difluoroethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (compound 105-C-1) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. After the reaction mixture was stirred at rt for 1 hour, the solvent was removed in vacuum to give the crude product 105-C, which was used directly in the next step.

Preparation of Tert-Butyl 2-(2,2-difluoroethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 105-C-1)

(595) Compound 105-C-1 was prepared in analogy to Compound 67-D by using 2,2-difluoroethanamine instead of 2-aminoethanol.

Example 106A and 106B (Separated Two Single Isomers)

Ethyl (4R)-6-[[(8aR)-2-(2-hydroxy-2-methyl-propyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate; and

Ethyl (4R)-6-[[(8aS)-2-(2-hydroxy-2-methyl-propyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazin-7-yl]methyl]-4-(2-chloro-3-fluoro-phenyl)-2-thiazol-2-yl-1,4-dihydropyrimidine-5-carboxylate

(596) ##STR00234##

Preparation of Example 106A and 106B

(597) ##STR00235##

(598) The title two compounds were prepared in analogy to Example 82A-1 and 82B-1 by using 2-(2-hydroxy-2-methyl-propyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (compound 106-C) instead of trans-methyl 2-cyclopropyl-3-oxo-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazine-8-carboxylate (compound 82-C).

(599) Example 106A (faster eluting on ChiralPak AD-3 column with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (18 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.96 (d, J=3.0 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.33-7.24 (m, 2H), 7.15 (t, J=8.2 Hz, 1H), 6.25 (s, 1H), 4.14-4.01 (m, 3H), 3.97-3.88 (m, 2H), 3.80 (dd, J=2.0, 13.3 Hz, 1H), 3.75-3.69 (m, 1H), 3.36 (d, J=4.3 Hz, 1H), 3.24-3.15 (m, 2H), 3.13-3.07 (m, 1H), 3.01 (dd, J=2.9, 10.9 Hz, 1H), 2.76 (d, J=11.3 Hz, 1H), 2.36 (t, J=10.9 Hz, 1H), 2.22 (dt, J=3.1, 11.6 Hz, 1H), 1.22 (s, 6H), 1.13 (t, J=7.2 Hz, 3H). MS: calc'd 591 (MH.sup.+), measured 591 (MH.sup.+).

(600) Example 106B (slower eluting on ChiralPak AD-3 column eluting with 30% isopropanol (0.05% DEA)/CO.sub.2) was obtained as a light yellow solid (26 mg). .sup.1H NMR (400 MHz, METHANOL-d.sub.4) d ppm 7.97 (d, J=3.3 Hz, 1H), 7.76 (d, J=3.3 Hz, 1H), 7.34-7.24 (m, 2H), 7.16 (t, J=8.2 Hz, 1H), 6.24 (s, 1H), 4.13-4.01 (m, 3H), 3.96-3.84 (m, 3H), 3.66 (t, J=9.2 Hz, 1H), 3.30-3.16 (m, 3H), 3.14-3.03 (m, 1H), 2.95-2.83 (m, 2H), 2.36 (dt, J=3.4, 11.6 Hz, 1H), 2.22 (t, J=10.9 Hz, 1H), 1.21 (s, 6H), 1.13 (t, J=7.0 Hz, 3H). MS: calc'd 591 (MH.sup.+), measured 591 (MH.sup.+).

Preparation of 2-(2-hydroxy-2-methyl-propyl)-1,5,6,7,8,8a-hexahydroimidazo[1,5-a]pyrazin-3-one (Compound 106-C)

(601) ##STR00236##

(602) tert-Butyl 2-(2-hydroxy-2-methyl-propyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (compound 106-C-1) (1 mmol) was dissolved in CH.sub.2Cl.sub.2 (3 mL) followed by the slow addition of TFA (1 mL) at 0 C. After the reaction mixture was stirred at rt for 1 hour, the solvent was removed in vacuum to give the crude product 106-C, which was used directly in the next step.

Preparation of Tert-Butyl 2-(2,2-difluoroethyl)-3-oxo-5,6,8,8a-tetrahydro-1H-imidazo[1,5-a]pyrazine-7-carboxylate (Compound 106-C-1)

(603) Compound 105-C-1 was prepared in analogy to Compound 67-D by using 1-amino-2-methyl-propan-2-ol instead of 2-aminoethanol.

Example 107: HBV Inhibition Assays

(604) Cells and Culture Conditions:

(605) HepG2.2.15 and HepDE19 are stably-transfected cell lines containing the HBV genome.

(606) Both cell lines are derived from the hepatoblastoma cell line Hep G2 (American Type Culture Collection, ATCC HB-8065) by the published procedures described in references: MA Selles et al. Proc. Natl. Acad. Sci. USA 1987, 84, 1005-1009 and H Guo et al. Journal of Virology 2007, 81, 12472-12484, respectively. Both cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM)-F12 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 g/mL streptomycin, and 0.5 mg/mL of G418.

