COMPOSITIONS COMPRISING CHOLINE AND OMEGA-3 FATTY ACID TO NUTRITIONALLY ENHANCE FOOD PRODUCTS

Abstract

Provided herein is a composition that includes choline or choline salt and omega-3 fatty acid for nutritionally enhancing a food product, as well as the resultant food products. The food product when nutritionally enhanced with the composition has an acceptable taste or acceptable odor or the food product has a peroxide value of less than 50 mEq. Methods of preparing the composition and food products are also are provided. As seen in the accompanying figure the discoveries outlined herein have resulted in an unprecedented level of organoleptic stability over time, with no significant change to key sensory parameters typically seen in products that contain high levels of choline and omega-3 fatty acids.

Claims

1.-55. (canceled)

56. A nutritionally enhanced food composition that is ready for consumption and is fortified with a form of choline that provides between about 0.75 mg/g and about 5 mg/g free choline and between about 0.25 mg/g and about 5 mg/g omega-3 fatty acid, wherein the nutritionally enhanced food composition is a fruit or vegetable preparation, the omega-3 fatty acid comprises docosahexaenoic acid (DHA) in combination with alpha-lipoic acid (ALA) or eicosapentaenoic acid (EPA), and wherein the nutritionally enhanced food composition can be stored without refrigeration or freezing for at least 4 weeks.

57. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition further comprises one or more antioxidants.

58. The nutritionally enhanced food composition of claim 57, wherein each antioxidant of the one or more antioxidants is selected from the group consisting of tocopherols, rosemary extract, and vitamin C.

59. The nutritionally enhanced food composition of claim 56, wherein the form of the choline provides between about 0.75 mg/g and about 2 mg/g of the free choline.

60. The nutritionally enhanced food composition of claim 56, wherein the form of form of the choline is a choline salt or phosphotidyl choline.

61. The nutritionally enhanced food composition of claim 60, wherein the form of the choline is the choline salt, and wherein the choline salt is selected from the group consisting of choline acetate, choline bicarbonate, choline chloride, choline citrate, choline bitartrate, and choline lactate.

62. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition is a fruit or vegetable preparation comprising no added protein.

63. The nutritionally enhanced food composition of claim 56, wherein the omega-3 fatty acid is encapsulated.

64. The nutritionally enhanced food composition of claim 56, wherein the omega-3 fatty acid comprises the DHA in combination with the EPA.

65. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition comprises between about 0.25 mg/g and about 3 mg/g of the omega-3 fatty acid.

66. The nutritionally enhanced food composition of claim 56, wherein a peroxide value of the nutritionally enhanced food composition is less than about 50 mEq, less than about 40 mEq, less than about 35 mEq, less than about 30 mEq, less than about 25 mEq, less than about 20 mEq, less than about 15 mEq, less than about 10 mEq, or less than about 5 mEq.

67. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition comprises a probiotic or an active culture, and wherein the probiotic or the active culture comprises Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, or Bacillus.

68. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition further comprises a source of iron.

69. (canceled)

70. The nutritionally enhanced food composition of claim 83, wherein the isolated protein is selected from the group consisting of whole milk protein, casein, and whey, or the isolated protein is a vegetable protein selected from the group consisting of soy protein, pea protein, cottonseed protein, corn protein, and wheat protein.

71. A method of reducing an oxidation of an omega-3 fatty acid in a nutritionally enhanced food composition that is ready for consumption, the method comprising: fortifying the nutritionally enhanced food composition with a choline salt that provides between about 0.75 mg/g and about 5 mg/g of free choline and between about 0.25 mg/g and about 5 mg/g of the omega-3 fatty acid, wherein the nutritionally enhanced food composition is a fruit or vegetable preparation, the omega-3 fatty acid comprising DHA in combination with ALA or EPA, and wherein the nutritionally enhanced food composition can be stored without refrigeration or freezing for at least 4 weeks.

72. A method of preparing a nutritionally enhanced food composition that is ready for consumption, the method comprising: providing a fruit or vegetable preparation less; and combining a choline salt that provides between about 0.75 mg/g and about 5 mg/g of free choline and between about 0.25 mg/g and about 5 mg/g of an omega-3 fatty acid with the fruit or vegetable preparation to prepare the nutritionally enhanced food composition, the omega-3 fatty acid comprises docosahexaenoic acid (DHA) in combination with alpha-lipoic acid (ALA) or eicosapentaenoic acid (EPA), and wherein the nutritionally enhanced food composition can be stored without refrigeration or freezing for at least 4 weeks.

73. The method of claim 72, wherein a peroxide value of the nutritionally enhanced food composition is less than about 50 mEq, less than about 40 mEq, less than about 35 mEq, less than about 30 mEq, less than about 25 mEq, less than about 20 mEq, less than about 15 mEq, less than about 10 mEq, or less than about 5 mEq.

74. The method of claim 72, wherein the choline salt is combined with the at least one edible food ingredient before the omega-3-fatty acid is combined with the at least one edible food ingredient, or the omega-3-fatty acid is combined with the at least one edible food ingredient before the choline salt is combined with the at least one edible food ingredient.

75. The method of claim 72, wherein the choline salt and the omega-3-fatty acid are combined simultaneously with the at least one edible food ingredient.

76. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition further comprises protein or an isolated protein.

77. The nutritionally enhanced food composition of claim 76, wherein the nutritionally enhanced food composition comprises 1 g of protein or less.

78. The nutritionally enhanced food composition of claim 76, wherein the nutritionally enhanced food composition comprises at least 25 mg/g protein.

79. The nutritionally enhanced food composition of claim 76 wherein the nutritionally enhanced food composition comprises 15 mg/g to about 55 mg/g of protein.

80. The nutritionally enhanced food composition of claim 76, wherein the nutritionally enhanced food composition comprises 25-200 mg/g protein, 25-180 mg/g protein, 25-170 mg/g protein, 25-150 mg/g protein, 25-125 mg/g protein, 25-120 mg/g protein, 25-110 mg/g protein, 25-100 mg/g protein, 25-90 mg/g protein, 25-80 mg/g protein, 25-70 mg/g protein, 25-60 mg/g protein, or 25-50 mg/g protein.

81. The nutritionally enhanced food composition of claim 76, wherein the nutritionally enhanced food composition comprises an amount of protein by mass between 0.05% to 5%, between 0.05% to 4%, between 0.05% to 3%, between 0.1% to 5%, between 0.1% to 4%, between 0.1% to 3%, between 10 0.2% to 5%, between 0.2% to 4%, between 0.2% to 3%, between 0.5% to 5%, between 0.5% to 4%, between 0.5% to 3%, between 0.75% to 5%, between 0.75% to 4%, between 0.75% to 3%, between 1% to 5%, between 1% to 4%, between 1% to 3% or between 1% to 2%.

82. The nutritionally enhanced food composition of claim 76, wherein the nutritionally enhanced food composition comprises between 10 and 25 grams of protein per serving.

83. The nutritionally enhanced food composition of claim 56, wherein the nutritionally enhanced food composition further comprises an isolated protein.

84. The nutritionally enhanced food composition of claim 83, wherein the isolated protein is whole milk protein, casein, and/or whey.

85. The nutritionally enhanced food composition of claim 83, wherein the isolated protein is a vegetable protein selected from soy protein, pea protein, cottonseed protein, corn protein, and/or wheat protein

86. The nutritionally enhanced food composition of claim 83, wherein the isolated protein is isolated plant protein, pulse protein, nut protein, algal protein, dairy protein and/or mycoprotein.

87. The nutritionally enhanced food composition of claim 86, wherein the isolated plant protein is barley protein, oat protein, rice protein, wheat protein, and/or potato protein.

88. The nutritionally enhanced food composition of claim 86, wherein the isolated pulse protein is selected from common bean protein (Phaseolus vulgaris), chickepea protein, fava bean protein, lentil protein, lima bean protein, mung bean protein, peanut protein, and/or soy protein.

89. The nutritionally enhanced food composition of claim 86, wherein the isolated nut protein is selected from almond protein, cashew protein, chestnut protein, hazelnut protein, macadamia nut protein, pecan protein, pistachio protein, and/or walnut protein.

90. The nutritionally enhanced food composition of claim 86, wherein the isolated dairy protein is selected from whey protein and casein.

91. The nutritionally enhanced food composition of claim 56, further comprising a dairy product.

92. A nutritionally enhanced food composition that is ready for consumption and is fortified with a form of choline that provides between about 0.75 mg/g and about 5 mg/g free choline and between about 0.25 mg/g and about 5 mg/g omega-3 fatty acid, wherein the nutritionally enhanced food composition is a dairy product, the omega-3 fatty acid comprises docosahexaenoic acid (DHA) in combination with alpha-lipoic acid (ALA) or eicosapentaenoic acid (EPA), and wherein the nutritionally enhanced food composition can be stored without refrigeration or freezing for at least 4 weeks.

93. The nutritionally enhanced food composition of claim 92, wherein the dairy product is whole milk, reduced fat milk, flavored milk, flavored milk beverage. meal replacement beverage, protein shake, yogurt based smoothie, yogurt, cheese, cheese product, liquid creamer, powdered creamer, probiotic shot, pudding, desert dip, dressing, sauce, or condiment.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0150] FIG. 1 shows the development of fishy flavor in a yogurt drink stored in a lighted dairy case. (See Example 1.)

[0151] FIG. 2 shows a spider chart of the flavor characteristics of a yogurt drink stored for 14 days in a lighted dairy case. (See Example 1.)

[0152] FIG. 3 shows a spider chart of the flavor characteristics of a yogurt drink stored for 28 days in a lighted dairy case. (See Example 1.)

[0153] FIG. 4 shows a spider chart of the flavor characteristics of a yogurt drink stored for 42 days in a lighted dairy case. (See Example 1.)

[0154] FIG. 5 shows a spider chart of the flavor characteristics of a yogurt drink stored for 56 days in a lighted dairy case. (See Example 1.)

[0155] FIG. 6 shows a spider plot of sensory profile of drinkable yogurts across time. Sweet taste is not indicated on graph so that other differences can be visualized. (See Example 10.)

[0156] FIG. 7 shows a spider plot of sensory profile of yogurts across time. Sweet taste is not indicated on graph so that other differences can be visualized. (See Example 10.)

[0157] FIG. 8 shows a sensory profile of cherry vanilla yogurts across time. Sweet taste is not indicated on graph so that other differences can be visualized. (See Example 11.)

[0158] FIG. 9 shows a sensory profile of strawberry banana yogurts across time. Sweet taste is not indicated on graph so that other differences can be visualized. (See Example 11.)

DETAILED DESCRIPTION

Definitions

[0159] Unless defined otherwise below, all technical and scientific terms used herein have the meaning commonly understood by a person skilled in the art to which this invention belongs.

[0160] Acceptable appearance, acceptable aroma, acceptable odor, acceptable taste, acceptable flavor, or acceptable texture is an appearance, aroma, odor, taste, flavor, or texture that is acceptable to a sensory panel. The acceptable appearance, aroma, odor, taste, flavor, taste or texture can be compared to a reference product. A sensory panel can be a trained sensory panel or a non-trained sensory panel.

[0161] Baked good means a food item that is prepared by using an oven and usually contains a leavening agent. Baked goods include, but are not limited to brownies, cookies, pies, cakes and pastries.

[0162] Bread means a food item that contains flour, liquid, and usually a leavening agent. Breads are usually prepared by baking in an oven, although other methods of cooking are also acceptable. The leavening agent can be chemical or organic/biological in nature. Typically, the organic leavening agent is yeast. In the case where the leavening agent is chemical in nature (such as baking powder and/or baking soda), these food products are referred to as quick breads. Crackers and other cracker-like products are examples of breads that do not contain a leavening agent.

[0163] Choline is an essential nutrient that is critical in many biological processes including brain development. Choline is 2-Hydroxy-N,N,N-trimethylethan-1-aminium. Choline salt is the salt form of choline. Choline salts include choline acetate, choline bicarbonate, choline chloride, choline citrate, choline bitartrate, choline lactate, and other choline salts. Choline also exists in the form of phoshotidylcholine. Phosphotidylcholines are a class of phospholipids that incorporate choline as a headgroup. Unless otherwise indicated, the term choline refers to any form of choline that can provide choline to a food product.

[0164] Canned product is a product that is packaged in a can that has a long shelf life. A retorted product is a product that is packaged in a pouch that has a long shelf life.

[0165] Conventional food product means a food composition or product intended for consumption, e.g., by a human, that is not nutritionally enhanced with a choline salt, omega-3 fatty acid, and optionally the conventional food product is not nutritionally enhanced with isolated protein.

[0166] Cooked product means a food that has been heated, e.g., in an oven, for a period of time.

[0167] Dairy product is any product that comprises a dairy ingredient. Dairy products include milk, yogurt, drinkable yogurt, cheese, cream, sour cream, cottage cheese and other products that contain ingredients derived from milk.

[0168] Edible ingredient means any substance or composition that is fit to be eaten. Edible ingredients include, without limitation, grains, fruits, vegetables, proteins, herbs, spices, carbohydrates, sugar, and fats as well as substances that are isolated therefrom.

[0169] Drink as used herein is a food that is drinkable and/or gulpable. For example, a drinkable yogurt product is a drink.

[0170] The term ingredient as used herein means ingredients used in foods and/or food compositions or products, including fruit or vegetable prepartions. Ingredient includes, without limitation, preservatives, flavorants, food additives, food coloring, sugar substitutes and other ingredients found in various foods.

[0171] Fat means a lipid or mixture of lipids that is generally liquid or solid at ordinary room temperatures and pressures. Fat includes, without limitation, lard and butter.

[0172] Fortified food composition, nutritionally enhanced food composition, nutritionally enhanced food product and Finished food product mean a food composition that is ready for packaging, use, or consumption. For example, a nutritionally enhanced food composition may have been cooked or the ingredients comprising the finished food product may have been mixed or otherwise integrated with one another.

[0173] Nutritionally enhanced food product, Finished food product, and fortified food product mean a food that is ready for packaging, use, or consumption. For example, a nutritionally enhanced food product may have been cooked or the ingredients comprising the finished food product may have been mixed or otherwise integrated with one another.

[0174] Fresh product is a product that is not frozen, canned or otherwise preserved to have a long shelf life.

