Technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation

Abstract

The present disclosure discloses a technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation. The technical method comprises the following steps: extraction of hair follicles; separation of hair follicles; in-vitro culture of hair follicle melanocyte stem cells; inactivation of hair follicles; and transplantation of hair follicle melanocyte stem cells. According to the present disclosure, outer root sheaths of hair follicles containing the hair follicle melanocyte stem cells are obtained through a precise extraction and separation method. Through in-vitro separation and culture and inactivation of hair follicles, a situation that vitiligo only turns black without hairs after surgery is achieved. Through precise transplantation, the original color of the punctate multi-hair follicle orifice can be restored, thereby achieving the purpose of rapidly removing white patches.

Claims

1. An in-vitro preparation method of hair follicle melanocyte stem cells comprising the following steps: step S1: extracting one unit of hair follicles by using a vitiligo hair follicle extractor, wherein colored single-hair follicles or double-hair follicles are firstly selected, and the hair follicles with white hairs are not selected and meanwhile, 5-60 ml of blood are drawn from patients and then centrifugally separated to obtain required serum for in-vitro culture; step S2, separating a single complete outer hair root sheath with epidermis and containing hair follicle melanocyte stem cells from the hair follicles under a 2-15 magnifying glass or electron microscope, wherein the outer single complete hair root sheath contains all the melanocyte stem cells and mature melanocytes extracted from the hair follicles; step S3, putting the single-hair follicle of the complete outer hair root sheath containing the hair follicle melanocyte stem cells into a hair follicle storage ice box for culture, to enhance the activity of the hair follicle melanocyte stem cells and promote the differentiation and proliferation of the hair follicle melanocyte stem cells and transformation into the mature melanocytes, and culturing the mature melanocytes into a special culture solution at 0-4 C. for 60 min, and irradiating the cultured melanocytes for 5-50 s with 308NM excimer laser for later use wherein, the main ingredients of the special culture solution are psoralea corylifolia injection, dexamethasone sodium phosphate injection, autologous serum, low-molecular-weight heparin sodium and normal saline; and step S4, inactivating the hair follicles with the help of a dermoscope and a novel vitiligo hair follicle inactivation needle.

2. The in-vitro preparation method of the hair follicle melanocyte stem cells according to claim 1, wherein in the step S1 extraction of the hair follicles comprises extracting one unit of the hair follicles by using a vitiligo hair follicle extractor, wherein colored single-hair follicles or double-hair follicles are firstly selected to facilitate separation, the hair follicles with the white hairs are not selected, and meanwhile 5-60 ml of blood are drawn from patients and then centrifugally separated to obtain required serum for in-vitro culture of the hair follicle melanocyte stem cells.

3. The in-vitro preparation method of the hair follicle melanocyte stem cells according to claim 2, wherein in the step S2 the separation of the hair follicles, specifically; comprises separating a single complete outer hair root sheath with epidermis and containing the hair follicle melanocyte stem cells from the hair follicles under a 2-15 magnifying glass or electron microscope to extend the movement pathway of the hair follicle melanocyte stem cells and increase number of the mature melanocytes to enter the peripheries of more hair follicle orifices.

4. The in-vitro preparation method of the hair follicle melanocyte stem cells according to claim 1, wherein the step S4 in the in-vitro preparation method of hair follicle melanocyte stem cells comprises: inactivating the hair follicles cultured with the special culture solution, by first, holding hair follicle tails with hair follicle extraction forceps, then inactivating the hair follicles with a novel vitiligo hair follicle inactivation needle under the microscope, the inactivation being performed by aligning an enlarged hair nipple and stabbing into the middle of the hair nipple until a snap sound is heard and the liquid runs up, so that the hair follicles are not able to enter the next hair cycle thereby putting the hair follicle in a vegetative state, so as to inactivate the hair follicles and realize that vitiligo only turns black without hair after surgery.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) Accompanying drawings are intended to further understand the present disclosure and constitute one part of the specification, and used for explaining the present disclosure together with specific embodiments of the present disclosure, but not limited thereto; in which:

(2) FIG. 1 is a flowchart of a technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation according to the present disclosure.

