SYNTHESIS OF (-)-TRANS-DELTA-9-TETRAHYDROCANNABIVARIN (DELTA-9 THCV) AND ANALOGS THEREOF

Abstract

A method is provided for the synthesis of (-)-trans-.sup.9-tetrahydrocannabivarin (.sup.9-THCV) and analogs thereof such that in the reaction product, the molar ratio of the .sup.9 isomer to incidentally formed .sup.8 isomers is greater than 4:1. Synthesis is carried out by combining a selected cannabinoid reactant, e.g., cannabidivarin (CBDV) or an analog thereof, with an acid in a solvent for the cannabinoid reactant, wherein the acid comprises (i) a Lewis acid having an acid softness index value in the range of 10 G.sup.0.sub.f, M.sup.n+ to 150 G.sup.0.sub.f, M.sup.n+, (ii) a Brnsted acid having a pKa in the range of 4.0 to +4.0, or (iii) a combination of (i) and (ii), under reaction conditions comprising a reaction temperature in the range of 0 C. to 25 C. and a reaction time in the range of 1 hour to 24 hours. The reaction is thereafter quenched with base and the solvent removed, wherein the crude reaction product so provided may be purified, e.g., chromatographically purified. Also provided is a method for synthesizing .sup.9-THCV that further includes synthesis of the cannabinoid reactant. The invention additionally provides novel cannabinoid compositions that may be synthesized using the aforementioned methodology.

Claims

1. A method for synthesizing a .sup.9 cannabinoid having the structure of formula (II) ##STR00015## wherein: R.sup.1 is selected from C.sub.1-C.sub.12 hydrocarbyl, substituted C.sub.1-C.sub.12 hydrocarbyl, heteroatom-containing C.sub.1-C.sub.12 hydrocarbyl, and substituted heteroatom-containing C.sub.1-C.sub.12 hydrocarbyl, R.sup.2, R.sup.3, and R.sup.5 are independently selected from C.sub.1-C.sub.6 alkyl and substituted C.sub.1-C.sub.6 alkyl; m is zero, 1 or 2; and R.sup.4 is OH or OR.sup.6 wherein R.sup.6 is H, C.sub.1-C.sub.6 alkyl, Cs-C.sub.12 aryl, or a hydroxyl protecting group, with the proviso that when m is 2, the R.sup.4 may be the same or different, wherein the method comprises: (a) combining a cannabinoid reactant having the structure of formula (I) ##STR00016## with an acid in a solvent for the cannabinoid reactant and the acid to provide a reaction mixture, wherein the acid comprises (i) a Lewis acid having an acid softness index value in the range of 10 G.sup.0.sub.f, M.sup.n+ to 150 G.sup.0.sub.f, M.sup.n+, (ii) a Brnsted acid having a pka in the range of 4.0 to +4.0, or (iii) a combination of (i) and (ii), under reaction conditions comprising a reaction temperature in the range of-0 C. to 25 C. and a reaction time in the range of 1 hour to 24hours; (b) quenching the reaction mixture of (a) with a base; and (c) removing the solvent to provide a reaction product comprising the .sup.9 cannabinoid having the structure of formula (II).

2. The method of claim 1, wherein: R.sup.1 is selected from C.sub.1-C.sub.8 alkyl, substituted C.sub.1-C.sub.8 alkyl, heteroatom-containing C.sub.1-C.sub.8 alkyl, substituted heteroatom-containing C.sub.1-C.sub.8 alkyl, C.sub.2-C.sub.8 alkenyl, substituted C.sub.2-C.sub.8 alkenyl, heteroatom-containing C.sub.2-C.sub.8 alkenyl, and substituted heteroatom-containing C.sub.2-C.sub.8 alkenyl; R.sup.2 and R.sup.3 are C.sub.1-C.sub.6 alkyl and may be the same or different; R.sup.5 is H; and m is zero.

3. The method of claim 2, wherein the reaction product further comprises .sup.8 isomers of the compound of formula (II), wherein the .sup.8 isomers have the structure of formula (II-A), (II-B), and (II-C) ##STR00017##

4. The method of claim 3, wherein, in the reaction product, the .sup.9 cannabinoid having the structure of formula (II) is in a molar ratio, relative to the .sup.8 isomers of formulae (II-A), (II-B), and (II-C), of greater than 4:1.

5. The method of claim 4, wherein the molar ratio of the .sup.9 cannabinoid to the .sup.8 isomers in the reaction product is in the range of 4:1 to 50:1.

6. The method of claim 5, wherein the molar ratio of the .sup.9 cannabinoid to the .sup.8 isomers in the reaction product is in the range of 9:1 to 18:1.

7. The method of claim 3, wherein the acid comprises a Lewis acid having an acid softness index value in the range of 10 G.sup.0.sub.f, M.sup.n+ to 150 G.sup.0.sub.f, M.sup.n+.

8. The method of claim 7, wherein the Lewis acid comprises a salt of a Group 13 cation, a transition metal in the +2 oxidation state, a transition metal in the +3 oxidation state, an actinide, or a lanthanide.

9. The method of claim 8, wherein the salt comprises a halide.

10. The method of claim 9, wherein the salt comprises a chloride selected from AlCl.sup.3, BCl.sup.3, GaCl.sup.3, InCl.sup.3, ZnCl.sup.2, TiCl.sup.4, MnCl.sup.2, FeCl.sup.2, FeCl.sup.3, LaCl.sup.3, and AcCl.sup.3.

11. (canceled)

12. The method of claim 3, wherein the acid comprises a Brnsted acid having a pKa in the range of 4.0 to +4.0.

13. (canceled)

14. The method of claim 3, wherein the reaction conditions further comprise a concentration of the cannabinoid reactant in the solvent in the range of 0.25 M to 2.5 M.

15. (canceled)

16. The method of claim 3, wherein the acid is present in the reaction mixture at a molar ratio in the range of 0.01:1 to 0.2:1 relative to the cannabinoid reactant.

