DIAGNOSIS OF RESPIRATORY DISEASES USING ANALYSIS OF EXHALED BREATH AND AEROSOLS

Abstract

Disclosed are methods and devices for analyzing non-volatile organics in exhaled breath and other aerosols using various diagnostic tools that enable rapid, low cost point of care assays for several diseases including respiratory tract diseases such as COVID-19. The disclosed methods and systems selectively capture non-volatile organics in exhaled breath and other aerosols in a packed bed column. The non-volatile organics are eluted and samples are analysis using diagnostic devices including MALDI-TOFMS. The disclosed systems and methods provide for a diagnostic test result in less than about 20 minutes and provides for autonomous operation with minimal human intervention.

Claims

1. A breath sample collection system for diagnosis of at least one respiratory disease using exhaled breath, the system comprising: a breath collection element configured to receive an individual's face for collecting aerosolized bacteria and virus particles in exhaled breath wherein the breath collection element forms a tight-fit with the individuals face; a sample capture element comprising a packed bed column to selectively capture the aerosolized bacteria and virus particles wherein the sample capture element is removably connected to a port disposed in the breath collection element proximate to the individual's chin when the breath collection element is positioned on the individual's face without any interconnecting tubing; and, a pump in fluid communication with the sample capture element and configured to draw exhaled breath into the sample capture element wherein the particle capture efficiency of the breath sample collection system is greater than 99%.

2-6. (canceled)

7. The system of claim 1 wherein the packed bed column comprises solid particles comprising at least one of resins, cellulose, silica, agarose, and hydrated Fe.sub.3O.sub.4 nanoparticles.

8. The system of claim 1 wherein the packed bed column comprises resin beads having C18 functional groups on the surface.

9. The system of claim 8 wherein the resin beads have a nominal diameter of between about 12 μm and about 20 μm.

10-14. (canceled)

15. The system of claim 1 wherein the nominal flow rate pulled through the packed bed column by the pump is between about 200 ml/min and about 600 ml/min.

16. The system of claim 1 wherein the breath collection element comprises at least one of a CPR rescue mask, a CPAP mask, and a ventilator mask.

17. The system of claim 1 further comprising a trap disposed between the sample capture element and the pump and configured to trap exhaled breath condensate (EBC) comprising at least one of water vapor, volatile organic components and non-volatile organic components that pass through the packed bed wherein the trap is cooled below ambient temperature.

18. (canceled)

19. The system of claim 7 wherein the solid particles comprise functional groups immobilized on the surface of the particles wherein the functional groups comprise at least one of C18 (octadecyl), octyl, ethyl, cyclohexyl, phenyl, cyanopropyl, aminopropyl, 2,3-dihydroxypropoxypropyl, trimethyl-aminopropyl, carboxypropyl, benzenesulfonic acid, propylsulfonic acid, an ion exchange phase, a polymer phase, antibodies, glycans, lipids, DNA and RNA.

20-22. (canceled)

23. The system of claim 1 wherein the capture element is cooled to a temperature at or below ambient temperature.

24. (canceled)

25. A sample capture element for diagnosis of a respiratory disease using exhaled breath comprising: a packed bed column to selectively capture aerosolized bacteria and virus particles in exhaled breath drawn through the packed bed column using a pump wherein the packed bed column comprises: solid particles having a nominal diameter of between about 12 μm and about 20 μm comprising at least one of resins, cellulose, silica, agarose, and hydrated Fe.sub.3O.sub.4 nanoparticles; and, functional groups immobilized on the surface of the particles wherein the functional groups comprise at least one of C18 (octadecyl), octyl, ethyl, cyclohexyl, phenyl, cyanopropyl, aminopropyl, 2,3-dihydroxypropoxypropyl, trimethyl-aminopropyl, carboxypropyl, benzenesulfonic acid, propylsulfonic acid, ion exchange phases, polymer phases, antibodies, glycans, lipids, DNA and RNA, wherein the particle capture efficiency of the sample capture element is greater than 99% as the exhaled breath is drawn through the packed bed column at a flow rate of between about 200 ml/min and about 600 ml/min.

26. (canceled)

27. The element of claim 25 wherein the solid particles are packed between two porous polymeric frit discs.

28. A system for diagnosis of a respiratory disease using exhaled breath, the system comprising: the breath sample collection system of claim 1; a sample extraction system to extract captured virus and bacteria particles from the packed bed column; and, a sample analysis system comprising: a sample processing system for treating and concentrating the collected sample on a sample plate; and, a diagnostic device for analyzing the sample.

29. The system of claim 28 wherein the diagnostic device comprises at least one of PCR, ELISA, rt-PCR, mass spectrometer (MS), MALDI-MS, ESI-MS, and MALDI-TOFMS.

30. (canceled)

31. The system of claim 28 wherein the extraction system comprises means to flush the pack bed column with a solvent and remove the solvent comprising aerosolized bacteria and virus particles from the packed bed.

32. The system of claim 31 wherein the solvent comprises at least one of acetonitrile, methanol, acid, isopropanol, the remaining being water.

33-45. (canceled)

46. A breath sample collection system for diagnosis of at least one respiratory disease using exhaled breath, the system comprising: a mask configured to receive an individual's face for collecting aerosolized bacteria and virus particles in exhaled breath wherein the mask forms a tight-fit with the individuals face and wherein the mask comprises a stem and port disposed below the stem proximate to the individual's chin when the mask is positioned on the individual's face; a HEPA filter removably and fluidly connected to the stem of the mask; a sample capture element comprising a packed bed column to selectively capture the aerosolized bacteria and virus particles wherein the sample capture element is removably connected to the port; and, a pump in fluid communication with the sample capture element and configured to draw exhaled breath into the sample capture element wherein the particle capture efficiency of the sample capture element is greater than 99% as the exhaled breath is pulled through the packed bed column by the pump at a flow rate of between about 200 ml/min and about 600 ml/min.

47. (canceled)

48. The system of claim 46 wherein the packed bed column comprises solid particles comprising at least one of resins, cellulose, silica, agarose, and hydrated Fe.sub.3O.sub.4 nanoparticles.

49. The system of claim 46 wherein the packed bed column comprises resin beads having C18 functional groups on the surface.

50. The system of claim 49 wherein the resin beads have a nominal diameter of between about 12 μm and about 20 μm.

51-65. (canceled)

66. The system of claim 28 wherein the sample processing system comprises: means for hot acid digestion of the bacteria and virus particles extracted from the sample extraction system to generate a peptide sample characteristic of the particles; mixing the peptide sample with a MALDI matrix; and, applying the mixed sample and MALDI matrix to a sample plate.

67-69. (canceled)

Description

DRAWINGS

[0026] The foregoing aspects and many of the attendant advantages of this disclosure will become more readily appreciated as the same becomes better understood by reference to the following detailed description, when taken in conjunction with the accompanying drawings, wherein:

[0027] FIG. 1. Schematic diagram of an exemplary exhaled breath sample collection system comprising a packed bed column.

[0028] FIG. 2. Schematic diagram of an exemplary diagnostic system for respiratory diseases comprising a sample collection system.

[0029] FIG. 3. Schematic diagram of an exemplary diagnostic method using a system that comprises a packed bed column.

[0030] FIG. 4. Particle capture efficiency measurements using an exemplary packed bed column.

[0031] FIGS. 5A-E show the results of nonvolatile organic molecules collected using an exemplary packed bed column and analyzed using mass spectrometry.

[0032] FIGS. 6A and 6B depict silver staining images with SDS-PAGE electrophoresis of proteins in exhaled breath samples, and spectra collected using total ion chromatography (TIC) in LC-MS analysis of exhaled breath samples, respectively.

[0033] FIG. 7. Schematic diagram of an exemplary exhaled breath sample collection system comprising a packed bed column.

[0034] FIGS. 8A and 8B show the results of aerosolized bacteria and virus captured using an exemplary packed bed column and analyzed using MALDI TOF-MS.

[0035] All reference numerals, designators and callouts in the figures are hereby incorporated by this reference as if fully set forth herein. The failure to number an element in a figure is not intended to waive any rights. Unnumbered references may also be identified by alpha characters in the figures and appendices.

[0036] The following detailed description includes references to the accompanying drawings, which form a part of the detailed description. The drawings show, by way of illustration, specific embodiments in which the disclosed systems and methods may be practiced. These embodiments, which are to be understood as “examples” or “options,” are described in enough detail to enable those skilled in the art to practice the present invention. The embodiments may be combined, other embodiments may be utilized, or structural or logical changes may be made, without departing from the scope of the invention. The following detailed description is, therefore, not to be taken in a limiting sense and the scope of the invention is defined by the appended claims and their legal equivalents.

