Concentrated formic acid composition and its uses thereof

Abstract

The present invention is related to Methanoic acid composition and it's uses thereof. The Methanoic acid composition helps in reducing the germicidal load and finds utility in dental hygiene, Cosmetics, detergents, soaps, skin cleansers, surface cleaners, water sanitizers, animal feed, processed food, poultry farms, poultry hatcheries and the pharmaceutical arena.

Claims

1. A formic acid composition comprising formic acid ranging from 15.45 to 95 percent to the total weight of the composition and a compound selected from the group consisting of Sucrose, Glucose, Fructose, Lactose, Mannitol, Glycerin, Propylene glycol, Polyoxyethylene (20) sorbitan monooleate, Polyoxyl 40 Hydrogenated Castor Oil, Acetic Acid, Benzyl alcohol, Isopropyl alcohol, Polyethylene glycol, Ethanolamine, Diethanolamine, Triethanolamine, Oleic acid, Stearic acid and Palmitic acid or a combination thereof and balancing amount of water wherein the said product has a pH between 5 to 9 to enable reduction in microbial/germs load.

2. A process for the preparation of the formic acid composition as claimed in claim 1 wherein the said composition is obtained by mixing formic acid ranging from 15.45 to 95 percent to the total weight of the composition with a compound selected from the group consisting of Sucrose Glucose, Fructose, Lactose, Mannitol, Glycerin, Propylene glycol, Polyoxyethylene (20) sorbitan monooleate, Polyoxyl 40 Hydrogenated Castor Oil, Acetic Acid, Benzyl alcohol, Isopropyl alcohol, Polyethylene glycol, Ethanolamine, Diethanolamine, Triethanolamine, Oleic acid, Stearic acid and Palmitic acid or a combination thereof and balancing amount of water is added to result in a product having a pH between 5 to 9.

3. A process to assess the effectiveness of formic acid composition of claim 1 in reducing the microbe/germ load in a given substrate comprising the steps of; a) Preparing formic acid composition of by mixing formic acid ranging from 15.45 to 95 percent to the total weight of the composition with the compound selected from Sucrose, Glucose, Fructose, Lactose, Mannitol, Glycerin, Propylene glycol, Polyoxyethylene (20) sorbitan monooleate, Polyoxyl 40 Hydrogenated Castor Oil, Acetic Acid, Benzyl alcohol, Isopropyl alcohol, Polyethylene glycol, Ethanolamine, Diethanolamine, Triethanolamine, Oleic acid, Stearic acid and Palmitic acid or a combination thereof and balancing amount of water to make 100% total weight of the composition; b) Preparing media and inoculation culture by dissolving 4 gram of tryptone soya sugar in 100 ml of the composition of step (a) and keep it aside till agar to dissolve; c) sterilizing petri plate, inoculation loop and agar by autoclaving at 121 C. for 15 minutes; d) solidifying agar by pouring agar onto the petri plate; e) streaking microorganisms or germs in the agar; f) incubating the petri plate in an incubator for 2 days in 30-35 C. g) repeating the steps (b) to (f) with any other known disinfectant h) assessing and comparing the amount of formic acid composition of step (a) with the amount of any known disinfectant to achieve the nil growth of microorganisms/germs.

Description

EXAMPLE 1

(1) Aim: To compare the bactericidal activity of Methanoic acid composition solution along with other commercial disinfectants available in the market on the Pathogenic culture Pseudomonas aeruginosa.

(2) Scope:

(3) To determine the bactericidal activity of disinfectant solutions by streak plate method as per the procedure prescribed in the Pharmacopeia on the Pathogenic culture Pseudomonas aeruginosa.

(4) Media Used:

(5) Tryptone soya agar/Nutrient agar.

(6) Incubation Period:

(7) Incubate for 48 Hours at 30-35 C.

(8) Samples Preparation:

(9) Methanoic acid composition is processed by mixing 59 grams of Commercially available Methanoic acid of 85% concentration with 10 grams of betaD-Fructofuranosyl-(2.fwdarw.1)-alphaD-glucopyranoside and 31 grams of Dihydrogen monoxide. The other Commercial Disinfectants [represented herein by their Trade Names] are purchased directly from the market.

