PDE1 inhibitors for ophthalmic disorders
11464781 · 2022-10-11
Assignee
Inventors
Cpc classification
A61K31/5575
HUMAN NECESSITIES
A61K31/535
HUMAN NECESSITIES
International classification
A61K31/535
HUMAN NECESSITIES
A61K31/5575
HUMAN NECESSITIES
A61K9/00
HUMAN NECESSITIES
Abstract
Compounds that inhibit phosphodiesterase 1 (PDE1) are useful to treat glaucoma or elevated intraocular pressure. The PDE1 inhibitors may be administered as monotherapy or in combination with additional intraocular-pressure lowering agents. In addition, the invention provides ophthalmic compositions comprising PDE 1 inhibitors and optionally one or more additional intraocular pressure-lowering agents. Topical and systemic therapy may be used.
Claims
1. A method of treatment of glaucoma or elevated intraocular pressure comprising topically administering an effective amount of a PDE 1 inhibitor, or a combination of a PDE 1 inhibitor and an intraocular pressure-lowering agent, to the eye of a patient in need thereof wherein the PDE 1 inhibitor is the following compound: ##STR00039## in free or salt form.
2. The method according to claim 1, wherein a combination of the PDE1 inhibitor and an intraocular pressure-lowering agent is administered to the patient.
3. The method according to claim 2, wherein the intraocular pressure-lowering agent is an alpha adrenergic agonist, a beta-adrenergic antagonist, a prostaglandin or prostaglandin analog or a muscarinic agonist, a sympathomimetic, a miotic agent, a carbonic anhydrase inhibitor, a prostanoid, physostigmine, bimatoprost, brimonidine tartrate, or brimonidine tartrate/timolol maleate, or a combination thereof.
4. The method according to claim 2, wherein the prostaglandin is travoprost, latanoprost, bimatroprost, unoprostone, or unoprostone isopropyl.
5. The method according to claim 2, wherein the alpha adrenergic agonist is brimonidine, apraclonidine or dipivefrin.
6. The method according to claim 2, wherein the muscarinic agonist is pilocarpine.
Description
DETAILED DESCRIPTION OF THE INVENTION
(1) Compounds for Use in the Methods of the Invention
(2) The examples described and drawings rendered are illustrative and are not to be read as limiting any claim scope or claim construction.
(3) In one example, the PDE 1 Inhibitors for use in the methods of treatment described herein are a 7,8-dihydro-[1H or 2H]-imidazo[1,2-a]pyrazolo[4,3-e]pyrimidin-4(5H)-ones or 7,8,9-trihydro-[1H or 2H]-pyrimido[1,2-a]pyrazolo[4,3-e]pyrimidin-4(5H)-ones, of formula I:
(4) ##STR00001##
wherein (i) R.sub.1 is H or C.sub.1-4 alkyl (e.g., methyl); (ii) R.sub.4 is H or C.sub.1-4 alkyl and R.sub.2 and R.sub.3 are, independently, H or C.sub.1-4 alkyl (e.g., R.sub.2 and R.sub.3 are both methyl, or R.sub.2 is H and R.sub.3 is isopropyl), aryl, heteroaryl, (optionally hetero)arylalkoxy, or (optionally hetero)arylalkyl; or R.sub.2 is H and R.sub.3 and R.sub.4 together form a di-, tri- or tetramethylene bridge (pref. wherein the R.sub.3 and R.sub.4 together have the cis configuration, e.g., where the carbons carrying R.sub.3 and R.sub.4 have the R and S configurations, respectively); (iii) R.sub.5 is a substituted heteroarylalkyl, e.g., substituted with haloalkyl or R.sub.5 is attached to one of the nitrogens on the pyrazolo portion of Formula I and is a moiety of Formula Q
(5) ##STR00002## wherein X, Y and Z are, independently, N or C, and R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are independently H or halogen (e.g., Cl or F), and R.sub.10 is halogen, alkyl, cycloalkyl, haloalkyl (e.g., trifluoromethyl), aryl (e.g., phenyl), heteroaryl (e.g., pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl)), diazolyl, triazolyl, tetrazolyl, arylcarbonyl (e.g., benzoyl), alkylsulfonyl (e.g., methylsulfonyl), heteroarylcarbonyl, or alkoxycarbonyl; provided that when X, Y, or Z is nitrogen, R.sub.8, R.sub.9, or R.sub.10, respectively, is not present; and (iv) R.sub.6 is H, alkyl, aryl, heteroaryl, arylalkyl (e.g., benzyl), arylamino (e.g., phenylamino), heterarylamino, N,N-dialkylamino, N,N-diarylamino, or N-aryl-N-(arylakyl)amino (e.g., N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino); and (v) n=0 or 1; (vi) when n=1, A is —C(R.sub.13R.sub.14)— wherein R.sub.13 and R.sub.14, are, independently, H or C.sub.1-4 alkyl, aryl, heteroaryl, (optionally hetero)arylalkoxy or (optionally hetero)arylalkyl;
in free, salt or prodrug form, including its enatiomers, diasterisomers and racemates.
(6) The invention further provides the use of PDE 1 Inhibitors of Formula I as follows: 1.1 Formula I wherein R.sub.1 is methyl and n=0; 1.2 Formula I or 1.1 wherein R.sub.4 is H or C.sub.1-4 alkyl and at least one of R.sub.2 and R.sub.3 is lower alkyl, such that when the carbon carrying R.sub.3 is chiral, it has the R configuration, e.g., wherein both R.sub.2 and R.sub.3 are methyl, or wherein one is hydrogen and the other isopropyl; 1.3 Formula I or 1.1 wherein R.sub.4 is H and at least one of R.sub.2 and R.sub.3 is arylalkoxy; 1.4 Formula I wherein R.sub.1 is methyl, R.sub.2, R.sub.3, and R.sub.4 are H, n=1, and R.sub.13 and R.sub.14 are, independently, H or C.sub.1-4 alkyl (e.g., methyl or isopropyl); 1.5 Formula I or 1.1 wherein R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetramethylene bridge, having the cis configuration, preferably wherein the carbons carrying R.sub.3 and R.sub.4 have the R and S configurations respectively; 1.6 Formula I, 1.1 or 1.5 wherein R.sub.5 is a substituted heteroarylmethyl, e.g., para-substituted with haloalkyl; 1.7 Formula I, 1.1, 1.2, 1.3, 1.4 or 1.5 wherein R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are H and R.sub.10 is phenyl; 1.8 Formula I, 1.1, 1.2, 1.3, 1.4 or 1.5 wherein R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are H and R.sub.10 is pyridyl or thiadiazolyl; 1.9 Formula I, 1.1, 1.2, 1.3, 1.4 or 1.5 wherein R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are, independently, H or halogen, and R.sub.10 is haloalkyl; 1.10 Formula I, 1.1, 1.2, 1.3, 1.4 or 1.5 wherein R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are, independently, H, and R.sub.10 is alkyl sulfonyl; 1.11 any of the preceding formulae wherein R.sub.5 is attached to the 2-position nitrogen on the pyrazolo ring; 1.12 any of the preceding formulae wherein R.sub.6 is benzyl; 1.13 any of the preceding formulae wherein R.sub.6 is phenylamino or phenylalkylamino (e.g., benzylamino); 1.14 any of the preceding formulae wherein R.sub.6 is phenylamino; 1.15 any of the preceding formulae wherein X, Y, and Z are all C; 1.16 any of the preceding formulae wherein X, Y, and Z are all C and R.sub.10 is phenyl or 2-pyridyl; and/or 1.17 any of the preceding formulae wherein the compounds inhibit phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example 1; in free or salt form.
(7) For example, the PDE 1 Inhibitors include 7,8-dihydro-[1H or 2H]-imidazo[1,2-a]pyrazolo[4,3-e]pyrimidin-4(5H)-ones of Formula Ia:
(8) ##STR00003##
wherein (i) R.sub.1 is H or C.sub.1-4 alkyl [e.g., methyl]; (ii) R.sub.4 is H and R.sub.2 and R.sub.3 are, independently, H or C.sub.1-4 alkyl [e.g., R.sub.2 and R.sub.3 are both methyl, or R.sub.2 is H and R.sub.3 is isopropyl], aryl, or arylalkyl; or R.sub.2 is H and R.sub.3 and R.sub.4 together form a di-, tri- or tetramethylene bridge [pref. wherein the R.sub.3 and R.sub.4 have the cis configuration, e.g., where the carbons carrying R.sub.3 and R.sub.4 have the R and S configurations respectively]; (iii) R.sub.5 is attached to one of the nitrogens on the pyrazolo portion of formula Ia and is a substituted benzyl of formula B
(9) ##STR00004## wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are independently H or halogen (e.g., Cl or F); and R.sub.10 is halogen, alkyl, cycloalkyl, haloalkyl (e.g., trifluoromethyl), aryl (e.g., phenyl), heteroaryl (e.g., pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl)), arylcarbonyl (e.g., benzoyl), alkyl sulfonyl or heteroarylcarbonyl; and (iv) R.sub.6 is H, alkyl, aryl, heteroaryl, arylalkyl [e.g., benzyl], arylamino [e.g., phenylamino], heteroarylamino, arylalkylamino, N,N-dialkylamino, N,N-diarylamino, or N-aryl-N-(arylalkyl)amino [e.g. N-phenyl-N-(1,1′-biphen-4-ylmethyl)amino];
in free, salt or prodrug form.
(10) The invention further provides the use of PDE 1 Inhibitors of Formula Ia as follows: 2.1: Formula Ia wherein R.sub.1 is methyl; 2.2: Formula Ia or 2.1 wherein R.sub.4 is H and at least one of R.sub.2 and R.sub.3 is lower alkyl, such that when the carbon carrying R.sub.3 is chiral, it has the R configuration, e.g., wherein both R.sub.2 and R.sub.3 are methyl, or wherein one is hydrogen and the other isopropyl; 2.3: Formula Ia or 2.1 wherein R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetramethylene bridge, having the cis configuration, preferably wherein the carbons carrying R.sub.3 and R.sub.4 have the R and S configurations respectively; 2.4: Formula Ia, 2.1, 2.2 or 2.3 wherein R.sub.5 is a moiety of formula B wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are H and R.sub.10 is phenyl; 2.5: Formula Ia, 2.1, 2.2, or 2.3 wherein R.sub.5 is a moiety of formula B wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are H and R.sub.10 is pyridyl or thiadiazolyl; 2.6: Formula Ia, 2.1, 2.2, 2.3, 2.4, or 2.5 wherein R.sub.5 is attached to the 2-position nitrogen on the pyrazolo ring; 2.7: Formula Ia, 2.1, 2.2, 2.3, 2.4, 2.5 or 2.6 wherein R.sub.6 is benzyl; 2.8: Formula Ia, 2.1, 2.2, 2.3, 2.4, 2.5 or 2.6 wherein R.sub.6 is phenylamino or phenylalkylamino (e.g., benzylamino); and/or 2.9: Formula Ia, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, or 2.8 wherein the compounds inhibit phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example 1; in free or salt form.