[0037] In this disclosure, aerosol generally means a suspension of particles dispersed in air or gas. “Autonomous” diagnostic systems and methods mean generating a diagnostic test result “with no or minimal intervention by a medical professional.” The U.S. FDA classifies medical devices based on the risks associated with the device and by evaluating the amount of regulation that provides a reasonable assurance of the device's safety and effectiveness. Devices are classified into one of three regulatory classes: class I, class II, or class III. Class I includes devices with the lowest risk and Class III includes those with the greatest risk. All classes of devices as subject to General Controls. General Controls are the baseline requirements of the Food, Drug and Cosmetic (FD&C) Act that apply to all medical devices. In vitro diagnostic products are those reagents, instruments, and systems intended for use in diagnosis of disease or other conditions, including a determination of the state of health, in order to cure, mitigate, treat, or prevent disease or its sequelae. Such products are intended for use in the collection, preparation, and examination of specimens taken from the human body. The exemplary devices disclosed herein can operate and produce a high-confidence result autonomously, and consequently, has the potential to be regulated as a Class I device. In some regions of the world with high burdens of TB infection, access to medically trained personnel is very limited. An autonomous diagnostic system is preferred to one that is not autonomous.

[0038] The terms “a” or “an” are used to include one or more than one, and the term “or” is used to refer to a nonexclusive “or” unless otherwise indicated. In addition, it is to be understood that the phraseology or terminology employed herein, and not otherwise defined, is for the purpose of description only and not of limitation. Unless otherwise specified in this disclosure, for construing the scope of the term “about,” the error bounds associated with the values (dimensions, operating conditions etc.) disclosed is ±10% of the values indicated in this disclosure. The error bounds associated with the values disclosed as percentages is ±1% of the percentages indicated. The word “substantially” used before a specific word includes the meanings “considerable in extent to that which is specified,” and “largely but not wholly that which is specified.”

DETAILED DISCLOSURE

[0039] Breath aerosol particles contain a variety of nonvolatile organic biomolecules such as metabolites, lipids, and proteins. Further, the nonvolatile molecules have a wide particle size distribution ranging from a sub-micron size to about 10 microns in size. Breath collection and disease diagnostic systems and methods that can efficiently capture different types of nonvolatile molecules of different particle sizes from exhaled breath are required. Particular aspects of the invention are described below in considerable detail for the purpose for illustrating the compositions, and principles, and operations of the disclosed methods and systems. However, various modifications may be made, and the scope of the invention is not limited to the exemplary aspects described.

[0040] An exemplary diagnosis system 2000 (FIG. 2) based on exhaled breath analysis (“EBA”) may comprise a breath sample collection system 1000 disposed in fluid communication with a sample extraction system 2002 and an analysis system 2003.

[0041] An exemplary exhaled breath sample collection system 1000 (FIG. 1) may comprise a sample capture element 1001 comprising a packed bed column to selectively capture breath aerosol comprising nonvolatile organisms including, but not limited to bacteria and viruses, and molecules including small molecules, lipids, and proteins on very high efficiency adsorbent materials. A trap 1003 is in fluid communication with column 1001 using tubing 1002. Trap 1003 may be made of glass or plastic material. Trap 1003 may be cooled to below ambient temperature using an ice bath or other suitable means. Trap 1003 may be used to collect water vapor, other volatile (check) and nonvolatile molecules that may pass through the collection column as exhaled breath condensate (EBC). During breath analysis of a patient breathing using a ventilator, sample capture element 1001 may be removably connected to the capnography port on the respirator tubing of a ventilator, placing it very near the outlet or at the outlet from the patient's lungs. During breath analysis of a normally breathing person, element 1001 may be removably connected to a mouthpiece (not shown) into which the patient is instructed to breathe or otherwise execute a breath maneuver previously disclosed herein. For example, capture element 1001 may be removably connected downstream (at the outlet) of a breath collection element 1007 (FIG. 1) such as a first aid CPR rescue mask (e.g., as supplied by Dixie USA EMS Supply Co., Model Number EVR-CPR01) worn by the patient during breath analysis. A flow splitter 1008 may be disposed between breath collection element 1007 and capture element 1001 to divide the flow of exhaled breath such that a first portion of exhaled breath is directed to capture element 1001 and a second portion towards a HEPA filter 1009. Flow splitter 1008 may be integrated into collection element 1007. Further, a large particle trap 1012 may be disposed upstream of capture element 1001 to remove large particles of breath condensate (greater than about 10 μm) from the exhaled breath stream prior to entering capture element 1001. Pump 1006 may be used to pull exhaled breath into the packed bed column in capture element 1001. An exemplary pump 1006 is a portable diaphragm pump (e.g., Parker Hannifin Corp., Part No.: D737-23-01). The flow rate out of pump 1006 may be adjusted using needle valve 1005 to achieve a desired flow rate. Check valve (one-way flow valve) 1011 may be disposed between pump 1006 and capture element 1001 and is configured to be in an open position only when pump 1006 is pulling exhaled breath through the packed bed column. When there is no flow, valve 1011 is disposed in a closed position. A nominal flow rate of between about 200 ml/min and 600 ml/min may be used. In addition, several capture elements 1001 may be used in parallel to increase the flow rate up to 12 L/min. Further, when one or more capture elements are in collection mode, one or more may be in eluting mode, and the some may be in standby mode. To determine if exhaled breath sample volume was adequate, a CO.sub.2 sensor and particle counter (not shown) may be disposed between breath collection element 1007 and sample capture element 1001. CO.sub.2 monitoring and particle count allows for an approximation of the proportion of exhaled air volume. A HEPA filter may also be disposed downstream of trap 1003. Capture element 1001 may be cooled using a cooling jacket or other means to reduce the temperature to below ambient temperature to increase the collection efficiency of non-volatile organics particles. The breath sample collection system may further comprise a humidifier 1010 disposed upstream of the inlet to the capture element to humidify exhaled breath and increase the humidity in the packed bed column.

[0042] Breath collection element 1007 may comprise a tight-fitting mask configured to receive an individual's face and may be removably attached using straps and the like to the face/head of a patient/individual. The individual may sit in an optional containment booth to isolate the patient's EBA from the ambient air in the testing room or area. Element 1007 may be used to collect and direct breath aerosol particles emitted though the mouth and nose of patient into capture element 1001 using pump 1006 as previously described without depositing the aerosol particles on the walls of element 1007. Element 1007 may be disposable to limit the risk of a patient becoming contaminated or infected with a pathogen emitted by a previous patient. Alternatively, element 1007 may be reusable, in which case it may be sterilized.

[0043] The exemplary packed bed column in capture element 1001 may comprise Hamilton PRP-C18 resin beads as supplied by Sigma Aldrich and other vendors. The bed may be held in place between two porous filter plates such as frit discs. For example, a polyethylene disc having an average pore size of above 35 μm may be placed upstream of the bed and a polyethylene disc having an average pore size of 10 μm (Boca Scientific, Dedham, Mass.) may be placed downstream of the bed. The 35 μm frit disc allows a faster air flow rate while the smaller 10 μm frit disc traps all the C18 resin well. In an exemplary element 1001, the packed bed may comprise about 25 mg of C18 resin beads having a nominal diameter between about 12 μm and about 20 μm. Non-volatile organic components in exhaled breath removably interact with the C18 functional groups on the beads and are trapped. Water, volatiles and other hydrophilic molecules pass through the bed and may be trapped in glass trap 1003.

[0044] Besides C18 functional groups, other functional groups that show affinity to nonvolatile molecules may be used as adsorbents in the column immobilized on solid phase beads such as resin beads. The solid phase beads may be made of polymers and particles such as resins, cellulose, silica, agarose, and hydrated Fe.sub.3O.sub.4 nanoparticles. Adsorbent materials may comprise other functional groups that include, but are not limited to, octadecyl, octyl, ethyl, cyclohexyl, phenyl, cyanopropyl, aminopropyl, 2,3-dihydroxypropoxypropyl, trimethyl-aminopropyl, carboxypropyl, benzenesulfonic acid, and propylsulfonic acid disposed on solid phase beads. Functional groups may also comprise at least one of ion exchange phases, polymer phases, antibodies, glycans, lipids, DNA and RNA.

