CHINESE HERBAL MEDICINE FORMULA AND ITS PREPARATION METHOD

Abstract

A Chinese herbal medicine formula and its preparation method are provided. The Chinese herbal medicine formula is made from the following raw materials in parts by weight: 8-12 parts of rose hydrosol, 1-3 parts of peppermint hydrosol, 28-42 parts of Chinese herbal medicine extract, 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions and 4-10 parts of composite antibacterial agent. The preparation method includes adding the rose hydrosol and the peppermint hydrosol to the Chinese herbal medicine extract to obtain a stock solution; diluting the stock solution to obtain a diluted solution; and adding the chelated platinum ions, the chelated selenium ions, and the composite antibacterial agent to the diluted solution, uniformly stirring to obtain the Chinese herbal medicine formula. The prepared Chinese herbal medicine formula can effectively kill bacteria, viruses, and fungi, has excellent killing effects, and will not cause harm to human body.

Claims

1. A Chinese herbal medicine formula, wherein the Chinese herbal medicine formula is made from following raw materials in parts by weight: 8-12 parts of rose hydrosol, 1-3 parts of peppermint hydrosol, 28-42 parts of Chinese herbal medicine extract, 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions and 4-10 parts of composite antibacterial agent; wherein a preparation method of the composite antibacterial agent comprises following steps: step (1), calcining a bentonite to obtain a calcined bentonite; cooling the calcined bentonite to room temperature to obtain a cooled bentonite; pulverizing the cooled bentonite to obtain a pulverized bentonite; and dispersing the pulverized bentonite into an ethanol aqueous solution to obtain a first mixed solution; adjusting a potential of hydrogen (pH) of the first mixed solution with hydrochloric acid to obtain an adjusted first mixed solution; adding 3-mercaptopropyltriethoxysilane into the adjusted first mixed solution to obtain a second mixed solution; stirring the second mixed solution to undergo a reaction to obtain a first product; and centrifuging, washing, and drying the first product to obtain a sulfhydryl bentonite; and step (2), adding dodecafluoroheptyl methacrylate and methacryloyloxyethyltrimethyl ammonium chloride into N,N-dimethylformamide and stirring well to obtain a mixture; then dispersing the sulfhydryl bentonite into the mixture, following by performing ultrasonic dispersion to obtain a dispersed solution; then adding a photoinitiator into the dispersed solution to undergo a reaction under an irradiation of a ultraviolet light to obtain a second product; finally, centrifuging the second product to obtain a centrifuged second product; washing the centrifuged second product with isopropanol to obtain a washed second product; and drying the washed second product to obtain the composite antibacterial agent.

2. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (1), a concentration of the ethanol aqueous solution is in a range of 50 weight (wt) %- 75 wt %, and the pH of the first mixed solution is adjusted to a range of 3-5.

3. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (1), a weight ratio of the bentonite to the ethanol aqueous solution and to the 3-mercaptopropyltriethoxysilane is in a range of 10:(30-50):(2-5).

4. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (1), conditions of the calcining comprise: 150 Celsius degrees ( C.)-300 C. of calcination temperature and 1 hour (h)-2 h of calcination time; reaction conditions of the stirring comprise nitrogen (N2) as a stirring reaction atmosphere, 30 C.-45 C. of stirring reaction temperature, and 5 h-8 h of stirring reaction time.

5. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (2), a weight ratio of the dodecafluoroheptyl methacrylate to the methacryloyloxyethyltrimethylammonium chloride to the N,N-dimethylformamide, and to the sulfhydryl bentonite is in a range of (4-7):(1-3):(30-50):10.

6. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (2), the photoinitiator is one or more selected from the group consisting of 2,2-dimethoxy-2-phenylacetophenone, 2-ethoxy-1,2-diphenylethanone, and 2-butoxy-1,2-diphenylethanone.

