METHODS OF ADMINISTERING NATURAL KILLER CELLS COMPRISING AN ANTI-HUMAN EPIDERMAL GROWTH FACTOR RECEPTOR 2 (HER2) CHIMERIC ANTIGEN RECEPTOR (CAR)

Abstract

Provided herein, among other things, are methods of administering NK cells comprising poly nucleotides comprising a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR).

Claims

1. A method comprising administering from 1 million to 100 billion natural killer (NK) cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15.

2. The method of claim 1, wherein the NK cells are administered in a dose comprising from 1 billion to 100 billion NK cells, optionally in a dose comprising at or about 1 million to at or about 20 billion cells (e.g., at or about 5 million cells, at or about 25 million cells, at or about 50 million cells, at or about 75 million cells, at or about 100 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, or a range defined by any two of the foregoing values), at or about 10 million to at or about 20 billion cells (e.g., at or about 25 million cells, at or about 50 million cells, at or about 75 million cells, at or about 100 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, at or about 20 billion cells, or a range defined by any two of the foregoing values), and in some cases at or about 100 million cells to at or about 50 billion cells (e.g., at or about 150 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, at or about 20 billion cells, at or about 30 billion cells, at or about 40 billion cells).

3. The method of claim 1, where the NK cells are administered in a dose comprising at least 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 cells per dose, at or at least 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 510.sup.7, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 cells per dose, at or at least 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 cells per dose, at least 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 cells per dose, or at least 110.sup.10 or 210.sup.10 cells per dose.

4. The method of claim 1, where the NK cells are administered in a dose comprising about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 cells per dose, about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 510.sup.7, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 cells per dose, about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 cells per dose, about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 cells per dose, or about 110.sup.10 or 210.sup.10 cells per dose.

5. The method of claim 1, wherein the NK cells are administered in a dose comprising at or about 1 million to at or about 20 billion CAR-expressing NK cells (e.g., at or about 5 million cells, at or about 25 million CAR-expressing cells, at or about 50 million CAR-expressing cells, at or about 75 million CAR-expressing cells, at or about 100 million CAR-expressing cells, at or about 200 million CAR-expressing cells, at or about 300 million CAR-expressing cells, at or about 400 million CAR-expressing cells, at or about 500 million CAR-expressing cells, at or about 1 billion CAR-expressing cells, at or about 2 billion CAR-expressing cells, at or about 3 billion CAR-expressing cells, at or about 4 billion CAR-expressing cells, at or about 5 billion CAR-expressing cells, at or about 6 billion CAR-expressing cells, at or about 7 billion CAR-expressing cells, at or about 8 billion CAR-expressing cells, at or about 9 billion CAR-expressing cells, at or about 10 billion CAR-expressing cells, or at or about 20 billion CAR-expressing cells, or a range defined by any two of the foregoing values) per dose.

6. The method of claim 1, wherein the NK cells are administered in a dose comprising at or at about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 510.sup.7, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 CAR-expressing cells per dose, or a dose comprising at or at about 110.sup.10 or at or at about 210.sup.10 CAR-expressing cells per dose.

7. The method of claim 1, wherein the NK cells are administered in a dose comprising at least or at least about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 CAR-expressing cells per dose, at least or at least about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 5107, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 CAR-expressing cells per dose, at least or at least about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 CAR-expressing cells per dose, at least or at least about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 CAR-expressing cells per dose, at least or at least about 110.sup.10 CAR-expressing cells per dose.

8. A method comprising administering NK cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15, wherein the NK cells are administered in a dose comprising 110.sup.5 to 1010.sup.8 cells/kg.

9. The method of claim 8, wherein the dose of cells comprises between at or about 110.sup.5 of the cells/kg and at or about 110.sup.8 of the cells/kg, such as between at or about 1.510.sup.5 of the cells/kg and at or about 1.510.sup.7 of the cells/kg, or between at or about 410.sup.5 of the cells/kg and at or about 410.sup.6 of the cells/kg.

10. The method of claim 8, wherein the NK cells are administered in a dose comprising at or at about 110.sup.5, 1.510.sup.5, 210.sup.5, 2.510.sup.5, 310.sup.5, 3.510.sup.5, 410.sup.5, 4.510.sup.5, 510.sup.5, 5.510.sup.5, 610.sup.5, 6.510.sup.5, 710.sup.5, 7.510.sup.5. 810.sup.5, 8.510.sup.5, 910.sup.5, or 9.510.sup.5 cell/kg, 110.sup.6, 1.510.sup.6, 210.sup.6, 2.510.sup.6, 310.sup.6, 3.510.sup.6, 410.sup.6, 4.510.sup.6, 510.sup.6, 5.510.sup.6, 610.sup.6, 6.510.sup.6, 710.sup.6, 7.510.sup.6, 810.sup.6, 8.510.sup.6, 910.sup.6, or 9.510.sup.6 cells/kg, at or at about 110.sup.7, 1.510.sup.7, 210.sup.7, 2.510.sup.7, 310.sup.7, 3.510.sup.7, 410.sup.7, 4.510.sup.7, 510.sup.7, 5.510.sup.7, 610.sup.7, 6.510.sup.7, 710.sup.7, 7.510.sup.7, 810.sup.7, 8.510.sup.7, 910.sup.7 cells/kg, or 9.510.sup.7, or at or at about 110.sup.8, 1.510.sup.8, or 210.sup.8 cells/kg.

11. The method of claim 8, wherein the NK cells are administered in a dose comprising at least or at least about 110.sup.5, 1.510.sup.5, 210.sup.5, 2.510.sup.5. 310.sup.5, 3.510.sup.5, 410.sup.5, 4.510.sup.5, 510.sup.5, 5.510.sup.5, 610.sup.5, 6.510.sup.5, 710.sup.5, 7.510.sup.5, 810.sup.5, 8.510.sup.5, 910.sup.5, or 9.510.sup.5 cell/kg, at least or at least about 110.sup.6, 1.510.sup.6, 210.sup.6, 2.510.sup.6, 310.sup.6, 3.510.sup.6, 410.sup.64.510.sup.6, 510.sup.6, 5.510.sup.6, 610.sup.6, 6.510.sup.6, 710.sup.6, 7.510.sup.6, 810.sup.6, 8.510.sup.6, 910.sup.6, or 9.510.sup.6 cells/kg, at least or at least about 110.sup.7, 1.510.sup.7, 210.sup.7, 2.510.sup.7, 310.sup.7, 3.5107, 410.sup.7, 4.510.sup.7, 510.sup.7, 5.510.sup.7, 610.sup.7, 6.510.sup.7, 710.sup.7, 7.510.sup.7, 810.sup.7, 8.510.sup.7, 910.sup.7 cells/kg, or 9.510.sup.7, or at least or at least about 110.sup.8 or 1.510.sup.8 cells/kg.

12. A method comprising administering multiple doses of NK cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15, and wherein the doses are administered once every one to sixteen weeks.

13. The method of claim 12, wherein the NK cells are administered bi-weekly, once every three weeks, once every four weeks, once every five weeks, once every six weeks, once every seven weeks, once every eight weeks, once every nine weeks, once every ten weeks, once every 11 weeks, once every 12 weeks, once every 13 weeks, once every 14 weeks, once every 15 weeks, or once every 16 weeks.

14. A method comprising administering NK cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15, wherein the patient meets one or more of the following criteria: (i) the patient has an Eastern Cooperative Oncology Group (ECOG) performance status of from 0 to 4 at the time of treatment: (ii) the patient is refractory to or has a recurrence after receiving 1 or more prior systemic therapies: (iii) the patient has an oxygen saturation level measured via pulse oximeter of at least 92%: (iv) the patient has a left ventricular ejection fraction (LVEF) of at least 50%; (v) the patient has an absolute neutrophil count (ANC) of at least 1000/mm.sup.3 (1.0109/L); (vi) the patient has a platelet count of at least 75000/mm.sup.3 (7510.sup.9 /L); (vii) the patient's hemoglobin level, with or without prior transfusion, is at least 8.0 g/dL: (viii) the patient's creatinine clearance is at least 45 mL/min using the Cockcraft-Gault equation, or mL/min/1.73 m.sup.2 as estimated glomerular filtration rate (eGFR) per Modification of Diet in Renal Disease (MDRD) equation: (ix) the patient's total serum bilirubin is less than 5 mg/dL: or (x) the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is less than or equal to 5 times the upper limit of normal (ULN) for the test.

15. The method of claim 14, wherein the patient has an Eastern Cooperative Oncology Group (ECOG) performance status of 0, 1, 2, 3, or 4 at the time of treatment.

16. The method of claim 14, wherein the patient is refractory to or has a recurrence after receiving 2 or more prior systemic therapies.

17. The method of claim 14, wherein the patient has an oxygen saturation level measured via pulse oximeter of at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%.

18. The method of claim 14, wherein the patient has a left ventricular ejection fraction (LVEF) of at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, or 65%.

19. The method of claim 14, wherein the patient has an absolute neutrophil count (ANC) of at least 1000/mm.sup.3 (1.010.sup.9 /L), 1500/mm.sup.3, 2000/mm.sup.3, 2500/mm.sup.3, 3000/mm.sup.3, 3500/mm.sup.3. 4000/mm.sup.3, 4500/mm.sup.3, 5000/mm.sup.3, 5500/mm.sup.3, or 6000/mm.sup.3.

20. The method of claim 14, wherein the patient has a platelet count of at least 75000/mm.sup.3 (7510.sup.9 /L), 100000/mm.sup.3, 125000/mm.sup.3, 150000/mm.sup.3, 175000/mm.sup.3, 200000/mm.sup.3, 225000/mm.sup.3, 250000/mm.sup.3, 275000/mm.sup.3, 300000/mm.sup.3, 325000/mm.sup.3, 350000/mm.sup.3, 375000/mm.sup.3, or 400000/mm.sup.3.

21. The method of claim 14, wherein the patient's hemoglobin level, with or without prior transfusion, is at least 8.0, 9.0, 10.0, 11.0, 12.0, 130, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, or 20.0 g/dL

22. The method of claim 14, wherein the patient's creatinine clearance is at least 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120. 125, 130, 135, or 140 mL/min using the Cockcraft-Gault equation, or mL/min/1.73 m.sup.2 as estimated glomerular filtration rate (eGFR) per Modification of Diet in Renal Disease (MDRD) equation.

23. The method of claim 14, wherein the patient's total serum bilirubin is less than 5, 4.5, 4.0, 3.5, 3.0, 2.5, 2.0, 1.5, 1.0, 0.5, 0.4, 0.3, 0.2, or 0.1 mg/dL.

24. The method of claim 14, wherein the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is less than or equal to 5, 4.5, 4.0, 3.5, 3.0, 2.5, 2.0, 1.5, 1.0, 0.5, or 0 times the upper limit of normal (ULN) for the test.

25. A method comprising administering NK cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15, wherein the patient meets one or more of the following criteria: (i) the patient does not have active nervous system metastases: (ii) the patient does not have a history or presence of a clinically relevant CNS disorder such as a seizure disorder (e.g., epilepsy), cerebrovascular ischemia/hemorrhage, dementia, cerebellar disease, cerebral edema, posterior reversible encephalopathy syndrome (PRES), or any autoimmune disease with CNS involvement: (iii) the patient does not have active human immunodeficiency virus (HIV) infection, e.g., as evidenced by a positive HIV polymerase chain reaction (PCR) test; (iv) the patient does not have active HBV or hepatitis C virus (HCV) infection, e.g., based on laboratory testing: (v) the patient has a viral HBV load below the institutional limit of quantification (LOQ) and the subject is on stable viral suppressive therapy: or (vi) the patient has a HCV RNA viral load below the institutional LOQ and has completed curative antiviral treatment.

26. A method comprising administering NK cells to a patient in need thereof, wherein the NK cells comprise a polynucleotide comprising: a) a nucleic acid encoding an anti-human epidermal growth factor receptor 2 (HER2) chimeric antigen receptor (CAR) comprising an extracellular antigen binding domain comprising an anti-HER2 antibody or antigen binding fragment thereof; and b) a nucleic acid encoding an IL-15, wherein the patient meets one or more of the following criteria: (i) the patient is diagnosed with or has been diagnosed with a HER2+ cancer with an IHC score of 2+; (ii) the patient has a cancer that is not HER2-addicted, but shows some degree of HER2-expression: (iii) the patient is diagnosed with or has been diagnosed with a HER2-low cancer: (iv) the patient is diagnosed with or has been diagnosed with a HER2-low cancer with a HER2 expression classification of score 1+ or 2+; (v) the patient is diagnosed with or has been diagnosed with a HER2-low cancer without HER2 amplification; (vi) the patient has a cancer comprising a HER2 activating mutation; or (vii) the patient has been diagnosed with a HER2 expressing cancer that is IHC 1+ or IHC 2+/ISH and is refractory to or has a recurrence after receiving previous treatment.

27. The method of claim 26, wherein the patient is diagnosed with or has been diagnosed with a HER2-low cancer with a HER2 expression classification of score 1+ or 2+without HER2 amplification.

28. The method of claim 27, wherein the HER2-low cancer harbors HER2 gain.

29. The method of claim 27, wherein the HER2-low cancer lacks HER2 gain.

30. The method of claim 26, wherein the patient has been diagnosed with a IHC 3+ or IHC 2+/ISH+ cancer and is refractory to or has a recurrence after receiving previous treatment with a HER2-targeting therapy.

31. The method of claim 26, wherein the patient has been diagnosed with a HER2 expressing cancer that is IHC 1+ or IHC 2+/ISH, and the patient's cancer is unresectable, metastatic, or the patient is refractory to or has a recurrence after receiving previous treatment.

32. The method of any one of claims 1 to 31, wherein the anti-HER2 antibody or antigen binding fragment thereof comprises a light chain complementarity determining region 1 (CDRL1) comprising SEQ ID NO: 34, a light chain complementarity determining region 2 (CDRL2) comprising SEQ ID NO: 36; a light chain complementarity determining region 3 (CDRL3) comprising SEQ ID NO: 38, a heavy chain complementarity determining region 1 (CDRH1 comprising SEQ ID NO: 44; a heavy chain complementarity determining region 2 (CDRH2) comprising SEQ ID NO: 46; and a heavy chain complementarity determining region 3 (CDRH3) comprising SEQ ID NO: 48.

33. The method of any one of claims 1-32, wherein the nucleic acid encoding the anti-HER2 antibody or antigen binding fragment thereof encodes a CDRL1 encoded by SEQ ID NO: 35, a CDRL2 encoded by SEQ ID NO: 37; a CDRL3 encoded by SEQ ID NO: 39, a CDRH1 encoded by SEQ ID NO: 45: a CDRH2 encoded by SEQ ID NO: 47; and a CDRH3 encoded by SEQ ID NO: 49.

34. The method of any one of claims 1-33, wherein the anti-HER2 antibody or antigen binding fragment thereof comprises a light chain variable (VL) region comprising SEQ ID NO: 32 and a heavy chain variable (V.sub.H) region comprising SEQ ID NO: 42.

35. The method of any one of claims 1-34, wherein the nucleic acid encoding the anti-HER2 antibody or antigen binding fragment thereof comprises a nucleic acid encoding a VL region comprising SEQ ID NO: 33 and a nucleic acid encoding a V.sub.H region comprising SEQ ID NO: 37.

36. The method of any one of claims 1-35, wherein the anti-HER2 antibody or antigen binding fragment thereof comprises a VL region comprising an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 32 and a V.sub.H region comprising an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 42.

37. The method of any one of claims 1-36, wherein the anti-HER2 antibody or antigen binding fragment thereof is an antigen binding fragment.

38. The method of any one of claims 1-37, wherein the antigen binding fragment comprises a single chain Fv (scFv).

39. The method of any one of claims 1-38, wherein the VL region is amino-terminal to the V.sub.H region.

40. The method of any one of claims 1-38, wherein the VL region is carboxy-terminal to the V.sub.H region.

41. The method of any one of claims 1-40, wherein the VI. region is joined to the V.sub.H region via a flexible linker.

42. The method of claim 41, wherein the flexible linker comprises the amino acid sequence set forth in SEQ ID NO: 40.

43. The method of claim 42, wherein the flexible linker is encoded by a nucleic acid comprising SEQ ID NO: 41.

44. The method of any one of claims 38-43, wherein the scFv comprises the amino acid sequence set forth in SEQ ID NO: 30.

45. The method of claim 44, wherein the scFv is encoded by a nucleic acid comprising SEQ ID NO: 31.

46. The method of claim 44, wherein the scFv comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 30.

47. The method of any one of claims 1-46, wherein the anti-HER2 CAR specifically binds to a human epidermal growth factor receptor 2 (HER2) protein.

48. The method of claim 47, wherein the HER2 protein comprises the amino acid sequence of SEQ ID NO: 62.

49. The method of any one of claims 1-48, wherein the CAR comprises a transmembrane domain, optionally a CD28 transmembrane domain.

50. The method of claim 49, wherein the CD28 transmembrane domain comprises the amino acid sequence set forth in SEQ ID NO: 53.

51. The method of claim 50, wherein the CD28 transmembrane domain is encoded by a nucleic acid comprising the nucleic acid sequence set forth in SEQ ID NO: 54or SEQ ID NO: 55.

52. The method of any one of claims 1-51, wherein the CAR further comprising a hinge domain between the extracellular antigen binding domain and the transmembrane domain.

53. The method of claim 52, wherein the hinge domain comprises at least a portion of a CD8a hinge domain.

54. The method of claim 53, wherein the CD8a hinge domain comprises an amino acid sequence set forth in SEQ ID NO: 50.

55. The method of claim 54, wherein the CD8a hinge domain is encoded by a nucleic acid comprising SEQ ID NO: 51or SEQ ID NO: 52.

56. The method of claim 54, wherein the CD8a hinge domain comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 50.

57. The method of any one of claims 1-56, wherein the CAR comprises an intracellular signaling region, optionally where the intracellular signaling region comprises a CD28 intracellular signaling domain, an OX40L intracellular signaling domain, and a CD3-zeta (CD3) signaling domain.

58. The method of claim 57, wherein the intracellular signaling region comprises a CD28 intracellular signaling domain and a CD3-zeta signaling domain.

59. The method of any one of claim 57 or 58, wherein the intracellular signaling region comprises an OX40L intracellular signaling domain.

60. The method of claim 57 or claim 59, wherein the OX40L intracellular signaling domain comprises an amino acid sequence set forth in SEQ ID NO: 8. SEQ ID NO: 9, or SEQ ID NO: 10.

61. The method of claim 57 or claim 59, wherein the OX40L intracellular signaling domain comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10.

62. The method of claim 57 or claim 59, wherein the OX40L intracellular signaling domain is encoded by a nucleic acid comprising SEQ ID NO: 11 or SEQ ID NO: 12.

63. The method of any one of claims 57-62, wherein the intracellular signaling region comprises a CD28 intracellular signaling domain.

64. The method of claim 63, wherein the CD28 intracellular signaling domain comprises an amino acid sequence set forth in SEQ ID NO: 5.

65. The method of claim 63, wherein the CD28 intracellular signaling domain comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 5.

66. The method of claim 63, wherein the CD28 intracellular signaling domain is encoded by a nucleic acid comprising SEQ ID NO: 6 or SEQ ID NO: 7.

67. The method of any one of claims 57-66, wherein the intracellular signaling region comprises an CD3-zeta intracellular signaling domain.

68. The method of claim 67, wherein the CD3-zeta intracellular signaling domain comprises an amino acid sequence set forth in SEQ ID NO: 13.

69. The method of claim 67, wherein the CD3-zeta intracellular signaling domain comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 13.

70. The method of claim 67, wherein the CD3-zeta intracellular signaling domain is encoded by a nucleic acid comprising SEQ ID NO: 14 or SEQ ID NO: 15.

71. The method of any one of claims 57-70, wherein the intracellular signaling region comprises an amino acid sequence set forth in SEQ ID NO: 25.

72. The method of any one of claims 57-70, wherein the intracellular signaling region comprises an amino acid sequence having or having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identity to SEQ ID NO: 25.

73. The method of any one of claims 1-72, wherein the CAR comprises an amino sequence set forth in SEQ ID NO: 56.

74. The method of claim 73, wherein the CAR is encoded by a nucleic acid comprising SEQ ID NO: 57.

75. The method of any one of claims 1-74, wherein the IL-15 comprises the amino acid sequence set forth in SEQ ID NO: 22.

76. The method of claim 75, wherein the IL-15 is encoded by a nucleic acid comprising SEQ ID NO: 23 or SEQ ID NO: 24.

77. The method of any one of claims 1-76, wherein the polynucleotide encodes a polyprotein comprising the CAR and the IL-15.

78. The method of any one of claims 1-77, wherein the polynucleotide further comprises a nucleic acid encoding a self-cleaving peptide, optionally a T2A self-cleaving peptide.

79. The method of claim 78, wherein the CAR is joined to the IL-15 by the self-cleaving peptide.

80. The method of claim 79, wherein the self-cleaving peptide is capable of inducing ribosomal skipping between the CAR and the IL-15.

81. The method of any one of claims 1-80, wherein the polynucleotide further comprises a nucleic acid encoding a signal sequence.

82. The method of claim 81, wherein the signal sequence comprises the amino acid sequence set forth in SEQ ID NO: 27.

83. The method of claim 81, wherein the nucleic acid encoding the signal sequence comprises the nucleic acid sequence set forth in SEQ ID NO: 28.

84. The method of any one of claims 1-83, wherein the polynucleotide encodes a polyprotein comprising the amino acid sequence set forth in SEQ ID NO: 59.

85. The method of any one of claims 1-84, wherein the polynucleotide comprises the nucleic acid sequence set forth in SEQ ID NO: 60 or SEQ ID NO: 61.

86. The method of any one of claims 1-85, wherein the NK cells are cord blood NK cells.

87. The method of any one of claims 1-86, wherein the NK cells comprise a KIR-B haplotype.

88. The method of any one of claims 1-87, wherein the NK cells express CD16 having the V/V polymorphism at F158.

89. The method of any one of claims 1-88, wherein the NK cells are administered as part of a pharmaceutical composition.

90. The method of claim 89, wherein the pharmaceutical composition comprises a pharmaceutically acceptable excipient.

91. The method of claim 89, wherein the pharmaceutical composition comprises: (a) human albumin; (b) dextran; (c) glucose; (d) DMSO; and (e) a buffer.

92. The method of claim 91, wherein the pharmaceutical composition comprises from 30 to 50 mg/ml human albumin.

93. The method of claim 91, wherein the pharmaceutical composition comprises 40 mg/ml human albumin.

94. The method of any one of claims 91-93, wherein the pharmaceutical composition comprises 20 to 30 mg/mL dextran.

95. The method of any one of claims 91-94, wherein the pharmaceutical composition comprises 25 mg/mL dextran.

96. The method of any one of claims 91-95, wherein the dextran is Dextran 40.

97. The method of any one of claims 91-96, wherein the pharmaceutical composition comprises from 12 to 15 mg/mL glucose.

98. The method of any one of claims 91-97, wherein the pharmaceutical composition comprises 12.5 mg/mL glucose.

99. The method of any one of claims 91-98, wherein the pharmaceutical composition comprises less than 27.5 g/L glucose.

100. The method of any one of claims 91-99, wherein the pharmaceutical composition comprises from 50 to 60 ml/mL DMSO.

101. The method of any one of claims 91-100, wherein the pharmaceutical composition comprises 55 mg/mL DMSO.

102. The method of any one of claims 91-101, wherein the pharmaceutical composition comprises 40 to 60% v/v buffer.

103. The method of any one of claims 91-102, wherein the buffer is phosphate buffered saline.

104. The method of claim 91, wherein the pharmaceutical composition comprises: (a) about 40 mg/mL human albumin; (b) about 25 mg/ml Dextran 40; (c) about 12.5 mg/mL glucose; (d) about 55 mg/mL DMSO; and (e) about 0.5 mL/mL phosphate buffered saline.

105. The method of any one of claims 89-104, wherein the pharmaceutical composition further comprises 0.5 mL/mL water.

106. The method of any one of claims 1-85, wherein the subject has a disease or condition associated with HER2.

107. The method of any one of the preceding claims, wherein the disease or condition associated with HER2 is cancer.

108. The method of claim 107, wherein the cancer expresses HER2.

109. The method of claim 87, wherein the cancer is a HER2+ cancer.

110. The method of claim 108 or 109, wherein the cancer is or comprises a solid tumor.

111. The method of any one of claims 107-109, wherein the cancer is or comprises a bladder cancer, breast adenocarcinoma, colorectal adenocarcinoma, non-small cell lung cancer, esophageal cancer, cervix squamous cancer, stomach adenocarcinoma, cholangiocarcinoma, ovary cancer, renal papillary cell carcinoma, and combinations thereof.

112. The method of any one of claims 107-109, wherein the cancer is or comprises a breast cancer.

113. The method of any one of claims 107-109, wherein the breast cancer is noninvasive.

114. The method of any one of claims 107-109, wherein the breast cancer is invasive.

115. The method of any one of claims 107-109, wherein the breast cancer is metastatic.

116. The method of any one of claims 107-109, wherein the cancer is or comprises a gastric cancer.

117. The method of any one of claims 107-109, wherein the cancer is or comprises an ovarian cancer.

118. The method of any one of claims 107-109, wherein the cancer is or comprises a gastroesophageal cancer.

119. The method of any one of claims 107-109, wherein the cancer is or comprises a lung cancer.

120. The method of claim 119, wherein the lung cancer is non-small cell lung cancer (NSCLC).

121. The method of claim 120, wherein the patient has a HER2 activating mutation.

122. The method or use of any one of the foregoing claims, further comprising administering a lymphodepleting chemotherapy to the subject prior to treatment.

123. The method of claim 122, wherein the lymphodepleting chemotherapy is non-myeloablative chemotherapy.

124. The method of claim 122 or claim 123, wherein the lymphodepleting chemotherapy comprises treatment with at least one of cyclophosphamide and fludarabine.

125. The method of claim 124, wherein the lymphodepleting chemotherapy comprises treatment with cyclophosphamide and fludarabine.

126. The method of any one of claims 124-125, wherein between 100 and 500 mg/m.sup.2 cyclophosphamide is administered per day.