[0045] Exemplary diagnosis system 2000 (FIG. 2) may comprise a breath sample collection system 2001 disposed in fluid communication with a sample extraction system 2002 and an analysis system 2003. Sample collection system 2001 may comprise exemplary sample collection system 1000 (FIG. 1) as previously described. Sample extraction system 2002 may be used to extract the trapped non-volatile organics from the packed bed column in system 1000 and may be disposed in-line or off-line in system 2000. When system 2002 is disposed off-line, at the conclusion of exhaled breath sample collection, capture element 1001 may be removed from system 1000 and eluted with an organic solvent in extraction system 2002 to remove non-volatile organics from the packed bed column. Exemplary organic solvents include, but are not limited to, about 50-70% acetonitrile in water to extract trapped non-volatile organics (strongly polar non-volatile organic molecules, proteins and the like) from the packed bed column. The extraction may be repeated using the same or another solvent, that includes, but is not limited to 50-70% isopropanol in water to extract less polar lipid molecules from the packed bed. Other organic solvents include between about 50% and about 70% methanol in water, and about 50% methanol in about 50% chloroform. When system 2002 is disposed in-line, at least one of a CO.sub.2 sensor and particle counter may be disposed upstream of extraction system 2002. System 2002 may comprise a solvent vessel, a pump to transfer the solvent from the solvent to packed bed column and a vessel to collect the solvent comprising the non-volatile biomarkers into another vessel or cup. Alternately, system 2002 may comprise an injector to inject solvent into the packed bed column and collect the extract liquid comprising non-volatile organics and biomarkers in a suitable cup or vessel, or other laboratory tubes having a small volume. The captured sample in solvent may be further processed and analyzed in analysis system 2003.

[0046] Analysis system 2003 may comprise sample processing system 2004 and at least one diagnostic device 2005. Sample processing system 2004 may comprise elements necessary to perform one or more of the following steps:

[0047] (a) Placing the sample in at least one of a cup, a vial and a sample plate. For example, the Series 110A Spot Sampler (Aerosol Devices) uses 32 well plates with circular well shape (75 μL well volume) or teardrop well shape (120 μL well volume) which are heated to evaporate the solvent and excess fluid/liquid in the sample to concentrate the sample;

[0048] (b) Placing the sample in a cup and exposed to a source of vacuum or freeze-drying device to cause the solvent to evaporate to concentrate the sample; and,

[0049] (c) hot digestion of proteins and virus particles

[0050] The samples may be centrifuged to remove chemical contamination particles. Many diagnostic devices may be adapted for use in analysis system 2003 that include, but are not limited to devices that perform genomics-based assays (such as PCR, rt-PCR and whole genome sequencing), biomarker recognition assays (such as ELISA), and spectral analysis such as mass spectrometry (MS). Of these diagnostic devices, MS is preferable on account of its speed of analysis. The MS techniques that are preferable for biomarker identification are electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) time of flight MS (TOFMS). ESI may be coupled to high resolution mass spectrometers. MALDI-TOFMS devices may be compact, lightweight, consume less than 100 watts of power and provide sample analysis in less than 15 minutes. MALDI-TOFMS is a preferred diagnostic device for point-of-care diagnostics suitable for ACF. The sample must be dry before it is inserted into the vacuum chamber of the MS and subjected to laser pulses from an ultraviolet laser. This interaction between the sample and the laser creates large, informative biological ion clusters that are characteristic of the biological material. When a concentrated sample is provided by sample processing system 2004 comprising only trace levels of water or trace levels organic solvents such as 50% to 70% of one of acetonitrile, methanol, and isopropanol in water, sample analysis using MS may take less than 5 minutes (including the sample preparation) because less time is needed to evaporate the water from the sample.

[0051] MALDI-TOFMS may be used to identify live/active agents that include, but are not limited to, B. anthracis spores (multiple strains), Y. pestis, F. tularensis, Venezuelan equine encephalitis virus (VEE), Western equine encephalomyelitis virus (WEE), Eastern equine encephalitis virus (EEE), botulinum neurotoxins (BoNT), staphylococcus Enterotoxin (SEA), Staphylococcal enterotoxin B (SEB), ricin, abrin, Ebola Zaire strain, aflatoxins, saxitoxin, conotoxins, Enterobacteria phage T2 (T2), HT-2 toxins (HT2), cobra toxin, biothreat simulants including B. globigii spores, B. cereus spores, B. thuringiensis Al Hakam spores, B. anthracis Sterne spores, Y. enterocolitica, E. coli, MS2 virus, T2 virus, Adenovirus and nonvolatile biochemical threats including NGAs (nonvolatile), bradykinin, oxytocin, Substance P, angiotensin, diazepam, cocaine, heroin, and fentanyl. Further, the exemplary systems and methods disclosed herein may be used to achieve accurate detection and identification of SARS-CoV-2 from human breath samples.

[0052] In “matrix assisted laser desorption ionization” (MALDI), the target particle (analyte) is coated by a matrix chemical, which preferentially absorbs light (often ultraviolet wavelengths) from a laser. In the absence of the matrix, the biological molecules would decompose by pyrolysis when exposed to a laser beam in a mass spectrometer. The matrix chemical also transfers charge to the vaporized molecules, creating ions that are then accelerated down a flight tube by the electric field. Microbiology and proteomics have become major application areas for mass spectrometry; examples include the identification of bacteria, discovering chemical structures, and deriving protein functions. MALDI-MS has also been used for lipid profiling of algae. During MALDI-MS, a liquid, usually comprised of an acid, such as trifluoroacetic acid (TFA), and a MALDI matrix chemical such as alpha-cyano-4-hydroxycinnamic acid, is dissolved in a solvent and added to the sample. Solvents include acetonitrile, water, ethanol, and acetone. TFA is normally added to suppress the influence of salt impurities on the mass spectrum of the sample. Water enables hydrophilic proteins to dissolve, and acetonitrile enables the hydrophobic proteins to dissolve. The MALDI matrix solution is spotted on to the sample on a MALDI plate to yield a uniform homogenous layer of MALDI matrix material on the sample. The solvents vaporize, leaving only the recrystallized matrix with the sample spread through the matrix crystals. The acid partially degrades the cell membrane of the sample making the proteins available for ionization and analysis in an MS. Other MALDI matrix materials include 3,5-dimethoxy-4-hydroxycinnamic acid (sinapinic acid), α-cyano-4-hydroxycinnamic acid (α-cyano or α-matrix) and 2,5-dihydroxybenzoic acid (DHB) as described in U.S. Pat. No. 8,409,870.

[0053] Further, the volatile organic compounds collected in trap 1003 (FIG. 1) may be warmed using a heater, to drive off the volatile compounds into a diagnostic device such as GC-MS, GC-IMS, volatile ion chromatography, or any other type of analysis method suitable for analyzing volatile organic compounds.

[0054] Virus (e.g. SARS-CoV-2) detection is centered on detection of viral proteins, which is a difficult challenge. An exemplary method for virus detection may comprise a glycan-based capture matrix (beads) to pull the target virus out of the background matrix (e.g., other non-virus biomolecule, contaminants). An aliquot of the sample which may contain a virus, for example, collected using sample collection system 1000, which may also comprise other background contaminants, may be applied to a bead carrying the capture probe. At least one of glycan, heparin, and carbohydrates may be used as capture materials or probes bound on resin beads or some other types of beads. An optional washing step may be used to remove any nontargeted-virus contaminants. The concentrated and purified virus may be eluted off the beads using suitable solvents into a sealed heating chamber containing an organic acid which may comprise formic acid or acetic acid and heated to 120° C. for about 10 minutes to digest the proteinaceous toxin down into specific peptide fragments. This hot acid protein digestion protocol cleaves the protein at aspartic acid residues creating a highly reproducible peptide pattern. The capture and digestion processes described may be accomplished with antibodies and enzyme, respectively. Using this exemplary sample processing for MALDI-TOFMS, sensitivity for ricin biotoxin of better than 100 ng/mL (with S/N of about 50:1) in clean buffer was achieved. At S/N (signal to noise ratio) of 3:1, limits of detection (LOD) of <10 ng/mL may be achieved. For the 1 μL samples used in the MALDI-TOFMS analytical systems, about 10 ng/mL LOD equates to a total mass of about 10 pg (10.sup.−12 g) on the probe, which is equivalent to about 20,000 viral particles. An exemplary microfluidic sample processing system to implement the method disclosed above may be configured to analyze samples collected from the air or from other sources such as nasal swabs. The glycan-based capture column and other microfluidics components may be reusable. Large fluid reservoirs containing buffer, weak acids, and alcohols may be employed to provide sufficient capacity to measure 100's of samples in one channel of the system. Multiple systems may be run in parallel to process multiple samples simultaneously. Since no fragile and expensive biomolecular reagents are required the system is cost effective.