7. The Chinese herbal medicine formula as claimed in claim 1, wherein, in the step (2), a time of the ultrasonic dispersion is in a range of 15 minutes (min)-30 min, a main wavelength of the ultraviolet light is in a range of 360 nanometers (nm)-365 nm, an intensity of the ultraviolet light is in a range of 45 milliwatts per square centimeter (mW/cm.sup.2)-75 mW/cm.sup.2, a time of the irradiation is in a range of 60 min-90 min, and a temperature of the reaction is in a range of 35 C.-45 C.

8. The Chinese herbal medicine formula as claimed in claim 1, wherein a preparation method of the Chinese herbal medicine extract comprises following steps: weighing, in parts by weight, 1-2 parts of safflower, 8-12 parts of peach seed, 8-12 parts of forsythia fruit, 8-13 parts of chrysanthemum, 7-13 parts of perilla, 18-25 parts of Platycodon root, 8-11 parts of star anise, 9-14 parts of Aucklandia root, 7-12 parts of Agastache herba, 10-12 parts of Atractylodesrhizome, 10-12 parts of white Atractylodes, 9-13 parts of green tangerine peel, 10-12 parts of cloves, 4-6 parts of Sichuan pepper and 13-17 parts of tea leaves to obtain a Chinese herbal medicine mixture; adding the Chinese herbal medicine mixture into a reaction vessel; then adding 500 parts of deionized water into the reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture; and performing distillation extraction on the to-be-extracted Chinese herbal medicine mixture at a temperature in a range of 94 C.-97 C. for a time in a range of 1.5 h-3 h to obtain the Chinese herbal medicine extract.

9. A preparation method of a Chinese herbal medicine formula, comprising following steps: adding, in parts by weight, 8-12 parts of rose hydrosol and 1-3 parts of peppermint hydrosol into 28-42 parts of Chinese herbal medicine extract to obtain a stock solution; diluting the stock solution to obtain a diluted stock solution; adding 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions, and 4-10 parts of composite antibacterial agent into the diluted stock solution, and uniformly stirring to obtain the Chinese herbal medicine formula; wherein the composite antibacterial agent is prepared by following steps: step (1), calcining a bentonite to obtain a calcined bentonite, cooling the calcined bentonite to room temperature to obtain a cooled bentonite, pulverizing the cooled bentonite to obtain a pulverized bentonite, and dispersing the pulverized bentonite into an ethanol aqueous solution to obtain a first mixed solution; adjusting a pH of the first mixed solution with hydrochloric acid to obtain an adjusted first mixed solution; adding 3-mercaptopropyltriethoxysilane into the adjusted first mixed solution to obtain a second mixed solution; stirring the second mixed solution to undergo a reaction to obtain a first product; and centrifuging, washing, and drying the first product to obtain a sulfhydryl bentonite; and step (2), adding dodecafluoroheptyl methacrylate and methacryloyloxyethyltrimethyl ammonium chloride into N,N-dimethylformamide and stirring well to obtain a mixture; then dispersing the sulfhydryl bentonite into the mixture, following by performing ultrasonic dispersion to obtain a dispersed solution; then adding a photoinitiator into the dispersed solution to undergo a reaction under an irradiation of ultraviolet light to obtain a second product; finally, centrifuging the second product to obtain a centrifuged second product, washing the centrifuged second product with isopropanol to obtain a washed second product, and drying the washed second product to obtain the composite antibacterial agent.

10. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (1), a concentration of the ethanol aqueous solution is in a range of 50 wt %- 75wt %, and the pH of the first mixed solution is adjusted to a range of 3-5.

11. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (1), a weight ratio of the bentonite to the ethanol aqueous solution and to the 3-mercaptopropyltriethoxysilane is in a range of 10:(30-50):(2-5).

12. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (1), conditions of the calcining comprise: 150 C.-300 C. of calcination temperature, and 1 h-2 h of calcination time; reaction conditions of the stirring comprise: N.sub.2 as a stirring reaction atmosphere, 30 C.-45 C. of stirring reaction temperature, and 5 h-8 h of stirring reaction time.

13. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (2), a weight ratio of the dodecafluoroheptyl methacrylate to the methacryloyloxyethyltrimethyl ammonium chloride to the N,N-dimethylformamide, and to the sulfhydryl bentonite is in a range of (4-7):(1-3):(30-50):10.

14. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (2), the photoinitiator is one or more selected from the group consisting of 2,2-dimethoxy-2-phenylacetophenone, 2-ethoxy-1,2-diphenylethanone, and 2-butoxy-1,2-diphenylethanone.

15. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein, in the step (2), a time of ultrasonic dispersion is in a range of 15 min-30 min, a main wavelength of the ultraviolet light is in a range of 360 nm-365 nm, an intensity of the ultraviolet light is in a range of 45 mW/cm.sup.2-75 mW/cm.sup.2, a time of the irradiation is in a range of 60 min-90 min, and a temperature of the reaction is in a range of 35 C.-45 C.

16. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein a preparation method of the Chinese herbal medicine extract comprises following steps: weighing, in parts by weight, 1-2 parts of safflower, 8-12 parts of peach seed, 8-12 parts of forsythia fruit, 8-13 parts of chrysanthemum, 7-13 parts of perilla, 18-25 parts of Platycodonroot, 8-11 parts of star anise, 9-14 parts of Aucklandia root, 7-12 parts of Agastache herba, 10-12 parts of Atractylodes rhizome, 10-12 parts of white Atractylodes, 9-13 parts of green tangerine peel, 10-12 parts of cloves, 4-6 parts of Sichuan pepper and 13-17 parts of tea leaves to obtain a Chinese herbal medicine mixture; adding the Chinese herbal medicine mixture into a reaction vessel; then adding 500 parts of deionized water into the reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture; and performing distillation extraction on the to-be-extracted Chinese herbal medicine mixture at a temperature in a range of 94 C.-97 C. for a time in a range of 1.5 h-3 h to obtain the Chinese herbal medicine extract.

17. The preparation method of the Chinese herbal medicine formula as claimed in claim 9, wherein a dilution factor of the stock solution is in a range of 10-20 times.

18. A Chinese herbal medicine composition, wherein the Chinese herbal medicine composition is made from following raw materials: a rose hydrosol, a peppermint hydrosol, a Chinese herbal medicine extract, chelated platinum ions, chelated selenium ions, and a composite antibacterial agent.

19. The Chinese herbal medicine composition as claimed in claim 18, wherein the composite antibacterial agent is prepared by following steps: (1) calcining 10 g of bentonite at a temperature of 300 C. for 2 h to obtain a calcined bentonite; cooling the calcined bentonite to room temperature to obtain a cooled bentonite; pulverizing the cooled bentonite to obtain a pulverized bentonite; and dispersing the pulverized bentonite into 50 g of 70wt % ethanol aqueous solution to obtain a first mixed solution; adjusting a pH of the first mixed solution to 5 with hydrochloric acid to obtain an adjusted first mixed solution; adding 5 g of 3-mercaptopropyltriethoxysilane into the adjusted first mixed solution to obtain a second mixed solution; stirring the second mixed solution to undergo a reaction at 45 C. in a N.sub.2 atmosphere for 8 h to obtain a first product; and centrifuging, washing, and drying the first product to obtain a sulfhydryl bentonite; and (2) adding 7 g of dodecafluoroheptyl methacrylate and 3 g of methacryloyloxyethyl trimethyl ammonium chloride into 50 g of N,N-dimethylformamide and stirring well to obtain a mixture; then dispersing 10 g of the sulfhydryl bentonite into the mixture, following by performing ultrasonic dispersion for 30 min to obtain a dispersed solution; then adding 2-butoxy-1,2-diphenylethanone into the dispersed solution to undergo a reaction for 90 min under an irradiation of ultraviolet light with a main wavelength of 365 nm and an intensity of 60 mW/cm2 at a temperature of 45 C. to obtain a second product; finally, centrifuging the second product to obtain a centrifuged second product, washing the centrifuged second product with isopropanol to obtain a washed second product, and drying the washed second product to obtain the composite antibacterial agent.