17. (canceled)

18. (canceled)

19. The method of claim 3, wherein: R.sup.1 is selected from C.sub.1-C.sub.8 alkyl and C.sub.2-C.sub.8 alkenyl, optionally substituted with (a) (CO)NR.sup.8-R.sup.9 wherein R.sup.8 and R.sup.9 are independently selected from H and C.sub.1-C.sub.12 hydrocarbyl, (b) NR.sup.10R.sup.11 wherein R.sup.10 is H or C.sub.1-C.sub.12 hydrocarbyl and R.sup.11 is C.sub.6-C.sub.12 hydrocarbyl, C.sub.1-C.sub.12 hydrocarbyl substituted with at least one functional group, C.sub.1-C.sub.12 heterohydrocarbyl, or C.sub.1-C.sub.12 heterohydrocarbyl substituted with at least one functional group, (c) (SO.sub.2)R.sup.12 wherein R.sup.12 is H or C.sub.1-C.sub.12 heterohydrocarbyl, C.sub.1-C.sub.12 hydrocarbyl substituted with at least one functional group, or C.sub.1-C.sub.12 heterohydrocarbyl substituted with at least one functional group, (d) (SO.sub.2)NR.sup.13R.sup.14 wherein R.sup.13 is H or C.sub.1-C.sub.12 hydrocarbyl and R.sup.14 is H or C.sub.1-C.sub.12 hydrocarbyl, ##STR00018## wherein L.sup.1 is C.sub.1-C.sub.6 alkyl; and R.sup.2 and R.sup.3 are methyl.

20. The method of claim 19, wherein R.sup.1 is C.sub.1-C.sub.8 alkyl or C.sub.2-C.sub.8 alkenyl.

21. (canceled)

22. The method of claim 20, wherein R.sup.1 is n-propyl, such that the cannabinoid reactant having the structure of formula (I) is cannabidivarin and the -9 cannabinoid having the structure of formula (II) is .sup.9-tetrahydrocannabivarin.

23-26. (canceled)

27. The method of claim 3, further including purifying the reaction product composition to provide a purified reaction product.

28-30. (canceled)

31. The method of claim 27, wherein the molar ratio of the .sup.9 cannabinoid to the .sup.8 isomers in the purified reaction product is in the range of 50:1 to 1000:1.

32. A cannabinoid composition comprising .sup.9-THCV, .sup.8-THCV, .sup.8-iso-THCV, .sup.4(8)-iso-THCV, and a Lewis acid catalyst residue, wherein the molar ratio of the .sup.9-THCV to the total of the .sup.8-THCV, .sup.8-iso-THCV, and .sup.4(8)-iso-THCV is greater than 4:1 and the Lewis acid catalyst residue represents 1-150 ppm of the composition.

33-34. (canceled)

35. The purified cannabinoid composition of claim 32, wherein the molar ratio of the .sup.9-THCV to the total of the .sup.8-THCV, .sup.8-iso-THCV, and .sup.4(8)-iso-THCV is in the range of 50:1 to 1000:1.

36. (canceled)

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0038] FIG. 1 is a .sup.1H NMR spectrum of a reaction product generated using the synthetic method described in Examples 1-3.

[0039] FIG. 2 is a high-performance liquid chromatogram for an early eluting fraction of the purified reaction product of Example 4.

[0040] FIG. 3 is a high-performance liquid chromatogram for a late eluting fraction of the purified reaction product of Example 4.

[0041] FIG. 4 is a process flow diagram for the synthesis of .sup.9-THCV as described in Example 6.

DETAILED DESCRIPTION OF THE INVENTION

I. Definitions and Nomenclature

A. General

[0042] Unless defined otherwise, all technical and scientific terms used herein have the meaning commonly understood by one of ordinary skill in the art to which the invention pertains. Specific terminology of particular importance to the description of the present invention is defined below.

[0043] In this specification and the appended claims, the singular forms a, an and the include plural referents unless the context clearly dictates otherwise.

B. Chemical Terminology

[0044] As used herein, the phrase having the formula or having the structure is not intended to be limiting and is used in the same way that the term comprising is used.

[0045] The term hydrocarbyl refers to hydrocarbyl groups or linkages containing 1 to about 18 carbon atoms, typically 1 to 12 carbon atoms, including linear, branched, cyclic, saturated, and unsaturated species, such as alkyl groups, alkenyl groups, aryl groups, and the like. Substituted hydrocarbyl refers to hydrocarbyl substituted with one or more substituent groups, and the term heteroatom-containing hydrocarbyl refers to hydrocarbyl in which at least one carbon atom is replaced with a heteroatom. Unless otherwise indicated, the term hydrocarbyl is to be interpreted as including substituted and/or heteroatom-containing hydrocarbyl moieties.

[0046] The term alkyl as used herein refers to a branched or unbranched saturated hydrocarbon group typically although not necessarily containing 1 to about 18 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, octyl, decyl, and the like, as well as cycloalkyl groups such as cyclopentyl, cyclohexyl, and the like. Generally, although again not necessarily, alkyl groups herein contain 1 to 12 carbon atoms, e.g., 1 to 12 carbon atoms, 1 to 10 carbon atoms, 1 to 8 carbon atoms, 1 to 6 carbon atoms or 1 to 3 carbon atoms. Substituted alkyl refers to alkyl substituted with one or more substituent groups, and the terms heteroatom-containing alkyl and heteroalkyl refer to alkyl in which at least one carbon atom is replaced with a heteroatom, as described in further detail infra. If not otherwise indicated, the term alkyl includes linear, branched, cyclic, unsubstituted, substituted, and/or heteroatom-containing alkyl.

[0047] The term alkenyl as used herein refers to a linear, branched, or cyclic hydrocarbon group of 2 to about 18 carbon atoms containing at least one double bond, such as ethenyl, n-propenyl, isopropenyl, n-butenyl, isobutenyl, octenyl, decenyl, tetradecenyl, hexadecenyl, eicosenyl, tetracosenyl, and the like. Alkenyl groups herein typically contain 2 to 12 carbon atoms, e.g., 2 to 12 carbon atoms, 2 to 10 carbon atoms, 2 to 8 carbon atoms, 2 to 6 carbon atoms or 2 to 3 carbon atoms. The term cycloalkenyl intends a cyclic alkenyl group, typically having 5 to 8 carbon atoms. The term substituted alkenyl refers to alkenyl substituted with one or more substituent groups, and the terms heteroatom-containing alkenyl and heteroalkenyl refer to alkenyl in which at least one carbon atom is replaced with a heteroatom. If not otherwise indicated, the term alkenyl includes linear, branched, cyclic, unsubstituted, substituted, and/or heteroatom-containing alkenyl.