[0055] Hot acid digestion cleaves the proteins reproducibly at aspartic acid residues creating known peptide sequences with known masses. These peptide mass distributions are characteristic of the progenitor proteins. Thus, digestion provides outstanding specificity if the proteins of interest are largely separated from background materials. Furthermore, the peptide mass distribution is directly determined by the genome, accounting for post-translational modifications. As soon as a new virus is isolated, it is rapidly sequenced. The RNA sequence of the SARS-CoV-2 virus may be used to accurately predict the protein sequences with modern bioinformatics tools (ExPASy bioinformatics portal). These proteins can then be “digested” in silico using bioinformatics tools to create a theoretical peptide map. Thus, the peptides that arise from SARS-COV-2 digestion can be predicted and compared to experimental data to generate a specific MALDI TOFMS signature of the organism. Reports suggest that the predominant proteins in SARS-CoV are characterized by about 46 kDa nucleocapsid protein and the 139 kDa spike proteins. Other proteins in reasonable abundance are E, M and N proteins.

[0056] Detection specificity of a target virus will require some level of background removal, particularly if the background contains other proteins. If large amounts of exogenous proteins are present, the peptide map could be dominated by non-target peptides. As previously described, affinity capture probes for the virus toxins based on glycan-decorated agarose beads may be used to readily clean up the toxins, even in large excess of background proteins, and other biomolecules. When analyzing exhaled breath for virus targets such as SARS-CoV-2, other human proteins (FIG. 6 and Example 3), in breath may interfere with detection specificity. An affinity-based cleanup of the sample is required to ensure highest specificity. Virus detection may require bead materials that provide more selective affinity compared to the glycan-decorated beads previously described. For example, dextran-based adsorbents may be used for purifying viruses, including coronaviruses, but the affinity of this resin for the target virus may not be satisfactory. As an alternative, carbohydrates may be used for viral and protein purification including target viruses such as SARS-CoV and SARS-CoV-2. Further heparin, and heparan sulfate may be used as binding agents bound to resin beads. Heparin covalently linked to sepharose beads (GE Healthcare Life Sciences, Heparin Sepharose 6 Fast Flow affinity resin Product #17099801) may be used instead of glycan capture beads. This resin may enable bead-based capture affinity capture system for collecting virus particles from exhaled breath. In an exemplary diagnostic system, exhaled breath samples may be pulled through a capture bed in a sample collection system 1000, collecting particles from the breath. The resin beads (bed) may be washed to remove any background material. The viral particles adsorbed to the beads would then be eluted off using high concentration of acid solution, such as at least one of about 12.5% acetic acid, about 5% TFA, about 5% formic acid and about 10% HCl, into the hot acid digestion chamber to generate the characteristic peptides. The peptide samples may be mixed with MALDI matrix and deposited onto as suitable substrate for MALDI TOFMS analysis. The samples may also be deposited on a suitable substrate or disk that is precoated with MALDI matrix.

[0057] FIG. 3 is a schematic diagram of an exemplary diagnostic method 3000 using exemplary system 2000. Exemplary method 3000 may be used to perform autonomous point-of-care diagnosis based on exhaled breath. In step 3001, the individual (or patient) may be directed to be seated; the chair may optionally be located in a containment booth. In step 3002, sample breath collection element 1007 may be removably fitted to the individual's head. The individual is then instructed to breathe or perform one or more predetermined maneuvers 3003 which may include a pre-set number of repetitions. Non-volatile organics in breath are captured using system 1000 and extracted using system 2002 in step 3004 and eluted using suitable solvents. During sample collection, human exhaled breath passes through the column at a predetermined flow rate drawn by a pulling pump. Since nonvolatile molecules contained in the exhaled breath interact with the functionalized beads in capture element 1001 (e.g. C18 functional groups immobilized on resin beads), these molecules are trapped in the column bed in element 1001 while hydrophilic molecules comprising mostly of water and aqueous electrolytes in the breath pass through the column. Nonvolatile organic molecules in human breath a show strong affinity for alkyl chains via intermolecular forces including hydrogen bond and noncovalent interaction. Elution of nonvolatile molecules from the column bed may be accomplished using organic solvent that include, but are not limited to, acetonitrile, methanol, and isopropanol as previously described. The sample may be further processed in step 3005 using component 2004. The type of sample processing depends on the type of diagnostic device and the non-volatile analyte particle of interest. As previously described, a virus sample may be subjected to hot acid digestion chamber to generate characteristic peptides. The peptide samples may be mixed with MALDI matrix and deposited onto as suitable substrate for MALDI TOFMS analysis. The samples may also be deposited on a suitable substrate or disk that is precoated with MALDI matrix. The sample is then analyzed by a diagnostic device in step 3006. When the diagnostic device is MALDI-TOFMS, sample processing may also comprise the steps of plating the sample on to a MALDI-TOFMS sample disk, heating the disk to concentrate the sample, and drying the disk. The sample disk is then analyzed using a MALDI-TOFMS. The TOFMS detectors may be modified to incorporate an ion gate and a reflectron to enable analysis and sequencing of COVID-19 type virus peptides that are fragmented during MALDI-TOF/MS. The spectrum obtained is compared to spectra from samples that were known positives to specific respiratory infections, to spectra in known databases, and also to spectra of samples form patients know to be healthy, and a diagnosis of the patient is generated. The result may then be communicated to a clinician or to the patient.

[0058] Once the breath collection element 1007 is attached to the patient, and sample extraction is initiated, the exemplary systems and methods may be preferably autonomous (with the exception of asking the patient to the leave the chair after performing the required maneuvers) and generates a test result of the diagnosis. In the case of virus particles like SARS-CoV-2, the particles are about 0.1 micron in diameter and sensitivities may be between about 10.sup.3 and 10.sup.4 viral particles.

[0059] Reports suggests that analysis of nose and throat swabs from influenza patients and COVID-19 patients produce viral counts of between about 10.sup.3 and 10.sup.10 viral particles. Less is known about the viral particles count in the breath of patients. Other reports suggest that influenza patients exhaled >10.sup.4 particles in about 30 minutes of breathing. If the output of SARS-CoV-2 is similar to that of influenza, an output of 10.sup.3 to 10.sup.4 particles in exhaled breath with a particle collection efficiency of >99.9% should be sufficient to identify the target virus particles in exhaled breath using the exemplary methods and systems disclosed herein. Detection time using the exemplary systems and methods may be between about 10 minutes and 20 minutes include the steps of sample extraction (breathing maneuvers), sample collection, sample processing (digestion) and analysis using a MALDI TOF-MS. This detection time is quite rapid compared to existing detection systems.

[0060] An exemplary sample processing component may comprise a hot acid digestion module or cartridge to autonomously extract sample from the packed bed column 1001, perform sample clean-up, conduct the hot acid digestion and provide a sample ready for plating on a MALDI-TOFS sample substrate or disk. The cartridge may be designed for reusability by adding the capability to flush the cartridge between uses.

[0061] Disclosed in another exemplary sample collection system 7000 (FIG. 7). Exemplary sample capture element 7001 may comprise a packed bed column comprising C18-bonded resin beads. These resin beads have C18 functional groups immobilized on the surface. Capture element 7001 may be connected or removably installed to a first aid CPR rescue mask 7007 with minor modifications. The stem 7008 of mask 7007 that usually connects to a resuscitation bag may be modified to removably connect to a HEPA filter 7009. The HEPA filter prevents contamination of exhaled breath by contaminants from ambient air. The oxygen inlet 7010 to the mask usually located below the stem and configured to be proximate to the chin of a human subject when a mask is worn by the subject may be modified to removably connect to capture element 7001. Element 7001 may be a removably inserted into mask 7007 through inlet 7010 or otherwise removably connected to or inserted into mask 7007 to form a substantially leak-tight fit with mask 7007. Mask 7007 may comprise elastic bands or ties that may be looped behind the head of a human subject to seal the mask to the face of the patient. Mask 7007 configured as described above prevents direct contact between the mouth and the inlet of the column in element 7001 and minimizes or eliminates contamination of the column inlet by saliva and also maximizes non-volatile organic particle collection from exhaled breath. Trap 7003 immersed in ice water may be installed after (downstream) of capture element 7001. The flow rate (air draw rate) using pump 7006 may be controlled using needle valve 7005 to pull about 600 mL/min. A nominal flow rate of between about 200 ml/min and 600 ml/min may be used. An optional HEPA filter 7011 may be installed between trap 7003 and needle valve 7005. Other fluidic components such as a check valve (see FIG. 1) may be installed in system 7000 to prevent backflow into the column bed in element 7001. CO.sub.2 in exhaled breath passes through the column bed in element 7001. To determine if exhaled breath sample volume and/or breathing maneuvers are adequate, a CO.sub.2 sensor may be disposed between the outlet of breath capture element 7001 and trap 7003. CO.sub.2 monitoring allows for an approximation of the proportion of exhaled air volume. A particle counter may also be installed between the outlet of element 7001 and trap 7003 to detect the size and number for particles exiting the column bed, which may also be used to detect saturation of the bed and breakthrough of nonvolatile organic molecules from the column bed. Exemplary system 7000 may also comprise a capture element 7001 bypass line (not shown) to enable standardization of breath volume prior to routing into the column bed in element 7001. A CO.sub.2 sensor and particle counter may also be fluidly connected to the bypass line. The capacity of solid beads immobilized with functional groups in the column bed in capture element 7001 to capture non-volatile organic molecules may be between about 0.05 mg (non-volatile organics)/mg beads and about 0.5 mg/mg. The capacity of C18-bonded resin beads in the column bed in exemplary capture element may be about 0.1 mg/mg. That is, a column bed having 25 mg C18 beads would have the capacity to trap or adsorb about 2.5 mg of non-volatile organic molecules.