127. The method of claim 126, wherein 250 mg/m.sup.2 cyclophosphamide is administered per day.

128. The method of claim 126, wherein 500 mg/m.sup.2 cyclophosphamide is administered per day.

129. The method of any one of claims 124-128, wherein between 10 and 50 mg/m.sup.2 of fludarabine is administered per day.

130. The method of claim 129, wherein 30 mg/m.sup.2 of fludarabine is administered per day.

131. The method or use of any one of claims 106-130, further comprising administering IL-2 to the subject.

132. The method of claim 131, wherein the patient is administered 110.sup.6IU/m.sup.2 of IL-2.

133. The method of claim 131, wherein the patient is administered 110.sup.7 IU of IL-2.

134. The method of claim 131, wherein the patient is administered 610.sup.7 IU of IL-2.

135. The method of any one of claims 132-134, wherein administration of IL-2 occurs within 1-4 hours of administration of the cells.

136. The method of any one of claims 132-134, wherein administration of IL-2 occurs at least 1-4 hours after the administration of the cells.

137. The method of any one of claims 106-136, comprising administering the cells a plurality of times.

138. The method of any one of claims 106-137, comprising administering the cells three times, four times, or eight times.

139. The method of any one of claims 106-137, comprising administering the cells every week, every two weeks, every three weeks, or every four weeks.

140. The method of any one of claims 106-139, further comprising administering pertuzumab to the subject.

141. The method of any one of claims 106-140, further comprising administering trastuzumab to the subject.

142. The method of any one of claims 106-141, further comprising administering necitumumab to the subject.

143. The method of any one of claims 106-142, further comprising administering margetuximab to the subject.

144. The method of any one of claims 106-143, further comprising administering taxane to the subject.

145. The method of claim 144, wherein the taxane is at least one of paclitaxel, docetaxel, and cabazitaxel

146. The method of any one of claims 106-145, further comprising administering an endocrine therapy to the subject.

147. The method of claim 146, wherein the endocrine therapy comprises at least one of an aromatase inhibitor, fulvestrant, and tamoxifen.

148. The method of any one of claims 106-147, further comprising administering a checkpoint inhibitor to the subject.

149. The method of claim 148, wherein the checkpoint inhibitor inhibits CTLA-4, PD-1, or PD-L1.

150. The method of claim 148 or claim 149, wherein the checkpoint inhibitor is or comprises ipilimumab.

151. The method of any one of claims 148-150, wherein the checkpoint inhibitor is or comprises nivolumab.

152. The method of any one of claims 148-151, wherein the checkpoint inhibitor is or comprises pembrolizumab.

153. The method of any one of claims 148-152, wherein the checkpoint inhibitor is or comprises cemiplimab.

154. The method of any one of claims 148-153, wherein the checkpoint inhibitor is or comprises atezolizumab.

155. The method of any one of claims 148-150, wherein the checkpoint inhibitor is or comprises avelumab.

156. The method of any one of claims 148-150, wherein the checkpoint inhibitor is or comprises durvalumab.

157. The method of any one of claims 1-156, further comprising: administering a second therapeutic moiety to the subject.

158. The method of claim 157, wherein the second therapeutic moiety comprises a lymphodepleting chemotherapy agent.

159. The method of any one of claims 157-158, wherein the second therapeutic moiety comprises IL-2.

160. The method of any one of claims 157-159, wherein the second therapeutic moiety comprises at least one of pertuzumab, trastuzumab, necitumumab, and margetuximab.

161. The method of any one of claims 157-160, wherein the second therapeutic moiety comprises a taxane.

162. The method of any one of claims 157-161, wherein the second therapeutic moiety comprises a checkpoint inhibitor.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0240] The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:

[0241] FIG. 1 shows an exemplary embodiment of a method for NK cell expansion and stimulation.

[0242] FIG. 2 shows examples of different manufacturing schemes for master cell bank (MCB) and drug product (DP) manufacturing.

[0243] FIG. 3 shows phenotypes of expanded and stimulated population of NK cells.

[0244] FIG. 4 shows that CAR-NKs comprising a co-stimulatory domain comprising OX40L exhibited greater cytotoxic potential than those without OX40L.

[0245] FIG. 5 shows schematics of CAR constructs.

[0246] FIG. 6 shows proliferation of the CAR constructs of FIG. 5.

[0247] FIG. 7 shows CAR expression of the CAR constructs of FIG. 5.

[0248] FIG. 8 shows CD107a expression of the CAR constructs of FIG. 5. Bars, from left to right: Mock, 2nd-CAR, 3rd_CAR.

[0249] FIG. 9 shows IFN- expression of the CAR constructs of FIG. 5. Bars, from left to right: Mock, 2nd-CAR, 3rd-CAR.

[0250] FIG. 10 shows TNF- expression of the CAR constructs of FIG. 5. Bars, from left to right: Mock, 2nd-CAR. 3rd-CAR.

[0251] FIG. 11 shows short term cytotoxicity of the CAR constructs of FIG. 5.

[0252] FIG. 12 shows schemes of CAR constructs. From top to bottom: mock GFP expressing NK (mock-NK); CAR without IL-15 (CAR-NK); truncated CAR with IL-15 (CAR (t)-IL-15-NK): CAR with IL-15 (CAR-IL-15-NK, AB-201)

[0253] FIG. 13 shows CAR expression on NK cells cultured in the presence of IL-2.

[0254] FIG. 14 shows CAR expression on NK cells cultured in the absence of IL-2.

[0255] FIG. 15 shows proliferation of NK cells cultured in the presence of IL-2 and in the absence of IL-2.

[0256] FIG. 16 shows viability of NK cells cultured in the presence of IL-2 and in the absence of IL-2.

[0257] FIG. 17 shows cytotoxicity of NK cells.

[0258] FIG. 18 shows IFNg production of NK cells.

[0259] FIG. 19 shows IL-15 production of NK cells.

[0260] FIG. 20 demonstrates that secretion of IL-15 Maintains the Survival of Bystander NK Cells.

[0261] FIG. 21 is a schematic of two different CAR structures.

[0262] FIG. 22 shows CAR expression of the CAR structures shown in FIG. 21 over time

[0263] (in days).

[0264] FIG. 23 shows survival of cells expressing the CAR structures shown in FIG. 21 (cell numbers).

[0265] FIG. 24 shows survival of cells expressing the CAR structures shown in FIG. 21 (percent viability).

[0266] FIG. 25 shows the number of viable NK cells after 7 days of co-culture of cells expressing the CAR structures shown in FIG. 21 with target cells without IL-2 support. Bars, from left to right: cord blood NK cells (CBNK): 3rd CAR-NK: 4th CAR-NK.

[0267] FIG. 26 shows in vitro killing activity of AB-201 against the breast carcinoma (HER2-) cell line MDA-MB-468.

[0268] FIG. 27 shows in vitro killing activity of AB-201 against the ovarian carcinoma (HER2+) cell line SKOV3.

[0269] FIG. 28 shows in vitro killing activity of AB-201 against the gastric carcinoma (HER2+) cell line NCI-N87.

[0270] FIG. 29 shows in vitro killing activity of AB-201 against the breast carcinoma (HER2+) cell line HCC1954.

[0271] FIG. 30 shows in vitro killing activity of AB-201 against the breast carcinoma

[0272] (HER2+) cell line K562.

[0273] FIG. 31 shows in vitro characterization of AB-201.

[0274] FIG. 32 shows in vitro characterization of AB-201.

[0275] FIG. 33 shows in vitro characterization of AB-201.

[0276] FIG. 34 shows in vitro characterization of AB-201.

[0277] FIG. 35 shows in vitro characterization of AB-201.

[0278] FIG. 36 shows in vitro characterization of AB-201.

[0279] FIG. 37 shows the results of a long-term killing assay on NCI-N87 gastric carcinoma cells in culture using Incucyte live cell imaging. Effector to target ratio (E: T 0.3:1).

[0280] FIG. 38 shows that a single AB-201 administration of one million cells in a HER2+HCC1954 Breast Carcinoma model conferred a substantial survival benefit over trastuzumab.

[0281] FIG. 39 shows that a single AB-201 administration on day four after the establishment of a HER2+ trastuzumab-resistant breast cancer model resulted in tumor regression.

[0282] FIG. 40 shows in vivo characterization of AB-201.

[0283] FIG. 41 shows in vivo characterization of AB-201.

[0284] FIG. 42 shows in vivo characterization of AB-201.

[0285] FIG. 43 shows in vivo characterization of AB-201.

[0286] FIG. 44 shows cytotoxicity of primary cells (non-tumor) measured following co-culture of AB-201 or control CB-NK cells with pulmonary artery endothelial cells. keratinocytes, renal epithelial cells, cardiac myocytes and small airway epithelial cells for 4 hours at Effector: Target (E: T) ratios of 3:1, 1:1, or 0.3:1.

[0287] FIG. 45 shows tumor volume measurements of NSG mice that received SKOV3-Luc tumor cells (IP) and either not treated (open circles, dashed line) or treated with AB-201 (closed circles, solid line). The vertical line on Day 11 depicts the date of the AB-201 injection.

[0288] FIG. 46 shows measurements of body weight in NSG mice that received SKOV3-Luc tumor cells (IP) and either not treated (open circles, dashed line) or treated with AB-201 (closed circles, solid line).

[0289] FIG. 47 shows that AB-201 cells persisted in AB-201-treated mice at detectable levels until at least day 52.

[0290] FIG. 48 shows tumor volume of irradiated mice inoculated with NCI-N87 cells and administered no treatment, cord blood NK cells, or AB-201 cells.

[0291] FIG. 49 shows tumor volume of unconditioned mice inoculated with NCI-N87 cells and administered no treatment, cord blood NK cells, or AB-201 cells.

[0292] FIG. 50 shows body weight measurements of mice depicted in FIG. 48 and FIG. 49.

[0293] FIG. 51 shows that AB-201 infiltrates tumors, as depicted by detection of CD56 by immunofluorescence.

[0294] FIG. 52 shows an experimental design: NSG mice received 110.sup.6 SK-OV-3-Luc tumor cells intraperitoneally (IP) on day 0 and were randomized 4 days later. A single injection of CB-NK (510.sup.6 dose only) or AB-201 (110.sup.6 or 510.sup.6) was administered (IP) on day 5 or on day 5 and 12.

[0295] FIG. 53 shows efficacy of AB-201 in a SK-OV-3-Luc xenograft tumor model.

[0296] FIG. 54 shows body weight change in SK-OV-3-Luc Tumor-Bearing Mice. Body weight change was calculated based on the BW of the mouse on the day the NK cells were injected.

[0297] FIG. 55 shows presence of AB-201 in peripheral lymphoid tissues. AB-201 and CB-NK were measured in peripheral blood and spleen by flow cytometry at indicated timepoints post-tumor inoculation (gated on human CD45+CD56+).

[0298] FIG. 56 shows cell surface marker expression on CBNK or AB-201 after thawing.

[0299] FIG. 57 shows specific cytotoxicity of AB-201 and control CB-NK cells against K562. SK-OV-3. HCC1954, and NCI-N87 at different E: T ratios.

[0300] FIG. 58 shows kinetic analysis of cellular cytotoxicity of AB-201 and CBNK against SK-OV-3, HCC1954, and NCI-N87. Long-term cellular cytotoxicity of AB-201 and control CB-NK cells against HER2+ target tumor cell lines representing different solid tumor malignancies was measured using the Incucyte LiveCell analysis system for 120 hours.

[0301] FIG. 59 shows degranulation and cytokine secretion of AB-201 and CBNK following stimulation by K562, SK-OV-3, HCC1954, and NCI-N87. AB-201 or control CB-NK cells were stimulated for 24 hours with multiple target tumor cells at a 1:1 Effector: Target (E: T) ratio. Following stimulation, cells were collected and characterized for degranulation (CD107a) and cytokine secretion (IFN, TNF) by flow cytometry. Data are expressed as percent positive within the CD56+ gated population. (n=3) (*p<0.05, ** p<0.01, compared with CBNK, two-tailed t-test).

[0302] FIG. 60 Secretion of IFN- by AB-201 following stimulation with K562, SK-OV-3,

[0303] HCC1954, and NCI-N87. AB-201 or control CB-NK cells were stimulated for 24 hours with target tumor cells at a 3:1 Effector: Target (E: T) ratio. Following collection of cell-free supernatants, soluble cytokine levels were measured by ELISA for IFN. (*p<0.05, ** p<0.01, compared with CBNK, two-tailed t-test).

[0304] FIG. 61 shows secretion of IL-15 by AB-201 following stimulation with K562, SK-OV-3, HCC1954, and NCI-N87. AB-201 or control CB-NK cells were stimulated for 24 hours with target tumor cells at a 3:1 Effector: Target (E: T) ratio. Following collection of cell-free supernatants, soluble cytokine levels were measured by ELISA for IL-15. (*p<0.05, compared with CBNK, two-tailed t-test).

DETAILED DESCRIPTION

[0305] Provided herein are, amongst other things, Natural Killer (NK) cells, e.g., CAR-NK cells, methods for producing the NK cells, pharmaceutical compositions comprising the NK cells, and methods of treating patients suffering, e.g., from cancer, with the NK cells. EXPANSION AND STIMULATION OF NATURAL KILLER CELLS

[0306] In some embodiments, natural killer cells are expanded and stimulated, e.g., by culturing and stimulation with feeder cells.

[0307] NK cells can be expanded and stimulated as described, for example, in US 2020/0108096 or WO 2020/101361. both of which are incorporated herein by reference in their entirety. Briefly, the source cells can be cultured on modified HuT-78 (ATCCR TIB-16] ) cells that have been engineered to express 4-1BBL, membrane bound IL-21, and a mutant TNF as described in US 2020/0108096.

[0308] Suitable NK cells can also be expanded and stimulated as described herein.

[0309] In some embodiments, NK cells are expanded and stimulated by a method comprising: (a) providing NK cells, e.g., a composition comprising NK cells, e.g., CD3 () cells; and (b) culturing in a medium comprising feeder cells and/or stimulation factors, thereby producing a population of expanded and stimulated NK cells.

Natural Killer Cell Sources

[0310] In some embodiments, the NK cell source is selected from the group consisting of peripheral blood, peripheral blood lymphocytes (PBLs), peripheral blood mononuclear cells (PBMCs), bone marrow, umbilical cord blood (cord blood), isolated NK cells, NK cells derived from induced pluripotent stem cells, NK cells derived from embryonic stem cells, and combinations thereof.

[0311] In some embodiments, the NK cell source is a single unit of cord blood.

[0312] In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises from or from about 110.sup.7 to or to about 110.sup.9 total nucleated cells. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises from or from about 110.sup.8 to or to about 1.510.sup.8 total nucleated cells. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises 110.sup.8 total nucleated cells. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises about 110.sup.8 total nucleated cells. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises 110.sup.9 total nucleated cells. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises about 110.sup.9 total nucleated cells.

[0313] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises from about 20% to about 80% CD16+ cells. In some embodiments, the NK cell source, e.g., the cord blood unit, comprises from or from about 20% to or to about 80%, from about 20% to or to about 70%, from about 20% to or to about 60%, from about 20% to or to about 50%, from about 20% to or to about 40%, from about 20% to or to about 30%, from about 30% to or to about 80%, from about 30% to or to about 70%, from about 30% to or to about 60%, from about 30% to or to about 50%, from about 30% to or to about 40%, from about 40% to or to about 80%, from about 40% to or to about 70%, from about 40% to or to about 60%, from about 40% to or to about 50%, from about 50% to or to about 80%, from about 50% to or to about 70%, from about 50% to or to about 60%, from about 60% to or to about 80%, from about 60% to or to about 70%, or from about 70% to or to about 80% CD16+ cells. In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 80% CD16+ cells. Alternately, some NK cell sources may comprise CD16+ cells at a concentration of greater than 80%.

[0314] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% MLG2A+ cells.

[0315] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% NKG2C+ cells.

[0316] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% NKG2D+ cells.

[0317] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% NKp46+ cells.

[0318] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% NKp30+ cells.

[0319] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% DNAM-1+ cells.

[0320] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% NKp44+ cells.

[0321] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% CD25+ cells.

[0322] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% CD62L+ cells.

[0323] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% CD69+ cells.

[0324] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% CXCR3+ cells.

[0325] In some embodiments, the NK cell source, e.g., the cord blood unit, comprises less than or equal to 40%, e.g., less than or equal to 30%, e.g., less than or equal to 20%, e.g., less than or equal to 10%, e.g., less than or equal to 5% CD57+ cells.

[0326] In some embodiments, NK cells in the NK cell source comprise a KIR B allele of the KIR receptor family. See, e.g., . Hsu et al., The Killer Cell Immunoglobulin-Like Receptor (KIR) Genomic Region: Gene-Order, Haplotypes and Allelic Polymorphism, Immunological Review 190:40-52 (2002); and Pyo et al., Different Patterns of Evolution in the Centromeric and Telomeric Regions of Group A and B Haplotypes of the Human Killer Cell Ig-like Receptor Locus, PLOS One 5: e15115 (2010).

[0327] In some embodiments, NK cells in the NK cell source comprise the 158 V/V variant of CD16 (i.e. homozygous CD16 158V polymorphism). See, e.g., . Koene et al., FcRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcgammaRIIIa, Independently of the FcgammaRIIIa-48L/R/H Phenotype, Blood 90:1109-14 (1997).

[0328] In some embodiments, NK cells in the cell source comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16.

[0329] In some embodiments, the NK cells in the cell source are not genetically engineered.

[0330] In some embodiments, the NK cells in the cell source do not comprise a CD16 transgene.

[0331] In some embodiments, the NK cells in the cell source do not express an exogenous CD16 protein.

[0332] In some embodiments, the NK cell source is CD3 (+) depleted. In some embodiments, the method comprises depleting the NK cell source of CD3 (+) cells. In some embodiments, depleting the NK cell source of CD3 (+) cells comprises contacting the NK cell source with a CD3 binding antibody or antigen binding fragment thereof. In some embodiments, the CD3 binding antibody or antigen binding fragment thereof is selected from the group consisting of OKT3, UCHT1, and HIT3a, and fragments thereof. In some embodiments, the CD3 binding antibody or antigen binding fragment thereof is OKT3 or an antigen binding fragment thereof. In some embodiments, the antibody or antigen binding fragment thereof is attached to a bead, e.g., a magnetic bead. In some embodiments, the depleting the composition of CD3 (+) cells comprises contacting the composition with a CD3 targeting antibody or antigen binding fragment thereof attached to a bead and removing the bead-bound CD3 (+) cells from the composition. The composition can be depleted of CD3 cells by immunomagnetic selection, for example, using a CliniMACS T cell depletion set ((LS Depletion set (162-01) Miltenyi Biotec).

[0333] In some embodiments, the NK cell source CD56+ enriched, e.g., by gating on CD56 expression.

[0334] In some embodiments, the NK cell source is both CD56+ enriched and CD3 (+) depleted, e.g., by selecting for cells with CD56+CD3-expression.

[0335] In some embodiments, the NK cell source comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and is + enriched and CD3 (+) depleted, e.g., by selecting for cells with CD56+CD3-expression.

Feeder Cells

[0336] Disclosed herein are feeder cells for the expansion of NK cells. These feeder cells advantageously allow NK cells to expand to numbers suitable for the preparation of a pharmaceutical composition as discussed herein. In some cases, the feeder cells allow the expansion of NK cells without the loss of CD16 expression, which often accompanies cell expansion on other types of feeder cells or using other methods. In some cases, the feeder cells make the expanded NK cells more permissive to freezing such that a higher proportion of NK cells remain viable after a freeze/thaw cycle or such that the cells remain viable for longer periods of time while frozen. In some cases, the feeder cells allow the NK cells to retain high levels of cytotoxicity, including ADCC, extend survival, increase persistence, and enhance or retain high levels of CD16. In some cases, the feeder cells allow the NK cells to expand without causing significant levels of exhaustion or senescence.

[0337] Feeder cells can be used to stimulate the NK cells and help them to expand more quickly, e.g., by providing substrate, growth factors, and/or cytokines.

[0338] NK cells can be stimulated using various types of feeder cells, including, but not limited to peripheral blood mononuclear cells (PBMC). Epstein-Barr virus-transformed B-lymphoblastoid cells (e.g., EBV-LCL), myelogenous leukemia cells (e.g., K562), and CD4 (+) T cells (e.g., HuT), and derivatives thereof.

[0339] In some embodiments, the feeder cells are inactivated, e.g., by y-irradiation or mitomycin-c treatment.

[0340] Suitable feeder cells for use in the methods described herein are described, for example, in US 2020/0108096, which is hereby incorporated by reference in its entirety.

[0341] In some embodiments, the feeder cell(s) are inactivated CD4 (+) T cell(s). In some embodiments, the inactivated CD4 (+) T cell(s) are HuT-78 cells (ATCCR TIB-161) or variants or derivatives thereof. In some embodiments, the HuT-78 derivative is H9 (ATCCR) HTB-176).

[0342] In some embodiments, the inactivated CD4 (+) T cell(s) express OX40L. In some embodiments, the inactivated CD4 (+) T cell(s) are HuT-78 cells or variants or derivatives thereof that express OX40L (SEQ ID NO: 4) or a variant thereof.

[0343] In some embodiments, the feeder cells are HuT-78 cells engineered to express at least one gene selected from the group consisting of 4-1BBL (UniProtKB P41273, SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and mutant TNFalpha (SEQ ID NO: 3) (eHut-78 cells), or variants thereof.

[0344] In some embodiments, the inactivated CD4 (+) T cell(s) are HuT-78 (ATCCR TIB-161) cells or variants or derivatives thereof that express an ortholog of OX40L, or variant thereof. In some embodiments, the feeder cells are HuT-78 cells engineered to express at least one gene selected from the group consisting of an 4-1BBL ortholog or variant thereof, a membrane bound IL-21 ortholog or variant thereof, and mutant TNFalpha ortholog, or variant thereof.

[0345] In some embodiments, the feeder cells are HuT-78 cell(s) that express OX40L (SEQ ID NO: 4) and are engineered to express 4-1BBL (SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and mutant TNFalpha (SEQ ID NO: 3) (eHut-78 cells) or variants or derivatives thereof.

[0346] In some embodiments, the feeder cells are expanded, e.g., from a frozen stock, before culturing with NK cells, e.g., as described in Example 2.

Stimulating Factors

[0347] NK cells can also be stimulated using one or more stimulation factors other than feeder cells, e.g., signaling factors, in addition to or in place of feeder cells.

[0348] In some embodiments, the stimulating factor, e.g., signaling factor, is a component of the culture medium, as described herein. In some embodiments, the stimulating factor, e.g., signaling factor, is a supplement to the culture medium, as described herein.

[0349] In some embodiments, the stimulation factor(s) are cytokine(s). In some embodiments, the cytokine(s) are selected from the group consisting of IL-2, IL-12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN-, IFN, and combinations thereof.

[0350] In some embodiments, the cytokine is IL-2.

[0351] In some embodiments, the cytokines are a combination of IL-2 and IL-15.

[0352] In some embodiments, the cytokines are a combination of IL-2, IL-15, and IL-18.

[0353] In some embodiments, the cytokines are a combination of IL-2, IL-18, and IL-21.

Culturing

[0354] The NK cells can be expanded and stimulated by co-culturing an NK cell source and feeder cells and/or other stimulation factors. Suitable NK cell sources, feeder cells, and stimulation factors are described herein.

[0355] In some cases, the resulting population of expanded natural killer cells is enriched and/or sorted after expansion. In some cases, the resulting population of expanded natural killer cells is not enriched and/or sorted after expansion

[0356] Also described herein are compositions comprising the various culture compositions described herein, e.g., comprising NK cells. For example, a composition comprising a population of expanded cord blood-derived natural killer cells comprising a KIR-B haplotype and homozygous for a CD16 158V polymorphism and a plurality of engineered HuT78 cells.

[0357] Also described herein are vessels, e.g., vials, cryobags, and the like, comprising the resulting populations of expanded natural killer cells. In some cases, a plurality of vessels comprising portions of the resulting populations of expanded natural killer cells, e.g., at least 10, e.g., 20. 30. 40, 50, 60, 70, 80, 90, 100, 125, 150, 175, 200, 250, 300, 400, 500, 600, 700, 800. 900, 1000, 1100, or 1200 vessels.

[0358] Also described herein are bioreactors comprising the various culture compositions described herein, e.g., comprising NK cells. For example, a culture comprising natural killer cells from a natural killer cell source, e.g., as described herein, and feeder cells, e.g., as described herein. Also described herein are bioreactors comprising the resulting populations of expanded natural killer cells.

Culture Medium

[0359] Disclosed herein are culture media for the expansion of NK cells. These culture media advantageously allow NK cells to expand to numbers suitable for the preparation of a pharmaceutical composition as discussed herein. In some cases, the culture media allows NK cells to expand without the loss of CD16 expression that often accompanies cell expansion on other helper cells or in other media.

[0360] In some embodiments, the culture medium is a basal culture medium, optionally supplemented with additional components, e.g., as described herein.

[0361] In some embodiments, the culture medium, e.g., the basal culture medium, is a serum-free culture medium. In some embodiments, the culture medium, e.g., the basal culture medium, is a serum-free culture medium supplemented with human plasma and/or serum.

[0362] Suitable basal culture media include, but are not limited to, DMEM, RPMI 1640, MEM, DMEM/F12, SCGM (CellGenix (R), 20802-0500 or 20806-0500), LGM-3 (Lonza, CC-3211), TexMACS (Miltenyi Biotec, 130-097-196), AlyS 505NK-AC (Cell Science and Technology Institute, Inc., 01600P02), AlyS 505NK-EX (Cell Science and Technology Institute, Inc., 01400P10), CTS AIM-V SFM (ThermoFisher Scientific, A3830801), CTS OpTmizer (ThermoFisher Scientific, A1048501, ABS-001, StemXxVivoand combinations thereof.

[0363] The culture medium may comprise additional components, or be supplemented with additional components, such as growth factors, signaling factors, nutrients, antigen binders, and the like. Supplementation of the culture medium may occur by adding each of the additional component or components to the culture vessel either before, concurrently with, or after the medium is added to the culture vessel. The additional component or components may be added together or separately. When added separately, the additional components need not be added at the same time.