[0048] The term alkynyl as used herein refers to a linear or branched hydrocarbon group of 2 to 18 carbon atoms containing at least one triple bond, such as ethynyl, n-propynyl, and the like. Generally, although again not necessarily, alkynyl groups herein contain 2 to 12 carbon atoms, e.g., 2 to 12 carbon atoms, 2 to 10 carbon atoms, 2 to 8 carbon atoms, 2 to 6 carbon atoms or 2 to 3 carbon atoms. The term substituted alkynyl refers to alkynyl substituted with one or more substituent groups, and the terms heteroatom-containing alkynyl and heteroalkynyl refer to alkynyl in which at least one carbon atom is replaced with a heteroatom. If not otherwise indicated, the term alkynyl includes linear, branched, unsubstituted, substituted, and/or heteroatom-containing alkynyl.

[0049] The term alkoxy as used herein refers to an alkyl group bound through a single, terminal ether linkage; that is, an alkoxy group may be represented as O-alkyl where alkyl is as defined above. Alkoxy groups thus include C.sub.1-C.sub.6 alkoxy groups such as methoxy, ethoxy, n-propoxy, isopropoxy, t-butyloxy, etc. The terms alkenyloxy and alkynyloxy are defined in an analogous manner.

[0050] The term aryl as used herein, and unless otherwise specified, refers to an aromatic substituent containing a single aromatic ring or multiple aromatic rings that are fused together, directly linked, or indirectly linked (such that the different aromatic rings are bound to a common group such as a methylene or ethylene moiety). Preferred aryl groups contain 5 to 18 carbon atoms, and particularly preferred aryl groups contain 5 to 14 carbon atoms. Exemplary aryl groups contain one aromatic ring or two fused or linked aromatic rings, e.g., phenyl, naphthyl, biphenyl, diphenylether, diphenylamine, benzophenone, and the like. Substituted aryl refers to an aryl moiety substituted with one or more substituent groups, and the terms heteroatom-containing aryl and heteroaryl refer to aryl substituent, in which at least one carbon atom is replaced with a heteroatom, as will be described in further detail infra. If not otherwise indicated, the term aryl includes unsubstituted, substituted, and/or heteroatom-containing aromatic substituents.

[0051] The term aryloxy as used herein refers to an aryl group bound through a single, terminal ether linkage, wherein aryl is as defined above. An aryloxy group may be represented as O-aryl where aryl is as defined above. Preferred aryloxy groups contain 5 to 18carbon atoms, and particularly preferred aryloxy groups contain 5 to 14 carbon atoms. Examples of aryloxy groups include, without limitation, phenoxy, o-halo-phenoxy, m-halo-phenoxy, p-halo-phenoxy, o-methoxy-phenoxy, m-methoxy-phenoxy, p-methoxy-phenoxy, 2,4-dimethoxy-phenoxy, 3,4,5-trimethoxy-phenoxy, and the like.

[0052] The term alkaryl refers to an aryl substituent that is substituted with an alkyl group, and the term aralkyl refers to an alkyl substituent that is substituted with an aryl group, wherein aryl and alkyl are as defined above. Preferred alkaryl and aralkyl groups contain 6 to 18 carbon atoms, and particularly preferred aralkyl groups contain 6 to 16 carbon atoms. For example, alkaryl groups include, for example, p-methylphenyl, 2,4-dimethylphenyl, p-cyclohexylphenyl, 2,7-dimethylnaphthyl, 7-cyclooctyinaphthyl, 3-ethyl-cyclopenta-1,4-diene, and the like. Aralkyl groups include, without limitation, benzyl, 2-phenyl-ethyl, 3-phenyl-propyl, 4-phenyl-butyl, 5-phenyl-pentyl, 4-phenylcyclohexyl, 4-benzylcyclohexyl, 4-phenylcyclohexylmethyl, 4-benzylcyclohexylmethyl, and the like. The terms alkaryloxy and aralkyloxy refer to substituents of the formula-OR wherein R is alkaryl or aralkyl, respectively, as just defined.

[0053] The term acyl refers to substituents having the formula (CO)-alkyl, (CO)-aryl, or (CO)-aralkyl, and the term acyloxy refers to substituents having the formula O(CO)-alkyl, O(CO)-aryl, or O(CO)-aralkyl, wherein alkyl, aryl, and aralkyl are as defined above.

[0054] The term cyclic refers to alicyclic or aromatic substituents that may or may not be substituted and/or heteroatom containing, and that may be monocyclic, bicyclic, or polycyclic.

[0055] The term alicyclic is used in the conventional sense to refer to an aliphatic cyclic moiety, as opposed to an aromatic cyclic moiety, and may be monocyclic, bicyclic, polycyclic, and may be bridged.

[0056] The terms halo and halogen are used in the conventional sense to refer to a chloro, bromo, fluoro, or iodo substituent.

[0057] The term heteroatom-containing as in a heteroatom-containing alkyl group (also termed a heteroalkyl group) or a heteroatom-containing aryl group (also termed a heteroaryl group) refers to a molecule, linkage, or substituent in which one or more carbon atoms are replaced with an atom other than carbon, e.g., nitrogen, oxygen, sulfur, phosphorus, or silicon, typically nitrogen, oxygen, or sulfur, preferably nitrogen or oxygen. Similarly, the term heteroalkyl refers to an alkyl substituent that is heteroatom-containing, the term heterocyclic refers to a cyclic substituent that is heteroatom-containing, the terms heteroaryl and heteroaromatic respectively refer to aryl and aromatic substituents that are heteroatom-containing, and the like. Examples of heteroalkyl groups include alkoxyaryl, alkylsulfanyl-substituted alkyl, N-alkylated amino alkyl, and the like. Examples of heteroaryl substituents include pyrrolyl, pyrrolidinyl, pyridinyl, quinolinyl, indolyl, pyrimidinyl, imidazolyl, 1,2,4-triazolyl, tetrazolyl, etc., and examples of heteroatom-containing alicyclic groups are pyrrolidino, morpholino, piperazino, piperidino, etc.

[0058] By substituted as in substituted alkyl, substituted aryl, and the like, as alluded to in some of the aforementioned definitions, is meant that in the alkyl, aryl, or other moiety, at least one hydrogen atom bound to a carbon (or other) atom is replaced with one or more non-hydrogen substituents. Examples of such substituents include functional groups and hydrocarbyl moieties.

