Traditional Chinese herbal plant composition for improving symptoms of liver cancer patients and compatibility method thereof

Abstract

Disclosed are a traditional Chinese herbal plant composition for improving symptoms of liver cancer patients and a compatibility method thereof. The herbal plant composition consists of the following raw materials by weight: 20-28 parts of yangtao actinidia root (Actinidia chinensis Planch.), 17-22 parts of bird's-foot trefoil (Lotus corniculatus L.), 12-15 parts of shorthairy antenoron (Anoectochilus roxburghii (Wall.) Lindl.), 7-10 parts of giant knotweed rhizome (Polygoni Cuspidati Rhizoma et Radix), 1-5 parts of fruit of mountain spicy tree (Piper cubeba L.f.), 5-10 parts of astragalus milkvetch root (Astragali Radix), 1-5 parts of hispid fig root (Ficus simplicissima Lour.), and 1-3 parts of liquorice root (Glycyrrhizae Radix et Rhizoma). The herbal plant composition can inhibit the growth of liver cancer cells without side effects.

Claims

1. A traditional Chinese herbal plant composition for ameliorating symptoms of liver cancer in patients, wherein the herbal plant composition consists of the following raw materials by weight: 20-28 parts of yangtao actinidia root (Actinidia chinensis Planch.), 17-22 parts of bird's-foot trefoil (Lotus corniculatus L.), 12-15 parts of shorthairy antenoron (Anoectochilus roxburghii (Wall.) Lindl.), 7-10 parts of giant knotweed rhizome (Polygoni Cuspidati Rhizoma et Radix), 1-5 parts of fruit of mountain spicy tree (Piper cubeba L.f.), 5-10 parts of astragalus milkvetch root (Astragali Radix), 1-5 parts of hispid fig root (Ficus simplicissima Lour.), and 1-3 parts of liquorice root (Glycyrrhizae Radix et Rhizoma).

2. The herbal plant composition according to claim 1, wherein the herbal plant composition consists of the following raw materials by weight: 25 parts of the yangtao actinidia root, 19 parts of the bird's-foot trefoil, 13 parts of the shorthairy antenoron, 8 parts of the giant knotweed rhizome, 3 parts of the fruit of mountain spicy tree, 7 parts of the astragalus milkvetch root, 4 parts of the hispid fig root, and 2 parts of the liquorice root.

3. A preparation method of the herbal plant composition according to claim 1, comprising the following steps: (1) weighing the crude drugs according to the parts by weight and mixing evenly, adding water for soaking, decocting, and then filtering to obtain a first filtrate, and retaining dregs; (2) adding water to the dregs of step (1) for soaking, decocting, and then filtering to obtain a second filtrate; and (3) combining the first filtrate and the second filtrate and concentrating to an extract having a relative density of 1.0 to 1.1.

4. The preparation method of the herbal plant composition according to claim 3, wherein the amount of water used in the step (1) and the step (2) is 20-30 times the total weight of the crude drugs.

5. The preparation method of the herbal plant composition according to claim 3, wherein in the step (1), the soaking time is 2-3 h, and the decocting time is 3-4 h; and in the step (2), the soaking time is 3-4 h, and the decocting time is 3-4 h.

6. The preparation method of the herbal plant composition according to claim 3, wherein excipients are added to the herbal plant composition to prepare a dosage form.

7. The preparation method of the herbal plant composition according to claim 6, wherein the dosage form is an oral dosage form.

8. The preparation method of the herbal plant composition according to claim 7, wherein the oral dosage form is oral liquid, tablet, powder, granule or capsule.

Description

DETAILED DESCRIPTION OF THE EMBODIMENTS

(1) The technical solutions of the present application will be further described below in conjunction with specific embodiments. However, it will be understood by those skilled in the art that the following embodiments are merely for illustration of the present application and should not be considered as limitation to the present application. Specific conditions not specified in the embodiments followed conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, were commercially available conventional products.

EMBODIMENTS

Embodiment 1

(2) The present embodiment provides a traditional Chinese herbal plant composition for improving symptoms of liver cancer patients, where the herbal plant composition consisted of the following raw materials by weight: 25 parts of root of Actinidia chinensis, 19 parts of bird's-foot trefoil, 13 parts of shorthairy antenoron, 8 parts of giant knotweed rhizome, 3 parts of fruit of mountain spicy tree, 7 parts of astragalus milkvetch root, 4 parts of hispid fig root, and 2 parts of liquorice root.