(10) The process adopted is by Trial and identification method.

(11) A known quantity of the Disinfectant is made up to 100 ml in a Sterile Standard Flask with water separately.

(12) Preparation of Media & Inoculation of Culture:

(13) 4 g of tryptone soya agar is dissolved in 100 ml of above dilute solutions separately and kept in a water bath for few minute for the agar to dissolve.

(14) Petri plate, inoculation loop & agar were autoclaved at 121 C. for 15 minutes for sterilization.

(15) The Sterile agar was then poured into the petri plate for solidification.

(16) After solidification the pathogenic culture namely Pseudomonas aeruginosa was streaked in the agar.

(17) The Petri plates were then incubated in the incubator for 2 days in 30-35 C.

(18) Result:

(19) The quantity of Disinfectant Required to ensure NIL GROWTH of the pathogen Pseudomonas aeruginosa in the Petri Plate is presented below.

(20) 1.3 gm of Methanoic Acid composition of Example 1 was sufficient to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate.

(21) 8 gms of Savlon was required to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate implying that the Methionic Acid composition presented in the present application is 6 times more potent than that of Savlon.

(22) 10 gms of Lizol was required to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate implying that the Methionic Acid composition presented in the present application is 7.6 times more potent than that of Lizol.

(23) 11 gms of Dettol was required to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate implying that the Methionic Acid composition presented in the present application is 8.4 times more potent than that of Dettol.

(24) 26 gms of Domex was required to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate implying that the Methionic Acid composition presented in the present application is 20 times more potent than that of Domex.

(25) 28 gms of Harpic was required to ensure NIL GROWTH of Pseudomonas aeruginosa in the Petri Plate implying that the Methionic Acid composition presented in the present application is 21.5 times more potent than that of Harpic.

EXAMPLE 2

(26) Aim: To compare the bactericidal activity of Methanoic acid composition solution along with other commercial disinfectants available in the market on the Pathogenic culture Staphylococcus aureus.

(27) Scope:

(28) To determine the bactericidal activity of disinfectant solutions by streak plate method as per the procedure prescribed in the Pharmacopeia on the Pathogenic culture Staphylococcus aureus.

(29) Media Used:

(30) Tryptone soya agar/Nutrient agar.

(31) Incubation Period:

(32) Incubate for 48 Hours at 30-35 C.

(33) Samples Preparation:

(34) Methanoic acid composition is processed by mixing 59 grams of Commercially available Methanoic acid of 85% concentration with 10 grams of (2R,3S,4R,5R)-2,3,4,5,6-Pentahydroxyhexanal and 31 grams of Dihydrogen monoxide. The other Commercial Disinfectants are purchased directly from the market.

(35) The process adopted is by Trial and identification method.

(36) A known quantity of the Disinfectant is made up to 100 ml in a Sterile Standard Flask with water separately.

(37) Preparation of Media & Inoculation of Culture:

(38) 4 g of tryptone soya agar is dissolved in 100 ml of above dilute solutions separately and kept in a water bath for few minute for the agar to dissolve.

(39) Petri plate, inoculation loop & agar were autoclaved at 121 C. for 15 minutes for sterilization.

(40) The Sterile agar was then poured into the petri plate for solidification.

(41) After solidification the pathogenic culture namely Staphylococcus aureus was streaked in the agar.

(42) The Petri plates were then incubated in the incubator for 2 days in 30-35 C.

(43) Result:

(44) The quantity of Disinfectant Required to ensure NIL GROWTH of the pathogen Staphylococcus aureus in the Petri Plate is presented below.

(45) 1.2 gm of Methanoic Acid composition was sufficient to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate.

(46) 9 gms of Savlon was required to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate implying that the Methionic Acid composition presented in the present application is 7.5 times more potent than that of Savlon.

(47) 10 gms of Lizol was required to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate implying that the Methionic Acid composition presented in the present application is 8.3 times more potent than that of Lizol.

