IMMOBILIZED MINING MICROBIAL ACCELERATOR BASED ON BIOSURFACTANT BACTERIA AND PREPARATION METHOD THEREOF

Abstract

The present disclosure relates to an immobilized mining microbial accelerator based on biosurfactant bacteria, including immobilized biosurfactant bacteria, immobilized mineralized bacteria, an activating solution and a cementing agent. The immobilized mining microbial accelerator based on biosurfactant bacteria is prepared by using the following steps: firstly, inoculating biosurfactant bacteria and mineralized bacteria into the activating solution to obtain a biosurfactant bacterium fermentation solution and a mineralized bacterium fermentation solution; and then, selectively adsorbing and enriching the biosurfactant bacteria and the mineralized bacteria through an immobilized material to obtain the immobilized biosurfactant bacteria and the immobilized mineralized bacteria. The hydrophobicity of the immobilized mining microbial accelerator can be improved when the microbial accelerator is applied to coal dust, and further, the improvement of the mineralization capacity and the transformation of the shape of CaCO.sub.3 crystals can be achieved through mutualism between two strains of bacteria.

Claims

1. An immobilized mining microbial accelerator based on biosurfactant bacteria, comprising immobilized biosurfactant bacteria, immobilized mineralized bacteria, an activating solution and a cementing agent, wherein the activating solution and the cementing agent are respectively independently packaged before the use. a preparation method of the immobilized biosurfactant bacteria or the immobilized mineralized bacteria comprises the following steps: (1) adjusting the pH of the activating solution with NaOH to be neutral, performing sterilization, then inoculating the activated immobilized biosurfactant bacteria or the activated immobilized mineralized bacteria into the sterilized activating solution, and performing incubation in an incubator to obtain a biosurfactant bacterium fermentation solution and a mineralized bacterium fermentation solution; (2) respectively sequentially performing operations of centrifugation, removal of a supernate and addition of a sterile saline solution on the biosurfactant bacterium fermentation solution and the mineralized bacterium fermentation solution obtained in the step (1); (3) after an immobilized material is sterilized, adding a biosurfactant bacterium suspension or a mineralized bacterium suspension, then adding the sterilized activating solution, and performing sealed constant-temperature incubation to obtain an immobilized biosurfactant bacterium solution or an immobilized mineralized bacterium solution; and (4) filtering the immobilized biosurfactant bacterium solution or the immobilized mineralized bacterium solution via a filter sieve, and lyophilizing the filtered immobilized biosurfactant bacterium solution or the filtered immobilized mineralized bacterium solution by utilizing alyophilizer to obtain the immobilized biosurfactant bacteria or the immobilized mineralized bacteria; and a preparation method of the immobilized material comprises the following steps: S1, sequentially performing operations of washing with distilled water, soaking with a NaOH aqueous solution, washing with deionized water and drying to obtain an alkali-treated immobilizer; S2, adding nanometer titanium dioxide particles and a super-hydrophobic coating material into an activating solution (ethyl alcohol) and uniformly dispersing the nanometer titanium dioxide particles and the super-hydrophobic coating material to obtain a milky white suspension; and S3, soaking the alkali-treated immobilizer obtained in the step S1 in the suspension obtained in the step S2, and then performing drying to obtain the immobilized material.

2. The immobilized mining microbial accelerator according to claim 1, wherein a ratio of the total mass of the immobilized biosurfactant bacteria and the immobilized mineralized bacteria to volumes of the activating solution and the cementing agent is (1 g to 3 g) to (65 mL to 75 mL) to (5 mL to 10 mL).

3. The immobilized mining microbial accelerator according to claim 1, wherein in the step (1), the sterilization is performed at a temperature of 121 C. for 20 min; and the incubation is performed at a temperature of 30 C. and a stirring rotating speed of 150 rpm for 48 h.

4. The immobilized mining microbial accelerator according to claim 1, wherein in the step (2), the biosurfactant bacterium suspension and the mineralized bacterium suspension have concentrations of 110.sup.7 CFU/mL to 110.sup.8 CFU/mL and 110.sup.8 CFU/mL to 110.sup.10 CFU/mL, respectively.

5. The immobilized mining microbial accelerator according to claim 1, wherein in the step (3), a volume ratio of the biosurfactant bacterium suspension or the mineralized bacterium suspension to the activating solution is 1 to (99 to 105).