[0059] Functional groups that may represent substituents in the substituted molecular structures and segments thereof include, without limitation: halo, hydroxyl, sulfhydryl, C.sub.1-C.sub.18 alkoxy, C.sub.2-C.sub.18 alkoxyalkyl, C.sub.2-C.sub.18 alkenyloxy, C.sub.2-C.sub.18 alkynyloxy, C.sub.5-C.sub.18 aryloxy, acyl (including C.sub.2-C.sub.18 alkylcarbonyl (CO-alkyl) and C.sub.6-C.sub.18 arylcarbonyl (CO-aryl)), acyloxy (-O-acyl), C.sub.2-C.sub.18 alkoxycarbonyl ((CO)-O-alkyl), C.sub.6-C.sub.18 aryloxycarbonyl ((CO)O-aryl), halocarbonyl (CO)X where X is halo), C.sub.2-C.sub.18 alkylcarbonato (O(CO)O-alkyl), C.sub.6-C.sub.18 arylcarbonato (O(CO)O-aryl), carboxy (COOH), carboxylato (COO), carbamoyl ((CO)NH.sub.2), mono-(C.sub.1-C.sub.18 alkyl)-substituted carbamoyl (-(CO)-NH (C.sub.1-C.sub.18 alkyl)), di-(C.sub.1-C.sub.18 alkyl)-substituted carbamoyl (-(CO)-N (C.sub.1-C.sub.18 alkyl).sub.2), mono-(C.sub.5-C.sub.18 aryl)-substituted carbamoyl ((CO)NH-aryl), di-(C.sub.5-C.sub.18 aryl)-substituted carbamoyl ((CO)N(aryl).sub.2), di-N-(C.sub.1-C.sub.18 alkyl), N-(C.sub.5-C.sub.18 aryl)-substituted carbamoyl, thiocarbamoyl ((CS)NH.sub.2), carbamido (NH(CO)NH.sub.2), cyano (CN), isocyano (N.sup.+C.sup.), cyanato (OCN), isocyanato (ON.sup.+C.sup.), isothiocyanato (SCN), azido (NN.sup.+N.sup.), formyl ((CO)H), thioformyl ((CS)H), amino (NH.sub.2), mono-(C.sub.1-C.sub.18 alkyl)-substituted amino, di-(C.sub.1-C.sub.18 alkyl)-substituted amino, mono-(C.sub.5-C.sub.18 aryl)-substituted amino, di-(C.sub.5-C.sub.18 aryl)-substituted amino, C.sub.2-C.sub.18 alkylamido (NH(CO)-alkyl), C.sub.6-C.sub.18 arylamido (NH(CO)-aryl), imino (CRNH where R=hydrogen, C.sub.1-C.sub.18 alkyl, C.sub.5-C.sub.18 aryl, C.sub.6-C.sub.18 alkaryl, C.sub.6-C.sub.18 aralkyl, etc.), alkylimino (CRN(alkyl), where R=hydrogen, C.sub.1-C.sub.18 alkyl, C.sub.5-C.sub.18 aryl, C.sub.6-C.sub.18 alkaryl, C.sub.6-C.sub.18 aralkyl, etc.), arylimino (CRN(aryl), where R=hydrogen, C.sub.1-C.sub.18 alkyl, C.sub.5-C.sub.18 aryl, C.sub.6-C.sub.18 alkaryl, C.sub.6-C.sub.18 aralkyl, etc.), nitro (NO.sub.2), nitroso (NO), sulfo (SO.sub.2OH), sulfonato (SO.sub.2O), C.sub.1-C.sub.18 alkylsulfanyl (-S-alkyl; also termed alkylthio), arylsulfanyl (-S-aryl; also termed arylthio), C.sub.1-C.sub.18 alkylsulfinyl ((SO)-alkyl), C.sub.5-C.sub.18 arylsulfinyl ((SO)-aryl), C.sub.1-C.sub.18 alkylsulfonyl (SO.sub.2-alkyl), C.sub.5-C.sub.18 arylsulfonyl (SO.sub.2-aryl), phosphono (P(O)(OH).sub.2), phosphonato (P(O)(O).sub.2), phosphinato (P(O)(O)), phospho (PO.sub.2), and phosphino (PH.sub.2). Typically, hydrocarbyl moieties in the aforementioned functional groups, if acyclic, have 1 to 12 carbon atoms, while if cyclic, have 5 to 16 carbon atoms.

[0060] The aforementioned functional groups may, if a particular group permits, be further substituted with one or more additional functional groups or with one or more hydrocarbyl moieties such as those specifically enumerated above, and the term functional group encompasses all such instances.

[0061] When the term substituted appears prior to a list of possible substituted groups, it is intended that the term apply to every member of that group. For example, the phrase substituted alkyl, alkenyl, and aryl is to be interpreted as substituted alkyl, substituted alkenyl, and substituted aryl. Analogously, when the term heteroatom-containing appears prior to a list of possible heteroatom-containing groups, it is intended that the term apply to every member of that group. For example, the phrase heteroatom-containing alkyl, alkenyl, and aryl is to be interpreted as heteroatom-containing alkyl, substituted alkenyl, and substituted aryl.

[0062] An analog of a compound is one that has a structure similar to that of the compound but differs in one or more respects. For instance, a compound and an analog thereof may differ with respect to an atom, a substituent (which may or may not be a functional group), or a molecular segment. An analog may or may not be a derivative of the compound; it may be derived from the compound or it may be independently synthesized from one or more different reactants. An analog may be a homologue of a compound, meaning that the compound and the analog differ by a repeating unit (e.g., a methylene group).

[0063] Some of the compounds described herein may contain one or more asymmetric centers and give rise to enantiomers, diastereomers, or other stereoisomeric forms. Such a compound may be in the form of a single stereoisomer, i.e., be stereoisomerically pure, or contained in a mixture of two or more stereoisomers, e.g., two diastereomers, two enantiomers, or a mixture of two diastereomers and two enantiomers.

[0064] However, .sup.9-THCV and analogs thereof, as referred to herein and unless otherwise specified, are in the following configuration (benzopyran numbering convention included):

##STR00005##

II. The Synthetic Method

[0065] The novel synthetic method results in a reaction product that comprises a .sup.9 cannabinoid having the structure of formula (II)

##STR00006##

wherein: [0066] R.sup.1 is selected from C.sub.1-C.sub.12 hydrocarbyl, substituted C.sub.1-C.sub.12 hydrocarbyl, heteroatom-containing C.sub.1-C.sub.12 hydrocarbyl, and substituted heteroatom-containing C.sub.1-C.sub.12 hydrocarbyl, [0067] R.sup.2, R.sup.3, and R.sup.5 are independently selected from C.sub.1-C.sub.6 alkyl and substituted C.sub.1-C.sub.6 alkyl; [0068] m is zero, 1 or 2; and [0069] R.sup.4 is OH or OR.sup.6 wherein R.sup.6 is H, C.sub.1-C.sub.6 alkyl, Cs-C.sub.12 aryl, or a hydroxyl protecting group, with the proviso that when m is 2, the R.sup.4 may be the same or different.