(48) 11 gms of Dettol was required to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate implying that the Methionic Acid composition presented in the present application is 9.1 times more potent than that of Dettol.

(49) 27 gms of Domex was required to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate implying that the Methionic Acid composition presented in the present application is 22.5 times more potent than that of Domex.

(50) 29 gms of Harpic was required to ensure NIL GROWTH of Staphylococcus aureus in the Petri Plate implying that the Methionic Acid composition presented in the present application is 24.1 times more potent than that of Harpic.

EXAMPLE 3

(51) Aim: To compare the bactericidal activity of Methanoic acid composition solution along with other commercial disinfectants available in the market on the Pathogenic culture Escherichia coli.

(52) Scope:

(53) To determine the bactericidal activity of disinfectant solutions by streak plate method as per the procedure prescribed in the Pharmacopeia on the Pathogenic culture Escherichia coli.

(54) Media Used:

(55) Tryptone soya agar/Nutrient agar.

(56) Incubation Period:

(57) Incubate for 48 Hours at 30-35 C.

(58) Samples Preparation:

(59) Methanoic acid composition is processed by mixing 59 grams of Commercially available Methanoic acid of 85% concentration with 10 grams of (3S,4R,5R)-1,3,4,5,6-Pentahydroxyhexan-2-one and 31 grams of Dihydrogen monoxide. The other Commercial Disinfectants are purchased directly from the market.

(60) The process adopted is by Trial and identification method.

(61) A known quantity of the Disinfectant is made up to 100 ml in a Sterile Standard Flask with water separately.

(62) Preparation of Media & Inoculation of Culture:

(63) 4 g of tryptone soya agar is dissolved in 100 ml of above dilute solutions separately and kept in a water bath for few minute for the agar to dissolve.

(64) Petri plate, inoculation loop & agar were autoclaved at 121 C. for 15 minutes for sterilization.

(65) The Sterile agar was then poured into the petri plate for solidification.

(66) After solidification the pathogenic culture namely Escherichia coli was streaked in the agar.

(67) The Petri plates were then incubated in the incubator for 2 days in 30-35 C.

(68) Result:

(69) The quantity of Disinfectant required to ensure NIL GROWTH of the pathogen Escherichia coli in the Petri Plate is presented below.

(70) 1.3 gm of Methanoic Acid composition was sufficient to ensure NIL GROWTH of Escherichia coli in the Petri Plate.

(71) 9 gms of Savlon was required to ensure NIL GROWTH of Escherichia coli in the Petri Plate implying that the Methionic Acid composition presented in the present application is 6.9 times more potent than that of Savlon.

(72) 14 gms of Lizol was required to ensure NIL GROWTH of Escherichia coli in the Petri Plate implying that the Methionic Acid composition presented in the present application is 10.7 times more potent than that of Lizol.

(73) 18 gms of Dettol was required to ensure NIL GROWTH of Escherichia coli in the Petri Plate implying that the Methionic Acid composition presented in the present application is 13.8 times more potent than that of Dettol.

(74) 27 gms of Domex was required to ensure NIL GROWTH of Escherichia coli in the Petri Plate implying that the Methionic Acid composition presented in the present application is 20.7 times more potent than that of Domex.

(75) 29 gms of Harpic was required to ensure NIL GROWTH of Escherichia coli in the Petri Plate implying that the Methionic Acid composition presented in the present application is 22.3 times more potent than that of Harpic.

EXAMPLE 4

(76) Aim: To compare the bactericidal activity of Methanoic acid composition solution along with other commercial disinfectants available in the market on the Pathogenic culture Salmonella abony.

(77) Scope:

(78) To determine the bactericidal activity of disinfectant solutions by streak plate method as per the procedure prescribed in the Pharmacopeia on the Pathogenic culture Salmonella abony.

(79) Media Used:

(80) Tryptone soya agar/Nutrient agar.

(81) Incubation Period:

(82) Incubate for 48 Hours at 30-35 C.