6. The immobilized mining microbial accelerator according to claim 1, wherein in the step (3), the incubation is performed at a temperature of 25 C. and a stirring rotating speed of 150 rpm for 24 h.

7. The immobilized mining microbial accelerator according to claim 1, wherein in the step S1, the NaOH aqueous solution has a concentration of 1 mol/L to 1.2 mol/L, and the soaking time is 24 h; the number of times of washing is 3 to 5; and a lyophilization method is to perform drying to a constant weight in a vacuum lyophilization oven at a temperature of 65 C.

8. The immobilized mining microbial accelerator according to claim 1, wherein in the step S1, the immobilizer is at least one of loofah sponge, straw, bagasse and corncob, and has a particle size of 30 mm to 40 mm.

9. The immobilized mining microbial accelerator according to claim 1, wherein in the step S2, the super-hydrophobic coating material is at least one of paraffin, polytetrafluoroethylene and graphene.

10. The immobilized mining microbial accelerator according to claim 1, wherein the step S2 is to respectively add 2 g to 5 g of the nanometer titanium dioxide particles and 1 g of the super-hydrophobic coating material into 100 mL of ethyl alcohol and perform ultrasonic dispersion for 2 h to 4 h to obtain the milky white suspension.

11. The immobilized mining microbial accelerator according to claim 1, wherein in the step S3, the soaking time is 12 h, and the drying is performed in an oven of 60 C. for 24 h.

12. The immobilized mining microbial accelerator according to claim 1, wherein the biosurfactant bacteria are selected from at least one of Bacillus brevis, Pseudomonas aeruginosa, Bacillus licheniformis or Corynebacterium, and have a concentration of 110.sup.7 CFU/mL to 110.sup.8 CFU/mL; and the mineralized bacteria are selected from at least one of jelly-like Bacillus, Bacillus subtilis, Bacillus amyloliquefaciens, Sporosarcina pasteurii or Bacillus sphaericus, and have a concentration of 110.sup.8 CFU/mL to 110.sup.10 CFU/mL.

13. The immobilized mining microbial accelerator according to claim 1, wherein the activating solution contains NH.sub.4Cl, MnSO.sub.4.Math.H.sub.2O and NiCl.sub.2.Math.6H.sub.2O, and further contains a yeast extract or peptone.

14. The immobilized mining microbial accelerator according to claim 13, wherein the activating solution contains the following components in parts by weight: 2000 parts to 3000 parts of peptone, 1000 parts to 2000 parts of NH.sub.4Cl, 100 parts to 150 parts of MnSO.sub.4.Math.H.sub.2O and 200 parts to 300 parts of NiCl.sub.2.Math.6H.sub.2O.

15. The immobilized mining microbial accelerator according to claim 1, wherein the cementing agent contains a soluble calcium salt and urea.

16. The immobilized mining microbial accelerator according to claim 15, wherein the soluble calcium salt is at least one of C.sub.2H.sub.2O.sub.4Ca, CaCl.sub.2, C.sub.6H.sub.10CaO.sub.6 or C.sub.4H.sub.6CaO.sub.4.

17. The immobilized mining microbial accelerator according to claim 15, wherein the soluble calcium salt has a molar concentration of 0.8 mol/L to 1.0 mol/L.

18. The immobilized mining microbial accelerator according to claim 15, wherein a ratio of molar concentrations of the soluble calcium salt and the urea is 1 to 1.

19. An application of the immobilized mining microbial accelerator according to claim 1 for coal dust solidification, comprising the following steps: respectively adding immobilized biosurfactant bacteria and immobilized mineralized bacteria into a sterilized activating solution, and performing constant-temperature incubation to obtain a compound microbial fermentation solution; and sequentially spraying the compound microbial fermentation solution and a sterilized cementing agent to coal dust, wherein a manner of adding the immobilized biosurfactant bacteria and the immobilized mineralized bacteria into the sterilized activating solution is to firstly add 1 part of the immobilized biosurfactant bacteria into a sterilized activating solution and then add 1 part of the immobilized mineralized bacteria after the incubation is performed for 14 h, and continue to perform the incubation for 10 h; and a volume ratio of the compound microbial fermentation solution to the cementing agent is (13 to 15) to (1 to 2).

Description

BRIEF DESCRIPTION OF DRAWINGS

[0051] FIG. 1 shows growth curves of bacteria in a compound microbial fermentation solution under different inoculation sequences.

[0052] FIG. 2 shows amounts of CaCO.sub.3 produced by an immobilized mining microbial accelerator under different inoculation sequences.