[0062] Besides C18 functional groups, other functional groups that show affinity to nonvolatile molecules may be used as adsorbents in the column immobilized on solid phase beads such as resin beads. The solid phase beads may be made of polymers and particles such as resins, cellulose, silica, agarose, and hydrated Fe.sub.3O.sub.4 nanoparticles. Adsorbent materials may comprise other functional groups that include, but are not limited to, octadecyl, octyl, ethyl, cyclohexyl, phenyl, cyanopropyl, aminopropyl, 2,3-dihydroxypropoxypropyl, trimethyl-aminopropyl, carboxypropyl, benzenesulfonic acid, and propylsulfonic acid disposed on solid phase beads. Functional groups may also comprise at least one of ion exchange phases, polymer phases, antibodies, glycans, lipids, DNA and RNA.

[0063] The exemplary system and methods described herein are not necessarily limited in their diagnostic capability to respiratory infections. Lung cancer, for example, may also release biomarkers into the peripheral lung fluid, and these biomarkers would be readily detected by the systems and methods disclosed. Furthermore, because blood comes into intimate contact with the alveolar lining in the lungs, biomarkers of infection and cancer in other parts of the body (beyond the lungs) may be transferred across the alveolar lining and into the peripheral lung fluid, and thus, may be detected by the analysis of EBA. As a result, the scope of the invention is not limited to the detection and diagnosis of respiratory disease. The exemplary systems and methods may be used to capture aerosol chemical particles such a ricin and analyze the particles to prevent a chemical attack threat.

EXAMPLES

Example 1. Particle Capture Efficiency Using an Exemplary Packed Bed Column 1001

[0064] About 200 μL of HPLC-grade water was aerosolized into a 2-liter chamber using a portable Aeroneb Go nebulizer (Philips, Amsterdam, Netherlands) generating particles of size between about 0.3 μm to 5 μm in size. Column inlet particle size was measured. Particle counts were recorded using a portable laser particle counter (Met One Instruments, Grants Pass, Oreg.) under four test conditions: bare column having no particle bed, column having about 0.2 μm pore-size ×25 mm ID syringe filter (VWR International, Radnor, Pa.), a column 1001 comprising 30 mg of C18 resin beads (C18 column), and the column 1001 comprising C18 beads after 30 min. of run time (30 min incubation). The particle counter was located downstream of the column. The particle count for 0.3 μm particles was about 37,000 without the C18-packed column and dropped to about 480 with the C18-packed column (FIG. 4) indicating greater than 99% capture efficiency. A similar capture efficiency was observed for particles of other sizes studied. More importantly, the high capture efficiency was maintained after running the collection for about 30 min. For the 0.3-micron size bin (the most penetrating particle size for filters) the background counts in the laboratory were 146,000 particles/L. When the collection column was attached, the counts dropped to 14 particles/L, which is about a 99.99% collection efficiency (FIG. 4), equivalent or better than a HEPA filter.

Example 2. Analysis of Nonvolatile Organic Molecules Collected Using an Exemplary Packed Bed Column 1001 and MS

[0065] Based on size and chemical properties, nonvolatile organic molecules may be divided into three broad categories: small polar molecules, small nonpolar molecules (lipids), and macromolecules (peptides and proteins). To demonstrate the collection efficiency of these three types of molecules using exemplary column 1001 comprising a bed of C18 beads, representative molecules from each category were selected and characterized using high resolution mass spectrometry for the accurate mass measurement. 10 nM methadone (Sigma-Aldrich, St. Louis, Mo.) was selected to represent small polar molecules, 1,2-Dipalmitoyl-sn-glycero-3-phosphorylcholine (Matreya LLC, State College, Pa.) to represent lipids, and insulin from porcine pancreas (Sigma-Aldrich) to represent peptides and proteins. About 200 μL of each chemical prepared in HPLC-grade water was aerosolized using a portable Aeroneb Go nebulizer (Philips) into a 50 mL conical tube sitting on a heating block held at about 50° C. Column 1001 comprising about 30 mg of C18 beads was installed at the bottom of the 50 mL conical tube and the flow rate of pump 1006 was set as about 200 mL/min. The aerosolized chemical in each case was collected for about 5 minutes in the bed in column 1001. After collection of methadone and insulin, the collection column was washed four times with 400 μL of water. After quick centrifugation (which is an optional step), methadone and insulin were eluted using 400 μL of 70% acetonitrile. In the case of phosphorylcholine, the column was washed thrice with about 400 μL of 70% acetonitrile and elution (adsorbate extraction) was accomplished using 400 μL of 70% isopropanol. The washing solutions exiting the bed in each case was saved for analysis. Methadone and insulin collected (extracted) from the bed in column 1001 were lyophilized and re-suspended in 200 μL of 70% acetonitrile with 1% acetic acid. Phosphorylcholine collected from the bed in column 1001 was lyophilized and re-suspended in 200 μL of 50% isopropanol, 25% acetonitrile with 1% acetic acid. Mass spectrometric data of methadone, phosphorylcholine, and insulin were collected using an Orbitrap LTQ mass spectrometer (Thermo Fisher Scientific) via direct infusion in the positive ion mode. The flow rate of direct infusion was set to 3 μL/min and the data collection was recorded for 10 min at a resolution of about 60,000 at 200 m/z. Target molecules were identified by accurate mass measurement. As shown in FIG. 5, mass spectrometric signals for each representative molecule were only detected in the elution solution comprising molecules extracted from the C18 bed, but not in the washing solution. Exemplary column 1001 with a bed of C18 beads is therefore effective in collecting small polar molecules, small nonpolar molecules (lipids), and macromolecules (peptides and proteins) and is capable of high efficiency sample collection of these types of non-volatile organic molecules.

Example 3. Analysis of Exhaled Breath Collected from Human Subjects

[0066] In exemplary system 7000 previously described, the column in element 7001 comprised about 25 mg of C18 beads. Breath samples were collected from four healthy human subjects having different breathing volume with the same flow rate: subject 1 producing 144 L breathing volume, subject 2 producing 40 L, subject 3 and subject 4 producing 81 L. After breath sample collection, the collection column was removed and eluted with 400 μL of 70% acetonitrile for the collection of small polar molecules and proteins. The protein samples were lyophilized to remove the solvent and resuspended in 100 μL of 0.1% formic acid. Then, the column was eluted with 400 μL of 70% isopropanol for the collection of nonpolar molecules (lipids).