[0364] In some embodiments, the culture medium comprises plasma, e.g., human plasma. In some embodiments, the culture medium is supplemented with plasma, e.g., human plasma. In some embodiments, the plasma, e.g., human plasma, comprises an anticoagulant, e.g., trisodium citrate.

[0365] In some embodiments, the medium comprises and/or is supplemented with from or from about 0.5% to or to about 10% v/v plasma, e.g., human plasma. In some embodiments, the medium is supplemented with from or from about 0.5% to or to about 9%, from or from about 0.5% to or to about 8%, from or from about 0.5% to or to about 7%, from or from about 0.5% to or to about 6%, from or from about 0.5% to or to about 5%, from or from about 0.5% to or to about 4%, from or from about 0.5% to or to about 3%, from or from about 0.5% to or to about 2%, from or from about 0.5% to or to about 1%, from or from about 1% to or to about 10%, from or from about 1% to or to about 9%, from or from about 1% to or to about 8%, from or from about 1% to or to about 7%, from or from about 1% to or to about 6%, from or from about 1% to or to about 5%, from or from about 1% to or to about 4%, from or from about 1% to or to about 3%, from or from about 1% to or to about 2%, from or from about 2% to or to about 10%, from or from about 2% to or to about 9%, from or from about 2% to or to about 8%, from or from about 2% to or to about 7%, from or from about 2% to or to about 6%, from or from about 2% to or to about 5%, from or from about 2% to or to about 4%, from or from about 2% to or to about 3%, from or from about 3% to or to about 10%, from or from about 3% to or to about 9%, from or from about 3% to or to about 8%, from or from about 3% to or to about 7%, from or from about 3% to or to about 6%, from or from about 3% to or to about 5%, from or from about 3% to or to about 4%, from or from about 4% to or to about 10%, from or from about 4% to or to about 9%, from or from about 4% to or to about 8%, from or from about 4% to or to about 7%, from or from about 4% to or to about 6%, from or from about 4% to or to about 5%, from or from about 5% to or to about 10%, from or from about 5% to or to about 9%, from or from about 4% to or to about 8%, from or from about 5% to or to about 7%, from or from about 5% to or to about 6%, from or from about 6% to or to about 10%, from or from about 6% to or to about 9%, from or from about 6% to or to about 8%, from or from about 6% to or to about 7%, from or from about 7% to or to about 10%, from or from about 7% to or to about 9%, from or from about 7% to or to about 8%, from or from about 8% to or to about 10%, from or from about 8% to or to about 9%, or from or from about 9% to or to about 10% v/v plasma, e.g., human plasma. In some embodiments, the culture medium comprises and/or is supplemented with from 0.8% to 1.2% v/v human plasma. In some embodiments, the culture medium comprises and/or is supplemented with 1.0% v/v human plasma. In some embodiments, the culture medium comprises and/or is supplemented with about 1.0% v/v human plasma.

[0366] In some embodiments, the culture medium comprises serum, e.g., human serum. In some embodiments, the culture medium is supplemented with serum, e.g., human serum. In some embodiments, the serum is inactivated, e.g., heat inactivated. In some embodiments, the serum is filtered, e.g., sterile-filtered.

[0367] In some embodiments, the culture medium comprises glutamine. In some embodiments, the culture medium is supplemented with glutamine. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 2.0 to or to about 6.0 mM glutamine. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 2.0 to or to about 5.5, from or from about 2.0 to or to about 5.0, from or from about 2.0 to or to about 4.5, from or from about 2.0 to or to about 4.0, from or from about 2.0 to or to about 3.5, from or from about 2.0 to or to about 3.0, from or from about 2.0 to or to about 2.5, from or from about 2.5 to or to about 6.0, from or from about 2.5 to or to about 5.5, from or from about 2.5 to or to about 5.0, from or from about 2.5 to or to about 4.5, from or from about 2.5 to or to about 4.0, from or from about 2.5 to or to about 3.5, from or from about 2.5 to or to about 3.0, from or from about 3.0 to or to about 6.0, from or from about 3.0 to or to about 5.5, from or from about 3.0 to or to about 5.0, from or from about 3.0 to or to about 4.5, from or from about 3.0 to or to about 4.0, from or from about 3.0 to or to about 3.5, from or from about 3.5 to or to about 6.0, from or from about 3.5 to or to about 5.5, from or from about 3.5 to or to about 5.0, from or from about 3.5 to or to about 4.5, from or from about 3.5 to or to about 4.0, from or from about 4.0 to or to about 6.0, from or from about 4.0 to or to about 5.5, from or from about 4.0 to or to about 5.0, from or from about 4.0 to or to about 4.5, from or from about 4.5 to or to about 6.0, from or from about 4.5 to or to about 5.5, from or from about 4.5 to or to about 5.0, from or from about 5.0 to or to about 6.0, from or from about 5.0 to or to about 5.5, or from or from about 5.5 to or to about 6.0 mM glutamine. In some embodiments, the culture medium comprises and/or is supplemented with from 3.2 mM glutamine to 4.8 mM glutamine. In some embodiments, the culture medium comprises and/or is supplemented with 4.0 mM glutamine. In some embodiments, the culture medium comprises and/or is supplemented with about 4.0 mM glutamine.

[0368] In some embodiments, the culture medium comprises one or more cyotkines. In some embodiments, the culture medium is supplemented with one or more cyotkines.

[0369] In some embodiments, the cytokine is selected from IL-2, IL-12, IL-15, IL-18, and combinations thereof.

[0370] In some embodiments, the culture medium comprises and/or is supplemented with IL-2. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 150 to or to about 2,500 IU/mL IL-2. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 200 to or to about 2,250, from or from about 200 to or to about 2,000, from or from about 200 to or to about 1,750, from or from about 200 to or to about 1,500, from or from about 200 to or to about 1,250, from or from 200 to or to about 1,000, from or from about 200 to or to about 750, from or from about 200 to or to about 500, from or from about 200 to or to about 250, from or from about 250 to or to about 2,500, from or from about 250 to or to about 2,250, from or from about 250 to or to about 2,000, from or from about 250 to or to about 1,750, from or from about 250 to or to about 1,500, from or from about 250 to or to about 1,250, from or from about 250 to or to about 1,000, from or from about 250 to or to about 750, from or from about 250 to or to about 500, from or from about 500 to or to about 2,500, from or from about 500 to or to about 2,250, from or from about 500 to or to about 2,000, from or from about 500 to or to about 1,750, from or from about 500 to or to about 1,500, from or from about 500 to or to about 1,250, from or from about 500 to or to about 1,000, from or from about 500 to or to about 750, from or from about 750 to or to about 2,250, from or from about 750 to or to about 2,000, from or from about 750 to or to about 1,750, from or from about 750 to or to about 1,500, from or from about 750 to or to about 1,250, from or from about 750 to or to about 1,000, from or from about 1,000 to or to about 2,500, from or from about 1,000 to or to about 2,250, from or from about 1,000 to or to about 2,000, from or from about 1,000 to or to about 1,750, from or from about 1,000 to or to about 1,500, from or from about 1,000 to or to about 1,250, from or from about 1,250 to or to about 2,500, from or from about 1,250 to or to about 2,250, from or from about 1,250 to or to about 2,000, from or from about 1.250 to or to about 1.750, from or from about 1.250 to or to about 1.500, from or from about 1,500 to or to about 2,500, from or from about 1,500 to or to about 2,250, from or from about 1,500 to or to about 2,000, from or from about 1,500 to or to about 1,750, from or from about 1,750 to or to about 2,500, from or from about 1,750 to or to about 2,250, from or from about 1,750 to or to about 2,000, from or from about 2,000 to or to about 2,500, from or from about 2,000 to or to about 2,250, or from or from about 2,250 to or to about 2,500 IU/mL IL-2.

[0371] In some embodiments, the culture medium comprises and/or is supplemented with from 64 g/L to 96 g/L IL-2. In some embodiments, the culture medium comprises and/or is supplemented with 80 g/L IL-2 (approximately 1,333 IU/mL). In some embodiments, the culture medium comprises and/or is supplemented with about 80 g/L.

[0372] In some embodiments, the culture medium comprises and/or is supplemented with a combination of IL-2 and IL-15.

[0373] In some embodiments, the culture medium comprises and/or is supplemented with a combination of IL-2, IL-15, and IL-18.

[0374] In some embodiments, the culture medium comprises and/or is supplemented with a combination of IL-2, IL-18, and IL-21.

[0375] In some embodiments, the culture medium comprises and/or is supplemented with glucose. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5 to or to about 3.5 g/L glucose. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5 to or to about 3.0, from or from about 0.5 to or to about 2.5, from or from about 0.5 to or to about 2.0, from or from about 0.5 to or to about 1.5, from or from about 0.5 to or to about 1.0, from or from about 1.0 to or to about 3.0, from or from about 1.0 to or to about 2.5, from or from about 1.0 to or to about 2.0, from or from about 1.0 to or to about 1.5, from or from about 1.5 to or to about 3.0, from or from about 1.5 to or to about 2.5, from or from about 1.5 to or to about 2.0, from or from about 2.0 to or to about 3.0, from or from about 2.0 to or to about 2.5, or from or from about 2.5 to or to about 3.0 g/L glucose. In some embodiments, the culture medium comprises and/or is supplemented with from 1.6 to 2.4 g/L glucose. In some embodiments, the culture medium comprises and/or is supplemented with 2.0 g/L glucose. In some embodiments, the culture medium comprises about 2.0 g/L glucose.

[0376] In some embodiments, the culture medium comprises and/or is supplemented with sodium pyruvate. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.1 to or to about 2.0 mM sodium pyruvate. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.1 to or to about 1.8, from or from about 0.1 to or to about 1.6, from or from about 0.1 to or to about 1.4, from or from about 0.1 to or to about 1.2, from or from about 0.1 to or to about 1.0, from or from about 0.1 to or to about 0.8, from or from about 0.1 to or to about 0.6, from or from about 0.1 to or to about 0.4, from or from about 0.1 to or to about 0.2, from or from about 0.2 to or to about 2.0, from or from about 0.2 to or to about 1.8, from or from about 0.2 to or to about 1.6, from or from about 0.2 to or to about 1.4, from or from about 0.2 to or to about 1.2, from or from about 0.2 to or to about 1.0, from or from about 0.2 to or to about 0.8, from or from about 0.2 to or to about 0.6, from or from about 0.2 to or to about 0.4, from or from about 0.4 to or to about 2.0, from or from about 0.4 to or to about 1.8, from or from about 0.4 to or to about 1.6, from or from about 0.4 to or to about 1.4, from or from about 0.4 to or to about 1.2, from or from about 0.4 to or to about 1.0, from or from about 0.4 to or to about 0.8, from or from about 0.4 to or to about 0.6, from or from about 0.6 to or to about 2.0, from or from about 0.6 to or to about 1.8, from or from about 0.6 to or to about 1.6, from or from about 0.6 to or to about 1.4, from or from about 0.6 to or to about 1.2, from or from about 0.6 to or to about 1.0, from or form about 0.6 to or to about 0.8, from or from about 0.8 to or to about 2.0, from or from about 0.8 to or to about 1.8, from or from about 0.8 to or to about 1.6, from or from about 0.8 to or to about 1.4, from or from about 0.8 to or to about 1.4, from or from about 0.8 to or to about 1.2, from or from about 0.8 to or to about 1.0, from or from about 1.0 to or to about 2.0, from or from about 1.0 to or to about 1.8, from or from about 1.0 to or to about 1.6, from or from about 1.0 to or to about 1.4, from or from about 1.0 to or to about 1.2, from or from about 1.2 to or to about 2.0, from or from about 1.2 to or to about 1.8, from or from about 1.2 to or to about 1.6, from or from about 1.2 to or to about 1.4, from or from about 1.4 to or to about 2.0, from or from about 1.4 to or to about 1.8, from or from about 1.4 to or to about 1.6, from or from about 1.6 to or to about 2.0, from or from about 1.6 to or to about 1.8, or from or from about 1.8 to or to about 2.0 mM sodium pyruvate. In some embodiments, the culture medium comprises from 0.8 to 1.2 mM sodium pyruvate. In some embodiments, the culture medium comprises 1.0 mM sodium pyruvate. In some embodiments, the culture medium comprises about 1.0 mM sodium pyuruvate.

[0377] In some embodiments, the culture medium comprises and/or is supplemented with sodium hydrogen carbonate. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5 to or to about 3.5 g/L sodium hydrogen carbonate. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5 to or to about 3.0, from or from about 0.5 to or to about 2.5, from or from about 0.5 to or to about 2.0, from or from about 0.5 to or to about 1.5, from or from about 0.5 to or to about 1.0, from or from about 1.0 to or to about 3.0, from or from about 1.0 to or to about 2.5, from or from about 1.0 to or to about 2.0, from or from about 1.0 to or to about 1.5, from or from about 1.5 to or to about 3.0, from or from about 1.5 to or to about 2.5, from or from about 1.5 to or to about 2.0, from or from about 2.0 to or to about 3.0, from or from about 2.0 to or to about 2.5, or from or from about 2.5 to or to about 3.0 g/L sodium hydrogen carbonate. In some embodiments, the culture medium comprises and/or is supplemented with from 1.6 to 2.4 g/L sodium hydrogen carbonate. In some embodiments, the culture medium comprises and/or is supplemented with 2.0 g/L sodium hydrogen carbonate. In some embodiments, the culture medium comprises about 2.0 g/L sodium hydrogen carbonate.

[0378] In some embodiments, the culture medium comprises and/or is supplemented with albumin, e.g., human albumin, e.g., a human albumin solution described herein. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5% to or to about 3.5% v/v of a 20% albumin solution, e.g., a 20% human albumin solution. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.5% to or to about 3.0%, from or from about 0.5% to or to about 2.5%, from or from about 0.5% to or to about 2.0%, from or from about 0.5% to or to about 1.5%, from or from about 0.5% to or to about 1.0%, from or from about 1.0% to or to about 3.0%, from or from about 1.0% to or to about 2.5%, from or from about 1.0% to or to about 2.0%, from or from about 1.0% to or to about 1.5%, from or from about 1.5% to or to about 3.0%, from or from about 1.5% to or to about 2.5%, from or from about 1.5% to or to about 2.0%, from or from about 2.0% to or to about 3.0%, from or from about 2.0% to or to about 2.5%, or from or from about 2.5% to or to about 3.0% v/v of a 20% albumin solution, e.g., a 20% human albumin solution. In some embodiments, the culture medium comprises and/or is supplemented with from 1.6% to 2.4% v/v of a 20% albumin solution, e.g., a 20% human albumin solution. In some embodiments, the culture medium comprises and/or is supplemented with 2.0% v/v of a 20% albumin solution, e.g., a 20% human albumin solution. In some embodiments, the culture medium comprises about 2.0% v/v of a 20% albumin solution, e.g., a 20% human albumin solution.

[0379] In some embodiments, the culture medium comprises and/or is supplemented with from or from about 2 to or to about 6 g/L albumin, e.g., human albumin. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 2 to or to about 5.5, from or from about 2 to or to about 5.0, from or from about 2 to or to about 4.5, from or from about 2 to or to about 4, from or from about 2 to or to about 3.5, from or from about 2 to or to about 3, from or from about 2 to or to about 2.5, from or from about 2.5 to or to about 6, from or from about 2.5 to or to about 5.5, from or from about 2.5 to or to about 5.5, from or from about 2.5 to or to about 5.0, from or from about 2.5 to or to about 4.5, from or from about 2.5 to or to about 4.0, from or from about 2.5 to or to about 3.5, from or from about 2.5 to or to about 3.0, from or from about 3 to or to about 6, from or from about 3 to or to about 5.5, from or from about 3 to or to about 5, from or from about 3 to or to about 4.5, from or from about 3 to or to about 4, from or from about 3 to or to about 3.5, from or from about 3.5 to or to about 6, from or from about 3.5 to or to about 5.5, from or from about 3.5 to or to about 5, from or from about 3.5 to or to about 4.5, from or from about 3.5 to or to about 4, from or from about 4 to or to about 6, from or from about 4 to or to about 5.5, from or from about 4 to or to about 5, from or from about 4 to or to about 4.5, from or from about 4.5 to or to about 6, from or from about 4.5 to or to about 5.5, from or from about 4.5 to or to about 5, from or from about 5 to or to about 6, from or from about 5 to or to about 5.5, or from or from about 5.5 to or to about 6 g/L albumin, e.g., human albumin. In some embodiments, the culture medium comprises and/or is supplemented with from 3.2 to 4.8 g/L albumin, e.g., human albumin. In some embodiments, the culture medium comprises 4 g/L albumin, e.g., human albumin. In some embodiments, the culture medium comprises about 4 g/L albumin, e.g., human albumin

[0380] In some embodiments, the culture medium is supplemented with Poloxamer 188. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.1 to or to about 2.0 g/L Poloxamer 188. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 0.1 to or to about 1.8, from or from about 0.1 to or to about 1.6, from or from about 0.1 to or to about 1.4, from or from about 0.1 to or to about 1.2, from or from about 0.1 to or to about 1.0, from or from about 0.1 to or to about 0.8, from or from about 0.1 to or to about 0.6, from or from about 0. 1 to or to about 0.4, from or from about 0.1 to or to about 0.2, from or from about 0.2 to or to about 2.0, from or from about 0.2 to or to about 1.8, from or from about 0.2 to or to about 1.6, from or from about 0.2 to or to about 1.4, from or from about 0.2 to or to about 1.2, from or from about 0.2 to or to about 1.0, from or from about 0.2 to or to about 0.8, from or from about 0).2 to or to about 0.6, from or from about 0).2 to or to about 0.4, from or from about 0.4 to or to about 2.0, from or from about 0.4 to or to about 1.8, from or from about 0.4 to or to about 1.6, from or from about 0.4 to or to about 1.4, from or from about 0.4 to or to about 1.2, from or from about 0.4 to or to about 1.0, from or from about 0.4 to or to about 0.8, from or from about 0.4 to or to about 0.6, from or from about 0.6 to or to about 2.0, from or from about 0.6 to or to about 1.8, from or from about 0.6 to or to about 1.6, from or from about 0.6 to or to about 1.4, from or from about 0.6 to or to about 1.2, from or from about 0.6 to or to about 1.0, from or form about 0.6 to or to about 0.8, from or from about 0.8 to or to about 2.0, from or from about 0.8 to or to about 1.8, from or from about 0.8 to or to about 1.6, from or from about 0.8 to or to about 1.4, from or from about 0.8 to or to about 1.4, from or from about 0.8 to or to about 1.2, from or from about 0.8 to or to about 1.0, from or from about 1.0 to or to about 2.0, from or from about 1.0 to or to about 1.8, from or from about 1.0 to or to about 1.6, from or from about 1.0 to or to about 1.4, from or from about 1.0 to or to about 1.2, from or from about 1.2 to or to about 2.0, from or from about 1.2 to or to about 1.8, from or from about 1.2 to or to about 1.6, from or from about 1.2 to or to about 1.4, from or from about 1.4 to or to about 2.0, from or from about 1.4 to or to about 1.8, from or from about 1.4 to or to about 1.6, from or from about 1.6 to or to about 2.0, from or from about 1.6 to or to about 1.8, or from or from about 1.8 to or to about 2.0 g/L Poloxamer 188. In some embodiments, the culture medium comprises from 0.8 to 1.2 g/L Poloxamer 188. In some embodiments, the culture medium comprises 1.0 g/L Poloxamer 188. In some embodiments, the culture medium comprises about 1.0 g/L Poloxamer 188.

[0381] In some embodiments, the culture medium comprises and/or is supplemented with one or more antibiotics.

[0382] A first exemplary culture medium is set forth in Table 1.

TABLE-US-00001 TABLE 1 Exemplary Culture Medium #1 Exemplary Concentration Exemplary Component Range Concentration CellgroSCGM liquid medium undiluted undiluted Human Plasma 0.8-1.2% (v/v) 1.0% v/v Glutamine 3.2-4.8 mM 4.0 mM IL-2 64-96 g/L 80 g/L

[0383] A second exemplary culture medium is set forth in Table 2.

TABLE-US-00002 TABLE 2 Exemplary Culture Medium #2 Exemplary Concentration Exemplary Component Range Concentration RPMI1640 7.6-13.2 g/L 10.4 g/L Human Plasma 0.8-1.2% (v/v) 1.0% v/v Glucose 1.6-2.4 g/L 2.0 g/L Glutamine 3.2-4.8 mM 4.0 mM Sodium Pyruvate 0.8-1.2 mM 1.0 mM Sodium Hydrogen Carbonate 1.6-2.4 g/L 2.0 g/L IL-2 64-96 g/L 80 g/L Albumin 20% solution 1.6-2.5% v/v 2.0% v/v (3.2 to 4.8 g/L) (4.0 g/L) Poloxamer 188 0.8-1.2 g/L 1.0 g/L

CD3 Binding Antibodies

[0384] In some embodiments, the culture medium comprises and/or is supplemented with a CD3 binding antibody or antigen binding fragment thereof. In some embodiments, the CD3 binding antibody or antigen binding fragment thereof is selected from the group consisting of OKT3, UCHT1, and HIT3a, or variants thereof. In some embodiments, the CD3 binding antibody or antigen binding fragment thereof is OKT3 or an antigen binding fragment thereof.

[0385] In some embodiments, the CD3 binding antibody or antigen binding fragment thereof and feeder cells are added to the culture vessel before addition of NK cells and/or culture medium.

[0386] In some embodiments, the culture medium comprises and/or is supplemented with from or from about 5 ng/mL to or to about 15 ng/mL OKT3. In some embodiments, the culture medium comprises and/or is supplemented with from or from about 5 to or to about 12.5, from or from about 5 to or to about 10, from or from about 5 to or to about 7.5, from or from about 7.5 to or to about 15, from or from about 7.5 to or to about 12.5, from or from about 7.5 to or to about 10, from or from about 10 to or to about 15, from or from about 10 to or to about 12.5, or from or from about 12.5 to or to about 15 ng/mL OKT3. In some embodiments, the culture medium comprises and/or is supplemented with 10 ng/mL OKT3. In some embodiments, the culture medium comprises and/or is supplemented with about 10 ng/ml OKT3.

Culture Vessels

[0387] A number of vessels are consistent with the disclosure herein. In some embodiments, the culture vessel is selected from the group consisting of a flask, a bottle, a dish, a multiwall plate, a roller bottle, a bag, and a bioreactor.

[0388] In some embodiments, the culture vessel is treated to render it hydrophilic. In some embodiments, the culture vessel is treated to promote attachment and/or proliferation. In some embodiments, the culture vessel surface is coated with serum, collagen, laminin, gelatin, poy-L-lysine, fibronectin, extracellular matrix proteins, and combinations thereof.

[0389] In some embodiments, different types of culture vessels are used for different stages of culturing.

[0390] In some embodiments, the culture vessel has a volume of from or from about 100 mL to or to about 1,000 L. In some embodiments, the culture vessel has a volume of or about 125 mL, of or about 250 mL, of or about 500 mL, of or about 1 L, of or about 5 L, of about 10 L, or of or about 20 L.

[0391] In some embodiments, the culture vessel is a bioreactor.

[0392] In some embodiments, the bioreactor is a rocking bed (wave motion) bioreactor. In some embodiments, the bioreactor is a stirred tank bioreactor. In some embodiments, the bioreactor is a rotating wall vessel. In some embodiments, the bioreactor is a perfusion bioreactor. In some embodiments, the bioreactor is an isolation/expansion automated system. In some embodiments, the bioreactor is an automated or semi-automated bioreactor. In some embodiments, the bioreactor is a disposable bag bioreactor.