DETAILED DESCRIPTION OF THE INVENTION

(3) Next, preferred embodiments of the present disclosure will be described in detail in combination with drawings. It should be understood that the preferred embodiments described herein are only for illustrating and explaining the present disclosure but not limiting thereto.

(4) The technical key points of the present disclosure are as follows: 1, in-vitro culture is performed on the obtained hair follicle melanocyte stem cells by using the in-vitro culture technology of the hair follicle melanocyte stem cells, specifically, main components psoralea corylifolia injection, dexamethasone sodium phosphate injection, autologous serum and normal saline are cultured for 60 min at 0-4 C., and then irradiated for 5-50 s by using 308NM excimer laser for later use; 2, the hair follicles prior to transplantation are inactivated by using the hair follicle inactivation technology with the help of the dermoscope and the hair follicle inactivation needle, and the method is as follows: first, hair follicle tails are held with hair follicle extraction forceps, the hair nipple was stabbed until hear the snap sound is heard and the liquid runs up, so that the hair follicles are inactivated and vitiligo only turns black without hairs after surgery.

Example 1

(5) As shown in FIG. 1, the present disclosure provides a technical solution: a technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation comprises the following steps: step S1: extraction of hair follicles, specifically, one unit of hair follicles were extracted by using a vitiligo hair follicle extractor (Chinese patent certificate No.: 11005086) invented by this company, wherein colored single hair follicles were firstly selected, hair follicles with white hairs were not selected, and meanwhile 5-60 ml of blood from patients were drawn and then centrifugally separated to obtain required serum for later use; step S2, separation of hair follicles, specifically, a single complete outer hair root sheath containing hair follicle melanocyte stem cells were separated from the hair follicle unit under the 2-15 magnifying glass or electron microscope; step S3, in-vitro culture of hair follicle melanocyte stem cells, specifically, the extracted and separated single hair follicle were cultured in a special culture solution for later use, so as to enhance the activity of the hair follicle melanocyte stem cells and transformation into the mature melanocytes through culture; step S4, inactivation of hair follicles, specifically, hair follicles prior to transplantation were inactivated by means of a dermoscope and a hair follicle inactivation needle; and step S5, transplantation of hair follicle melanocyte stem cells, specifically, the outer hair follicle sheath containing the hair follicle melanocyte stem cells was accurately implanted at the specific level of vitiligo by using a specially made implant needle after inactivation of hair follicles, so as to promote differentiation and proliferation of the melanocyte cells and formation of tissues and realize that the color of the punctate multi-hair follicle orifice can be restored, thereby achieving the purpose of rapidly removing white patches.

(6) In this example, the specific method of step S1 was as follows: one unit of hair follicles was extracted by using a vitiligo hair follicle extractor invented by this company, wherein colored single-hair follicles or double-hair follicles were firstly selected, and hair follicles with white hairs were not selected; and meanwhile 5-60 ml of blood were drawn from patients and then centrifugally separated to obtain required serum for later use.

(7) In this example, in the step S2, the complete outer hair root sheath of the melanocyte stem cell containing epidermis was separated from the hair follicle unit.

(8) In this example, in the step S3, in-vitro culture was performed on the hair follicle melanocyte stem cells, specifically, the single hair follicles were put into a special culture solution for culture at 0-4 C. for 60 min, and then were irradiated for 5-50 s with 308NM excimer laser for later use, wherein the main components of the special culture solution were psoralea corylifolia injection, dexamethasone sodium phosphate injection, autologous serum and normal saline, the irradiated hair follicles were cultured in the special culture solution for later use, so that the enhancement of the activity of the melanocyte stem cells and transformation into the mature melanocyte stem cells were achieved through culture.