20. The Chinese herbal medicine composition as claimed in claim 19, wherein the Chinese herbal medicine extract is prepared by following steps: weighing 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandia root, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves to obtain a Chinese herbal medicine mixture; adding the Chinese herbal medicine mixture into a reaction vessel; then adding 500 g of deionized water into the reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture; and performing distillation extraction on the to-be-extracted Chinese herbal medicine mixture at a temperature of 96 C. for 2.5 h to obtain the Chinese herbal medicine extract.

Description

DETAILED DESCRIPTION OF EMBODIMENTS

[0045] To make the objectives, technical solutions and advantages of the disclosure clearer, the disclosure will be further described in detail with reference to embodiments. It should be noted that, the specific embodiments described here are only used to explain the disclosure, and are not used to limit the disclosure.

[0046] Unless otherwise indicated, chemical reagents and materials in the disclosure are purchased by market means or synthesized from raw materials purchased by market means.

[0047] Bentonite is purchased from Haoyuan Bentonite Factory (Fangzi District, Weifang City, China), with a particle size of 4,000-5,000 mesh.

[0048] Both rose hydrosol and peppermint hydrosol are purchased from Guangzhou Zhonghe International Biotechnology Co., Ltd.

[0049] The chelated platinum ions and chelated selenium ions are purchased from Yatian Technology Group.

[0050] The disclosure will be further described with reference to specific embodiments.

Embodiment 1

[0051] A preparation method of a Chinese herbal medicine formula includes following four steps.

[0052] Step (1), 10 grams (g) of bentonite is calcined at a temperature of 300 C. for 2 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed into 50 g of 70wt % ethanol aqueous solution to obtain a first mixed solution, and a pH of the first mixed solution is adjusted to 5 with hydrochloric acid to obtain an adjusted first mixed solution. Then, 5 g of 3-mercaptopropyltricthoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred to undergo a reaction at 45 C. in a nitrogen (N.sub.2) atmosphere for 8 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain a sulfhydryl bentonite.

[0053] Step (2), 7 g of dodecafluoroheptyl methacrylate and 3 g of methacryloyloxyethyltrimethyl ammonium chloride are added into 50 g of N,N-dimethylformamide and stirred well to obtain a mixture. Then, 10 g of the sulfhydryl bentonite is dispersed into the mixture following by performing ultrasonic dispersion for 30 min to obtain a dispersed solution; then 2-butoxy-1,2-diphenylethanone is added into the dispersed solution to undergo a reaction for 90 min under an irradiation of ultraviolet light with a main wavelength of 365 nm and an intensity of 60 mW/cm.sup.2 at 45 C. to obtain a second product. Finally, the second product is centrifuged to obtain a centrifuged second product, the centrifuged second product is washed with isopropanol to obtain a washed second product, and the washed second product is dried to obtain a composite antibacterial agent.

[0054] Step (3), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandia root, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture; the Chinese herbal medicine mixture is added into a reaction vessel. Then, 500 g of deionized water is added into a reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0055] Step (4), 12 g of rose hydrosol and 3 g of peppermint hydrosol are added into 42 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted solution, 3 g of chelated platinum ions, 3 g of chelated selenium ions, and 10 g of the composite antibacterial agent are added into the diluted solution, and uniformly stirred to obtain the Chinese herbal medicine formula (i.e., a Chinese herbal medicine composition).

Embodiment 2

[0056] A preparation method of a Chinese herbal medicine formula includes following four steps.

[0057] Step (1), 10 g of bentonite is calcined at a temperature of 200 C. for 1.5 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed into 40 g of 70 wt % ethanol aqueous solution to obtain a first mixed solution; and a pH of the first mixed solution is adjusted to 4 with the hydrochloric acid to obtain an adjusted first mixed solution. Then 4 g of the 3-mercaptopropyltriethoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred to undergo reaction at 35 C. in the N2 atmosphere for 7 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain a sulfhydryl bentonite.