(11) In an another embodiment, the PDE 1 Inhibitors are compounds of Formula I wherein (i) R.sub.1 is methyl; (ii) R.sub.2, R.sub.3 and R.sub.4 are H; (iii) n=1 and R.sub.a and R.sub.b are, independently, H or methyl; (iv) R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are H and R.sub.10 is phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); (v) R.sub.6 is benzyl, phenylamino or benzylamino; in free or salt form.
(12) In another embodiment, the PDE 1 Inhibitors are compounds of Formula I wherein (i) R.sub.1 is methyl; (ii) n=0; (iii) R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetra-methylene bridge [pref. with the carbons carrying R.sub.3 and R.sub.4 having the R and S configuration respectively]; or at least one of R.sub.2 and R.sub.3 is methyl, isopropyl or arylalkoxy and R.sub.4 is H; or R.sub.2 and R.sub.3 are H and R.sub.4 is a C.sub.1-4 alkyl; (iv) R.sub.5 is a substituted heteroarylmethyl, e.g., para-substituted with haloalkyl; or R.sub.5 is a moiety of Formula Q wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are H or halogen and R.sub.10 is haloalkyl, phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); and (v) R.sub.6 is benzyl, phenylamino or benzylamino; in free or salt form.
(13) In another embodiment, the PDE 1 Inhibitors are compounds of Formula Ia wherein (i) R.sub.1 is methyl; (ii) R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetra-methylene bridge [pref. with the carbons carrying R.sub.3 and R.sub.4 having the R and S configuration respectively]; or R.sub.2 and R.sub.3 are each methyl and R.sub.4 is H; or R.sub.2 and R.sub.4 are H and R.sub.3 is isopropyl [pref. the carbon carrying R.sub.3 having the R configuration]; (iii) R.sub.5 is a moiety of Formula B wherein R.sub.8, R.sub.9, R.sub.11, and R.sub.12 are H and R.sub.10 is haloalkyl, phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); and (iv) R.sub.6 is benzyl, phenylamino or benzylamino; in free or salt form.
(14) In another embodiment, the PDE 1 Inhibitors may be selected from the various formulae, as described in the subsequent paragraphs.
(15) For example, PDE 1 Inhibitors include compounds according to Formulae II, III and IV.
(16) ##STR00005##
wherein R.sub.a and R.sub.b are, independently, H or C.sub.1-4 alkyl; R.sub.6 is phenylamino or benzylamino; R.sub.10 is phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); in free or salt form.
(17) ##STR00006##
wherein R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetra-methylene bridge [pref. with the carbons carrying R.sub.3 and R.sub.4 having the R and S configuration respectively]; or at least one of R.sub.2 and R.sub.3 is methyl, isopropyl or arylalkoxy and R.sub.4 is H; or R.sub.2 and R.sub.3 are H and R.sub.4 is a C.sub.1-4 alkyl; R.sub.6 is phenylamino or benzylamino; R.sub.10 is haloalkyl, phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); in free or salt form.
(18) ##STR00007##
wherein R.sub.2 is H and R.sub.3 and R.sub.4 together form a tri- or tetra-methylene bridge [pref. with the carbons carrying R.sub.3 and R.sub.4 having the R and S configuration respectively]; or at least one of R.sub.2 and R.sub.3 is methyl, isopropyl or arylalkoxy and R.sub.a is H; or R.sub.2 and R.sub.3 are H and R.sub.4 is a C.sub.1-4 alkyl; R.sub.6 is phenylamino or benzylamino; R.sub.10 is phenyl, pyridyl (for example pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl); in free or salt form.
In a preferred embodiment, the PDE 1 Inhibitors for use in the methods of treatment described herein are a 1,3,5-substituted 6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-7-one, of formula V:
(19) ##STR00008##
wherein R.sub.a is methyl or C.sub.2-C.sub.6 alkyl; R.sub.1 is H or C.sub.1-C.sub.4 alkyl;
each of R.sub.2 and R.sub.3 is independently selected from H and C.sub.1-C.sub.4 alkyl, or R.sub.2 is H or C.sub.1-C.sub.4 alkyl and R.sub.3 is OH, C.sub.2-C.sub.4 alkanoyloxy or fluoro, or R.sub.2 and R.sub.3 when taken together represent C.sub.2-C.sub.6 alkylene, or R.sub.2 and R.sub.3 when taken together with the carbon atom to which they are attached represent a carbonyl group; Ar is either (a)
(20) ##STR00009##
wherein each of R.sub.4, R.sub.5 and R.sub.6 is independently selected from H C.sub.1-C.sub.4 alkyl, C.sub.1-C.sub.4 alkoxy, C.sub.1-C.sub.4 alkoxy-Z—, halo, halo(C.sub.1-C.sub.4)alkyl, phenoxy, optionally substituted by up to three substitutents each of which substitutent is independently selected from halo, C.sub.1-4 alkyl, and C.sub.1-C.sub.4 alkoxy, nitro, hydroxy, hydroxy-Z—, C.sub.2-C.sub.4 alkanoyl, amino, amino-Z—, (C.sub.1-C.sub.4 alkyl)NH, (C.sub.1-C.sub.4 alkyl).sub.2N—, (C.sub.1-C.sub.4 alkyl)NH—Z—, (C.sub.1-C.sub.4 alkyl).sub.2N—Z—, —COOH, —Z—COOH, —COO(C.sub.1-C.sub.4 alkyl), —Z—COO(C.sub.1-C.sub.4 alkyl) C.sub.1-C.sub.4 alkanesulphonamido, C.sub.1-C.sub.4 alkanesulphonamido-Z—, halo(C.sub.1-C.sub.4)alkanesulphonamido, halo(C.sub.1-C.sub.4)alkanesulphonamido-Z—, C.sub.1-C.sub.4 alkanamido, C.sub.1-C.sub.4 alkanamido-Z—, HOOC—Z—NH—, HOOC—Z—NH—Z—, (C.sub.1-C.sub.4 alkyl)OOC—Z—NH—, (C.sub.1-C.sub.4 alkyl)OOC—Z—NH—Z—, C.sub.1-C.sub.4 alkyl-NH—SO.sub.2—NH—, C.sub.1-C.sub.4 alkyl-NH—SO.sub.2—NH—Z—, (C.sub.1-C.sub.4 alkyl).sub.2-N—SO.sub.2—NH—, (C.sub.1-C.sub.4 alkyl).sub.2-N—SO.sub.2—NH—Z—, C.sub.1-C.sub.4 alkoxy CH═CH—Z—CONH—, C.sub.1-C.sub.4 alkoxy CH═CHCONH C.sub.1-C.sub.4 alkyl-SO.sub.2—N(C.sub.1-C.sub.4 alkyl)-, C.sub.1-C.sub.4 alkyl-SO.sub.2—N(C.sub.1-C.sub.4 alkyl)-Z—, (C.sub.1-C.sub.4 alkyl)NH—Z—SO.sub.2—NH—, (C.sub.1-C.sub.4 alkyl).sub.2N—Z—SO.sub.2—NH—Z—, (C.sub.1-C.sub.4 alkyl)NH—Z—SO.sub.2—NH—Z—, (C.sub.1-C.sub.4 alkyl).sub.2N—Z—SO.sub.2—NH—Z—, benzenesulphonamido, optionally ring substituted by up to three substitutents each of which is independently selected from halo, C.sub.1-4 alkyl, and C.sub.1-C.sub.4 alkoxy, C.sub.1-C.sub.4 alkanoyl-N(C.sub.1-C.sub.4 alkyl)-, C.sub.1-C.sub.4 alkanoyl-N(C.sub.1-C.sub.4 alkyl)-Z—, C.sub.1-C.sub.4 alkoxycarbonyl-CH(CH.sub.2OH)NHSO.sub.2—, —SO.sub.3H, —SO.sub.2NH.sub.2, H.sub.2NOC—CH(CH.sub.2OH)—NHSO.sub.2—, HOOC—Z—O—, and (C.sub.1-C.sub.4 alkyl)OOC—Z—O—, or optionally one of R.sub.4, R.sub.5 and R.sub.6 is a G-Het group and wherein the others of R.sub.4, R.sub.5 and R.sub.6 are independently selected from the R.sub.4, R.sub.5 and R.sub.6 substitutents listed above; Z is C.sub.1-C.sub.4 alkylene, G is a direct link, Z, O, —SO.sub.2NH—, SO.sub.2, or —Z—N(C.sub.1-C.sub.4 alkyl)SO.sub.2—, Het is a 5- or 6-membered heterocyclic group containing 1, 2, 3 or 4 nitrogen heteroatoms; or 1 or 2 nitrogen heteroatoms and 1 sulphur heteroatom or 1 oxygen heteroatom; or the heterocyclic group is furanyl or thiophenyl; wherein the Het group is saturated or partially or fully unsaturated and optionally substituted by up to 3 substitutents, wherein each substitutent is independently selected from C.sub.1-C.sub.4 alkyl, oxo, hydroxy, halo, and halo(C.sub.1-C.sub.4)alkyl; or (b) any one of the following bicyclic groups: benzodioxolanyl, benzodioxanyl, benzimidazolyl, quinolinyl, indolyl, quinazolinyl, isoquinolinyl, benzotriazolyl, benzofuranyl, benzothiophenyl, quinoxalinyl, or phthalizinyl, wherein said bicyclic Ar groups are linked to the neighbouring —C(R.sub.2R.sub.3)— group via the benzo ring portion, and wherein the heterocyclic portion of said bicyclic Ar group is optionally partially or fully saturated, said group being optionally substituted by one or more of C.sub.1-C.sub.4 alkyl, halo, hydroxy, oxo, amino, and C.sub.1-C.sub.4 alkoxy; or a pharmaceutically acceptable salt of the compound, or a pharmaceutically acceptable solvate of the compound or the salt.