[0067] Samples were analyzed using SDS-PAGE electrophoresis and bottom-up proteomics. Electrophoresis was conducted using a Criterion Tris-HCl Gel system (Bio-Rad Laboratories, Hercules, Calif.) and using about 25 μL of the collected sample corresponding to each subject. After SDS-PAGE electrophoresis, the SDS-PAGE gel was treated with silver staining (Thermo Fisher Scientific) for the visualization of proteins. Bovine serum albumin (BSA) was used an internal positive control. During bottom-up proteomics, about 50 μL of total collected sample corresponding to each subject was treated as described herein. Briefly, about 50 μL of 50 mM ammonia bicarbonate (pH 8.5) was added to each sample. Protein reduction was conducted by adding dithiothreitol to the final concentration of 5 mM and incubated for 30 min at 37° C. After reduction, protein alkylation was followed by adding iodoacetamide to the final concentration of 15 mM and incubated for 1 h at room temperature. Trypsin (Thermo Fisher Scientific) was used for overnight protein digestion. After digestion, peptides were cleaned using C18-packed tips (Glygen, Columbia, Md.). Final peptide samples were constructed in 20 μL of 0.1% formic acid for mass spectrometry analysis. The samples were processed using an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled to a Q Exactive HF Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). During tandem mass spectrometry analysis, peptides were loaded into an Acclaim PepMap 100 C18 trap column (0.2 mm×20 mm, Thermo Fisher Scientific) with a flow rate of 5 μl/min and separated on an EASY-Spray HPLC Column (75 μm×150 mm, Thermo Fisher Scientific). HPLC gradient was conducted using 5-55% of the mobile phase (75% acetonitrile and 0.1% formic acid) with a flow rate of 300 nL/min for 60 minutes. Mass spectrometry data collection was conducted in the data-dependent acquisition mode. Precursor scanning resolution was set to 60,000 and product ion scanning resolution to 15,000. Product ion fragmentation was accomplished using high energy collision-induced disassociation (HCD) with about 27% of total energy. Bottom-up proteomics raw data files were processed with MaxQuant-Andromeda software against the UniProt human protein database/knowledgebase.

[0068] FIG. 6A shows gel electrophoresis silver staining images for the four subjects. “X” denotes the lane contained no sample and was used as an internal negative control. Protein bands were not observed in the condensate (EBC) samples collected in trap 7003. The darkness intensity of the protein bands suggests the protein content in all the samples was greater than 10 ng per protein band, which is adequate for sequential bottom-up proteomics. In addition, a similar protein band pattern for the human subjects may be observed as the most intense protein bands appear at between about 50 kDa and about 75 kDa (band #1), at about 37 kDa (band #2), and between about 10 kDa and about 15 kDa (band #3). By using tandem mass spectrometry, proteins corresponding to band #1 were identified as serum albumin and keratins, proteins corresponding to band #2 as zinc-alpha-2-glycoprotein, and proteins corresponding to band #3 as cystatin, dermcidin, and S100 proteins. In addition, the protein clusters at about 37 kDa (band #2) suggests that protein posttranslational modifications, presumably glycosylation, may occur. Bottom-up proteomics and protein database searching confirmed that this protein is zine-alpha-2-glycoprotein, a protein with four known N-linked glycosylation sites (UniProt knowledgebase). The results indicate that silver staining-based protein visualization coupled with bottom-up proteomics may be used to identify protein profiles in exhaled breath samples even with very low protein concentration. Further, the intensity of the protein bands may be correlated with the collected breath volume as subject 1, who producing about 144 liters of exhaled breath, showed the darkest protein bands and subject 2 (40 liters) showed the lightest bands. Differences in breathing manners or protocols may also affect aerosol particle product production from each subject, which could contribute to uneven protein distribution in different subjects. No specific instruction on the breathing manner was used in the above exercise. The similar protein pattern was further confirmed in the TIC profile using tandem mass spectrometry (FIG. 6B). By using different protein analysis techniques, the results strongly indicated that the column collection system was able to capture proteins from exhaled breath and the protein content in the breath samples encouraged the sequential protein identification using bottom-up proteomics. In addition, major proteins in saliva, such as alpha amylase, were not identified suggesting that the modifications to exemplary facial mask 7007 prevented direct contact with the subject's mouth and the capture element 7001. The exemplary breath collection system disclosed herein may be used to effectively capture proteins from the lower respiratory airways.

[0069] The similarity in protein patterns in exhaled breath collected from the four subjects was further confirmed by total ion chromatography (TIC) in LC-MS analysis as shown in FIG. 6B. Ion chromatography peak patterns were similar for the four subjects and subject 1 showed relatively more intense peaks similar to the more intense bands seen in the staining images in FIG. 6A.

[0070] Bottom-up proteomics was used for the identification of proteins in the collected exhaled breath samples. 197 proteins were identified from subject 1, 47 proteins from subject 2, 25 proteins from subject 3, and 64 proteins for subject 4. The protein identification numbers are consistent with the protein content in the samples as subject 1 had the most proteins identified. In total, 303 proteins were identified from the 4 subjects. The most abundant proteins identified based on spectral matching are listed in Table 1, and include cystatin-A, dermcidin, and several members in the S100 protein family.

TABLE-US-00001 TABLE 1 The top 20 proteins identified from the breath samples from the four subjects based on the abundance. Sequence Mol. Sequence coverage weight length MS/MS Accession ID Protein Identification [%] [kDa] (aa) count P01040 Cystatin-A OS = Homo sapiens OX = 9606 100 11 98 46 GN = CSTA PE = 1 SV = 1 P81605 Dermcidin OS = Homo sapiens OX = 9606 43.6 11 110 35 GN = DCD PE = 1 SV = 2 P31151 Protein S100-A7 OS = Homo sapiens OX = 88.1 11 101 28 9606 GN = S100A7 Q9NZT1 Calmodulin-like protein 5 OS = Homo 95.9 16 146 26 sapiens OX = 9606 P62987 Ubiquitin-60S ribosomal protein L40 OS = 57.8 15 128 23 Homo sapiens OX = 9606 GN = UBA52 PE = 1 SV = 2; sp P00441 Superoxide dismutase [Cu—Zn] OS = 99.4 16 154 17 Homo sapiens OX = 9606 P25311 Zinc-alpha-2-glycoprotein OS = Homo sapiens 41.6 34 298 12 OX = 9606 P31944 Caspase-14 OS = Homo sapiens OX = 9606 38.8 28 242 11 GN = CASP14 PE = 1 P01036 Cystatin-S OS = Homo sapiens OX = 9606 59.6 16 141 11 GN = CST4 PE = 1 SV = 3 P02768 Serum albumin OS = Homo sapiens OX = 9606 14.6 69 609 10 GN = ALB PE = 1 P0DP25 Calmodulin-3 OS = Homo sapiens OX = 9606 38.3 17 149 9 GN = CALM3 PE = 1 P04264 Keratin, type II cytoskeletal 1 OS = Homo 18.3 66 644 9 sapiens OX = 9606 Q6UWP8 Suprabasin OS = Homo sapiens OX = 9606 20.7 61 590 9 GN = SBSN PE = 1 SV = 2 P02538 Keratin, type II cytoskeletal 6A OS = Homo 15.1 60 564 7 sapiens OX = 9606 P13645 Keratin, type I cytoskeletal 10 OS = Homo 7.4 59 584 6 sapiens OX = 9606 P31025 Lipocalin-1 OS = Homo sapiens OX = 9606 19.3 19 176 5 GN = LCN1 PE = 1 O14529 Homeobox protein cut-like 2 OS = Homo sapiens 1.9 162 1486 4 OX = 9606 Q6E0U4 Dermokine OS = Homo sapiens OX = 9606 10.5 47 476 4 GN = DMKN PE = 1 Q08554 Desmocollin-1 OS = Homo sapiens OX = 9606 5 100 894 4 GN = DSC1 PE = 1 Q02413 Desmoglein-1 OS = Homo sapiens OX = 9606 6 114 1049 4 GN = DSG1 PE = 1