[0053] FIG. 3 shows electron microscopy views of producing CaCO.sub.3 by an immobilized mining microbial accelerator under different inoculation sequences.

[0054] FIG. 4 shows contact angles of an immobilized mining microbial accelerator on coal under different inoculation sequences.

[0055] FIG. 5 shows wind erosion resistances of pulverized coal after being treated with an immobilized mining microbial accelerator under different inoculation sequences.

[0056] FIG. 6 shows a production apparatus of a material described in an embodiment 1 of the present disclosure, wherein 1, constant-temperature oscillation incubator; 2, connection tube; 3, immobilized bacterium solution storage tank; 4, filter vibration sieve; 5, conveyor belt; and 6, lyophilizer.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0057] The present disclosure will be further described below in conjunction with specific embodiments, and advantages and characteristics of the present disclosure will become apparent with the description. However, the embodiments are merely exemplary and do not constitute any limitation on the scope of the present disclosure. Those skilled in the art should understand that details and forms of the technical solution of the present disclosure may be modified or replaced without departing from the spirit and scope of the present disclosure, but these modifications and replacements fall within the scope of protection of the present disclosure.

Embodiment 1

[0058] Disclosed are an immobilized mining microbial accelerator based on biosurfactant bacteria and a preparation method thereof.

[0059] The preparation method of the immobilized mining microbial accelerator based on biosurfactant bacteria includes the following steps: [0060] I, preparing an immobilized material by the following steps: [0061] S1, washing loofah sponge with distilled water, soaking the washed loofah sponge in a 1 mol/L NaOH aqueous solution for 24 h, washing the loofah sponge 5 times with deionized water to remove the residual NaOH, and then, drying alkali-treated loofah sponge in a vacuum lyophilization oven of 65 C. to constant weight; [0062] S2, respectively adding 2 g of nanometer titanium dioxide particles and 1 g of a paraffin into 100 mL of ethyl alcohol and performing ultrasonic dispersion for 2 h to 4 h to obtain a milky white suspension; and [0063] S3, immersing alkali-treated loofah sponge in the suspension for 12 h, then drying the alkali-treated loofah sponge in an oven of 60 C. for 24 h to obtain the immobilized material; [0064] II, preparing immobilized biosurfactant bacteria and immobilized mineralized bacteria by the following steps: [0065] (1) adjusting the pH of 2000 parts of a yeast extract, 1000 parts of NH.sub.4Cl, 100 parts of MnSO.sub.4.Math.H.sub.2O and 200 parts of NiCl.sub.2.Math.6H.sub.2O to be 7 with 1 mol/L NaOH, performing sterilization in a vertical autoclave of 121 C. for 20 min, respectively inoculating activated Pseudomonas aeruginosa and activated Sporosarcina pasteurii into a sterilized activating solution by utilizing a pipette, and performing incubation in a constant-temperature oscillation incubator 1 under incubation conditions of 150 rpm and 30 C. for 48 h, to obtain a biosurfactant bacterium fermentation solution and a mineralized bacterium fermentation solution respectively; [0066] (2) centrifuging 30 mL of the biosurfactant bacterium fermentation solution and the mineralized bacterium fermentation solution under a condition of 8000 rpm for 5 min to remove a supernatant; adding 5 mL of a sterile saline solution and performing centrifugation under a condition of 8000 rpm for 5 min, removing the supernatant after the washed bacteria are precipitated twice, and adding 5 mL of the sterile saline solution to obtain a homogeneous biosurfactant bacterium suspension and a homogeneous mineralized bacterium suspension; [0067] (3) weighing two parts of 1.0 g, 3.0 g, 5.0 g, 7.0 g and 10.0 g of a washed and dried immobilized material and placing the weighed washed and dried immobilized material into two 100 mL conical flasks, performing sterilization in an autoclave for 20 min, respectively adding 1 mL of the biosurfactant bacterium suspension and the mineralized bacterium suspension to the conical flasks by using a pipette, adding 99 mL of an activating solution into each conical flask and sealing a mouth of the conical flask with a parafilm, and performing incubation under conditions 25 C. and 150 rpm in a constant-temperature oscillation incubator 1 for 24 h to obtain an immobilized biosurfactant bacterium solution and an immobilized mineralized bacterium solution; and [0068] (4) conveying the immobilized biosurfactant bacterium solution and the immobilized mineralized bacterium solution to an immobilized bacterium solution storage tank 3 through a connection tube 2, and filtering the immobilized biosurfactant bacterium solution and the immobilized mineralized bacterium solution by utilizing a filter vibration sieve 4, and conveying the filtered immobilized biosurfactant bacterium solution and the filtered immobilized mineralized bacterium solution to a lyophilizer through a conveyor belt 5 for being lyophilized to obtain the immobilized biosurfactant bacteria and the filtered immobilized mineralized bacteria; and [0069] III, weighing 1 g of the immobilized biosurfactant bacteria, 1 g of the immobilized mineralized bacteria, 75 mL of the activating solution and 5 mL of a cementing agent to obtain an immobilized mining biosurfactant, wherein the activating solution and the cementing agent are respectively independently packaged.