(21) For example, PDE 1 Inhibitors for use in the present invention include 1,3,5,-substituted, 6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-7-one, in free or pharmaceutically acceptable salt form, particularly compounds of Formula V or the following formulae: 3.2 Of Formula V wherein R.sub.a is a C.sub.2-5 alkyl group; 3.3 Of Formula V wherein R.sub.a is a C.sub.2-4 alkyl group. 3.4 Of Formula V wherein R.sub.a is a C.sub.3 alkyl group. 3.5 Of Formula V wherein R.sub.a is methyl 3.6 Of Formula V, 3.2, 3.3, 3.4 or 3.5 wherein R.sub.1 is a C.sub.1-6 alkyl group. 3.7 Of any of the preceding formulae wherein R.sub.1 is a C.sub.1-3 alkyl group. 3.8 Of any of the preceding formulae wherein R.sub.1 is a methyl group. 3.9 Of any of the preceding formulae wherein R.sub.2 is H. 3.10 Of any of the preceding formulae wherein R.sub.3 is H. 3.11 Of any of the preceding formulae wherein R.sub.4, R.sub.5 and R.sub.6 are independently selected from H.sub.1 (C.sub.1-4 alkyl).sub.2N—, C.sub.1-4 alkanesulphonamido and benzenesulphonamido. 3.12 Of any of the preceding formulae wherein R.sub.4, R.sub.5 and R.sub.6 are independently selected from H, diethylamino, methanesulphonamido and benzenesulphonamido. 3.13 Of any of the preceding formulae wherein Ar is 4-diethylaminophenyl. 3.14 Of any of the preceding formulae wherein Ar is 2-methanesulphonamidophenyl. 3.15 Of any of the preceding formulae wherein Ar is 4-benzenesulphonamidophenyl. 3.16 Of any of the preceding formulae wherein one of R.sub.4, R.sub.5 and R.sub.6 is (C.sub.1-4 alkyl).sub.2N— and wherein the other two of R.sub.4, R.sub.5 and R.sub.6 are H. 3.17 Of any of the preceding formulae wherein one of R.sub.4, R.sub.5 and R.sub.6 is diethylamino and wherein the other two of R.sub.4, R.sub.5 and R.sub.6 are H. 3.18 Of any of the preceding formulae wherein R.sub.a is methyl. 3.19 Of any of the preceding formulae wherein R.sub.a is C.sub.2-C.sub.6 alkyl. 3.20 Of any of the preceding formulae wherein the compound is selected from the following:
(22) ##STR00010## 3.21 Of any of the preceding formulae wherein the compound is
(23) ##STR00011## 3.22 A compound which is a 1,3,5,-substituted, 6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-7-one, in free or pharmaceutically acceptable salt form, e.g. a compound of Formula V or according to any of formulae 3.2-3.21, wherein the compound inhibits phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example 1 below.
(24) In another embodiment, the PDE 1 Inhibitors for use in the methods of treatment described herein are substituted imidazo[2,1-b]purin-4-one of Formula VIIa or VIIb:
(25) ##STR00012##
in free, salt or prodrug form, including its enatiomers, diasterisomers and racemates, wherein: i) q=0, 1 or 2; ii) R.sup.1, R.sup.a, R.sup.b, R.sup.c and R.sup.d are each independently H, alkyl, aryl, heteroaryl, cycloalkyl or heterocycloalkyl groups, wherein each alkyl group of R.sup.1, R.sup.a, R.sup.b, R.sup.c and R.sup.d is independently unsubstituted or substituted with 1 to 5 independently selected R.sup.3 moieties which can be the same or different, each R.sup.3 moiety being independently selected from the group consisting of hydroxy, alkoxy, cycloalkoxy, aryloxy, alkylthio, arylthio, aryl, haloaryl, heteroaryl, cycloalkyl, heterocycloalkyl, amino, alkylamino, dialkylamino, cycloalkylamino and heterocycloalkylamino groups; wherein each of the aryl, heteroaryl, cycloalkyl and heterocycloalkyl groups of R.sup.1, R.sup.a, R.sup.b, R.sup.c and R.sup.d is independently unsubstituted or substituted with 1 to 5 independently selected R.sup.4 moieties which can be the same or different, each R.sup.4 moiety being independently selected from the group consisting of: halo, optionally substituted aryl (e.g., phenyl, chlorophenyl, methoxyphenyl), heteroaryl (e.g., pyridyl, pyrrolyl), nitro, cyano, haloalkyl, haloalkoxy, alkyl, alkoxy, cycloalkyl, heterocycloalkyl (e.g., pyrolidinyl, morpholin-4-yl, pyrrol-1-yl), cycloalkylalkyl, amino, alkylamino, dialkylamino, —OCF.sub.3, acyloxy, —OR.sup.8, —C(O)R.sup.9, —C(O)OR.sup.8, —NR.sup.10C(O)R.sup.9, —NR.sup.10C(O)OR.sup.8, —NR.sup.10S(O).sub.2R.sup.9, —S(O).sub.0-2R.sup.9 groups, carbonyl when two hydrogens attached to the same carbon atom of the cycloalkyl or heterocycloalkyl group of R′ are substituted, and ═CR.sup.8R.sup.9 when two hydrogens attached to the same carbon atom of the cycloalkyl or heterocycloalkyl groups of R.sup.1 are substituted, wherein each of the aryl, heteroaryl, cycloalkyl and heterocycloalkyl groups of the R.sup.3 and R.sup.4 moieties above is independently unsubstituted or substituted with 1 to 5 independently selected R.sup.12 moieties which can be the same or different, each R.sup.12 moiety being independently selected from the group consisting of: halo, phenyl, nitro, cyano, haloalkyl, haloalkoxy, alkyl, cycloalkyl, cycloalkylalkyl, amino, alkylamino, —OCF.sub.3, acyloxy, —OR.sup.8, —C(O)R.sup.9, —C(O)OR.sup.8, —NR.sup.10C(O)R.sup.9, —NR.sup.10C(O)OR.sup.8, —NR.sup.10S(O).sub.2R.sup.9, —S(O).sub.0-2R.sup.9 groups, carbonyl when two hydrogens attached to the same carbon atom of the cycloalkyl or heterocycloalkyl group of R.sup.3 or R.sup.4 are substituted, and ═CR.sup.8R.sup.9 when two hydrogens attached to the same carbon atom of the cycloalkyl or heterocycloalkyl group of R.sup.3 or R.sup.4 are substituted; or iii) R.sup.a and R.sup.b, together with the carbon to which they are both attached, form a 4- to 7-membered cycloalkyl or heterocycloalkyl ring, and R.sup.c and R.sup.d are each independently H or an alkyl group; or iv) R.sup.a and R.sup.c, together with the respective carbons to which they are attached, form a 4- to 7-membered cycloalkyl or heterocycloalkyl ring, and R.sup.b and R.sup.d are each independently H or an alkyl group, preferably R.sup.a and R.sup.c together have the cis configuration, e.g., where the carbons carrying R.sup.a and R.sup.c have the R and S configurations, respectively; v) R.sup.2 is H, halo, alkyl, haloalkyl, alkoxy, alkylthio, amino, aminosulfonyl, monoalkylamino, dialkylamino, hydroxyalkylamino, aminoalkylamino, carboxy, alkoxycarbonyl, aminocarbonyl or alkylaminocarbonyl group, wherein each alkyl group of R.sup.2 is independently unsubstituted or substituted with 1 to 5 independently selected R.sup.13 moieties which can be the same or different, each R.sup.13 moiety being independently selected from the group consisting of halo, hydroxy, alkoxy, alkyl, aryl (e.g., phenyl, naphthyl) heteroaryl (e.g., 1H-imidazol-2-yl), cycloalkyl, heterocycloalkyl (e.g., pyrolidin-1-yl), amino, monoalkylamino or dialkylamino group, wherein each aryl group of R.sup.13 is independently unsubstituted or substituted with 1 to 5 independently selected R.sup.4 moieties which can be the same or different; vi) Y is H or an alkyl group substituted with (i) an aryl, heteroaryl, cycloalkyl, hydroxy, alkoxy, amino, monoalkylamino or dialkylamino group, or (ii) an aryl group substituted with from one to three moieties each independently selected from the group consisting of: halo, alkyl, phenyl, hydroxy, alkoxy, phenoxy, amino, monoalkylamino and dialkylamino group; vii) each R.sup.8 is independently H, alkyl or aryl; viii) each R.sup.9 is independently H, alkyl, aryl or —NR.sup.10R.sup.11; ix) each R.sup.10 is independently H, alkyl, aryl, heteroaryl, arylalkyl or heteroarylalkyl, wherein each alkyl, aryl, heteroaryl, arylalkyl or heteroarylalkyl of R.sup.10 is unsubstituted or independently substituted with 1 to 5 R.sup.14 moieties which can be the same or different, each R.sup.14 moiety being independently selected from the group consisting of: halo, alkyl, aryl, cycloalkyl, —CF.sub.3, —OCF.sub.3, —CN, —OR.sup.8, —CH.sub.2OR.sup.8, —C(O)OR.sup.8 and —C(O)NR.sup.8R.sup.8; and x) each R.sup.11 is independently H, alkyl, aryl, heteroaryl, arylalkyl or heteroarylalkyl, wherein each alkyl, aryl, heteroaryl, arylalkyl or heteroarylalkyl of R.sup.11 is unsubstituted or independently substituted with 1 to 5 R.sup.14 moieties which can be the same or different.
(26) The invention further provides the use of PDE 1 Inhibitors of Formula VIIa or VIIb, in free or salt form, as follows: 4.1: Formula VIIa or VIIb, wherein q=0, 1 or 2; 4.2: Formula VIIa or VIIb, wherein q=0; 4.3: Formula VIIa or VIIb or 4.1 or 4.2, wherein R.sup.1 is alkyl; 4.4: Formula VIIa or VIIb or 4.1-4.2, wherein R.sup.1 is methyl; 4.5: Formula VIIa or VIIb or 4.1-4.4, wherein R.sup.a and R.sup.c, together with the respective carbons to which they are attached, form a 4- to 7-membered cycloalkyl or heterocycloalkyl ring, and R.sup.b and R.sup.d are each independently H or an alkyl group; 4.6: Formula VIIa or VIIb or 4.1-4.4, wherein R.sup.a and R.sup.c, together with the respective carbons to which they are attached, form a 5-membered heterocycloalkyl ring, and R.sup.b and R.sup.d are each independently H; 4.7: Formula VIIa or VIIb or 4.1-4.4, wherein R.sup.a and R.sup.b, together with the respective carbons to which they are attached, form a 5-membered heterocycloalkyl ring, and R.sup.c and R.sup.d are each independently H; 4.8: Formula VIIa or VIIb or 4.1-4.7, wherein R.sup.2 is alkyl or haloalkyl; 4.9: Formula VIIa or VIIb or 4.1-4.7, wherein R.sup.2 is biphenyl-4-ylmethyl; 4.10: Formula VIIa or VIIb or 4.1-4.7, wherein R.sup.2 is benzyl; 4.11: Formula VIIa or VIIb or 4.1-4.7, wherein R.sup.2 is cyclopentylmethyl; 4.12: Formula VIIa or VIIb or 4.1-4.7, wherein R.sup.2 is cyclopropylmethyl; 4.13: Formula VIIa or VIIb or 4.1-4.12, wherein Y is benzyl; and/or 4.14: Of any of the preceding formulae wherein the compound is selected from the following:
(27) ##STR00013## 4.15: Of any of the preceding formulae wherein the compound is
(28) ##STR00014## 4.16: A compound which is a substituted imidazo[2,1-b]purin-4-one, in free or pharmaceutically acceptable salt form, e.g. a compound of Formula VIIa or according to any of formulae 4.1-4.15, wherein the compound inhibits phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example 1 below.