(83) Samples Preparation:

(84) Methanoic acid composition is processed by mixing 59 grams of Commercially available Methanoic acid of 85% concentration with 10 grams of beta-D-galactopyranosyl-(1.fwdarw.4)-D-glucose and 31 grams of Dihydrogen monoxide. The other Commercial Disinfectants are purchased directly from the market.

(85) The process adopted is by Trial and identification method.

(86) A known quantity of the Disinfectant is made up to 100 ml in a Sterile Standard Flask with water separately.

(87) Preparation of Media & Inoculation of Culture:

(88) 4 g of tryptone soya agar is dissolved in 100 ml of above dilute solutions separately and kept in a water bath for few minute for the agar to dissolve.

(89) Petri plate, inoculation loop & agar were autoclaved at 121 C. for 15 minutes for sterilization.

(90) The Sterile agar was then poured into the petri plate for solidification.

(91) After solidification the pathogenic culture namely Salmonella abony was streaked in the agar.

(92) The Petri plates were then incubated in the incubator for 2 days in 30-35 C.

(93) Result:

(94) The quantity of Disinfectant Required to ensure NIL GROWTH of the pathogen Salmonella abony in the Petri Plate is presented below.

(95) 1.4 gm of Methanoic Acid composition was sufficient to ensure NIL GROWTH of Salmonella abony in the Petri Plate.

(96) 8 gms of Savlon was required to ensure NIL GROWTH of Salmonella abony in the Petri Plate implying that the Methionic Acid composition presented in the present application is 5.7 times more potent than that of Savlon.

(97) 10 gms of Lizol was required to ensure NIL GROWTH of Salmonella abony in the Petri Plate implying that the Methionic Acid composition presented in the present application is 7.1 times more potent than that of Lizol.

(98) 10 gms of Dettol was required to ensure NIL GROWTH of Salmonella abony in the Petri Plate implying that the Methionic Acid composition presented in the present application is 7.1 times more potent than that of Dettol.

(99) 28 gms of Domex was required to ensure NIL GROWTH of Salmonella abony in the Petri Plate implying that the Methionic Acid composition presented in the present application is 20 times more potent than that of Domex.

(100) 30 gms of Harpic was required to ensure NIL GROWTH of Salmonella abony in the Petri Plate implying that the Methionic Acid composition presented in the present application is 21.4 times more potent than that of Harpic.

EXAMPLE 5

(101) The samples of the present composition as indentified in below table were prepared and each sample is identified with an alphabet.

(102) TABLE-US-00001 TABLE 1 Sample A Sample B Sample C Sample D Sample E Sample F Sample G Sample H Weight % Weight % Weight % Weight % Weight % Weight % Weight % Weight % to the to the to the to the to the to the to the to the total total total total total total total total weight weight weight weight weight weight weight weight of the of the of the of the of the of the of the of the Ingredient composition composition composition composition composition composition composition composition Methanoic 95.00 82.75 76.57 55.65 49.95 32.80 22.77 15.45 acid with 85% purity beta-D- 0.32 1.25 2.02 NIL 4.55 3.45 8.98 7.45 Fructofuranosyl- (2.fwdarw.1)- alpha-D- glucopyranoside, (2R,3S,4R, 0.28 1.50 NIL 4.35 5.25 4.00 7.55 9.12 5R)- 2,3,4,5,6- Pentahydroxyhexanal, (3S,4R,5R)- 0.58 2.15 2.80 NIL 7.25 4.57 6.23 6.66 1,3,4,5,6- Pentahydroxyhexan-2- one beta-D- 0.44 2.11 0.68 NIL 4.98 3.20 8.34 6.77 galactopyranosyl- (1.fwdarw.4)-D- glucose (2S,3R,4R, 0.38 1.63 2.25 NIL 4.02 4.78 6.13 2.00 5R)- Hexane- 1,2,3,4,5,6- hexol Propane- 0.25 0.75 NIL 3.25 1.78 2.66 NIL 2.00 1,2,3-triol Propane- 0.25 1.06 NIL NIL 0.58 NIL 4.88 2.00 1,2-diol Polyoxyethylene 0.10 1.00 1.25 3.77 1.64 7.34 4.48 2.00 (20) sorbitan monooleate, Poly Oxyl 0.20 0.65 2.00 NIL 2.00 2.80 2.64 2.00 40 Hydrogenated Castor Oil Ethanoic 0.15 0.58 1.72 NIL 2.25 2.06 3.62 3.00 acid Phenylmethanol, 0.10 0.47 0.5 2.98 0.75 1.58 4.15 NIL Propan-2- 0.25 0.67 0.5 1.55 0.75 2.25 NIL 3.18 ol, poly(oxyethylene) 0.25 NIL 1.78 NIL 2.25 1.58 NIL 1.59 2- 0.20 NIL 2.00 NIL 1.58 2.27 NIL 2.98 Aminoethan- 1-ol, 2,2- 0.20 0.50 0.25 4.55 2.28 3.02 5.41 2.25 Iminodiethanol 2,2,2- 0.25 0.73 1.00 NIL 2.25 NIL 4.82 0.70 Nitrilotri(ethan- 1-ol), (9Z)- 0.10 NIL 1.25 3.90 0.09 NIL 4.44 NIL Octadec-9- enoic acid Octadecanoic 0.10 0.40 0.25 1.25 0.40 2.78 2.25 2.25 acid Hexadecanoic 0.20 0.80 0.03 1.25 0.40 3.34 0.31 4.05 acid Dihydrogen 0.40 1.00 3.15 17.5 5.00 15.52 3.00 24.55 monoxide

