NOVEL COPOLYMER

Abstract

A copolymer in which a target-affinity molecule is bonded to a copolymer X having structural units of (A), (B), and (C):

##STR00001## R.sup.1, R.sup.2, and R.sup.3 are the same or different and are hydrogen or C.sub.1-3 alkyl. R.sup.4 is C.sub.1-3 alkyl. R.sup.5 is hydrogen, C.sub.1-18 alkyl, 3- to 8-membered cycloalkyl optionally having a substituent, adamantyl, C.sub.6-18 aryl optionally having a substituent, or a 5- to 10-membered heteroaryl optionally having a substituent. X.sup.1, X.sup.2, and X.sup.3 are the same or different and are oxygen, sulfur, or NR.sup.7. R.sup.6 is hydrogen, a leaving group, or a linker. R.sup.7 is hydrogen or C.sub.1-3 alkyl. m is a number of 1 to 100, and n is 0 to 3.

Claims

1. A copolymer, comprising: a copolymer X; and a target-affinity molecule bonded to the copolymer X, wherein the copolymer X comprises structural units of formulas (A), (B), and (C), ##STR00032## where, R.sup.1, R.sup.2, and R.sup.3 are the same or different and are a hydrogen atom or a C.sub.1-3 alkyl group, R.sup.4 is a C.sub.1-3 alkyl group, R.sup.5 is a hydrogen atom, a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, X.sup.1, X.sup.2, and X.sup.3 are the same or different and are an oxygen atom, a sulfur atom, or NR.sup.7, R.sup.6 is a hydrogen atom, a leaving group, or a linker, R.sup.7 is a hydrogen atom or a C.sub.1-3 alkyl group, m is an integer of 1 to 100, and n is an integer of 0 to 3.

2. The copolymer according to claim 1, wherein the copolymer X is a copolymer formed by polymerization of three types of monomers of formulas (1) to (3), ##STR00033##

3-18. (canceled)

19. The copolymer according to claim 1, wherein the copolymer has a number average molecular weight of 5,000 to 150,000.

20. The copolymer according to claim 1, wherein the target-affinity molecule is biotin or a derivative thereof.

21. The copolymer according to claim 1, wherein the target-affinity molecule is a molecule having a biotin residue of formula (a), ##STR00034## where Y is O or NH.

22. The copolymer according to claim 1, wherein the target-affinity molecule is at least one of molecules of formulas (8) to (11), ##STR00035## ##STR00036##

23. The copolymer according to claim 1, wherein the bond of the target-affinity molecule to the copolymer X is a covalent bond or a non-covalent bond.

24. A single chain nanoparticle, comprising: the copolymer of claim 1.

25. A pharmaceutical composition, comprising: the copolymer of claim 1.

26. A pre-targeting method, comprising: administering the pharmaceutical composition of claim 25.

27. The copolymer according to claim 2, wherein the copolymer has a number average molecular weight of 5,000 to 150,000.

28. The copolymer according to claim 2, wherein the target-affinity molecule is biotin or a derivative thereof.

29. The copolymer according to claim 2, wherein the target-affinity molecule is a molecule having a biotin residue of formula (a), ##STR00037## where Y is O or NH.

30. The copolymer according to claim 2, wherein the target-affinity molecule is any one of molecules of formulas (8) to (11), ##STR00038## ##STR00039##

31. The copolymer according to claim 2, wherein the bond of the target-affinity molecule to the copolymer X is a covalent bond or a non-covalent bond.

32. A single chain nanoparticle, comprising: the copolymer of claim 2.

33. A pharmaceutical composition, comprising: the copolymer of claim 2.

34. A pre-targeting method, comprising: administering the pharmaceutical composition of claim 33.

Description

BRIEF DESCRIPTION OF DRAWINGS

[0049] FIG. 1 is a diagram showing a 1H-NMR spectrum of a copolymer obtained in Example 1 measured by using nuclear magnetic resonance (NMR).

[0050] FIG. 2 is a diagram showing a chromatogram of a copolymer obtained in Example 1 obtained by gel permeation chromatography (GPC).

[0051] FIG. 3 is a diagram showing a particle diameter measurement result (scattering intensity distribution) in dynamic light scattering (DLS) for a copolymer before encapsulating DACHPt (Example 69) and a DACHPt-encapsulating SCNP (Example 70).

[0052] FIG. 4 is a diagram showing a 1H-NMR spectrum of a biotin-bonded SCNP obtained in Example 73, measured by using the nuclear magnetic resonance (NMR).

[0053] FIG. 5 is a diagram showing a chromatogram of the biotin-bonded SCNP obtained in Example 73 obtained by the gel permeation chromatography (GPC).

[0054] FIG. 6 is a diagram showing a particle diameter measurement result (scattering intensity distribution) of the biotin-bonded SCNP obtained in Example 73 in dynamic light scattering (DLS).

[0055] FIG. 7 is a diagram showing a 1H-NMR spectrum of a biotin-bonded SCNP obtained in Example 75, measured by using the nuclear magnetic resonance (NMR).

[0056] FIG. 8 is a diagram showing a chromatogram of the biotin-bonded SCNP obtained in Example 75 obtained by the gel permeation chromatography (GPC).

[0057] FIG. 9 is a diagram showing a particle diameter measurement result (scattering intensity distribution) of the biotin-bonded SCNP obtained in Example 75 in dynamic light scattering (DLS).

[0058] FIG. 10 is a diagram showing a 1H-NMR spectrum of a biotin-bonded SCNP obtained in Example 77, measured by using the nuclear magnetic resonance (NMR).

[0059] FIG. 11 is a diagram showing a chromatogram of the biotin-bonded SCNP obtained in Example 77 obtained by the gel permeation chromatography (GPC).

[0060] FIG. 12 is a diagram showing a particle diameter measurement result (scattering intensity distribution) of the biotin-bonded SCNP obtained in Example 77 in dynamic light scattering (DLS).

[0061] FIG. 13 is a diagram showing a 1H-NMR spectrum of a biotin-bonded SCNP obtained in Example 79, measured by using the nuclear magnetic resonance (NMR).

[0062] FIG. 14 is a diagram showing a chromatogram of the biotin-bonded SCNP obtained in Example 79 obtained by the gel permeation chromatography (GPC).

[0063] FIG. 15 is a diagram showing a particle diameter measurement result (scattering intensity distribution) of the biotin-bonded SCNP obtained in Example 79 in dynamic light scattering (DLS).

[0064] FIG. 16 is a diagram showing a change in relative tumor volume when an oxaliplatin solution or the DACHPt-encapsulating SCNP (Example 70) was administered 3 times every other day to a model mouse having a back subcutaneously transplanted with a mouse colon cancer cell line (C26).

DESCRIPTION OF EMBODIMENTS

[0065] Terms in the present description are used in the meanings commonly used in the field unless otherwise specified. Hereinafter, the present invention will be described in more detail.

[0066] In the present description, the term nanoparticle refers to a structure showing a particle diameter of 100 nm or less.

[0067] In the present description, the term single chain nanoparticle (SCNP) refers to a nanoparticle formed by using, for example, chemical crosslinking, hydrophobic interaction, or ionic bonding in a single chain as a driving force. The SCNP often indicates a nanoparticle having a relatively small particle diameter of 20 nm or less among nanoparticles.

[0068] In the present description, the term initiator means an initiator for thermal radical polymerization such as an azo compound or a peroxide.

[0069] In the present description, the term chain transfer agent refers to a compound that causes a chain transfer reaction in radical polymerization, and is preferably a compound having a thiocarbonyl group.

[0070] In the present description, the term C.sub.1-3 alkyl group means a linear or branched alkyl group having 1 to 3 carbon atoms, and examples thereof include a methyl group, an ethyl group, an n-propyl group, and an isopropyl group.

[0071] In the present description, the term C.sub.1-18 alkyl group means a linear or branched, alkyl group having 1 to 18 carbon atoms, and examples thereof include a methyl group, an ethyl group, an n-propyl group, an isopropyl group, an n-butyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, a pentyl group, a hexyl group, a heptyl group, an octyl group, a nonyl group, a decyl group, an undecyl group, a dodecyl group, a tridecyl group, a tetradecyl group, a pentadecyl group, a hexadecyl group, a heptadecyl group, and an octadecyl group.

[0072] In the present description, the term 3- to 8-membered cycloalkyl group optionally having a substituent means a cyclic alkyl group having 3 to 8 carbon atoms, and examples thereof include a cyclopropyl group, a cyclobutyl group, a cyclopentyl group, a cyclohexyl group, a cycloheptyl group, and a cyclooctyl group. The substituent is not particularly limited, and examples thereof include a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, an alkynyl group having 2 to 6 carbon atoms, a hydroxyl group, an alkoxy group having 1 to 6 carbon atoms, an amino group, an alkylamino group having 1 to 6 carbon atoms, a di-alkylamino group having 1 to 6 carbon atoms in which alkyl groups are the same or different, a thiol group, an alkylthio group having 1 to 6 carbon atoms, a carboxyl group, an alkoxycarbonyl group having 1 to 6 carbon atoms, and a carbamoyl group.

[0073] In the present description, the term C.sub.6-18 aryl group optionally having a substituent means a monocyclic or polycyclic condensed aromatic hydrocarbon group, and examples thereof include a phenyl group, a naphthyl group, an anthracenyl group, a phenanthrenyl group, a triphenylenyl group, a pyrenyl group, a chrysenyl group, and a naphthacenyl group. Further, a C.sub.6-14 aryl group optionally having a substituent means a monocyclic or polycyclic condensed aromatic hydrocarbon group, and examples thereof include a phenyl group, a naphthyl group, an anthracenyl group, and a phenanthrenyl group. The substituent is not particularly limited, and examples thereof include a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, an alkynyl group having 2 to 6 carbon atoms, a hydroxyl group, an alkoxy group having 1 to 6 carbon atoms, an amino group, an alkylamino group having 1 to 6 carbon atoms, a di-alkylamino group having 1 to 6 carbon atoms in which alkyl groups are the same or different, a thiol group, an alkylthio group having 1 to 6 carbon atoms, a carboxyl group, an alkoxycarbonyl group having 1 to 6 carbon atoms, and a carbamoyl group.