[0070] The synthesis uses, as a starting material, a cannabinoid reactant having the structure of formula (I)

##STR00007##

wherein all substituents are defined as for compound (II), and is carried out using reaction parameters and reaction conditions that result in a reaction product in which compound (II) predominates relative to incidentally produced by-products, particularly .sup.8 isomers of (II).

[0071] In some embodiments, the synthetic method of the invention comprises: [0072] (a) combining the cannabinoid reactant of formula (I) with an acid in a solvent for the cannabinoid reactant and the acid to provide a reaction mixture, wherein the acid comprises (i) a Lewis acid having an acid softness index value in the range of 10 G.sup.0.sub.f, M.sup.n+ to 150 G.sup.0.sub.f, M.sup.n+, (ii) a Brnsted acid having a pKa in the range of 4.0 to +4.0, or (iii) a combination of (i) and (ii), under reaction conditions comprising a reaction temperature in the range of 0 C. to 25 C. and a reaction time in the range of 1 hour to 24 hours; [0073] (b) quenching the reaction mixture of (a) with a base; and [0074] (c) removing the solvent to provide a reaction product comprising the .sup.9 cannabinoid having the structure of formula (II).

[0075] The cannabinoid reactant of formula (I) may be obtained from a natural product, i.e.,

[0076] hemp, or it may be synthesized in whole or in part. For example, the cannabinoid reactant may be chemically synthesized using the methodology described in applicant's published international patent application WO 2022/133332 A2 and in co-pending U.S. patent application Ser. No. 18/212,061, filed Jun. 20, 2023 and published on Nov. 9, 2023 as US 2023/0357177 A1, previously incorporated by reference herein. When the cannabinoid reactant is CBDV per se (i.e., the compound of formula (I) wherein R.sup.1 is n-propyl, R.sup.2 and R.sup.3 are methyl), m is zero, and R.sup.5 is H), a representative synthesis thereof comprises contacting divarinol with .sup.9-2,8-menthadien-1-ol in the presence of a Lewis acid catalyst under reaction conditions effective to result in a reaction product comprising cannabidivarin. Another representative synthesis of the CBDV reactant starts with phloroglucinol and comprises: contacting phloroglucinol with a hydroxyl-protecting reagent to provide hydroxyl-protected phloroglucinol; effecting a cross-coupling reaction of the hydroxyl-protected phloroglucinol with a reactant M-CH.sub.2CH.sub.2CH.sub.3 in the presence of a catalyst that facilitates the cross-coupling reaction, wherein M comprises a metallic element, to provide hydroxyl-protected divarinol; deprotecting the hydroxyl-protected divarinol; and contacting the divarinol with .sup.9-2,8-menthadien-1-ol in the presence of a Lewis acid catalyst under reaction conditions effective to result in a reaction product comprising cannabidivarin. Further detail may be found in the aforementioned patent applications.

[0077] In some embodiments, the R.sup.1 substituent indicated in the molecular structures of formula (I) and (II) is selected from C.sub.1-C.sub.8 alkyl, substituted C.sub.1-C.sub.8 alkyl, heteroatom-containing C.sub.1-C.sub.8 alkyl, substituted heteroatom-containing C.sub.1-C.sub.8 alkyl, C.sub.2-C.sub.8 alkenyl, substituted C.sub.2-C.sub.8 alkenyl, heteroatom-containing C.sub.2-C.sub.8 alkenyl, and substituted heteroatom-containing C.sub.2-C.sub.8 alkenyl, while R.sup.2 and R.sup.3 are C.sub.1-C.sub.6 alkyl and may be the same or different; R.sup.5 is H; and m is zero. In some embodiments, R.sup.1 is C.sub.1-C.sub.8 alkyl or C.sub.2-C.sub.8 alkenyl. In other embodiments, R1 is C.sub.2-C.sub.6 alkyl. It should be noted that when R.sup.1 is n-propyl, R.sup.2 and R.sup.3 are methyl, m is zero, and R.sup.5 is H, the cannabinoid reactant is cannabidivarin (CBDV).

[0078] In the initial step of the reaction, the cannabinoid reactant is added to a solvent so that the concentration of the cannabinoid reactant therein is in the range of 0.25 M to 2.5 M, generally 0.25 M to 1.5 M. Any solvent or solvent combination used should dissolve the reactants and be compatible with all components of the reaction mixture without adversely affecting the intended reaction or any reactant. Representative solvents that can be used include, but are not limited to, aromatic hydrocarbons, e.g., toluene, ethyl benzene, and xylenes; alkanes such as cyclohexane; ketones, e.g., acetone, methyl ethyl ketone, diethyl ketone, methyl n-propyl ketone, acetophenone, and cyclohexanone; ethers, including linear, poly and cyclic ethers such as diethyl ether, di-n-propyl ether, di-n-butyl ether, methyl t-butyl ether, ethyl n-propyl ether, glyme, diglyme, tetrahydrofuran, 1,4-dioxane, and 1,3-dioxolane; lower (C.sub.1-C.sub.6) alcohols, e.g., methanol, ethanol, n-propanol, isopropanol, isomers of butanol, isomers of pentanol, ethylene glycol, propylene glycol, and glycerol; and other solvents such as dimethylformamide (DMF), dimethoxyethane (DME), dimethylacetamide (DMAC), N-methylpyrrolidone (NMP), dimethylsulfoxide (DMSO); chloroform; and dichloromethane (DCM), dichloroethane (DCE), acetonitrile (MeCN), ethyl acetate (EtOAc), and propylene carbonate.

[0079] Two or more solvents can be combined into a single solvent system, where the two or more solvents may or may not be selected from the foregoing list.

[0080] A preferred solvent is typically, although not necessarily, an aprotic, polar solvent that, in one embodiment, is non-coordinating; illustrative preferred solvents are chlorinated solvents such as DCM, DCE, and chloroform, as well as aromatic solvents such as toluene and benzene.