(29) Preferably, compounds of Formula VIIa or VIIb are selected from a group consisting of (6aR,9aS)-5,6a,7,8,9,9a-hexahydro-5-methyl-2,3-bis(phenylmethyl)-yclopent[4,5]imidazo[2,1-b]purin-4(3H)-one, (6aR,9aS)-2-(biphenyl-4-ylmethyl)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylmethyl)cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one, 5′-methyl-2′,3′-bis(phenylmethyl)spiro[cyclopentane-1,7′(8′H)-[3H]imidazo[2,1-b]purin]-4′(5′H)-one and 5′-methyl-2′-(biphenyl-3′-(phenylmethyl)spiro-[cyclopentane-1,7′(8′H)-[3H]imidazo[2,1-b]purin]-4(5′H)-one, in free or pharmaceutically acceptable salt form.
(30) In an especially preferred embodiment, compound of Formula VIIa is (6aR,9aS)-2-(biphenyl-4-ylmethyl)-5,6a,7,8,9,9a-hexahydro-5-methyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one, in free or salt form.
(31) The numbering of substituted imidazo[2,1-b]purin-4-one of Formula VIIa or VIIb as described herein is shown below as an example, wherein q=0:
(32) ##STR00015##
wherein q=1:
(33) ##STR00016##
(34) In another embodiment, the PDE 1 Inhibitors for use in the methods of treatment described herein are Compounds of Formula VIIIa or VIIIb:
(35) ##STR00017##
in free or salt form, wherein: J is oxygen or sulfur, R.sup.1 is hydrogen, alkyl or alkyl substituted with aryl or hydroxy; R.sup.2 is hydrogen, aryl, heteroaryl, cycloalkyl, alkyl or alkyl substituted with aryl, heteroaryl, hydroxy, alkoxy, amino, monoalkyl amino or dialkylamino, or —(CH.sub.2).sub.m TCOR.sup.20 wherein m is an integer from 1 to 6, T is oxygen or —NH— and R.sup.20 is hydrogen, aryl, heteroaryl, alkyl or alkyl substituted with aryl or heteroaryl; R.sup.3 is hydrogen, halo, trifluoromethyl, alkoxy, alkylthio, alkyl, cycloalkyl, aryl, aminosulfonyl, amino, monoalkylamino, dialkylamino, hydroxyalkylamino, aminoalkylamino, carboxy, alkoxycarbonyl or aminocarbonyl or alkyl substituted with aryl, hydroxy, alkoxy, amino, monoalkylamino or dialkylamino; R.sup.a, R.sup.b, R.sup.c and R.sup.d independently represent hydrogen, alkyl, cycloalkyl or aryl; or (R.sup.a and R.sup.b) or (R.sup.c and R.sup.d) or (R.sup.b and R.sup.c) can complete a saturated ring of 5- to 7-carbon atoms, or (R.sup.a and R.sup.b) taken together and (R.sup.b and R.sup.c) taken together, each complete a saturated ring of 5- to 7-carbon atoms, wherein each ring optionally can contain a sulfur or oxygen atom and whose carbon atoms may be optionally substituted with one or more or the following: alkenyl, alkynyl, hydroxy, carboxy, alkoxycarbonyl, alkyl or alkyl substituted with hydroxy, carboxy or alkoxycarbonyl; or such saturated ring can have two adjacent carbon atoms which are shared with an adjoining aryl ring; and n is zero or one.
(36) The invention further provides the use of PDE 1 Inhibitors of Formula VIIIa or VIIIb, in free or salt form, as follows: 5.1: Formula VIIIa or VIIIb, wherein J=O. 5.2: Formula VIIIa or VIIIb or 5.1, wherein R.sup.1 is alkyl. 5.3: Formula VIIIa or VIIIb, 5.1 or 5.2, wherein R.sup.2 is hydrogen, benzyl, 4-chlorobenzyl, cyclohexylmethyl or trimethylacetoxymethyl. 5.4: Formula VIIIa or VIIIb, 5.1, 5.2 or 5.3, wherein R.sup.3 is hydrogen, or alkyl such as methyl or ethyl. 5.5: Formula VIIIa or VIIIb, 5.1, 5.2, 5.3 or 5.4, wherein n is zero; and 5.6: Formula VIIIa or VIIIb, 5.1, 5.2, 5.3, 5.4 or 5.5, wherein R.sup.a and R.sup.b form a saturated 5 membered ring, or (R.sup.b and R.sup.c) form a saturated 5, 6 or 7 membered ring, or (R.sup.a and R.sup.b) and (R.sup.b and R.sup.c) each complete a saturated ring and each ring contains 5 or 6 carbon atoms.
(37) The invention further provides the use of PDE 1 Inhibitors of Formula VIIIa or VIIIb, in free or salt form, selected from the following: cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-3-(phenylmethyl)cyclopenta[4,5]imidazo-[2,1-b]purin-4-one; 7,8-Dihydro-5-methyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; cis-6a,7,8,9,10,10a-Hexahydro-5-methyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)-one; 5,7,8,9-Tetrahydro-5-methyl-3-(phenylmethyl)pyrimido[2,1-b]purin-4(3H)-one; 7,8-Dihydro-8-phenyl-5-methyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 5′,7′-Dihydro-5′-methyl-3′-(phenylmethyl)spiro[cyclohexane-1,8′-(8H)imidazo-[2,1-b]purin]4′(3′H)-one; cis-5,6a,11,11a-Tetrahydro-5-methyl-3-(phenylmethyl)indeno[1′,2′:4,5]imidazo-[2,1-b]purin-4(3H)-one; 5′,7′-Dihydro-2′,5′dimethyl-3′-(phenylmethyl)spiro{cyclohexane-1,7′(8′H)-imidazo[2,1-b]purin}-4′-(3′H)-one; 7,8-Dihydro-2,5,7,7,8(R,S)-pentamethyl-3H-imidazo[2,1-b]purin-4(5H)-one; cis-5,6a,7,11b-Tetrahydro-5-methyl-3-(phenylmethyl)indeno[2′,1′,:4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2,5-dimethyl-3-(phenylmethyl)cyclopent[4,5]-imidazo[2,1-b]purin-4-(3H)-one; 5′-Methyl-3′-(phenylmethyl)-spiro[cyclopentane-1,7′-(8′H)-(3′H)imidazo[2,1-b]purin]-4-(5′H)-one; 7,8-Dihydro-2,5,7,7-tetramethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5′H)-one; 7,8-Dihydro-7(R)-phenyl-2,5-dimethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-3,7(R)-bis(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; (±)-7,8-Dihydro-2,5-dimethyl-7-ethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 6a(S)-7,8,9,10,10a(R)-Hexhydro-2,5-dimethyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)-one; 6a(R)-7,8,9,10,10a(S)-hexahydro-2,5-dimethyl-3-(phenylmethyl)-3H-benzimidazo-[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(R)-isopropyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5,7(R)-trimethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; cis-7,7a,8,9,10,10a-Hexahydro-2,5-dimethyl-3-(phenylmethyl)-3H-cyclopenta-[5,6]pyrimido[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(S)-(1-methylpropyl)-3-(phenylmethyl)-3H-imidazo-[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(R)-(2-methylpropyl)-3-(phenylmethyl)-3H-imidazo-[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(R,S)-(methoxycarbonyl)-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(R,S)-(1-propyl)-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(S)-(1-methylethyl)-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5,7,7,8(R,S)-pentamethyl-3H-imidazo[2,1-b]purin-4(5H)-one; 5,7,8,9-Tetrahydro-2,5,7,9(R,S)-pentamethyl-3-(phenylmethyl)-pyrimido[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(S),7,8,9,9a(R)-Hexahydro-2,5-dimethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-6a,7,8,9,10,10a-Hexahydro-2,5-dimethyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)-one; 5′,7′-Dihydro-2′,5′-dimethyl-3′-(phenylmethypspiro[cyclohexane-1,8-(8H)-imidazo[2,1-b]purin]-4-(3′H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2,5-dimethyl-3-(phenylmethypcyclohept-[6,7]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-2-ethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-6a,7,8,9,10,10a-Hexahydro-5-methyl-2-ethyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4-(5H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-2-ethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-2-phenyl-3-(phenylmethypcyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-6a,7,8,9,10,10a-Hexahydro-5-methyl-2-phenyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)-one; cis-5,6a,7,8.9,9a-Hexahydro-5-methylcyclopenta[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2,5-dimethylcyclopenta[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a(R), 7,8,9,9a(S)-Hexahydro-2,5-di-methylcyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; 2′,5′-dimethyl-spiro{cyclopentane-1,7′-(8′H)-(3′H)-imidazo[2,1-b]purin}-4′(5′H)-one; 7,8-Dihydro-2,5-dimethyl-7(R)-(1-methylethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5,7,7-tetramethyl-3H-imidazo[2,1-b]purin-4(5H)-one; 7,8-Dihydro-2,5-dimethyl-7(S)-(1-methylethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; 6a(R),7,8,9,10,10a(S)-Hexahydro-2,5-dimethyl-3H-benzimidazo[2,1-b]purin-4(5H)-one; 5′,7′-Dihydro-2′,5′-dimethylspiro{cyclohexane-1,7-(8′H)-imidazo[2,1-b]purin}-4′(3′H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-3-(phenylmethyl)cyclopenta[4,5]-imidazo[2,1-b]purin-4(3H)-thione; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-thione; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-3-(4-chlorophenylmethypcyclopenta[4,5]-imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-3-(cyclohexylmethyl)cyclopent[4,5]-imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-3-(2-naphthylmethyl)cyclopent[4,5]-imidazo[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-(4-bromophenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(R)-7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-(4-methoxyphenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2,3,5-trimethylcyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2-(hydroxymethyl)-5-methyl-3-(phenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2-methylthio-5-methyl-3-(Phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-3,4,5,6a,7,8,9,9a-Octahydro-5-methyl-4-oxo-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-2-carboxylic