[0074] In the present description, the term 5- to 10-membered heteroaryl group optionally having a substituent means a 5- to 10-membered, monocyclic aromatic heterocyclic group or condensed aromatic heterocyclic group containing 1 to 4 heteroatoms selected from a nitrogen atom, an oxygen atom, and a sulfur atom, other than a carbon atom, as atoms constituting the ring. Examples of the monocyclic aromatic heterocyclic group include a furyl group, a thienyl group, a pyrrolyl group, a pyridinyl group, a pyrazinyl group, a pyrimidinyl group, a pyridazinyl group, an imidazolyl group, a pyrazyl group, a thiallyl group, an oxazolyl group, an isoxazolyl group, a 1,3,4-thiadiazolyl group, a 1, 2, 3-triazolyl group, a 1,2,4-triazolyl group, and a tetrazolyl group. Examples of the condensed aromatic heterocyclic group include a benzofuranyl group, a benzothiophenyl group, a quinoxalinyl group, an indolyl group, an isoindolyl group, an isobenzofuranyl group, a chromanyl group, a benzimidazolyl group, a benzothiazolyl group, a benzoxazolyl group, a quinolyl group, and an isoquinolinyl group. The term 6- to 10-membered heteroaryl group optionally having a substituent means a 6- to 10-membered, monocyclic aromatic heterocyclic group or condensed aromatic heterocyclic group containing 1 to 4 heteroatoms selected from a nitrogen atom, an oxygen atom, and a sulfur atom, other than a carbon atom, as the atoms constituting the ring. Examples of the monocyclic aromatic heterocyclic group include a pyridinyl group, a pyrazinyl group, a pyrimidinyl group, and a pyridazinyl group. Examples of the condensed aromatic heterocyclic group include a benzofuranyl group, a benzothiophenyl group, a quinoxalinyl group, an indolyl group, an isoindolyl group, an isobenzofuranyl group, a chromanyl group, a benzimidazolyl group, a benzothiazolyl group, a benzoxazolyl group, a quinolyl group, and an isoquinolinyl group. The substituent is not particularly limited, and examples thereof include a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, an alkynyl group having 2 to 6 carbon atoms, a hydroxyl group, an alkoxy group having 1 to 6 carbon atoms, an amino group, an alkylamino group having 1 to 6 carbon atoms, a di-alkylamino group having 1 to 6 carbon atoms in which alkyl groups are the same or different, a thiol group, an alkylthio group having 1 to 6 carbon atoms, a carboxyl group, an alkoxycarbonyl group having 1 to 6 carbon atoms, and a carbamoyl group.

[0075] In the present description, examples of the halogen atom include a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom.

[0076] In the present description, the term target-affinity molecule means biotin or a derivative thereof having a specific binding function to avidin/streptavidin or a mutant (modified) form or functional fragment thereof. The target-affinity molecule may be carried on the copolymer by an action such as an electrostatic interaction, a hydrogen bond, a hydrophobic interaction, or a covalent bond to the copolymer X of the present invention.

[0077] In the present description, the term pre-targeting method means a drug delivery method for accurately accumulating a drug on a target cell such as a cancer cell. First, a fusion molecule probe such as a cancer antigen-specific antibody molecule and avidin/streptavidin or a mutant (modified) form or functional fragment thereof is prepared, and administered to a patient to accumulate the fusion molecule on the cancer cell. Next, a process is performed to administer a conjugate of a pharmaceutical composition and a target-affinity molecule such as biotin having affinity for the avidin/streptavidin or the mutant (modified) form or functional fragment thereof to a patient, thereby accurately accumulating the drug on the cancer cell.

[0078] It is also possible to prepare a complex in which a conjugate of the molecular probe such as the cancer antigen-specific antibody molecule and the avidin/streptavidin or the mutant (modified) form or functional fragment thereof is bonded to a conjugate of that target-affinity molecule and the pharmaceutical composition in advance and administer the complex to the patient.

[0079] In the present description, the term pharmaceutical composition means one in which an active ingredient (drug, or physiologically active substance) that can be used for diagnosis, prevention, or treatment of a disease is carried on the copolymer X or the target-affinity copolymer of the present invention by the action such as the electrostatic interaction, the hydrogen bond, the hydrophobic interaction, or the covalent bond. Examples of the carrying form include a form in which the drug is present on the particle surface, a form in which the drug is contained in the nanoparticle, and a combination form thereof where the copolymer X or the target-affinity copolymer forms the nanoparticle.

[0080] One embodiment of the present invention is a copolymer (target-affinity copolymer) in which a target-affinity molecule is bonded to a copolymer X comprising structural units represented by the following formulas (A), (B), and (C).

##STR00009## [0081] wherein, R.sup.1, R.sup.2, and R.sup.3 are the same or different and represent a hydrogen atom or a C.sub.1-3 alkyl group, R.sup.4 represents a C.sub.1-3 alkyl group, R.sup.5 represents a hydrogen atom, a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, X.sup.1, X.sup.2, and X.sup.3 are the same or different and represent an oxygen atom, a sulfur atom, or NR.sup.7, R.sup.6 represents a hydrogen atom, a leaving group, or a linker, R.sup.7 represents a hydrogen atom or a C.sub.1-3 alkyl group, m represents an integer of 1 to 100, and n represents an integer of 0 to 3.

[0082] In the copolymer X of the present invention, the structural unit (A) functions as a unit that imparts hydrophilicity, and the structural unit (B) functions as a unit that imparts hydrophobicity. Further, the structural unit (C) functions as a scaffold in which the active ingredient (drug, or physiologically active substance) is bonded to the copolymer X or the target-affinity copolymer. Having these three structural units serves to imparting the copolymer X or the target-affinity copolymer a property of forming SCNP in water, and to facilitating the formed SCNP to precisely control particle diameter at a minute scale of 20 nm or less, and to function as a drug delivery carrier having high tumor accumulation.

[0083] R.sup.1 in the structural unit (A) represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0084] X.sup.1 represents an oxygen atom, the sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0085] m represents an integer of 1 to 100, is preferably an integer of 3 to 100, and from a viewpoint of imparting good hydrophilicity, preferably 3 to 80, more preferably 4 to 60, still more preferably 4 to 40, and even more preferably 4 to 22.

[0086] R.sup.4 represents a C.sub.1-3 alkyl group, specifically a methyl group, an ethyl group, an n-propyl group or an isopropyl group, preferably a methyl group or an ethyl group, and more preferably a methyl group.

[0087] R.sup.2 in the structural unit (B) represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0088] X.sup.2 represents an oxygen atom, a sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0089] n represents an integer of 0 to 3, preferably an integer of 1 to 3, and more preferably 1.

[0090] R.sup.5 represents a hydrogen atom, a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, and from a viewpoint of imparting the hydrophobicity to the structural unit (B), preferably a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, more preferably a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, and still more preferably a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group, an adamantyl group, or a C.sub.6-18 aryl group. Meanwhile, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-14 aryl group optionally having a substituent, or a 6- to 10-membered heteroaryl group optionally having a substituent is also preferable.

[0091] Here, the substituent is preferably one or more types selected from a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, and an alkynyl group having 2 to 6 carbon atoms.

[0092] R.sup.3 in the structural unit (C) represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0093] X.sup.3 represents an oxygen atom, a sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0094] R.sup.6 represents a hydrogen atom, a leaving group, or a linker. The leaving group is a group that can detach when the structural unit (C) binds to the drug (physiologically active substance), and the linker is a group that can be used for crosslinking when the structural unit (C) binds to the drug (physiologically active substance). As the leaving group or linker, a C.sub.1-18 alkyl group optionally having a substituent, a 3- to 8-membered cycloalkyl group optionally having a substituent, or a C.sub.7-19 aralkyl group optionally having a substituent is preferable. Here, examples of the substituent include a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, an alkynyl group having 2 to 6 carbon atoms, a hydroxyl group, an alkoxy group having 1 to 6 carbon atoms, an amino group, an alkylamino group having 1 to 6 carbon atoms, a di-alkylamino group having 1 to 6 carbon atoms in which alkyl groups are the same or different, a thiol group, an alkylthio group having 1 to 6 carbon atoms, a carboxyl group, an alkoxycarbonyl group having 1 to 6 carbon atoms, and a carbamoyl group. Among these groups, the linker is preferably a group having a functional group such as a hydroxyl group, an amino group, a thiol group, or a carboxyl group as a substituent.

[0095] Preferred specific examples of the leaving group of R.sup.6 include a group represented by the following formula (4):

##STR00010##

[0096] Preferred specific examples of the linker of R.sup.6 include groups selected from the following formulas (5) to (7).

##STR00011##

[0097] No text.

[0098] The copolymer X of the present invention is the copolymer having the structural units represented by the formulas (A), (B) and (C). The copolymer X may be a random copolymer or a block copolymer, and is preferably a random copolymer. As for a composition ratio of each structural unit in one molecule, when (A) is 1 part by mass, preferably (B) is 0.01 to 100 parts by mass and (C) is 0.1 to 100 parts by mass; more preferably (B) is 0.05 to 18 parts by mass and (C) is 0.1 to 20 parts by mass; and particularly preferably (B) is 0.05 to 4 parts by mass and (C) is 0.1 to 16 parts by mass.