(607) While HepG2.2.15 cells constitutively support HBV replication and production of virus particles, HepDE19 cells are inducible by tetracycline. Addition of 1 g/mL tetracycline in culture medium suppresses HBV replication in HepDE19 cells, whereas switching to tetracycline-free medium resumes this process.

(608) Anti-HBV Activity In Vitro:

(609) HepG2.2.15 cells were seeded into 96-well plates (310.sup.4 cells in 100 L media per well) and incubated overnight at 37 C. The test compounds were serially half-log diluted in DMSO, then diluted 100 times in culture media. 100 L of diluted compounds were added into the plates to reach 0.5% final concentration of DMSO in every well. Five days after compound treatment, culture supernatant was collected for further analysis.

(610) For quantitative PCR detection of extracellular HBV DNA, 100 L of culture supernatant was collected and processed in MagNA Pure 96 Nucleic Acid Purification System (Roche Applied Science) for viral DNA extraction. The extracted samples were subjected to HBV DNA quantification by qPCR. The effective compound concentration at which HBV replication is inhibited by 50% (EC.sub.50) was determined as shown in Table 1.

(611) The compounds of the present invention were tested for their capacity to inhibit a HBV activity and activation as described herein. The Examples were tested in the above assays as described herein and found to have EC.sub.50<0.2 M in HepG2.2.15 assay. Particular compounds of formula (I) or other compounds of the present invention were found to have EC.sub.50<0.02 M in HepG2.2.15 assay.

(612) TABLE-US-00003 TABLE 1 Anti-HBV activity data of particular compounds in HepG2.2.15 cells Example No. EC.sub.50 (M) Example No. EC.sub.50 (M) 1 0.104 2 0.054 3 0.023 4 0.003 5 0.159 6 0.014 7 0.014 8 0.011 9 0.064 10 0.057 11 0.001 12 0.149 13 0.066 14 0.002 15 0.110 16 0.041 17 0.039 18 0.108 19 0.003 20 0.052 21 0.016 22 0.003 23 0.003 24 0.001 25 0.002 26 0.002 27 0.003 28 0.030 29 0.007 30 0.095 31 0.100 32 0.199 33 0.030 34 0.008 35 0.011 36 0.006 37 0.017 38 0.005 39 0.030 40 0.034 41 0.014 42 0.006 43 0.006 44 0.016 45 0.026 46 0.026 47 0.051 48 0.022 49 0.008 50 0.015 51 0.031 52 0.030 53 0.019 54 0.008 55 0.005 56 0.026 57 0.032 58 0.019 59 0.011 60 0.006 61 0.012 62 0.006 63 0.012 64 0.008 65 0.009 66 0.004 67 0.438 68 0.143 69 0.013 70 0.005 71 0.014 72 0.009 73 0.009 74 0.036 75 0.024 76 0.007 77 0.146 78 0.387 79 0.008 80 0.032 81 0.010 82A/82B 0.145/0.002 83B 0.009 84A/84B 0.045/0.001 85A/85B 0.259/0.006 86B 0.004 87A/87B 0.062/0.001 87B-1 0.004 88A/88B 0.677/0.005 89 0.007 90A/90B 0.096/0.300 91A/91B 0.150/0.002 92A/92B 0.078/0.0007 93 0.012 94 0.004 95 0.011 96 0.150 97A/97B 0.037/0.0009 98 0.011 99 0.014 101A/101B 0.040/0.0008 102A/102B 0.147/0.014 103A/103B 0.140/0.010 104A/104B 0.131/0.004 105A/105B 0.188/0.003 106A/106B 0.032/0.003
Cytotoxicity and Selectivity Index:

(613) HepDE19 cells were seeded into 96-well plates (510.sup.3 cells per well) and treated with compounds for EC.sub.50 determination. Five days after treatment, cell viability was measured by addition of 20 L of CCK-8 reagent. Two hours after incubation at 37 C., the absorbance at wavelengths of 450 nm and 630 nm (OD.sub.450 and OD.sub.630) was recorded by a plate reader. The concentration results in the death of 50% of the host cells (CC.sub.50) of each compound were determined.

(614) The relative effectiveness of the compound in inhibiting viral replication compared to inducing cell death was defined as the selectivity index (CC.sub.50 value/EC.sub.50 value). Based on CC.sub.50 and EC.sub.50 data, selectivity indexes were determined.

(615) Results of CC.sub.50 and the corresponding selectivity index are given in Table 2.