[0175] Food, food composition, food product and foodstuff mean any composition intended to be or expected to be ingested by humans as a source of nutrition and/or calories. Food compositions or products can be composed primarily of carbohydrates, fats, water and/or proteins and make up substantially a person's entire daily caloric intake. A fruit preparation or vegetable preparation is a food, food composition, food product or foodstuff. A food composition, food product, nutritionally enhanced food composition, or nutritionally enhanced food product is typically not encapsulated or in tablet form.

[0176] Fortifying or nutritionally enhancing means the addition of nutrients or other chemicals to increase the nutritional content of food food products. Fortifying and nutritionally enhancing is used interchangeably herein.

[0177] Frozen product is a product that is stored, transported, and/or sold in freezers at temperatures less than 0 C. and a solid at freezer temperatures. Frozen products are often consumed when the temperature of the product is less than 0 C.

[0178] Fruit preparation or vegetable preparation can refer to a puree of a fruit or vegetable, but can also include preparations that include small, cut pieces of fruit or vegetable. The addition of the small, cut fruit or vegetable pieces to a puree produces fruit or vegetable preparations that can be chewed when consumed. The mouth feel provided by chewing the small, cut fruit pieces or vegetable pieces is desirable in certain applications.

[0179] Gelatin is a food ingredient derived from collagen. Gelatin is prepared from collagen by exposure to acid, base, or heat. The exposure to acid, base, or heat denatures the collagen polypeptides and forms reversible gels.

[0180] High temperature, short time or HTST also known as flash pasteurization is a technique of pasteurizing foods to produce foods that are minimally exposed to heat and pasteurize the food. HTST treatment regime can be used to produce a product with acceptable biocontaminants. For example, the temperature of the treatment may be a temperature of from 65 C. to 100 C., from 65 C. to 95 C., from 65 C. to 90 C., from 65 C. to 85 C., from 65 C. to 80 C., from 65 C. to 75 C., from 65 C. to 70 C., from 70 C. to 100 C., from 70 C. to 95 C., from 0 C. to 90 C., from 70 C. to 85 C., from 70 C. to 85 C., from 70 C. to 80 C. or from 70 C. to 75 C., for a period of from 1 second to 180 seconds, from 1 second to 120 seconds, from 1 second to 90 seconds, from 1 second to 60 seconds, from 1 second to 30 seconds, from 5 seconds to 120 seconds, from 5 second to 90 seconds, from 5 seconds to 60 seconds, from 5 seconds to 30 seconds, from 10 seconds to 120 seconds, from 10 seconds to 90 seconds, from 10 seconds to 60 seconds, from 10 seconds to 30 seconds or from 10 seconds to 20 seconds.

[0181] Ingredient comprising protein is an ingredient that comprises protein. Ingredients that comprise protein include milk, milk products, yogurt, frozen yogurt butter, cream, ice cream, soft serve ice cream, sour cream, cheese, grain, flour, legumes, vegetables, fruit, products derived from vegetable or fruit, such as apple sauce, nuts, nut butters, meat products, and other ingredients that are used to prepare food products or consumed directly.

[0182] Isolated protein means a polypeptide that is separated from the original source of the polypeptide. Milk proteins are polypeptides isolated from milk. Casein and whey are examples of isolated mild proteins. Concentrated milk protein (MPC) is another milk protein. Similarly, soy proteins are polypeptides isolated from soy. A protein concentrate is an isolated protein.

[0183] Lipid means any of a class of molecules that are soluble in nonpolar solvents or polar organic solvents and relatively or completely insoluble in water. Lipid molecules have these properties, because they are largely composed of long hydrocarbon tails that are hydrophobic in nature. Examples of lipids include fatty acids (saturated and unsaturated); glycerides or glycerolipids (such as monoglycerides, diglycerides, triglycerides or neutral fats, and phosphoglycerides or glycerophospholipids); and nonglycerides (sphingolipids, tocopherols, tocotrienols, sterol lipids including cholesterol and steroid hormones, prenol lipids including terpenoids, fatty alcohols, waxes, and polyketides).

[0184] Non-photo protective packaging material is packaging material that does not provide protection against UV and visible light.

[0185] Nutritional supplement means a composition intended to supplement the diet by providing specific nutrients as opposed to bulk calories. A nutritional supplement may contain any one or more of the following ingredients: a vitamin, a mineral, an herb, an amino acid, an essential fatty acid, and other substances. Nutritional supplements are typically tableted or encapsulated. A single tableted or encapsulated nutritional supplement is typically ingested at a level no greater than 15 grams per day. A nutritionally enhanced food composition, food, food composition, food product or food stuff is not a nutritional supplement.

[0186] Oil means any triacylglyceride (or triglyceride oil), produced by organisms, including microalgae, other plants, and/or animals. Oil, as distinguished from fat, refers, unless otherwise indicated, to lipids that are generally liquid at ordinary room temperatures and pressures. However, coconut oil is typically solid at room temp, as are some palm oils and palm kernel oils. For example, oil includes vegetable or seed oils derived from plants, including without limitation, an oil derived from soy, rapeseed, canola, palm, palm kernel, coconut, corn, olive, sunflower, cotton seed, cuphea, peanut, camelina sativa, mustard seed, cashew nut, oats, lupine, kenaf, calendula, hemp, coffee, linseed, hazelnut, euphorbia, pumpkin seed, coriander, camelina, sesame, safflower, rice, tung oil tree, cocoa, copra, pium poppy, castor beans, pecan, jojoba, jatropha, macadamia, Brazil nuts, and avocado, as well as combinations thereof. Animal fats and oils includes butterfat, cream, ghee, lard, tallow and other edible fats and oils derived from animals.

[0187] Omega-3 fatty acids also called -3 fatty acids or n-3 fatty acids are polyunsaturated fatty acids (PUFA) that contain a carbon-carbon double bond at the -3 position of the carbon chain of the fatty acid. Three common types of fatty acids are alpha-linolenic acid (ALA), found in plant oils, and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) found in marine oils. Marine algae are sources of DHA and EPA and plant oils such as flax seed oil and walnut oil are common sources of ALA.

[0188] Peroxide value is a measure of the rancidity of unsaturated fats and oils. Peroxide value is defined as the amount of peroxide oxygen arising from degradation of unsaturated fats per kilogram oil. Peroxide values are typically expressed as milliequivalents.

[0189] Polysaccharide is a polymeric carbohydrate molecule. Polysaccharides can be modified or unmodified.

[0190] Probiotic, or active culture are live microorganisms that provide health benefits when consumed. Yogurt, kombucha, miso, sauerkraut, kimchi are foods that are known to provide probiotics or active cultures when consumed.

[0191] Protein or polypeptide as used herein is a chain of amino acid monomers linked together amide bonds or peptide bonds. Protein and polypeptide comprise at least two amino acid monomers an may include hundreds or thousands of amino acid monomers.

[0192] Puree is a fruit or vegetable that has been pureed, mashed, squashed, chopped, blended, macerated, juiced or otherwise processed to a liquid or pourable form. The fruit or vegetable that has been pureed, mashed, squashed, chopped, blended, macerated, juiced or otherwise processed to a liquid or pourable form can be in concentrated form. For example, the term puree includes concentrated fruit juice or concentrated vegetable juice. The puree can also include small, cut pieces of the fruit or vegetable.

[0193] Refrigerated or refrigerated storage mean that a food composition or product (including a fruit or vegetable preparation) is stored in a temperature controlled environment that maintains a temperature that is below room temperature. Typically, refrigerated temperatures range from 1 C. to 10 C.

[0194] Shelf stable product is a product that can be safely stored at room temperature in a sealed container for an extended period of time.

[0195] Suitable for human consumption means a composition can be consumed by humans as dietary intake without ill health effects and can provide significant caloric intake due to uptake of digested material in the gastrointestinal tract.

[0196] Uncooked product means a composition that has not been subjected to heating but may include one or more components previously subjected to heating.

[0197] W/W or w/w, in reference to proportions by mass, means the ratio of the mass (weight) of one substance in a composition to the total mass of the composition (e.g., fruit or vegetable composition). For example, a nutritionally enhanced food composition or product that comprises 5% w/w omega-3 fatty acid means that 5% of the nutritionally enhanced food composition's or product's mass is omega-3 fatty acid and the remainder of the weight of the composition s composed of other ingredients. For example, for a food composition or product that is 5% w/w omega-3 fatty acid, 100 grams of a nutritionally enhanced food composition or product has 5 grams of the omega-3 fatty acid. Similarly, a food composition or product that is 2.5% w/w choline (or choline salt), 100 grams of a nutritionally enhanced food composition or product has 2.5 grams of choline (or choline salt).

Compositions Including Choline and Omega-3 Fatty Acid to Nutritionally Enhance Food Products and Nutritionally Enhanced Food Products

[0198] A composition including choline and omega-3 fatty acid for nutritionally enhancing a food product is provided. When nutritionally enhanced with the composition, the food product has an acceptable taste or acceptable odor, as determined by a sensory panel, or the food product has a peroxide value of less than 50 mEq.

[0199] The oxidation of polyunsaturated fatty acids, such as omega-3 fatty acids, in foods produce food products with unacceptable taste or unacceptable odor. It has been surprisingly and unexpectedly discovered that composition having high levels of choline or choline salt, and high levels of omega-3 fatty acids when added to food products produce food product with acceptable taste, acceptable odor or produces food products with peroxide values of less than 50 mEq.

[0200] In various embodiments, the peroxide value of the food product when nutritionally enhanced with the composition provided herein is less than 50 mEq, less than 40 mEq, less than 35 mEq, less than 30 mEq, less than 25 mEq, less than 20 mEq, less than 15 mEq, less than 10 mEq, or less than 5 mEq. Alternatively, the peroxide value of the food product is between 1-50 mEq, 1-45 mEq, 1-40 mEq, 1-35 mEq, 1-30 mEq, 1-25 mEq, 1-24 mEq, 1-23 mEq, 1-22 mEq, 1-21 mEq, 1-20 mEq, 1-17.5 mEq, 1-15 mEq, 1-12.5 mEq, and 1-10 mEq. Peroxide values are measured in commercially available machines and can be performed according to AOCS Official Method Cd 8-53, ISO/IEC 17025, or other known methods.

[0201] In one embodiment, the composition is added to the food product in a sufficient amount to produce a food product that comprises at least 0.25 mg choline salt per gram of food product (mg/g) and at least 0.25 mg omega-3 fatty acid per gram of food product (mg/g). Unless otherwise indicated, the unit mg/g refers to milligrams of constituent (e.g., choline salt or omega-3 fatty acid) per gram of food product. Accordingly, the phrase at least 0.25 mg/g choline salt means at least 0.25 mg choline salt per gram of food product, and the phrase at least 0.25 mg/g omega-3 fatty acid means at least 0.25 mg omega-3 fatty acid per gram of food product.

[0202] In certain embodiments, the food product comprises at least 25 mg protein per gram of food product before or after addition of the composition to the food product. In some embodiments, isolated protein, which can be included in the composition, is added to the food product to achieve a protein level of at least 25 mg protein per gram of food product (also expressed as 25 mg/g protein).

[0203] In some embodiments, the level of oxidation of the omega-3 fatty acids present in the food product is decreased as compared to a food that is not nutritionally enhanced with the composition.

Choline

[0204] Any suitable source of choline can be used in the compositions, food products, and methods described herein. In some embodiments, the nutritionally enhanced food product when nutritionally enhanced with the composition provides a food product that includes at least 0.25 mg choline or choline salt per gram of food product. In various embodiments, the food product is nutritionally enhanced with the composition provided herein to provide choline or choline salt at a concentration of between 0.25 mg/g and 5 mg/g, between 0.25 mg/g and 4 mg/g, between 0.25 mg/g and 3.5 mg/g, between 0.25 mg/g and 3 mg/g, between 0.25 mg/g and 2.5 mg/g, between 0.25 mg/g and 2 mg/g, between 0.25 mg/g and 1.75 mg/g, between 0.25 mg/g and 1.5 mg/g, between 0.25 mg/g and 1.25 mg/g, and between 0.25 mg/g and 1 mg/g. The amount of choline salt in the composition is thus sufficient to provide the desired concentration of choline salt to the food product. As those of skill in art readily appreciate, this amount will vary depending on the relative amounts of the choline- or choline salt-containing composition and the food product that are combined.

[0205] The choline salt of the composition taught herein can be selected from the group consisting of choline acetate, choline bicarbonate, choline chloride, choline citrate, choline bitartrate, and choline lactate.

[0206] In some embodiments, phosphotidylcholine and/or free-base choline is used, instead of or in addition to a choline salt, to supply choline to the nutritionally enhanced food. Suitable concentrations of different forms of choline can be determined based on the guidance provided herein. For example, as those of skill in the art know, choline chloride is 74% choline by weight, whereas phosphotidylcholine is only 13% by weight. In general, the amount of, e.g., phosphotidylcholine used should supply choline in the amounts given above.

Omega-3 Fatty Acid

[0207] The nutritionally enhanced food product when nutritionally enhanced with the composition provides a food product that comprises at least 0.25 mg omega-3 fatty acid per gram of food product. Various embodiments provide food products nutritionally enhanced with the composition to prepare food product comprising omega-3 fatty acid at a concentration of between 0.25 mg/g and 5 mg/g, between 0.25 mg/g and 4 mg/g, between 0.25 mg/g and 3.5 mg/g, between 0.25 mg/g and 3 mg/g, between 0.25 mg/g and 2.5 mg/g, between 0.25 mg/g and 2 mg/g, between 0.25 mg/g and 1.75 mg/g, between 0.25 mg/g and 1.5 mg/g, between 0.25 mg/g and 1.25 mg/g, and between 0.25 mg/g and 1 mg/g food product. In some embodiments (e.g., some fruit or vegetable preparations described herein), higher concentrations of omega-3 fatty acid are employed. In various embodiments, the omega-3 fatty acid concentration per gram of food product is between 0.5 mg/g and 10 mg/g, between 1.0 mg/g and 8 mg/g, between 1.5 mg/g and 6 mg/g, between 2 mg/g and 4 mg/g, or between 2 mg/g and 3 mg/g. The amount of omega-3 fatty acid in the composition is thus sufficient to provide the desired concentration of omega-3 fatty acid to the food product. As those of skill in art readily appreciate, this amount will vary depending on the relative amounts of the omega-3 fatty acid-containing composition and the food product that are combined.