[0099] A polymerization degree of the copolymer X of the present invention is not particularly limited, and it is preferably 5 000 to 150 000, and more preferably 8 000 to 150 000 as a number average molecular weight.

[0100] In the copolymer of the present invention, as described above, the monomer represented by general formula (1) functions as a unit that imparts the hydrophilicity, and the monomer represented by general formula (2) functions as a unit that imparts the hydrophobicity. Further, the monomer represented by general formula (3) functions as a scaffold to which the drug and the copolymer are bonded. Examples of the monomer functioning as the hydrophobic unit represented by general formula (2) include monomers represented by following formulas.

##STR00012## ##STR00013## ##STR00014## ##STR00015## ##STR00016## ##STR00017##

[0101] No text.

[0102] In general formula (1), R.sup.1 represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0103] In general formula (2), R.sup.2 represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0104] In general formula (3), R.sup.3 represents a hydrogen atom or a C.sub.1-3 alkyl group, and is preferably a hydrogen atom or a methyl group, preferably a hydrogen atom, an ethyl group, or a propyl group, and more preferably a hydrogen atom.

[0105] In general formula (1), R.sup.4 represents a C.sub.1-3 alkyl group, specifically a methyl group, an ethyl group, an n-propyl group or an isopropyl group, preferably a methyl group or an ethyl group, and more preferably a methyl group.

[0106] In general formula (1), X.sup.1 represents an oxygen atom, a sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0107] In general formula (1), m represents an integer of 1 to 100, and is preferably an integer of 3 to 100, and, preferably 3 to 80, more preferably 4 to 60, still more preferably 4 to 40, and still more preferably 4 to 22 from the viewpoint of imparting good hydrophilicity.

[0108] In general formula (2), R.sup.5 represents a hydrogen atom, a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent, and from the viewpoint of imparting hydrophobicity to the structural unit (B), a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent is preferable, a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-18 aryl group optionally having a substituent, or a 5- to 10-membered heteroaryl group optionally having a substituent is more preferable, and a C.sub.1-18 alkyl group, a 3- to 8-membered cycloalkyl group, an adamantyl group, or a C.sub.6-18 aryl group is still more preferable. In addition, a 3- to 8-membered cycloalkyl group optionally having a substituent, an adamantyl group, a C.sub.6-14 aryl group optionally having a substituent, or a 6- to 10-membered heteroaryl group optionally having a substituent is also preferable. Here, the substituent is preferably one or more types selected from a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, and an alkynyl group having 2 to 6 carbon atoms.

[0109] In general formula (2), X.sup.2 represents an oxygen atom, a sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0110] In general formula (2), n represents an integer of 0 to 3, preferably an integer of 1 to 3, and more preferably 1.

[0111] In general formula (3), R.sup.6 represents a hydrogen atom, a leaving group, or a linker. As the leaving group or linker, a C.sub.1-18 alkyl group optionally having a substituent, a 3- to 8-membered cycloalkyl group optionally having a substituent, or a C.sub.7-19 aralkyl group optionally having a substituent is preferable. Here, examples of the substituent include a halogen atom, an alkyl group having 1 to 6 carbon atoms, an alkenyl group having 2 to 6 carbon atoms, an alkynyl group having 2 to 6 carbon atoms, a hydroxyl group, an alkoxy group having 1 to 6 carbon atoms, an amino group, an alkylamino group having 1 to 6 carbon atoms, a di-alkylamino group having 1 to 6 carbon atoms in which alkyl groups are the same or different, a thiol group, an alkylthio group having 1 to 6 carbon atoms, a carboxyl group, an alkoxycarbonyl group having 1 to 6 carbon atoms, and a carbamoyl group. Among these groups, as the linker, a group having a functional group such as a hydroxyl group, an amino group, a thiol group, or a carboxyl group as a substituent is preferable.

[0112] Preferred specific examples of the leaving group of R.sup.6 include a group represented by the following formula (4):

##STR00018##

[0113] Preferred specific examples of the linker of R.sup.6 include groups selected from the following formulas (5) to (7).

##STR00019##

[0114] No text.

[0115] In general formula (3), X.sup.3 represents an oxygen atom, a sulfur atom, or NR.sup.7, and is preferably an oxygen atom, a sulfur atom, or NH, and more preferably an oxygen atom.

[0116] The copolymer X of the present invention is formed by copolymerizing three monomers represented by general formulas (1) to (3). The copolymerization may be random copolymerization or block copolymerization, and those formed by random copolymerization are preferable. As for a blending ratio of the three monomers, it is preferable that 0.01 to 100 parts by mass of monomer (2) and 0.1 to 100 parts by mass of monomer (3) are polymerized, it is more preferable that 0.05 to 18 parts by mass of monomer (2) and 0.1 to 20 parts by mass of monomer (3) are polymerized, and it is particularly preferable that 0.05 to 4 parts by mass of monomer (2) and 0.1 to 16 parts by mass of monomer (3) are polymerized, with respect to 1 part by mass of monomer (1).

[0117] In addition, solvates in which various solvents are coordinated are also included in the copolymer X of the present invention. In the present description, examples of the solvate include a hydrate and an ethanolate. The solvent may be coordinated to the copolymer X of the present invention in any number.

[0118] The copolymer X of the present invention can be produced by various known methods. The production method is not particularly limited, and for example, the copolymer X can be produced according to a basic polymer synthesis method described below.

##STR00020## [0119] wherein R represents a hydrogen atom or a C.sub.1-3 alkyl group, and R represents a group represented by R.sup.4, R.sup.5 or R.sup.6.

[0120] This reaction shows a step of producing a polymer (III) by reacting a monomer (I) with a chain transfer agent (II) and an initiator. This reaction can be performed in the absence of a solvent or in a solvent such as alcohols such as methanol, ethanol, 1-propanol, and 2-propanol; ethers such as diethyl ether, tetrahydrofuran, and 1,4-dioxane; aromatic hydrocarbons such as benzene, toluene, and xylene; halogenated hydrocarbons such as dichloromethane, chloroform, and 1,2-dichloroethane; N,N-dimethylformamide; N,N-dimethylacetamide; N-methylpyrrolidone; acetonitrile; and ethyl acetate, and it is preferable to use aromatic hydrocarbons such as toluene and xylene as a solvent.

[0121] As the chain transfer agent, it is possible to use 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAT), cyanomethyl dodecyltrithiocarbonate (CDTTC), 2-cyano-2-propyldodecyl trithiocarbonate (CPDTTC), 4-cyano-4-[(dodecylsulfanyl-thiocarbonyl)sulfanyl]pentanoic acid (CDSPA), 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid 3-azido-1-propanol ester (N.sub.3-CTA), and it is preferable to use DDMAT or N.sub.3-CTA, and more preferably N.sub.3-CTA. Where polymerization is carried out by using a chain transfer agent, the copolymer X of the present invention has a structure in which a part or all of a structure of the chain transfer agent is partially bonded. Where the copolymer X includes a structure of a chain transfer agent, the structure may be removed by an appropriate method.

[0122] As the initiator, an azo polymerization initiator such as 2,2-azobis-isobutyronitrile (AIBN), 1,1-azobis(cyclohexanecarbonitrile) (ACHN), 2,2-azobis-2-methylbutyronitrile (AMBN), 2,2-azobis-2,4-dimethylvaleronitrile (ADVN) can be used, and AIBN is preferably used.

[0123] The reaction temperature is 0 to 300 C., preferably 0 to 150 C., and more preferably 1 to 100 C., and the reaction time is 1 minute to 48 hours, and preferably 5 minutes to 24 hours. In this reaction, a random copolymerized copolymer X can be produced by carrying out the reaction in the coexistence of monomers (I) having different structures.

[0124] For example, if the N.sub.3-CTA having an azide group is used as the chain transfer agent, the copolymer X having an end at which the azide group is present will be obtained, which is advantageous for production of the target-affinity copolymer by a click reaction described later.

[0125] The target-affinity molecule bonded to the copolymer X is biotin or the derivative thereof having a specific binding function to avidin/streptavidin or the mutant (modified) form or functional fragment thereof.

[0126] Examples of biotin or the derivative thereof include biotin, norbiotin, homobiotin, iminobiotin, oxybiotin, thiobiotin, ethylbiotin, desthiobiotin, diaminobiotin, biotin carbamate, biotin carbonate, trisnorbiotin sulfoxide, bisnorbiotin sulfoxide, norbiotin sulfoxide, biotin sulfoxide, homobiotin sulfoxide, bishomobiotin sulfoxide, trishomobiotin sulfoxide, biotin sulfone, reduced biotin (biotinol), and compounds described in JP S58-154508 A and WO 2014/129446 A. Further, biocytin or a compound having a spacer as described in Avidin-Biotin Technical Handbook from Thermo Fisher can also be used. Moreover, examples of a bis-biotin derivative having high affinity for a streptavidin mutant having reduced affinity for natural biotin include compounds described in WO 2015/125820 A, WO 2018/151301 A, WO 2019/189867 A, WO 2019/230905 A, and WO 2021/210573 A, and are not limited thereto.

[0127] Here, biotin or the derivative thereof is preferably a molecule having a biotin residue represented by the following formula (a):

##STR00021## [0128] wherein, Y represents O or NH.

[0129] Examples of biotin or the derivative thereof can preferably include compounds represented by the following formulas (8) to (11).

##STR00022## ##STR00023##

[0130] Examples of biotin or the derivative thereof is more preferably the compound represented by the following formula (8).

##STR00024##

[0131] No text.