[0393] In some embodiments, the bioreactor has a volume of from about 100 mL to about 1,000 L. In some embodiments, the bioreactor has a volume of from about 10 L to about 1,000 L. In some embodiments, the bioreactor has a volume of from about 100 L to about 900 L. In some embodiments, the bioreactor has a volume of from about 10 L to about 800 L. In some embodiments, the bioreactor has a volume of from about 10 L to about 700 L, about 10 L to about 600 L, about 10 L to about 500 L, about 10 L to about 400 L, about 10 L to about 300 L, about 10 L to about 200 L, about 10 L to about 100 L, about 10 L to about 90 L, about 10 L to about 80 L, about 10 L to about 70 L, about 10 L to about 60 L, about 10 L to about 50 L, about 10 L to about 40 L, about 10 L to about 30 L, about 10 L to about 20 L, about 20 L to about 1,000 L, about 20 L to about 900 L, about 20 L to about 800 L, about 20 L to about 700 L, about 20 L to about 600 L, about 20 L to about 500 L, about 20 L to about 400 L, about 20 L to about 300 L, about 20 L to about 200 L, about 20 L to about 100 L, about 20 L to about 90 L, about 20 L to about 80 L, about 20 L to about 70 L, about 20 L to about 60 L, about 20 L to about 50 L, about 20 L to about 40 L, about 20 L to about 30 L, about 30 L to about 1,000 L, about 30 L to about 900 L, about 30 L to about 800 L, about 30 L to about 700 L, about 30 L to about 600 L. about 30 L to about 500 L, about 30 L to about 400 L, about 30 L to about 300 L, about 30 L to about 200 L, about 30 L to about 100 L, about 30 L to about 90 L, about 30 L to about 80 L, about 30 L to about 70 L, about 30 L to about 60 L, about 30 L to about 50 L, about 30 L to about 40 L, about 40 L to about 1,000 L, about 40 L to about 900 L, about 40 L to about 800 L, about 40 L to about 700 L, about 40 L to about 600 L, about 40 L to about 500 L, about 40 L to about 400 L, about 40 L to about 300 L, about 40 L to about 200 L, about 40 L to about 100 L, about 40 L to about 90 L, about 40 L to about 80 L, about 40 L to about 70 L, about 40 L to about 60 L, about 40 L to about 50 L, about 50 L to about 1.000 L, about 50 L to about 900 L, about 50 L to about 800 L, about 50 L to about 700 L, about 50 L to about 600 L, about 50 L to about 500 L, about 50 L to about 400 L, about 50 L to about 300 L, about 50 L to about 200 L. about 50 L to about 100 L, about 50 L to about 90 L, about 50 L to about 80 L, about 50 L to about 70 L, about 50 L to about 60 L, about 60 L to about 1.000 L, about 60 L to about 900 L, about 60 L to about 800 L, about 60 L to about 700 L, about 60 L to about 600 L, about 60 L to about 500 L, about 60 L to about 400 L, about 60 L to about 300 L, about 60 L to about 200 L, about 60 L to about 100L, about 60 L to about 90 L, about 60 L to about 80 L, about 60 L to about 70 L, about 70 L to about 1,000 L, about 70 L to about 900 L, about 70 L to about 800 L, about 70 L to about 700 L, about 70 L to about 600 L, about 70 L to about 500 L, about 70 L to about 400 L, about 70 L to about 300 L, about 70 L to about 200 L, about 70 L to about 100 L, about 70 L to about 90 L, about 70 L to about 80 L, about 80 L to about 1,000 L, about 80 L to about 900 L, about 80 L to about 800 L, about 80 L to about 700 L, about 80 L to about 600 L, about 80 L to about 500 L, about 80 L to about 400 L, about 80 L to about 300 L, about 80 L to about 200 L, about 80 L to about 100 L, about 80 L to about 90 L, about 90 L to about 1,000 L, about 90 L to about 900 L, about 90 L to about 800 L, about 90 L to about 700 L, about 90 L to about 600 L, about 90 L to about 500 L, about 90 L to about 400 L, about 90 L to about 300 L, about 90 L to about 200 L, about 90 L to about 100 L, about 100 L to about 1,000 L, about 100 L to about 900 L, about 100 L to about 800 L, about 100 L to about 700 L, about 100 L toa bout 600 L, about 100 L to about 500 L, about 100 L to about 400 L, about 100 L to about 300 L, about 100 L to about 200 L, about 200 L to about 1,000 L, about 200 L to about 900 L, about 200 L to about 800 L, about 200 L to about 700 L, about 200 L to about 600 L, about 200 L to about 500 L, about 200 L to about 400 L, about 200 L to about 300 L, about 300 L to about 1,000 L, about 300 L to about 900 L, about 300 L to about 800 L, about 300 L to about 700 L, about 300 L to about 600 L, about 300 L to about 500 L, about 300 L to about 400 L, about 400 L to about 1,000 L, about 400 L to about 900 L, about 400 L to about 800 L, about 400 L to about 700 L, about 400 L to about 600 L, about 400 L to about 500 L, about 500 L to about 1,000 L, about 500 L to about 900 L, about 500 L to about 800 L, about 500 L to about 700 L, about 500 L to about 600 L, about 600 L to about 1,000 L, about 600 L to about 900 L, about 600 L to about 800 L, about 600 L to about 700 L, about 700 L to about 1,000 L, about 700 L to about 900 L, about 700 L to about 800 L, about 800 L to about 1,000 L, about 800 L to about 900 L, or about 900 L to about 1,000 L. In some embodiments, the bioreactor has a volume of about 50 L.

[0394] In some embodiments, the bioreactor has a volume of from 100 mL to 1,000 L. In some embodiments, the bioreactor has a volume of from 10 L to 1.000 L. In some embodiments, the bioreactor has a volume of from 100 L to 900 L. In some embodiments, the bioreactor has a volume of from 10 L to 800 L. In some embodiments, the bioreactor has a volume of from 10 L to 700 L, 10 L to 600 L, 10 L to 500 L, 10 L to 400 L, 10 L to 300 L, 10 L to 200 L, 10 L to 100 L, 10 L to 90 L, 10 L to 80 L, 10 L to 70 L, 10 L to 60 L, 10 L to 50 L, 10 L to 40 L, 10 L to 30 L, 10 L to 20 L, 20 L to 1,000 L, 20 L to 900 L, 20 L to 800 L, 20 L to 700 L, 20 L to 600 L, 20 L to 500 L, 20 L to 400 L, 20 L to 300 L, 20 L to 200 L, 20 L to 100 L, 20 L to 90 L, 20 L to 80

[0395] L, 20 L to 70 L, 20 L to 60 L, 20 L to 50 L, 20 L to 40 L, 20 L to 30 L, 30 L to 1,000 L, 30 L to 900 L, 30 L to 800 L. 30 L to 700 L, 30 L to 600 L, 30 L to 500 L, 30 L to 400 L. 30 L to 300 L, 30 L to 200 L, 30 L to 100 L, 30 L to 90 L, 30 L to 80 L, 30 L to 70 L, 30 L to 60 L, 30 L to 50 L, 30 L to 40 L, 40 L to 1,000 L, 40 L to 900 L, 40 L to 800 L, 40 L to 700 L, 40 L to 600 L, 40 L to 500 L, 40 L to 400 L, 40 L to 300 L, 40 L to 200 L, 40 L to 100 L, 40 L to 90 L, 40 L to 80 L, 40 L to 70 L, 40 L to 60 L, 40 L to 50 L, 50 L to 1,000 L, 50 L to 900 L, 50 L to 800 L, 50 L to 700 L, 50 L to 600 L, 50 L to 500 L, 50 L to 400 L, 50 L to 300 L, 50 L to 200 L, 50 L to 100 L, 50 L to 90 L, 50 L to 80 L, 50 L to 70 L, 50 L to 60 L, 60 L to 1,000 L, 60 L to 900 L, 60 L to 800 L, 60 L to 700 L. 60 L to 600 L, 60 L to 500 L, 60 L to 400 L, 60 L to 300 L. 60 L to 200 L, 60 L to 100L, 60 L to 90 L, 60 L to 80 L, 60 L to 70 L, 70 L to 1,000 L, 70 L to 900 L, 70 L to 800 L, 70 L to 700 L. 70 L to 600 L, 70 L to 500 L, 70 L to 400 L, 70 L to 300 L. 70 L to 200 L, 70 L to 100 L, 70 L to 90 L, 70 L to 80 L, 80 L to 1,000 L, 80 L to 900 L, 80 L to 800 L, 80 L to 700 L, 80 L to 600 L. 80 L to 500 L, 80 L to 400 L, 80 L to 300 L, 80 L to 200 L, 80 L to 100 L, 80 L to 90 L, 90 L to 1,000 L, 90 L to 900 L, 90 L to 800 L, 90 L to 700 L, 90 L to 600 L, 90 L to 500 L, 90 L to 400 L, 90 L to 300 L, 90 L to 200 L, 90 L to 100 L, 100 L to 1,000 L, 100 L to 900 L. 100 L to 800 L. 100 L to 700 L, 100 L to 600 L. 100 L to 500 L. 100 L to 400 L. 100 L to 300 L, 100 L to 200 L, 200 L to 1,000 L, 200 L to 900 L, 200 L to 800 L, 200 L to 700 L, 200 L to 600 L, 200 L to 500 L, 200 L to 400 L, 200 L to 300 L, 300 L to 1,000 L, 300 L to 900 L, 300 L to 800 L, 300 L to 700 L, 300 L to 600 L, 300 L to 500 L, 300 L to 400 L, 400 L to 1,000 L, 400 L to 900 L, 400 L to 800 L, 400 L to 700 L, 400 L to 600 L, 400 L to 500 L, 500 L to 1,000 L, 500 L to 900 L, 500 L to 800 L, 500 L to 700 L, 500 L to 600 L, 600 L to 1,000 L, 600 L to 900 L, 600 L to 800 L, 600 L to 700 L, 700 L to 1,000 L, 700 L to 900 L, 700 L to 800 L, 800 L to 1,000 L, 800 L to 900 L, or 900 L to 1,000 L. In some embodiments, the bioreactor has a volume of 50 L.

Cell Expansion and Stimulation

[0396] In some embodiments, the natural killer cell source, e.g., single unit of cord blood, is co-cultured with feeder cells to produce expanded and stimulated NK cells.

[0397] In some embodiments, the co-culture is carried out in a culture medium described herein, e.g., exemplary culture medium #1 (Table 1) or exemplary culture medium #2 (Table 2).

[0398] In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises from or from about 110.sup.7 to or to about 110.sup.9 total nucleated cells prior to expansion. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises from or from about 110.sup.8 to or to about 1.510.sup.8 total nucleated cells prior to expansion. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises 110.sup.8 total nucleated cells prior to expansion. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises about 110.sup.8 total nucleated cells prior to expansion. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises 110.sup.9 total nucleated cells prior to expansion. In some embodiments, the natural killer cell source, e.g., single unit of cord blood, comprises about 110.sup.9 total nucleated cells prior to expansion.

[0399] In some embodiments, cells from the co-culture of the natural killer cell source, e.g., single unit of cord blood and feeder cells are harvested and frozen, e.g., in a cryopreservation composition described herein. In some embodiments, the frozen cells from the co-culture are an infusion-ready drug product. In some embodiments, the frozen cells from the co-culture are used as a master cell bank (MCB) from which to produce an infusion-ready drug product, e.g., through one or more additional co-culturing steps, as described herein. Thus, for example, a natural killer cell source can be expanded and stimulated as described herein to produce expanded and stimulated NK cells suitable for use in an infusion-ready drug product without generating any intermediate products. A natural killer cell source can also be expanded and stimulated as described herein to produce an intermediate product, e.g., a first master cell bank (MCB). The first MCB can be used to produce expanded and stimulated NK cells suitable for use in an infusion-ready drug product, or, alternatively, be used to produce another intermediate product, e.g., a second MCB. The second MCB can be used to produce expanded and stimulated NK cells suitable for an infusion-ready drug product, or alternatively, be used to produce another intermediate product, e.g., a third MCB, and so on.

[0400] In some embodiments, the ratio of feeder cells to cells of the natural killer cell source or MCB cells inoculated into the co-culture is from or from about 1:1 to or to about 4:1. In some embodiments, the ratio of feeder cells to cells of the natural killer cell source or MCB cells is from or from about 1:1 to or to about 3.5:1, from or from about 1:1 to or to about 3:1, from or from about 1:1 to or to about 2.5:1, from or from about 1.1 to or to about 2:1, from or from about 1:1 to or to about 1.5:1, from or from about 1.5:1 to or to about 4:1, from or from about 1.5:1 to or to about 3.5:1, from or from about 1.5:1 to or to about 3:1, from or from about 1.5:1 to or to about 2.5:1, from or from about 1.5:1 to or to about 2:1, from or from about 2:1 to or to about 4:1, from or from about 2:1 to or to about 3.5:1, from or from about 2:1 to or to about 3:1, from or from about 2:1 to or to about 2.5:1, from or from about 2.5:1 to or to about 4:1, from or from about 2.5:1 to or to about 3.5:1, from or from about 2.5:1 to or to about 3:1, from or from about 3:1 to or to about 4:1, from or from about 3:1 to or to about 3.5:1, or from or from about 3.5:1 to or to about 4:1. In some embodiments, the ratio of feeder cells to cells of the natural killer cell source or MCB inoculated into the co-culture is 2.5:1. In some embodiments, the ratio of feeder cells to cells of the natural killer cell source or MCB inoculated into the co-culture is about 2.5:1.

[0401] In some embodiments, the co-culture is carried out in a disposable culture bag, e.g., a IL disposable culture bag. In some embodiments, the co-culture is carried out in a bioreactor, e.g., a 50L bioreactor. In some embodiments, culture medium is added to the co-culture after the initial inoculation.

[0402] In some embodiments, the co-culture is carried out for 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20. 21, 22, 23, 24 or more days. In some embodiments, the co-culture is carried out for a maximum of 16 days.

[0403] In some embodiments, the co-culture is carried out at 37 C., or about 37 C.

[0404] In some embodiments, the co-culture is carried out at pH 7.9 or about pH 7.9.

[0405] In some embodiments, the co-culture is carried out at a dissolved oxygen (DO) level of 50% or more.

[0406] In some embodiments, exemplary culture medium #1 (Table 1) is used to produce a MCB and exemplary culture medium #2 (Table 2) is used to produce cells suitable for an infusion-ready drug product.

[0407] In some embodiments, the co-culture of the natural killer cell source, e.g., single unit of cord blood, with feeder cells yields from or from about 5010.sup.8 to or to about 5010.sup.12cells, e.g., MCB cells or infusion-ready drug product cells. In some embodiments, the expansion yields from or from about 5010.sup.8 to or to about 2510.sup.10, from or from about 1010.sup.8 to or to about 110.sup.10, from or from about 5010.sup.8 to or to about 7510.sup.9 , from or from about 5010.sup.8 to or to about 5010.sup.9, from or from about 5010.sup.8 to or to about 2510.sup.9, from or from about 5010.sup.8 to or to about 110.sup.9, from or from about 5010.sup.8 to or to about 7510.sup.8, from or from about 7510.sup.8 to or to about 5010.sup.10, from or from about 7510.sup.8 to or to about 2510.sup.10, from or from about 7510.sup.8 to or to about 110.sup.10, from or from about 7510.sup.8 to or to about 75109, from or from about 7510.sup.8 to or to about 5010.sup.9, from or from about 7510.sup.8 to or to about 2510.sup.9, from or from about 7510.sup.8 to or to about 110.sup.9, from or from about 110.sup.9 to or to about 5010.sup.10, from or from about 110.sup.9 to or to about 2510.sup.10, from or from about 1109 to or to about 110.sup.10, from or from about 110.sup.9 to or to about 7510.sup.9 , from or from about 110.sup.9 to or to about 5010.sup.9, from or from about 110.sup.9 to or to about 2510.sup.9, from or from about 2510.sup.9 to or to about 5010.sup.10, from or from about 2510.sup.9 to or to about 2510.sup.10, from or from about 2510.sup.9 to or to about 110.sup.10, from or from about 2510.sup.9 to or to about 7510.sup.9, from or from about 2510.sup.9 to or to about 5010.sup.9, from or from about 5010.sup.9 to or to about 5010.sup.10, from or from about 5010.sup.9 to or to about 2510.sup.10, from or from about 5010.sup.9 to or to about 110.sup.10, from or from about 5010.sup.9 to or to about 7510.sup.9, from or from about 7510.sup.9 to or to about 5010.sup.10, from or from about 7510.sup.9 to or to about 2510.sup.10, from or from about 7510.sup.9 to or to about 110.sup.10, from or from about 110.sup.10 to or to about 5010.sup.10, from or from about 110.sup.10 to or to about 2510.sup.10, or from or from about 251010 to or to about 5010.sup.10 cells, e.g., e.g., MCB cells or infusion-ready drug product cells.

[0408] In some embodiments, the expansion yields from or from about 60 to or to about 100 vials, each comprising from or from about 600 million to or to about 1 billion cells, e.g., MCB cells or infusion-ready drug product cells. In some embodiments, the expansion yields 80 or about 80 vials, each comprising or consisting of 800 million or about 800 million cells, e.g., MCB cells or infusion-ready drug product cells.

[0409] In some embodiments, the expansion yields from or from about a 100 to or to about a 500 fold increase in the number of cells, e.g., the number of MCB NK cells relative to the number of cells, e.g., NK cells, in the natural killer cell source. In some embodiments, the expansion yields from or from about a 100 to or to about a 500, from or from about a 100 to or to about a 400, from or from about a 100 to or to about a 300, from or from about a 100 to or to about a 200, from or from about a 200 to or to about a 450, from or from about a 200 to or to about a 400, from or from about a 100 to or to about a 350, from or from about a 200 to or to about a 300, from or from about a 200 to or to about a 250, from or from about a 250 to or to about a 500, from or from about a 250 to or to about a 450, from or from about a 200 to or to about a 400, from or from about a 250 to or to about a 350, from or from about a 250 to or to about a 300, from or from about a 300 to or to about a 500, from or from about a 300 to or to about a 450, from or from about a 300 to or to about a 400, from or from about a 300 to or to about a 350, from or from about a 350 to or to about a 500, from or from about a 350 to or to about a 450, from or from about a 350 to or to about a 400 fold increase in the number of cells, e.g., the number of MCB cells relative to the number of cells, e.g., NK cells, in the natural killer cell source.

[0410] In some embodiments, the expansion yields from or from about a 100 to or to about a 70,000 fold increase in the number of cells, e.g., the number of MCB NK cells relative to the number of cells, e.g., NK cells, in the natural killer cell source. In some embodiments, the expansion yields at least a 10,000 fold, e.g., 15,000 fold, 20,000 fold, 25,000 fold, 30,000 fold, 35,000 fold, 40,000 fold, 45,000 fold, 50,000 fold, 55,000 fold, 60,000 fold, 65,000 fold, or 70,000 fold increase in the number of cells, e.g., the number of MCB NK cells relative to the number of cells, e.g., NK cells, in the natural killer cell source.

[0411] In some embodiments, the co-culture of the MCB cells and feeder cells yields from or from about 500 million to or to about 1.5 billion cells, e.g., NK cells suitable for use in an MCB and/or in an infusion-ready drug product. In some embodiments, the co-culture of the MCB cells and feeder cells yields from or from about 500 million to or to about 1.5 billion, from or from about 500 million to or to about 1.25 billion, from or from about 500 million to or to about 1 billion, from or from about 500 million to or to about 750 million, from or from about 750 million to or to about 1.5 billion, from or from about 500 million to or to about 1.25 billion, from or from about 750 million to or to about 1 billion, from or from about 1 billion to or to about 1.5 billion, from or from about 1 billion to or to about 1.25 billion, or from or from about 1.25 billion to or to about 1.5 billion cells, e.g., NK cells suitable for use in an MCB and/or an infusion-ready drug product.

[0412] In some embodiments, the co-culture of the MCB cells and feeder cells yields from or from about 50 to or to about 150 vials of cells, e.g., infusion-ready drug product cells, each comprising from or from about 750 million to or to about 1.25 billion cells, e.g., NK cells suitable for use in an MCB and/or an infusion-ready drug product. In some embodiments, the co-culture of the MCB cells and feeder cells yields 100 or about 100 vials, each comprising or consisting of 1 billion or about 1 billion cells, e.g., NK cells suitable for use in an MCB and/or an infusion-ready drug product.

[0413] In some embodiments, the expansion yields from or from about a 100 to or to about a 500 fold increase in the number of cells, e.g., the number of NK cells suitable for use in an MCB and/or an infusion-ready drug product relative to the number of starting MCB cells. In some embodiments, the expansion yields from or from about a 100 to or to about a 500, from or from about a 100 to or to about a 400, from or from about a 100 to or to about a 300, from or from about a 100 to or to about a 200, from or from about a 200 to or to about a 450, from or from about a 200 to or to about a 400, from or from about a 100 to or to about a 350, from or from about a 200 to or to about a 300, from or from about a 200 to or to about a 250, from or from about a 250 to or to about a 500, from or from about a 250 to or to about a 450, from or from about a 200 to or to about a 400, from or from about a 250 to or to about a 350, from or from about a 250 to or to about a 300, from or from about a 300 to or to about a 500, from or from about a 300 to or to about a 450, from or from about a 300 to or to about a 400, from or from about a 300 to or to about a 350, from or from about a 350 to or to about a 500, from or from about a 350 to or to about a 450, from or from about a 350 to or to about a 400 fold increase in the number of cells, e.g., the number of NK cells suitable for use in an MCB and/or an infusion-ready drug product relative to the number of starting MCB cells.

[0414] In some embodiments, the expansion yields from or from about a 100 to or to about a 70,000 fold increase in the number of cells, e.g., the number of NK cells suitable for use in an MCB and/or an infusion-ready drug product relative to the number of starting MCB NK cells. In some embodiments, the expansion yields at least a 10,000 fold, e.g., 15,000 fold, 20,000 fold, 25,000 fold, 30,000 fold, 35,000 fold, 40,000 fold, 45,000 fold, 50,000 fold, 55,000 fold, 60,000 fold. 65.000 fold, or 70.000 fold increase in the number of cells, e.g., the number of NK cells suitable for use in an MCB and/or an infusion-ready drug product relative to the number of starting MCB NK cells.

[0415] In embodiments where the cells are engineered during expansion and stimulation, as described herein, not all of the expanded and stimulated cells will necessarily be engineered successfully, e.g., transduced successfully, e.g., transduced successfully with a vector comprising a heterologous protein, e.g., a heterologous protein comprising a CAR and/or IL-15 as described herein. Thus, the methods described herein can further comprise sorting engineered cells, e.g., engineered cells described herein, away from non-engineered cells.

[0416] In some embodiments, the engineered cells, e.g., transduced cells, are sorted from the non-engineered cells, e.g., the non-transduced cells using a reagent specific to an antigen of the engineered cells, e.g., an antibody that targets an antigen of the engineered cells but not the non-engineered cells. In some embodiments, the antigen of the engineered cells is a component of a CAR, e.g., a CAR described herein.

[0417] Systems for antigen-based cell separation of cells are available commercially, e.g., the CliniMACSR sorting system (Miltenyi Biotec).

[0418] In some embodiments, the engineered cells, e.g., transduced cells, are sorted from the non-engineered cells, e.g., the non-transduced cells using flow cytometry.

[0419] In some embodiments, the sorted engineered cells are used as an MCB. In some embodiments, the sorted engineered cells are used as a component in an infusion-ready drug product.

[0420] In some embodiments, the engineered cells, e.g., transduced cells, are sorted from the non-engineered cells, e.g., the non-transduced cells using a microfluidic cell sorting method. Microfluidic cell sorting methods are described, for example, in Dalili et al., A Review of Sorting, Separation and Isolation of Cells and Microbeads for Biomedical Applications: Microfluidic Approaches, Analyst 144:87 (2019).

[0421] In some embodiments, from or from about 1% to or to about 99% of the expanded and stimulated cells are engineered successfully, e.g., transduced successfully, e.g., transduced successfully with a vector comprising a heterologous protein, e.g., a heterologous protein comprising a CAR and/or IL-15 as described herein. In some embodiments, from or from about 1% to or to about 90%, from or from about 1% to or to about 80%, from or from about 1% to or to about 70%, from or from about 1% to or to about 60%, from or from about 1% to or to about 50%, from or from about 1% to or to about 40%, from or from about 1% to or to about 30%. from or from about 1% to or to about 20%, from or from about 1% to or to about 10%, from or from about 1% to or to about 5%, from or from about 5% to or to about 99%, from or from about 5% to or to about 90%, from or from about 5% to or to about 80%, from or from about 5% to or to about 70%, from or from about 5% to or to about 60%, from or from about 5% to or to about 50%, from or from about 5% to or to about 40%, from or from about 5% to or to about 30%, from or from about 5% to or to about 20%, from or from about 5% to or to about 10%, from or from about 10% to or to about 99%, from or from about 10% to or to about 90%, from or from about 10% to or to about 80%, from or from about 10% to or to about 70%, from or from about 10% to or to about 60%, from or from about 10% to or to about 50%, from or from about 10% to or to about 40%, from or from about 10% to or to about 30%, from or from about 10% to or to about 20%, from or from about 20% to or to about 99%, from or from about 20% to or to about 90%, from or from about 20% to or to about 80%, from or from about 20% to or to about 70%, from or from about 20% to or to about 60%, from or from about 20% to or to about 50%, from or from about 20% to or to about 40%, from or from about 20% to or to about 30%, from or from about 30% to or to about 99%, from or from about 30% to or to about 90%, from or from about 30% to or to about 80%, from or from about 30% to or to about 70%, from or from about 30% to or to about 60%, from or from about 30% to or to about 50%, from or from about 30% to or to about 40%, from or from about 40% to or to about 99%, from or from about 40% to or to about 90%, from or from about 40% to or to about 80%, from or from about 40% to or to about 70%, from or from about 40% to or to about 70%, from or from about 40% to or to about 60%, from or from about 40% to or to about 50%, from or from about 50% to or to about 99%, from or from about 50% to or to about 90%, from or from about 50% to or to about 80%, from or from about 50% to or to about 70%, from or from about 50% to or to about 60%, from or from about 60% to or to about 99%, from or from about 60% to or to about 90%, from or from about 60% to or to about 80%, from or from about 60% to or to about 70%, from or from about 70% to or to about 99%, from or from about 70% to or to about 90%, from or from about 70% to or to about 80%, from or from about 80% to or to about 99%, from or from about 80% to or to about 90%, or from or from about 90% to or to about 99% of the expanded and stimulated cells are engineered successfully, e.g., transduced successfully, e.g., transduced successfully with a vector comprising a heterologous protein, e.g., a heterologous protein comprising a CAR and/or IL-15 as described herein.

[0422] In some embodiments, frozen cells of a first or second MCB are thawed and cultured. In some embodiments, a single vial of frozen cells of the first or second MCB e.g., a single vial comprising 800 or about 800 million cells, e.g., first or second MCB cells, are thawed and cultured. In some embodiments, the frozen first or second MCB cells are cultured with additional feeder cells to produce cells suitable for use either as a second or third MCB or in an infusion-ready drug product. In some embodiments, the cells from the co-culture of the first or second MCB are harvested and frozen.

[0423] In some embodiments, the cells from the co-culture of the natural killer cell source, a first MCB, or a second MCB are harvested, and frozen in a cryopreservation composition, e.g., a cryopreservation composition described herein. In some embodiments, the cells are washed after harvesting. Thus, provided herein is a pharmaceutical composition comprising activated and stimulated NK cells, e.g., activated and stimulated NK cells produced by the methods described herein, e.g., harvested and washed activated and stimulated NK cells produced by the methods described herein and a cryopreservation composition, e.g., a cryopreservation composition described herein.

[0424] In some embodiments, the cells are mixed with a cryopreservation composition, e.g., as described herein, before freezing. In some embodiments, the cells are frozen in cryobags. In some embodiments, the cells are frozen in cryovials.

[0425] In some embodiments, the method further comprises isolating NK cells from the population of expanded and stimulated NK cells.

[0426] An exemplary process for expanding and stimulating NK cells is shown in FIG. 1.

Engineering

[0427] In some embodiments, the method further comprises engineering NK cell(s), e.g., to express a heterologous protein, e.g., a heterologous protein described herein, e.g., a heterologous protein comprising a CAR and/or IL-15.

[0428] In some embodiments, engineering the NK cell(s) to express a heterologous protein described herein comprises transforming, e.g., stably transforming the NK cells with a vector comprising a polynucleic acid encoding a heterologous protein described herein. Suitable vectors are described herein.

[0429] In some embodiments, engineering the NK cell(s) to express a heterologous protein described herein comprises introducing the heterologous protein via gene editing (e.g., zinc finger nuclease (ZFN) gene editing, ARCUS gene editing. CRISPR-Cas9 gene editing, or megaTAL gene editing) combined with adeno-associated virus (AAV) technology.