[0208] Any suitable source of omega-3 fatty acids can be used in the compositions, food products, and methods described herein. Omega-3 fatty acids can be obtained from a vegetarian or non-vegetarian source. The omega-3 fatty acid of the composition that is added to prepare the food product can be selected from the group consisting of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and alpha-linolenic acid (ALA). If a combination of different omega-3 fatty acids is used, the total amount of DHA, EPA and/or EPA is at least 0.25 mg/g food product, and the concentration ranges recited above refer to the amount of omega-3 fatty acid, regardless of type(s). The composition can include DHA alone (i.e., as the only omega-3 fatty acid), EPA alone, or ALA alone. Alternatively, any combination of DHA, EPA and ALA can be used to nutritionally enhance the food product. For example, the composition can include DHA and ALA. The composition can include EPA and ALA. The composition can include DHA and EPA. The composition can include DHA, EPA and ALA.

[0209] The omega-3 fatty acids used in compositions and products described herein (e.g., the fruit or vegetable preparations discussed below) can be encapsulated or non-encapsulated. Encapsulated fatty acids are sometimes referred to as microencapsulated. Omega-3 fatty acids can be encapsulated by proteins, such as dairy proteins or plant proteins (see below for description of these), or modified or unmodified carbohydrates. Modifications to carbohydrates (e.g., polyaccharides) can include any modification known to those of skill in art that would be appropriate in this context. Examples include heat-modification, enzymatic modification, grinding, and co-mixing, which are the most common types of polysaccharide modification. In illustrative embodiments, omega-3 fatty acids can be encapsulated with modified polysaccharide, unmodified polysaccharide, or gelatin. Polysaccharides useful in encapculating omega-3 fatty acids include agar, carageenen, maltodextrin, corn syrup solids, pectin, xanthan gum, gellan gum, monosaccharides and disaccharides. Omega-3 fatty acids can also be encapsulated with monosaccharides or disaccharides. Other methods of micro-encapsulating omega-3 fatty acids include the formation of liposomes. Encapsulation methods for omega-3 fatty acids are reviewed in Feixollahi et al. (January 2018) Current Nutrition & Food Science 14(2):90-103, which is hereby incorporated by reference in its entirety for this description.

Protein

[0210] In various embodiments, the food product when nutritionally enhanced with the composition comprises protein at a concentration of 25-200 mg/g, 25-180 mg/g, 25-170 mg/g, 25-150 mg/g, 25-125 mg/g, 25-120 mg/g, 25-110 mg/g, 25-100 mg/g, 25-90 mg/g, 25-80 mg/g, 25-70 mg/g, 25-60 mg/g, or 25-50 mg/g. The protein can be already present in the food product prior to addition of the composition, present in the composition itself, or some combination thereof.

[0211] In various embodiments, the food product includes protein at a concentration of 25-200 mg/g, 25-180 mg/g, 25-170 mg/g, 25-150 mg/g, 25-125 mg/g, 25-120 mg/g, 25-110 mg/g, 25-100 mg/g, 25-90 mg/g, 25-80 mg/g, 25-70 mg/g, 25-60 mg/g, or 25-50 mg/g.

[0212] In certain embodiments, the food product is nutritionally enhanced with an isolated protein, which can be included in the composition or added to the food product separately from the composition. Accordingly, the composition can include sufficient protein to deliver at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 mg/g to the food product or a level of protein falling within any of the protein ranges indicated above.

[0213] The isolated protein can be any isolated protein. Isolated proteins that can be added to the food product include dairy proteins. Preferred dairy proteins are milk protein, casein, or whey. Proteins isolated from plant sources can also be used, including soy protein, pea protein, cottonseed protein, corn protein, or wheat protein. Additional protein sources that can be employed are discussed below in the subsection under Further Ingredients entitled Protein.

Illustrative Concentrations of Choline, Omega-3 Fatty Acids, and Optionally Isolated Protein

[0214] In illustrative embodiments, a composition for nutritionally enhancing a food product (e.g., stirred yogurt) can include ALA at a concentration sufficient to yield a food product having ALA at a concentration of 0.45 mg/g food product to about 4.5 mg/g food product and DHA at a concentration sufficient to yield a food product having about 0.40 mg/g food product to about 3.5 mg/g food product, along with choline (e.g., choline chloride) at a concentration sufficient to yield a food product having about 0.70 mg/g food product to about 2.5 mg/g food product. In a specific, illustrative embodiment, a composition for nutritionally enhancing a food product (e.g., stirred or drinkable yogurt) can include ALA at a concentration sufficient to yield a food product having ALA at a concentration of about 1.4 mg/g food product and DHA at a concentration sufficient to yield a food product having about 1.15 mg/g food product, along with choline (e.g., choline chloride) at a concentration sufficient to yield a food product having about 1.06 mg/g food product. If the food product is to include isolated protein, the isolated protein can, but need not, be included in the composition for nutritionally enhancing the food product. If isolated protein is included in the composition, in illustrative embodiments, the isolated protein (e.g., milk protein concentrate at 85% protein by dry matter) can be included at a concentration sufficient to yield a food product having about 15 mg/g food product to about 55 mg/g of food product.

[0215] In illustrative embodiments, the nutritionally enhanced food products (e.g., stirred yogurt) include ALA at a concentration of 0.45 mg/g food product to about 4.5 mg/g and DHA at a concentration of about 0.40 mg/g food product to about 3.5 mg/g food product in combination with choline (e.g., choline chloride) at a concentration of about 0.70 mg/g food product to about 2.5 mg/g food product. In a specific, illustrative embodiment, a nutritionally enhanced food product (e.g., stirred or drinkable yogurt) can include ALA at a concentration of about 1.4 mg/g food product and DHA at a concentration of about 1.15 mg/g food product, along with choline (e.g., choline chloride) at a concentration of about 1.06 mg/g food product. In illustrative embodiments in which isolated protein is added to the food products, the concentration of isolated protein (e.g., milk protein concentrate at 85% protein by dry matter) can be from about 15 mg/g food product to about 55 mg/g of food product. Generally, a lower amount of protein can be used if the food product includes a basic ingredient that has some protein. For example, milk typically has on the order of 35 mg protein/g, and therefore, milk-based food products may (depending on the volumes of other components added to the milk) may not need to have isolated protein added to it to produce a nutritionally enhanced food product containing at least 25 mg protein/g food product. (See, e.g., Example 1, disclosing these concentration ranges for ALA, DHA, choline, and protein.)

Methods for Preparing Compositions for Nutritionally Enhancing Food Products

[0216] A method of preparing a composition for nutritionally enhancing a food product entails combining choline or a choline salt, omega-3-fatty acid and an ingredient comprising protein or an isolated protein. In one embodiment, when a food product is nutritionally enhanced with said composition, the food product has an acceptable taste or acceptable odor as determined by a sensory panel, or the food product has a peroxide value of less than 50 mEq. In various embodiments, the peroxide value of the food product nutritionally enhanced with the composition is less than 50 mEq, less than 40 mEq, less than 35 mEq, less than 30 mEq, less than 25 mEq, less than 20 mEq, less than 15 mEq, less than 10 mEq, or less than 5 mEq.

[0217] In an embodiment of the method for preparing the composition, the choline or choline salt is first combined with the omega-3 fatty acid to prepare a choline salt/omega-3 fatty acid formulation, and the choline salt/omega-3 fatty acid formulation is combined with the ingredient comprising protein or an isolated protein.

[0218] In another embodiment, choline or choline salt, the omega-3 fatty acid and the ingredient comprising protein or an isolated protein, are combined simultaneously.

Methods for Preparing Nutritionally Enhanced Food Products

[0219] Nutritionally enhanced food products (or food compositions), such as those described above, can be prepared by combining choline and omega-3-fatty acid with at least one edible ingredient to produce a nutritionally enhanced food product (or food composition) that includes at least 0.25 mg/g choline (e.g., free-base choline, choline salt, or phosphotidylcholine), at least 0.25 mg/g omega-3 fatty acid, and optionally at least 25 mg/g protein. The resultant nutritionally enhanced food product (or food composition) has an acceptable taste or acceptable odor as determined by a sensory panel, or wherein the nutritionally enhanced food composition has a peroxide value of less than 50 mEq.

[0220] In one embodiments, the choline can be combined with the at least one edible food ingredient before the omega-3-fatty acid is combined with the at least one edible food ingredient. Alternatively, the omega-3-fatty acid can be combined with the at least one edible food ingredient before the choline is combined with the at least one edible food ingredient. In another embodiment, the choline and the omega-3 fatty acid can be combined simultaneously with the at least one edible food ingredient. This would be the case, for example, if the choline and omega-3 fatty acids had been pre-combined to form a composition for nutritionally enhancing food products (or food compostions), such as those described above. In isolated protein is added to the food product (or food compostions), it can be present in a composition for nutritionally enhancing food products or added separately. If the isolated protein is added separately, it can be added at any point for many applications, and for applications wherein the protein is best added at a particular point in preparing the nutritionally enhanced food product (or food compostions), this point will be apparent to, or readily determined by, those of skill in the art.

[0221] The choline, omega-3 fatty acids, proteins and concentrations thereof that are discussed above apply equally to the methods for preparing a nutritionally enhanced food product (e.g., food composition). The nutritionally enhanced food product (e.g., food composition) can be any food product, e.g., any of those described herein, and those of skill in the art can readily select one or more suitable edible ingredients for a given food product (e.g., food composition).

[0222] The method can additionally include any step(s) used in connection with the preparation of a particular food product (or food composition), such as mixing, blending, chopping, whipping, kneading, folding, heating, cooling, or freezing. Likewise, the method can additionally include packaging the food product (or food composition) in any suitable packaging. The packaging can be a photo-protective packaging material or a non-photo protective packaging material, optionally wherein the non-photo protective packaging material comprises polyethylene terephthalate (PET), polystyrene, polypropylene, polylactic acid (PLA), or other plant-based equivalents thereof.

Nutritionally Enhanced Food Products and their Stability

[0223] As noted above, in various embodiments, the food product when nutritionally enhanced with the composition includes choline or choline salt at a concentration of between 0.25 mg/g and 5 mg/g food product, DHA at a concentration of between 0.25 mg/g and 5 mg/g food product, and/or ALA at a concentration of between 0.25 mg/g and 5 mg/g food product. In some embodiments, the food product includes protein at a concentration of between 25 mg/g and 200 mg/g of food product, in addition to some form of choline and at least one omega-3 fatty acid. (See above for additional concentration ranges of these components.)

[0224] The food product when nutritionally enhanced with a composition provided herein is stable when exposed to light at a temperature of between 0 C. and 25 C. for at least 2 weeks, the food product has an acceptable taste or acceptable odor, or the nutritionally enhanced food product has a peroxide value of less than 50 mEq. As noted above, omega-3 fatty acids are chemically unstable and easily oxidize when exposed to UV or visible light. In various embodiments, the food product when nutritionally enhanced with the composition has an acceptable taste or acceptable odor when the nutritionally enhanced food product is exposed to light for at least 4 weeks, at least 6 weeks, at least 8 weeks, at least 10 weeks, at least 12 weeks, at least 18 weeks, at least 24 weeks, or at least 52 weeks.

[0225] The food product when nutritionally enhanced with a composition provided herein has acceptable taste, acceptable odor or a peroxide value of less than 50 mEq when the food product is stored in a non-refrigerated container or a refrigerated container. The food product can be stored in a non-refrigerated container or a refrigerated container for at least 4 weeks, at least 6 weeks, at least 8 weeks, at least 10 weeks, at least 12 weeks, at least 18 weeks, at least 24 weeks, or at least 52 weeks and have acceptable taste, acceptable odor or a peroxide value of less than 50 mEq. The refrigerated or non-refrigerated container can be a lighted or unlighted retail display case.

[0226] In certain embodiments, the food product nutritionally enhanced with a composition described herein has no, or an acceptably low level of, fishy taste, metallic taste, or rancid taste. In some embodiments, the food product nutritionally enhanced with a composition described herein has a reduced fishy taste, reduced metallic taste or reduced rancid taste as compared to a food product that is not nutritionally enhanced with choline or choline salt and omega-3 fatty acid.

[0227] In another aspect, the food product nutritionally enhanced with a composition described herein the food product nutritionally enhanced with a composition described herein has no, or an acceptably low level of, vegetable odor, fruity odor, floral odor, medicinal odor, chemical odor, fishy odor, offensive odor, painty odor or earthy odor. In some embodiments, the food product nutritionally enhanced with a composition described herein has has a reduced vegetable odor, fruity odor, floral odor, medicinal odor, chemical odor, fishy odor, offensive odor, painty odor or earthy odor as compared to a food that is not nutritionally enhanced with the composition.

[0228] The food product nutritionally enhanced with the composition is any food product that can be consumed by a human or an animal. In one embodiment, the food product is a dairy product. The dairy product can be whole milk, reduced fat milk, ultra-filtered milk, flavored milk, a flavored milk beverage, a meal replacement beverage, a protein shake, a yogurt-based smoothie, yogurt, cheese, a cheese product, liquid creamer, powdered creamer, a probiotic shot, pudding, a dessert, dip, dressing, or sauce or condiment. In some embodiments, the food product is a non-dairy liquid or powdered creamer.

[0229] In one embodiment, the food product nutritionally enhanced with the composition is a beverage. The beverage can be milk, a cultured-yogurt drink, juice, a fruit drink, a carbonated drink, a milk substitute, including soy milk, almond milk and other milk derived from plant matter, an alcoholic beverage, a coffee drink, or a tea drink.

[0230] In some embodiments, the food product nutritionally enhanced with the composition is a nut butter or seed butter. Illustrative nut and seed butters include peanut butter, almond butter, cashew butter, chestnut butter, hazelnut butter, macademia nut butter, pecan butter, walnut butter, brazil nut butter, pistachio butter, sesame seed butter (tahini), sunflower seed butter, pumpkin seed butter, and the like.

[0231] In certain embodiments, the food product nutritionally enhanced with the composition is a bar, such as a candy, granola, energy, protein, or meal replacement bar. Such bars can include complex carbohydrates, such as oats or barley, as well as sugars like fructose, glucose, maltodextrin, etc. Fats commonly included in such bars included cocoa butter and chocolate. Other ingredients can include, for example, any of the nut or seed butters noted above, and any suitable ingredient listed below in the section entitled Further Ingredients, e.g., grain, seeds, protein and sweetners. Bars can be any shape and can be enrobed in a coating, such as yogurt or chocolate.

[0232] The food product nutritionally enhanced with the composition provided herein are fresh, frozen, or shelf-stable. In an embodiment, the food product is canned or retorted.

[0233] Nutritionally enhance food products can include additional ingredients, such as one or more of those described in the section below entitled Further Ingredients.