[0132] Where geometric isomers or optical isomers are present in the compounds of the present invention, mixtures or separations of those isomers are also included in the scope of the present invention. Separation of the isomers can be performed by a conventional method.

[0133] The target-affinity copolymer of the present invention can be produced by various known methods. The production method is not particularly limited, and for example, the target-affinity copolymer can be produced according to a synthesis method through the click reaction described below.

##STR00025##

[0134] The click chemistry is classified to reactions similar to natural biochemical reactions, having the following attributes, and has the following characteristics. The click chemistry is a very efficient reaction that proceeds rapidly to obtain high yields, and is a very selective reaction that produces no (or little) by-products and allows polyfunctional groups. Moreover, the click chemistry proceeds under mild reaction conditions such as a low temperature (or ambient temperature) or in an aqueous solution. This reaction can be performed in water or in a solvent such as an alcohol such as methanol, ethanol, 1-propanol, or 2-propanol; an ether such as diethyl ether, tetrahydrofuran, or 1,4-dioxane; an aromatic hydrocarbon such as benzene, toluene, or xylene; a halogenated hydrocarbon such as dichloromethane, chloroform, or 1,2-dichloroethane; N,N-dimethylformamide; N,N-dimethylacetamide; N-methylpyrrolidone; acetonitrile;

[0135] or ethyl acetate. It is preferable to use water or N,N-dimethylformamide as the solvent. In some aspects, the cyclooctyne is dibenzocyclooctyne (DBCO), difluorobenzocyclooctyne (DIFBO), biarylazacyclooctynone (BARAC), dibenzocyclooctyne (DIBO), difluorinated cyclooctyne (DIFO), monofluorinated cyclooctyne (MOFO), dimethoxyazacyclooctyne (DIMAC), or aryl-less octyne (ALO), with dibenzocyclooctyne (DBCO) being preferably used. In some aspects, the alkyne is aliphatic alkyne and the reacting step is performed in the presence of a copper (I) catalyst. In some aspects, the alkyne is cyclooctyne and the reacting step is performed under copper-free conditions. The reaction temperature is 0 to 300 C., preferably 0 to 150 C., and more preferably 1 to 100 C., and the reaction time is 1 minute to 48 hours, and preferably 5 minutes to 24 hours.

[0136] Reaction of the reaction formula is Huisgen cycloaddition which is a kind of click reaction, and is a 1,3-dipolar cycloaddition reaction in which 1,2,3-triazole is formed from the azide and the alkyne.

[0137] The produced polymer X and target-affinity copolymer of the present invention can be purified by a polymer isolation and purification method generally known in the field of polymer chemistry. Specific examples thereof include treatment operations such as extraction, recrystallization, salting out using, for example, ammonium sulfate or sodium sulfate, centrifugation, dialysis, ultrafiltration, adsorption chromatography, ion exchange chromatography, hydrophobic chromatography, normal phase chromatography, reverse phase chromatography, gel filtration, gel permeation chromatography, affinity chromatography, electrophoresis, countercurrent distribution, and combinations thereof.

[0138] The copolymer X and the target-affinity copolymer of the present invention can be used as a carrier for transporting of various physiologically active substances (drugs). For example, a pharmaceutical composition in which a therapeutic agent for tumor is carried on (encapsulated in) the copolymer X or the target-affinity copolymer of the present invention can be used as a prophylactic and/or therapeutic agent for various cancer diseases such as a colon cancer, a duodenal cancer, a gastric cancer, a pancreatic cancer, a liver cancer, a lung cancer, a uterine cancer, and an ovarian cancer, because the pharmaceutical composition suppresses growth of tumors, as confirmed in test examples described later. Further, with a high tumor accumulation ability, the pharmaceutical composition can be used as a diagnostic agent or a contrast agent for tumor.

[0139] When the copolymer and the target-affinity copolymer of the present invention are used as a drug transport carrier, a dose thereof and the number of administration times thereof may be appropriately selected in consideration of, for example, a dosage form, an age and body weight of a patient, and nature or severity of a symptom to be treated, and the dose and the number of administration times should not be limited. However, where the polymer containing the drug is intravenously injected by an injection, for example, for an adult (60 kg), a single dose is preferably administered in an amount of 0.12 mg to 12 000 000 mg, more preferably 1.2 mg to 1 200 000 mg, and particularly preferably 12 to 120 000 mg.

[0140] The pharmaceutical composition of the present invention can be produced by mixing the drug with the copolymer X or the target-affinity copolymer of the present invention. Preferably, the copolymer or the target-affinity copolymer of the present invention may be mixed with the drug to produce the single chain nanoparticle, or the single chain nanoparticle of the copolymer X or the target-affinity copolymer may be produced and then mixed with the drug. The single chain nanoparticle can be produced by a known method.

[0141] In the pharmaceutical composition of the present invention, the drug may be carried on the copolymer X or the target-affinity copolymer by the action such as electrostatic interaction, hydrogen bond, hydrophobic interaction, or covalent bond.

[0142] The drug may be any drug used for various cancer diseases, preferably an anticancer agent that acts on cancer cells to suppress proliferation of the cancer cells, and examples thereof include an antimetabolite, an alkylating agent, an anthracycline, an antibiotic, a mitotic inhibitor, a topoisomerase inhibitor, a proteasome inhibitor, and an anti-hormone agent.

[0143] Examples of the antimetabolite include azathioprine, 6-mercaptopurine, 6-thioguanine, fludarabine, pentostatin, cladribine, 5-fluorouracil (5FU), floxuridine (FUDR), cytosine arabinoside (cytarabine), methotrexate, trimethoprim, pyrimethamine, and pemetrexed. Examples of the alkylating agent include cyclophosphamide, mechlorethamine, uramustine, melphalan, chlorambucil, thiotepa/chlorambucil, ifosfamide, carmustine, lomustine, streptozocin, busulfan, dibromomannitol, cisplatin, carboplatin, nedaplatin, oxaliplatin, miriplatin, satraplatin, triplatin tetranitrate, procarbazine, altretamine, dacarbazine, mitozolomide, trabectedin, and temozolomide. Examples of the anthracycline include daunorubicin, doxorubicin, epirubicin, idarubicin, valrubicin, aclarubicin, amrubicin, and pirarubicin. Examples of the antibiotic include dactinomycin, bleomycin, mithramycin, anthramycin, streptozotocin, gramicidin D, staurosporine, mitomycins (for example, mitomycin C), duocarmycins (for example, CC-1065), and calicheamicins. Examples of the mitotic inhibitor include maytansinoids (for example, DM0, mertansine (also known as DM1), DM2, DM3, DM4, or emtansine), auristatins (for example, auristatin E, auristatin phenylalanine phenylenediamine (AFP), monomethyl auristatin E, monomethyl auristatin D, or monomethyl auristatin F), dolastatins, cryptophycins, vincal alkaloid (for example, vincristine, vinblastine, vindesine, or vinorelbine), taxanes (for example, paclitaxel or docetaxel), and colchicines. Examples of the topoisomerase inhibitor include irinotecan, topotecan, nogitecan, amsacrine, etoposide, teniposide, mizantrone, mitoxantrone, SN-38, exatecan, and deruxtecan. Examples of the proteasome inhibitor can include a peptidyl boronic acid, carfilzomib, and bortezomib. Examples of the anti-hormone agent can include fulvestrant, tamoxifen, and toremifene. Where these drugs are used to prepare the pharmaceutical composition of the present invention, one or more of the drugs can also be used in combination, and the drugs may be carried on the copolymer X or the target-affinity copolymer as a free form.

[0144] A route of administration of the pharmaceutical composition of the present invention is desirably the most effective one for treatment, and the pharmaceutical composition can be administered by a parenteral administration preparation such as an oral administration preparation, an injection, or a transdermal administration preparation. For example, parenteral administration such as intraarterial injection, intravenous injection, subcutaneous injection, intramuscular injection, or intraperitoneal injection is preferable, and intraarterial injection and intravenous injection are more preferable. The number of doses should not be limited, and examples thereof include one to several doses per week average.

[0145] Various preparations suitable for the route of administration can be produced by a conventional method by appropriately selecting preparation additives such as excipients, extenders, binders, wetting agents, disintegrants, lubricants, surfactants, dispersants, buffers, preservatives, solubilizing agents, antiseptics, flavoring agents, soothing agents, stabilizers, and isotonizing agents that are conventionally used in formulation.

[0146] The preparation additives that can be contained in the various preparations described above are not particularly limited as long as the preparation additives are pharmaceutically acceptable. Examples of such preparation additives include purified water, water for injection, distilled water for injection, pharmaceutically acceptable organic solvents, collagen, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, sodium alginate, water-soluble dextran, sodium carboxymethyl starch, pectin, xanthan gum, gum arabic, casein, gelatin, agar, glycerin, propylene glycol, polyethylene glycol, petrolatum, paraffin, stearyl alcohol, stearic acid, human serum albumin, mannitol, sorbitol, and lactose. Additives to be used are appropriately selected according to various preparations, and can be used alone or in combination.

[0147] The injection can also be prepared as a non-aqueous diluent (for example, polyethylene glycol, vegetable oils such as olive oil, and alcohols such as ethanol), a suspension, or an emulsion. Sterilization of the injection can be performed by filtration sterilization using a filter, and blending of, for example, a microbicide. In addition, the injection can be produced in a form of preparation before use. That is, the injection can be formed into a sterile solid composition by, for example, lyophilization, and can be dissolved in water for injection, distilled water for injection, or another solvent before use.

