NON-TOXIC NANOMATERIAL FOR METALS EXTRACTION

Abstract

Fresh water contamination by heavy metals results from a variety of sources and can be damaging to wildlife, alter landscapes, and impact human health. metals removal form water sources is desirable for improving water quality and preventing adverse effects, but also for metals collection and recycling. Adsorption is a desirable metals extraction technique due to economic feasibility. Nanoscale materials exhibit high surface-area-to-volume ratio that lends to high adsorption and reactivity, making them ideal candidates for adsorptive metals extraction processes. Despite these properties, nanomaterials have elicited safety concerns. The extraordinarily small dimensions of these materials allow them to maneuver biological systems, tissues, and even cells, and combined with high reactivity, this translocation can result in toxic effects. It is therefore imperative that safety of nanomaterials for metals extraction be evaluated in addition to adsorptive properties. The current invention describes nanoparticles composed of magnetite, coated in hydroxyapatite, and functionalized for adsorption with titanium dioxide (TiHAMNPs). This material is safe, provides significant adsorption of metals, and allows efficient collection in magnetic systems.

Claims

1. A composition for the extraction of metals from the environment comprising TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs).

2. The composition for the extraction of metals from the environment of claim 1 wherein the precursors of said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) are comprised of deionized H.sub.2O, calcium hydroxide (Ca(OH).sub.2), magnetite NPs (Fe.sub.3O.sub.4), and titanium dioxide (TiO.sub.2) nanopowder, potassium phosphate (KH.sub.2PO.sub.4).

3. A method for extracting metals from the environment comprising the steps of: A. Addition of the TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) of claim 1 to a liquid that contains an environmental sample to produce a TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture; B. Manipulation of said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture to enable absorption of metals from said environmental sample to said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs); C. Collection of said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) containing an adsorbed metal.

4. The method for extracting metals from the environment of claim 3, further comprising the step of synthesizing said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) by wet chemical precipitation methods.

5. The method for extracting metals from the environment of claim 4, wherein said wet chemical precipitation methods is comprised of the following steps: A. adding an amount of deionized H.sub.2O and stirring; B. adding an amount of calcium hydroxide (Ca(OH).sub.2) to said deionized H.sub.2O and stirring until dissolved; C. adding an amount of magnetite NPs (Fe.sub.3O.sub.4) to said mixture of deionized H.sub.2O and calcium hydroxide (Ca(OH).sub.2) and stirring; D. adding an amount of titanium dioxide (TiO.sub.2) nanopowder to said mixture and stirring; E. dissolving an amount of potassium phosphate (KH.sub.2PO.sub.4) in a separate amount of deionized H.sub.2O to create a KH.sub.2PO.sub.4 solution and adding said solution to said mixture; F. reducing the pH of said mixture to approximately 7.5 to create a final solution; G. stirring said final solution; and H. centrifuging said final solution until a pellet of TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) has formed.

6. The method for extracting metals from the environment of claim 3, wherein said environmental sample is comprised of water.

7. The method for extracting metals from the environment of claim 3, wherein said environmental sample is comprised of soil.

8. The method for extracting metals from the environment of claim 3, wherein said environmental sample is comprised of biological materials.

9. The method for extracting metals from the environment of claim 3, wherein said absorbed metal is comprised of copper.

10. The method for extracting metals from the environment of claim 3, wherein said absorbed metal is comprised of a rare earth element.

11. The method for extracting metals from the environment of claim 3, wherein the manipulation step of said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture is comprised of any means to increase contact between said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) and metal ions of said mechanical mixing, agitation, stirring, shaking, and any other means, which would contact between metal ions of said environmental sample mixture.

12. The method for extracting metals from the environment of claim 3, wherein the manipulation step of said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture is comprised of an inline mixer.

13. The method for extracting metals from the environment of claim 3, wherein the collection step is comprised of the TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture travelling through a plurality of magnets.

14. The method for extracting metals from the environment of claim 3, wherein the addition, manipulation, and collection steps are contained within an inline pipe system.

15. The method for extracting metals from the environment of claim 3, further comprising an induced pressure means, whereby said induced pressure means force said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture through said addition, manipulation, and collection steps.