[0234] Food products nutritionally enhanced with the composition that are frozen foods include ice cream, soft serve ice cream, ice cream novelties, frozen desert, frozen yogurt, popsicles, frozen entrees, frozen prepared foods, frozen dinners, frozen lunches, and frozen breakfasts.

Illustrative Formulations

[0235] Illustrative formulations for stirred yogurt, suitable for cups and tubes, are given in the Tables below.

TABLE-US-00001 Ingredient % by weight Whole Milk, Fluid, 3.5% Milk Fat, non-GMO 80-95 Milk protein concentrate at 85% protein by dry matter 2-6 Skim milk powder-low temperature dry 0.5-3% Starch, Tapioca, 1-4 Flax Oil, 50% ALA 0.1-0.8 Pectin/Agar 0.1-0.8 DHA, 40% 0.1-0.8 Choline Chloride, 74% 0.1-0.3 Locust Bean Gum POR/A Powder 0.1-0.3 Starter Culture (Steptococcus thermophilus, Lactobacillus 0.01-0.4% delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactose) Mixed Tocopherol 95 0.001-0.005

TABLE-US-00002 Ingredient % of Formulation Whole Milk 70.24-89.9% Stabilizer Blend 1.2-3.03% Non Fat Dry Milk 1.4-4.8% Flax Oil (50% ALA) .11-.95% Algal Oil (40% DHA) .11-.95% Choline Chloride (74% Choline) .05-.495% Culture .001-.04% Antioxidant .001-.04% Fruit Prep 9.95-19.95%

[0236] Illustrative formulations for yogurt drinks are given in the Tables below.

TABLE-US-00003 Ingredient (% by weight) Whole Milk, Fluid, 3.5% Milk Fat 60-75 Starch, Tapioca, Native 0.2-0.8 MPC-85 1-4 Gellan Gum 0.02-0.12 Water 25-40 DHA, 40% 0.1-0.25 Flax Oil, 50% ALA 0.1-0.25 Choline Chloride, 74% 0.05-0.15 Mixed Tocopherol 95 0.001-0.01 Starter Culture (Streptococcus 0.01-0.4% thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactose) Total 100%

TABLE-US-00004 Ingredient % of Formulation Whole Milk 40-60.5% Tapioca Starch .1-2.05% Milk Protein Concentrate 1.2-3% Gellan Gum .01-1.05% Water 15.5-30% Flax Oil (50% ALA) .11-.95% Algal Oil (40% DHA) .11-.95% Choline Chloride (74% Choline) .05-.495% Culture .001-.04% Antioxidant .001-.04% Fruit Prep 9.95-19.95%

[0237] An illustrative formulation for a chocolate milk/milk drink is given in the Table below.

TABLE-US-00005 Flavored milk % by Weight Ingredients % Milk 1.2% BF, 10.2% total solids 85-95 Cocoa powder-defatted or full fat, alkalized or not 1-6 Sugar-fructose, sucrose, or other 3-10 Stabilizer-carrageenan, locust bean gum 0.1-2.0 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax oil ALA .05-2.0 Mixed tocopherols (0.07%) 0.05-0.1 Rosemary extract 0.01-0.1 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

[0238] An illustrative formulation for string cheese/sticks is given in the Table below.

TABLE-US-00006 Ingredients % by Weight Milk 1-5% BF 85-95 Cheese culture .001-.04% Rennet .001-.04% MPC or WPC protein fortification 0.2-1.5 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax oil ALA .05-2.0 Mixed tocopherols (0.07%) 0.05-0.1 Salt 0.1-4.0 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

[0239] An illustrative formulation for a chocolate flavored pudding is given in the Table below.

TABLE-US-00007 Ingredients % by Weight Milk 1-5%% BF 85-95 Cocoa powder or natural flavors 0.5-5 Starch-Non-gmo tapioca or other 0.5-2.5 Stabilizer-carrageenan, 0.1-2.0 Locust bean gum or other Omega-3 fatty acid powder .05-4 Choline 0.01-0.2 flax oil ALA .05-2.0 Mixed tocopherols (0.07%) 0.05-0.1 Salt 0.1-4.0 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

[0240] An illustrative formulation for a non-dairy creamer is given in the Table below.

TABLE-US-00008 MTC Powder 32-45% Choline Bitartrate 2.8-4.25% Cane Sugar 25-42% Omega-3 fatty acid Powder 12-18.85% Vanilla 7.75-12.25% Disodium Phosphate Dihydrate 2.65-2.95% *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

Fruit or Vegetable Preparations Including Choline and Omega-3 Fatty Acid

[0241] In some embodiments, a fruit or vegetable preparation is nutritionally enhanced with choline and omega-3 fatty acid.

[0242] The oxidation of polyunsaturated fatty acids, such as omega-3 fatty acids, produce fruit or vegetable preparations supplemented with omega-3 fatty acids with unacceptable appearance, flavor, taste, aroma, odor or texture. It has been surprisingly and unexpectedly discovered that fruit or vegetable preparations having high levels of choline or choline salt, and high levels of omega-3 fatty acids with acceptable appearance, aroma, flavor or texture can be prepared. In addition, the fruit or vegetable preparations including high levels of choline or choline salt and high levels of omega-3 fatty acids are shelf stable and have acceptable appearance, aroma, flavor and/or texture after exposure to a temperature of between 10 C. to 50 C., 20 C. to 50 C. or 30 C. to 50 C. for a period of between 9 to 52 weeks.

[0243] In various embodiments, the fruit or vegetable preparations have peroxide values of less than 50 mEq. In one aspect, the peroxide value of the fruit or vegetable preparation is less than 50 mEq, less than 40 mEq, less than 35 mEq, less than 30 mEq, less than 25 mEq, less than 20 mEq, less than 15 mEq, less than 10 mEq, or less than 5 mEq. In another aspect, the peroxide value of the fruit or vegetable preparation is between 1-50 mEq, 1-45 mEq, 1-40 mEq, 1-35 mEq, 1-30 mEq, 1-25 mEq, 1-24 mEq, 1-23 mEq, 1-22 mEq, 1-21 mEq, 1-20 mEq, 1-17.5 mEq, 1-15 mEq, 1-12.5 mEq, and 1-10 mEq. Peroxide values are measured in commercially available machines and can be performed according to AOCS Official Method Cd 8-53, ISO/IEC 17025, or other known methods.

[0244] In certain embodiments, the fruit or vegetable preparation includes 100 mg to 1000 mg of omega-3 fatty acid per 100 grams of the fruit or vegetable preparation (mg/100 g) and includes 50 mg to 500 mg of choline per 100 grams of fruit or vegetable purees. Unless otherwise indicated, the unit mg/g refers to milligrams of constituent (e.g., choline or omega-3 fatty acid) per gram of fruit or vegetable preparation. Accordingly, the phrase at least 0.25 mg/g choline salt means at least 0.25 mg choline salt per gram of food product, and the phrase at least 100 mg/100 g omega-3 fatty acid means at least 100 mg omega-3 fatty acid per 100 g of fruit or vegetable preparation.

[0245] In various embodiments, the total amount of DHA, EPA and/or EPA is 100 mg/100 g to 1000 mg/100 g omega-3 fatty acid, between 100 mg/100 g to 750 mg/100 g omega-3 fatty acid, between 100 mg/100 g to 500 mg/100 g omega-3 fatty acid, between 100 mg/100 g to 400 mg/100 g omega-3 fatty acid, between 150 mg/100 g to 400 mg/100 g, between 150 mg/100 g to 350 mg/100 g, between 200 mg/100 g to 350 mg/100 g, or between 200 mg/100 g to 300 mg/100 g omega-3 fatty acid.

[0246] In various embodiments, the fruit or vegetable preparation provides choline or choline salt at a concentration of between 50 mg/100 g to 500 mg/100 g choline, between 50 mg/100 g to 400 mg/100 g choline, between 50 mg/100 g to 350 mg/100 g choline, between 50 mg/100 g to 300 mg/100 g choline, between 50 mg/100 g to 250 mg/100 g choline, between 50 mg/100 g to 200 mg/100 g choline, between 75 mg/100 g to 250 mg/100 g choline, between 75 mg/100 g to 200 mg/100 g choline, or between 100 mg/100 g to 200 mg/100 g choline.

[0247] Any of the above described cholines and omega-3 fatty acids (including encapsulated omega-3 fatty acids) and combinations thereof can be used in the fruit or vegetable preparations described herein.

[0248] The fruit or vegetable preparations can include additional ingredients, such as one or more of those described in the section below entitled Further Ingredients.

[0249] Illustrative fruit or vegetable preparations include apple sauce, pear sauce, banana sauce, strawberry sauce, or combinations of two or more fruits and vegetables, such as, e.g., strawberry apple sauce and apple banana sauce.

[0250] In illustrative embodiments, the fruit or vegetable preparations (e.g., apple sauce, cinnamon apple sauce, or strawberry apple sauce) can include omega-3 fatty acids (e.g., from omega-3 fatty acid powder) at a concentration of about 5 mg/g fruit or vegetable preparation to about 15 mg/g fruit or vegetable preparation (e.g., 10 mg/g fruit or vegetable preparation) in combination with choline (e.g., choline chloride) at a concentration of about 0.70 mg/g fruit or vegetable preparation to about 3.5 mg/g fruit or vegetable preparation (e.g., 1.8 mg/g fruit or vegetable preparation). (See, e.g., Example 13.) In illustrative embodiments in which isolated protein is added to the fruit or vegetable preparation, the concentration of isolated protein (e.g., milk protein concentrate at 85% protein by dry matter) can be from about 15 mg/g food fruit or vegetable preparation to about 55 mg/g of fruit or vegetable preparation. (The illustrative apple sauce, cinnamon apple sauce, and strawberry apple sauce shown in Example 13 do not include any added isolated protein.

[0251] In illustrative embodiments, a composition for nutritionally enhancing a fruit or vegetable preparation (e.g., apple sauce, cinnamon apple sauce, or strawberry apple sauce) can include omega-3 fatty acid at a concentration sufficient to yield a fruit or vegetable preparation having omega-3 fatty acids (e.g., from omega-3 fatty acid powder) at a concentration of about 5 mg/g fruit or vegetable preparation to about 15 mg/g fruit or vegetable preparation (e.g., 10 mg/g fruit or vegetable preparation) along with choline (e.g., choline chloride) at a concentration sufficient to yield a a fruit or vegetable preparation having about 0.70 mg/g food product to about 3.5 mg/g food product. If the fruit or vegetable preparation is to include isolated protein, the isolated protein can, but need not, be included in the composition for nutritionally enhancing the fruit or vegetable preparation. If isolated protein is included in the composition, in illustrative embodiments, the isolated protein (e.g., milk protein concentrate at 85% protein by dry matter) can be included at a concentration sufficient to yield a food product having about 15 mg/g fruit or vegetable preparation to about 55 mg/g of fruit or vegetable preparation.

Illustrative Formulations

[0252] Illustrative formulations for applesauce are given in the Tables below.

TABLE-US-00009 Applesauce % by Weight Ingredients % Apple puree 80-90 Apple juice concentrate 5-10 Apple puree concentrate 2-8 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax powder ALA .05-2.0 Encapsulated ALA Mixed tocopherols (0.07%) 0.05-0.1 Rosemary extract 0.01-0.1 Ascorbic acid 0.01-0.1

TABLE-US-00010 % of Formulation by Ingredients weight Apple Base (e.g., 87.5-95% Apple 87.5-99 puree and 5.5-10% Apple puree concentrate) Omega-3 fatty acid powder 0.5-1.95* Choline Chloride (74% Choline) 0.11-0.25 Mixed tocopherols (0.07%) (Antioxidant) 0.01-0.05 Rosemary extract 0.01-0.05 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

[0253] In some embodiments, the formulation given in the Table above can include 0.01-0.05% ascorbic acid.

Methods for Preparing Fruit or Vegetable Preparations

[0254] This disclosure also provides a method for preparing a fruit or vegetable preparation. The method of preparing the fruit or vegetable preparation includes: [0255] a. providing a base fruit or vegetable puree; [0256] b. combining choline and omega-3-fatty acid with the fruit or vegetable puree to prepare puree A; [0257] c. subjecting puree A to a temperature that pasteurizes puree A; and [0258] d. cooling the fruit or vegetable preparation to ambient temperature, wherein the fruit of vegetable preparation has an acceptable appearance, aroma, flavor and texture as determined by a sensory panel, or wherein the fruit or vegetable puree has a peroxide value of less than 50 mEq.

[0259] In some embodiments, the pasteurization is performed at a temperature of from 65 C. to 100 C., from 65 C. to 95 C., from 65 C. to 90 C., from 65 C. to 85 C., from 65 C. to 80 C., from 65 C. to 75 C., from 65 C. to 70 C., from 70 C. to 100 C., from 70 C. to 95 C., from 70 C. to 90 C., from 70 C. to 85 C., from 70 C. to 85 C., from 70 C. to 80 C., from 70 C. to 75 C., from 75 C. to 100 C., from 80 C. to 95 C., or from 85 C. to 90 C. for a period of from 1 second to 180 seconds, from 1 second to 120 seconds, from 1 second to 90 seconds, from 1 second to 60 seconds, from 1 second to 30 seconds, from 5 seconds to 120 seconds, from 5 second to 90 seconds, from 5 seconds to 60 seconds, from 5 seconds to 30 seconds, from 10 seconds to 120 seconds, from 10 seconds to 90 seconds, from 10 seconds to 60 seconds, from 10 seconds to 30 seconds or from 10 seconds to 20 seconds. In some embodiments, pasteurization is performed for longer time periods, such as 3, 5, 5, 6, 7, 8, 9, 10, 11, 12, 13 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 minutes. In illustrative embodiments, pasterization is performed at a temperature of from 65 C. to 100 C. for a period of from 1 second to 30 minutes; a temperature of from 80 C. to 96 C. for a period of from 3 to 25 minutes; a temperature of from 82 C. to 94 C. for a period of from 3 to 20 minutes; a temperature of from 84 C. to 92 C. for a period of from 3 to 15 minutes; or a temperature of from 85 C. to 90 C. (e.g., 88 C.) for a period of from 3 to 10 minutes (e.g., about 5 minutes).

[0260] In various embodiments, methods for preparing a fruit or vegetable preparation can include adding one or more additional ingredients to the base fruit or vegetable puree, puree A, or the fruit or vegetable preparation itself.