[0058] Step (2), 5 g of the dodecafluoroheptyl methacrylate and 2 g of the methacryloyloxyethyl trimethyl ammonium chloride are added into 40 g of the N,N-dimethylformamide and stirred well to obtain a mixture. Then 10 g of the sulfhydryl bentonite is dispersed into the mixture following by performing ultrasonic dispersion for 20 min to obtain a dispersed solution; then the 2-butoxy-1,2-diphenylethanone is added into the dispersed solution to undergo a reaction for 75 min under the irradiation of ultraviolet light with the main wavelength of 365 nm and the intensity of 60 mW/cm.sup.2 at 40 C. to obtain a second product. Finally, the second product is centrifuged to obtain a centrifuged second product; the centrifuged second product is washed with isopropanol to obtain a washed second product; and the washed second product is dried to obtain a composite antibacterial agent.

[0059] Step (3), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandia root, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture; the Chinese herbal medicine mixture is added into a reaction vessel. Then 500 g of deionized water is added into a reaction vessel with Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0060] Step (4), 10 g of rose hydrosol and 2 g of peppermint hydrosol are added into 35 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted solution, 2 g of chelated platinum ions, 2 g of chelated selenium ions, and 7 g of the composite antibacterial agent are added into the diluted solution, and uniformly stirred to obtain the Chinese herbal medicine formula.

Embodiment 3

[0061] A preparation method of a Chinese herbal medicine formula, includes following four steps.

[0062] Step (1), 10 g of bentonite is calcined at a temperature of 150 C. for 1 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed into 30 g of 70wt % ethanol aqueous solution to obtain a first mixed solution; and a pH of the first mixed solution is adjusted to 3 with hydrochloric acid to obtain an adjusted first mixed solution. Then 2 g of 3-mercaptopropyl triethoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred to undergo reaction at 30 C. in the N.sub.2 atmosphere for 5 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain a sulfhydryl bentonite.

[0063] Step (2), 4 g of dodecafluoroheptyl methacrylate and 1 g of methacryloyloxyethyl trimethyl ammonium chloride are added into 30 g of N,N-dimethylformamide and stirred well to obtain a mixture. Then 10 g of the sulfhydryl bentonite is dispersed into the mixture following by performing ultrasonic dispersion for 15 min to obtain a dispersed solution; then 2-butoxy-1,2-diphenylethanone is added into the dispersed solution to undergo a reaction for 60 min under the irradiation of ultraviolet light with the main wavelength of 365 nm and the intensity of 60 mW/cm.sup.2 at 35 C. to obtain a second product. Finally, the second product is centrifuged to obtain a centrifuged second product; the centrifuged second product is washed with isopropanol to obtain a washed second product; and the washed second product is dried to obtain a composite antibacterial agent.

[0064] Step (3), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandiaroot, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture; the Chinese herbal medicine mixture is added into a reaction vessel. Then 500 g of deionized water is added into a reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0065] Step (4), 8 g of rose hydrosol and 1 g of peppermint hydrosol are added into 28 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted stock solution, 1 g of chelated platinum ions, 1 g of chelated selenium ions, and 4 g of the composite antibacterial agent are added into the diluted stock solution, and uniformly stirred to obtain the Chinese herbal medicine formula.

Comparative Embodiment 1

[0066] A preparation method of a Chinese herbal medicine formula, includes following four steps.

[0067] Step (1), 10 g of bentonite is calcined at a temperature of 300 C. for 2 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed into 50 g of 70 wt % ethanol aqueous solution to obtain a first mixed solution, and a pH of the first mixed solution is adjusted to 5 with hydrochloric acid to obtain an adjusted first mixed solution. Then 5 g of 3-mercaptopropyltriethoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred to undergo a reaction at 45 C. in the N.sub.2 atmosphere for 8 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain a sulfhydryl bentonite.