acid; cis-3,4,5,6a,7,8,9,9a-Octahydro-5-methyl-4-oxo-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-2-carboxylic acid, methyl ester; cis-5,6a,7,8,9,9a-Hexahydro-2-bromo-5-methyl-3-(phenylmethyl)cyclopent[4,5]imidazo[2,1-b]purin-4(3H)one; cis-5,6a,7,8,9,9a-Hexahydro-2-(methylaminosulfonyl)-5-methyl-3-(phenylmethyl)cyclopent[4,5]imidazo[2,1-b]purin-4(3H)one; cis-1-Cyclopentyl-5,6a,7,8,9,9a-hexahydro-5-methylcyclopent[4,5]imidazo[2,1-b]purin-4-(1H)one; cis-5,6a,7,8,9,9a-Hexahydro-3,5-bis-(phenylmethyl)cyclopent(4,5)imidazo(2,1-b)purin-4(3H)one; cis-6a,7,8,9,10,10a-Hexahydro-3,5-bis-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)one; cis-3-Cyclopentyl-5,6a,7,8,9,9a-hexahydro-5-methylcyclopent[4,5]imidazo(2,1-b)purin-4(3H)one; 5′-Methyl-3′-(phenylmethyl)spiro[cyclopentane-1,7-(8′H)-(3′H)imidazo[2,1-b]purin]-4-(5H)one; 2′,5′-Dimethyl-3′-(phenylmethyl)-spiro[cyclopentane-1,7-(8′H)-(3H)imidazo[2,1-b]purin]-4-(5′H)one; cis-5,6a,(R)7,8,9,9a(S)-Hexahydro-5-methyl-3-(phenylmethyl)cyclopent[4,5]-imidazo(2,1-b)purin-4(3H)one; cis-3-Cyclopentyl-5,6a,7,8,9,9a-Hexahydro-2,5-dimethylcyclopent[4,5]imidazo-[2,1-b]purin-4(3H)one; 5′-Methyl-2′-trifluoromethyl-3′-(phenylmethypspiro{cyclo-pentane-1,7′(8′H)-(3′H)imidazo[2,1-b]purin}-4-(5′H)-one; 7,8-Dihydro-5,7,7-trimethyl-2-trifluoromethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(5H)-one; (+/−)-cis-5,6a,7,8,9,9a-Hexahydro-5-methyl-2-trifluoromethyl-3-(phenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; (+/−)-6a,7,8,9,9a,10,11,11 a-Octahydro-2,5-dimethyl-3-(phenylmethyl)-3H-pentaleno[6a′,1′:4,5]imidazo[2,1-b]purin-4(5H)-one; (+)-6a,7,8,9,9a,10,11,11a-Octahydro-2,5-dimethyl-3-phenylmethyl-3H-pentaleno[6a′,1′:4,5]imidazo[2,1-b]purin-4(5H)-one; (−)-6a,7,8,9,9a,10,11,11a-Octahydro-2,5-dimethyl-3-phenylmethyl-3H-pentaleno[6a′,1′:4,5]Imidazo[2,1-b]purin-4(5H)-one; (+/−) 6a,7,8,9,9a,10,11,11a-Octahydro-2,5-dimethyl-3H-pentaleno[6a′,1′:4,5]-imidazo[2,1-b]purin-4(5H)-one; (+)-6a,7,8,9,9a,10,11,11a-Octahydro-2,5-dimethyl-3H-pentaleno[6a′,1′:4,5]-imidazo[2,1-b]purin-4(5H)-one; (−)-6a,7,8,9,9a,10,11,11a-Octahydro-2,5-dimethyl-3H-pentaleno[6a′,1′:4,5]-imidazo[2,1-b]purin-4(5H)-one; 6a,7,8,9,10,10a,11,12,13,13a-Decahydro-2,5-dimethyl-(3-phenylmethyl)-napth[1,8a-d]imidazo[2,1-b]purin-4(5H)one; 7(R)-Cyclohexyl-7,8-dihydro-2,5-dimethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(3H)-one; 7(R)-Cyclohexyl-7,8-dihydro-2,5-dimethyl-3H-imidazo[2,1-b]purin-4(5H)-one; 7(S)-Cyclohexyl-7,8-dihydro-2,5-dimethyl-3-(phenylmethyl)-3H-imidazo[2,1-b]purin-4(3H)-one; 7(S)-Cyclohexyl-7,8-dihydro-2,5-dimethyl-3H-imidazo[2,1-b]purin-4(5H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-[3-(trimethylacetoxy)methyl]-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-(4-pyridylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-[2-(4-morpholinyl)-ethyl]cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5-dimethyl-3-[acetoxymethyl]cyclopent-[4,5]imidazo[2.1-b]purin-4(3H)-one; 5,6a,7,8,9,9a-Hexahydro-2,5,6a-trimethyl-3-(phenylmethyl)cyclopent-[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(R),7,8,9,9a(S)-Hexahydro-2,5,6a-trimethyl-3-(phenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; 5,6a(S),7,8,9,9a(R)-Hexahydro-2,5,6a-trimethyl-3-(phenylmethyl)-cyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; cis-6a,7,8,9,10,10a-Hexahydro-2,5,7-trimethyl-3-(phenylmethyl)-3H-benzimidazo[2,1-b]purin-4(5H)-one; cis-5,6a,7,8,9,9a-Hexahydro-2,5,6a-trimethylcyclopent[4,5]imidazo[2,1-b]purin-4(3H)-one; or cis-[6a,7,8,9,10,10a-Hexahydro-2,5,7-trimethyl-3H-benzimidazo[2,1-b]purin-4(5H)-one].
(38) In another embodiment, the PDE 1 Inhibitors for use in the methods of treatment described herein are Compounds of Formula IXa or IXb:
(39) ##STR00018## or a pharmaceutically acceptable salt thereof, wherein, q=0 or 1; R.sup.1 is H, cycloalkyl, alkyl, R.sup.23-alkyl- or R.sup.26; R.sup.a, R.sup.b and R.sup.c are, independently of one another, each H, alkyl, cyoloalkyl, aryl, R.sup.22-aryl- or R.sup.24-alkyl-; or R.sup.a and R.sup.b, together with the carbon to which they are both attached, form a 4- to 7-membered ring, and R.sup.c is H or alkyl; or R.sup.a and R.sup.c, together with the respective carbons to which they are attached, form a 4- to 7-membered ring, and R.sup.b is H or alkyl; (i) X is a bond; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is monohaloalkyl, polyhaloalkyl, provided that it is not trifluoromethyl, azido, cyano, oximino, cycloalkenyl, heteroaryl, R.sup.22-heteroaryl- or R.sup.27-alkyl-; (ii) X is a bond; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is H, halo, —CONHR.sup.6, —CONR.sup.6R.sup.7, —CO.sub.2R.sup.6, monohaloalkyl, polyhaloalkyl, azido, cyano, —C═N—OR.sup.6, cycloalkyl, cycloalkylalkyl, R.sup.26, aminosulfonyl, alkyl or R.sup.23-alkyl- (iii) X is —O— or —S—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is R.sup.26, cycloalkyl cycloalkylalkyl, heterocycloalkyl, cycloalkenyl or R.sup.26-alkyl-; (iv) X is —O— or —S—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is alkyl, R.sup.26, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, cycloalkenyl or R.sup.28-alkyl-; (v) X is —SO— or —SO.sub.2—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is alkyl, R.sup.26, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, cycloalkenyl or R.sup.28-alkyl-; (vi) X is —NR.sup.8—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is (R.sup.29).sub.p-alkyl-, cycloalkyl, (R.sup.30).sub.p-cycloalkyl-, cycloalkenyl, (R.sup.39).sub.p— cycloalkenyl-, heterocycloalkyl or (R.sup.30).sub.p-heterocycloalkyl-: (vii) X is —NR.sup.8—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is alkyl, R.sup.26, cycloalkyl, cycloalkylalkyl, heterocycloalkyl, cycloalkenyl or R.sup.31-alkyl-; or (viii) X is —C≡C—; Y is aryl-alkyl or R.sup.22-aryl-alkyl-; and R.sup.2 is alkyl, R.sup.26, cycloalkyl, cycloalkylalkyl or R.sup.23-alkyl-;
where, R.sup.6 is H or R.sup.7; R.sup.7 is alkyl, cycloalkyl or cycloalkylalkyl; R.sup.8 is heterocycloalkyl or R.sup.6; R.sup.21 is 1-6 substituents each independently selected from the group consisting of halo, hydroxy, alkoxy, phenoxy, phenyl, nitro, aminosulfonyl, cyano, monohaloalkyl, polyhaloalkyl, thiol, alkylthio, cyoloalkyl, cycloalkylalkyl, amino, alkylamino, acylamino, carboxyl, —C(O)OR.sup.34, carboxamido, —OCF.sub.3 and acyloxy; R.sup.22 is 1-6 substituents each independently selected from the group consisting of alkyl and R.sup.21; R.sup.23 is cycloalkoxy aryloxy, alkylthio, arylthio, cycloalkyl or R.sup.28; R.sup.24 is cycloalkyl or R.sup.26; R.sup.25 is hydroxy, alkoxy, amino, monoalkylamino, dialkylamino or R.sup.26; R.sup.26 is aryl, R.sup.22-aryl-, heteroaryl or R.sup.22-heteroaryl-; R.sup.27 is cycloalkoxy, aryloxy, alkylthio, arylthio, heteroaryl, R.sup.22-heteroaryl-, cycloalkyl, heterocycloalkyl, cycloalkenyl, cycloalkylamino or heterocycloalkylamino; R.sup.28 is cycloalkylamino, heterocycloalkylamino or R.sup.25; R.sup.29 is alkoxy, cycloalkylamino, heterocycloalkylamino or R.sup.26; R.sup.30 is halo, hydroxy, alkoxy, amino, aminosulfonyl, cyano, monohaloalkyl, polyhaloalkyl, thiol, alkylthio, alkyl, cyoloalkyl, cycloalkylalkyl or acyloxy; R.sup.31 is cycloalkyl or R.sup.28; R.sup.34 is alkyl, aryl, aralkyl and heteroaryl; and p is 1 to 4. 6.1 The invention further provides the use of PDE 1 Inhibitors of Formula IXa or IXb, in free or salt form, selected from the following:
(40) ##STR00019## ##STR00020## ##STR00021## ##STR00022## ##STR00023## ##STR00024## ##STR00025## ##STR00026##
(41) In another embodiment, the invention provides the use of PDE 1 Inhibitors of Formula X:
(42) ##STR00027## in free or a pharmaceutically acceptable salt thereof, wherein: R.sub.1, R.sub.2 and R.sub.3 are independently selected from the group consisting of hydrogen, lower alkyl, lower alkoxy, halogeno, hydroxy, (di-lower alkyl)amino, 4-morpholinyl, 1-pyrrolidinyl, 1-pyrrolyl, —CF.sub.3, —OCF.sub.3, phenyl and methoxyphenyl; or R.sub.1 and R.sub.2 together are methylenedioxy; or R.sub.1 and R.sub.2 together with the carbon atoms to which they are attached form a benzene ring; and R.sup.a is hydrogen and R.sup.b and R.sup.c, together with the carbon atoms to which they are attached, form a saturated ring of 5 carbons; or R.sup.a is lower alkyl, R.sup.b is hydrogen or lower alkyl, and R.sup.c is hydrogen; or R.sup.a, R.sup.b and the carbon atom to which they are attached form a saturated ring of 5-7 carbons, and R.sup.c is hydrogen; or R.sup.a is hydrogen, and R.sup.b, R.sup.c and the carbon atoms to which they are attached form a tetrahydrofuran ring; or R.sup.a and R.sup.b, together with the carbon atom to which they are attached, and R.sup.b and R.sup.c, together with the carbon atoms to which they are attached, each form a saturated ring of 5-7 carbons.