Description

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

[0010] FIG. 1. Scanning electron micrograph (A) and corresponding energy dispersive spectroscopy data (B) collected from TiHAMNPs. SEM images show TiHAMNPs were approximately round with diameters of 20-50 nm (A). Results from EDS show that TiHAMNPs are composed of expected elements including titanium and oxygen (titanium dioxide, TiO.sub.2), calcium, phosphate, and oxygen (hydroxyapatite, Ca.sub.5(PO.sub.4)(OH)), and iron and oxygen (magnetite, Fe.sub.3O.sub.4) (B). An aluminum sample stub (sample holder) and gold coating were used prior to imaging and have been removed from the EDS data graph. Resolution of the EDS system was not high enough to determine actual % composition of individual NPs, but rather was used to provide confirmation of bulk elemental composition and reveal if any unexpected elements were present (none were observed).

[0011] FIG. 2. Camera image of TiHAMNPs on the end of magnetic stir bar used to demonstrate magnetic properties of the NPs.

[0012] FIG. 3. X-ray diffraction (XRD) was used to determine the crystal structure and composition of TiHAMNPs. XRD data taken directly from the instrument software (A). Rwp for all three precursors compared to TiHAMNPs was 1.35%. A preferred Rwp or Rietveld refinement value is below 10%. XRD data graphed from raw data taken from the instrument (B). Graph shows relative intensities of the precursor materials and TiHAMNPs. Collectively, XRD indicated the three individual precursors were present in the final composite TiHMNP structure.

[0013] FIG. 4. Results from XTT assay with Vero cells. The XTT assay measured metabolic enzyme release, indicating healthy cellular activity. Metabolic activity was not significantly different between TiHAMNPs and the un-treated control (p=0.791), while cells exposed to SiMNPs experienced significant diminishment (p<0.001) metabolic activity.

[0014] FIG. 5. Zeta potential measurements of TiHAMNPs along with precursors and SiMNPs. Results show that the final TiO.sub.2 nanopowder coating of the particles increased zeta potential of the TiHAMNPs significantly. TiHAMNPs exhibited a slightly more neutral surface charge than SiMNPs. Neutral surface charge is relevant to preventing unwanted environmental reactions and cellular damage.

[0015] FIG. 6. Results of adsorption experiment. Results show that TiHAMNPs exhibited significantly higher adsorption than SiMNPs (p<0.001) and MNPs (p<0.001). After 24 hr, TiHAMNPs had reduced the amount of Cu in solution by a 73.67%, SiMNPs had reduced Cu by 30.39% and MNPs had reduced Cu by 14.05%.

[0016] FIG. 7. Preliminary tests were performed to examine removal of rare earth elements (REE's). In this adsorptive test, removal of Gd (heavy), Eu (heavy), Pr (light), and La (light) was monitored over 24 hr. Most promising, TiHAMNPs removed 97.22% Gd, 93.78% Eu, and 100% Pr, while leaving 23.47% La in solution after 60 min. The difference in collection efficiencies indicates potential for selective separation.

[0017] FIG. 8. Depiction of the in-line metals collection system. Outline A) shows the experimental setup used for magnetic collection. In these tests, a funnel was filled and used to control flow into the rest of the system. Outline B) shows the setup used for determining any loss of NPs that might occur in the system. *Note that the magnet holder was removed for these tests. The funnel was also used for loss experiments. Outline C) delineates the entire pipeline metals collection system used to determine total Cu recovery. In the metals collection system, NP and Cu solutions are poured into the system simultaneously by opening both valves at the same time. The solutions are combined and flow continues to a manipulation stage of the system. In this embodiment, the manipulation stage is comprised of a PVC inline mixer, which improves contact between metal ions and sorbent particles. Once the solution has been sufficiently manipulated, the solution travels to the collection stage of the system, wherein the metals are collected. Samples of the depleted solution were collected leaving the system and therefore provided a direct measurement of the Cu not collected by the NPs. Results showed a collection efficiency of 46.86% through the system at a flow rate of 103.4 cm/sec and an average residence time of 0.30 sec. Magnetic collection efficiency of the particles carrying metals was 89%, and loss of particles in the system (from sticking to walls or getting lost in any openings in the system) was negligible.