(3) The present embodiment also provides a compatibility method of the herbal plant composition, and a specific preparation process is as follows: (1) the crude drugs were weighed according to the above-mentioned parts by weight and mixed evenly, and then placed into a high-necked round bottom flask; distilled water was added to the high-necked round bottom flask at a ratio of the amount of distilled water added to the total weight of the crude drugs being 25:1 to soak the crude drugs for 2.5 h, and then the crude drugs were decocted for 3.2 h; and a first decoction liquid resulting from the decoction was filtered with 2 layers of gauze, 4 layers of gauze, 8 layers of gauze and 16 layers of gauze successively, resulting in a first filtrate; (2) dregs obtained by filtering the first decoction liquid in step (1) were placed into a high-necked round bottom flask, the same amount of distilled water as that in the step (1) was added to the high-necked round bottom flask to soak for 3.5 h, then the dregs were decocted for 3.5 h; and a second decoction liquid resulting from the decoction was filtered with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze and 20 layers of gauze successively, resulting in a second filtrate; and (3) the first filtrate and the second filtrate were combined and concentrated to an extract having a relative density of 1.03.

Embodiment 2

(4) The present embodiment provides a traditional Chinese herbal plant composition for improving symptoms of liver cancer patients, where the herbal plant composition consisted of the following raw materials by weight: 20 parts of root of Actinidia chinensis, 17 parts of bird's-foot trefoil, 12 parts of shorthairy antenoron, 7 parts of giant knotweed rhizome, 1 part of fruit of mountain spicy tree, 5 parts of astragalus milkvetch root, 1 part of hispid fig root, and 1 part of liquorice root.

(5) The present embodiment also provides a compatibility method of the herbal plant composition, and a specific preparation process is as follows: (1) the crude drugs were weighed according to the above-mentioned parts by weight and mixed evenly, and then placed into a high-necked round bottom flask; distilled water was added to the high-necked round bottom flask at a ratio of the amount of distilled water added to the total weight of the crude drugs being 20:1 to soak the crude drugs for 3 h, and then the crude drugs were decocted for 4 h; and a first decoction liquid resulting from the decoction was filtered with 2 layers of gauze, 4 layers of gauze, 8 layers of gauze and 16 layers of gauze successively, resulting in a first filtrate; (2) dregs obtained by filtering the first decoction liquid in step (1) were placed into a high-necked round bottom flask, the same amount of distilled water as that in the step (1) was added to the high-necked round bottom flask to soak for 3 h, then the dregs were decocted for 4 h; and a second decoction liquid resulting from the decoction was filtered with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze and 20 layers of gauze successively, resulting in a second filtrate; and (3) the first filtrate and the second filtrate were combined and concentrated to an extract having a relative density of 1.1.

Embodiment 3

(6) The present embodiment provides a traditional Chinese herbal plant composition for improving symptoms of liver cancer patients, where the herbal plant composition consisted of the following raw materials by weight: 28 parts of root of Actinidia chinensis, 22 parts of bird's-foot trefoil, 15 parts of shorthairy antenoron, 10 parts of giant knotweed rhizome, 5 parts of fruit of mountain spicy tree, 10 parts of astragalus milkvetch root, 5 parts of hispid fig root, and 3 parts of liquorice root.

(7) The present embodiment also provides a compatibility method of the herbal plant composition, and a specific preparation process is as follows: (1) the crude drugs were weighed according to the above-mentioned parts by weight and mixed evenly, and then placed into a high-necked round bottom flask; distilled water was added to the high-necked round bottom flask at a ratio of the amount of distilled water added to the total weight of the crude drugs being 30:1 to soak the crude drugs for 2 h, and then the crude drugs were decocted for 3 h; and a first decoction liquid resulting from the decoction was filtered with 2 layers of gauze, 4 layers of gauze, 8 layers of gauze and 16 layers of gauze successively, resulting in a first filtrate; (2) dregs obtained by filtering the first decoction liquid in step (1) were placed into a high-necked round bottom flask, the same amount of distilled water as that in the step (1) was added to the high-necked round bottom flask to soak for 4 h, then the dregs were decocted for 3 h; and a second decoction liquid resulting from the decoction was filtered with 3 layers of gauze, 6 layers of gauze, 12 layers of gauze and 20 layers of gauze successively, resulting in a second filtrate; and (3) the first filtrate and the second filtrate were combined and concentrated to an extract having a relative density of 1.0.

COMPARATIVE EXAMPLES

Comparative Example 1

(8) The comparative example provides a herbal plant composition, where the herbal plant composition consisted of the following raw materials by weight: 25 parts of root of Actinidia chinensis, 13 parts of shorthairy antenoron, 8 parts of giant knotweed rhizome, 3 parts of fruit of mountain spicy tree, 7 parts of astragalus milkvetch root, 4 parts of hispid fig root and 2 parts of liquorice root, with the rest the same as in Embodiment 1.