[0148] In a therapeutic method in which the target-affinity copolymer of the present invention is used, first, the fusion of the molecular probe such as the cancer antigen-specific antibody molecule and avidin/streptavidin or the mutant (modified) form or functional fragment thereof is prepared, and administered to the patient to accumulate the fusion on the cancer cell. Next, a process is performed to administer a conjugate of the target-affinity copolymer of the present invention and an anticancer agent to the patient, thereby accurately accumulating the drug on the cancer cell.

[0149] It is also possible to prepare a complex in which a conjugate of the molecular probe such as the cancer antigen-specific antibody molecule and the avidin/streptavidin or the mutant (modified) form or functional fragment thereof is bonded to a conjugate of the target-affinity copolymer of the present invention and the anticancer agent in advance and administer the complex to the patient.

EXAMPLES

[0150] Hereinafter, the present invention will be described more specifically with reference to examples. These examples are provided for purpose of exemplification and are not intended to limit embodiments of the invention.

[Example 1] Production of poly[(benzyl acrylate)-co-(poly(ethylene glycol)methyl ether acrylate)-co-(1-ethoxyethyl acrylate)]

(1) Synthesis of 1-ethoxyethyl acrylate (EEA)

[0151] Ethyl vinyl ether (28.725 mL) was weighed under an argon atmosphere, and thereto was added a phosphoric acid (50 mg) under ice cooling. Thereto was added acrylic acid (17.15 mL), and the mixture was stirred at room temperature for 48 hours. Further thereto was added hydrotalcite (3 g), and the mixture was stirred for 2 hours, and the reaction was stopped. After celite filtration, unreacted ethyl vinyl ether was removed by evaporation. Thereto was added phenothiazine (up to 500 ppm) as a polymerization inhibitor, and the mixture was purified by distillation under a reduced pressure together with calcium hydride (distillation temperature of 28 to 32 C.). The obtained 1-ethoxyethyl acrylate was dispensed into a glass vial and stored at 30 C.

[0152] .sup.13C NMR (400 MHZ, CDCl.sub.3), , ppm: 15.29 (OCH.sub.2CH.sub.3), 21.16 (COOCH(CH.sub.3)), 64.98 (OCH.sub.2), 96.73 (COOCH(CH.sub.3)), 128.84 (CH.sub.2CH), 131.43 (CH.sub.2CH), 166.00 (COO).

(2) Synthesis of poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(1-ethoxyethyl acrylate)]

[0153] 100 mg of 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAT) was weighed and dissolved in 17.3 mL of toluene to prepare a DDMAT/toluene stock solution (5.78 mg/mL as a DDMAT concentration). Similarly, 22 mg of 2,2-azobis(2-methylpropionitrile) (AIBN) was weighed and dissolved in 17.3 mL of toluene to prepare an AIBN/toluene stock solution (AIBN concentration: 1.27 mg/mL). Separately, 1.296 g of poly(ethylene glycol) methyl ether acrylate (mPEGA, the average value (n) of the numbers of repetitions of ethylene glycol is 9), 0.394 g of benzyl acrylate (BnA), 0.039 g of 1-ethoxyethyl acrylate, 1.73 mL of a DDMAT/toluene stock solution, and 1.73 mL of an AIBN/toluene stock solution were added, and polymerization was performed in an oil bath at 70 C. After a lapse of 90 minutes, the polymerization was stopped, and then the reaction solution was subjected to a reprecipitation method or dialyzed against methanol to recover the copolymer. Since the obtained copolymer was basically a viscous body, in the reprecipitation method, an operation of dropping the reaction solution into a centrifuge tube to which a poor solvent (hexane/ethyl acetate=7/3 [v/v]) was added and recovering the solution by centrifugation (2 000g, 5 min) was repeated 3 times, and finally vacuum drying was performed to obtain 1.223 g of poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(1-ethoxyethyl acrylate)].

[0154] As a result of analyzing the polymerization degree of each monomer and the number average molecular weight (M.sub.n,NMR) from a 1H-NMR spectrum of the obtained copolymer measured using NMR, the polymerization degree of mPEGA (n=9) was 102, the polymerization degree of BnA was 94, the polymerization degree of EEA was 9, and M.sub.n,NMR was 65 900. The molecular weight dispersion (M.sub.w/M.sub.n) of the obtained copolymer was measured using GPC, and as a result, it was found to be 1.53.

##STR00026##

[Measurement Apparatus and Conditions]

(1) .SUP.1.H-NMR Measurement

[0155] Apparatus: JNM-ECX 400 (400 MHz)/JEOL Ltd.

[0156] Solvent: Dimethyl sulfoxide-d.sub.6 containing 0.03% tetramethylsilane/KANTO CHEMICAL CO., INC.

[0157] Sample concentration: 20 mg/mL

[0158] Measurement temperature: 25 C.

[0159] Number of integration times: 256 times

[0160] Result: FIG. 1

(2) GPC Measurement

[0161] Apparatus: HPLC-Prominence system/SHIMADZU CORPORATION

[0162] Detector: RID-10A Refractive index detector/SHIMADZU CORPORATION

[0163] Column: TSKgel -2500 column/Tosoh Corporation

[0164] (Column size: 7.8 mm300 mm, particle size: 7 m, exclusion limit molecular weight: 510.sup.3)

[0165] TSKgel -4000 column/Tosoh Corporation

[0166] (Column size: 7.8 mm300 mm, particle size: 10 m, exclusion limit molecular weight: 410.sup.5)

[0167] TSKgel guard column/Tosoh Corporation

[0168] Mobile phase: N,N-dimethyformamide (DMF) containing 10 mmol/L lithium bromide

[0169] Temperature: 40 C.

[0170] Flow rate: 0.5 mL/min

[0171] Sample concentration: 6 mg/mL

[0172] Standard substance: Poly(methyl methacrylate) standard ReadyCal set, M.sub.p 800-2 200 000 Da/SIGMA

[0173] Result: FIG. 2

TABLE-US-00001 TABLE 1 Composition ratio (molar ratio to chain transfer agent) Chain Polymerization transfer Monomer degree agent Initiator mPEGA Temperature Polymerization Yield mPEGA M.sub.w/ Example DDMAT AlBN n = 9 BnA EEA Solvent ( C.) time (min) (g) n = 9 BnA EEA M.sub.n, NMR M.sub.n 1 1 0.5 100 90 10 Toluene 70 90 1.223 102 94 9 65 900 1.53

Examples 2 to 68

[0174] Polymers having different composition ratios and average molecular weights shown in the following table were produced by appropriately changing the type, charged amount, reaction temperature, and polymerization time of the monomers (mPEGA, BnA, and EEA) used in Example 1, and using the same method as in Example 1.

TABLE-US-00002 TABLE 2 Composition ratio (molar ratio to chain transfer agent) Monomer 2- Hydroxy- Chain 2- 4- 3- transfer Hydroxy Hydroxy phenoxy Temper- Polymer- agent Initiator mPEGA ethyl butyl propyl ature ization Example DDMAT AlBN ACHN n = 4 n = 9 n = 22 BnA EEA acrylate acrylate acrylate Solvent ( C.) time (min) 2 1 2 255 15 30 Toluene 70 90 3 1 2 160 20 20 Toluene 70 60 4 1 2 240 30 30 Toluene 70 60 5 1 2 170 10 20 Toluene 70 60 6 1 2 255 15 30 Toluene 70 60 7 1 0.5 16 16 8 Toluene 70 90 8 1 0.5 18 14 8 Toluene 70 90 9 1 0.5 20 12 8 Toluene 70 90 10 1 0.5 20 12 8 Toluene 70 90 11 1 0.5 20 12 8 Toluene 70 90 12 1 0.5 20 16 4 Toluene 70 10 13 1 0.5 20 16 4 Toluene 70 30 14 1 0.5 20 16 4 Toluene 70 50 15 1 0.5 20 16 4 Toluene 70 70 16 1 0.5 20 16 4 Toluene 70 90 17 1 0.5 22 10 8 Toluene 70 90 18 1 0.5 24 8 8 Toluene 70 90 19 1 0.5 24 8 8 Toluene 70 90 20 1 0.5 28 4 8 Toluene 70 90 21 1 0.5 10 25 15 Toluene 70 90 22 1 0.5 17 25 8 Toluene 70 90 23 1 0.5 22.5 12.5 15 Toluene 70 90 24 1 0.5 25 20 5 Toluene 70 90 25 1 0.5 29.5 12.5 8 Toluene 70 90 26 1 0.5 30 24 6 Toluene 70 90 27 1 0.5 35 28 7 Toluene 70 90 28 1 0.5 50 40 10 Toluene 70 90 29 1 0.5 10 10 180 Toluene 70 90 30 1 0.5 30 30 140 Toluene 70 90 31 1 0.5 30 70 100 Toluene 70 90 32 1 0.5 30 110 60 Toluene 70 90 33 1 0.5 50 10 140 Toluene 70 90 34 1 0.5 50 50 100 Toluene 70 90 35 1 0.5 50 90 60 Toluene 70 90 36 1 0.5 50 130 20 Toluene 70 90 37 1 0.5 70 30 100 Toluene 70 90 38 1 0.5 70 70 60 Toluene 70 90 39 1 0.5 70 110 20 Toluene 70 90 40 1 0.5 80 80 40 Toluene 70 90 41 1 0.5 80 100 20 Toluene 70 90 42 1 0.5 90 10 100 Toluene 70 90 43 1 0.5 90 50 60 Toluene 70 90 44 1 0.5 90 90 20 Toluene 70 90 45 1 0.5 100 20 80 Toluene 70 90 46 1 0.5 100 40 60 Toluene 70 90 47 1 0.5 100 50 50 Toluene 70 90 48 1 0.5 100 60 40 Toluene 70 90 49 1 0.5 100 70 30 Toluene 70 90 50 1 0.5 100 80 20 Toluene 70 90 51 1 0.5 110 30 60 Toluene 70 90 52 1 0.5 110 70 20 Toluene 70 90 53 1 0.5 130 10 60 Toluene 70 90 54 1 0.5 130 50 20 Toluene 70 90 55 1 0.5 150 30 20 Toluene 70 90 56 1 0.5 170 10 20 Toluene 70 90 57 1 0.5 200 160 40 Toluene 70 90 58 1 0.5 300 240 60 Toluene 70 90 59 1 0.5 400 320 80 Toluene 70 90 60 1 2 105 155 40 Toluene 70 90 61 1 2 120 240 40 Toluene 70 90 62 1 2 140 210 50 Toluene 70 90 63 1 2 140 220 40 Toluene 70 90 64 1 0.1 100 80 20 1,4- 70 90 dioxane 65 1 0.5 100 50 50 1,4- 70 180 dioxane 66 1 0.5 100 80 20 1,4- 70 180 dioxane 67 1 0.1 130 65 65 1,4 70 90 dioxane 68 1 0.1 130 104 26 1,4- 70 90 dioxane