[0430] In some embodiments, the NK cell(s) are engineered to express a heterologous protein described herein, e.g., during or after culturing the composition in a medium comprising feeder cells. For example, in some cases, engineering (e.g., transduction) occurs during the expansion and stimulation process described herein, e.g., during co-culturing NK cell source(s) and feeder cell(s) as described herein, e.g., at day 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16, 17, or 18 of co-culture.

[0431] In some embodiments, the method further comprises engineering NK cell(s), e.g., to express, over-express, knock-out, or knock-down gene(s) or gene product(s).

[0432] In some embodiments, the natural killer cells are not genetically engineered.

[0433] In some embodiments, the NK cell(s) are engineered (e.g., transduced) in a culture medium supplemented with a stimulating factor (e.g., as described herein). Such cytokines can be used to provide growth or survival signals to the NK cells during the engineering process or to increase transduction efficiency. In some embodiments, the stimulation factor(s) are cytokine(s). In some embodiments, the cytokine(s) are selected from the group consisting of IL-2. IL-12, IL-15, IL-18, IL-21, IL-23, IL-27, IFN-, IFN, and combinations thereof.

[0434] In some embodiments, the cytokine is IL-21. IL-21 can be used at a final concentration of between 10 and 100 ng/mL, including, for example, at or at about 10, 15, 20, 25, 30, 34, 40, 45, 50, 55, 60, 70. 80, 90, or 100 ng/mL. In some embodiments, the cytokine is IL-2. In some embodiments, the cytokines are a combination of IL-2 and IL-21. In some embodiments, the cytokines are a combination of IL-2, IL-18, and IL-21.

[0435] In some embodiments, the stimulating factor is added to the culture medium at the time of engineering (e.g., transduction). In some embodiments, the stimulating factor is added to the culture medium after the time of engineered (e.g., transducing), e.g., from 1 to 48 hours after engineering, e.g., from 1 to 36, 1 to 24, 1 to 12, 12 to 28, 12 to 36, 12 to 24. 24 to 48, 24 to 36, or 36 to 48 hours after engineering. In some embodiments, the stimulating factor is added to the culture medium both at the time of transduction and after the time of engineering (e.g., from 1 to 48 hours after transduction).

[0436] In some embodiments, the culture is supplemented with the stimulating factor after culturing in a medium comprising feeder cells. Thus, in some cases, the culture medium will contain feeder cells at the time of engineering (e.g., transduction). In some cases, the feeder cells are removed from the culture prior to supplementation with the stimulating factor or engineering. In some cases, the feeder cells are not removed from the culture prior to supplementation with the stimulating factor or engineering. In some cases, no additional feeder cells are added to the culture during engineering, whether or not any residual feeder cells are removed. In some cases, both additional feeder cells and a stimulating factor are added to the culture during engineering. In some cases, additional feeder cells are not added to the culture during engineering but stimulating factors are added to the culture during engineering.

Properties of Expanded and Stimulated NK Cells

[0437] After having been ex vivo expanded and stimulated, e.g., as described herein, the expanded and stimulated NK cell populations not only have a number/density (e.g., as described above) that could not occur naturally in the human body, but they also differ in their phenotypic characteristics. (e.g., gene expression and/or surface protein expression) with the starting source material or other naturally occurring populations of NK cells.

[0438] In some cases, the starting NK cell source is a sample derived from a single individual, e.g., a single cord blood unit that has not been ex vivo expanded. Therefore, in some cases, the expanded and stimulated NK cells share a common lineage, i.e., they all result from expansion of the starting NK cell source, and, therefore, share a genotype via clonal expansion of a population of cells that are, themselves, from a single organism. Yet, they could not occur naturally at the density achieved with ex vivo expansion and also differ in phenotypic characteristics from the starting NK cell source.

[0439] In some cases, the population of expanded and stimulated NK cells comprises at least 100 million expanded natural killer cells, e.g., 200 million, 250 million, 300 million, 400 million, 500 million, 600 million, 700 million, 750 million, 800 million, 900 million, 1 billion, 2 billion, 3 billion, 4 billion, 5 billion, 6 billion, 7 billion, 8 billion, 9 billion, 10 billion, 15 billion, 20 billion, 25 billion, 50 billion, 75 billion, 80 billion, 9-billion, 100 billion, 200 billion, 250 billion, 300 billion, 400 billion, 500 billion, 600 billion, 700 billion, 800 billion, 900 billion, 1 trillion, 2 trillion, 3 trillion, 4 trillion, 5 trillion, 6 trillion, 7 trillion, 8 trillion, 9 trillion, or 10 trillion expanded natural killer cells.

[0440] In some embodiments, the expanded and stimulated NK cells comprise at least 80%, e.g., at least 90%, at least 95%, at least 99%, or 100% CD56+CD3-cells.

[0441] In some embodiments, the expanded and stimulated NK cells are not genetically engineered.

[0442] In some embodiments, the expanded and stimulated NK cells do not comprise a CD16 transgene.

[0443] In some embodiments, the expanded and stimulated NK cells do not express an exogenous CD16 protein.

[0444] The expanded and stimulated NK cells can be characterized, for example, by surface expression, e.g., of one or more of CD16, CD56, CD3, CD38, CD14, CD19, NKG2D, NKp46, NKp30. DNAM-1, and NKp44.

[0445] The surface protein expression levels stated herein, in some cases are achieved without positive selection on the particular surface protein referenced. For example, in some cases, the NK cell source, e.g., a single cord unit, comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and is + enriched and CD3 (+) depleted, e.g., by gating on CD56+CD3-expression. but no other surface protein expression selection is carried out during expansion and stimulation.

[0446] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKG2D+ cells.

[0447] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKp46+ cells.

[0448] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKp30+ cells.

[0449] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% DNAM-1+ cells.

[0450] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKp44+ cells. In some embodiments, the expanded and stimulated NK cells, e.g., from expansion

[0451] and stimulation of a single cord blood unit, e.g., as described above, comprise at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% CD94+ (KLRD1) cells.

[0452] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD3+ cells.

[0453] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD14+ cells.

[0454] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD19+ cells.

[0455] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CXCR+ cells.

[0456] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD122+ (IL2RB) cells.

[0457] As described herein, the inventors have demonstrated that, surprisingly, the NK cells expanded and stimulated by the methods described herein express CD16 at high levels throughout the expansion and stimulation process, resulting in a cell population with high CD16 expression. The high expression of CD16 obviates the need for engineering the expanded cells to express CD16, which is important for initiating ADCC, and, therefore, a surprising and unexpected benefit of the expansion and stimulation methods described herein. Thus, in some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise 50% or more, e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% CD16+NK cells.

[0458] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and comprise 50% or more, e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% CD16+NK cells.

[0459] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing CD16 is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0460] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing NKG2D is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0461] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing NKp30 is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0462] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing DNAM-1 is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0463] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing NKp44 is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0464] In some embodiments, the percentage of expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, expressing

[0465] NKp46 is the same or higher than the percentage of natural killer cells in the seed cells from umbilical cord blood.

[0466] As described herein, the inventors have also demonstrated that, surprisingly, the NK cells expanded and stimulated by the methods described herein express CD38 at low levels. CD38 is an effective target for certain cancer therapies (e.g., multiple myeloma and acute myeloid leukemia). See, e.g., . Jiao et al., CD38: Targeted Therapy in Multiple Myeloma and Therapeutic Potential for Solid Cancerrs, Expert Opinion on Investigational Drugs 29 (11): 1295-1308 (2020).

[0467] Thus, in some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprise less than or equal to 80% CD38+ cells, e.g., less than or equal to 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, or 20% CD38+ cells.

[0468] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and comprise less than or equal to 80% CD38+ cells, e.g., less than or equal to 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, or 20% CD38+ cells.

[0469] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and comprise less than or equal to 80% CD38+ cells, e.g., less than or equal to 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, or 20% CD38+ cells, and 50% or more, e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% CD16+NK cells.

[0470] In some embodiments, the expanded and stimulated NK cells, e.g., from expansion and stimulation of a single cord blood unit, e.g., as described above, comprises both the KIR B allele of the KIR receptor family and the 158 V/V variant of CD16 and comprise: i) 50% or more, e.g., 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or 95% CD16+NK cells; and/or ii) less than or equal to 80% CD38+ cells, e.g., less than or equal to 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, or 20% CD38+ cells; and/or iii) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKG2D+ cells; and/or iv) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100%

[0471] NKp46+ cells; and/or v) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKp30+ cells; and/or vi) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% DNAM-1+ cells; and/or vii) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% NKp44+ cells; and/or viii) at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% CD94+ (KLRD1) cells; and/or ix) less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD3+ cells; and/or x) less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD14+ cells; and/or xi) less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD19+ cells; and/or xii) less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CXCR+ cells; and/or xiii) less than or equal to 20%, e.g., less than or equal to 10%, less than or equal to 5%, less than or equal to 1% or 0% CD122+ (IL2RB) cells.

[0472] In some embodiments, feeder cells do not persist in the expanded and stimulated NK cells, though, residual signature of the feeder cells may be detected, for example, by the presence of residual cells (e.g., by detecting cells with a particular surface protein expression) or residual nucleic acid and/or proteins that are expressed by the feeder cells.

[0473] For example, in some cases, the methods described herein include expanding and stimulating natural killer cells using engineered feeder cells, e.g., eHuT-78 feeder cells described above, which are engineered to express sequences that are not expressed by cells in the natural killer cell source, including the natural killer cells. For example, the engineered feeder cells can be engineered to express at least one gene selected from the group consisting of 4-1BBL (UniProtKB P41273, SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and mutant TNFalpha (SEQ ID NO: 3) (eHut-78 cells), or variants thereof.

[0474] While these feeder cells may not persist in the expanded and stimulated NK cells, the expanded and stimulated NK cells may retain detectable residual amounts of cells, proteins, and/or nucleic acids from the feeder cells. Thus, their residual presence in the expanded and stimulated NK cells may be detected, for example by detecting the cells themselves (e.g., by flow cytometry), proteins that they express, and/or nucleic acids that they express.

[0475] Thus, also described herein is a population of expanded and stimulated NK cells comprising residual feeder cells (live cells or dead cells) or residual feeder cell cellular impurities (e.g., residual feeder cell proteins or portions thereof, and/or genetic material such as a nucleic acid or portion thereof). In some cases, the expanded and stimulated NK cells comprise more than 0) % and, but 0).3% or less residual feeder cells, e.g., eHuT-78 feeder cells.

[0476] In some cases, the expanded and stimulated NK cells comprise residual feeder cell nucleic acids, e.g., encoding residual 4-1BBL (UniProtKB P41273, SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and/or mutant TNFalpha (SEQ ID NO: 3) or portion(s) thereof. In some cases, the membrane bound IL-21 comprises a CD8 transmembrane domain

[0477] In some cases, the expanded and stimulated NK cells comprise a % residual feeder cells of more than 0% and less than or equal to 0.2%, as measured, e.g., by the relative proportion of a feeder cell specific protein or nucleic acid sequence (that is, a protein or nucleic acid sequence not expressed by the natural killer cells) in the sample. For example, by qPCR, e.g., as described herein.

[0478] In some embodiments, the residual feeder cells are CD4 (+) T cells. In some embodiments, the residual feeder cells are engineered CD4 (+) T cells. In some embodiments, the residual feeder cell cells are engineered to express at least one gene selected from the group consisting of 4-1BBL (UniProtKB P41273, SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and mutant TNFalpha (SEQ ID NO: 3) (el lut-78 cells), or variants thereof. Thus, in some cases, the feeder cell specific protein is 4-1BBL (UniProtKB P41273, SEQ ID NO: 1).

[0479] membrane bound IL-21 (SEQ ID NO: 2), and/or mutant TNFalpha (SEQ ID NO: 3). And, therefore, the feeder cell specific nucleic acid is a nucleic acid encoding 4-1BBL (UniProtKB P41273, SEQ ID NO: 1), membrane bound IL-21 (SEQ ID NO: 2), and/or mutant TNFalpha (SEQ ID NO: 3), or portion thereof. In some cases, the membrane bound IL-21 comprises a

[0480] CD8 transmembrane domain.

[0481] A wide variety of different methods can be used to analyze and detect the presence of nucleic acids or protein gene products in a biological sample. As used herein, detecting can refer to a method used to discover, determine, or confirm the existence or presence of a compound and/or substance (e.g., a cell, a protein and/or a nucleic acid). In some embodiments, a detecting method can be used to detect a protein. In some embodiments, detecting can include chemiluminescence or fluorescence techniques. In some embodiments, detecting can include immunological-based methods (e.g., quantitative enzyme-linked immunosorbent assay's (ELISA), Western blotting, or dot blotting) wherein antibodies are used to react specifically with entire proteins or specific epitopes of a protein. In some embodiments, detecting can include immunoprecipitation of the protein (Jungblut et al., J Biotechnol.31: 41 (2-3): 111-20 (1995); Franco et al., Eur.J Morphol. 39 (1): 3-25 (2001)). In some embodiments, a detecting method can be used to detect a nucleic acid (e.g., DNA and/or RNA). In some embodiments, detecting can include Northern blot analysis, nuclease protection assays (NPA), in situ hybridization, or reverse transcription-polymerase chain reaction (RT-PCR) (Raj et al., Nat. Methods 5, 877-879 (2008): Jin et al., J Clin Lab Anal. 11 (1): 2-9 (1997): Ahmed, J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 20 (2): 77-116 (2002)).

[0482] Thus, also described herein, are methods for detecting a population of expanded and stimulated NK cells, e.g., expanded and stimulated using the methods described herein, that have been co-cultured with engineered feeder cells, e.g., eHuT-78 feeder cells described herein.

Anti-Her2 Car-Nk

[0483] Provided herein are engineered cells, e.g., engineered natural killer cells, e.g., CAR-NK cells, e.g., anti-HER2 CAR-NK cells. In some embodiments, the CAR-NK cells are engineered to express IL-15.

[0484] In some embodiments, the natural killer cells are engineered, e.g., transduced, during expansion and stimulation, e.g., expansion and stimulation described herein. In some embodiments, the natural killer cells are engineered during expansion and stimulation, e.g., during production of a MCB, as described herein. In some embodiments, the natural killer cells are engineered during expansion and stimulation, e.g., during production of NK cells suitable for use in an injection-ready drug product and/or during production of a MCB, as described above. Thus, in some embodiments, the NK cell(s) are host cells and provided herein are NK host cell(s) expressing a heterogeneous protein, e.g., as described herein.

[0485] In some embodiments, the natural killer cells are engineered prior to expansion and stimulation. In some embodiments, the natural killer cells are engineered after expansion and stimulation.

[0486] In some embodiments, the NK cells are engineered by transducing with a vector. Suitable vectors are described herein, e.g., lentiviral vectors, e.g., a lentiviral vectors comprising a heterologous protein, e.g., as described herein. In some embodiments, the NK cells are transduced during production of a first MCB, as described herein.

[0487] In some embodiments, the NK cell(s) are transduced at a multiplicity of infection of from or from about 1 to or to about 40 viral particles per cell. In some embodiments, the NK cell(s) are transduced at a multiplicity of infection of or of about 1, of or of about 5, of or of about 10, of or of about 15, of or of about 20, of or of about 25, of or of about 30, of or of about 35, or of or of about 40 viral particles per cell.

Chimeric Antigen Receptors

[0488] In some embodiments, the heterologous protein is a fusion protein, e.g., a fusion protein comprising a chimeric antigen receptor (CAR) is introduced into the NK cell, e.g., during the expansion and stimulation process.

[0489] In some embodiments, the CAR comprises one or more of: a signal sequence, an extracellular domain, a hinge, a transmembrane domain, and one or more intracellular signaling domain sequences. In some embodiments, the CAR further comprises a spacer sequence.

[0490] In some embodiments, the CAR comprises (from N- to C-terminal): a signal sequence, an extracellular domain, a hinge, a spacer, a transmembrane domain, a first signaling domain sequence, a second signaling domain sequence, and a third signaling domain sequence.

[0491] In some embodiments, the CAR comprises (from N- to C-terminal): a signal sequence, an extracellular domain, a hinge, a transmembrane domain, a first signaling domain sequence, a second signaling domain sequence, and a third signaling domain sequence.

[0492] The signal sequence can be cleaved from a mature CAR protein. Such cleavage can be mediated by a signal peptidase and can occur either during or after completion of translocation to generate the mature protein. Thus, in some embodiments, the CAR comprises (from N- to C-terminal): an extracellular domain, a hinge, a spacer, a transmembrane domain, a first signaling domain sequence, a second signaling domain sequence, and a third signaling domain sequence.

[0493] In some embodiments, the CAR comprises (from N- to C-terminal): an extracellular domain, a hinge, a transmembrane domain, a first signaling domain sequence, a second signaling domain sequence, and a third signaling domain sequence.

[0494] In some embodiments the extracellular domain comprises an antibody or antigen-binding portion thereof.

[0495] In some embodiments, one or more of the intracellular signaling domain sequence(s) is a CD28 intracellular signaling sequence. In some embodiments, the CD28 intracellular signaling sequence comprises or consists of SEQ ID NO: 5.

[0496] In some embodiments, one or more of the intracellular signaling domain sequence(s) is an OX40L signaling sequence. Sec, e.g., . Matsumura et al., Intracellular Signaling of gp34, the OX40 Ligand: Induction of c-jun and c-fos mRNA Expression Through gp34 upon Binding of Its Receptor, OX40, J. Immunol 163:3007-11 (1999), which is hereby incorporated by reference in its entirety. In some embodiments, the OX40L signaling sequence comprises or consists of SEQ ID NO: 8. SEQ ID NO: 9, or SEQ ID NO: 10.

[0497] In some embodiments, one or more of the intracellular signaling sequence(s) is a CD3 {intracellular signaling domain sequence. In some embodiments, the CD3} intracellular signaling sequence comprises of consists of SEQ ID NO: 13.

[0498] In some embodiments, the CAR comprises a CD28 intracellular signaling sequence (SEQ ID NO: 5), an OX40L intracellular signaling sequence (SEQ ID NO: 8, SEQ ID NO: 9, or SEQ ID NO: 10), and a CD3} intracellular signaling sequence (SEQ ID NO: 13).

[0499] In some embodiments, the CAR comprises an intracellular signaling domain comprising or consisting of SEQ ID NO: 25.

[0500] In some embodiments, the CAR does not comprise an OX40L intracellular signaling domain sequence.

[0501] In some embodiments, the CAR comprises a CD28 intracellular signaling sequence (SEQ ID NO: 5), and a CD35 intracellular signaling sequence (SEQ ID NO: 13), but not an OX40L intracellular signaling domain sequence.

[0502] In some embodiments, the signal sequence is a CD8a signal sequence. In some embodiments, the signal sequence comprises or consists of SEQ ID NO: 27.

[0503] In some embodiments, the extracellular domain comprises a single-chain variable fragment (scFv). In some embodiments, the extracellular domain comprises an anti-HER2 antibody or antigen binding fragment thereof. In some embodiments, the extracellular domain comprises an anti-HER2 scFv.

[0504] In some embodiments, the anti-HER2 scFv comprises a CDRL1 domain comprising or consisting of SEQ ID NO: 34, a CDRL2 domain comprising or consisting of SEQ ID NO: 36, a CDRL3 domain comprising or consisting of SEQ ID NO: 38, a CDRH1 domain comprising or consisting of SEQ ID NO: 44, a CDRH2 domain comprising or consisting of SEQ ID NO: 46, and a CDRH3 domain comprising or consisting of SEQ ID NO: 48.

[0505] In some embodiments, the anti-HER2 scFv comprises a VL domain comprising or consisting of SEQ ID NO: 32 and a V.sub.H domain comprising or consisting of SEQ ID NO: 42.

[0506] In some embodiments, the anti-HER2 scFv comprises a VL domain comprising or consisting of SEQ ID NO: 32, a linker comprising or consisting of SEQ ID NO: 40, and a V.sub.H domain comprising or consisting of SEQ ID NO: 42.

[0507] In some embodiments, the anti-HER2 scFv comprises or consists of SEQ ID NO: 30.

[0508] In some embodiments, the hinge comprises or consists of a CD8a hinge. In some embodiments, the CD8a hinge comprises or consists of SEQ ID NO: 50.

[0509] In some embodiments, the transmembrane domain is a CD28 transmembrane domain.

[0510] In some embodiments, the CD28 transmembrane domain comprises of consists of SEQ ID NO: 53.

[0511] In some embodiments, the fusion protein comprises or consists of SEQ ID NO: 56. IL-15

[0512] In some embodiments, the NK cell is engineered to express IL-15, e.g., human IL-15 (UniProtKB #P40933: NCBI Gene ID #3600), e.g., soluble human IL-15 or an ortholog thereof, or a variant of any of the foregoing. In some embodiments, the IL-15 is expressed as part of a fusion protein further comprising a cleavage site. In some embodiments, the IL-15 is expressed as part of a polyprotein comprising a self-cleaving peptide such as a T2A ribosomal skip sequence site (sometimes referred to as a self-cleaving site). See, e.g., . Radcliffe & Mitrophanous. Multiple Gene Products from a Single Vector: Self-Cleaving 2A Peptides.

[0513] Gene Therapy 11:1673-4 (2004); see also Liu et al., Systematic Comparison of 2A Peptides for Cloning Multi-Genes in a Polycistronic Vector, Scientific Reports 7 (1): 2193 (2017). In some embodiments, the IL-15 comprises or consists of SEQ ID NO: 22.

[0514] In some embodiments, the self-cleaving peptide is a 2A self-cleaving peptide. In some embodiments, the self-cleaving peptide is a T2A. P2A. E2A, or F2A self-cleaving peptide. In some embodiments, the self-cleaving peptide comprises SEQ ID NO: 16. In some embodiments, the self-cleaving peptide comprises or consists of SEQ ID NO: 16, SEQ ID NO:

[0515] 17, SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21.

[0516] In some embodiments, the T2A cleavage site comprises or consists of SEQ ID NO: 17.

[0517] In some embodiments, the IL-15 is expressed as part of a fusion protein comprising a CAR, e.g., a CAR described herein.

[0518] In some embodiments, the fusion protein comprises (oriented from N-terminally to C-terminally): a CAR comprising, a cleavage site, and IL-15.

[0519] In some embodiments, the fusion protein comprises SEQ ID NO: 26.

[0520] In some embodiment, the fusion protein comprises or consists of SEQ ID NO: 59.

Inhibitory Receptors

[0521] In some embodiments, the NK cell is engineered to alter, e.g., reduce, expression of one or more inhibitor receptor genes.

[0522] In some embodiments, the inhibitory receptor gene is a HLA-specific inhibitory receptor. In some embodiments, the inhibitory receptor gene is a non-HLA-specific inhibitory receptor.

[0523] In some embodiments, the inhibitor receptor gene is selected from the group consisting of KIR, CD94/NKG2A, LILRBI, PD-1, Irp60, Siglec-7, LAIR-1, and combinations thereof.

Polynucleic Acids, Vectors, and Host Cells

[0524] Also provided herein are polynucleic acids encoding the fusion protein(s) or portions thereof, e.g., the polynucleotide sequences encoding the polypeptides described herein, as shown in the Table of sequences provided herein

[0525] Also provided herein are vector(s) comprising the polynucleic acids, and cells, e.g., NK cells, comprising the vector(s).

[0526] In some embodiments, the vector is a lentivirus vector. See, e.g., . Milone et al.,

[0527] Clinical Use of Lentiviral Vectors, Leukemia 32:1529-41 (2018). In some embodiments, the vector is a retrovirus vector. In some embodiments, the vector is a gamma retroviral vector. In some embodiments, the vector is a non-viral vector, e.g., a piggy back non-viral vector (PB transposon, see, e.g., . Wu et al . . . piggyback is a Flexible and Highly Active Transposon as Compared to Sleeping Beauty, Tol2, and Mosl in Mammalian Cells, PNAS 103 (41): 15008-13 (2006)), a sleeping beauty non-viral vector (SB transposon, see, e.g., Hudecek et al., Going Non-Viral: the Sleeping Beauty Transposon System Breaks on Through to the Clinical Side, Critical Reviews in Biochemistry and Molecular Biology 52 (4): 355-380 (2017)), or an mRNA vector.

Cryopreservation

Cryopreservation Compositions

[0528] Provided herein are cryopreservation compositions, e.g., cryopreservation compositions suitable for intravenous administration, e.g., intravenous administration of NK cells, e.g., the NK cells described herein. In some embodiments, a pharmaceutical composition comprises the cryopreservation composition and cells, e.g., the NK cells described herein.

Albumin

[0529] In some embodiments, the cryopreservation composition comprises albumin protein, e.g., human albumin protein (UniProtKB Accession P0278, SEQ ID NO: 63) or variant thereof. In some embodiments, the cryopreservation composition comprises an ortholog of an albumin protein, e.g., human albumin protein, or variant thereof. In some embodiments, the cryopreservation composition comprises a biologically active portion of an albumin protein, e.g., human albumin, or variant thereof.

[0530] In some embodiments, the albumin, e.g., human albumin, is provided as a solution, also referred to herein as an albumin solution or a human albumin solution. Thus, in some embodiments, the cryopreservation composition is or comprises an albumin solution, e.g., a human albumin solution. In some embodiments, the albumin solution is a serum-free albumin solution.

[0531] In some embodiments, the albumin solution is suitable for intravenous use.

[0532] In some embodiments, the albumin solution comprises from or from about 40 to or to about 200 g/L albumin. In some embodiments, the albumin solution comprises from or from about 40 to or to about 50 g/L albumin, e.g., human albumin. In some embodiments, the albumin solution comprises about 200 g/L albumin, e.g., human albumin. In some embodiments, the albumin solution comprises 200 g/L albumin, e.g., human albumin.

[0533] In some embodiments, the albumin solution comprises a protein composition, of which 95% or more is albumin protein, e.g., human albumin protein. In some embodiments. 96%, 97%, 98%, or 99% or more of the protein is albumin, e.g., human albumin.

[0534] In some embodiments, the albumin solution further comprises sodium. In some embodiments, the albumin solution comprises from or from about 100 to or to about 200 mmol sodium. In some embodiments, the albumin solution comprises from or from about 130 to or to about 160 mmol sodium.