[0261] The methods for preparing a fruit or vegetable preparation disclosed herein can be used to prepare apple sauce, pear sauce, banana sauce, strawberry sauce, or combinations of two or more fruits and vegetables. Exemplary sauces include apple sauce, strawberry apple sauce, apple banana sauce.

[0262] In illustrative embodiments, the fruit or vegetable preparations (e.g., apple sauce, cinnamon apple sauce, or strawberry apple sauce) can include omega-3 fatty acids (e.g., from omega-3 fatty acid powder) at a concentration of about 5 mg/g fruit or vegetable preparation to about 15 mg/g fruit or vegetable preparation (e.g., 10 mg/g fruit or vegetable preparation) in combination with choline (e.g., choline chloride) at a concentration of about 0.70 mg/g fruit or vegetable preparation to about 3.5 mg/g fruit or vegetable preparation (e.g., 1.8 mg/g fruit or vegetable preparation). (See, e.g., Example 13.) Such preparations can be pasteurized at a temperature of between 85 C. to 90 C. (e.g., about 88 C. [190 F.]).

Stability of Fruit or Vegetable Preparations

[0263] The methods of preparing the fruit or vegetable preparation produce fruit or vegetable preparations that have acceptable appearance, aroma, flavor and texture after exposure to a temperature of between 10 C. to 50 C. for a period of between 9 to 52 weeks, between 10 C. to 50 C. for a period of between 9 to 48 weeks, between 10 C. to 50 C. for a period of between 9 to 26 weeks, between 10 C. to 50 C. for a period of between 9 to 18 weeks, between 10 C. to 50 C. for a period of between 9 to 12 weeks, or after exposure to a temperature of between 20 C. to 50 C. for a period of between 9 to 52 weeks, between 20 C. to 50 C. for a period of between 9 to 48 weeks, between 20 C. to 50 C. for a period of between 9 to 26 weeks, between 20 C. to 50 C. for a period of between 9 to 18 weeks, or between 20 C. to 50 C. for a period of between 9 to 12 weeks, or after exposure to a temperature of between 30 C. to 50 C. for a period of between 9 to 52 weeks, between 30 C. to 50 C. for a period of between 9 to 48 weeks, between 30 C. to 50 C. for a period of between 9 to 26 weeks, between 30 C. to 50 C. for a period of between 9 to 18 weeks, or between 30 C. to 50 C. for a period of between 9 to 12 weeks. These temperature ranges and times can be used for accelerated stability testing of the fruit or vegetable preparation acceptable appearance, aroma, flavor, and/or texture or for long-term storage of the fruit or vegetable preparation.

[0264] In some embodiments, a fruit or vegetable preparation that has acceptable appearance, aroma, flavor, and/or texture or a peroxide value of less than 50 mEq when the fruit or vegetable preparation is stored in a non-refrigerated container or a refrigerated container. The fruit or vegetable preparation can be stored in a non-refrigerated container or a refrigerated container for at least 4 weeks, at least 6 weeks, at least 8 weeks, at least 10 weeks, at least 12 weeks, at least 18 weeks, at least 24 weeks, or at least 52 weeks and have acceptable appearance, aroma, flavor and/or texture or a peroxide value of less than 50 mEq. The refrigerated or non-refrigerated container can be a lighted or unlighted retail display case.

[0265] In certain embodiments, the fruit or vegetable preparations disclosed herein are stable when exposed to light at a temperature of between 0 C. and 25 C. for at least 2 weeks. This is surprising because omega-3 fatty acids are chemically unstable and easily oxidize when exposed to UV or visible light. More specifically, the fruit or vegetable preparation has an acceptable taste or acceptable odor, or the fruit or vegetable preparation has a peroxide value of less than 50 mEq after this light exposure. In one aspect, the fruit or vegetable preparation has an acceptable appearance, flavor, taste, aroma, odor, and/or texture when the fruit or vegetable preparation is exposed to light for at least 4 weeks, at least 6 weeks, at least 8 weeks, at least 10 weeks, at least 12 weeks, at least 18 weeks, at least 24 weeks, or at least 52 weeks.

Further Ingredients

[0266] In various embodiments, the compositions, food products, and fruit and vegetable preparations described herein can include one or more of the following additional ingredients. Similarly, the methods described herein can include one or more steps of adding any of these ingredients or combinations thereof.

Iron

[0267] The compositions, food products, and fruit and vegetable preparations described herein can further include iron, and the methods disclosed herein can further include the step of adding iron. The iron should be in a form that is bioavailable and sufficiently stable for the particular application and should not adversely affect the taste of the composition, food product, or fruit and vegetable preparation to which it is added. In some embodiments, these aims are achieved by encapsulating (also termed miroencapsulating) iron. Encapsulation methods are well known and include the use of materials discussed above in connection with the description of encapsulated omega-3 fatty acids.

[0268] Methods of encapsulating iron, specifically, are described in PCT Pub. No. WO2010040789, which is hereby incorporated by reference in its entirety for this description. This publication describes, in particular, encapsulated iron having a core including iron alginate and an outer layer of calcium alginate. The core can additionally include a bioavailable iron salt, such as ferric saccharate. The core can additionally include sodium alginate. The core can additionally include a chelating agent, such as saccharose. An encapsulated iron that is commercially available from AB-Fortis is AB-FORTIS Microencapsulated Iron, which includes ferric sccharate (40% iron w/w) as the active agent, and calcium alginate including sodium alginate and calcium acetate.

[0269] In illustrative embodiments, an encapsulated iron, such as AB-Fortis is AB-FORTIS Microencapsulated Iron, can be included in a food product or fruit and vegetable preparation at a concentration of about 0.25 mg/100 g of food product/fruit and vegetable preparation to about 20 mg/100 gm of food product/fruit and vegetable preparation. In various embodiments, the concentration of encapsulated iron can be from about between 0.25 mg/g and 18 mg/g, between 0.25 mg/g and 16 mg/g, between 0.25 mg/g and 14 mg/g, between 0.50 mg/g and 12 mg/g, between 0.50 mg/g and 10 mg/g, between 0.50 mg/g and 8 mg/g, between 0.50 mg/g and 6 mg/g, between 0.75 mg/g and 4 mg/g, between 0.75 mg/g and 3 mg/g, between 1 mg/g and 3 mg/g, and between 2 mg/g and 3 mg/g. In illustrative embodiments, the amount of encapsulated iron in a composition for nutritionally enhancing food products is sufficient to provide the desired concentration of iron to the food product. As those of skill in art readily appreciate, this amount will vary depending on the relative amounts of the iron-containing composition and the food product that are combined.

[0270] Encapsulated iron is particularly useful for nutritionally enhancing foods, such as dairy-based products like, milk, yogurt, ice cream, or cheese that cannot readily be enhanced with non-encapsulated iron.

Antioxidants

[0271] The compostions, food products, and fruit and vegetable preparations described herein can further include an antioxidant, and the methods disclosed herein can further include the step of adding an antioxidant. The antioxidant(s) can be selected from the group consisting of beta-carotene, lycopene, zeaxanthin, lutein, tocopherols, tocotrienols, rosemary extract, vitamin A, vitamin B3, vitamin C (ascorbic acid), resveratrol, green tea extract, coffeeberry extract, grape seed extract, blueberry extract, and goji berry extract. In various embodiments, the amount of antioxidant(s) by mass (e.g., in a fruit or vegetable preparation) can be between 0.005% to 0.5%, between 0.005% to 0.4%, between 0.005% to 0.3%, between 0.005% to 0.2%, between 0.005% to 0.1%, between 0.01% to 0.1%, between 0.01% to 0.09%, between 0.01% to 0.08% between 0.01% to 0.07%, between 0.01% to 0.6%, or between 0.01% to 0.05%.

Grain and/or Seeds

[0272] The compostions, food products, and fruit and vegetable preparations described herein can further include grain and/or seeds, and the methods disclosed herein can further include the step of adding a grain and/or seeds. Any grain or seed and be employed for this purpose. Grains can be selected from the group consisting of amaranth, barley, buckwheat, millet, oat, rice, rye, sorghum, spelt, teff, triticale, wheat, quinoa, and wild rice. Seeds can be selected from the group consisting of chia, flax, hemp, mustard, poppy, pumpkin and sunflower. In various embodiments, the amount of grains or seed by mass (e.g., in a fruit or vegetable preparation) can be between 1% to 20%, between 1% to 15%, between 1% to 10%, between 2% to 10%, between 3% to 10%, between 4% to 10% or between 5% to 10%.

Protein

[0273] Embodiments in which protein is present with choline and omega-3 fatty acids are discussed above. Without being bound by any particular theory, it is thought that the presence of protein with choline and/or omega-3 fatty acids helps to stabilize one of both components. However, isolated protein can be added to any of the compostions, food products, and fruit and vegetable preparations described herein simply to increase the protein content of the compostions, food products, and fruit and vegetable preparations. Isolated proteins that can be added to the compostions, food products, and fruit and vegetable preparations described herein can, for example, be selected from plant protein, pulse protein, nut protein, algal protein, dairy protein, and mycoprotein. Illustrative isolated plant proteins include barley protein, oat protein, rice protein, wheat protein, and potato protein. Illustrative isolated pulse proteins include common bean protein (Phaseolus vulgaris), chickepea protein, fava bean protein, lentil protein, lima bean protein, mung bean protein, peanut protein, and soy protein. Illustrative isolated nut proteins include almond protein, cashew protein, chestnut protein, hazelnut protein, macademia nut protein, pecan protein, pistachio protein and walnut protein. Illustrative isolated algal proteins include Spirulina protein, Chlorella protein, blue-green algae protein, green algae protein and red algae protein. Illustrative isolated dairy proteins include whey protein and casein. Illustrative isolated mycoproteins include Saccharomyces protein and Fusarium protein. The amount of protein by mass (e.g., in a fruit or vegetable preparation) can between 0.05% to 5%, between 0.05% to 4%, between 0.05% to 3%, between 0.1% to 5%, between 0.1% to 4%, between 0.1% to 3%, between 0.2% to 5%, between 0.2% to 4%, between 0.2% to 3%, between 0.5% to 5%, between 0.5% to 4%, between 0.5% to 3%, between 0.75% to 5%, between 0.75% to 4%, between 0.75% to 3%, between 1% to 5%, between 1% to 4%, between 1% to 3% or between between 1% to 2%. In the case of protein bars and drings, the level of protein is typically higher, e.g., between 10 and 25 grams per serving.

Probiotic and/or Active Cultures

[0274] In some embodiments, the compostions, food products, and fruit and vegetable preparations described herein can further include include a probiotic or active cultures. The probiotic or active culture(s) can be selected from the group consisting of Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, or any combination thereof. Probiotic or active cultures are useful in products like yogurt or yogurt-based drinks.

Sweeteners

[0275] In some embodiments, a sweetener can be included in the compostions, food products, and fruit and vegetable preparations described herein. Illustrative sweeteners include sucrose, glucose, fructose, corn syrup, monk fruit extract, sucromalt, allulose, agave, honey, maple syrup, erythritol, maltitol, sorbitol, mannitol, xylitol, xylose, stevia/rebaudioside; neotame, brazzein, caramel, coconut sugar, maltodextrin; sorghum syrup, tagatose, aspartame, acesulfame-K, saccharine, sucralose, and cyclamates.

Packaging

[0276] The compostions, food products, and fruit and vegetable preparations described herein can be packaged in a non-photo protective packaging material. The non-photo protective packaging material can include polyethylene terephthalate (PET), polystyrene, polylactic acid (PLA), or other plant-based equivalents thereof.

EXAMPLES

Example 1Stirred Yogurt, for Cup and Tube

TABLE-US-00011 Ingredient % by weight Whole Milk, Fluid, 3.5% Milk Fat, non-GMO 80-95 Milk protein concentrate at 85% 2-6 protein by dry matter Skim milk powder-low temperature dry 0.5-3% Starch, Tapioca, 1-4 Flax Oil, 50% ALA 0.1-0.8 Pectin/Agar 0.1-0.8 DHA, 40% 0.1-0.8 Choline Chloride, 74% 0.1-0.3 Locust Bean Gum POR/A Powder 0.1-0.3 Starter Culture (Streptococcus 0.01-0.4% thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactose) Mixed Tocopherol 95 0.001-0.005

Process

[0277] 1) Batch gmo or non-gmo milk to 3.5% milk fat and 12.5% total solids [0278] 2) Heat milk to 80-10 F. [0279] 3) Batch 1Blend in warm milk the Milk Protein Concentrate in high shear liquefier for 10 minutes [0280] 4) Batch 2blend in warm milk the skim milk powder in high shear liquefier for 10 minutes [0281] 5) Batch 3blend in warm milk the tapioca starch, locust bean gum, pectin/agar blend [0282] 6) Sent to batch tank. Test for total solids and fat to confirm composition against standard [0283] 7) Pump to HTST (High temperature/short time), and add DHA, Mixed Tocopherol, and Flax oil (ALA) to the balance tank prior to pasteurization. [0284] 8) Pasteurize between 170 F-190 F [0285] 9) Homogenize through standard homogenizer (Gaulen) in two stages. First stage 1000-1500 psi, and second stage 0-500 psi [0286] 10) Pump to culture tank, and take composition against standards [0287] 11) Add culture to tank at 103-11 F., and blend for 20 mins [0288] 12) Shut off agitation and allow to culture until the white mass reaches a pH of 4.4-4.9 [0289] 13) Cool to 50 F under agitation. Once 50 F is achieved, shut off agitation

[0290] Addition of fruit preparation and flavoring system

Fruit Prep

TABLE-US-00012 Ingredient % by weight Water 40-60 Sugar, Cane, non-GMO 35-46 Natural flavor 2-7 Starch, Tapioca, Native 1-5 Flavor enhancer 1-4 Color 0.5-2.0 Locust Bean Gum POR/A Powder 0.1-0.5 Monk Fruit Extract 0.1-1.0 [0291] 14) Add fruit prep/flavor system to white mass yogurt and blend for 10 mins [0292] 15) Send to packaging

Example 2Drinkable Yogurt

TABLE-US-00013 Ingredient % by weight Whole Milk, Fluid, 3.5% Milk Fat 60-75 Starch, Tapioca, Native 0.2-0.8 MPC-85 1-4 Gellan Gum 0.02-0.12 Water 25-40 DHA, 40% 0.1-0.25 Flax Oil, 50% ALA 0.1-0.25 Choline Chloride, 74% 0.05-0.15 Mixed Tocopherol 95 0.001-0.01 Starter Culture (Streptococcus thermophilus, Lactobacillus 0.01-0.4% delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis, Lactose) Total 100%