[0081] After combining the cannabinoid reactant and the solvent, the mixture is cooled to a temperature below 0 C., typically in the range of 25 C. to 0 C. After cooling to a reaction temperature in the aforementioned range, the selected acid is added to the reaction mixture in order to facilitate the intramolecular cyclization reaction represented in Scheme 1:

##STR00008##

[0082] The primary parameters relied upon to select a suitable acid for catalyzing the above reaction are Lewis acid hardness and Brnsted acid pKa. Suitable Lewis acids are those having an Acid Softness Index in the range of 10 G.sup.0.sub.f, M.sup.n+ to 150 G.sup.0.sub.f, M.sup.n+, while suitable Brnsted acids are those having a pka in the range of 4.0 to +4.0, e.g., 2.0 to +2.0. A combination of two or more acids may also be used, e.g., two or more Lewis acids, two or more Brnsted acids, or a combination of at least one Lewis acid with at least one Brnsted acid.

[0083] Suitable Lewis acids are generally selected from the following: a salt of a Group 13 element of the periodic table (also referred to as Group IIIB) such as Al, B, Ga, In, and Tl; a salt of a transition metal in the +2 or +3 oxidation state, such as Zn, Ti, Mn, Fe.sup.2+, and Fe.sup.3+; or a salt of an actinide or lanthanide. Halide salts, e.g., chloride salts, may be preferred in some embodiments. By way of illustration, representative Lewis acids that can be advantageously used in the present method include AlCl.sub.3, BCl.sub.3, GaCl.sub.3, InCl.sub.3, ZnCl.sub.2, TiCl.sub.4, MnCl.sub.2, FeCl.sub.2, FeCl.sub.3, LaCl.sub.3, and AcCl.sub.3.

[0084] Suitable Brnsted acids for use herein, as noted above, are those having a pKa in the range of 4.0 to +4.0, e.g., 2.0 to +2.0, insofar as acids with pKa value above these ranges may not be strong enough to promote isomerization (see the mechanism illustrated in Scheme 1), while acids with a pka value below these ranges may lead to over-isomerization, in turn resulting in generation of a larger fraction of .sup.8 isomers in the reaction product. Suitable Brnsted acids herein include, without limitation, sulfonic acids such as p-toluenesulfonic acid (tosic acid), methanesulfonic acid, ethanesulfonic acid, 1-hexanesulfonic acid, trifluoromethanesulfonic acid, benzenesulfonic acid, 4-ethylbenzenesulfonic acid, 1,5-naphthalenesulfonic acid, and camphorsulfonic acid; carboxylic acids such as trichloroacetic acid, trifluoroacetic acid, oxalic acid, fumaric acid, phthalic acid, and formic acid; inorganic acids such as hydrochloric acid, sulfuric acid, nitric acid, and phosphoric acid; and acidic resins.

[0085] Representative Brnsted acids and their pKa values are set forth in Table 1:

TABLE-US-00001 TABLE 1 Acid pKa p-Toluenesulfonic acid 2.8 Methanesulfonic acid 2.0 Benzenesulfonic acid 0.60 Trifluoroacetic acid 0.3 Trichloroacetic acid 0.65 Camphorsulfonic acid 1.2 Ethanesulfonic acid 1.83 2-Nitrobenzoic acid 2.17 2-Hydroxybenzoic acid 2.98 1-Hexanesulfonic acid 3.6 Formic acid 3.75

[0086] The relative quantities of the cannabinoid reactant and the acid are, like other reaction parameters discussed herein, selected to maximize the relative amount of the desired .sup.9 isomer in the reaction product, i.e., the amount relative to the less desirable .sup.8. Generally, the acid is present in the reaction mixture at a molar ratio in the range of 0.01:1 to 0.2:1 relative to the cannabinoid reactant (i.e., 1 mol % to 20 mol %), including a range of 0.05:1 to 0.1:1 (i.e., 5 mol % to 10 mol %), and a range of 0.07:1 to 0.1:1 (i.e., 7 mol % to 10 mol %).

[0087] After addition of the selected acid to the cooled cannabinoid reactant/solvent mixture, the resulting reaction mixture is stirred for an additional 1 to 24 hours, typically, although not necessarily, for an additional 1 to 8 hours, with, optionally, an increase in temperature partway through (e.g., stirring at 25 C. for 1.5 hours followed by stirring at 0 C. for an additional 4.5 hours; see Example 1). Higher temperatures should be avoided, insofar as higher temperatures lead to a precipitous drop in the .sup.9:.sup.8. At this point, the reaction is presumed to be complete and the reaction mixture is quenched with a suitable base, e.g., sodium bicarbonate. The solvent layer is removed using conventional means such as evaporation under vacuum, resulting in generation of the crude reaction product that comprises the desired .sup.9 isomer having the structure of formula (II). Temperatures greater than 70 C. should be avoided in the solvent removal step; optimally, solvent removal should be carried out at a temperature of at most 50 C.

[0088] It will be understood that in some embodiments, the cannabinoid reactant has the structure of formula (I-A) while the desired reaction product, i.e., a .sup.9 cannabinoid encompassed by the generic structure of formula (II), has the structure of formula (II-D)

##STR00009##

wherein, in some embodiments, R.sup.1 is C.sub.1-C.sub.8 alkyl or C.sub.2-C.sub.8 alkenyl, e.g., C.sub.2-C.sub.6 alkyl, such as n-propyl; and R.sup.2 and R.sup.3 are C.sub.1-C.sub.6 alkyl and may be the same or different.

[0089] In this embodiment, the reaction product comprises, in addition to the desired reaction product (II-D), incidentally produced by-products, i.e., the .sup.8 isomers of (II-D), having the structures of formulae (II-A), (II-B), and (II-C), as follows:

##STR00010##

[0090] In one representative embodiment, R.sup.1 is n-propyl and R.sup.2 and R.sup.3 are methyl, such that the cannabinoid reactant is cannabidivarin (CBDV) and the desired reaction product is .sup.9-tetrahydrocannabivarin (.sup.9-THCV)

##STR00011##

while the .sup.8 isomers of formulae (II-A), (II-B), and (II-C) are .sup.8-THCV, .sup.8-iso-THCV, and .sup.4(8)-iso-THCV:

##STR00012##

The above-described synthetic method reduces the relative quantity of the less desired .sup.8 isomers (compounds II-A, II-B, and II-C, e.g., .sup.8-THCV, .sup.8-iso-THCV, and A4 (8)-iso-THCV, respectively) in the reaction product, i.e., relative to the desired .sup.9 isomer (compound II, e.g., compound II-D, exemplified by .sup.9-THCV). That is, the present method results in a reaction product in which the desired .sup.9 cannabinoid having the structure of formula (II) is in a molar ratio, relative to the .sup.8 isomers of formulae (II-A), (II-B), and (II-C), of greater than 4:1. In some embodiments, the molar ratio of the .sup.9 cannabinoid to the .sup.8 isomers in the reaction product is in the range of 4:1 to 50:1. In some embodiments, the molar ratio of the .sup.9 cannabinoid to the .sup.8 isomers in the reaction product is in the range of 9:1 to 18:1.