Experiment 1

[0070] The immobilized biosurfactant bacterium solution and the immobilized mineralized bacterium solution obtained in the step II (3) were left to stand. A supernatant was collected and centrifuged under a condition of 2000 rpm for 10 min. A final sample was collected at 1 cm below a surface of the supernatant. The number of microbial cells adsorbed on the surface of an immobilized material was calculated by measuring an OD value of the sample. Results are shown in Table 1.

TABLE-US-00001 TABLE 1 Microbial adsorption capacities of immobilized materials of different masses Mass (g) of immobilized material 1.0 3.0 5.0 7.0 10.0 Adsorption capacity 25.67 40.29 39.18 32.41 21.85 (mg/g) of immobilized biosurfactant bacteria Adsorption capacity 17.89 26.73 28.52 23.64 15.34 (mg/g) of immobilized mineralized bacteria

[0071] Table 1 shows that the number of the microbial cells adsorbed by the immobilized material shows a trend of first increasing and then decreasing with the increase of the mass of the immobilized material. The adsorption capacity is first increased and then decreased with the increase of the mass of coal dust, which may be due to a fact that the number of the microbial cells is not sufficient to adapt to the increase of the immobilized material, resulting in the decrease in the overall adsorption capacity. Among them, when the mass of the immobilized material ranges from 3.0 g to 5.0 g, the number of the microbial cells adsorbed by the immobilized material reaches the highest value of 39.18 mg/g to 40.29 mg/g and 26.73 mg/g to 28.52 mg/g, respectively. Therefore, the mass of the immobilized material selected in 100 mL of a bacterium solution (110.sup.8 CFU/mL) ranges from 3.0 g to 5.0 g when the immobilized biosurfactant bacteria and immobilized mineralized bacteria are prepared.

Experiment 2

[0072] The immobilized biosurfactant bacteria and the immobilized mineralized bacteria are the same as in the embodiment 1.

[0073] 1.0 g, 2.0 g, 3.0 g, 4.0 g, 5.0 g, 6.0 g, 7.0 g and 8.0 g of the immobilized biosurfactant bacteria and the immobilized mineralized bacteria were weighed according to a mass ratio of 1 to 1, and placed in 70 mL of an activating solution after being sterilized. Mouths were sealed with a parafilm. Incubation was performed in a constant-temperature oscillation incubator 1 under conditions of 25 C. and 150 rpm for 48 h. An OD value of a sample was measured. Growth characteristics of the immobilized bacteria of different masses were reflected according to the OD value. Results are shown in Table 2.

TABLE-US-00002 TABLE 2 Growth of immobilized bacteria of different masses Mass (g) of immobilized bacteria 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 OD.sub.600 1.287 1.293 1.307 1.286 1.279 1.274 1.268 1.251

[0074] Table 2 shows that the growth of the immobilized bacteria of different masses shows a trend of first increasing and then decreasing with the increase of the mass of the immobilized bacteria, which may be due to a fact that the growth of the bacteria in the activating solution is increased with the increase of the mass of the immobilized bacteria. However, nutrients in the activating solution are limited, which leads to a competitive relationship between the bacteria. The growth of the bacteria is decreased when the initial addition of the immobilized bacteria is too high. Therefore, the total mass of the immobilized biosurfactant bacteria and the immobilized mineralized bacteria ranges from 1.0 g to 3.0 g when the incubation is performed with the immobilized bacteria.