DETAILED DESCRIPTION OF THE INVENTION

[0018] TiHAMNPs (see FIGS. 1 and 2) were synthesized by wet chemical precipitation methods. Precursors were added in the order listed: [0019] 1) 200 mL deionized H.sub.2O (18 MΩ) was stirred by stir bar at room temperature (˜25° C.) at 275 rpm. [0020] 2) 0.260 g calcium hydroxide (Ca(OH).sub.2) was added to the stirred H.sub.2O until dissolved. [0021] 3) 0.243 g magnetite NPs (Fe.sub.3O.sub.4) (98+% purity, 20-30 nm diameter) was added to the Ca(OH).sub.2 solution and let stir for 1 min. [0022] 4) 0.263 g titanium dioxide (TiO.sub.2) nanopowder (anatase, 99.7% purity, <25 nm diameter) was added to the stirred solution and let stir for 1 min. [0023] 5) 0.408 g potassium phosphate (KH.sub.2PO.sub.4) was dissolved separately in 50 mL deionized H.sub.2O (18 MΩ). The KH.sub.2PO.sub.4 solution was then added dropwise over 2 min to the stirred solution. [0024] 6) pH of the solution after steps 1)-5) was measured at 10. A solution of 10% hydrochloric acid (HCl) in deionized (18 MΩ) H.sub.2O was used to reduce pH to 7.5. [0025] 7) The final solution was stirred for 1 wk at 25° C. and 275 rpm. [0026] 8) After 1 wk, TiHAMNPs were centrifuged at 2500 rpm for 10 min before supernatant fluid above the pellet was removed and replaced with deionized H.sub.2O (18 MΩ). Concentration of the particles was determined by drying in a weigh boat under constant ventilation and weighing. Final concentration of the TiHAMNPs is adjusted to around 3 mg/mL. [0027] 9) The final solution is autoclaved for 40 min to sterilize.

[0028] TiHAMNPs were characterized by FESEM and EDS to examine morphology and bulk composition. Results showed that TiHAMNPs were approximately 20-50 nm in diameter (FIG. 1, A). TiHAMNPs and SiMNPs were uniform across the samples examined. EDS analysis of the FESEMs for TiHAMNPs indicated all assumed elements were present, while any unexpected elements were not observed (FIG. 1, B). It was assumed that TiHAMNPs were composed of magnetite, HA, and TiO.sub.2 as distinct compounds making up the particle structure. This assumption was based on reports of HA forming on the surface of magnetite NPs (Mondal et al., 2017), and TiO.sub.2 dispersion on the surface of HA by simple stirring/heating methods (Lee et al., 2018).

[0029] X-Ray Diffraction analysis of TiHAMNPs as compared to the exact precursors used during synthesis revealed excellent fit with a Rietveld Refinement (Rwp) value of 1.35%. Peak values also showed a strong visual match between the three individual pre-cursors and final TiHAMNP structure, with little to no loss of significant peaks. This provided evidence of the layered NP structure as predicted, with each precursor maintaining its original form as added during synthesis (see FIG. 3).

[0030] Two common assays were used to examine the biocompatibility of TiHAMNPs. First, a lactate dehydrogenase (LDH) release assay was used. The LDH assay examines release of LDH which occurs due to cellular damage. In these tests, a positive control consisting of deliberately ruptured cells and a negative control consisting of cells grown normally in medium were used to calculate a % cytotoxicity of the test material. Results from these tests showed that at 1.0 g/L SiMNPs exhibited a cytotoxicity of 11.06%, while TiHAMNPs exhibited a cytotoxicity of 4.54% when exposed to a layer of African green monkey kidney cells (Vero). In the second assay, enzyme release from cells was monitored. This metabolic assay (XTT assay) allowed comparison of cell health between mammalian cells that had been exposed to NPs vs not been exposed to NPs (FIG. 4). In those tests, a statistically significant diminishment of cell health was observed when the mammalian cell line had been exposed to SiMNPs for 24 hr as compared to both TiHAMNPs. No statistically significant difference in cell health was observed between the healthy control cells and cells exposed to TiHAMNPs (p=0.791).

[0031] Results from these studies are encouraging and indicate high biocompatibility of TiHAMNPs. Importantly however, it should be noted that extended use of TiO2 NPs has raised concern due to nanotoxicity induced by oxidative stress under UV light, resulting in formation of reactive oxygen species (Rashid et al., 2021). Although we did not use these methods here, green nanotechnology methods have been and should be employed during synthesis of TiO2 as a precursor for TiHAMNP synthesis in the future. These processes are efficient, reduce the number of precursors required, and can employ the use of natural organisms such as plants, bacteria, or proteins for TiO2 NP synthesis (Irshad et al., 2021).