TABLE-US-00003 TABLE 3 Polymerization degree 2- Hydroxy- 2-Hydroxy 4-Hydroxy 3-phenoxy mPEGA ethyl butyl propyl M.sub.w/ Example n = 4 n = 9 n = 22 BnA EEA acrylate acrylate acrylate M.sub.n NMR M.sub.n 2 250 18 31 62 400 1.37 3 155 22 20 39 200 1.23 4 211 29 29 53 100 1.32 5 162 11 20 39 000 1.22 6 227 15 28 54 300 1.33 7 16 16 8 11 800 1.20 8 18 14 8 12 400 1.20 9 13 8 5 8 600 1.46 10 20 13 8 13 400 1.30 11 18 11 7 11 700 1.33 12 8 7 1 5 300 1.51 13 16 13 3 10 800 1.30 14 19 15 3 12 200 1.28 15 20 16 4 13 300 1.39 16 24 19 4 15 700 1.29 17 21 10 8 13 200 1.20 18 13 5 4 8 000 1.44 19 24 8 8 14 300 1.21 20 26 4 8 14 600 1.20 21 7 17 9 8 100 1.41 22 11 17 5 9 200 1.39 23 15 9 10 10 700 1.30 24 26 21 5 16 900 1.31 25 20 10 6 12 400 1.33 26 33 27 6 21 500 1.34 27 39 30 7 25 000 1.36 28 39 4 8 20 200 1.25 29 10 10 159 29 700 1.19 30 27 27 122 35 600 1.27 31 30 66 91 38 600 1.17 32 27 91 52 35 800 1.20 33 45 10 123 41 000 1.31 34 44 45 86 41 100 1.20 35 45 79 54 42 700 1.24 36 43 111 19 42 000 1.28 37 60 28 87 46 500 1.24 38 55 57 49 43 000 1.27 39 56 89 18 44 300 1.30 40 79 80 36 56 400 1.43 41 81 106 17 58 900 1.51 42 81 12 93 54 600 1.27 43 71 42 51 48 600 1.30 44 70 73 18 48 400 1.32 45 84 19 72 54 200 1.33 46 80 35 52 51 900 1.35 47 84 45 44 54 200 1.33 48 82 52 35 53 100 1.36 49 94 68 26 60 100 1.50 50 104 83 20 66 700 1.52 51 93 30 55 57 700 1.33 52 84 56 18 52 400 1.35 53 105 10 52 60 000 1.35 54 102 42 18 58 900 1.37 55 111 25 18 60 100 1.40 56 131 10 18 67 400 1.43 57 129 108 31 84 100 1.51 58 170 144 40 111 200 1.61 59 221 191 50 144 700 1.68 60 58 102 78 91 200 1.71 61 62 143 21 93 100 1.68 62 59 105 93 95 000 1.82 63 60 106 18 85 400 1.68 64 75 66 19 49 400 1.46 65 67 37 39 44 300 1.40 66 61 56 20 41 600 1.43 67 75 42 42 52 300 1.61 68 75 68 18 51 400 1.56

Example 69

Production of poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)]

[0175] By treating poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(1-ethoxyethyl acrylate)] obtained in Example 1 with 0.5N HCl at room temperature, an ethoxyethyl group was eliminated to obtain a terpolymer having a carboxyl group (1.176 g). A Z-average particle diameter and a polydispersity index of the obtained terpolymer in water were measured by dynamic light scattering (DLS), and as a result, the Z-average particle diameter was 8.5 nm (polydispersity index: 0.14).

##STR00027##

[Measurement Apparatuses and Conditions]

(1) DLS Measurement

[0176] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0177] Measurement temperature: 25 C.

[0178] Sample concentration: 10 mg/mL

[0179] Results: FIG. 3

Example 70

Method for Producing (1,2-diaminocyclohexane)platinum (II)-Encapsulating SCNP

[0180] 65.28 mg of a Cl(H.sub.2O) form of (1,2-diaminocyclohexane) platinum (II) (hereinafter, abbreviated as DACHPt) (DACHPt.Math.Cl.Math.H.sub.2O) was dissolved in 20 mL of purified water and stirred at 70 C. for 2 hours. To 5 mL of this solution, 287.4 mg of the terpolymer obtained in Example 69 was added, and the mixture was stirred at 50 C. overnight. After completion of stirring, the reaction solution was dialyzed and purified using purified water as an external solution to obtain 5 mL of a DACHPt-encapsulating SCNP. The Pt content of the DACHPt-encapsulating SCNP obtained after purification was measured by inductively coupled plasma-atomic emission spectrometry (ICP-AES) and found to be 720 g/mL (1.14 mg/mL as DACHPt). Separately, 200 L of the DACHPt-encapsulating SCNP was lyophilized and the solid content concentration was calculated, then the ratio to the Pt content was taken, and the Pt binding amount per polymer was calculated. As a result, the Pt binding amount was 3.4 mol/mol. In addition, the Z-average particle diameter and the polydispersity index of the obtained DACHPt-encapsulating SCNP were measured by dynamic light scattering (DLS), and as a result, the Z-average particle diameter was found to be 8.7 nm (polydispersity index: 0.14). The particle diameter of the SCNP before and after encapsulation of DACHPt is shown in FIG. 3. The particle diameter of the SCNP hardly varied before and after encapsulation of DACHPt. The results are summarized in the following table.

[Measurement Apparatuses and Conditions]

(1) ICP-AES Measurement

[0181] Apparatus: Sequential high-frequency plasma light-emitting apparatus ICPE-9000/SHIMADZU CORPORATION

[0182] Pretreatment apparatus: microwave sample pretreatment apparatus ETHOS EASY/Milestone General

[0183] Measurement wavelength: 214 nm

[0184] Standard solution: Platinum standard solution (Pt1000) for ICP analysis/FUJIFILM Wako Pure Chemical Corporation

2) DLS Measurement

[0185] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0186] Measurement temperature: 25 C.

[0187] Sample concentration: 10 mg/mL

[0188] Results: FIG. 3

TABLE-US-00004 TABLE 4 Z-average Pt content particle Yield Pt content per polymer diameter Polydispersity Example (mL) (g/mL) (mol/mol) (nm) index 70 5 720 3.4 8.7 0.14

Example 71

Production of N.SUB.3.-poly[(benzyl acrylate)-co-(poly(ethylene glycol)methyl ether acrylate)-co-(1-ethoxyethyl acrylate)]

[0189] Synthesis was performed by using N.sub.3-CTA in place of the chain transfer agent DDMAT used in Example 1. In toluene (17.3 mL), 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid 3-azido-1-propanol ester (N.sub.3-CTA) (100 mg) was dissolved after being weighed to obtain an N.sub.3-CTA/toluene stock solution (N.sub.3-CTA concentration: 5.78 mg/mL). Similarly, 2,2-azobis(2-methylpropionitrile) (AIBN) (10 mg) was weighed and dissolved in toluene (7.87 mL) to obtain an AIBN/toluene stock solution (AIBN concentration: 1.27 mg/mL). Separately, poly(ethylene glycol) methyl ether acrylate (mPEGA, average value (n) of the numbers of repetition times of ethylene glycol is 9) (2.592 g), benzyl acrylate (BnA) (0.684 g), 1-ethoxyethyl acrylate (0.172 g), the N.sub.3-CTA/toluene stock solution (4.15 mL), and the AIBN/toluene stock solution (3.46 mL) were added, and polymerization was performed in an oil bath at 70 C. After a lapse of 90 minutes, the polymerization was stopped, and then a copolymer was recovered by subjecting the reaction solution to reprecipitation or dialysis against methanol. Since the obtained copolymer was basically a viscous form, for the reprecipitation, an operation of dropping the reaction solution into a centrifuge tube to which the poor solvent (hexane/ethyl acetate=7/3 [v/v]) was added and recovering the solution by centrifugation (2 000g, 5 min) was repeated three times, and finally the vacuum drying was performed to obtain N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(1-ethoxyethyl acrylate)] (2.643 g).

[0190] As a result of analyzing a polymerization degree of each of monomers and a number average molecular weight (M.sub.n,NMR) Of the obtained copolymer from a .sup.1H-NMR spectrum measured by using NMR, the polymerization degree of mPEGA (n=9) was 70, the polymerization degree of BnA was 56, the polymerization degree of EEA was 15, and M.sub.n,NMR was 44 000. Moreover, a molecular weight dispersion (M.sub.w/M.sub.n) Of the obtained copolymer was measured by using the GPC and a result thereof was 1.32.