Experiment 3

[0075] 1.0 g of immobilized biosurfactant bacteria and/or 1.0 g of immobilized mineralized bacteria were weighed and inoculated into 75 mL of an activating solution after being sterilized, and a group of microbial fermentation solutions was set according to the inoculation time of the bacteria: X, X.sub.14P, X.sub.24P, P, P.sub.14X, P.sub.24X and PX, wherein P indicates inoculation of the immobilized biosurfactant bacteria, X indicates inoculation of the immobilized mineralized bacteria, 14 indicates inoculation of one strain of bacteria 14 h after inoculation of another strain of bacteria, and 24 indicates inoculation of one strain of bacteria 24 h after inoculation with another strain of bacteria. The microbial fermentation solutions were placed and incubated under conditions of 150 rpm and 30 C. in a constant-temperature oscillation incubator for 48 h. At set intervals within 48 h, the microbial fermentation solution in a 200 L conical flask was taken from an ultra-clean bench, the absorbance at a wavelength of 600 nm was measured by using a microplate reader, and growth curves of the bacteria are reflected. The measured results are shown in FIG. 1, which shows growth curves of the bacteria in a microbial fermentation solution under different inoculation sequences.

Experiment 4

[0076] 75 mL of the microbial fermentation solution prepared in the experiment 3 was added to 5 mL of a mixed solution of CaCl.sub.2 (36 g/L) and urea (20 g/L) after being sterilized by a filter head, and placed and incubated under conditions of 150 rpm and 30 C. in a constant-temperature oscillation incubator for mineralization for 7 days. A compound microbial fermentation solution after being subjected to the mineralization was filtered through filter paper, and dried with a drying oven at a temperature of 100 C. The total mass of the filter paper and precipitates after being dried was recorded as M.sub.1. The filter paper and the precipitates were washed with 0.7 mol/L hydrochloric acid. Precipitates of CaCO.sub.3 were removed, dried, weighed, and recorded as M.sub.2. The weight of the CaCO.sub.3 is recorded as M.sub.1-M.sub.2 (Table 3).

TABLE-US-00003 TABLE 3 Amounts of CaCO.sub.3 produced by immobilized mining microbial accelerator under different inoculation sequences Treatment Amounts (g/L) of precipitates of CaCO.sub.3 manner X X.sub.14P X.sub.24P P P.sub.14X P.sub.24X PX M.sub.1 M.sub.2 4.19 0.93 1.07 0.25 0.96 0.05 0.77 0.07 10.40 0. 7 2.20 0.22 4.31 0.19

[0077] FIG. 2 shows the amounts of the CaCO.sub.3 produced by the immobilized mining microbial accelerator under different inoculation sequences. Results show that after single immobilized bacteria and compound immobilized bacteria are mineralized after 7 days, the amounts of the CaCO.sub.3 produced by the immobilized mineralized bacteria X and the compound immobilized bacteria P.sub.14X and PX are high. The amount of the CaCO.sub.3 is 4.190.93 g/L after the immobilized mineralized bacteria X are mineralized for 7 days, which is similar to that of the compound immobilized bacteria PX (4.310.19 g/L). The amount of the CaCO.sub.3 produced by the compound immobilized bacteria P.sub.14X is 10.40.70 g/L, which is 148.21% and 141.30% higher than that of the immobilized mineralized bacteria X and the compound immobilized bacteria PX, respectively. The amount of the CaCO.sub.3 produced by the P.sub.24X is 2.200.22 g/L, which is lower than that of the P.sub.14X. These results indicate that the precipitation amount of the CaCO.sub.3 can be increased when the mineralized bacteria are inoculated 14 h after the biosurfactant bacteria are inoculated.

[0078] FIG. 3 shows electron microscopy views of CaCO.sub.3 produced by an immobilized mining microbial accelerator under different inoculation sequences, wherein A is X, B is X.sub.14P, C is X.sub.24P, D is P, E is P.sub.14X, F is a local enlarged view of P.sub.14X, G is P.sub.24X, and H is PX. It can be seen from the figure that a mineralized product produced by single immobilized bacteria is spherical and belongs to vaterite-type CaCO.sub.3. A mineralized product produced by compound immobilized bacteria is vaterite-type CaCO.sub.3 and calcite-type CaCO.sub.3. Among them, the compound immobilized bacteria P.sub.14X clearly show the coexistence of the vaterite-type CaCO.sub.3 and the calcite-type CaCO.sub.3, and the vaterite-type CaCO.sub.3 is gradually transformed into the stable calcite-type CaCO.sub.3, indicating that the stability of crystals of the CaCO.sub.3 can be improved when the mineralized bacteria are inoculated 14 h after the biosurfactant bacteria are inoculated.