(103) The following Table-2 lists out the quantity of known disinfectants to ensure NIL GROWTH upon the pathogens tested as indicated therein.

(104) TABLE-US-00002 TABLE 2 Quantum of known Example disinfectant required to Number obtain NIL GROWTH Pathogen tested upon 1 a) 1.3 gm of Methanoic Psedomonas aeruginosa acid composition b) 8 g of SAVLON c) 10 g of LIZOL d) 11 g of DETTOL e) 26 g of DOMEX f) 28 g of HARPIC 2 a) 1.2 gms of Methanoic Staphylococcus aureus acid composition b) 9 g of SAVLON c) 10 g of LIZOL d) 11 g of DETTOL e) 27 g of DOMEX f) 29 g of HARPIC 3 a) 1.3 gms of Methanoic Escherichia coli acid composition b) 9 g of SAVLON c) 14 g of LIZOL d) 18 g of DETTOL e) 27 g of DOMEX f) 29 g of HARPIC 4 a) 1.4 gms of Methanoic Salmonella abony acid composition b) 8 g of SAVLON c) 10 g of LIZOL d) 10 g of DETTOL e) 28 g of DOMEX f) 30 g of HARPIC

(105) The following table exemplifies the effect of the compositions as identified by Sample A to Sample H of Table 1 over the pathogens as referred in Table 2.