(43) In a further embodiment, the invention provides the use of PDE 1 Inhibitors of Formula X as follows: 7.1 Formula X, wherein R.sub.1, R.sub.2 and R.sub.3 are independently selected from the group consisting of hydrogen, lower alkyl, lower alkoxy, halogeno, hydroxy, (di-lower alkyl)amino, 4-morpholinyl, 1-pyrrolidinyl, 1-pyrrolyl, —CF.sub.3, —OCF.sub.3, phenyl and methoxyphenyl; or R.sub.1 and R.sub.2 together are methylenedioxy; or R.sub.1 and R.sub.2 together with the carbon atoms to which they are attached form a benzene ring; 7.2 Formula X or 7.1, wherein R.sub.1 is H, methoxy or trifluoromethyl; 7.3 Formula X or 7.1 or 7.2, wherein R.sub.1 is H; 7.4 Formula X or any of 7.1-7.3, wherein R.sub.2 is selected from a group consisting of H, halo (e.g., F, Cl), methoxy, methyl, trifluoromethyl, dimethylamino, phenyl, methoxyphenyl-, —OCF.sub.3, 3,4-OCH.sub.2O—, pyrolidin-1-yl, pyrol-1-yl and morpholin-4-yl; 7.5 Formula X or any of 7.1-7.4, wherein R.sub.1 and R.sub.2 together with the carbon atoms to which they are attached forma a benzene ring; 7.6 Formula X or any of 7.1-7.5, wherein R.sub.3 is H or methoxy; 7.7 Formula X or any of 7.1-7.6, wherein R.sub.3 is H; 7.8 Formula X or any of 7.1-7.7, wherein R.sup.a is hydrogen and R.sup.b and R.sup.c, together with the carbon atoms to which they are attached, form a saturated ring of 5 carbons; or R.sup.a is lower alkyl, R.sup.b is hydrogen or lower alkyl, and R.sup.c is hydrogen; or R.sup.a, R.sup.b and the carbon atom to which they are attached form a saturated ring of 5-7 carbons, and R.sup.c is hydrogen; or R.sup.a is hydrogen, and R.sup.b, R.sup.c and the carbon atoms to which they are attached form a tetrahydrofuran ring; or R.sup.a and R.sup.b, together with the carbon atom to which they are attached, and R.sup.b and R.sup.c, together with the carbon atoms to which they are attached, each form a saturated ring of 5-7 carbons; 7.9 Formula X or any of 7.1-7.8, wherein R.sup.a is hydrogen and R.sup.b and R.sup.c together with the carbon atoms to which they are attached, form a saturated ring of 5 carbons, and wherein R.sub.1, R.sub.2 and R.sub.3 are as defined in the following table
(44) TABLE-US-00001 R.sub.1 R.sub.2 R.sub.3 H H H —OCH.sub.3 H H H F H H —OCH.sub.3 H H OH H H —CH.sub.3 H H (CH.sub.3).sub.2N— H —OCH.sub.3 —OCH.sub.3 —OCH.sub.3 —OCH.sub.3 —OCH.sub.3 H —CF.sub.3 H H H C.sub.2H.sub.5— H H —OCF.sub.3 H H
(45) ##STR00032## ##STR00033## 7.11 Formula X or any of 7.1-7.9, selected from a group consisting of: 2′-benzyl-5′-methyl-spiro[cyclopentane-1′,7′(8′H)-[3′H]-imidazo[2,1-b]purin]-4′-(5′H)-one; 2′-benzyl-5,7,7-trimethyl-3H-imidazo[2,1-b]purin-4-(5H)-one; (+)-2-benzyl-7,8-dihydro-5-methyl-7-(1-methylethyl)-1H-imidazo[2,1-b]-purin-4(5H)-one; (+,−)-6a,7,8,9,9a,10,11,11a-octahydro-5-methyl-2-(3,4-methylene-dioxyphenylmethyl)-3H-pentalen[6a, 1:4,5]imidazo[2,1-b]purin-4(5H)-one; and (+)-cis-6a,7,9,9a-tetrahydro-5-methyl-2-[4-(trifluoromethyl)-phenylmethyl]-3H-furo[3′,4′:4,5]imidazo[2,1-b]purin-4(5H)-one, in free or salt form. 7.12 Formulae X or 7.1-7.11, wherein the compounds inhibit phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example 1;
(46) In another embodiment, the invention provides the use of PDE 1 Inhibitors selected from the following:
(47) ##STR00034##
in free or salt form (Formula XI).
(48) The invention also provides novel 2-(optionally hetero)arylmethyl-3-(optionally hetero)arylamino-[2H]-pyrazolo[3,4-d]pyrimidine-4,6(5H,7H)-diones, in free, salt or prodrug form (hereinafter “Compounds of the Invention”). The (optionally)hetero aryl moiety at the 2-position is preferably benzyl or pyridyl methyl para-substituted relative to the point of attachment with aryl or heteroaryl, e.g., substituted with phenyl, pyridyl or thiadiazolyl. These compounds are surprisingly found to selectively inhibit phosphodiesterase 1 (PDE1) activity, e.g., PDE1A, PDE1B, and PDE1C activity, especially PDE1B activity.
(49) Preferably, the Compounds of the Invention are pyrazolo[3,4-d]pyrimidine-4,6(5H,7H)-diones of formula XII
(50) ##STR00035##
(51) Formula XII
(52) wherein
(53) (i) R.sub.1 is H or alkyl (e.g., methyl);
(54) (ii) R.sub.2 is H, alkyl (e.g., isobutyl, 2-methylbutyl, 2,2-dimethyl propyl), cycloalkyl (e.g., cyclopentyl, cyclohexyl), haloalkyl (e.g., trifluoromethyl, 2,2,2-trifluoroethyl), alkylaminoalkyl (e.g., 2-(dimethylamino)ethyl), hydroxyalkyl (e.g., 3-hydroxy-2-methyl propyl), arylalkyl (e.g., benzyl), heteroarylalkyl (e.g., pyridylmethyl), or alkoxyarylalkyl (e.g., 4-methoxybenzyl);
(55) (iii) R.sub.3 is a substituted heteroarylaklyl, e.g., substituted with haloalkyl
(56) or
(57) R.sub.3 is attached to one of the nitrogens on the pyrazolo portion of Formula XII and is
(58) a moiety of Formula C
(59) ##STR00036##
(60) wherein X, Y and Z are, independently, N or C, and R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are independently H or halogen (e.g., Cl or F); and R.sub.10 is halogen, alkyl, cycloalkyl, haloalkyl (e.g., trifluoromethyl), aryl (e.g., phenyl), heteroaryl (e.g., pyridyl, (for example, pyrid-2-yl) or e.g., thiadiazolyl (for example, 1,2,3-thiadiazol-4-yl), diazolyl, triazolyl (e.g., 1,2,4-triazol-1-yl), tetrazolyl (e.g., tetrazol-5-yl), alkoxadiazolyl (e.g., 5-methyl-1,2,4-oxadiazol), pyrazolyl (e.g., pyrazol-1-yl), alkyl sulfonyl (e.g., methyl sulfonyl), arylcarbonyl (e.g., benzoyl), or heteroarylcarbonyl, alkoxycarbonyl, (e.g., methoxycarbonyl), aminocarbonyl; preferably phenyl or pyridyl, e.g., 2-pyridyl; provided that when X, Y or X is nitrogen, R.sub.8, R.sub.9 or R.sub.10, respectively, is not present;
(61) (iv) R.sub.4 is aryl (e.g., phenyl) or heteroaryl; and
(62) (v) R.sub.5 is H, alkyl, cycloalkyl (e.g., cyclopentyl), heteroaryl, aryl, p-benzylaryl (e.g., biphenyl-4-ylmethyl);
(63) wherein “alk” or “alkyl” refers to C.sub.1-6 alkyl and “cycloalkyl” refers to C.sub.3-6 cycloalkyl; in free, salt or prodrug form
(64) The invention further provides compounds of Formula XII as follows:
(65) 8.1 Formula XII wherein R.sub.1 is methyl;
(66) 8.2 Formula XII or 12.1 wherein R.sub.2 is C.sub.1-6 alkyl;
(67) 8.3 Formula 12.2 wherein R.sub.2 is isobutyl, 2,2-dimethyl propyl, or 2-methylbutyl;
(68) 8.4 Formula XII or 12.1 wherein R.sub.2 is hydroxy C.sub.1-6 alkyl;
(69) 8.5 Formula XII or 12.1 wherein R.sub.2 is 3-hydroxy-2-methyl propyl;
(70) 8.6 Formula XII or 12.1 wherein R.sub.2 is C.sub.1-6 alkoxy-benzyl;
(71) 8.7 Formula 12.6 wherein R.sub.2 is p-methoxybenzyl;
(72) 8.8 Formula XII or 12.1 wherein R.sub.2 is C.sub.3-6 cycloalkyl;
(73) 8.9 Formula 12.8 wherein R.sub.2 is cyclopentyl or cyclohexyl;
(74) 8.10 Formula XII or 12.1 wherein R.sub.2 is C.sub.1-6 haloalkyl;
(75) 8.11 Formula 12.10 wherein R.sub.2 is 2,2,2-trifluoroethyl;
(76) 8.12 Any of the preceding formulae wherein R.sub.3 is a moiety of Formula A wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are each H and R.sub.10 is phenyl;
(77) 8.13 Any of the preceding formulae XII-12.11 wherein R.sub.3 is a moiety of Formula A wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are each H and R.sub.10 is pyridyl or thiadizolyl;
(78) 8.14 Formula 12.13 wherein R.sub.3 is a moiety of Formula A wherein R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are each H and R.sub.10 is 2-pyridyl;
(79) 8.15 Any of the preceding formulae wherein R.sub.4 is phenyl;
(80) 8.16 Any of the preceding formulae wherein R.sub.5 is H;
(81) 8.17 Any of the preceding formulae wherein X, Y and Z are all C;
(82) 8.18 Any of the preceding formulae wherein R.sub.2 is tetrahydrofuran-2-ylmethyl;
(83) 8.19 Any of the preceding formulae wherein R.sub.10 is pyrimidinyl;
(84) 8.20 A compound of formula 12.19 wherein the pyrimidinyl is 5-fluoropyrmidinyl;
(85) 8.21 Any of the preceding formulae wherein R.sub.10 is pyrazol-1-yl;
(86) 8.22 Any of the preceding formulae wherein R.sub.10 is 1,2,4-triazol-1-yl;
(87) 8.23 Any of the preceding formulae wherein R.sub.10 is aminocarbonyl;
(88) 8.24 Any of the preceding formulae wherein R.sub.10 is methylsulfonyl;
(89) 8.25 Any of the preceding formulae wherein R.sub.10 is 5-methyl-1,2,4-oxadiazol-3-yl;
(90) 8.26 Any of the preceding formulae wherein R.sub.10 is 5-fluoropyrimidin-2-yl;
(91) 8.26 Any of the preceding formulae wherein R.sub.4 is 4-fluorophenyl;
(92) 8.27 Any of the preceding formulae wherein R.sub.10 is trifluoromethyl;
(93) 8.28 Any of the preceding formulae wherein R.sub.3 is a moiety of Formula C, X and Z are C, and Y is N;
(94) 8.29 A compound selected from the compounds of Examples 1-24 below; and/or
(95) 8.30 Any one of the preceding formulae wherein the compounds inhibit phosphodiesterase-mediated (e.g., PDE1-mediated, especially PDE1B-mediated) hydrolysis of cGMP, e.g., with an IC.sub.50 of less than 1 μM, preferably less than 25 nM in an immobilized-metal affinity particle reagent PDE assay, for example, as described in Example I; such compounds according to any of the preceding formulae being in free, salt or prodrug form.