Experiment 5

[0079] Pulverized coal (1 g, 200 mesh) was pressed into a pulverized coal sample at a pressure of 15 MPa through a tablet press to make a briquette. A contact angle (a seat drop method) test was performed by utilizing an optical contact angle gauge, and effects of biosurfactants produced by bacterium solutions incubated with different inoculation manners (the inoculation manners are the same as in the experiment 3) on the wettability of coal were compared. FIG. 4 shows effects of an immobilized mining microbial accelerator or water (W) on the wettability of coal under different inoculation sequences. Results show that a contact angle of water (W) on the surface of the coal ranges from 78.2 to 77.59, and a contact angle of an immobilized mineralized bacterium solution (X) on the surface of the coal ranges from 78.160 to 71.33. The contact angles of the immobilized mineralized bacterium solution (X) and water (W) on the surface of the coal are initially about 78, but the contact angle of the immobilized mineralized bacterium solution (X) is decreased within 1 min. In addition, the compound bacterium solution P.sub.14X has the best wettability for the coal. The tension of the surface of the coal is decreased by 34.27% after 1 min.

Experiment 6

[0080] A certain mass of pulverized coal was weighed and loaded into a graduated cylinder with h of 10100 mm, and tamped with a glass rod to make the height of the pulverized coal in the graduated cylinder be 10 cm. Moreover, 5 mL of microbial fermentation solutions under different inoculation manners (the inoculation manners are the same as in the experiment 3) was added for a permeation experiment. The permeation depth within 20 min was measured with a ruler. Results are shown in Table 4.

TABLE-US-00004 TABLE 4 Permeation depth of pulverized coal Treatment manner X X.sub.14P X.sub.24P P P.sub.14X P.sub.24X PX Permeation 1.18 3.49 3.83 2.53 5.02 4.34 4.62 depth (cm)

[0081] Table 4 shows that the permeation depth of a single immobilized bacterium solution is significantly increased compared with that of a compound immobilized bacterium solution. The permeation depth of the compound immobilized bacterium solution exhibits different permeability with different inoculation sequences. Among them, the permeability of the compound immobilized bacterium solution P.sub.14X is significantly higher than that of other compound immobilized bacterium solutions, which indicates that the generation of the immobilized biosurfactant bacteria is promoted when the immobilized mineralized bacteria are inoculated 14 h after the immobilized biosurfactant bacteria are inoculated.

Experiment 7

[0082] 18 mL of microbial fermentation solutions prepared under different inoculation manners (the inoculation manners are the same as in the experiment 3) were weighed and placed in a sprinkling can. 30 g of 120 mesh pulverized coal was weighed with a culture dish, 15 mL of microbial fermentation solutions prepared under different inoculation manners was sprinkled to the pulverized coal in the culture dish, and then 2 mL of a cementing agent, that is, 2 mL of a mixed solution of CaCl.sub.h(36 g/L) and urea (20 g/L) was sprinkled. Finally, the pulverized coal treated with an immobilized mining microbial accelerator was naturally air-dried at room temperature, and the above operations were repeated on the 3.sup.rd, 7.sup.th and 15.sup.th days, respectively. On the 20.sup.th day, a wind erosion resistance test was performed on the treated pulverized coal at a wind speed of 10 m/s. Results are shown in FIG. 5. FIG. 5 shows wind erosion resistances of the pulverized coal after being treated with the immobilized mining microbial accelerator under different inoculation manners. As can be seen from the figure that samples treated with water (W), an activating solution (C) and a cementing agent (J) have greater wind erosion mass loss compared to samples treated with the microbial accelerator. In addition, the wind erosion resistance of the compound immobilized bacterium solution is better than that of the single immobilized bacterium solution, which is due to a fact that the mass loss of the samples treated with a compound immobilized bacterium solution is significantly lower than that of samples treated with a single immobilized bacterium solution because of the dual action of microbial mineralization and a biosurfactant. In particular, the microbial accelerator prepared by P.sub.14X has good wind erosion resistance (1.550.91%), which is reduced by 25.014.44% compared with the microbial accelerator prepared by single immobilized mineralized bacteria. Due to different utilization capabilities of microorganisms to substrates, by inoculating and incubating the microorganisms in a certain order, not only can the synergistic metabolic effect between the microorganisms be fully exerted, but also the growth competition and suppression between the microorganisms can be avoided. In this way, higher biomass and yield are achieved.