[0535] In some embodiments, the albumin solution further comprises potassium. In some embodiments, the albumin solution comprises 3 mmol or less potassium. In some embodiments, the albumin solution further comprises 2 mmol or less potassium.

[0536] In some embodiments, the albumin solution further comprises one or more stabilizers. In some embodiments, the stabilizer(s) are selected from the group consisting of sodium caprylate, caprylic acid, (2S)-2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as acetyl tryptophan, N-Acetyl-L-tryptophan and Acetyl-L-tryptophan), 2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as N-acetyltryptophan, DL-Acetyltroptohan and N-Acetyl-DL-tryptophan). In some embodiments, the solution comprises less than. 1 mmol of each of the one or more stabilizers per gram of protein in the solution. In some embodiments, the solution comprises from or from about 0.05 to or to about 0.1, e.g., from or from about 0.064 to or to about 0.096 mmol of each of the stabilizers per gram of protein in the solution. In some embodiments, the solution comprises less than 0.1 mmol of total stabilizer per gram of protein in the solution. In some embodiments, the solution comprises from or from about 0.05 to or to about 0.1, e.g., from or from about 0.064 to or to about 0.096 mmol of total stabilizer per gram of protein in the solution.

[0537] In some embodiments, the albumin solution consists of a protein composition, of which 95% or more is albumin protein, sodium, potassium, and one or more stabilizers selected from the group consisting of sodium caprylate, caprylic acid, (2S)-2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as acetyl tryptophan, N-Acetyl-L-tryptophan and Acetyl-L-tryptophan), 2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as N-acetyltryptophan, DL-Acetyltroptohan and N-Acetyl-DL-tryptophan) in water.

[0538] In some embodiments, the cryopreservation composition comprises from or from about 10% v/v to or to about 50% v/v of an albumin solution, e.g., an albumin solution described herein. In some embodiments, the cryopreservation composition comprises from or from about 10% to or to about 50%, from or from about 10% to or to about 45%, from or from about 10% to or to about 40%, from or from about 10% to or to about 35%, from or from about 10% to or to about 30%, from or from about 10% to or to about 25%, from or from about 10% to or to about 20%, from or from about 10% to or to about 15%, from or from about 15% to or to about 50%, from or from about 15% to or to about 45%, from or from about 15% to or to about 40%, from or from about 15% to or to about 35%, from or from about 15% to or to about 30%, from or from about 15% to or to about 25%, from or from about 15% to or to about 20%, from or from about 20% to or to about 50%, from or from about 20% to or to about 45%, from or from about 20% to or to about 40%, from or from about 20% to or to about 35%, from or from about 20% to or to about 30%, from or from about 20% to or to about 25%, from or from about 25% to or to about 50%, from or from about 25% to or to about 45%, from or from about 25% to or to about 40%, from or from about 25% to or to about 35%, from or from about 25% to or to about 30%, from or from about 30% to or to about 50%, from or from about 30% to or to about 45%, from or from about 30% to or to about 40%, from or from about 30% to or to about 35%, from or from about 35% to or to about 50%, from or from about 35% to or to about 45%, from or from about 35% to or to about 40%, from or from about 40% to or to about 50%, from or from about 40% to or to about 45%, or from or from about 45% to or to about 50% v/v of an albumin solution described herein. In some embodiments, the cryopreservation composition comprises about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, or about 50% v/v of an albumin solution described herein. In some embodiments, the cryopreservation composition comprises 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% v/v of an albumin solution described herein.

[0539] In some embodiments, the cryopreservation composition comprises from or from about 20 to or to about 100 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises from or from about 20 to or to about 100, from or from about 20 to or to about 90, from or from about 20 to or to about 80, from or from about 20 to or to about 70, from or from about 20 to or to about 60, from or from about 20 to or to about 50, from or from about 20 to or to about 40, from or from about 20 to or to about 30, from or from about 30 to or to about 100, from or from about 30 to or to about 90, from or from about 30 to or to about 80, from or from about 30 to or to about 70, from or from about 30 to or to about 60, from or from about 30 to or to about 50, from or from about 30 to or to about 40, from or from about 40 to or to about 100, from or from about 40 to or to about 90, from or from about 40 to or to about 80, from or from about 40 to or to about 70, from or from about 40 to or to about 60, from or from about 40 to or to about 50, from or from about 50 to or to about 100, from or from about 50 to or to about 90, from or from about 50 to or to about 80, from or from about 50 to or to about 70, from or from about 50 to or to about 60, from or from about 60 to or to about 100, from or from about 60 to or to about 90, from or from about 60 to or to about 80, from or from about 60 to or to about 70, from or from about 70 to or to about 100, from or from about 70 to or to about 90, from or from about 70 to or to about 80, from or from about 80 to or to about 100, from or from about 80 to or to about 90, or from or from about 90 to or to about 100 g/L albumin, e.g., human albumin.

[0540] In some embodiments, the cryopreservation composition comprises 20 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises 40 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises 70 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises 100 g/L albumin, e.g., human albumin.

[0541] In some embodiments, the cryopreservation composition comprises about 20 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises about 40 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises about 70 g/L albumin, e.g., human albumin. In some embodiments, the cryopreservation composition comprises about 100 g/L albumin, e.g., human albumin.

[0542] In some embodiments, the cryopreservation composition further comprises a stabilizer, e.g., an albumin stabilizer. In some embodiments, the stabilizer(s) are selected from the group consisting of sodium caprylate, caprylic acid, (2S)-2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as acetyl tryptophan. N-Acetyl-L-tryptophan and Acetyl-L-tryptophan), 2-acetamido-3-(1H-indol-3-yl) propanoic acid (also referred to as N-acetyltryptophan, DL-Acetyltroptohan and N-Acetyl-DL-tryptophan). In some embodiments, the cryopreservation composition comprises less than. 1 mmol of each of the one or more stabilizers per gram of protein, e.g., per gram of albumin protein, in the composition. In some embodiments, the cryopreservation composition comprises from or from about 0.05 to or to about 0.1, e.g., from or from about 0.064 to or to about 0.096 mmol of each of the stabilizers per gram of protein, e.g., per gram of albumin protein in the composition. In some embodiments, the cryopreservation composition comprises less than 0.1 mmol of total stabilizer per gram of protein, e.g., per gram of albumin protein in the cryopreservation composition. In some embodiments, the cryopreservation composition comprises from or from about 0.05 to or to about 0.1, e.g., from or from about 0.064 to or to about 0.096 mmol of total stabilizer per gram of protein, e.g., per gram of albumin protein, in the cryopreservation composition.

Dextran

[0543] In some embodiments, the cryopreservation composition comprises Dextran, or a derivative thereof.

[0544] Dextran is a polymer of anhydroglucose composed of approximately 95% a-D-(1-6) linkages (designated (C6H1005) n). Dextran fractions are supplied in molecular weights of from about 1,000 Daltons to about 2,000,000 Daltons. They are designated by number (Dextran X), e.g., Dextran 1, Dextran 10, Dextran 40, Dextran 70, and so on, where X corresponds to the mean molecular weight divided by 1,000 Daltons. So, for example, Dextran 40 has an average molecular weight of or about 40,000 Daltons.

[0545] In some embodiments, the average molecular weight of the dextran is from or from about 1,000 Daltons to or to about 2,000,000 Daltons. In some embodiments, the average molecular weight of the dextran is or is about 40,000 Daltons. In some embodiments, the average molecular weight of the dextran is or is about 70,000 Daltons.

[0546] In some embodiments, the dextran is selected from the group consisting of Dextran

[0547] 40, Dextran 70, and combinations thereof. In some embodiments, the dextran is Dextran 40.

[0548] In some embodiments, the dextran, e.g., Dextran 40, is provided as a solution, also referred to herein as a dextran solution or a Dextran 40 solution. Thus, in some embodiments, the composition comprises a dextran solution, e.g., a Dextran 40 solution.

[0549] In some embodiments, the dextran solution is suitable for intravenous use.

[0550] In some embodiments, the dextran solution comprises about 5% to about 50% w/w

[0551] dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises from or from about 5% to or to about 50%, from or from about 5% to or to about 45%, from or from about 5% to or to about 40%, from or from about 5% to or to about 35%, from or from about 5% to or to about 30%, from or from about 5% to or to about 25%, from or from about 5% to or to about 20%, from or from about 5% to or to about 15%, from or from about 5% to or to about 10%.

[0552] from or from about 10% to or to about 50%, from or from about 10% to or to about 45%, from or from about 10% to or to about 40%, from or from about 10% to or to about 35%, from or from about 10% to or to about 30%, from or from about 10% to or to about 25%, from or from about 10% to or to about 20%, from or from about 10% to or to about 15%, from or from about 15% to or to about 50%, from or from about 15% to or to about 45%, from or from about 15% to or to about 40%, from or from about 15% to or to about 35%, from or from about 15% to or to about 30%, from or from about 15% to or to about 25%, from or from about 15% to or to about 20%, from or from about 20% to or to about 50%, from or from about 20% to or to about 45%, from or from about 20% to or to about 40%, from or from about 20% to or to about 35%, from or from about 20% to or to about 30%, from or from about 20% to or to about 25%, from or from about 25% to or to about 50%, from or from about 25% to or to about 45%, from or from about 25% to or to about 40%, from or from about 25% to or to about 35%, from or from about 25% to or to about 30%, from or from about 30% to or to about 50%, from or from about 30% to or to about 45%, from or from about 30% to or to about 40%, from or from about 30% to or to about 35%, from or from about 35% to or to about 50%, from or from about 35% to or to about 45%, from or from about 35% to or to about 40%, from or from about 40% to or to about 50%, from or from about 40% to or to about 45%, or from or from about 45% to or to about 50% w/w dextran, e.g.,

[0553] Dextran 40. In some embodiments, the dextran solution comprises 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% w/w dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, or about 50% w/w dextran, e.g., Dextran 40.

[0554] In some embodiments, the dextran solution comprises from or from about 25 g/L to or to about 200 g/L dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises from or from about 35 to or to about 200, from or from about 25 to or to about 175, from or from about 25 to or to about 150, from or from about 25 to or to about 125, from or from about 25 to or to about 100, from or from about 25 to or to about 75, from or from about 25 to or to about 50, from or from about 50 to or to about 200, from or from about 50 to or to about 175. from or from about 50 to or to about 150, from or from about 50 to or to about 125, from or from about 50 to or to about 100, from or from about 50 to or to about 75, from or from about 75 to or to about 200, from or from about 75 to or to about 175, from or from about 75 to or to about 150, from or from about 75 to or to about 125, from or from about 75 to or to about 100, from or from about 100 to or to about 200, from or from about 100 to or to about 175, from or from about 100 to or to about 150, from or from about 100 to or to about 125, from or from about 125 to or to about 200, from or from about 125 to or to about 175, from or from about 125 to or to about 150, from or from about 150 to or to about 200, from or from about 150 to or to about 175, or from or from about 175 to or to about 200 g/L dextran e.g., Dextran 40. In some embodiments, the dextran solution comprises 25, 50, 75, 100, 125, 150, 175, or 200 g/L dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises 100 g/L dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises about 25, about 50, about 75, about 100, about 125, about 150, about 175, or about 200 g/L dextran, e.g., Dextran 40. In some embodiments, the dextran solution comprises about 100 g/L dextran, e.g., Dextran 40.

[0555] In some embodiments, the dextran solution further comprises glucose (also referred to as dextrose). In some embodiments, the dextran solution comprises from or from about 10 g/L to or to about 100 g/L glucose. In some embodiments, the dextran solution comprises from or from about 10 to or to about 100, from or from about 10 to or to about 90, from or from about 10 to or to about 80, from or from about 10 to or to about 70, from or from about 10 to or to about 60, from or from about 10 to or to about 50, from or from about 10 to or to about 40, from or from about 10 to or to about 30, from or from about 10 to or to about 20, from or from about 20 to or to about 100, from or from about 20 to or to about 90, from or from about 20 to or to about 80, from or from about 20 to or to about 70, from or from about 20 to or to about 60, from or from about 20 to or to about 50, from or from about 20 to or to about 40, from or from about 20 to or to about 30, from or from about 30 to or to about 100, from or from about 30 to or to about 90, from or from about 30 to or to about 80, from or from about 30 to or to about 70, from or from about 30 to or to about 60, from or from about 30 to or to about 50, from or from about 30 to or to about 40, from or from about 40 to or to about 100, from or from about 40 to or to about 90, from or from about 40 to or to about 80, from or from about 40 to or to about 70, from or from about 40 to or to about 60, from or from about 40 to or to about 50, from or from about 50 to or to about 100, from or from about 50 to or to about 90, from or from about 50 to or to about 80, from or from about 50 to or to about 70, from or from about 50 to or to about 60, from or from about 60 to or to about 100, from or from about 60 to or to about 90, from or from about 60 to or to about 80, from or from about 60 to or to about 70, from or from about 70 to or to about 100, from or from about 70 to or to about 90, from or from about 70 to or to about 80, from or from about 80 to or to about 90, from or from about 80 to or to about 100, from or from about 80 to or to about 90, or from or from about 90 to or to about 100 g/L glucose. In some embodiments, the dextran solution comprises 10, 20, 30, 40. 50. 60, 70, 80, 90, or 100 g/L glucose. In some embodiments, the dextran solution comprises 50 g/L glucose. In some embodiments, the dextran solution comprises about 10, about 20, about 30, about 40, about 50, about 60, about 70, about 80, about 90, or about 100 g/L glucose. In some embodiments, the dextran solution comprises 50 g/L glucose.

[0556] In some embodiments, the dextran solution consists of dextran, e.g., Dextran 40, and glucose in water.

[0557] In some embodiments, the cryopreservation composition comprises from or from about 10% v/v to or to about 50% v/v of a dextran solution described herein. In some embodiments, the cryopreservation composition comprises from or from about 10% to 50%, from or from about 10% to or to about 45%, from or from about 10% to or to about 40%, from or from about 10% to or to about 35%, from or from about 10% to or to about 30%, from or from about 10% to or to about 25%, from or from about 10% to or to about 20%, from or from about 10% to or to about 15%, from or from about 15% to or to about 50%, from or from about 15% to or to about 45%, from or from about 15% to or to about 40%, from or from about 15% to or to about 35%, from or from about 15% to or to about 30%, from or from about 15% to or to about 25%, from or from about 15% to or to about 20%, from or from about 20% to or to about 50%, from or from about 20% to or to about 45%, from or from about 20% to or to about 40%, from or from about 20% to or to about 35%, from or from about 20% to or to about 30%, from or from about 20% to or to about 25%, from or from about 25% to or to about 50%, from or from about 25% to or to about 45%, from or from about 25% to or to about 40%, from or from about 25% to or to about 35%, from or from about 25% to or to about 30%, from or from about 30% to or to about 50%, from or from about 30% to or to about 45%, from or from about 30% to or to about 40%, from or from about 30% to or to about 35%, from or from about 35% to or to about 50%, from or from about 35% to or to about 45%, from or from about 35% to or to about 40%, from or from about 40% to or to about 50%, from or from about 40% to or to about 45%, or from or from about 45% to or to about 50% v/v of a dextran solution, e.g., a dextran solution described herein. In some embodiments, the cryopreservation composition comprises 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, or 50% v/v of a dextran solution, e.g., a dextran solution described herein. In some embodiments, the cryopreservation composition comprises about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, or about 50% v/v of a dextran solution, e.g., a dextran solution described herein.

[0558] In some embodiments, the cryopreservation composition comprises from or from about 10 to or to about 50 g/L dextran, e.g., Dextran 40. In some embodiments, the cryopreservation composition comprises from or from about 10 to or to about 50, from or from about 10 to or to about 45, from or from about 10 to or to about 40, from or from about 10 to or to about 35, from or from about 10 to or to about 30, from or from about 10 to or to about 25, from or from about 10 to or to about 20, from or from about 10 to or to about 15, from or from about 15 to or to about 50, from or from about 15 to or to about 45, from or from about 15 to or to about 40, from or from about 15 to or to about 35, from or from about 15 to or to about 30, from or from about 15 to or to about 25, from or from about 15 to or to about 20, from or from about 20 to or to about 50, from or from about 20 to or to about 45, from or from about 20 to or to about 40, from or from about 20 to or to about 30, from or from about 20 to or to about 25, from or from about 25 to or to about 50, from or from about 25 to or to about 45, from or from about 25 to or to about 40, from or from about 25 to or to about 35, from or from about 25 to or to about 30, from or from about 30 to or to about 50, from or from about 30 to or to about 45, from or from about 30 to or to about 40, from or from about 30 to or to about 35, from or from about 35 to or to about 50, from or from about 35 to or to about 45, from or from about 35 to or to about 40, from or from about 40 to or to about 50, from or from about 40 to or to about 45, or from or from about 45 to or to about 50 g/L dextran, e.g., Dextran 40. In some embodiments, the cryopreservation composition comprises 10, 15, 20, 25, 30, 30, 35, 40, 45, or 50 g/L dextran, e.g., Dextran 40. In some embodiments, the cryopreservation composition comprises about 10, about 15, about 20, about 25, about 30, about 30, about 35, about 40, about 45, or about 50 g/L dextran, e.g., Dextran 40.

Glucose

[0559] In some embodiments, the cryopreservation composition comprises glucose (D-glucose and/or L-glucose).

[0560] In some embodiments, as described above, the cryopreservation composition comprises a Dextran solution comprising glucose.

[0561] In some embodiments, the cryopreservation composition comprises a Dextran solution that does not comprise glucose. In some embodiments, e.g., when the Dextran solution does not comprise glucose, glucose is added separately to the cryopreservation composition.

[0562] In some embodiments, the cryopreservation composition comprises from or from about 5 to or to about 25 g/L glucose. In some embodiments, the cryopreservation composition comprises from or from about 5 to or to about 25, from or from about 5 to or to about 20, from or from about 5 to or to about 15, from or from about 5 to or to about 10, from or from about 10 to or to about 25, from or from about 10 to or to about 20, from or from about 10 to or to about 15, from or from about 15 to or to about 25, from or from about 15 to or to about 20, or from or from about 20 to or to about 25 g/L glucose. In some embodiments, the cryopreservation composition comprises 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, or 25 g/L glucose. In some embodiments, the cryopreservation composition comprises 12.5 g/L glucose. In some embodiments, the cryopreservation composition comprises about 5, about 7.5, about 10, about 12.5, about 15, about 17.5, about 20, about 22.5, or about 25 g/L glucose. In some embodiments, the cryopreservation composition comprises about 12.5 g/L glucose.

[0563] In some embodiments, the cryopreservation composition comprises less than 2.75% w/v glucose. In some embodiments, the cryopreservation composition comprises less than 27.5 g/L glucose. In some embodiments, the cryopreservation composition comprises less than 2% w/v glucose. In some embodiments, the cryopreservation composition comprises less than 1.5% w/v glucose. In some embodiments, the cryopreservation composition comprises about 1.25% w/v or less glucose.

Dimethyl Sulfoxide

[0564] In some embodiments, the cryopreservation composition comprises dimethyl sulfoxide (DMSO, also referred to as methyl sulfoxide and methylsulfinylmethane).

[0565] In some embodiments, the DMSO is provided as a solution, also referred to herein as a DMSO solution. Thus, in some embodiments, the cryopreservation composition comprises a DMSO solution.

[0566] In some embodiments, the DMSO solution is suitable for intravenous use.

[0567] In some embodiments, the DMSO solution comprises 1.1 g/mL DMSO. In some embodiments, the DMSO solution comprises about 1.1 g/mL DMSO.

[0568] In some embodiments, the cryopreservation composition comprises from or from about 1% to or to about 10% v/v of the DMSO solution. In some embodiments, the cryopreservation composition comprises from or from about 1% to or to about 10%, from or from about 1% to or to about 9%, from or from about 1% to or to about 8%, from or from about 1% to or to about 7%, from or from about 1% to or to about 6%, from or from about 1% to or to about 5%, from or from about 1% to or to about 4%, from or from about 1% to or to about 3%, from or from about 1% to or to about 2%, from or from about 2% to or to about 10%, from or from about 2% to or to about 9%, from or from about 8%, from or from about 2% to or to about 7%, from or from about 2% to or to about 6%, from or from about 2% to or to about 5%, from or from about 2% to or to about 4%, from or from about 2% to or to about 3%, from or from about 3% to or to about 10%, from or from about 3% to or to about 9%, from or from about 3% to or to about 8%, from or from about 3% to or to about 7%, from or from about 3% to or to about 6%, from or from about 3% to or to about 5%, from or from about 3% to or to about 4%, from or from about 4% to or to about 10%, from or from about 4% to or to about 9%, from or from about 4% to or to about 8%, from or from about 4% to or to about 7%, from or from about 4% to or to about 6%, from or from about 4% to or to about 5%, from or from about 5% to or to about 10%, from or from about 5% to or to about 9%, from or from about 5% to or to about 8%, from or from about 5% to or to about 7%, from or from about 5% to or to about 6%, from or from about 6% to or to about 10%, from or from about 6% to or to about 9%, from or from about 6% to or to about 8%, from or from about 6% to or to about 7%, from or from about 7% to or to about 10%, from or from about 7% to or to about 9%, from or from about 7% to or to about 8%, from or from about 8% to or to about 10%, from or from about 8% to or to about 9%, or from or from about 9% to or to about 10% v/v of the DMSO solution. In some embodiments, the cryopreservation composition comprises 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10% v/v of the DMSO solution. In some embodiments, the cryopreservation composition comprises 5% of the DMSO solution. In some embodiments, the cryopreservation composition comprises about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9%, or about 10% v/v of the DMSO solution. In some embodiments, the cryopreservation composition comprises about 5% of the DMSO solution.

[0569] In some embodiments, the cryopreservation composition comprises from or from about 11 to or to about 110 g/L DMSO. In some embodiments, from or from about the cryopreservation composition comprises from or from about 11 to or to about 110, from or from about 11 to or to about 99, from or from about 11 to or to about 88, from or from about 11 to or to about 77, from or from about 11 to or to about 66, from or from about 11 to or to about 55. from or from about 11 to or to about 44, from or from about 11 to or to about 33, from or from about 11 to or to about 22, from or from about 22 to or to about 110, from or from about 22 to or to about 99, from or from about 22 to or to about 88, from or from about 22 to or to about 77, from or from about 22 to or to about 77, from or from about 22 to or to about 66, from or from about 22 to or to about 55, from or from about 22 to or to about 44, from or from about 22 to or to about 33, from or from about 33 to or to about 110, from or from about 33 to or to about 99, from or from about 33 to or to about 88, from or from about 33 to or to about 77, from or from about 33 to or to about 66, from or from about 33 to or to about 55, from or from about 33 to or to about 44, from or from about 44 to or to about 110, from or from about 44 to or to about 99, from or from about 44 to or to about 88, from or from about 44 to or to about 77, from or from about 44 to or to about 66, from or from about 44 to or to about 55, from or from about 55 to or to about 110, from or from about 55 to or to about 99, from or from about 55 to or to about 88, from or from about 55 to or to about 77, from or from about 55 to or to about 66, from or from about 66 to or to about 110, from or from about 66 to or to about 99, from or from about 66 to or to about 88, from or from about 66 to or to about 77, from or from about 77 to or to about 119, from or from about 77 to or to about 88, from or from about 88 to or to about 110, from or from about 88 to or to about 99, or from or from about 99 to or to about 110 g/L DMSO. In some embodiments, the cryopreservation composition comprises 11, 22, 33, 44, 55, 66, 77, 88, 99, or 110 g/L DMSO. In some embodiments, the cryopreservation composition comprises 55 g/L DMSO. In some embodiments, the cryopreservation composition comprises about 11, about 22, about 33, about 44, about 55, about 66, about 77, about 88, about 99, or about 110 g/L DMSO. In some embodiments, the cryopreservation composition comprises about 55 g/L DMSO.

Buffers

[0570] In some embodiments, the cryopreservation composition comprises a buffer solution, e.g., a buffer solution suitable for intravenous administration.

[0571] Buffer solutions include, but are not limited to, phosphate buffered saline (PBS), Ringer's Solution, Tyrode's buffer, Hank's balanced salt solution, Earle's Balanced Salt Solution, saline, and Tris.

[0572] In some embodiments, the buffer solution is phosphate buffered saline (PBS).

Exemplary Cryopreservation Compositions

[0573] In some embodiments, the cryopreservation composition comprises or consists of: 1) albumin, e.g., human albumin, 2) dextran, e.g., Dextran 40, 3) DMSO, and 4) a buffer solution. In some embodiments, the cryopreservation composition further comprises glucose. In some embodiments, the cryopreservation composition consists of 1) albumin, e.g., human albumin, 2) dextran, e.g., Dextran 40. 3) glucose, 4) DMSO, and 5) a buffer solution.

[0574] In some embodiments, the cryopreservation composition comprises: 1) an albumin solution described herein, 2) a dextran solution described herein, 3) a DMSO solution described herein, and 4) a buffer solution.

[0575] In some embodiments, the cryopreservation composition consists of: 1) an albumin solution described herein, 2) a dextran solution described herein, 3) a DMSO solution described herein, and 4) a buffer solution.

[0576] In some embodiments, the cryopreservation composition does not comprise a cell culture medium.

[0577] In one embodiment, the cryopreservation composition comprises or comprises about 40 mg/mL human albumin, 25 mg/mL Dextran 40, 12.5 mg/mL glucose, and 55 mg/mL DMSO.

[0578] In one embodiment, the cryopreservation composition comprises or comprises about or consists of or consists of about 40 mg/mL human albumin, 25 mg/ml Dextran 40, 12.5 mg/mL glucose, 55 mg/mL DMSO, and 0.5 mL/mL 100% phosphate buffered saline (PBS) in water.

[0579] In one embodiment, the cryopreservation composition comprises or comprises about 32 mg/mL human albumin, 25 mg/mL Dextran 40, 12.5 mg/mL glucose, and 55 mg/mL DMSO.

[0580] In one embodiment, the cryopreservation composition comprises or comprises about or consists of or consists of about 32 mg/mL human albumin, 25 mg/ml Dextran 40, 12.5 mg/mL glucose, 55 mg/mL DMSO, and 0.54 mL/mL 100% phosphate buffered saline (PBS) in water.