Comparative Example 2

(9) The comparative example provides a herbal plant composition, where the herbal plant composition consisted of the following raw materials by weight: 25 parts of root of Actinidia chinensis, 19 parts of bird's-foot trefoil, 21 parts of giant knotweed rhizome, 3 parts of fruit of mountain spicy tree, 7 parts of astragalus milkvetch root, 4 parts of hispid fig root and 2 parts of liquorice root, with the rest the same as in Embodiment 1.

Comparative Example 3

(10) The comparative example provides a herbal plant composition, where the herbal plant composition consisted of the following raw materials by weight: 25 parts of root of Actinidia chinensis, 19 parts of bird's-foot trefoil, 21 parts of shorthairy antenoron, 3 parts of fruit of mountain spicy tree, 7 parts of astragalus milkvetch root, 4 parts of hispid fig root and 2 parts of liquorice root, with the rest the same as in Embodiment 1.

EFFECT EXAMPLES

Effect Example 1

(11) The effect example explored the inhibitory effects of the herbal plant compositions of Embodiments 1-3 and Comparative Examples 1-3 on Hep G2 tumor cells, and a specific process is as follows.

(12) The extracts of Embodiments 1-3 and Comparative Examples 1-3 were diluted to concentrations of 10 mg/mL, 40 mg/mL and 80 mg/mL using RPMI-1640 complete medium, and filtered and sterilized using 0.25 m sterile syringe filters to obtain drug-containing media for later use. In addition to the drug-containing medium group, a negative control group without the drugs and a pure culture medium group were set, and 5 replicate wells were set for all groups.

(13) The Hep G2 cells were resuspended in the RPMI-1640 complete medium, and a cell density was adjusted to 510.sup.4 cells/mL, resulting in a Hep G2 cell suspension. The Hep G2 cell suspension was added to a 96-well plate, with 100 L per well, and cultured in a 37 C. and 5% CO.sub.2 cell incubator for 24 h. After the cells adhered to the wall, a resulting supernatant was discarded, 100 L of each group of drug-containing medium was added to each well to incubate the cells in the 37 C. and 5% CO.sub.2 cell incubator for 72 h. After the incubation, 10 L of CCK-8 solution was added to each well to continuously culture the cells in the incubator for 4 h. Then, an OD value of each group of cells at 450 nm was detected using a microplate reader, the inhibition rate was calculated as (OD value of negative control group-OD value of administration group)/(OD value of negative control group-OD value of pure medium group)100%, and the OD value of each group was an average of the 5 replicate wells. The results are shown in Table 1.

(14) TABLE-US-00001 TABLE 1 Group/inhibition rate 10 mg/mL 40 mg/mL 80 mg/mL Embodiment 1 3.05% 27.60% 96.24% Embodiment 2 2.94% 27.15% 95.83% Embodiment 3 2.86% 26.33% 95.17% Comparative Example 1 1.81% 17.48% 83.69% Comparative Example 2 2.53% 24.09% 92.86% Comparative Example 3 2.12% 22.85% 91.15%

(15) It can be seen from Table 1 that when the drug concentration was 10-80 mg/mL, the herbal plant compositions of Embodiments 1-3 can inhibit the growth of liver cancer cells to varying degrees after 72 h of administration, and the drug concentration of 80 mg/mL resulted in the best inhibitory effect.

(16) Compared with Embodiments 1-3, the inhibition rate of Hep G2 cells in Comparative Example 1 group was significantly reduced, and the inhibition rate of Hep G2 cells in Comparative Example 2-3 group was slightly reduced. It can be seen therefrom that the herbal plant composition formula of the present application can inhibit the growth of liver cancer cells.

Effect Example 2

(17) The effect example explored the anti-tumor effect of the herbal plant compositions of Embodiment 1 and Comparative Examples 1 to 3 on tumor mice, and a specific process is as follows.

(18) I. Test Materials

(19) Totally 90 male BALB/c nude mice aged 5 weeks and with body weight of 19-22 g were used.

(20) II. Construction of Tumor Cell Model

(21) A total of 80 BALB/c nude mice were randomly selected and injected subcutaneously with Hep G2 cell line (510.sup.6 cells) in the right armpits. After 10 d, a mass appeared in the right armpits of the nude mice, indicating that the tumor cell model was successfully established.

(22) III. Grouping and Dosing

(23) Dosing Criteria: the conventional gavage dose of mice was 103.5 g/kg0.002650=10.53 g/kg. Generally, it is also necessary to increase the dose of the original medicinal material of the herbal plant composition by 1 time and decrease the dose of the original medicinal material of the herbal plant composition by 1 time for gavage.