[0068] Step (2), 7 g of dodecafluoroheptyl methacrylate is added into 50 g of N,N-dimethylformamide and stirred well to obtain a mixture. Then 10 g of the sulfhydryl bentonite is dispersed into the mixture following by performing ultrasonic dispersion for 30 min to obtain a dispersed solution; then 2-butoxy-1,2-diphenylethanone is added into the dispersed solution to undergo a reaction for 90 min under the irradiation of ultraviolet light with the main wavelength of 365 nm and the intensity of 60 mW/cm.sup.2 at 45 C. to obtain a second product. Finally, the second product is centrifuged to obtain a centrifuged second product; the centrifuged second product is washed with isopropanol to obtain a washed second product; and the washed second product is dried to obtain an antibacterial agent.

[0069] Step (3), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandiaroot, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture; the Chinese herbal medicine mixture is added into a reaction vessel. Then 500 g of deionized water is added into a reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0070] Step (4), 12 g of rose hydrosol and 3 g of peppermint hydrosol are added into 42 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted stock solution, 3 g of chelated platinum ions, 3 g of chelated selenium ions, and 10 g of the antibacterial agent are added into the diluted stock solution, and uniformly stirred to obtain the Chinese herbal medicine formula.

Comparative Embodiment 2

[0071] A preparation method of a Chinese herbal medicine formula, includes following four steps.

[0072] Step (1), 10 g of bentonite is calcined at a temperature of 300 C. for 2 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed to 50 g of 70wt % ethanol aqueous solution to obtain a first mixed solution, and a pH of the first mixed solution is adjusted to 5 with hydrochloric acid to obtain an adjusted first mixed solution. Then 5 g of 3-mercaptopropyltriethoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred to undergo reaction at 45 C. in the N2 atmosphere for 8 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain sulfhydryl bentonite.

[0073] Step (2), 3 g of methacryloyloxyethyl trimethyl ammonium chloride is added into 50 g of N,N-dimethylformamide and stirred well to obtain a mixture. Then 10 g of the sulfhydryl bentonite is dispersed into the mixture following by performing ultrasonic dispersion for 30 min to obtain a dispersed solution; then 2-butoxy-1,2-diphenylethanone is added into the dispersed solution to undergo a reaction for 90 min under the irradiation of ultraviolet light with the main wavelength of 365 nm and the intensity of 60 mW/cm.sup.2 at 45 C. to obtain a second product. Finally, the second product is centrifuged to obtain a centrifuged second product; the centrifuged second product is washed with isopropanol to obtain a washed second product; and the washed second product is dried to obtain an antibacterial agent.

[0074] Step (3), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandia root, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture, the Chinese herbal medicine mixture is added into a reaction vessel. Then 500 g of deionized water is added into a reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0075] Step (4), 12 g of rose hydrosol and 3 g of peppermint hydrosol are added into 42 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted stock solution, 3 g of chelated platinum ions, 3 g of chelated selenium ions, and 10 g of the antibacterial agent are added into the diluted solution, and uniformly stirred to obtain the Chinese herbal medicine formula.

Comparative Embodiment 3

[0076] A preparation method of a Chinese herbal medicine formula, includes following three steps.

[0077] Step (1), 10 g of bentonite is calcined at a temperature of 300 C. for 2 h to obtain a calcined bentonite, the calcined bentonite is cooled to room temperature to obtain a cooled bentonite, then the cooled bentonite is pulverized to obtain a pulverized bentonite. The pulverized bentonite is dispersed to 50 g of 70wt % ethanol aqueous solution to obtain a first mixed solution, and a pH of the first mixed solution is adjusted to 5 with hydrochloric acid to obtain an adjusted first mixed solution. Then 5 g of 3-mercaptopropyltriethoxysilane is added into the adjusted first mixed solution to obtain a second mixed solution, the second mixed solution is stirred and reacted at 45 C. in the N2 atmosphere for 8 h to obtain a first product, and the first product is centrifuged, washed, and dried to obtain a sulfhydryl bentonite.