[0581] Exemplary Cryopreservation Compositions are shown in Table 3, Table 4, Table 5, and Table 6.

TABLE-US-00003 TABLE 3 Exemplary Cryopreservation Compositions Exemplary Range v/v % in Concentration Range Exemplary Solution Cryopreservation Excipient Solution of Solution Concentration Composition Albumin Solution 40-200 g/L albumin 200 g/L albumin 10%-50% in water Dextran 40 25-200 g/L Dextran 100 g/L Dextran 40; 10%-50% Solution 40; and 0-100 g/L 50 g/L glucose glucose; in water DMSO 11-110 g/L DMSO 1,100 g/L DMSO 1%-10% in water Buffer to volume to volume to volume

TABLE-US-00004 TABLE 4 Exemplary Cryopreservation Composition #1 Exemplary v/v % in Final Concentration in Cryopreservation Cryopreservation Excipient Solution Solution Composition Composition #1 Composition #1 Albumin Solution 200 g/L albumin in water 20% 40 mg/mL albumin Dextran 40 100 g/L Dextran 40; and 25% 25 mg/mL Dextran 40; Solution 50 g/L glucose; in water 12.5 mg/mL glucose DMSO 100% DMSO (1,100 g/L) 5% 55 mg/mL Buffer 100% Phosphate 50% 0.5 mL/mL Buffered Saline (PBS)

TABLE-US-00005 TABLE 5 Exemplary Cryopreservation Composition #2 Exemplary v/v % in Final Concentration in Cryopreservation Cryopreservation Excipient Solution Solution Composition Composition #2 Composition #2 Albumin Solution 200 g/L albumin in water 16% 32 mg/mL albumin Dextran 40 100 g/L Dextran 40; and 25% 25 mg/mL Dextran 40; Solution 50 g/L glucose; in water 12.5 mg/mL glucose DMSO 100% DMSO (1,100 g/L) 5% 55 mg/mL Buffer 100% Phosphate 54% 0.54 mL/mL Buffered Saline (PBS)

TABLE-US-00006 TABLE 6 Exemplary Cryopreservation #3 Exemplary v/v % in Final Concentration in Cryopreservation Cryopreservation Excipient Solution Solution Composition Composition #3 Composition #3 Albumin Solution 250 g/L albumin in water 16% 40 mg/mL albumin Dextran 40 100 g/L Dextran 40; and 25% 25 mg/mL Dextran 40; Solution 50 g/L glucose; in water 12.5 mg/mL glucose DMSO 100% DMSO (1,100 g/L) 5% 55 mg/mL Buffer 100% Phosphate 54% 0.54 mL/mL Buffered Saline (PBS)

Methods of Cryopreserving

[0582] The cryopreservation compositions described herein can be used for cryopreserving cell(s), e.g., therapeutic cells, e.g., natural killer (NK) cell(s), e.g., the NK cell(s) described herein.

[0583] In some embodiments, the cell(s) are an animal cell(s). In some embodiments, the cell(s) are human cell(s).

[0584] In some embodiments, the cell(s) are immune cell(s). In some embodiments, the immune cell(s) are selected from basophils, eosinophils, neutrophils, mast cells, monocytes, macrophages, neutrophils, dendritic cells, natural killer cells, B cells, T cells, and combinations thereof.

[0585] In some embodiments, the immune cell(s) are natural killer (NK) cells. In some embodiments, the natural killer cell(s) are expanded and stimulated by a method described herein. In some embodiments, the NK cell(s) are CAR-NK cell(s), for example CAR-NK cell(s) described herein.

[0586] In some embodiments, cryopreserving the cell(s) comprises: mixing the cell(s) with a cryopreservation composition or components thereof described herein to produce a composition, e.g., a pharmaceutical composition; and freezing the mixture.

[0587] In some embodiments, cryopreserving the cell(s) comprises: mixing a composition comprising the cell(s) with a cryopreservation composition or components thereof described herein to produce a composition, e.g., a pharmaceutical composition; and freezing the mixture. In some embodiments, the composition comprising the cell(s) comprises: the cell(s) and a buffer. Suitable buffers are described herein.

[0588] In some embodiments, cryopreserving the cell(s) comprises: mixing a composition comprising the cell(s) and a buffer, e.g., PBS, with a composition comprising albumin. Dextran. and DMSO, e.g., as described herein; and freezing the mixture.

[0589] In some embodiments, cryopreserving the cell(s) comprises: mixing a composition comprising the cell(s) and a buffer, e.g., PBS 1:1 with a composition comprising 40 mg/mL albumin, e.g., human albumin, 25 mg/mL Dextran, e.g., Dextran 40, 12.5 mg/mL glucose and 55 mg/mL DMSO.

[0590] In some embodiments, the composition comprising the cell(s) and the buffer, e.g., . PBS, comprises from or from about 210.sup.7 to or to about 210.sup.9 cells/mL. In some embodiments, the composition comprising the cell(s) and the buffer, e.g., PBS, comprises 210.sup.8 cells/mL. In some embodiments, the composition comprising the cell(s) and the buffer, e.g., PBS, comprising about 210.sup.8 cells/mL.

[0591] In some embodiments, cryopreserving the cell(s) comprising mixing: the cell(s), a buffer, e.g., PBS, albumin, e.g., human albumin, Dextran, e.g., Dextran 40, and DMSO; and freezing the mixture.

[0592] In some embodiments, the mixture comprises from or from about 110.sup.7 to or to about 110.sup.9 cells/mL. In some embodiments, the mixture comprises 110.sup.8 cells/mL. In some embodiments, the mixture comprises about 110.sup.8 cells/mL.

[0593] Suitable ranges for albumin, Dextran, and DMSO are set forth above.

[0594] In some embodiments, the composition is frozen at or below135 C.

[0595] In some embodiments, the composition is frozen at a controlled rate.

Pharmaceutical Compositions

[0596] Provided herein are pharmaceutical compositions comprising the natural killer cells described herein and dosage units of the pharmaceutical compositions described herein.

[0597] In some cases, the dosage unit comprises between 100 million and 1.5 billion cells, e.g., 100 million, 200 million, 300 million, 400 million, 500 million, 600 million, 700 million, 800 million, 900 million, 1 billion, 1.1 billion, 1.2 billion, 1.3 billion, 1.4 billion, or 1.5 billion.

[0598] Pharmaceutical compositions typically include a pharmaceutically acceptable carrier. As used herein the language pharmaceutically acceptable carrier includes saline, solvents. dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration.

[0599] In some embodiments, the pharmaceutical composition comprises: a) natural killer cell(s) described herein; and b) a cryopreservation composition.

[0600] Suitable cryopreservation compositions are described herein.

[0601] In some embodiments, the composition is frozen. In some embodiments, the composition has been frozen for at least three months, e.g., at least six months, at least nine months, at least 12 months, at least 15 months, at least 18 months, at least 24 months, or at least 36 months.

[0602] In some embodiments, at least 60%, e.g., at least 70%, at least 80%, at least 90% at least 95%, at least 99%, or 100% of the natural killer cells are viable after being thawed.

[0603] In some embodiments, the pharmaceutical composition comprises: a) a cryopreservation composition described herein; and b) therapeutic cell(s).

[0604] In some embodiments, the therapeutic cell(s) are animal cell(s). In some embodiments, the therapeutic cell(s) are human cell(s).

[0605] In some embodiments, the therapeutic cell(s) are immune cell(s). In some embodiments, the immune cell(s) are selected from basophils, eosinophils, neutrophils, mast cells, monocytes, macrophages, neutrophils, dendritic cells, natural killer cells, B cells, T cells, and combinations thereof.

[0606] In some embodiments, the immune cell(s) are natural killer (NK) cells. In some embodiments, the natural killer cell(s) are expanded and stimulated by a method described herein, e.g., the CAR-NKs described herein.

[0607] In some embodiments, the pharmaceutical composition further comprises: c) a buffer solution. Suitable buffer solutions are described herein, e.g., as for cryopreservation compositions.

[0608] In some embodiments, the pharmaceutical composition comprises from or from about

[0609] 110.sup.7 to or to about 110.sup.9 cells/mL. In some embodiments, the pharmaceutical composition comprises 110.sup.8 cells/mL. In some embodiments, the pharmaceutical composition comprises about 110.sup.8 cells/mL.

[0610] In some embodiments, the pharmaceutical composition further comprises an antibody or antigen binding fragment thereof, e.g., an antibody described herein.

[0611] Pharmaceutical compositions are typically formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, oral (e.g., inhalation), transdermal (topical), transmucosal, and rectal administration.

[0612] Methods of formulating suitable pharmaceutical compositions are known in the art, see, e.g., Remington: The Science and Practice of Pharmacy, 21st ed., 2005; and the books in the series Drugs and the Pharmaceutical Sciences: a Series of Textbooks and Monographs (Dekker, NY). For example, solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents: antibacterial agents such as benzyl alcohol or methyl parabens: antioxidants such as ascorbic acid or sodium bisulfite: chelating agents such as ethylenediaminetetraacetic acid: buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose, pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.

[0613] Pharmaceutical compositions suitable for injectable use can include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL (BASF, Parsippany, NJ) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringability exists. It should be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent that delays absorption, for example, aluminum monostearate and gelatin.

[0614] Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required. followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle, which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying, which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

Methods of Treatment

[0615] The NK cells described herein, e.g., the CAR-NK cells described herein, find use for treating cancer or other proliferative disorders.

[0616] Thus, also provided herein are methods of treating a patient suffering from a disorder, e.g., a disorder associated with a cancer, e.g., a HER2+ cancer, comprising administering the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein.

[0617] Also provided herein are methods of preventing, reducing and/or inhibiting the recurrence, growth, proliferation, migration and/or metastasis of a cancer cell or population of cancer cells in a subject in need thereof, comprising administering the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein.

[0618] Also provided herein are methods of enhancing, improving, and/or increasing the response to an anticancer therapy in a subject in need thereof, comprising administering the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein.

[0619] Also provided herein are methods for inducing the immune system in a subject in need thereof comprising administering the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein.

[0620] The methods described herein include methods for the treatment of disorders associated with abnormal apoptotic or differentiative processes, e.g., cellular proliferative disorders or cellular differentiative disorders, e.g., cancer, including both solid tumors and hematopoietic cancers. Generally, the methods include administering a therapeutically effective amount of a treatment as described herein, to a subject who is in need of, or who has been determined to be in need of, such treatment. In some embodiments, the methods include administering a therapeutically effective amount of a treatment comprising NK cells, e.g., CAR-NK cells described herein.

[0621] As used herein, the terms treatment, treat. and treating refer to reversing, alleviating, delaying the onset of, or inhibiting the progress of a disorder associated with abnormal apoptotic or differentiative processes. For example, a treatment can result in a reduction in tumor size or growth rate. Administration of a therapeutically effective amount of a compound described herein for the treatment of a condition associated with abnormal apoptotic or differentiative processes will result in a reduction in tumor size or decreased growth rate, a reduction in risk or frequency of reoccurrence, a delay in reoccurrence, a reduction in metastasis. increased survival, and/or decreased morbidity and mortality, among other things. In some embodiments, treatment may be administered after one or more symptoms have developed. In other embodiments, treatment may be administered in the absence of symptoms. For example, treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example to prevent or delay their recurrence.

[0622] As used herein, the terms inhibition, as it relates to cancer and/or cancer cell proliferation, refer to the inhibition of the growth, division, maturation or viability of cancer cells, and/or causing the death of cancer cells, individually or in aggregate with other cancer cells, by cytotoxicity, nutrient depletion, or the induction of apoptosis.

[0623] As used herein, delaying development of a disease or disorder, or one or more symptoms thereof, means to defer, hinder, slow, retard, stabilize and/or postpone development of the disease, disorder, or symptom thereof. This delay can be of varying lengths of time, depending on the history of the disease and/or subject being treated. As is evident to one skilled in the art, a sufficient or significant delay can, in effect, encompass prevention, in that the subject does not develop the disease, disorder, or symptom thereof. For example, a method that delays development of cancer is a method that reduces the probability of disease development in a given time frame and/or reduces extent of the disease in a given time frame, when compared to not using the method. Such comparisons may be based on clinical studies, using a statistically significant number of subjects.

[0624] As used herein, prevention or preventing refers to a regimen that protects against the onset of the disease or disorder such that the clinical symptoms of the disease do not develop. Thus, prevention relates to administration of a therapy (e.g., administration of a therapeutic substance) to a subject before signs of the disease are detectable in the subject and/or before a certain stage of the disease (e.g., administration of a therapeutic substance to a subject with a cancer that has not yet metastasized). The subject may be an individual at risk of developing the disease or disorder, or at risk of disease progression, e.g., cancer metastasis. Such as an individual who has one or more risk factors known to be associated with development or onset of the disease or disorder. For example, an individual may be have mutations associated with the development or progression of a cancer. Further, it is understood that prevention may not result in complete protection against onset of the disease or disorder. In some instances, prevention includes reducing the risk of developing the disease or disorder. The reduction of the risk may not result in complete elimination of the risk of developing the disease or disorder.

[0625] An increased or enhanced amount (e.g., with respect to antitumor response, cancer cell metastasis) refers to an increase that is 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6, 7, 8, 9, 10, 15. 20, 30, 40, or 50 or more times (e.g., 100, 500, 1000 times) (including all integers and decimal points in between and above 1, e.g., 2.1, 2.2, 2.3, 2.4, etc.) an amount or level described herein. It may also include an increase of at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 100%, at least 150%, at least 200%, at least 500%, or at least 1000% of an amount or level described herein.

[0626] A decreased or reduced or lesser amount (e.g., with respect to tumor size, cancer cell proliferation or growth) refers to a decrease that is about 1.1, 1.2, 1.3, 1.4, 1.5, 1.6 1.7, 1.8, 1.9, 2, 2.5, 3, 3.5, 4, 4.5. 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, or 50 or more times (e.g., 100, 500, 1000 times) (including all integers and decimal points in between and above 1, e.g., 1.5, 1.6, 1.7. 1.8, etc.) an amount or level described herein. It may also include a decrease of at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, or at least 90%, at least 100%, at least 150%, at least 200%, at least 500%, or at least 1000% of an amount or level described herein.

Disorders

[0627] Methods and manufactured compositions disclosed herein find use in targeting a number of disorders, such as cellular proliferative disorders. A benefit of the approaches herein is that allogenic cells are used to target specific cells. Unlike previous therapies, such as chemotherapy or radiotherapy, using the approaches and pharmaceutical compositions herein, one is able to specifically target cells exhibiting detrimental proliferative activity, potentially without administering a systemic drug or toxin that impacts proliferating cells indiscriminately.

[0628] Examples of cellular proliferative and/or differentiative disorders include cancer, e.g., carcinoma, sarcoma, metastatic disorders or hematopoietic neoplastic disorders, e.g., leukemias. A metastatic tumor can arise from a multitude of primary tumor types, including but not limited to those of prostate, colon, lung, breast and liver origin.

[0629] As used herein, the terms cancer, hyperproliferative and neoplastic refer to cells having the capacity for autonomous growth, i.e., an abnormal state or condition characterized by rapidly proliferating cell growth. Hyperproliferative and neoplastic disease states may be categorized as pathologic, i.e., characterizing or constituting a disease state, or may be categorized as non-pathologic, i.e., a deviation from normal but not associated with a disease state. The term is meant to include all types of cancerous growths or oncogenic processes, metastatic tissues or malignantly transformed cells, tissues, or organs, irrespective of histopathologic type or stage of invasiveness. Pathologic hyperproliferative cells occur in disease states characterized by malignant tumor growth. Examples of non-pathologic hyperproliferative cells include proliferation of cells associated with wound repair.

[0630] The terms cancer or neoplasms include malignancies of the various organ systems, such as affecting lung, breast, thyroid, lymphoid, gastrointestinal, and genito-urinary tract, as well as adenocarcinomas which include malignancies such as most colon cancers, renal-cell carcinoma, prostate cancer and/or testicular tumors, non-small cell carcinoma of the lung, cancer of the small intestine and cancer of the esophagus.

[0631] The term carcinoma is art recognized and refers to malignancies of epithelial or endocrine tissues including respiratory system carcinomas, gastrointestinal system carcinomas, genitourinary system carcinomas, testicular carcinomas, breast carcinomas, prostatic carcinomas, endocrine system carcinomas, and melanomas. In some embodiments, the disease is renal carcinoma or melanoma. Exemplary carcinomas include those forming from tissue of the cervix.

[0632] lung, prostate, breast. head and neck, colon and ovary. The term also includes carcinosarcomas, e.g., which include malignant tumors composed of carcinomatous and sarcomatous tissues. An adenocarcinoma refers to a carcinoma derived from glandular tissue or in which the tumor cells form recognizable glandular structures.

[0633] The term sarcoma is art recognized and refers to malignant tumors of mesenchymal derivation.

[0634] Additional examples of proliferative disorders include hematopoietic neoplastic disorders. As used herein, the term hematopoietic neoplastic disorders includes diseases involving hyperplastic/neoplastic cells of hematopoietic origin, e.g., arising from myeloid, lymphoid or erythroid lineages, or precursor cells thereof. Preferably, the diseases arise from poorly differentiated acute leukemias, e.g., erythroblastic leukemia and acute megakaryoblastic leukemia. Additional exemplary myeloid disorders include, but are not limited to, acute promyeloid leukemia (APML), acute myelogenous leukemia (AML) and chronic myelogenous leukemia (CML) (reviewed in Vaickus, L. (1991) Crit Rev, in Oncol./Hemotol. 11:267-97): lymphoid malignancies include, but are not limited to acute lymphoblastic leukemia (ALL) which includes B-lineage ALL and T-lineage ALL, chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PLL), hairy cell leukemia (HLL) and Waldenstrom's macroglobulinemia (WM). Additional forms of malignant lymphomas include, but are not limited to non-Hodgkin lymphoma and variants thereof, peripheral T cell lymphomas, adult T cell leukemia/lymphoma (ATL), cutaneous T-cell lymphoma (CTCL), large granular lymphocytic leukemia (LGF), Hodgkin's disease and Reed-Sternberg disease.

[0635] In some embodiments, the cancer is selected from the group consisting of: acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), adrenocortical carcinoma. Kaposi sarcoma, AIDS-related lymphoma, primary CNS lymphoma, anal cancer, appendix cancer, astrocytoma, typical teratoid/rhabdoid tumor, basal cell carcinoma, bile duct cancer, bladder cancer, bone cancer, brain tumor, breast cancer, bronchial tumor, Burkitt lymphoma, carcinoid, cardiac tumors, medulloblastoma, germ cell tumor, primary CNS lymphoma, cervical cancer, cholangiocarcinoma, chordoma, chronic lymphocytic leukemia (CLL), chronic myelogenous leukemia (CML), chronic myeloproliferative neoplasms, colorectal cancer, craniopharyngioma, cutaneous T-cell lymphoma, ductal carcinoma in situ, embryonal tumors, endometrial cancer, ependymoma, esophageal cancer, esthesioneuroblastoma, Ewing sarcoma, extracranial germ cell tumor, extragonadal germ cell tumor, eye cancer (e.g., intraocular melanoma or retinoblastoma), fallopian tube cancer, fibrous histiocytoma of bone, osteosarcoma, gallbladder cancer, gastric cancer, gastrointestinal carcinoid tumor, gastrointestinal stromal tumors (GIST), germ cell tumors, gestational trophoblastic disease, hairy cell leukemia, head and neck cancer, heart tumor. hepatocellular cancer, histiocytosis, Hodgkin lymphomas, hypopharyngeal cancer, intraocular melanoma, islet cell tumors, pancreatic neuroendocrine tumors, kidney (renal cell) carcinoma, Langerhans cell histiocytosis, laryngeal cancer, leukemia, lip and oral cavity cancer, liver cancer, lung cancer (e.g., non-small cell lung cancer, small cell lung cancer, pleuropulmonary blastoma, and tracheobronchial tumor), lymphoma, male breast cancer, malignant fibrous histiocytoma of bone, melanoma, Merkel cell carcinoma, mesothelioma, metastatic cancer, metastatic squamous neck cancer, midline tract carcinoma, mouth cancer, multiple endocrine neoplasia syndromes, multiple myeloma/plasma cell neoplasms, mycosis fungoides, myelodysplastic syndromes, myelodysplastic/myeloproliferative neoplasms, myeloproliferative neoplasms, nasal cavity and paranasal sinus cancer, nasopharyngeal cancer, neuroblastoma, non-Hodgkin lymphoma, oral cancer, lip and oral cavity cancer, oropharyngeal cancer, osteosarcoma, malignant fibrous histiocytoma, ovarian cancer, pancreatic cancer, pancreatic neuroendocrine tumors, papillomatosis, paraganglioma, paranasal sinus and nasal cavity cancer, parathyroid cancer, penile cancer, pharyngeal cancer, pheochromocytomas, pituitary tumor, plasma cell neoplasm, multiple myeloma, pleuropulmonary blastoma, pregnancy and breast cancer, primary central nervous system lymphoma, primary peritoneal cancer, prostate cancer, rectal cancer, recurrent cancer, renal cell cancer, retinoblastoma, rhabdomyosarcoma, salivary gland cancer, sarcoma (e.g., childhood rhabdomyosarcoma, childhood vascular tumors, Ewing sarcoma, Kaposi sarcoma, osteosarcoma, soft tissue sarcoma, uterine sarcoma), Sezary syndrome, skin cancer, small intestine cancer, soft tissue sarcoma, squamous cell carcinoma, squamous neck cancer, stomach cancer, T-cell lymphomas, testicular cancer, throat cancer, nasopharyngeal cancer, oropharyngeal cancer, hypopharyngeal cancer, thryomoma and thymic carcinomas, thyroid cancer, tracheobronchial tumors, transitional cell cancer of the renal pelvis and ureter, urethral cancer, uterine cancer, uterine sarcoma, vaginal cancer, vascular tumors, vulvar cancer, and Wilms tumor.

[0636] In some embodiments, the cancer is a solid tumor.

[0637] In some embodiments, the cancer is metastatic.

[0638] In some embodiments, the cancer is a HER2+ cancer.

[0639] In some embodiments, the HER2+ cancer is selected from the group consisting of bladder cancer, breast adenocarcinoma, colorectal adenocarcinoma, non-small cell lung cancer, esophageal cancer, cervix squamous cancer, stomach adenocarcinoma, cholangiocarcinoma, ovary cancer, renal papillary cell carcinoma, and combinations thereof.

[0640] In some embodiments, the HER2+ cancer is selected from the group consisting of breast cancer, gastric cancer, and ovarian cancer.

[0641] In some embodiments, the HER2+ cancer is breast cancer. In some embodiments, the HER2+ cancer is gastric cancer. In some embodiments, the HER2+ cancer is ovarian cancer.

Patients

[0642] Suitable patients for the compositions and methods herein include those who are suffering from, who have been diagnosed with, or who are suspected of having a cellular proliferative and/or differentiative disorder, e.g., a cancer. Patients subjected to technology of the disclosure herein generally respond better to the methods and compositions herein, in part because the pharmaceutical compositions are allogeneic and target cells identified by the antigen binding domain, rather than targeting proliferating cells generally. As a result, there is less off-target impact and the patients are more likely to complete treatment regimens without substantial detrimental off-target effects.

[0643] In some embodiments, the methods of treatment provided herein may be used to treat a subject (e.g., human, monkey, dog, cat, mouse) who has been diagnosed with or is suspected of having a cellular proliferative and/or differentiative disorder, e.g., a cancer. In some embodiments, the subject is a mammal. In some embodiments, the subject is a human.

[0644] As used herein, a subject refers to a mammal, including, for example, a human.

[0645] In some embodiments, the mammal is selected from the group consisting of an armadillo, an ass, a bat, a bear, a beaver, a cat, a chimpanzee, a cow, a coyote, a deer, a dog, a dolphin, an elephant, a fox, a panda, a gibbon, a giraffe, a goat, a gopher, a hedgehog, a hippopotamus, a horse, a humpback whale, a jaguar, a kangaroo, a koala, a leopard, a lion, a llama, a lynx, a mole, a monkey, a mouse, a narwhal, an orangutan, an orca, an otter, an ox, a pig, a polar bear, a porcupine, a puma, a rabbit, a raccoon, a rat, a rhinoceros, a sheep, a squirrel, a tiger, a walrus, a weasel, a wolf, a zebra, a goat, a horse, and combinations thereof.

[0646] In some embodiments, the mammal is a human.

[0647] The subject, e.g., the human subject, can be a child, e.g., from or from about ( ) to or to about 14 years in age. The subject can be a youth, e.g., from or from about 15 to or to about 24 years in age. The subject can be an adult, e.g., from or from about 25 to or to about 64 years in age. The subject can be a senior, e.g. 65+ years in age.

[0648] In some embodiments, the subject may be a human who exhibits one or more symptoms associated with a cellular proliferative and/or differentiative disorder, e.g., a cancer, e.g., a tumor. Any of the methods of treatment provided herein may be used to treat cancer at various stages. By way of example, the cancer stage includes but is not limited to early, advanced, locally advanced, remission, refractory, reoccurred after remission and progressive. In some embodiments, the subject is at an early stage of a cancer. In other embodiments, the subject is at an advanced stage of cancer. In various embodiments, the subject has a stage I, stage II, stage III or stage IV cancer. The methods of treatment described herein can promote reduction or retraction of a tumor, decrease or inhibit tumor growth or cancer cell proliferation, and/or induce, increase or promote tumor cell killing. In some embodiments, the subject is in cancer remission. The methods of treatment described herein can prevent or delay metastasis or recurrence of cancer.

[0649] In some embodiments, the subject has an Eastern Cooperative Oncology Group (ECOG) performance status of 0, 1, 2, 3, or 4 at the time of treatment. In some embodiments, the subject has an ECOG performance status of 0 to 4, 0 to 3, 0 to 2, 0 to 1, 1 to 4, 1 to 3, 1 to 2, 2 to 4, 2 to 3, or 3 to 4 at the time of treatment.

[0650] In some embodiments, the subject is at risk, or genetically or otherwise predisposed (e.g., risk factor), to developing a cellular proliferative and/or differentiative disorder, e.g., a cancer, that has or has not been diagnosed.