Example 72

Production of N.SUB.3.-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)]

[0191] By treating N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(1-ethoxyethyl acrylate)] obtained in Example 71 with 0.5N HCl at room temperature, an ethoxyethyl group was eliminated to obtain a terpolymer having a carboxyl group (2.455 g). A Z-average particle diameter and a polydispersity index of the obtained terpolymer in water were measured by dynamic light scattering (DLS), and a result of the Z-average particle diameter was 7.5 nm (polydispersity index: 0.18).

Example 73

Production of Biotin-Bonded SCNP

[0192] To DMF (5 mL) in a glass vial, were added N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)] (275 mg) and ((1R,8S,9S)-bicyclo[6.1.0]non-4-yn-9-yl)methyl (22-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-9-(2-((4-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)butyl)amino)-2-oxoethyl)-11,18-dioxo-3,6-dioxa-9,12,17-triazadocosyl) carbamate (19.5 mg) and the mixture was stirred overnight at room temperature. The reaction solution was dialyzed against methanol, the solvent was distilled off, and then the biotin-bonded SCNP (265.7 mg) was recovered by the vacuum drying. A Z-average particle diameter and a polydispersity index of the biotin-bonded SCNP were measured by dynamic light scattering (DLS), and a result of the Z-average particle diameter was 8.6 nm (polydispersity index: 0.17).

##STR00028##

[Measurement Apparatuses and Conditions]

(1) .SUP.1.H-NMR Measurement

[0193] Apparatus: JNM-ECX400 (400 MHz)/JEOL Ltd.

[0194] Solvent: dimethyl sulfoxide-d.sub.6 containing 0.03% tetramethylsilane/KANTO CHEMICAL CO., INC.

[0195] Sample concentration: 20 mg/mL

[0196] Measurement temperature: 25 C.

[0197] Number of integration times: 6 000 times

[0198] Results: FIG. 4

(2) GPC Measurement

[0199] Apparatus: HPLC-Prominence system/SHIMADZU CORPORATION

[0200] Detector: RID-10A Refractive index detector/SHIMADZU CORPORATION

[0201] Column: TSKgel -2500 column/Tosoh Corporation

[0202] (Column size: 7.8 mm300 mm, particle size: 7 m, exclusion limit molecular weight: 510.sup.3)

[0203] TSKgel -4000 column/Tosoh Corporation

[0204] (Column size: 7.8 mm300 mm, particle size: 10 m, exclusion limit molecular weight: 410.sup.5)

[0205] TSKgel guard column/Tosoh Corporation

[0206] Mobile phase: N,N-dimethyformamide (DMF) containing 10 mmol/L lithium bromide

[0207] Temperature: 40 C.

[0208] Flow rate: 0.5 mL/min

[0209] Sample concentration: 6 mg/mL

[0210] Standard substance: Poly(methyl methacrylate) standard ReadyCal set, M.sub.p 800-2 200 000 Da/SIGMA

[0211] Result: FIG. 5

(3) DLS Measurement

[0212] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0213] Measurement temperature: 25 C.

[0214] Sample concentration: 10 mg/mL

[0215] Results: FIG. 6

Example 74

Synthesis of ((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methyl(2-(2-(2-(3,5-bis(6-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)benzamido)ethoxy)ethoxy)ethyl)carbamate di(2,2,2-trifluoroacetate)

[0216] To (3aS,3aS,4S,4S,6aR,6aR)-4,4-{5,5-[6,6-(5-(2-(2-(2-aminoethoxy)ethoxy)ethylcarbamoyl)-1,3-phenylene)bis(azanediyl)bis(6-oxohexane-6,1-diyl)]bis(azanediyl)bis(5-oxopentane-5,1-diyl)}bis(tetrahydro-1H-thieno[3,4-d]imidazol-2(3H)-iminium)tri(2,2,2-trifluoroacetate) (415 mg), DMF (15 mL), triethylamine (445 L), and (1R,8S,9S)-bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN-NHS) (93.0 mg) were added, and the mixture was stirred at room temperature for 2 hours. After concentration, purification was performed by reverse phase silica gel column chromatography (0.1% TFA aqueous solution/MeOH=90/10 to 30/70) to obtain ((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methyl(2-(2-(2-(3,5-bis(6-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)benzamido)ethoxy)ethoxy)ethyl)carbamate di(2,2,2-trifluoroacetate) (compound (9)) (302 mg).

##STR00029##

[0217] .sup.1H

[0218] NMR (400 MHz, CD.sub.3OD) :8.02 (brs, 1H), 7.72 (d, J=1.6 Hz, 2H), 4.71 (dd, J=4.4, 7.6 Hz, 2H), 4.52 (dd, J=4.4, 7.6 Hz, 2H), 4.09 (d, J=8.4 Hz, 2H), 3.70-3.62 (m, 6H), 3.59-3.52 (m, 4H), 3.29-3.24 (m, 4H), 3.19 (t, J=7.2 Hz, 4H), 2.98 (dd, J=4.8, 13.2 Hz, 2H), 2.81 (d, J=13.2 Hz, 2H), 2.40 (t, J=7.2 Hz, 4H), 2.26-2.10 (m, 10H), 1.79-1.51 (m, 18H), 1.47-1.38 (m, 8H), 1.38-1.30 (m, 1H), 0.90 (t, J=9.6 Hz, 2H)

Example 75

Production of Compound (9)-Bonded SCNP

[0219] In a glass vial, N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)] (200 mg) and the compound (9) obtained in Example 74 (30 mg) were dissolved in DMF (5 mL), and the mixture was stirred at room temperature overnight. The reaction solution was dialyzed against methanol, the solvent was distilled off, and then the compound (9)-bonded SCNP (197.4 mg) was recovered by the vacuum drying. A Z-average particle diameter and a polydispersity index of the compound (9)-bonded SCNP were measured by dynamic light scattering (DLS), and a result of the Z-average particle diameter was 7.2 nm (polydispersity index: 0.18).

[Measurement Apparatuses and Conditions]

(1) .SUP.1.H-NMR Measurement

[0220] Apparatus: JNM-ECX400 (400 MHz)/JEOL Ltd.

[0221] Solvent: dimethyl sulfoxide-d.sub.6 containing 0.03% tetramethylsilane/KANTO CHEMICAL CO., INC.

[0222] Sample concentration: 20 mg/mL

[0223] Measurement temperature: 25 C.

[0224] Number of integration times: 6 000 times

[0225] Results: FIG. 7

(2) GPC Measurement

[0226] Apparatus: HPLC-Prominence system/SHIMADZU CORPORATION

[0227] Detector: RID-10A Refractive index detector/SHIMADZU CORPORATION

[0228] Column: TSKgel -2500 column/Tosoh Corporation

[0229] (Column size: 7.8 mm300 mm, particle size: 7 m, and exclusion limit molecular weight: 510.sup.3)

[0230] TSKgel -4000 column/Tosoh Corporation

[0231] (Column size: 7.8 mm300 mm, particle size: 10 m, and exclusion limit molecular weight: 410.sup.5)

[0232] TSKgel guard column/Tosoh Corporation

[0233] Mobile phase: N,N-dimethyformamide (DMF) containing 10 mmol/L of lithium bromide

[0234] Temperature: 40 C.

[0235] Flow rate: 0.5 mL/min

[0236] Sample concentration: 6 mg/mL

[0237] Standard substance: poly(methyl methacrylate) standard ReadyCal set, M.sub.p 800-2 200 000 Da/SIGMA

[0238] Results: FIG. 8

(3) DLS Measurement

[0239] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0240] Measurement temperature: 25 C.

[0241] Sample concentration: 10 mg/mL

[0242] Results: FIG. 9

Example 76

Synthesis of ((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methyl(2-(2-(2-(3-(3,5-bis((2-(2-(5-((3as,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethyl)carbamoyl)phenyl)ureido)ethoxy)ethoxy)ethyl)carbamate di(2, 2,2-trifluoroacetate)

[0243] To 5-(3-(2-(2-(2-aminoethoxy) ethoxy)ethyl) ureido)-N.sup.1,N.sup.3-bis(2-(2-(5-((3aS,3aS,4S,4S,6aR,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide)ethoxy)ethyl)isophthalamide tri(2,2,2-trifluoroacetate) (450 mg), DMF (15 mL), Et.sub.3N (475 L), and BCN-NHS (99.3 mg) were added, and the mixture was stirred at room temperature for 2 hours. After concentration, purification was performed by reverse phase silica gel column chromatography (0.1% TFA aqueous solution/MeOH=90/10 to 30/70) to obtain ((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)methyl(2-(2-(2-(3-(3,5-bis((2-(2-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethyl)carbamoyl)phenyl)ureido)ethoxy) ethoxy)ethyl)carbamate di(2, 2,2-trifluoroacetate) (compound (10)) (327 mg).

##STR00030##

[0244] .sup.1H

[0245] NMR (400 MHz, CD.sub.3OD) :8.00 (d, J=1.2 Hz, 2H), 7.88 (t, J=1.6 Hz, 1H), 4.71 (dd, J=4.4, 7.6 Hz, 2H), 4.50 (dd, J=4.0, 7.6 Hz, 2H), 4.17 (d, J=8.4H z, 2H), 3.67-3.63 (m, 8H), 3.62-3.51 (m, 12H), 3.42 (t, J=5.2 Hz, 2H), 3.38 (t, J=5.2 Hz, 4H), 3.29-3.27 (m, 2H), 3.26-3.19 (m, 2H), 2.98 (dd, J=4.8, 12.8 Hz, 2H), 2.81 (d, J=12.8 Hz, 2H), 2.26-2.10 (m, 10H), 1.73-1.62 (m, 2H), 1.61-1.46 (m, 8H), 1.41-1.28 (m, 5H), 0.90 (t, J=9.6 Hz, 2H)

Example 77

Production of Compound (10)-Bonded SCNP

[0246] In a glass vial, N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)] (200 mg) and the compound (10) obtained in Example 76 (30 mg) were dissolved in DMF (5 mL), and the mixture was stirred at room temperature overnight. The reaction solution was dialyzed against methanol, the solvent was distilled off, and then the compound (10)-bonded SCNP (195.8 mg) was recovered by the vacuum drying. A Z-average particle diameter and a polydispersity index of the compound (10)-bonded SCNP were measured by dynamic light scattering (DLS), and a result of the Z-average particle diameter was 8.3 nm (polydispersity index: 0.19).