(24) Model group: 10 mice, administered equal volume of physiological saline by gavage.

(25) Low-dose Embodiment 1 Herbal Plant Composition Group: 10 mice, administered 5.27 g/kg Embodiment 1 herbal plant composition by gavage.

(26) Medium-dose Embodiment 1 Herbal Plant Composition Group: 10 mice, administered 10.53 g/kg Embodiment 1 herbal plant composition by gavage.

(27) High-dose Embodiment 1 Herbal Plant Composition Group: 10 mice, administered 21.06 g/kg Embodiment 1 herbal plant composition by gavage.

(28) High-dose Comparative Example 1 Herbal Plant Composition Group: 10 mice, administered 21.06 g/kg Comparative Example 1 herbal plant composition by gavage.

(29) High-dose Comparative Example 2 Herbal Plant Composition Group: 10 mice, administered 21.06 g/kg Comparative Example 2 herbal plant composition by gavage.

(30) High-dose Comparative Example 3 Herbal Plant Composition Group: 10 mice, administered 21.06 g/kg Comparative Example 3 herbal plant composition by gavage.

(31) Western medicine control group: 10 mice, administered 0.03 g/kg 5-fluorouracil by gavage.

(32) Blank control group: 10 mice, administered equal volume of physiological saline by gavage.

(33) IV. Detection of Tumor Inhibition Rate of Each Group

(34) After 28 d of continuous administration, the mice in each group were weighed and killed by cervical dissection. Tumors and organs (lung, liver, heart and kidney) of the mice in each group were removed under sterile conditions, and washed with physiological saline. After washing, the tumors and the organs were dried with filter paper, weighed to obtain mass and recorded, and the tumor inhibition rate and organ index were calculated. The tumor mass of the mice was the average value of the tumor mass of each group of mice. Tumor inhibition rate=(tumor mass of mice in the model group-tumor mass of mice in the test group)/tumor mass of mice in the model group100%. The tumor mass and tumor inhibition rate of mice in each group are shown in Table 2. Organ index (mg/g)=organ mass (mg)/body mass (g) of mice. The organ index is the average value of the organ index of each group of mice, and the results are shown in Table 3.

(35) TABLE-US-00002 TABLE 2 Tumor Inhibition Grouping Dosage mass (g) rate (%) Model group / 0.52 / Western medicine 0.03 g/kg 0.27 48.07 control group Low-dose 5.27 g/kg 0.44 15.38 Embodiment 1 Medium-dose 10.53 g/kg 0.30 42.31 Embodiment 1 High-dose 21.06 g/kg 0.28 46.15 Embodiment 1 High-dose 21.06 g/kg 0.42 19.23 Comparative Example 1 High-dose 21.06 g/kg 0.35 32.69 Comparative Example 2 High-dose 21.06 g/kg 0.37 28.85 Comparative Example 3

(36) TABLE-US-00003 TABLE 3 Liver Renal Lung Cardiac index index index Index Grouping Dosage (mg/g) (mg/g) (mg/g) (mg/g) Model group / 42.31 17.25 5.62 4.87 Western medicine 0.03 g/kg 42.78 17.69 5.96 5.13 control group Low-dose 5.27 g/kg 42.64 17.52 5.87 5.05 Embodiment 1 Medium-dose 10.53 g/kg 42.91 17.60 6.24 5.17 Embodiment 1 High-dose 21.06 g/kg 42.38 17.41 5.69 4.92 Embodiment 1 High-dose 21.06 g/kg 42.80 17.72 5.83 5.10 Comparative Example 1 High-dose 21.06 g/kg 42.47 17.50 5.72 4.95 Comparative Example 2 High-dose 21.06 g/kg 42.51 17.48 5.75 4.99 Comparative Example 3

(37) As can be seen from Table 2, the medium-dose herbal plant composition, the high-dose herbal plant composition and the western medicine group had relatively high tumor inhibition rates in mice, indicating that the medium-dose and high-dose herbal plant compositions can be applied to the treatment of liver cancer.

(38) As can be seen from Table 3, compared to the model group, the liver index, renal index, lung index and cardiac index of the tumor mice in the model group, the low-dose herbal plant composition group, the medium-dose herbal plant composition group, the high-dose herbal plant composition group and the western medicine control group showed no significant change. The above results indicate that the herbal plant composition of the present application has no effect on the major organ index of tumor mice. That is, the herbal plant composition of the present application can effectively treat liver cancer without side effects.

(39) Finally, it is to be understood that the above embodiments are merely illustrative of the technical solutions of the present application, and are not restrictive to them. The basic principles and main features of the present application have been described above with specific embodiments, and some modifications or substitutions may be made on the basis of the present application, but these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the claimed scope of the present application.