(616) TABLE-US-00004 TABLE 2 CC.sub.50 and selectivity index of particular compounds Example CC.sub.50 Selectivity index Example CC.sub.50 Selectivity index No. (M) (CC.sub.50/EC.sub.50) No. (M) (CC.sub.50/EC.sub.50) 4 79 23872 11 52 30635 14 23 13016 19 >100 >30303 21 >100 >6211 22 >100 >34482 24 53 37857 26 28 18620 34 81 10655 35 >100 >9259 36 >100 >18181 38 100 21276 41 >100 >7194 42 >100 >15625 44 >100 >6369 54 >100 >12500 55 >100 >20000 59 >100 >9091 60 68 11333 61 >100 >8333 62 >100 >16667 63 91 7583 64 67 8375 65 >100 >11111 66 60 15000 70 63 12600 71 >100 >7143 73 70 7778 76 >100 >14286 79 52 6500 81 85 8500 82B >100 >50000 83B >100 >11111 84B >100 >100000 85B >100 >16667 86B >100 >25000 87B >100 >100000 88B >100 >20000 89 75 10714 91B >100 >50000 92B 66 94285 93 86 7167 94 48 12000 97B >100 >100000 98 73 6636 99 >100 >7142 101B >100 >100000 102B >100 >7142 104B 27 >6750 105B >100 >33333 106B 65 21666

Example 108: Human Microsomal Metabolic Stability

(617) Human Microsomes (BD Gentest) were preincubated with test compound for 10 minutes at 37 C. in 100 mM phosphate buffer, pH 7.4. The reactions were initiated by adding NADPH or NADPH regenerating system to give a final incubation volume of 400 L. For NADPH system, the final incubations contained 1 M test compound, 0.5 mg/mL liver microsomal protein, 1 mM NADPH in 100 mM phosphate buffer, pH 7.4. For the NADPH regenerating system, the final incubations contained 1 M test compound, 0.5 mg/mL liver microsomal protein, 3 mM glucose 6-phosphate, 1 mM NADP, 3 mM MgCl.sub.2 and 0.05 mg/mL glucose 6-phosphate dehydrogenase in 100 mM phosphate buffer, pH 7.4. After incubation times of 0, 3, 6, 9, 15 and 30 minutes at 37 C., 1 sample was obtained from each time point and 50 L of each sample was removed and transferred to 150 L of methanol solution which was maintained at 4 C. and contained 2 M tolbutamide as internal standard. Following precipitation and centrifugation, the amount of compound remaining in the samples were determined by LC-MS/MS. Controls of no NADPH or no NADPH regenerating system at zero and 30 minutes were also prepared and analyzed.

(618) Results of metabolic stability study in human microsome are given in Table 3.

(619) TABLE-US-00005 TABLE 3 Metabolic stability in human microsome Human Microsomal Human Microsomal Example Clearance Example Clearance number (mL/min/kg) number (mL/min/kg) 19 0.4 20 0.1 21 2.7 25 0.4 28 4.4 29 0.3 30 1.7 31 0.3 32 1.6 33 2.5 34 1.8 35 0.3 36 4.4 37 2.5 38 2.0 39 0.4 41 2.0 42 2.0 43 3.1 44 1.7 45 0 46 0 47 0 48 3.8 49 0 50 3.6 51 2.0 52 0.0 53 5.1 54 4.6 55 0.0 56 0.0 59 2.3 60 0.0 61 0.4 62 1.7 63 5.3 64 5.6 65 6.2 66 7.1 67 4.7 68 4.5 72 5.2 73 8.1 74 1.0 75 0.0 76 0.8 78 6.7 79 3.6 80 8.3 81 0.1 82A/82B 1.7/2.7 83A/83B 0.0/2.9 84A/84B 0.0/3.6 85A/85B 4.9/0 86B 4.7 87B 5.0 88B 4.5 90A/90B 0.3/0 91B 5.8 99 6.1 101B 6.1 102B 6.2

Example 109: Lysa Solubility

(620) Samples were prepared in duplicate from 10 mM DMSO stock solutions. After evaporation of DMSO with a centrifugal vacuum evaporator, the residue was solved in 0.05 M phosphate buffer (pH 6.5), stirred for one hour and shook for two hours. After one night, the solution was filtered using a microtiter filter plate. Then the filtrate and its 1/10 dilution were analyzed by direct UV measurement or by HPLC-UV. In addition a four-point calibration curve was prepared from the 10 mM stock solutions and used for the solubility determination of the compounds. The results were in g/mL. In case the percentage of sample measured in solution after evaporation divided by the calculated maximum of sample amount was over 80%, the solubility was reported as higher than this value.

(621) Results of Lysa are given in Table 4.