TABLE-US-00002 TABLE 2 Proteins corresponding to the breath samples from the four subjects. Sequence Mol. Sequence coverage weight length MS/MS Accession Protein Identification [%] [kDa] (aa) count P01040 HUMAN Cystatin-A OS = Homo sapiens OX = 9606 100 11 98 46 GN = CSTA PE = 1 SV = 1 P00441 HUMAN Superoxide dismutase [Cu—Zn] OS = Homo 99.4 16 154 17 sapiens OX = 9606 GN = SOD1 PE = 1 SV = 2 P25311 HUMAN Zinc-alpha-2-glycoprotein OS = Homo sapiens 41.6 34 298 12 OX = 9606 GN = AZGP1 PE = 1 SV = 2 P01036 HUMAN Cystatin-S OS = Homo sapiens OX = 9606 59.6 16 141 11 GN = CST4 PE = 1 SV = 3 P02768 HUMAN Serum albumin OS = Homo sapiens OX = 9606 14.6 69 609 10 GN = ALB PE = 1 SV = 2 P04264 HUMAN Keratin, type II cytoskeletal 1 OS = Homo 18.3 66 644 9 sapiens OX = 9606 GN = KRT1 PE = 1 SV = 6; P13645 HUMAN Keratin, type I cytoskeletal 10 OS = Homo 7.4 59 584 6 sapiens OX = 9606 GN = KRT10 PE = 1 SV = 6; P31025 HUMAN Lipocalin-1 OS = Homo sapiens OX = 9606 19.3 19 176 5 GN = LCN1 PE = 1 SV = 1; sp Q01469 HUMAN Fatty acid-binding protein 5 OS = Homo sapiens 23.7 15 135 4 OX = 9606 GN = FABP5 PE = 1 SV = 3 Q04695 HUMAN Keratin, type I cytoskeletal 17 OS = Homo 11.1 48 432 4 sapiens OX = 9606 GN = KRT17 PE = 1 SV = 2; P06702 HUMAN Protein S100-A9 OS = Homo sapiens OX = 9606 28.9 13 114 4 GN = S100A9 PE = 1 SV = 1 P29508 HUMAN Serpin B3 OS = Homo sapiens OX = 9606 13.1 45 390 4 GN = SERPINB3 PE = 1 SV = 2 P10599 HUMAN Thioredoxin OS = Homo sapiens OX = 9606 50.5 12 105 4 GN = TXN PE = 1 SV = 3 P04040 HUMAN Catalase OS = Homo sapiens OX = 9606 9.1 60 527 3 GN = CAT PE = 1 SV = 3 P01037 HUMAN Cystatin-SN OS = Homo sapiens OX = 9606 33.3 16 141 3 GN = CST1 PE = 1 SV = 3 P04406 HUMAN Glyceraldehyde-3-phosphate dehydrogenase 15.2 36 335 3 OS = Homo sapiens OX = 9606 GN = GAPDH PE = 1 SV = 3 P12273 HUMAN Prolactin-inducible protein OS = Homo sapiens 19.9 17 146 3 OX = 9606 GN = PIP PE = 1 SV = 1 P04075 HUMAN Fructose-bisphosphate aldolase A OS = Homo 10.7 39 364 2 sapiens OX = 9606 GN = ALDOA PE = 1 SV = 2 P07355 HUMAN Annexin A2 OS = Homo sapiens OX = 9606 6.8 39 339 2 GN = ANXA2 PE = 1 SV = 2; sp P01833 HUMAN Polymeric immunoglobulin receptor OS = Homo 3.4 83 764 2 sapiens OX = 9606 GN = PIGR PE = 1 SV = 4 Q06830 HUMAN Peroxiredoxin-1 OS = Homo sapiens OX = 9606 13.1 22 199 2 GN = PRDX1 PE = 1 SV = 1 P32119 HUMAN Peroxiredoxin-2 OS = Homo sapiens OX = 9606 16.7 22 198 2 GN = PRDX2 PE = 1 SV = 5 P63104 HUMAN 14-3-3 protein zeta/delta OS = Homo sapiens 5.7 28 245 1 OX = 9606 GN = YWHAZ PE = 1 SV = 1 P01011 HUMAN Alpha-1-antichymotrypsin OS = Homo sapiens 7.1 48 423 1 OX = 9606 GN = SERPINA3 PE = 1 SV = 2 P17174 HUMAN Aspartate aminotransferase, cytoplasmic OS = 3.4 46 413 1 Homo sapiens OX = 9606 GN = GOT1 PE = 1 SV = 3 P30838 HUMAN Aldehyde dehydrogenase, dimeric NADP-preferring 2.6 50 453 1 OS = Homo sapiens OX = 9606 GN = ALDH3A1 SV = 3 P04083 HUMAN Annexin A1 OS = Homo sapiens OX = 9606 4.6 39 346 1 GN = ANXA1 PE = 1 SV = 2 P05089 HUMAN Arginase-1 OS = Homo sapiens OX = 9606 4.7 35 322 1 GN = ARG1 PE = 1 SV = 2 Q13867 HUMAN Bleomycin hydrolase OS = Homo sapiens OX = 3.5 53 455 1 9606 GN = BLMH PE = 1 SV = 1 P13987 HUMAN CD59 glycoprotein OS = Homo sapiens OX = 8.6 14 128 1 9606 GN = CD59 PE = 1 SV = 1 P23528 HUMAN Cofilin-1 OS = Homo sapiens OX = 9606 7.2 19 166 1 GN = CFL1 PE = 1 SV = 3 P54108 HUMAN Cysteine-rich secretory protein 3 OS = Homo 5.7 28 245 1 sapiens OX = 9606 GN = CRISP3 PE = 1 SV = 1 P04080 HUMAN Cystatin-B OS = Homo sapiens OX = 9606 24.5 11 98 1 GN = CSTB PE = 1 SV = 2 P28325 HUMAN Cystatin-D OS = Homo sapiens OX = 9606 9.9 16 142 1 GN = CST5 PE = 1 SV = 1 Q15828 HUMAN Cystatin-M OS = Homo sapiens OX = 9606 7.4 17 149 1 GN = CST6 PE = 1 SV = 1 P09228 HUMAN Cystatin-SA OS = Homo sapiens OX = 9606 23.4 16 141 1 GN = CST2 PE = 1 SV = 1 Q8TDM6 HUMAN Disks large homolog 5 OS = Homo sapiens OX = 1 214 1919 1 9606 GN = DLG5 PE = 1 SV = 4 P78417 HUMAN Glutathione S-transferase omega-1 OS = Homo 7.5 28 241 1 sapiens OX = 9606 GN = GSTO1 PE = 1 SV = 2 P04792 HUMAN Heat shock protein beta-1 OS = Homo sapiens 11.7 23 205 1 OX = 9606 GN = HSPB1 PE = 1 SV = 2 P35527 HUMAN Keratin, type I cytoskeletal 9 OS = Homo sapiens 3.4 62 623 1 OX = 9606 GN = KRT9 PE = 1 SV = 3; P35908 HUMAN Keratin, type II cytoskeletal 2 epidermal OS = 3.9 65 639 1 Homo sapiens OX = 9606 GN = KRT2 PE = 1 SV = 2;; P30086 HUMAN Phosphatidylethanolamine-binding protein 1 OS = 8 21 187 1 Homo sapiens OX = 9606 GN = PEBP1 PE = 1 SV-3 P30041 HUMAN Peroxiredoxin-6 OS = Homo sapiens OX = 9606 10.3 25 224 1 GN = PRDX6 PE = 1 SV = 3 P25789 HUMAN Proteasome subunit alpha type-4 OS = Homo sapiens 3.4 29 261 1 OX = 9606 GN = PSMA4 PE = 1 SV = 1 P06454 HUMAN Prothymosin alpha OS = Homo sapiens OX = 9606 15.3 12 111 1 GN = PTMA PE = 1 SV = 2 O00391 HUMAN Sulfhydryl oxidase 1 OS = Homo sapiens OX = 1.5 83 747 1 9606 GN = QSOX1 PE = 1 SV = 3 Q8NFJ5 HUMAN Retinoic acid-induced protein 3 OS = Homo sapiens 3.9 40 357 1 OX = 9606 GN = GPRC5A PE = 1 SV = 2 P31949 HUMAN Protein S100-A11 OS = Homo sapiens OX = 9606 10.5 12 105 1 GN = S100A11 PE = 1 SV = 2 P60174 HUMAN Triosephosphate isomerase OS = Homo sapiens OX = 3.8 31 286 1 9606 GN = TPI1 PE = 1 SV = 3 P35030 HUMAN Trypsin-3 OS = Homo sapiens OX = 9606 GN = 4.3 33 304 1 PRSS3 PE = 1 SV = 2 Q13404 HUMAN Ubiquitin-conjugating enzyme E2 variant 1 OS = Homo 8.2 16 147 1 sapiens OX = 9606 GN = UBE2V1 PE = 1 SV = 2 P14618 HUMAN Pyruvate kinase PKM OS = Homo sapiens OX = 9606 2.1 58 531 1 GN = PKM PE = 1 SV = 4; sp O95613 HUMAN Pericentrin OS = Homo sapiens OX = 9606 GN = 0.3 378 3336 1 PCNT PE = 1 SV = 4 Q96FV2 HUMAN Secemin-2 OS = Homo sapiens OX = 9606 GN = 7.3 47 425 1 SCRN2 PE = 1 SV = 3 Q07955 HUMAN Serine/arginine-rich splicing factor 1 OS = Homo 12.1 28 248 1 sapiens OX = 9606 GN = SRSF1 PE = 1 SV = 2 O14979 HUMAN Heterogeneous nuclear ribonucleoprotein D-like OS = 1 6 6 1 Homo sapiens OX = 9606 GN = HNRNPDL PE = 1 SV = 3 P08727 HUMAN Keratin, type I cytoskeletal 19 OS = Homo sapiens 1 2 1.8 1 OX = 9606 GN = KRT19 PE = 1 SV = 4;;; sp Q14152 HUMAN Eukaryotic translation initiation factor 3 subunit A 1.3 167 1382 1 OS = Homo sapiens OX = 9606 GN = EIF3A PE = 1 SV = 1 P15586 HUMAN N-acetylglucosamine-6-sulfatase OS = Homo sapiens 2.4 62 552 1 OX = 9606 GN = GNS PE = 1 SV = 3 Q96T58 HUMAN Msx2-interacting protein OS = Homo sapiens OX = 1 1 0.6 1 9606 GN = SPEN PE = 1 SV = 1 P00734 HUMAN Prothrombin OS = Homo sapiens OX = 9606 GN = 1 3 3.1 1 F2 PE = 1 SV = 2 Q687X5 HUMAN Metalloreductase STEAP4 OS = Homo sapiens OX = 1 4 4.1 1 9606 GN = STEAP4 PE = 1 SV = 1 P23470 HUMAN Receptor-type tyrosine-protein phosphatase gamma OS = 1 0 0 1 Homo sapiens OX = 9606 GN = PTPRG PE = 1 SV = 4