(96) In an especially preferred embodiment, the Compounds of the Invention are compounds of Formula XII wherein
(97) (i) R.sub.1 is methyl;
(98) (ii) R.sub.2 is C.sub.1-6 alkyl;
(99) (iii) R.sub.3 is a moiety of Formula C wherein X, Y and Z are all C and R.sub.8, R.sub.9, R.sub.11 and R.sub.12 are each H and R.sub.10 is phenyl, pyridyl (for example, pyrid-2-yl), or thiadiazolyl (e.g., 1,2,3-thiadiazol-4-yl);
(100) (iv) R.sub.4 is phenyl; and
(101) (v) R.sub.5 is H; in free or salt form.
(102) For example, the methods of treatment include compounds according to Formula XIII:
(103) ##STR00037##
(104) wherein R.sub.2 is H, alkyl (e.g., isobutyl, 2-methylbutyl, 2,2-dimethyl propyl), cycloalkyl (e.g., cyclopentyl, cyclohexyl), heteroaryl (e.g., pyridyl), aryl (e.g., phenyl), haloalkyl (e.g., trifluoromethyl, 2,2,2-trifluoroethyl), alkylaminoalkyl (e.g., 2-(dimethylamino)ethyl), hydroxyalkyl (e.g., 3-hydroxy-2-methyl propyl), arylalkyl (e.g., benzyl), or alkoxyarylalkyl (e.g., 4-methoxybenzyl);
(105) wherein “alk” or “alkyl” refers to C.sub.1-6 alkyl; and
(106) R.sub.10 is phenyl, pyridyl (for example, pyrid-2-yl) or thiadiazolyl (for example,
(107) 1,2,3-thiadiazol-4-yl);
(108) in free, salt or prodrug form
(109) In certain embodiments, the Compounds of the Invention are compounds of Formula XIII wherein
(110) R.sub.2 is H, alkyl (e.g., isobutyl, 2-methylbutyl, 2,2-dimethyl propyl), cycloalkyl (e.g., cyclopentyl, cyclohexyl, tetrahydrofuran-2-ylmethyl), heteroaryl (e.g., pyridyl), aryl (e.g., phenyl), haloalkyl (e.g., trifluoromethyl, 2,2,2-trifluoroethyl), alkylaminoalkyl (e.g., 2-(dimethylamino)ethyl), hydroxyalkyl (e.g., 3-hydroxy-2-methyl propyl), arylalkyl (e.g., benzyl), or alkoxyarylalkyl (e.g., 4-methoxybenzyl);
(111) and R.sub.10 is phenyl, pyridyl (for example, pyrid-2-yl), pyrimidinyl (e.g., 5-fluoropyrimidin-2-yl), pyrazolyl (e.g. pyrazol-1-yl), thiadiazolyl (for example, 1,2,3-thiadiazol-4-yl), haloalkyl (e.g., trifluoromethyl), alkylsulfonyl (e.g., methylsulfonyl), oxadiazolyl (e.g., 5-methyl-1,2,4-oxadiazol-3-yl), aminocarbonyl (e.g., so as to form a 4-benzamide structure), triazolyl (e.g., 1,2,4-triazol-1-yl);
(112) wherein “alk” or “alkyl” refers to Ci_.sub.6 alkyl;
(113) in free, salt or prodrug form.
(114) If not otherwise specified or clear from context, the following terms as used herein have the following meetings: a. “Alkyl” as used herein is a saturated or unsaturated hydrocarbon moiety, preferably saturated, preferably one to seven carbon atoms in length, which may be linear or branched, and may be optionally substituted, e.g., mono-, di-, or tri-substituted, e.g., with halogen (e.g., chloro or fluoro), hydroxy, or carboxy. b. “Cycloalkyl” as used herein is a saturated or unsaturated nonaromatic hydrocarbon moiety, preferably saturated, preferably comprising three to nine carbon atoms, at least some of which form a nonaromatic mono- or bicyclic, or bridged cyclic structure, and which may be optionally substituted, e.g., with halogen (e.g., chloro or fluoro), hydroxy, or carboxy. c. “Heterocycloalkyl” as used herein is a saturated or unsaturated nonaromatic hydrocarbon moiety, preferably saturated, preferably comprising three to nine carbon atoms, at least one atom selected from a group consisting of N, O or S, at least some of which form a nonaromatic mono- or bicyclic, or bridged cyclic structure, and which may be optionally substituted, e.g., with halogen (e.g., chloro or fluoro), hydroxy, or carboxy. Examples of heterocycloalkyl include pyrrolidinyl (e.g., pyrrolidin-1-yl), morpholinyl (e.g., morpholin-4-yl), d. “Aryl” as used herein is a mono or bicyclic aromatic hydrocarbon (e.g., phenyl, naphthyl), preferably phenyl, optionally substituted, e.g., with alkyl (e.g., methyl), halogen (e.g., chloro or fluoro), haloalkyl (e.g., trifluoromethyl), hydroxy, carboxy, or an additional aryl or heteroaryl (e.g., biphenyl or pyridylphenyl). e. “Heteroaryl” as used herein is an aromatic moiety wherein one or more of the atoms making up the aromatic ring is sulfur or nitrogen rather than carbon, e.g., pyridyl, thiadiazolyl, pyrrolyl (e.g., pyrrol-2-yl) or imidazolyl (e.g., 1H-imidazol-2-yl), which may be optionally substituted, e.g., with alkyl, halogen, haloalkyl, hydroxy or carboxy.
(115) PDE 1 Inhibitors may exist in free or salt form, e.g., as acid addition salts. In this specification unless otherwise indicated language such as PDE 1 Inhibitors is to be understood as embracing the compounds in any form, for example free or acid addition salt form, or where the compounds contain acidic substituents, in base addition salt form. The PDE 1 Inhibitors are intended for use as pharmaceuticals, therefore pharmaceutically acceptable salts are preferred. Salts which are unsuitable for pharmaceutical uses may be useful, for example, for the isolation or purification of free PDE 1 Inhibitors or their pharmaceutically acceptable salts.
(116) PDE 1 Inhibitors may in some cases also exist in prodrug form, for example when the compounds contain physiologically hydrolysable and acceptable esters. As used herein, “physiologically hydrolysable and acceptable ester” means esters of PDE 1 Inhibitors which are hydrolysable under physiological conditions to yield acids (in the case of PDE 1 Inhibitors which have hydroxy substituents) or alcohols (in the case of PDE 1 Inhibitors which have carboxy substituents) which are themselves physiologically tolerable at doses to be administered. As will be appreciated the term thus embraces conventional pharmaceutical prodrug forms.
(117) The pharmaceutical composition, which includes a PDE 1 inhibitor and optionally, an intraocular pressure lowering agent, may be formulated according to known methods for preparing pharmaceutically useful compositions. The pharmaceutically acceptable carrier can include diluents, adjuvants, and vehicles, as well as implant carriers, and inert, non-toxic solid or liquid fillers, diluents, or encapsulating material that does not react with the active ingredients used in the compositions. Examples include, but are not limited to, phosphate buffered saline, physiological saline, water, and emulsions, such as oil/water emulsions.
(118) The pharmaceutical carrier can be a solvent or dispersing medium containing, for example, ethanol, polyol (for example, glycerol, propylene glycol, liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetable oils. Formulations containing pharmaceutically acceptable carriers are described in a number of sources which are well known and readily available to those skilled in the art. For example, Remington's The Science and Practice of Pharmacy, 21.sup.st edition, describes formulations that can be used in connection with the compositions.
(119) Methods of making and formulating the PDE 1 Inhibitors, novel intermediates useful for making PDE 1 Inhibitors, and methods of using the PDE 1 Inhibitors for treatment of diseases are generally disclosed in EP 0201188 (or U.S. Pat. No. 4,666,908) and EP 0911333 (or U.S. Pat. No. 6,235,742); PCT/US2006/022066; PCT/US2006/033179; WO 03/042216 (U.S. Pat. No. 6,943,171); U.S. Pat. Nos. 6,969,719; 5,939,419; EP 0 538 332 (U.S. Pat. No. 5,393,755); Xia et al., J. Med. Chem. (1997), 40, 4372-4377 and Ahn et al., J. Med. Chem. (1997), 40, 2196-2210, the contents of each of which are incorporated herein by reference by their entirety.
(120) In another example, an ophthalmic composition which includes ophthalmic solutions, may be prepared using at least one of the PDE-1 inhibitors or a combination. Procedures for preparing ophthalmic compostions are described in Remington's: The Science and Practice of Pharmacy, 21.sup.st ed., see pgs. 856-863, for example.
(121) In one example, the effective amount of the PDE 1 inhibitor in the ophthalmic solution is a subthreshold amount.
(122) The ophthalmic composition can additionally include an agent known to lower intraocular pressure, preferably a subthreshold effective amount of the additional agent.
(123) The agent may be an adrenergic agonist, a beta-adrenergic antagonist, a prostaglandin or prostaglandin analog or a muscarinic analog, or an agent that raises cyclic nucleotides, a prostanoid, bimatoprost, brimonidine tartrate, or brimonidine tartrate/timolol maleate, or a combination. In addition agnonists selective for a particular receptor subtype may be used, such as alpha-2 adrenergic receptor agonist, for example. Other agents known to treat glaucoma may be used.
(124) The ophthalmic composition may be prepared in the form of a gel-forming solution, a semi-solid aqueous gel or other ophthalmic gels, an ophthalmic suspension ophthalmic ointment or an ophthalmic emulsion, or an ophthalmic solution,
(125) Other forms of ophthalmic delivery include a viscoelastic solution such as a solution containing a highly purified fraction of sodium hyaluronate, chrondroitin sulfate, or purified hydroxypropyl methylcellulose, for example.
(126) Other examples of preparing ophthalmic compositions known to a person of ordinary skill in the art may be utilized.
(127) However, other means of drug administrations are well within the scope of the composition. Systemic therapeutic means may also be utilized.
(128) The PDE-1 inhibitors disclosed in this application may be combined with a subthreshold effective amount of glaucoma drugs by Allergan.
(129) In one example, a subthreshold effective amount of a PDE-1 inhibitor and a subthreshold amount of bimatoprost ophthalmic solution, marketed as LUMIGAN® is administered to a patient in need thereof, for treatment or management of glaucoma.
(130) In one example, a subthreshold effective amount of a PDE-1 inhibitor and a subthreshold amount of brimonidine tartrate ophthalmic solution, marketed as ALPHAGAN® is administered to a patient in need thereof, for treatment or management of glaucoma.