(622) TABLE-US-00006 TABLE 4 Solubility data of particular compounds Example No. Lysa (g/mL) Example No. Lysa (g/mL) 19 >727.0 20 >780.0 21 >786.0 28 337 30 610 31 >775.0 32 >701.0 33 >748.0 34 326 35 >754.0 36 485 37 422 38 627 39 >770.0 41 >804.0 42 >785.0 44 207 45 >690.0 46 >744 47 >767 48 >807 49 312 50 339 51 589 53 >723 54 387 55 470 56 >740 59 >685 60 436 61 >742 62 374 63 >751 64 >669 65 194 67 >794 68 >794 71 232 72 551 73 564 74 >716 75 >774 76 565 78 >793 79 180 80 >616 81 279 82A/82B >765/>760 83A/83B >740/780 84A/84B 570/770 85A/85B >660/770 86B >740 88B >745 90A/90B >730/>740 91B >705 95 307 99 >710 102B >665

Example 110: Cytochrome P450 (Cyp450) Induction Screening Assay_mRNA Induction

(623) Materials

(624) Cell Culture

(625) Human cryopreserved hepatocytes (Life Technologies, Carlsbad, USA) were thawed and cultured in collagen I coated 96-well plates with a density of 52,000 cells/well. After attachment, medium was changed and cells were pre-cultured overnight in hepatocyte maintenance medium (HMM; Lonza, Switzerland).

(626) Test compounds were dosed to the cells next morning at an indicated concentration (up to 10 M) in HMM culture media containing gentamycin and a constant 0.1% DMSO. Similarly, dilutions of the positive inducer compounds omeprazole (prototypical inducer of human CYP1A2; final concentrations: 1 and 10 M), phenobarbital (prototypical inducer of human CYP2B6; final concentrations: 100 and 1000 M) and rifampicin (prototypical inducer of human CYP3A4; final concentrations: 1 and 10 M) were prepared from 1000 fold DMSO stock solutions in HMM containing gentamycin. Medium change was then performed and cells were exposed for 24 hours to test compounds, positive inducer compounds, or vehicle (0.1% DMSO), respectively.

(627) At the end of the compound exposure period, medium was removed and cells lysed using 100 L/well MagNA Pure LC RNA isolation tissue lysis buffer (Roche Diagnostics AG, Rotkreuz, Switzerland). Plates were then sealed and frozen at 80 C. until further workup.

(628) mRNA Isolation, Processing and qRT-PCR

(629) mRNA isolation was performed using the MagNA Pure 96 system (Roche Diagnostics AG, Rotkreuz, Switzerland) and the respective cellular RNA large volume kit (Roche Diagnostics AG, Rotkreuz, Switzerland) from thawed samples diluted 1:1 with PBS. The volume of the cell lysis and an elution volume of 100 L were used. 20 L of the resulting mRNA suspension was then used for reverse transcription using 20 L of the transcript or first stand cDNA synthesis kit (Roche prime Supply, Mannheim, Germany). The resulting cDNA was diluted with 40 L of H.sub.2O before using for qRT-PCR. qRT-PCR was performed by using the forward and the reverse primer, the corresponding UPL (all from Microsynth, Balgach, Switzerland) and the Taqman Fast advanced master mix (Applied Biosystems), on an ABI 7900 machine (Applied Biosystems).

(630) Calculations

(631) qRT-PCR Ct-values for the respective P450s were put into relation to the Ct-value of RN18S1 (microsynth, Balgach, Switzerland) of the same sample. Doing so, a respective ct-value was calculated. Using the average of all ct-values for the vehicle control samples, a ct-value was calculated for each sample (ct-value(sample)=ct-value(sample)average of ct-value of all vehicle controls). The fold induction of the respective sample was calculated as 2{circumflex over ()}(ct). The individual fold induction values were then averaged per treatment condition (usually n=3 biological replicates).

(632) Relative induction values to the respective positive inducer compound condition (10 M omeprazole for CYP1A2; 1000 M Phenobarbital for CYP2B6; 10 M rifampicin for CYP3A4) were then calculated from the fold induction values as follows:
Relative induction (%)=100(TV)/(PV)

(633) T: fold induction of test compound condition

(634) P: fold induction of positive inducer compound

(635) V: fold induction of vehicle controls

(636) Results of CYP3A4 induction are given in Table 5.

(637) TABLE-US-00007 TABLE 5 Relative induction values of particular compounds to 10 M rifampicin % of Positive control Example No. (10 M Rifampicin) 11 17 19 1 21 2.4 22 0 25 0 27 0.2 30 7.3 34 0.2 36 6 38 0.2 42 0.6 43 0.2 45 20 54 0 55 0.1 59 2.2 62 0 73 0 76 1.3 82B 6 86B 11 87B 4.0 88B 3.7 91B 1.7