[0078] Step (2), 2 g of safflower, 10 g of peach seed, 10 g of forsythia fruit, 11 g of chrysanthemum, 10 g of perilla, 22 g of Platycodon root, 9 g of star anise, 12 g of Aucklandiaroot, 8 g of Agastache herba, 11 g of Atractylodes rhizome, 11 g of white Atractylodes, 11 g of green tangerine peel, 11 g of cloves, 5 g of Sichuan pepper and 16 g of tea leaves are weighed to obtain a Chinese herbal medicine mixture; the Chinese herbal medicine mixture is added into a reaction vessel. Then 500 g of deionized water is added into a reaction vessel with the Chinese herbal medicine mixture to obtain a to-be-extracted Chinese herbal medicine mixture, and the to-be-extracted Chinese herbal medicine mixture is performed with distillation extraction at 96 C. for 2.5 h to obtain a Chinese herbal medicine extract.

[0079] Step (3), 12 g of rose hydrosol and 3 g of peppermint hydrosol are added into 42 g of the Chinese herbal medicine extract to obtain a stock solution; the stock solution is diluted 20 times to obtain a diluted stock solution, 3 g of chelated platinum ions, 3 g of chelated selenium ions, and 10 g of the sulfhydryl bentonite are added into the diluted stock solution, and uniformly stirred to obtain the Chinese herbal medicine formula.

[0080] Escherichia coli and Staphylococcus aureus are selected as experimental strains, and are activated in a broth medium at 37 C. for 8 hours with a shaker rotation speed of 150 revolutions per minute (rpm), and then are ready for use. Chinese herbal medicine formula products prepared in embodiments 1-3 and comparative embodiments 1-3, which have been sterilized at 121 C. for 20 minutes, are respectively added to a sterile broth medium at a concentration of 0.05% (weight/volume (w/v)). Under sterile conditions, 1 milliliter (mL) of activated bacterial solution (with a bacterial concentration of 109 colony-forming units per milliliter abbreviated as CFU/mL) is taken and added to 49 mL of mediums prepared as described above, and then is cultured at 37 C. for 6 h with a shaker rotation speed of 150 rpm to obtain a sample. After that, according to GB 4789.2-2022 National Food Safety Standard-Microbiological Examination of Food: Enumeration of Total Bacterial Colonies, samples after diluting are characterized by a plate count method. A bactericidal rate is calculated using following formula: Bactericidal Rate (%)=(Control Group Bacterial Count-Experimental Group Bacterial Count)/Control Group Bacterial Count100, where bacterial counts are expressed in CFU/mL. The test results are shown in Table 1.

TABLE-US-00001 TABLE 1 Bactericidal Rate of Chinese herbal medicine formulas 1 min 5 min 10 min Escherichia Staphylococcus Escherichia Staphylococcus Escherichia Staphylococcus coli aureus coli aureus coli aureus Embodiment 1 99.67 83.54 99.99 87.62 99.99 94.65 Embodiment 2 99.52 82.39 99.99 86.24 99.99 93.97 Embodiment 3 99.46 82.15 99.99 85.19 99.99 92.83 Comparative 76.71 61.32 81.27 64.53 83.64 69.48 Embodiment 1 Comparative 86.36 70.26 88.59 75.37 90.15 81.54 Embodiment 2 Comparative 72.93 52.91 76.52 59.46 81.52 65.32 Embodiment 3

[0081] Aforementioned descriptions are merely part of embodiments of the disclosure, but a scope of protection of the disclosure is not limited thereto. Within a technical scope disclosed by the disclosure, equivalent substitutions or alterations based on the technical solutions and inventive concept of the disclosure made by those skilled in the art fall within the scope of protection of the disclosure.