(9) In this example, in the step S4, hair follicles cultured in the special culture solution were inactivated, specifically, hair follicle tails were first held with hair follicle extraction forceps, the hair follicles were inactivated with a novel vitiligo hair follicle inactivation needle (Chinese patent certificate No.: 10979242) under the microscope, and the hair nipple was stabbed until hear the snap sound was heard and the liquid runs up, so as to inactivate the hair follicles and realize that vitiligo only turned lack without hairs after surgery.

(10) In this example, in the step S5, the outer hair follicle sheath containing the hair follicle melanocyte stem cells was accurately implanted at the special level of vitiligo by using a implantation needed for treating vitiligo (Chinese patent certificate No.: 11440332), so as to promote the proliferation and differentiation of melanocyte stem cells and formation of tissues and realize that the color of the punctate multi-hair follicle orifice was restored, thereby achieving the purpose of rapidly removing white patches.

Example 2

(11) As shown in FIG. 1, the present disclosure provides a technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation. Through the technical method in example 1, the hair follicle melanocyte stem cells were transplanted at the special level of vitiligo so as to promote the proliferation and differentiation of melanocyte stem cells and formation of tissues and realize that the color of the punctate multi-hair follicle orifice was restored, thereby achieving the purpose of rapidly removing white patches.

(12) In this example, through the transplantation technology of the hair follicle melanocyte stem cells, the curing rate of vitiligo reached 90% or more.

(13) The technical difficulties and surgical procedures of the present disclosure:

(14) Technical difficulties: survival rate refers to a fact that whether transplanted melanocyte stem cells can be directly survived, which is related to subsequent treatment effects. Autologous melanocyte stem cells obtained by extraction have the characteristics that they are not affected by internal secretion and hormone of an organism, and cannot be affected by inpatient areas after being cultured and activated to be implanted in focal areas of vitiligo to ensure a postoperative curative effect.

(15) surgical procedures: 1, one unit of hair follicle is extracted by using the vitiligo hair follicle extractor (Chinese patent certificate No.: 11005086), wherein hair follicle of the hair follicle melanocyte stem cell containing epidermis is extracted; the complete hair follicle outer root sheath of the hair follicle melanocyte stem cell containing epidermis is obtained through a special separation method; the hair follicles after extraction and separation are cultured into the special culture solution for 60 min at 0-4 C. and then irradiated for 5-50 s with 308NM excimer laser for later use, subsequently, the extracted hair follicles are inactivated with the help of a magnifying glass or microscope and a hair follicle inactivation needle to achieve a fact that vitiligo only turned black without hair after surgery; 2, implantation is a key point associated with survival of melanocyte stem cells, if the planting is too shallow, the survival rate is low, and the effect is not good, while if the implantation is too deep, the melanocyte stem cells cannot move to the hair follicle mouth of the epidermis of vitiligo along the outer hair root sheath to cause a reduced curative effect, and therefore the punctate multi-hair follicle orifice is re-colored as long as the melanocyte stem cells are accurately implanted at the special level of vitiligo.

(16) The principle of the present disclosure: with the help of a WOOD lamp and an accurate dermoscopy imaging data report, the melanin loss degree and cell activity of patients are analyzed. The hair follicle melanocyte stem cells are extracted with a patented technology, the hair follicles are inactivated using the inactivation needle under the microscope through special culture and separation, and then the inactivated hair follicles are accurately implanted at the special level of vitiligo by using the specially made implantation needle so as to promote the proliferation and differentiation of melanocytes and formation of tissues and realize that the color of the multi-hair follicle orifice is restored, thereby achieving the purpose of rapidly removing white patches. This technology can rebuild the patient's autoimmune system in the vitiligo area and solves the problem of melanocyte inactivation, so that the treatment of vitiligo enters the era of minimally invasive surgery.

(17) The above descriptions are only preferred embodiments of the present disclosure but not limit the present disclosure. Although the present disclosure has been illustrated in detail with reference to the above-mentioned examples, those skilled in the art still can make modification on the technical solution described in each example, or equivalent substitutions to parts of technical features. Any modifications, equivalent substitutions and improvements made within the spirit and principle of the present disclosure should be included within the protective scope of the present disclosure.