Process

[0293] 1) Batch non-gmo milk to 3.5% BF and 12.5% total solids [0294] 2) Heat milk to 80-10 F. [0295] 3) Batch 1Blend in warm milk the Milk Protein Concentrate in high shear liquefier for 10 minutes [0296] 4) Batch 3blend in warm milk the tapioca starch, and gelan gum [0297] 5) Add water [0298] 6) Send to batch tank. Test for total solids and fat to confirm composition against standard [0299] 7) Pump to HTST (High temperature/short time), and add DHA, Mixed Tocopherol, and Flax oil (ALA) to the balance tank prior to pasteurization. [0300] 8) Pasteurize between 170 F-190 F [0301] 9) Homogenize through standard homogenizer (Gaulen) in two stages. First stage 1000-1500 psi, and second stage 0-500 psi [0302] 10) Pump to culture tank, and take composition against standards [0303] 11) Add culture to tank at 103-11 F., and blend for 20 mins [0304] 12) Shut off agitation and allow to culture until the white mass reaches a pH of 4.4-4.9 [0305] 13) Cool to 50 F under agitation. Once 50 is achieved, shut off agitation [0306] 14) Fruit was prepared according the Example 1 [0307] 15) Add fruit prep/flavor system to white mass yogurt and blend for 10 mins

Example 3Apple Sauce

TABLE-US-00014 Applesauce % by Weight Ingredients % Apple puree 80-90- Apple juice concentrate 5-10 Apple puree concentrate 2-8 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax powder ALA .05-2.0 Encapsulated ALA Mixed tocopherols (0.07%) 0.05-0.1 Rosemary extract 0.01-0.1 Ascorbic acid 0.01-0.1 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

Process

[0308] 1) Mix apple puree, concentrates and juices in vat [0309] 2) Blend for 10 mins [0310] 3) Add choline Chloride, DHA, ALA flax [0311] 4) Blend for 10 mins [0312] 5) Vat pasteurize, or tube in tube pasteurize at 170-190 F for 10 mins [0313] 6) Cool to 170 [0314] 7) Hot fill

Example 4Chocolate Milk/Milk Drink

TABLE-US-00015 Flavored milk % by Weight Ingredients % Milk 1.2% BF, 10.2% total solids 85-95 Cocoa powder-defatted or full fat, alkalized or not 1-6 Sugar-fructose, sucrose, or other 3-10 Stabilizer-carrageenan, locust bean gum 0.3-2.0 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax oil ALA .05-2.0 Mixed tocopherols (0.07%) 0.05-0.1 Rosemary extract 0.01-0.1 *The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

Process

[0315] 1) Blend milk at 0.05-2.5% [0316] 2) Add milk to blender, and add cocoa powder, sugar, stabilizer [0317] 3) Pump to HTST [0318] 4) Add functional oils (DHA, ALA, choline, tocopherols) to the balance tank [0319] 5) Pasteurize at 171-190 and homengize 2 stage 1000-1500 first stage, 0-500 second stage, or UHT at 230 C for 15 seconds [0320] 6) Cool to 40-50 F off regen or cooling section of the HTST [0321] 7) Package

Example 5String Cheese/Sticks

TABLE-US-00016 Ingredients % by Weight Milk 1-5%% BF 85-95 Cheese Culture .001-.04% Rennet .001-.04% MPC or WPC protein fortification 0.4-1.5 Omega-3 fatty acid powder .05-4* Choline chloride 0.01-0.2 flax oil ALA .05-2.0 Mixed tocapherols (0.07%) 0.05-0.1 Salt 0.1-4.0 The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product.

Process

[0322] 1) Add milk to liquefier/shear blender [0323] 2) Add DHA, ALA, Choline, Tocopherols, and rosemary extract to milk [0324] 3) Add WPC/MPC as required [0325] 4) Pasteurize at 171-190 and homogenize 2 stage 1000-1500 first stage, 0-500 second stage [0326] 5) Add milk blend to cheese vat at 80-11 F. [0327] 6) Add culture and rennet [0328] 7) Culture to 4.6-4.8 pH [0329] 8) Cut curd with 0.5-1 inch cutting screens [0330] 9) Allow curd to set and cook to 100-150 F [0331] 10) Drain whey and wash curd. Add salt [0332] 11) Add to cooker stretcher machine and heat to 120-160 F [0333] 12) Extrude cheese in twin screw extruder [0334] 13) Pack sticks/string cheese and cool to 40 F

Example 6Flavored Pudding

TABLE-US-00017 Ingredients % by Weight Milk 1-5%% BF 85-95 Cocoa powder or natural flavors 0.5-5 Starch-Non-gmo tapioca or other 0.5-2.5 Stabilizer-carrageenan, Locust bean gum or other 0.1-2.0 Omega-3 fatty acid powder .05-4 Choline 0.01-0.2 flax oil ALA .05-2.0 Mixed tocopherols (0.07%) 0.05-0.1 Salt 0.1-4.0

Process

[0335] 1) Add milk to liquefier or shear blender [0336] 2) Add cocoa powder, or natural flavor blends [0337] 3) Add starch [0338] 4) Add stabilizer [0339] 5) Add DHA, ALA, Choline [0340] 6) Vat pasteurize or tube in tube pasteurize at 170-190 f [0341] 7) Homogenize at 1000-1500 psi first stage, and 0-500 psi second stage or use colloid mill [0342] 8) Cool to 40-50 F or to 150-170 F [0343] 9) Package

Example 7Peroxide Value Determination

[0344] The peroxide value is tested according to AOAC method 965.33

Example 8Sensory Panel Testing

[0345] A sensory panel is convened to determine if the nutritionally enhanced food product has an acceptable taste or acceptable odor. Trained or untrained panelists are convened and are asked to evaluate the nutritionally enhanced food product for randity/oxidization of fats when the nutritionally enhanced food product is prepared. Next, the same panel or a different pane is asked to evaluate the nutritionally enhanced food product after the nutritionally enhanced food product was stored for a specified amount of time and temperature. The panelists are asked to rate the oxidation on a 9-point hedonic scale for rancidity (1-rancid; 9-not rancid), painty (1-painty; 9-not painty) and overall preference (1-dislike extremely; 9-like extremely). A nutritionally enhanced food product has acceptable taste or acceptable odor if the score is between 4-9.

Example 9Sensory Panel Testing of Yogurt Drink

[0346] Yogurt was prepared according to Example 2 and evaluated by a sensory panel on day 0, 14, 28, 42 and 56 days. On day 0, single-serve bottles of strawberry yogurt drinks were assigned to dark control, lighted dairy case (700 Lx), or 2000 Lx LED light box at 4 C. per the timepoint structure below.

TABLE-US-00018 Lighted Dairy Case Dark control control (700 Lx) 2000 lx light box Times: Dark Time 0, 14 days, 28 days, 42 Time 0, 7 days, control will be days*, 56 days 14 days, 21 days* evaluated each time dairy case or light box yogurts are evaluated *Fishy flavor detected by all panelists at this timepoint

Descriptive Sensory Analysis

[0347] Overhead lights were off during all preparations of yogurts. Yogurts were dispensed into clear 2-oz lidded souffle cups with 3-digit codes for descriptive sensory analysis and were served with spring water and unsalted crackers for palate cleansing. Descriptive analysis used a 0- to 15-point universal intensity scale with the SPECTRUM method and a previously established sensory language. Paper ballots were used. A descriptive sensory panel (n=7, 5 females, 2 males, ages 22-46 y) with more than 200 h experience with the descriptive analysis of dairy flavors and textures evaluated the yogurts. Consistent with SPECTRUM descriptive analysis training, panelists were presented with reference solutions of sweet, sour, salty, and bitter tastes to learn to consistently use the universal intensity scale (Meilgaard and others 1999; Drake and Civille 2003). Following consistent use of the Spectrum scale with basic tastes, panelists learned to identify and scale flavor descriptors using the same intensity scale through presentation and discussion of flavor definitions and references. Analysis of data collected from training sessions confirmed that panel results were consistent and that terms were not redundant, consistent with previous use of the developed language (Drake and others 2001; Drake and others 2005). Each panelist evaluated each yogurt in duplicate.

Statistical Analysis

[0348] Data were analyzed using analysis of variance (ANOVA) (XLSTAT, version 2014 Addinsoft Inc., New York, NY). All statistical analysis was carried out at a 95% significance level.

TABLE-US-00019 TABLE 1a Sensory profile of yogurt at time 0 Attribute Dark control Aroma intensity 4.2 In-mouth Cooked/milky 3.6 Sweet aromatic/vanillin 1.6 Fruity 3.2 Sour aromatic 1.5 Cardboard/mushroom ND* Fishy ND* Sweet taste 8.4 Sour taste 1.5 Metallic 2.2 Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. *NDnot detected

TABLE-US-00020 TABLE 1b Sensory profile of yogurt at day 7 Attribute Dark control Light box Aroma intensity 3.8a 3.3b In-mouth Cooked/milky 3.6a 3.2b Sweet aromatic/vanillin 1.5a 0.7b Fruity 3.2a 3.1a Sour aromatic 1.1a 1.0a Cardboard/mushroom ND* 1.2 Fishy ND* ND* Sweet taste 8.5a 8.4a Sour taste 1.5a 1.3a Metallic 2.0a 2.2a Comments Flavor is muted but free of off flavors Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. *NDnot detected Means in a row followed by different letters are different (p < 0.05)

[0349] At 7 days, the yogurts in the light box are distinct in flavor profile from the dark control product. Flavor difference is due to light oxidized flavor. At 7 days, yogurts stored in the dark did not have fishy aroma and flavor.

TABLE-US-00021 TABLE 1c Sensory profile of yogurt at day 14 Dark Attribute control Light box Dairy case Aroma intensity 3.9a 3.3b 3.3b In-mouth Cooked/milky 3.7a 3.2b 3.2b Sweet aromatic/ 1.6a 1.0b 1.2b vanillin Fruity 3.0a 2.8a 2.8a Sour aromatic 1.1a 1.0a 1.0a Cardboard/mushroom ND* 1.2a 1.0a Fishy ND* 1.1 ND* Sweet taste 8.3a 8.4a 8.5a Sour taste 1.5a 1.4a 1.3a Metallic 2.0a 2.1a 2.2a Comments Aroma is Flavor is muted, low muted but intensity of fishy free of off flavors flavor in-mouth (7/7 panelists) Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. ND*not detected Means in a row followed by different letters are different (p < 0.05)

[0350] At 14 days, the yogurts in the light box have a distinct fishy flavor. Yogurts in the lighted dairy case are distinct in flavor profile from the dark control product. Flavor difference is due to light oxidized flavor. At 14 days, yogurts stored in the dark did not have fishy aroma and flavor.

TABLE-US-00022 TABLE 1d Sensory profile of yogurt at day 21 Dark Attribute control Light box Dairy case Aroma intensity 3.5a 3.0b 3.4a In-mouth Cooked/milky 3.5a 3.0b 3.4a Sweet aromatic/ 1.3a 1.0a 1.0a vanillin Fruity 3.0a 2.4b 2.8a Sour aromatic 1.0a 1.0a 1.0a Cardboard/mushroom ND* 1.0a 1.0a Fishy ND* 1.6 ND* Sweet taste 8.3a 8.4a 8.5a Sour taste 1.5a 1.5a 1.3a Metallic 2.0a 2.2a 2.2a Comments Visibly lighter in Slight visible color color, Aroma is difference, Flavor muted, low is muted, intensity 2/7 panelists of fishy consistently flavor in- detected mouth (7/7) fishy flavor Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. ND*not detected Means in a row followed by different letters are different (p < 0.05)

[0351] At 21 days, the yogurts in the light box have a distinct fishy flavor. Yogurts in the lighted dairy case are distinct in flavor profile from the dark control product. Flavor difference is due to light oxidized flavor. A few panelists detected fishy flavor in the yogurt stored in the lighted dairy case. At 21 days, yogurts stored in the dark did not have fishy aroma and flavor.

TABLE-US-00023 TABLE 1e Sensory profile of yogurt at day 28 Attribute Dark control Dairy case Aroma intensity 3.5a 3.1b Cooked/milky 3.5a 3.1b Sweet aromatic/vanillin 1.3a l.0b Fruity 3.0a 2.6b Sour aromatic 1.0a 1.0a Cardboard/mushroom ND* 1.3 Fishy ND* 0.5 Sweet taste 8.0a 8.2a Sour taste 1.5a 1.3a Metallic 2.0a 2.2a Comments Visibly lighter in color, Flavor is muted, 4/7 panelists consistently detected fishy flavor Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. ND*not detected Means in a row followed by different letters are different (p < 0.05)

[0352] At 28 days, yogurts in the lighted dairy case are distinct in flavor profile from the dark control product. Flavor difference is due to light oxidized flavor. More panelists detected fishy flavor in the yogurt stored in the lighted dairy case. At 28 days, yogurts stored in the dark did not have fishy aroma and flavor.

TABLE-US-00024 TABLE 1f Sensory profile of yogurt at day 42 Attribute Dark control Dairy case Aroma intensity 3.5a 2.8b In-mouth Cooked/milky 3.5a 2.8b Sweet aromatic/vanillin 1.5a 0.8b Fruity 3.2a 2.0b Sour aromatic 1.0a 1.0a Cardboard/mushroom ND* 1.9 Fishy ND* 1.5 Sweet taste 8.2a 8.5a Sour taste 1.5a 1.3a Metallic 2.2a 2.2a Comments Fishy/metallic aroma, visibly lighter in color, Flavor is muted, 7/7 panelists consistently detected fishy flavor Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. ND*not detected Means in a row followed by different letters are different (p < 0.05)

[0353] At 42 days, yogurts in the lighted dairy case have a distinct fishy aroma and flavor. At 42 days, yogurts stored in the dark did not have fishy aroma and flavor.

TABLE-US-00025 TABLE 1g Sensory profile of yogurt at day 56 Attribute Dark control Dairy case Aroma intensity 3.0a 2.2b In-mouth Cooked/milky 3.5a 2.0b Sweet aromatic/vanillin 1.5 ND* Fruity 3.2a 2.2b Sour aromatic 1.0a 1.0a Cardboard/mushroom ND* 2.2 Fishy ND* 1.8 Sweet taste 8.4a 8.5a Sour taste 1.7a 1.6a Metallic 2.0b 2.4a Comments Fishy/metallic aroma, visibly lighter in color, Flavor is muted, 7/7 panelists consistently detected fishy flavor Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. ND*not detected Means in a row followed by different letters are different (p < 0.05)

[0354] At 56 days, yogurts in the lighted dairy case have a distinct fishy aroma and flavor. At 56 days, yogurts stored in the dark did not have fishy aroma and flavor.