[Measurement Apparatuses and Conditions]

(1) .SUP.1.H-NMR Measurement

[0247] Apparatus: JNM-ECX400 (400 MHz)/JEOL Ltd.

[0248] Solvent: dimethyl sulfoxide-d.sub.6 containing 0.03% tetramethylsilane/KANTO CHEMICAL CO., INC.

[0249] Sample concentration: 20 mg/mL

[0250] Measurement temperature: 25 C.

[0251] Number of integration times: 6 000 times

[0252] Results: FIG. 10

(2) GPC Measurement

[0253] Apparatus: HPLC-Prominence system/SHIMADZU CORPORATION

[0254] Detector: RID-10A Refractive index detector/SHIMADZU CORPORATION

[0255] Column: TSKgel -2500 column/Tosoh Corporation

[0256] (Column size: 7.8 mm300 mm, particle size: 7 m, and exclusion limit molecular weight: 510.sup.3)

[0257] TSKgel -4000 column/Tosoh Corporation

[0258] (Column size: 7.8 mm300 mm, particle size: 10 m, and exclusion limit molecular weight: 410.sup.5)

[0259] TSKgel guard column/Tosoh Corporation

[0260] Mobile phase: N,N-dimethy formamide (DMF) containing 10 mmol/L of lithium bromide

[0261] Temperature: 40 C.

[0262] Flow rate: 0.5 mL/min

[0263] Sample concentration: 6 mg/mL

[0264] Standard substance: poly(methyl methacrylate) standard ReadyCal set, M.sub.p 800 to 2 200 000 Da/SIGMA

[0265] Results: FIG. 11

(3) DLS Measurement

[0266] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0267] Measurement temperature: 25 C.

[0268] Sample concentration: 10 mg/mL

[0269] Results: FIG. 12

Example 78

Synthesis of dimethyl 6,6-((5-(3-(1-((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)-3-oxo-2,7,10-trioxa-4-azadodecane-12-yl)ureido)isophthaloyl)bis(azanediyl)) (2S,2S)-bis(2-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentamido)hexanoate) di(2,2,2-trifluoroacetate)

[0270] To (R,S,S,2S,2S)-dimethyl 6,6-((5-(3-(2-(2-(2-aminoethoxy)ethoxy)ethyl)ureido)isophthaloyl)bis(azanediyl))bis(2-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide)hexanoate) tri(2,2,2-trifluoroacetate) (165 mg), DMF (10 mL), Et.sub.3N (160 L), and BCN-NHS (33.6 mg) were added, and the mixture was stirred at room temperature for 2 hours. After concentration, purification was performed by reverse phase silica gel column chromatography (0.1% TFA aqueous solution/MeOH=90/10 to 30/70) to obtain dimethyl 6,6-((5-(3-(1-((1R,8S,9s)-bicyclo[6.1.0]non-4-yn-9-yl)-3-oxo-2,7,10-trioxa-4-azadodecane-12-yl)ureido)isophthaloyl)bis(azanediyl)) (2S,2S)-bis(2-(5-((3aS,4S,6aR)-2-iminohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentamido)hexanoate) di(2, 2,2-trifluoroacetate) (compound (11)) (120 mg).

##STR00031##

[0271] .sup.1H

[0272] NMR (400 MHz, CD.sub.3OD) :7.95 (d, J=1.6 Hz, 2H), 7.81 (t, J=1.6 Hz, 1H), 4.72 (dd, J=4.4, 7.6 Hz, 2H), 4.52 (dd, J=4.4, 7.6 Hz, 2H), 4.41 (dd, J=4.8, 9.6 Hz, 2H), 4.11 (d, J=7.6 Hz, 2H), 3.71 (s, 6H), 3.65 (s, 4H), 3.60 (t, J=5.6 Hz, 2H), 3.55 (t, J=5.6 Hz, 2H), 3.44-3.37 (m, 6H), 3.29-3.25 (m, 4H), 2.99 (dd, J=4.8, 12.8 Hz, 2H), 2.81 (d, J=13.2 Hz, 2H), 2.28-2.10 (m, 10H), 1.94-1.84 (m, 2H), 1.79-1.32 (m, 25H), 0.90 (t, J=9.6 Hz, 2H)

Example 79

Production of Compound (11)-Bonded SCNP

[0273] In a glass vial, N.sub.3-poly[(benzyl acrylate)-co-(poly(ethylene glycol) methyl ether acrylate)-co-(acrylic acid)] (200 mg) and the compound (11) obtained in Example 78 (30 mg) were dissolved in DMF (5 mL), and the mixture was stirred at room temperature overnight. The reaction solution was dialyzed against methanol, the solvent was distilled off, and then the compound (11)-bonded SCNP (198.5 mg) was recovered by the vacuum drying. A Z-average particle diameter and a polydispersity index of the compound (11)-bonded SCNP were measured by dynamic light scattering (DLS), and a result of the Z-average particle diameter was 8.6 nm (polydispersity index: 0.25).

[Measurement Apparatuses and Conditions]

(1) .SUP.1.H-NMR Measurement

[0274] Apparatus: JNM-ECX400 (400 MHz)/JEOL Ltd.

[0275] Solvent: dimethyl sulfoxide-de containing 0.03% tetramethylsilane/KANTO CHEMICAL CO., INC.

[0276] Sample concentration: 20 mg/mL

[0277] Measurement temperature: 25 C.

[0278] Number of integration times: 6 000 times

[0279] Results: FIG. 13

(2) GPC Measurement

[0280] Apparatus: HPLC-Prominence system/SHIMADZU CORPORATION

[0281] Detector: RID-10A Refractive index detector/SHIMADZU CORPORATION

[0282] Column: TSKgel -2500 column/Tosoh Corporation

[0283] (Column size: 7.8 mm300 mm, particle size: 7 m, and exclusion limit molecular weight: 510.sup.3)

[0284] TSKgel -4000 column/Tosoh Corporation

[0285] (Column size: 7.8 mm300 mm, particle size: 10 m, and exclusion limit molecular weight: 410.sup.5)

[0286] TSKgel guard column/Tosoh Corporation

[0287] Mobile phase: N,N-dimethyformamide (DMF) containing 10 mmol/L of lithium bromide

[0288] Temperature: 40 C.

[0289] Flow rate: 0.5 mL/min

[0290] Sample concentration: 6 mg/mL

[0291] Standard substance: poly(methyl methacrylate) standard ReadyCal set, M.sub.p 800-2 200 000 Da/SIGMA

[0292] Results: FIG. 14

(3) DLS Measurement

[0293] Apparatus: Zetasizer NanoZS/Malvern Instruments Ltd.

[0294] Measurement temperature: 25 C.

[0295] Sample concentration: 10 mg/mL

[0296] Results: FIG. 15

Comparative Example 1

Preparation of Oxaliplatin Solution

[0297] To 5.58 mL of a 5.9 (w/v) % glucose solution was added 1 mL of ELPLAT I.V. infusion solution 50 mg (Yakult Honsha Co., Ltd.) to prepare a 5 (w/v) % glucose solution containing 760 g of oxaliplatin.

[Test Example 1] Drug Efficacy Test

[0298] A tumor-bearing model obtained by subcutaneously transplanting a mouse colon cancer cell line C26 (American Type Culture Collection) into a female nude mouse (BALB/c-nu nu/nu, 7 weeks old; Charles River Laboratories Japan, Inc.) was used for a drug efficacy test.

[0299] A mouse colon cancer cell line C26 subcultured in a CO.sub.2 incubator was suspended in a liquid medium (Dulbecco's Modified Eagle's Medium-high glucose, Sigma-Aldrich Co. LLC), and injected subcutaneously in the back of nude mice so that the number of cells per mouse was 110.sup.6/100 L. Thereafter, the nude mice were raised for about 1 week, and administration of a drug was started when the average value of the tumor volume was grown to about 30 mm.sup.3. A DACHPt-encapsulating SCNP (DACHPt-encapsulating SCNP prepared using the copolymer of Example 70) was administered into the tail vein (3 times every other day), and the antitumor effect was evaluated from the tumor volume (4 to 5 mice in 1 group). As a comparison, an oxaliplatin solution (Comparative Example 1) was used, and the oxaliplatin solution was administered in the same manner. The dose of each preparation was 8 mg/kg (3.9 mg/kg in terms of Pt) as the maximum administrable dose for the oxaliplatin solution, and 3 mg/kg in terms of Pt for the DACHPt-encapsulating SCNP.

[0300] A change in the tumor volume over time is shown in FIG. 16. For the DACHPt-encapsulating SCNP, T/C was 0.4 after 14 days from administration [T/C: ratio of the tumor volume of the drug administration group (T) to that of the control group (C)]. For the oxaliplatin solution (Comparative Example 1), T/C was 1.1 after 14 days from administration. In addition, after 14 days from administration, it was confirmed that the DACHPt-encapsulating SCNP significantly suppressed growth of a tumor as compared with the control (student's t-test). The above results indicate that the DACHPt-encapsulating SCNP has an excellent antitumor effect as compared with the oxaliplatin solution.