[0032] TiHAMNPs can be stored at room temperature and are stable as demonstrated by zeta potential measurements that show TiHAMNPs have a near neutral surface charge (see FIG. 5). Neutral surface charge is relevant to preventing unwanted environmental reactions and cellular damage.

[0033] Adsorption studies show that TiHAMNPs exhibit significantly higher adsorption of Cu after 24 hr as compared to SiMNPs (p<0.001) commonly used for metals collection, as well as magnetite NPs alone which have also been used (p<0.001) (see FIG. 6). This result provides good evidence that TiHAMNPs can be used not only as an effective metals collection material, but that TiHAMNPs are efficient adsorbers of metals (as compared to alternatives) after just 24 hr.

[0034] In addition to Cu removal, preliminary tests were performed to examine removal of rare earth elements (REE's). REE's are critical to production of high-tech materials, and industrial development in the areas of green energy and communications is becoming increasingly dependent on efficient extraction of these elements (Asadollahzadeh et al., 2020; Voncken, 2016). REE's have similar physical and chemical properties (Gupta and Krishnamurthy, 1992) and are difficult to separate at feasible cost. REE's are divided into light and heavy groups (Goodenough et al., 2018; Lima and Filho, 2016). In this adsorptive test, removal of Gd (heavy), Eu (heavy), Pr (light), and La (light) was monitored over 24 hr (FIG. 7). Most promising, TiHAMNPs removed 97.22% Gd, 93.78% Eu, and 100% Pr, while leaving 23.47% La in solution after 60 min (FIG. 7). The difference in collection efficiencies indicates potential for selective separation.

[0035] Testing of an in-line pipe metals collection system was performed by the addition of TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs) to a liquid that contains an environmental sample. The two solutions are combined just before a manipulation stage of the system to produce a TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs)-environmental sample mixture prior to introduction of the mixture to a manipulation stage of the system. The manipulation stage of the system is comprised of means for mechanical mixing, agitation, stirring, shaking, and any other means, which would increase contact between metal ions and sorbent particles (TiHAMNPs) to enable absorption of metals from the environmental sample to said TiO.sub.2-functionalized hydroxy apatite-coated magnetite nanoparticles (TiHAMNPs). Once the solution mixture has been sufficiently manipulated, the solution travels to the collection stage of the system, wherein metals are collected. In the embodiment described herein, the collection stage is comprised of a plurality of magnets, which the solution mixture travels through.

[0036] Total Cu recovery tests using the described in-line pipe metals collection system (FIG. 8) were performed by the addition of a Cu solution (1 L at 0.1 g/L) environmental sample and TiHAMNPs (1 L at 1.0 g/L) solution into the system simultaneously. The two solutions are combined to a mixture just before the manipulation stage of the system. In this embodiment, the manipulation stage of the system was comprised of a static mixer, which improves contact between metal ions and sorbent particles (TiHAMNPs). Other embodiments of the manipulation stage of the system include mechanical mixing, agitation, stirring, shaking, and any other means, which would contact between metal ions and sorbent particles. Once the solution mixture is sufficiently manipulated, the solution travels to the collection stage of the system, wherein the metals are collected. In the embodiment described herein, the collection stage is comprised of a plurality of magnets, which the solution travels through. Samples of the depleted solution mixture were collected leaving the system and therefore provided a direct measurement of the Cu not collected by the NPs. These collection values were subtracted from control values of Cu solutions prepared in the same manner and run through the system without the TiHAMNPs present. Results showed a collection efficiency of 46.86% through the system at a flow rate of 103.4 cm/sec and an average residence time of 0.30 sec.

[0037] In the above described in-line pipe metals collection system, gravitational pressure was used to force the aqueous Cu solution and TiHAMNPs mixture through the system. In industrial applications, such a system may be laid flat on the ground or earth in a horizontal configuration rather than a vertical configuration in order to lower environmental impact on the landscape among other reasons. In such an embodiment, the addition of induced pressure means by a mechanical pump, pressurized gas, or other similar induced pressure means, opposed to gravitational force, would be required to force sample through the metals collection pipeline.

[0038] It is understood that the foregoing examples are merely illustrative of the present invention. Certain modifications of the compositions and/or methods may be made and still achieve the objectives of the invention. Such modifications are contemplated as within the scope of the claimed invention.