[0355] FIG. 1 shows the fishy flavor intensity in drinkable yogurts stored in a refrigerated lighted dairy case (700 Lx). At day 28, about half of the trained panel consistently detected fishy flavor in the yogurts. At day 42, all panelists consistently detected fishy flavor.

[0356] FIG. 2 shows the sensory profiles of yogurt stored at 4 C in the dark and at 4 C in a lighted dairy case (700 Lx) for 14 days. Sweet taste is not included on graphs since values were not different (p>0.05) and the higher scale intensity masks other differences. The blue line is the yogurt stored in dark. The organge line is the yogurt stored in the refrigerated lighted dairy case.

[0357] FIG. 3 shows the sensory profiles of yogurt stored at 4 C in the dark and at 4 C in a lighted dairy case (700 Lx) for 28 days. Sweet taste is not included on graphs since values were not different (p>0.05) and the higher scale intensity masks other differences. The blue line is the yogurt stored in dark. The organge line is the yogurt stored in the refrigerated lighted dairy case.

[0358] FIG. 4 shows the sensory profiles of yogurt stored at 4 C in the dark and at 4 C in a lighted dairy case (700 Lx) for 42 days. Sweet taste is not included on graphs since values were not different (p>0.05) and the higher scale intensity masks other differences. The blue line is the yogurt stored in dark. The organge line is the yogurt stored in the refrigerated lighted dairy case.

[0359] FIG. 5 shows the sensory profiles of yogurt stored at 4 C in the dark and at 4 C in a lighted dairy case (700 Lx) for 56 days. Sweet taste is not included on graphs since values were not different (p>0.05) and the higher scale intensity masks other differences. The blue line is the yogurt stored in dark. The organge line is the yogurt stored in the refrigerated lighted dairy case.

Example 10Sensory Panel Testing of Cherry Vanilla Yogurt and Yogurt Drinks

[0360] Cherry vanilla yogurt and cherry vanilla yogurt drinks were received April 5. Yogurts were stored at 4 C per the timepoint structure below.

TABLE-US-00026 April 8, T1 April 11, T2 April 15, T3 April 18 T4

[0361] Descriptive sensory and statistical analyses were performed as described in Example 9.

[0362] The yogurts tested were formulated as shown in the Table below.

TABLE-US-00027 Ingredient % of Formulation Whole Milk 70.24-89.9% Stabilizer Blend (Tapioca Starch, 1.2-3.03% Locust Bean Gum, Pectin, Agar) Non Fat Dry Milk 1.4-4.8% Flax Oil (50% ALA) .11-.95% Algal Oil (40% DHA) .11-.95% Choline Chloride (74% Choline) .05-.495% Culture .001-.04% Antioxidant .001-.04% Fruit Prep 9.95-19.95%

[0363] The yogurt drinks tested were formulated as shown in the Table below.

TABLE-US-00028 Ingredient % of Formulation Whole Milk 40-60.5% Tapioca Starch .1-2.05% Milk Protein Concentrate 1.2-3% Gellan Gum .01-1.05% Water 15.5-30% Algal Oil (40% DHA) .11-.95% Flax Oil (50% ALA) .11-.95% Choline Chloride (74% Choline) .05-.495% Antioxidant .001-.04% Culture .001-.04% Fruit Prep 9.95-19.95%

[0364] The results of sensory testing are shown in the following Tables.

TABLE-US-00029 TABLE 1 Sensory profile of drinkable yogurts across time Attribute T1 T2 T3 T4 Aroma intensity 4.3a 3.8b 3.6b 3.5b In-mouth 2.0 Cooked/milky 3.9a 3.3b 3.0b 2.5c Sweet aromatic/vanillin 1.3a 0.6b 0.6b ND* Fruity 3.6a 3.0b 3.1b 2.2c Sour aromatic 0.5a 0.5a 0.7a 1.0a Earthy/moldy ND* ND* ND* 2.5 Cardboard/mushroom ND* ND* ND* ND* Fishy ND* ND* ND* ND* Sweet taste 7.7a 8.0a 8.0a 7.5a Sour taste 1.2b 1.3b 1.3b 1.6a Astringent 2.5a 2.5a 2.3a 2.6a Metallic 1.8a 2.0a 2.0a 2.0a Comments chalky chalky chalky MOLDY, SPOILED Attributes are scored on a 0- to15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*not detected

TABLE-US-00030 TABLE 2 Sensory profile of yogurts across time Attribute T1 T2 T3 T4 Aroma intensty 2.4a 2.6a 2.6a 2.4a In-mouth Cooked/milky 4.5a 4.0b 4.0b 3.5b Sweet aromatic/vanillin 1.2a 1.4a 1.5a 1.5a Fruity 2.8a 3.0a 3.0a 3.2a Sour aromatic 1.7b 1.8b 1.7b 2.2a Cardboard/mushroom ND* ND* ND* ND* Fishy ND* ND* ND* ND* Sweet taste 6.5a 6.7a 6.6a 6.4a Sour taste 2.3a 2.2a 2.2a 2.5a Astringent 2.8a 2.8a 2.9a 3.0a Metallic 1.0b 1.3ab 1.4ab 1.6a comments chalky chalky chalky Chalky, more sour Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor atributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*not detected

Example 11Sensory Panel Testing of Strawberry Banana and Cherry Vanilla Yogurt Drinks

[0365] Strawberry banana and cherry vanilla yogurt drinks, formulated as shown in Example 10, were received May 22. Yogurts were stored at 4 C and sampled per the timepoint structure below.

TABLE-US-00031 May 24, T1 May 29, T2 June 3, T3 June 7 T4

[0366] Descriptive sensory and statistical analyses were performed as described in Example 9.

TABLE-US-00032 TABLE 1 Sensory profile of cherry vanilla drinkable yogurts across time Attribute T1 T2 T3 T4 Aroma intensity 3.8a 3.8a 3.6ab 3.5b In-mouth Cooked/milky 4.0a 3.8b 3.0b 3.1b Sweet aromatic/vanillin 1.5a 1.1b 0.8b ND* Fruity 3.8a 3.0b 3.1b 2.4c Sour aromatic 0.5b 0.8ab 0.7ab 1.0a Earthy/moldy ND* ND* ND* ND* Cardboard/mushroom ND* ND* ND* ND* Fishy ND* ND* ND* 1.5 Sweet taste 7.8a 7.7a 7.8a 7.5a Sour taste 1.5a 1.6a 1.4a 1.6a Astringent 2.5a 2.5a 2.7a 2.6a Metallic 1.9a 2.2a 2.3a 2.6a Comments Chalky, Chalky, Chalky, LOW FISHY low oily low oily low oily AROMA AND flavor flavor flavor FLAVOR Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*not detected

TABLE-US-00033 TABLE 2 Sensory profile of strawberry banana drinkable yogurts across time Attribute T1 T2 T3 T4 Aroma intensity 4.8a 4.5a 4.5a 4.5a In-mouth Cooked/milky 3.5a 3.5a 3.4a 3.4a Fruity/candy banana 3.4a 3.5a 3.2a 3.2a Fruity berry 1.5a 1.5a 1.2a 1.2a Sour aromatic 0.8a 0.8a 1.0a 1.0a Earthy/moldy ND* ND* ND* ND* Cardboard/mushroom ND* ND* ND* ND* Fishy ND* ND* ND* ND* Sweet taste 7.5a 7.6a 7.6a 7.7a Sour taste 1.5a 1.5a 1.6a 1.7a Astringent 2.5a 2.5a 2.7a 2.6a Metallic 2.2a 2.0a 2.0a 2.0a Comments Chalky, Chalky, Chalky, Chalky, low oily low oily low oily low oily flavor flavor flavor flavor Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*not detected

Example 12Sensory Panel Testing of Strawberry Banana and Cherry Vanilla Yogurt Drinks Under Lighted and Light-Shielded Conditions

[0367] Strawberry banana and cherry vanilla yogurt drinks, formulated as shown in Example 10 and provided in bottles and tubes, were received July 11. Products were assigned to a lighted dairy case or a dark light-shielded box. Yogurts were stored at 4 C and sampled per the timepoint structure below.

TABLE-US-00034 Lighted dairy case Aug 16 July 11, T1* July 19, T2 July 26 T3 Aug 2, T4 Aug 9 T5 T6 Light shielded (dark) July 11, July 19, July 26 Aug 2, Aug 9 Aug 16 Aug 23 Aug 30 T1* T2 T3 T4 T5 T6 T7 T8 Sept 6, Sept 13, T9 T10 *T1 is the same for both storage conditions. This timepoint occurred on receipt.

[0368] Descriptive sensory and statistical analyses were performed as described in Example 9.

TABLE-US-00035 TABLE 1a Sensory profile of mixed berry yogurts in bottles across time in the dark Attribute T1 T2 T3 T4 T5 T6 T7 Aroma 3.9a 3.8a 3.0b 3.0b 3.4ab 3.2b 3.5ab intensity In-mouth Cooked/ 3.7a 3.8a 3.5ab 3.3b 3.5ab 3.3b 3.3b milky Fruity 3.0a 3.0a 2.7a 2.9a 2.8a 2.7a 2.7a Sour 1.2b 1.0b 1.2b 1.2b 1.4a 1.4a 1.5a aromatic Earthy/ ND* ND* ND* ND* ND* ND* ND* moldy Cardboard/ ND* ND* ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* ND* ND* ND* ND* Sweet taste 5.7a 5.8a 5.5a 5.7a 5.8a 5.7a 5.6a Sour taste 2.8b 2.7b 2.6b 2.6b 2.9ab 3.0a 3.2a Astringent 2.9a 2.9a 2.8a 2.8a 3.0a 3.0a 3.0a Metallic 2.0a 2.2a 2.0a 2.0a 2.2a 2.2a 2.1a Comments Chalk- Chalk- Chalk- Chalk- chalk- chalk- chalk- y y y y y y y Attribute T8 T9 T10 Aroma 3.1b 3.2b 3.0b intensity In-mouth Cooked/ 3.3b 3.2b 3.3b milky Fruity 2.8a 2.8a 2.6a Sour 1.5a 1.4a 1.5a aromatic Earthy/ ND* ND* ND* moldy Cardboard/ ND* ND* ND* mushroom Fishy ND* ND* ND* Sweet taste 5.7a 5.6a 5.6a Sour taste 3.3a 3.3a 3.4a Astringent 3.0a 3.2a 3.2a Metallic 2.2a 2.2a 2.3a Comments chalk- Chalky, Chalky, y sour sour Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*not detected

TABLE-US-00036 TABLE 1b Sensory profile of mixed berry yogurts in bottles across time in a lighted dairy case Attribute T1 T2 T3 T4 T5 T6 Aroma intensity 3.9a 3.8a 3.0b 3.4ab 3.4ab 3.2b In-mouth Cooked/milky 3.7a 3.9a 3.4b 3.3b 3.5b 3.4b Fruity 3.0a 3.2a 2.8ab 2.9ab 2.8ab 2.6b Sour aromatic 1.2b 1.0b 1.4ab 1.2b 1.4ab 1.5a Earthy/moldy ND* ND* ND* ND* ND* ND* Cardboard/ ND* ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* ND* ND* ND* Sweet taste 5.7a 5.6a 5.4a 5.6a 5.8a 5.7a Sour taste 2.8a 2.7a 2.7a 2.7a 2.9a 3.0a Astringent 2.9a 2.7a 2.8a 2.9a 3.0a 3.0a Metallic 2.0a 2.2a 2.0a 2.0a 2.2a 2.2a Comments Chalky Chalky Chalky Chalky chalky chalky Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected NOTE: T1 is the same for both storage conditions as it was conducted at time of receipt.

TABLE-US-00037 TABLE 2a Sensory profile of strawberry yogurts in bottles across time in the dark Attribute T1 T2 T3 T4 T5 Aroma intensity 4.0a 3.8a 3.2b 3.0b 3.4b In-mouth Cooked/milky 3.8a 3.8a 3.5ab 3.3b 3.5ab Fruity 2.8a 3.0a 2.7a 2.9a 2.8a Sour aromatic 1.2b 1.4b 1.4b 1.5ab 1.6ab Vitamin 2.2a 2.4a 2.2a 2.2a 2.0a Cardboard/ ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* ND* ND* Sweet taste 5.5a 5.8a 5.7a 5.7a 5.8a Sour taste 3.3a 3.0b 3.2ab 3.2ab 3.2ab Astringent 2.9b 2.9b 2.9b 2.8b 3.1a Metallic 2.0a 2.2a 2.0a 2.0a 2.2a Comments Chalky Chalky Chalky Chalky chalky Attribute T6 T7 T8 T9 T10 Aroma intensity 3.4b 3.3b 3.3b 3.3b 3.3b In-mouth Cooked/milky 3.2b 3.3b 3.2b 3.2b 3.3b Fruity 2.7a 2.4b 2.7a 2.5ab 2.2b Sour aromatic 1.6ab 1.7a 1.8a 1.8a 1.7a Vitamin 2.2a 2.3a 2.4a 2.4a 2.3a Cardboard/ ND* ND* ND* 1.0 0.8 mushroom Fishy ND* ND* ND* ND* ND* Sweet taste 5.7a 5.6a 5.8a 5.7a 5.6a Sour taste 3.2ab 3.4a 3.5a 3.5a 3.6a Astringent 3.0ab 3.3a 3.4a 3.4a 3.3a Metallic 2.2a 2.1a 2.3a 2.3a 2.2a Comments chalky chalky chalky Chalky, Chalky, sour sour Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected

TABLE-US-00038 TABLE 2b Sensory profile of strawberry yogurts in bottles across time in a lighted dairy case Attribute T1 T2 T3 T4 T5 T6 Aroma intensity 4.0a 3.8a 3.2b 3.0b 3.2b 3.4b In-mouth Cooked/milky 3.8a 3.7a 3.6ab 3.3b 3.1b 3.0b Fruity 2.8a 2.9a 2.8a 3.0a 2.5b 2.3b Sour aromatic 1.2b 1.3b 1.5ab 1.7a 1.7a 1.7a Vitamin 2.2a 2.2a 2.1a 2.0a 2.2a 2.2a Cardboard/ ND* ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* ND* ND* ND* Sweet taste 5.5a 5.6a 5.7a 5.7a 5.8a 5.7a Sour taste 3.3a 3.2a 3.3a 3.3a 3.3a 3.3a Astringent 2.9a 2.9a 3.0a 2.8a 3.2a 3.0a Metallic 2.0a 2.2a 2.0a 2.2a 2.4a 2.5a Comments Chalky Chalky Chalky Chalky Chalky, Chalky, oily oily, muted flavor Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND *-not detected NOTE: T1 is the same for both storage conditions as it was conducted at time of receipt.