(106) TABLE-US-00003 TABLE 3 Quantum Quantum of of known composition of disinfectant the invention PATHOGEN required to required to TESTED obtain NIL obtain NIL REMARKS UPON GROWTH GROWTH [Higher Effectiveness] Psedomonas a) 8 g of 0.8 gms of the Methanoic acid aeruginosa SAVLON Methanoic composition as b) 10 g of composition as presented by the LIZOL presented by alphabet A in c) 11 g of the alphabet Table 1 above is thus DETTOL A in Table 1 a) 10 times as d) 26 g of above was effective as DOMEX sufficient to SAVLON e) 28 g of ensure Nil b) 12.5 times as HARPIC Growth effective as LIZOL c) 13.75 times as effective as DETTOL d) 32.5 times as effective as DOMEX e) 35 times as effective as HARPIC Staphylococcus a) 9 g of 0.8 gms of the Methanoic acid aureus SAVLON Methanoic composition as b) 10 g of composition as presented by the LIZOL presented by alphabet B in c) 11 g of the alphabet Table 1 above is thus DETTOL B in Table 1 a) 11.25 times as d) 27 g of above was effective as DOMEX sufficient to SAVLON e) 29 g of ensure Nil b) 12.5 times as HARPIC Growth effective as LIZOL c) 13.75 times as effective as DETTOL d) 33.75 times as effective as DOMEX e) 38.6 times as effective as HARPIC Escherichia a) 9 g of 1 gm of the Methanoic acid coli SAVLON Methanoic composition as b) 14 g of composition as presented by the LIZOL presented by alphabet C in c) 18 g of the alphabet Table 1 above is thus DETTOL C in Table 1 a) 9 times as d) 27 g of above was effective as DOMEX sufficient to SAVLON e) 29 g of ensure Nil b) 14 times as HARPIC Growth effective as LIZOL c) 18 times as effective as DETTOL d) 27 times as effective as DOMEX e) 29 times as effective as HARPIC Salmonella a) 8 g of 1.5 gms of the Methanoic acid abony SAVLON Methanoic composition as b) 10 g of composition as presented by the LIZOL presented by alphabet D in c) 10 g of the alphabet Table 1 above is thus DETTOL D in Table 1 a) 5.3 times as d) 28 g of above was effective as DOMEX sufficient to SAVLON e) 30 g of ensure Nil b) 6.6 times as HARPIC Growth effective as LIZOL c) 6.6 times as effective as DETTOL d) 18.6 times as effective as DOMEX e) 20 times as effective as HARPIC Psedomonas a) 8 g of 1.6 gms of the Methanoic acid aeruginosa SAVLON Methanoic acid composition as b) 10 g of composition as presented by the LIZOL presented by alphabet E in c) 11 g of the alphabet Table 1 above is thus DETTOL E in Table 1 a) 5 times as d) 26 g of above was effective as DOMEX sufficient to SAVLON e) 28 g of ensure Nil b) 6.25 times as HARPIC Growth effective as LIZOL c) 6.8 times as effective as DETTOL d) 16.25 times as effective as DOMEX e) 17.5 times as effective as HARPIC Staphylococcus a) 9 g of 2.2 gms of the Methanoic acid aureus SAVLON Methanoic acid composition as b) 10 g of composition as presented by the LIZOL presented by alphabet F in c) 11 g of the alphabet Table 1 above is thus DETTOL F in Table 1 a) 4 times as d) 27 g of above was effective as DOMEX sufficient to SAVLON e) 29 g of ensure Nil b) 4.5 times as HARPIC Growth effective as LIZOL c) 5 times as effective as DETTOL d) 12.2 times as effective as DOMEX e) 13.1 times as effective as HARPIC Escherichia a) 9 g of 3.4 gms of the Methanoic acid coli SAVLON Methanoic acid composition as b) 14 g of composition as presented by the LIZOL presented by alphabet G in c) 18 g of the alphabet Table 1 above is thus DETTOL G in Table 1 a) 2.6 times as d) 27 g of above was effective as DOMEX sufficient to SAVLON e) 29 g of ensure Nil b) 4.1 times as HARPIC Growth effective as LIZOL c) 5.2 times as effective as DETTOL d) 7.9 times as effective as DOMEX e) 8.5 times as effective as HARPIC Salmonella a) 8 g of 5.4 gms of the Methanoic acid abony SAVLON Methanoic acid composition as b) 10 g of composition as presented by the LIZOL presented by alphabet H in c) 10 g of the alphabet Table 1 above is thus DETTOL H in Table 1 a) 1.4 times as d) 28 g of above was effective as DOMEX sufficient to SAVLON e) 30 g of ensure Nil b) 1.8 times as HARPIC Growth effective as LIZOL c) 1.8 times as effective as DETTOL d) 5.1 times as effective as DOMEX e) 5.5 times as effective as HARPIC

(107) It may be noted that the composition of the invention can be used without admixing with any other composition. It may further be noted that the composition of the invention can be admixed with any other known composition to obtain the desired combined effects of the composition mixtures. It may further be noted that in such mixing compositions the mixing ratio does not place much role because the skilled person in the artisan will be capable of doing so.