[0091] The aforementioned molar ratios obtained are for the crude reaction product that results from the final step of the reaction, solvent removal. Purification of the crude reaction product can result in significantly higher molar ratios of the desired .sup.9 cannabinoid to the .sup.8 isomers, on the order of 50:1 or greater, e.g., in the range of 50:1 to 1000:1 or greater.

[0092] Following completion of synthesis and generation of compound (I) as the desired reaction product, compound (I) may be modified, if desired, to provide further analogs, using techniques known to those of ordinary skill in the art, described in the pertinent literature and texts, or developed hereinafter.

III. Purification of the Reaction Product

[0093] The reaction product obtained using the method of the preceding section can be purified so as to enrich the fraction of the desired .sup.9 cannabinoid therein and provide a purified reaction product composition in which significantly higher molar ratios of the .sup.9 cannabinoid product to the .sup.8 isomers are achieved. It will be appreciated that various purification techniques can be implemented to achieve the desired result, and will be known to those of ordinary skill in the art or inferred from the pertinent literature. A preferred method, however, is described in Example 4, infra. The method comprises chromatographically purifying the reaction product using normal phase silica column chromatography and a simple isocratic mobile phase, e.g., a mixture of non-polar and polar organic solvents where the non-polar solvent may be heptane, hexane, petroleum ether, toluene, or the like, and polar solvent may be acetone, ethyl acetate, dichloromethane, methyl-t-butyl ether, or the like. One representative solvent combination, as documented in the Examples herein, is a mixture of heptane and acetone. Purification of gram to kilogram quantities of synthetically generated .sup.9 cannabinoid (e.g., .sup.9 THCV) can be achieved using commercially available or other pre-packed silica columns, up to 3 kg in size, using bulk silica. The isocratic mobile phase enables retention of cannabinoids, removal of residual solvent, and isolation of the desired .sup.9 cannabinoid essentially free from starting materials and minor impurities. The use of an isocratic mobile phase also facilitates recovery and reuse of the solvents used for chromatographic separation.

IV. Cannabinoid Compositions

[0094] The invention also encompasses a novel composition of matter, a cannabinoid composition comprising .sup.9-THCV, .sup.8-THCV, .sup.8-iso-THCV, A4 (8)-iso-THCV, and a Lewis acid catalyst residue, wherein the molar ratio of the .sup.9-THCV to the total of the .sup.8-THCV, .sup.8-iso-THCV, and .sup.4(8)-iso-THCV is greater than 4:1 (e.g., in the range of 4:1 to 50:1, such as 9:1 to 18:1) and the Lewis acid catalyst residue, e.g., aluminum or another metal deriving from the selected acid used in the reaction, represents 1-150 ppm of the composition.

[0095] In another embodiment, the invention encompasses as a novel composition of matter a purified cannabinoid composition comprising .sup.9-THCV, .sup.8-THCV, .sup.8-iso-THCV, .sup.4(8)-iso-THCV, and a Lewis acid catalyst residue, wherein the molar ratio of the .sup.9-THCV to the total of the .sup.8-THCV, .sup.8-iso-THCV, and .sup.4(8)-iso-THCV is greater than 50:1 (e.g., in the range of 50:1 to 1000:1) and the Lewis acid catalyst residue, which again may be aluminum or another metal deriving from the acid used in the reaction, represents 1-150 ppm of the composition.

[0096] It is to be understood that while the invention has been described in conjunction with a number of specific embodiments, the foregoing description as well as the examples that follow are intended to illustrate and not limit the scope of the invention.

[0097] All patents, patent publications, literature references, and other materials cited herein are incorporated by reference in their entireties.

EXAMPLE 1

Synthesis of .SUP.9.-THCV From Cannabidivarin (CBDV)

[0098] 2 kg of CBDV 2 kg of CBDV was added to a 50 L jacketed reactor. The reactor was charged with 20 L of dichloromethane (DCM), giving a CBDV concentration of 0.35M. The reaction mixture was cooled to 25 C., before AlCl.sub.3 (6 mol % of CBDV, i.e., an AlCl3:CBDV molar ratio of 0.06:1) was added to the reactor. The mixture was stirred at 150 rm for 1.5 hours before increasing the temperature to 0 C. Stirring continued at this temperature for 4.5 hours. The reaction was quenched by the addition of sodium bicarbonate. The DCM layer was dried using sodium sulfate and evaporated under a vacuum to produce the final THCV resin.

EXAMPLE 2

[0099] The procedure of Example 1 was followed except that the concentration of CBDV in the reaction mixture was 0.7 M.

EXAMPLE 3

[0100] The procedure of Example 1 was followed except that amount of AlCl.sub.3 used was increased from 6 mol % to 7 mol. %.

Results

[0101] Analysis of the reaction product composition obtained in Examples 1 through 3 was carried out, with the results provided in Table 2:

TABLE-US-00002 TABLE 2 .sup.8-THCV and Total Total THCV .sup.8-THCV cannabinoids, obtained, .sup.9-THCV, isomers, .sup.9:.sup.8 Example wt. % wt. % wt. % wt. % ratio 1 89.48 83.13 74.60 8.53 8.74 2 97.15 85.77 79.76 6.01 13.24 3 99.9 98.83 92.99 5.84 15.92

[0102] In the above table, the .sup.8-THCV isomers refer to .sup.8-iso-THCV and .sup.4(8)-iso-THCV.

[0103] The .sup.1H NMR spectrum of the reaction product composition obtained in Example 3 is attached as FIG. 1 (.sup.1H NMR (400 MHz, CDCl.sub.3) 6.31 (s, 1H), 6.27 (s, 1H), 4.79 (s, 1H), 3.20 (d, J=10.79 Hz, 1 H), 2.42 (m, 2H), 2.116 (d, J =6.02 Hz, 2H), 1.92 (m, 1H), 1.68 (s, 3H), 1.58 (m, 2H), 1.42 (s,3H), 1.10 (s, 3H), 0.92 (t, J =7.29 Hz, 3H)). As indicated therein, the reaction product composition was primarily composed of .sup.9-THCV, with several minor peaks indicating the presence of solvent, unreacted CBDV reactant, and .sup.8-THCV isomers.