TABLE-US-00039 TABLE 3a Sensory profile of cherry vanilla yogurts in tubes across time in the dark Attribute T1 T2 T3 T4 T5 Aroma intensity 4.3a 3.8a 3.2b 3.0b 3.1b In-mouth Cooked/milky 4.0a 3.8a 3.7a 3.5ab 3.3b Sweet 2.7a 2.5a 2.5a 2.5a 2.4a aromatic/vanillin Fruity 3.8a 3.8a 3.4b 3.3b 2.8c Sour aromatic 0.8c 1.0bc 1.3b 1.3b 1.6a Cardboard/ ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* ND* ND* Sweet taste 6.4a 6.2a 6.0a 6.0a 6.2a Sour taste 2.0b 2.1b 2.3b 2.3b 2.2b Astringent 2.5ab 2.4b 2.8a 2.5ab 2.7a Metallic 2.0b 2.0b 2.0b 2.0b 2.1b Comments Attribute T6 T7 T8 T9 T10 Aroma intensity 3.4b 3.3b 3.1b 3.2b 3.0b In-mouth Cooked/milky 3.2b 3.0b 3.0b 3.0b 3.0b Sweet 2.5a 2.4ab 2.4ab 2.2b 2.1b aromatic/vanillin Fruity 3.0bc 2.6c 2.9c 2.5c 2.4c Sour aromatic 1.6a 1.8a 1.6a 1.6a 1.5a Cardboard/ ND* ND* ND* ND* ND* mushroom Fishy ND* ND* ND* 1.2a 1.3a Sweet taste 6.2a 6.2a 6.2a 6.0a 6.0a Sour taste 2.5a 2.4ab 2.6a 2.5a 2.7a Astringent 2.7a 2.9a 3.0a 3.0a 2.9a Metallic 2.1b 2.0b 2.3a 2.4a 2.5a Comments fishy fishy Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected

TABLE-US-00040 TABLE 3b Sensory profile of cherry vanilla yogurts in tubes across time in a lighted dairy case Attribute T1 T2 T3 T4 T5 T6 Aroma intensity 4.3a 3.8a 3.2b 3.3b 3.0b 3.0b In-mouth Cooked/milky 4.0a 3.9a 3.6a 3.5ab 3.1b 3.0b Sweet 2.7a 2.5a 2.6a 2.7a 2.2b 2.1b aromatic/vanillin Fruity 3.8a 3.8a 3.5ab 3.5ab 3.4ab 3.1b Sour aromatic 0.8b 1.0b 1.2b 1.1b 1.6a 1.7a Cardboard/mushroom ND* ND* ND* ND* ND* 1.2 Fishy ND* ND* ND* ND* ND* 1.0 Sweet taste 6.4a 6.3a 6.4a 6.3a 6.3a 6.3a Sour taste 2.0b 2.1b 2.2b 2.4ab 2.5a 2.6a Astringent 2.5a 2.5a 2.7a 2.5a 2.9a 2.7a Metallic 2.0b 2.0b 2.0b 2.0b 2.2b 2.6a Comments muted Fishy Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected NOTE: T1 is the same for both storage conditions as it was conducted at time of receipt.

TABLE-US-00041 TABLE 4a Sensory profile of strawberry banana yogurts in tubes across time in the dark Attribute T1 T2 T3 T4 T5 Aroma intensity 4.5a 3.9a 3.9a 4.2a 3.9a In-mouth Cooked/milky 3.5a 3.5a 3.4a 3.5a 3.5a Candy banana 3.0a 2.9a 2.8a 3.0a 2.9a Fruity 2.3a 2.2a 2.4a 2.3a 2.2a Vitamin 1.6a 1.6a 1.6a 1.6a 1.4a Sour aromatic 1.0b 1.0b 1.0b 1.0b 1.2ab Cardboard/mushroom ND* ND* ND* ND* ND* Fishy ND* ND* ND* ND* ND* Sweet taste 6.5a 6.6a 6.4a 6.6a 6.6a Sour taste 2.4a 2.5a 2.4a 2.5a 2.5a Astringent 2.5b 2.4b 2.4b 2.4b 2.4b Metallic 2.0b 2.0b 2.0b 2.0b 2.0b Comments Attribute T6 T7 T8 T9 T10 Aroma intensity 4.2a 3.5b 3.5b 3.3b 3.5b In-mouth Cooked/milky 3.3a 3.2a 3.2a 3.3a 3.3a Candy banana 2.7a 2.7a 2.6a 2.7a 2.6a Fruity 2.1ab 2.0b 2.0b 2.0b 1.9b Vitamin 1.6a 1.6a 1.4a 1.5a 1.7a Sour aromatic 1.4a 1.3ab 1.4a 1.4a 1.6a Cardboard/mushroom ND* ND* ND* ND* ND* Fishy ND* ND* ND* ND* 1.0 Sweet taste 6.5a 6.6a 6.6a 6.5a 6.6a Sour taste 2.5a 2.7a 2.6a 2.8a 2.7a Astringent 2.4b 2.7a 2.8a 2.8a 2.8a Metallic 2.0b 2.0b 2.0b 2.0b 2.4a Comments fishy Attributes are scored on a 0- to 15-point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected

TABLE-US-00042 TABLE 4b Sensory profile of strawberry banana yogurts in tubes across time in a lighted dairy case Attribute T1 T2 T3 T4 T5 T6 Aroma intensity 4.5a 3.9b 3.9b 3.7b 3.8b 3.8b In-mouth Cooked/milky 3.5a 3.6a 3.5a 3.5a 3.3ab 3.0b Candy banana 3.0a 2.7a 2.7a 2.9a 2.6ab 2.0b Fruity 2.0a 2.0a 2.4a 2.0a 2.0a 2.0a Vitamin 1.6a 1.4a 1.4a 1.6a 1.4a 1.7a Sour aromatic 1.0b 1.0b 1.1b 1.0b 1.5a 1.4a Cardboard/mushroom ND* ND* ND* 1.5 ND* ND* Fishy ND* ND* ND* ND* ND* 1.4 Sweet taste 6.5a 6.4a 6.5a 6.6a 6.6a 6.5a Sour taste 2.4a 2.5a 2.5a 2.5a 2.7a 2.7a Astringent 2.5a 2.4a 2.4a 2.4a 2.6a 2.7a Metallic 2.0b 2.0b 2.0b 2.0b 2.6a 2.5a Comments Muted, Fishy, Fishy, stale Color Color changed changed Attributes are scored on a 0 to 15 point universal Spectrum intensity scale. Most flavor attributes fall between 0 and 5. Means in a row followed by different letters are different (p < 0.05). ND*-not detected NOTE: T1 is the same for both storage conditions as it was conducted at time of receipt

Key Findings

[0369] 1. Mixed berry yogurts in bottles performed well across the storage time in the dark and in the lighted dairy case. Changes with storage were noted but were consistent between both storage conditions.

[0370] Strawberry yogurts in bottles performed well across the storage time in the dark and in the lighted dairy case. At the final two timepoints in the lighted case (days 35 and 42, T5 and T6), an oily note (not fishy) and muted overall flavor were noted.

[0371] Cherry vanilla yogurts in tubes performed well across the storage time in the dark through day 56. Low fishy flavors were detected at days 63 and 70. In the lighted dairy case, a low fishy flavor was detected at day 42 (T6).

[0372] Strawberry banana yogurts in tubes performed well across the storage time in the dark through day 63. A low fishy flavor was detected by day 70. In the lighted dairy case, a low fishy flavor was detected at days 35 and 42 (T5 and T6).

Example 13Preparation of Apple Sauce, Cinnamon Apple Sauce, and Strawberry Apple Sauce

[0373] Apple Sauce, cinnamon apple sauce and strawberry apple sauce were preparing by combining the ingredients in the table below.

TABLE-US-00043 TABLE 1 Apple Sauce % of Formulation by Ingredients weight Apple Base (e.g., 87.5-95% Apple puree 87.5-99% and 5.5-10% Apple puree concentrate) Omega-3 fatty acid powder 0.5-1.95%* Choline Chloride (74% Choline) 0.11-0.25% Mixed tocopherols (0.07%) (Antioxidant) 0.01-0.05% Rosemary extract 0.01-0.05%

TABLE-US-00044 TABLE 2 Cinnamon Apple Sauce % of Formulation by Ingredients weight Apple Cinnamon Base (e.g., 85-92.5% Apple 87.5-99% puree, 1.15-3.3% Banana puree, 4.5-11.5% Apple puree concentrate, and 0.01-0.25% Cinnamon) Omega-3 fatty acid powder 0.5-1.95%* Choline Chloride (74% Choline) 0.11-0.25% Mixed tocopherols (0.07%) (Antioxidant) 0.01-0.05% Rosemary extract 0.01-0.05%

TABLE-US-00045 TABLE 3 Strawberry Apple Sauce % of Formulation Ingredients by weight Strawberry Apple Base (e.g.; 68-81.5% Apple puree; 87.5-99% 10-25% Strawberry puree, 1.15-4.5% Banana puree, 0.15-1.25% Blackberry puree, and 10.75-19.5% Apple puree concentrate) Omega-3 fatty acid powder 0.5-1.95%* Choline Chloride (74% Choline) 0.11-0.25% Mixed tocopherols (0.07%) (Antioxidant) 0.01-0.05% Rosemary extract 0.01-0.05%

[0374] In some embodiments, the formulation given in the Table above can include 0.01-0.5% ascorbic acid.

[0375] The ingredients in Tables 1, 2, and 3 were purchased from commercial sources. The omega-3 fatty acid powder, at 1.10% provided between about 0.9 to about 1.6 mg DHA per gram of food product (here, apple sauce) and about 0.2 to about 0.5 mg EPA per gram of food product. The ingredients were weighed and mixed in a mixer to prepare puree A. The pH of puree A was determined to ensure that the pH was below 4.0 or below. Next, puree A was heated to a temperature of between 190 F.-193 F. and hot filled into pouches. The filled pouches were held at 190 F. for 5 minutes to prepare the apple sauce. The Brix value of the apple base (apple puree) was greater than 12. The omega-3 fatty acid powder is an encapsulated fatty acids that provides both DHA and EPA. The powder is about 30% polyunsaturated fatty acids (PUFA) by dry weight. Thus 1.1 grams of the omega-3 fatty acid powder provides about 330 mg of PUFAs per 100 grams of apple sauce. The amount of choline chloride, 0.18%, provides between about 180 mg of choline chloride per 100 grams of apple sauce, and about 130 mg of choline per 100 grams of the apple sauce (depending on processing losses). Rosemary extract is a natural preservative.

Example 14Accelerated Shelf Life Study of the Apple Sauces of Example 1

[0376] The apple sauces of example 1 were tested in accelerated shelf life studies. The apple sauces were prepared and hot filled into pouches exposed to 70 F. and 90 F. for 16 weeks and evaluated organoleptically at day 0, and weeks 3, 6, 9 and 16. The Table below provides the results.

TABLE-US-00046 Product name: Original-Apple sauce, Apple Cinnamon, Apple strawberry Description: Sensory Evaluation Start Date: Mar. 1, 2019 Product storage temperature: 70 F.-considered Ambient Storage. 90 F.-Hot Box (2x accelerated) Analysis Organoleptic Organoleptic Organoleptic Organoleptic Organoleptic Evaluation- Evaluation- Evaluation- Evaluation- Evaluation- Appearance Aroma Flavor Texture Conclusion Method Sensory evaluation Sensory evaluation Sensory evaluation Sensory evaluation Sensory evaluation comparing product comparing product comparing product comparing product comparing product to control sample to control sample to control sample to control sample to control sample (stored at (stored at (stored at (stored at (stored at refrigeration refrigeration refrigeration refrigeration refrigeration temperatures) temperatures) temperatures) temperatures) temperatures) Scale 1-very different 1-very 1-very 1-very from control/ different different different not acceptable from control/ from control/ from control/ 5-same as control not acceptable not acceptable not acceptable Sample Storage Sample name Week conditions Results Original-Apple sauce Day 0 70 F. Light-applesauce Fresh apple aroma Fresh apple flavor, Pulpy, runny Baseline color no offnotes Apple Cinnamon Day 0 70 F. Brown specs of Fresh apple aroma Fresh apple flavor, Pulpy, runny Baseline cinnamon strong cinnamon, no offnotes Apple strawberry Day 0 70 F. Pale pink color Fresh apple aroma Fresh apple flavor, Pulpy, runny Baseline slight tartness from strawberry, no offnotes Original-Apple sauce Week 3 90 F. 5 5 5 5 Acceptable Apple Cinnamon Week 3 90 F. 5 5 5 5 Acceptable Apple strawberry Week 3 90 F. 4 5 5 5 Acceptable Original-Apple sauce Week 6 90 F. 5 5 5 5 Acceptable Apple Cinnamon Week 6 90 F. 5 5 5 5 Acceptable Apple strawberry Week 6 90 F. 4 5 5 5 Acceptable Original-Apple sauce Week 9 90 F. 5 5 5 5 Acceptable Original-Apple sauce Week 9 70 F. 5 5 5 5 Acceptable Apple Cinnamon Week 9 90 F. 5 5 5 5 Acceptable Apple Cinnamon Week 9 70 F. 5 5 5 5 Acceptable Apple strawberry Week 9 90 F. 4-Notice color 5 4-Flavor slightly 5 Acceptable changes acidic as compared to control Apple strawberry Week 9 70 F. 5 5 5 5 Acceptable Original-Apple sauce Week 16 90 F. 4 5 5 5 Acceptable Original-Apple sauce Week 16 70 F. 5 5 5 5 Acceptable Apple Cinnamon Week 16 90 F. 5 5 5 5 Acceptable Apple Cinnamon Week 16 70 F. 5 5 5 5 Acceptable Apple strawberry Week 16 90 F. 3-Sample color 4 4-Flavor dull. 5 Not Acceptable. brown No noticeable Color and flavor strawberry both were affected significantly.