[0071] Nonvolatile organic molecules contained in exhaled air could originate from both upper and lower respiratory airways. To reveal the tissue origin of the proteins identified in the study described above, the identified proteins were compared with five published proteome databases from bronchoalveolar lavage fluid (BALF). It is well known that BALF breath sampling methods produce proteins from the origin of lower respiratory airways. The comparison showed that about 63 proteins identified in the above described study (Table 2) were reported in BALF proteomics databases, suggesting that the exemplary breath sample collection system and method disclosed herein was effective in capturing proteins that originate from the lower respiratory airways such as lung tissues. No proteins were identified that correlated with either bacteria or viruses suggesting that the volunteers were indeed healthy. Therefore, the exemplary methods and systems disclosed herein may be used as a diagnostic tool for detection of respiratory diseases based on identification of proteins in exhaled breath.

Example 4. Capture and Analysis of Aerosolized Bacteria and Virus Using an Exemplary Packed Bed Column 1001 and MALDI TOF-MS

[0072] One virus sample, Bacteriophage MS2, and three bacteria, Escherichia coli (E. coli), Pseudomonas fluorescens, and Yersinia rohdei were acquired from American Type Culture Collection (ATCC, Manassas, Va.). 5 μL of each sample was prepared in 200 of HPLC-grade water and aerosolized using a portable Aeroneb Go nebulizer (Philips) into a 50 mL tube. The collection column which comprises 25 g to 35 g of C18 beads was fluidly connected near the bottom of the 50 mL tube and a pump was used to draw the nebulized sample into and through the column. The flow rate of the pump was set at about 200 mL/min and the collection time was about 10 minutes. After the conclusion of the collection step, the collection column was washed with 400 μL of water 4 times. Subsequently, the column was eluted with 400 μL of 70% isopropanol. Both washing solution and elution solution were collected and saved for MALDI-TOFMS analysis.

[0073] CHCA (α-Cyano-4-hydroxycinnamic acid) MALDI matrix was prepared in about 50% acetonitrile containing about 0.1% TFA (trifluoro acetic acid) at a concentration of about 10 mg/mL. About 1.5 μL of sample was mixed with about 0.5 CHCA matrix and applied to a MALDI plate. In each case, after the sample was sufficiently dried, the plate was inserted into an Axima-CFR time-of-flight instrument (Kratos Analytical by Shimadzu Biotech, Manchester, U.K.). MALDI-TOF mass spectra were obtained in linear mode with a 337 nm N2 laser (laser power, 90 arbitrary units) and all spectra were collected as an average of 200 profiles from 1000-15,000 m/z.

[0074] FIG. 8A shows MALDI mass spectra of the whole bacterial cell analysis of control sample, elution sample, and washing sample of E. coli (left), Pseudomonas fluorescens (middle), and Yersinia rohdei (right). The bacterial signatures are clearly seen in the elution samples but not in washing samples, indicating that the collection column was able to capture aerosolized bacteria. FIG. 8B shows (A) MALDI mass spectra of the whole virus MS2 analysis and (B) MALDI mass spectra of hot acid digestion analysis of virus MS2. Full mass of MS2 capsid protein (P03612) and its doubly charged ion were observed with MALDI-TOF MS. After hot acid digestion, the intact capsid protein (red dot) was observed as well as its digested peptides, which were characterized by MALDI-TOF MS.

[0075] The Abstract is provided to comply with 37 C.F.R. § 1.72(b), to allow the reader to determine quickly from a cursory inspection the nature and gist of the technical disclosure. It should not be used to interpret or limit the scope or meaning of the claims.

[0076] Although the present disclosure has been described in connection with the preferred form of practicing it, those of ordinary skill in the art will understand that many modifications can be made thereto without departing from the spirit of the present disclosure. Accordingly, it is not intended that the scope of the disclosure in any way be limited by the above description.

[0077] It should also be understood that a variety of changes may be made without departing from the essence of the disclosure. Such changes are also implicitly included in the description. They still fall within the scope of this disclosure. It should be understood that this disclosure is intended to yield a patent covering numerous aspects of the disclosure both independently and as an overall system and in both method and apparatus modes.

[0078] Further, each of the various elements of the disclosure and claims may also be achieved in a variety of manners. This disclosure should be understood to encompass each such variation, be it a variation of an implementation of any apparatus implementation, a method or process implementation, or even merely a variation of any element of these.

[0079] Particularly, it should be understood that the words for each element may be expressed by equivalent apparatus terms or method terms—even if only the function or result is the same. Such equivalent, broader, or even more generic terms should be considered to be encompassed in the description of each element or action. Such terms can be substituted where desired to make explicit the implicitly broad coverage to which this disclosure is entitled. It should be understood that all actions may be expressed as a means for taking that action or as an element which causes that action. Similarly, each physical element disclosed should be understood to encompass a disclosure of the action which that physical element facilitates.

[0080] In addition, as to each term used it should be understood that unless its utilization in this application is inconsistent with such interpretation, common dictionary definitions should be understood as incorporated for each term and all definitions, alternative terms, and synonyms such as contained in at least one of a standard technical dictionary recognized by artisans and the Random House Webster's Unabridged Dictionary, latest edition are hereby incorporated by reference.

[0081] Further, the use of the transitional phrase “comprising” is used to maintain the “open-end” claims herein, according to traditional claim interpretation. Thus, unless the context requires otherwise, it should be understood that variations such as “comprises” or “comprising,” are intended to imply the inclusion of a stated element or step or group of elements or steps, but not the exclusion of any other element or step or group of elements or steps. Such terms should be interpreted in their most expansive forms so as to afford the applicant the broadest coverage legally permissible.

REFERENCES

[0082] 1. B. Bake, P. Larsson, G. Ljungkvist, E. Ljungström, and A-C Olin, “Exhaled particles and small airways,” Respiratory Research (2019) 20:8. [0083] 2. Fennelly K. P., Martyny J. W., Fulton K. E., Orme I. M., Cave D. M., et al. (2004) Cough-generated aerosols of Mycobacterium tuberculosis: a new method to study infectiousness. Am J Respir Crit Care Med 169: 604-609. [0084] 3. Dina Hashoul and Hossam Haick, “Sensors for detecting pulmonary diseases from exhaled breath,” Eur. Respir. Rev. 2019; 28: 190011. [0085] 4. Hunt, J., “Exhaled breath condensate: An evolving tool for noninvasive evaluation of lung disease,” J. Allergy Clin. Immunol. 2002; 110:28-34. [0086] 5. Maria D. King, Andrew R. McFarland, “Bioaerosol Sampling with a Wetted Wall Cyclone: Cell Culturability and DNA Integrity of Escherichia coli Bacteria,” Aerosol Sci. Technol., 46:82-93, 2012. [0087] 6. James J. McDevitt, Petros Koutrakis, Stephen T. Ferguson, Jack M. Wolfson, M. Patricia Fabian, Marco Martins, Jovan Pantelic, and Donald K. Milton, “Development and Performance Evaluation of an Exhaled-Breath Bioaerosol Collector for Influenza Virus,” Aerosol Sci. Technol. 2013 Jan. 1; 47(4): 444-451. [0088] 7. Benjamin Patterson, Carl Morrow, Vinayak Singh, Atica Moosa, Melitta Gqada, Jeremy Woodward, Valerie Mizrahi, Wayne Bryden, Charles Call, Shwetak Patel, Digby Warner, Robin Wood, “Detection of Mycobacterium tuberculosis bacilli in bio-aerosols from untreated TB patients,” Gates Open Research 2018, 1:11. [0089] 8. Wood R., Morrow C., Barry C. E., III, Bryden W. A., Call C. J., Hickey A. J., et al.:

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