(131) In one example, a subthreshold effective amount of a PDE-1 inhibitor and a subthreshold amount of briimonidine tartrate/timolol ophthalmic solution, marketed as COMBIGAN® is administered to a patient in need thereof, for treatment or management of glaucoma.
(132) In another example, a subthreshold effective amount of a PDE-1 inhibitor and a subthreshold amount of a combination of LUMIGAN®, ALPHAGAN®, and COMBIGAN®, may be administered together.
(133) In another example, an effective amount of an ophthalmic prostaglandin, or a prostaglandin analog or a combination of the prostaglandin and the prostaglandin analog may be combined with an effective amount of the PDE-1 inhibitor to treat glaucoma, elevated intraocular pressure, or symptoms of glaucoma. The effective amount is interpreted to include a subthreshold amount. In one example, a subthreshold amount of a prostaglandin or a prostaglandin analog or a combination may be combined with a subthreshold amount of a PDE-1 inhibitor, to treat glaucoma, or elevated intraocular pressure. Examples of prostaglandins and prostaglandin analogs include travoprost, latanoprost, bimatroprost, an active ingredient in LUMIGAN,® unoprostone, and unoprostone isopropyl, an active ingredient in RESCULA.® There have been reports in the literature that many of the prostaglandins and their analogs such as bimatroprost and latanoprost have been associated with side effects that include increased eyelash growth and browning of the iris. Other side effects also include eye redness and itchy eyes. Thus, a subthreshold amount of a prostaglandin or an analog or a combination may be combined with an effective dosage of the PDE-1 inhibitor, to reduce or treat glaucoma, or elevated intraocular pressure, without the associated side effects.
(134) In another embodiment, the disclosed PDE 1 inhibitors, either alone, or in combination with other therapeutic agents, may be used for the treatment of diabetic retinopathy, a disease caused by complications of diabetes mellitus. Conventional treatments include laser surgery, injection of triamcinolone and vitrectomy. However, these treatments do not cure diabetic retinopathy and often have complications. For example, laser surgery may cause loss of retinal tissue. Triamcinolone may cause a marked increase in vision. Vitrectomy replaces the cloudy vitreous solution and replaces it with saline; however, surgical intervention may not be helpful in all patients. Thus, the disclosed PDE 1 inhibitors, either alone or in combination with other therapeutic agents, such as those disclosed here, may an alternative for the treatment of diabetic retinopathy.
(135) In another embodiment, the PDE 1 Inhibitors are compounds of Formula Ia selected from the following:
(136) ##STR00038##
(137) Alternatively, an effective amount of a PDE-1 inhibitor may be synergistically combined with an effective amount of a prostaglandin or a prostaglandin analog for effectively increasing eyelash growth.
(138) Methods of Treatment
(139) The invention provides methods of treatment in a human or animal patient suffering from glaucoma or elevated intraocular pressure or intraocular hypertension that may be ameliorated by said enhancement comprising administering an effective amount of a PDE 1 inhibitor, e.g., a PDE 1 Inhibitor as hereinbefore described, for example a Compound of Formula I, Ia, II, III, IV, V, VIIa, VIIb, VIIIa, VIIIb, IXa, IXb, or any of Formulae 1.2-1.17, 2.1-2.9, 3.2-3.22, 4.1-4.16, 5.1-5.6 to a human or animal patient, preferably a human, in need thereof. PDE 1 inhibitors of said method also include Compound of Formula X or XI or any of 6.1 or 7.1-7.12.
(140) PDE 1 Inhibitors may be used in the foregoing methods of treatment or prophylaxis as a sole therapeutic agent, but may also be used in combination or for co-administration with other active agents, for example in conjunction with other intraocular pressure lowering agents. Thus, the invention further comprises a method of treating disorders that may be ameliorated by enhancement of progesterone signaling comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of (i) a PDE 1 Inhibitor, e.g., of Formula I, Ia, II, III, IV, V, VIIa, VIIb, VIIIa, VIIIb, IXa or IXb, or any of Formulae 1.2-1.17, 2.1-2.9, or 3.2-3.22, 4.1-4.16, 5.1-5.6; (ii) an intraocular pressure lowering agent to a patient in need thereof.
(141) The invention also comprises a method of treating glaucoma or glaucoma-like conditions comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of sss (i) a PDE 1 Inhibitor, e.g., of Formula X or XI or any of 6.1 or 7.1-7.12; (ii) an intraocular pressure lowering agent.
to a patient in need thereof.
(142) The invention also comprises a method of treating glaucoma or elevated intraocular pressure comprising administering simultaneously, sequentially, or contemporaneously administering therapeutically effective amounts of sss (i) a PDE 1 Inhibitor, e.g., of Formula XII-XIII or any of 8.1-8.30 (ii) an intraocular pressure lowering agent.
to a patient in need thereof.
(143) The present invention also provides
(144) (i) a PDE 1 Inhibitor for use in the treatment of glaucoma or glaucoma-like conditions, as hereinbefore set forth, or in a method of treatment as hereinbefore set forth;
(145) (ii) the use of a PDE 1 Inhibitor in the manufacture of a medicament for treating a glaucoma or glaucoma-like conditions, or manufacture of a medicament for use in a method of treatment as hereinbefore set forth; and
(146) (iii) a pharmaceutical composition comprising a PDE 1 Inhibitor in combination or association with a pharmaceutically acceptable diluent or carrier for use in the treatment of a glaucoma or elevated intraocular pressure as hereinbefore set forth, or for use in a method of treatment as hereinbefore set forth.
(147) The words “treatment” and “treating” are to be understood accordingly as embracing prophylaxis and treatment or amelioration of any of the symptoms of disease as well as treatment of the cause of the disease.
(148) Dosages employed in practicing the present invention will of course vary depending, e.g. on the particular disease or condition to be treated, the particular PDE 1 Inhibitor used, the mode of administration, and the therapy desired. PDE 1 Inhibitors may be administered by any suitable route, including orally, parenterally, transdermally, or by inhalation, but are preferably administered topically to the eyes. In general, satisfactory results, e.g. for the treatment of diseases as hereinbefore set forth are indicated to be obtained on oral administration at dosages of the order from about 0.01 to 2.0 mg/kg. In larger mammals, for example humans, an indicated daily dosage for oral administration will accordingly be in the range of from about 0.75 to 150 mg, conveniently administered once, or in divided doses 2 to 4 times, daily or in sustained release form. Unit dosage forms for oral administration thus for example may comprise from about 0.2 to 75 or 150 mg, e.g. from about 0.2 or 2.0 to 50, 75 or 100 mg of a PDE 1 Inhibitor, together with a pharmaceutically acceptable diluent or carrier therefor.
(149) Pharmaceutical compositions comprising PDE 1 Inhibitors may be prepared using conventional diluents or excipients and techniques known in the galenic art. Thus oral dosage forms may include tablets, capsules, creams, ointments, solutions, suspensions and the like. In addition, ophthalmic disorders other than glaucoma may be treated by the compositions disclosed.
EXAMPLE I
(150) 1. Measurement of PDE1B inhibition in Vitro Using IMAP Phosphodiesterase Assay Kit
(151) Phosphodiesterase 1B (PDE1B) is a calcium/calmodulin dependent phosphodiesterase enzyme that converts cyclic guanosine monophosphate (cGMP) to 5′-guanosine monophosphate (5′-GMP). PDE1B can also convert a modified cGMP substrate, such as the fluorescent molecule cGMP-fluorescein, to the corresponding GMP-fluorescein. The generation of GMP-fluorescein from cGMP-fluorescein can be quantitated, using, for example, the IMAP (Molecular Devices, Sunnyvale, Calif.) immobilized-metal affinity particle reagent.
(152) Briefly, the IMAP reagent binds with high affinity to the free 5′-phosphate that is found in GMP-fluorescein and not in cGMP-fluorescein. The resulting GMP-fluorescein—IMAP complex is large relative to cGMP-fluorescein. Small fluorophores that are bound up in a large, slowly tumbling, complex can be distinguished from unbound fluorophores, because the photons emitted as they fluoresce retain the same polarity as the photons used to excite the fluorescence.
(153) In the phosphodiesterase assay, cGMP-fluorescein, which cannot be bound to IMAP, and therefore retains little fluorescence polarization, is converted to GMP-fluorescein, which, when bound to IMAP, yields a large increase in fluorescence polarization (Δmp). Inhibition of phosphodiesterase, therefore, is detected as a decrease in Δmp.
(154) 2. Enzyme Assay
(155) Materials: All chemicals are available from Sigma-Aldrich (St. Louis, Mo.) except for IMAP reagents (reaction buffer, binding buffer, FL-GMP and IMAP beads), which are available from Molecular Devices (Sunnyvale, Calif.).
(156) Assay: 3′,5′-cyclic-nucleotide-specific bovine brain phosphodiesterase (Sigma, St. Louis, Mo.) is reconstituted with 50% glycerol to 2.5 U/ml. One unit of enzyme will hydrolyze 1.0 μmole of 3′,5′-cAMP to 5′-AMP per min at pH 7.5 at 30° C. One part enzyme is added to 1999 parts reaction buffer (30 μM CaCl.sub.2, 10 U/ml of calmodulin (Sigma P2277), 10 mM Tris-HCl pH 7.2, 10 mM MgCl.sub.2, 0.1% BSA, 0.05% NaN.sub.3) to yield a final concentration of 1.25 mU/ml. 99 μl of diluted enzyme solution is added into each well in a flat bottom 96-well polystyrene plate to which 1 μl of test compound dissolved in 100% DMSO is added. The compounds are mixed and pre-incubated with the enzyme for 10 min at room temperature.
(157) The FL-GMP conversion reaction is initiated by combining 4 parts enzyme and inhibitor mix with 1 part substrate solution (0.225 μM) in a 384-well microtiter plate. The reaction is incubated in dark at room temperature for 15 min. The reaction is halted by addition of 60 μl of binding reagent (1:400 dilution of IMAP beads in binding buffer supplemented with 1:1800 dilution of antifoam) to each well of the 384-well plate. The plate is incubated at room temperature for 1 hour to allow IMAP binding to proceed to completion, and then placed in an Envision multimode microplate reader (PerkinElmer, Shelton, Conn.) to measure the fluorescence polarization (Δmp).
(158) A decrease in GMP concentration, measured as decreased Δmp, is indicative of inhibition of PDE activity. IC.sub.50 values are determined by measuring enzyme activity in the presence of 8 to 16 concentrations of compound ranging from 0.0037 nM to 80,000 nM and then plotting drug concentration versus ΔmP, which allows IC.sub.50 values to be estimated using nonlinear regression software (XLFit; IDBS, Cambridge, Mass.).
(159) Alternative combinations and variations of the examples provided will become apparent based on this disclosure. It is not possible to provide specific examples for all of the many possible combinations and variations of the embodiments described, but such combinations and variations may be claims that eventually issue.