EXAMPLE 4

Purification of .SUP.9.-THCV

[0104] The .sup.9-THCV reaction product obtained in Example 2 was purified using normal phase silica column chromatography, as follows:

[0105] Fractions of heptane-acetone eluant were collected in an automated fashion during normal phase chromatographic separation of the D.sup.9-THCV reaction product synthesized as described in Example 2, using the puriFlash ultra performance flash purification system (Advion Interchim Scientific, Ithaca, New York). The isolated fractions were then evaporated to dryness, reconstituted in methanol, and analyzed for cannabinoid content and purity by reverse-phase high pressure liquid chromatography (HPLC). The results of the HPLC analysis demonstrated that the reactant and trace impurities were concentrated in the early eluting fractions, allowing the isolation of later eluting fractions containing highly purified D.sup.9-THCV. More specifically, and as illustrated in FIG. 2, HPLC analysis of Fraction 6 shows the presence of starting material (CBDV eluting at 1.870 min), the desired final product (D.sup.9-THCV eluting at 2.806 min), and minor impurities eluting at 2.401 and 2.941 min). In contrast, the HPLC analysis of Fraction 12, as may be seen in FIG. 3, demonstrates the presence of only one component, highly purified D.sup.9-THCV (eluting at 2.793 min).

EXAMPLE 5

Synthesis and Purification of CBDV Reactant

[0106] CBDV was synthesized according to Scheme 2, below.

##STR00013##

[0107] To an oven-dried 5 L round bottom flask equipped with an overhead mechanical stirrer, a thermocouple, and a heating mantle, was added pre-dried divarinol (523.5 g, 3.44 mol) and 2.4 L of dry 1,2-dichloroethane. The solution was stirred for 5 minutes. Basic alumina (105.7 g, 1.04 mol) and magnesium sulfate (332.0 g, 2.76 moles) were added, and the suspension was stirred for another 5 minutes, after which BF.sub.3.Math.OEt.sub.2 (13.1 mL, 0.106 mol) was added. The suspension was stirred 15 minutes under nitrogen, at room temperature.

[0108] The reaction mixture was then heated at 70 C., and a solution of (1S, 4R)-p-mentha-2,8-dien-1-ol (209.9 g, 1.37 mol) in 380 mL of anhydrous 1,2-dichloroethane was added in on portion. A slight increase of temperature was observed (5 C.). The mixture was stirred for 30 seconds, after which 1.2 L of a saturated aqueous solution of sodium bicarbonate was slowly added. The mixture was cooled to room temperature. The pH was measured with litmus paper to ensure that the aqueous layer was alkaline.

[0109] The entire reaction mixture was filtered on a Celite 545 pad to retrieve most of the alumina. The filtrate was inserted in an extraction funnel, and the phases were allowed to separate. The aqueous phase was extracted with 500 ml of fresh DCM. The organic phases were combined, washed with 1L of an aqueous saturated solution of sodium chloride, and dried with magnesium sulfate. After filtration on Whatman #1 filter paper, the organic fraction was evaporated to dryness, providing 754.2 g of a brown oil.

[0110] The residue was dissolved in 3.1 L of heptane and mechanically stirred for 5 minutes with a solution made of 1.7 L of methanol, 1.4 L of water, and 32 mL of an aqueous saturated solution of sodium chloride. The phases were separated using a separatory funnel. The organic layer was dried with magnesium sulfate, suction filtered on a Celite 545 pad, and evaporated to dryness.

[0111] A portion of 200 ml of fresh heptane was added to the residue and evaporated to dryness. A viscous beige solid (310.0 g) was obtained on which a portion of 550 mL of heptane was added. The heterogenous mixture was allowed to stir mechanically overnight at room temperature. It was then suction filtered on a Whatman #1 filter, providing 119.2 g of a beige powder, containing 97% of CBDV and 3% of abn CBDV, as confirmed by 1H-NMR. (The filtrate (179.4 g) contained 27% of CBDV, 61% of abn CBDV, and 12% of bis compound.)

[0112] The beige solid was recrystallized at room temperature in 1170 mL of heptane, providing 109.3 g of light-yellow needles. The heptane suspension was heated to 80 C. in order to ensure total solubility. Crystallization began at 50 C.

EXAMPLE 6

Synthesis and Purification of .SUP.9.-THCV

[0113] This example describes synthesis of .sup.9-THCV according to Scheme 3, below.

##STR00014##

[0114] CBDV (1 equiv.), prepared as described in Example 5, was added to the glass reactor previously inerted to less than 1% O.sub.2, followed by (2.7 vol.) of anhydrous DCM. The mixture was cooled to between 30 and 25 C. before gradually adding AlCl.sub.3 (0.07 equiv.) in three equal portions with a 20-minute interval between additions, during which the reaction mixture changed from gold yellow/brown to bright red/pink/brown. After maintaining the mixture at 25 C. for an hour, the reactor was set to 0 C. and the reaction was held over five hours. Post-reaction, the mixture was washed twice with 1 volume of 10% sodium bicarbonate solution, each wash followed by settling and separation of the lower DCM layer, which was retained and the upper aqueous layer discarded. Sodium sulfate (1.17 equiv.) and basic aluminum oxide Brockman 1 (2.09 equiv.) were then added to the retained DCM layers, mixed for 1 hour, and the resulting solution was filtered using a Buchner funnel with Whatman Grade 4 cellulose filter paper (20-25 m). The filtrate was evaporated under vacuum at 50 C. for 3-4 hours in a rotary evaporator, reconstituted in equal parts of 98% +Ethanol (1 L ethanol per kg of resin), and concentrated again to a honey-like consistency at 60 C. The final product was spread in thin layers and dried overnight at 60 C., yielding over 2.5 kg of .sup.9-THCV, which was analyzed by HPLC-DAD, HS-GC-MS and 1H-NMR. The final .sup.9-THCV product was a very viscous glass-like amorphous resin at room temperature, which was observed to freeze and shatter after storing at 20 C. for 6+hrs. The typical color was transparent golden yellow, but the purple color appeared rapidly when the surface contacted the non-inert atmosphere. This color change was found to have minimal effect on the quality of the product.

[0115] A process flow diagram for synthesis of .sup.9-THCV is included in FIG. 4. Table 3, below, provides the results of in-process control (IPC) and release tests:

TABLE-US-00003 TABLE 3 In-process Control (IPC) and Release Tests Acceptable Step Test Range Reaction Thin Layer Chromatography (TLC) No CBDV observed Quench pH for catalyst deactivation pH > 7.4 Release Assay, appearance, related substances, See detailed residual solvents, metals & bioburden specifications