[0651] As used herein, an at risk individual is an individual who is at risk of developing a condition to be treated, e.g., a cellular proliferative and/or differentiative disorder, e.g., a cancer. Generally, an at risk subject may or may not have detectable disease, and may or may not have displayed detectable disease prior to the treatment methods described herein. At risk denotes that an individual has one or more so-called risk factors, which are measurable parameters that correlate with development of a disease or condition and are known in the art. For example, an at risk subject may have one or more risk factors, which are measurable parameters that correlate with development of cancer. A subject having one or more of these risk factors has a higher probability of developing cancer than an individual without these risk factor(s). In general, risk factors may include, for example, age, sex, race, diet, history of previous disease, presence of precursor disease, genetic (e.g., hereditary) considerations, and environmental exposure. In some embodiments, the subjects at risk for cancer include, for example, those having relatives who have experienced the disease, and those whose risk is determined by analysis of genetic or biochemical markers.

[0652] In addition, the subject may be undergoing one or more standard therapies, such as chemotherapy, radiotherapy, immunotherapy, surgery, or combination thereof. Accordingly, one or more kinase inhibitors may be administered before, during, or after administration of chemotherapy, radiotherapy, immunotherapy, surgery or combination thereof.

[0653] In certain embodiments, the subject may be a human who is (i) substantially refractory to at least one chemotherapy treatment, or (ii) is in relapse after treatment with chemotherapy, or both (i) and (ii). In some of embodiments, the subject is refractory to at least two, at least three, or at least four chemotherapy treatments (including standard or experimental chemotherapies).

[0654] In some embodiments, the subject has a cancer that expresses HER2.

[0655] In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer.

[0656] In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer by immunohistochemical staining of a biopsy or surgical sample of the cancer. In some embodiments, the patient is or has been diagnosed with a HER2+ cancer by fluorescent in situ hybridization of a biopsy or surgical sample of the cancer.

[0657] In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer according to ASCOR Guidelines, e.g., the 2018 ASCOR Guidelines, e.g., as described in Wolff et al., Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med 142:1364-82 (2018), which is hereby incorporated by reference in its entirety

[0658] In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer with an IHC score of 2+, according to ASCOR Guidelines, e.g., the 2018

[0659] ASCOR Guidelines, e.g., as described in Wolff et al., Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med 142:1364-82 (2018), which is hereby incorporated by reference in its entirety. In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer with an IHC score of 2+ within 12 months (e.g., within 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2, or 1 months) of treatment.

[0660] In some embodiments, the patient has a cancer that is not HER2-addicted, but shows some degree of HER2-expression. In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2-low cancer. In some embodiments, the HER2-low cancer has a HER2 expression classification of score 1+ or 2+ according to ASCOR Guidelines, e.g., the 2018 ASCOR Guidelines, e.g., as described in Wolff et al., Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med 142:1364-82 (2018), which is hereby incorporated by reference in its entirety, but lacks HER2 amplification. In some embodiments, the HER2-low cancer harbors HER2 gain. In some embodiments, the HER2-low cancer does not harbor HER2 gain.

[0661] In some embodiments, the patient is diagnosed with or has been diagnosed with a HER2+ cancer by genetic analysis, e.g., by identifying a HER2 mutated cancer, e.g., a somatic mutation in the HER2 (ERBB2) gene.

[0662] In some embodiments, the patient has a cancer comprising one or more mutations set forth in Table 7, an insertion or deletion polymorphism in the HER2 gene, a copy number variation of the HER2 gene, a methylation mutation of the HER2 gene, or combinations thereof. In some embodiments, the mutation is a HER2 activating mutation.

[0663] In some embodiments, the patient has a chromosomal translocation associated with cancer, e.g., a HER2+ cancer. In some embodiments, the patient has a fusion gene associated with cancer, e.g., a HER+ cancer.

TABLE-US-00007 TABLE 7 HER2 (ERBB2) Mutations (relative to Human Genome Assembly Reference Build GRCh38.p13 (ncbi.nlm.nih.gov/assembly/88331) Mutation (GRCh38) Protein Position Consequence 17: 39711955: C > T 310 missense_variant 17: 39723405: G > A 678 missense_variant 17: 39723967: T > C 755 missense_variant 17: 39725079: G > A 842 missense_variant 17: 39711955: C > A 310 missense_variant 17: 39724747: G > T 777 missense_variant 17: 39724008: G > T 769 missense_variant 17: 39724004: C > G 767 missense_variant 17: 39725139: A > G 862 missense_variant 17: 39724745: G > T 776 missense_variant 17: 39723650: C > T 733 missense_variant 17: 39724008: G > C 769 missense_variant 17: 39724747: G > C 777 missense_variant 17: 39725161: T > G 869 missense_variant 17: 39710410: A > G 277 missense_variant 17: 39710414: G > A 278 synonymous_variant 17: 39711963: C > G 313 missense_variant 17: 39723966: T > C 755 synonymous_variant 17: 39724008: G > A 769 missense_variant 17: 39726610: C > T 974 missense_variant 17: 39727303: T > G 1056 synonymous_variant 17: 39727784: C > G 1170 missense_variant 17: 39706987: C > T splice_region_variant 17: 39708375: G > A 94 missense_variant 17: 39708497: A > T 134 synonymous_variant 17: 39708530: C > T 145 synonymous_variant 17: 39709376: G > A 166 synonymous_variant 17: 39709810: C > T splice_region_variant 17: 39710106: G > T 222 missense_variant 17: 39710164: C > T 241 missense_variant 17: 39710409: G > T 277 missense_variant 17: 39710418: G > C 280 missense_variant 17: 39712319: C > T splice_region_variant 17: 39712429: C > T 377 synonymous_variant 17: 39715854: C > T 476 synonymous_variant 17: 39716421: G > A 545 missense_variant 17: 39716586: C > T 573 missense_variant 17: 39717397: A > C 605 missense_variant 17: 39717446: G > A 622 missense_variant 17: 39719819: C > T 644 missense_variant 17: 39719827: A > C 647 synonymous_variant 17: 39723330: C > G 653 missense_variant 17: 39723339: C > T 656 missense_variant 17: 39723530: C > T splice_region_variant 17: 39724734: C > T 772 synonymous_variant 17: 39724902: G > A 828 synonymous_variant 17: 39725057: C > T 834 synonymous_variant 17: 39725096: C > T 847 synonymous_variant 17: 39725139: A > T 862 missense_variant 17: 39725347: G > A 890 synonymous_variant 17: 39725357: A > C 894 missense_variant 17: 39725750: C > T 923 synonymous_variant 17: 39726573: G > A 962 missense_variant 17: 39726860: C > T 1006 missense_variant 17: 39726905: G > C 1021 missense_variant 17: 39727000: C > G 1052 synonymous_variant 17: 39727847: G > A 1191 missense_variant 17: 39727973: C > T 1233 missense_variant 17: 39700256: G > T 6 missense_variant 17: 39700298: C > T 20 synonymous_variant 17: 39706995: A > T 27 missense_variant 17: 39707022: C > T 36 missense_variant 17: 39707032: C > T 39 missense_variant 17: 39707033: C > T 39 synonymous_variant 17: 39707063: C > T 49 synonymous_variant 17: 39707070: G > A 52 missense_variant 17: 39707076: C > T 54 stop_gained 17: 39707093: C > A 59 missense_variant 17: 39707114: C > A 66 synonymous_variant 17: 39708350: C > G 85 synonymous_variant 17: 39708354: G > A 87 missense_variant 17: 39708386: G > A 97 synonymous_variant 17: 39708397: T > G 101 missense_variant 17: 39708403: G > A 103 missense_variant 17: 39708406: G > A 104 missense_variant 17: 39708459: C > T 122 missense_variant 17: 39708460: C > T 122 missense_variant 17: 39708507: C > T 138 missense_variant 17: 39708508: G > A 138 missense_variant 17: 39708510: G > A 139 missense_variant 17: 39709322: C > A 148 synonymous_variant 17: 39709326: A > G 150 missense_variant 17: 39709340: G > C 154 missense_variant 17: 39709343: C > T 155 synonymous_variant 17: 39709352: C > T 158 synonymous_variant 17: 39709375: C > T 166 missense_variant 17: 39709391: C > T 171 synonymous_variant 17: 39709394: C > G 172 missense_variant 17: 39709419: C > T 181 missense_variant 17: 39709421: C > G 181 synonymous_variant 17: 39709423: C > T 182 missense_variant 17: 39709427: G > C 183 synonymous_variant 17: 39709447: G > A 190 missense_variant 17: 39709449: G > A 191 missense_variant 17: 39709824: T > C 196 missense_variant 17: 39709825: C > T 196 missense_variant 17: 39709845: C > T 203 missense_variant 17: 39709850: C > A 204 stop_gained 17: 39710087: G > T 215 splice_region_variant 17: 39710097: G > A 219 missense_variant 17: 39710099: C > A 219 synonymous_variant 17: 39710103: G > A 221 missense_variant 17: 39710111: C > A 223 synonymous_variant 17: 39710120: C > T 226 synonymous_variant 17: 39710154: G > A 238 missense_variant 17: 39710182: C > T 247 missense_variant 17: 39710184: A > G 248 missense_variant 17: 39710191: C > T 250 missense_variant 17: 39710209: C > T splice_region_variant 17: 39710418: G > A 280 missense_variant 17: 39710433: C > T 285 missense_variant 17: 39710454: G > C 292 missense_variant 17: 39710458: C > T 293 missense_variant 17: 39710480: C > T 300 splice_region_variant 17: 39711940: C > G 305 missense_variant 17: 39711950: G > A 308 synonymous_variant 17: 39711952: G > C 309 missense_variant 17: 39711954: T > G 310 missense_variant 17: 39711962: C > G 312 synonymous_variant 17: 39711981: A > T 319 missense_variant 17: 39712006: G > A 327 missense_variant 17: 39712012: A > T 329 missense_variant 17: 39712014: C > T 330 missense_variant 17: 39712044: C > G 340 missense_variant 17: 39712323: G > T 341 splice_region_variant 17: 39712361: G > 354 frameshift_variant 17: 39712431: G > A 377 synonymous_variant 17: 39715322: G > C 395 missense_variant 17: 39715328: C > T 397 synonymous_variant 17: 39715340: G > A 401 synonymous_variant 17: 39715344: C > T 403 synonymous_variant 17: 39715352: G > C 405 missense_variant 17: 39715445: G > T splice_acceptor_variant 17: 39715461: C > T 413 missense_variant 17: 39715476: G > C 418 missense_variant 17: 39715478: C > T 419 synonymous_variant 17: 39715519: G > A 432 synonymous_variant 17: 39715519: G > T 432 synonymous_variant 17: 39715738: A > T splice_acceptor_variant 17: 39715744: G > A 440 missense_variant 17: 39715766: G > C 447 missense_variant 17: 39715783: C > A 453 missense_variant 17: 39715804: G > A 460 missense_variant 17: 39715830: C > T 468 synonymous_variant 17: 39715836: T > G 470 missense_variant 17: 39715844: A > T 473 missense_variant 17: 39715867: C > 481 frameshift_variant 17: 39716306: G > A 507 missense_variant 17: 39716354: C > T 523 missense_variant 17: 39716365: C > G 526 synonymous_variant 17: 39716377: C > T 530 synonymous_variant 17: 39716393: C > T 536 missense_variant 17: 39716401: G > A 538 synonymous_variant 17: 39716418: G > T 544 missense_variant 17: 39716552: C > T 562 missense_variant 17: 39716592: C > T 575 missense_variant 17: 39717326: G > A 582 missense_variant 17: 39717351: A > G 590 missense_variant 17: 39717358: C > T 592 synonymous_variant 17: 39717367: C > A 595 missense_variant 17: 39717376: C > T 598 synonymous_variant 17: 39717406: C > T 608 synonymous_variant 17: 39717439: G > A 619 synonymous_variant 17: 39717442: G > 620 frameshift_variant 17: 39717466: C > G 628 missense_variant 17: 39717480: C > T 633 missense_variant 17: 39719785: A > T splice_acceptor_variant 17: 39719786: G > C splice_acceptor_variant 17: 39719799: G > A 637 synonymous_variant 17: 39719811: C > T 641 synonymous_variant 17: 39719820: C > T 644 synonymous_variant 17: 39719834: G > C 649 missense_variant 17: 39723323: C > G 651 missense_variant 17: 39723328: G > T 652 synonymous_variant 17: 39723334: C > A 654 synonymous_variant 17: 39723335: A > G 655 missense_variant 17: 39723350: G > C 660 missense_variant 17: 39723351: G > A 660 missense_variant 17: 39723356: C > G 662 missense_variant 17: 39723356: C > T 662 synonymous_variant 17: 39723357: T > A 662 missense_variant 17: 39723360: T > C 663 missense_variant 17: 39723368: G > T 666 missense_variant 17: 39723373: G > C 667 missense_variant 17: 39723376: G > T 668 synonymous_variant 17: 39723397: C > G 675 missense_variant 17: 39723402: G > A 677 missense_variant 17: 39723412: G > A 680 synonymous_variant 17: 39723449: G > A 693 missense_variant 17: 39723541: G > T 697 missense_variant 17: 39723562: G > A 704 missense_variant 17: 39723567: G > A 705 synonymous_variant 17: 39723577: C > G 709 missense_variant 17: 39723582: G > A 710 synonymous_variant 17: 39723594: C > T 714 synonymous_variant 17: 39723597: G > T 715 synonymous_variant 17: 39723603: G > C 717 missense_variant 17: 39723908: C > T splice_region_variant 17: 39723909: C > T splice_region_variant 17: 39723916: T > A 738 missense_variant 17: 39723941: G > A 746 synonymous_variant 17: 39723966: T > A 755 missense_variant 17: 39723967: T > G 755 missense_variant 17: 39723970: G > A 756 missense_variant 17: 39723974: A > C 757 missense_variant 17: 39724002: A > T 767 missense_variant 17: 39724010: C > T 769 splice_region_variant 17: 39724743: G > ATCT 775 inframe_insertion 17: 39724744: G > A 776 missense_variant 17: 39724761: T > C 781 synonymous_variant 17: 39724768: C > T 784 missense_variant 17: 39724774: C > T 786 synonymous_variant 17: 39724780: A > T 788 missense_variant 17: 39724784: G > A 789 missense_variant 17: 39724822: C > T 802 missense_variant 17: 39724874: C > T 819 missense_variant 17: 39724879: G > C 821 missense_variant 17: 39724882: C > T 822 synonymous_variant 17: 39725070: G > A 839 missense_variant 17: 39725088: G > A 845 missense_variant 17: 39725122: C > G 856 missense_variant 17: 39725172: G > A 873 missense_variant 17: 39725174: C > T 873 synonymous_variant 17: 39725177: G > A 874 synonymous_variant 17: 39725187: C > T 878 missense_variant 17: 39725192: A > G 879 synonymous_variant 17: 39725344: G > A 889 missense_variant 17: 39725363: C > T 896 missense_variant 17: 39725374: C > A 899 missense_variant 17: 39725374: C > G 899 missense_variant 17: 39725400: A > G 908 missense_variant 17: 39725726: G > C 915 synonymous_variant 17: 39725729: G > A 916 missense_variant 17: 39725756: G > A 925 synonymous_variant 17: 39725769: G > A 930 missense_variant 17: 39725797: A > G 939 missense_variant 17: 39725808: C > T 943 stop_gained 17: 39725825: C > A 948 synonymous_variant 17: 39725857: C > T splice_region_variant 17: 39726577: C > G 963 missense_variant 17: 39726594: T > C 969 missense_variant 17: 39726596: C > G 969 missense_variant 17: 39726633: G > T 982 missense_variant 17: 39726651: G > A 988 missense_variant 17: 39726821: G > A 993 missense_variant 17: 39726829: C > T 995 synonymous_variant 17: 39726881: G > C 1013 missense_variant 17: 39726933: T > G 1030 missense_variant 17: 39726941: C > 1033 frameshift_variant 17: 39726941: C > G 1033 missense_variant 17: 39726958: C > T 1038 synonymous_variant 17: 39726959: G > A 1039 missense_variant 17: 39726980: A > T 1046 missense_variant 17: 39726987: G > A 1048 missense_variant 17: 39727294: G > A splice_acceptor_variant 17: 39727294: G > C splice_acceptor_variant 17: 39727305: G > T 1057 missense_variant 17: 39727308: A > C 1058 missense_variant 17: 39727344: C > T 1070 missense_variant 17: 39727366: C > T 1077 synonymous_variant 17: 39727370: G > A 1079 missense_variant 17: 39727373: G > T 1080 missense_variant 17: 39727450: C > 1105 frameshift_variant 17: 39727466: C > T 1111 missense_variant 17: 39727475: G > C 1114 missense_variant 17: 39727492: C > T 1119 synonymous_variant 17: 39727533: G > C 1133 missense_variant 17: 39727542: A > G 1136 missense_variant 17: 39727728: C > T 1151 missense_variant 17: 39727732: C > T 1152 synonymous_variant 17: 39727752: C > T 1159 missense_variant 17: 39727755: C > T 1160 missense_variant 17: 39727805: A > G 1177 missense_variant 17: 39727825: C > T 1183 synonymous_variant 17: 39727838: G > A 1188 missense_variant 17: 39727839: G > A 1188 missense_variant 17: 39727846: C > T 1190 synonymous_variant 17: 39727867: G > C 1197 missense_variant 17: 39727871: C > A 1199 missense_variant 17: 39727892: C > T 1206 stop_gained 17: 39727898: C > T 1208 missense_variant 17: 39727904: C > T 1210 missense_variant 17: 39727965: G > 1230 frameshift_variant 17: 39727974: C > T 1233 missense_variant 17: 39727976: C > T 1234 missense_variant 17: 39728002: G > A 1242 synonymous_variant 17: 39728006: G > C 1244 missense_variant 17: 39728023: G > C 1249 synonymous_variant 17: 39728032: C > T 1252 synonymous_variant

[0664] In some embodiments, the HER2+ cancer is a HER2+ breast cancer, gastric cancer, gastroesophageal (GEJ) cancer, or lung cancer.

[0665] In some embodiments, the breast cancer is in situ (noninvasive). In some embodiments, the breast cancer is invasive. In some embodiments, the breast cancer is metastatic.

[0666] In some embodiments, the breast cancer is a carcinoma (e.g., an adenocarcinoma). In some embodiments, the breast cancer is ductal carcinoma. In some embodiments, the breast cancer is invasive lobular carcinoma. In some embodiments, the breast cancer is inflammatory breast cancer. In some embodiments, the breast cancer is Paget's disease of the breast. In some embodiments, the breast cancer is phyllodes tumor.

[0667] In some embodiments, the gastric cancer is an adenocarcinoma. In some embodiments, the gastric cancer is an intestinal adenocarcinoma. In some embodiments, the gastric cancer is a diffuse adenocarcinoma. In some embodiments, the gastric cancer is a lymphoma. In some cases, the gastric cancer is a mucosa-associated lymphoid tissue lymphoma. In some embodiments, the gastric cancer is a gastrointestinal stromal tumor. In some embodiments, the gastric cancer is a gastric carcinoid tumor. In some embodiments, the gastric cancer is a Type I, Type II, or Type III ECL-cell carcinoid. In some embodiments, the gastric cancer is a hereditary diffuse gastric cancer.

[0668] In some embodiments, the GEJ cancer is a GEJ adenocarcinoma.

[0669] In some embodiments, the lung cancer is non-small cell lung cancer (NSCLC), e.g., metastatic NSCLC. In some embodiments, the patient has a NSCLC with a HER2 activating mutation.

[0670] In some embodiments, the patient is refractory to or has a recurrence of after treatment, e.g., with trastuzumab or a biosimilar thereof.

[0671] In some embodiments, the patient is refractory to or has a recurrence after receiving 1 or more prior systemic therapies. In some embodiments, the patient is refractory to or has a recurrence after receiving 2 or more prior systemic therapies.

[0672] In some embodiments, when the patient has been diagnosed with a IHC 3+ or IHC 2+/ISH+ cancer, according to ASCOR Guidelines, e.g., the 2018 ASCOR Guidelines, e.g., as described in Wolff et al., Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med 142:1364-82 (2018), which is hereby incorporated by reference in its entirety, the patient is refractory to or has a recurrence after receiving previous treatment with a HER2-targeting therapy.

[0673] In some embodiments, when the patient has been diagnosed with a HER2 expressing cancer (e.g., breast cancer) that is IHC 1+ or IHC 2+/ISH, according to ASCOR Guidelines, e.g., the 2018 ASCOR Guidelines, e.g., as described in Wolff et al., Human Epidermal Growth Factor Receptor 2 Testing in Breast Cancer, Arch Pathol Lab Med 142:1364-82 (2018), which is hereby incorporated by reference in its entirety, the patient's cancer is unresectable, metastatic, or the patient is refractory to or has a recurrence after receiving previous treatment.

[0674] In some embodiments, the patient is refractory to or has a recurrence after treatment with pertuzumab (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof) and docetaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the pertuzumab (or FDA-approved biosimilar thereof) is administered at 840 mg IV day I followed by 420 mg IV. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 7 mg/kg IV day I followed by 6 mg/kg IV day 1 every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the docetaxel (or pharmaceutically acceptable salt thereof) is administered at 75-100 mg/m2 IV day 1 cycled every 21 days.

[0675] In some embodiments, the patient is refractory to or has a recurrence after treatment with pertuzumab (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof), and paclitaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the pertuzumab (or FDA-approved biosimilar thereof) is administered at 840 mg IV day I followed by 420 mg IV, cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the paclitaxel (or pharmaceutically acceptable salt thereof) is administered at 80 mg/m2 IV day 1 weekly or 175 mg/m2 day 1 cycled every 21 days.

[0676] In some embodiments, the patient is refractory to or has a recurrence after treatment with tucatinib (or pharmaceutically acceptable salt thereof), trastuzumab (or FDA-approved biosimilar thereof), and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the tucatinib (or FDA-approved biosimilar thereof) is administered at 300 mg orally twice daily on days 1-21. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 8 mg/kg IV day I followed by 6 mg/kg IV day 1 every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the capecitabine (or FDA-approved biosimilar thereof) is administered at 1000 mg/m2 orally twice daily on days 1-14. In some embodiments, the administration of tucatinib (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof), and capecitabine (or pharmaceutically acceptable salt thereof) is cycled every 21 days.

[0677] In some embodiments, the patient is refractory to or has a recurrence after treatment with ado-trastuzumab emtansine (T-DM1) (or FDA-approved biosimilar thereof). In some embodiments, the ado-trastuzumab emtansine (T-DM1) (or FDA-approved biosimilar thereof) is administered at 3.6 mg/kg IV day 1, cycled every 21 days.

[0678] In some embodiments, the patient is refractory to or has a recurrence after treatment with fam-trastuzumab deruxtecan-nxki (or FDA-approved biosimilar thereof). In some embodiments, the fam-trastuzumab deruxtecan-nxki (or FDA-approved biosimilar thereof) is administered at 5.4 mg/kg IV day 1, cycled every 21 days.

[0679] In some embodiments, the patient is refractory to or has a recurrence after treatment with paclitaxel/carboplatin (or pharmaceutically acceptable salts thereof) and trastuzumab (or FDA-approved biosimilar thereof). In some embodiments, the carboplatin/paclitaxel (or pharmaceutically acceptable salts thereof) is administered at AUC 6 IV day 1 carboplatin and 175 mg/m2 IV day 1 paclitaxel), cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0680] In some embodiments, the patient is refractory to or has a recurrence after treatment with paclitaxel/carboplatin (or pharmaceutically acceptable salts thereof) and trastuzumab (or FDA-approved biosimilar thereof). In some embodiments, the carboplatin/paclitaxel (or pharmaceutically acceptable salts thereof) is administered at AUC 2 IV carboplatin and 80 mg/m2 IV day I paclitaxel), days 1, 8, and 15, cycled every 28 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0681] In some embodiments, the patient is refractory to or has a recurrence after treatment with trastuzumab (or FDA-approved biosimilar thereof) and paclitaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the paclitaxel (or pharmaceutically acceptable salt thereof) is administered at 175 mg/m2 IV day 1 cycled every 21 days or 80-90 mg/m2 IV day 1 weekly. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day 1 every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0682] In some embodiments, the patient is refractory to or has a recurrence after treatment with trastuzumab (or FDA-approved biosimilar thereof) and docetaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the docetaxel (or pharmaceutically acceptable salt thereof) is administered at 80-100 mg/m2 IV day 1 cycled every 21 days or 35 mg/m2 IV days 1, 8, and 15 weekly. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day 1 followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0683] In some embodiments, the patient is refractory to or has a recurrence after treatment with trastuzumab (or FDA-approved biosimilar thereof) and vinorelbine (or pharmaceutically acceptable salt thereof). In some embodiments, the vinorelbine (or pharmaceutically acceptable salt thereof) is administered at 25 mg/m2 IV day I weekly or 20-35 mg/m2 IV days I and 8, cycled every 21 days, or 25-30 mg/m2 IV days 1, 8, and 15, cycled every 28 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0684] In some embodiments, the patient is refractory to or has a recurrence after treatment with trastuzumab (or FDA-approved biosimilar thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the capecitabine (or pharmaceutically acceptable salt thereof) is administered at 1000-1250 mg/m2 PO twice daily days 1-14 cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day 1 every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0685] In some embodiments, the patient is refractory to or has a recurrence after treatment with lapatinib (or pharmaceutically acceptable salt thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the lapatinib (or pharmaceutically acceptable salt thereof) is administered at 1250 mg/m2 PO daily days 1-21. In some embodiments, the capecitabine (or pharmaceutically acceptable salt thereof) is administered at 1000 mg/m2 PO twice daily days 1-14, cycled every 21 days.

[0686] In some embodiments, the patient is refractory to or has a recurrence after treatment with trastuzumab (or FDA-approved biosimilar thereof) and lapatinib (or pharmaceutically acceptable salt thereof). In some embodiments, the administered (or pharmaceutically acceptable salt thereof) is administered at 1000 mg/m2 PO daily. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0687] In some embodiments, the patient is refractory to or has a recurrence after treatment with neratinib (or pharmaceutically acceptable salt thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the neratinib is administered at 240 mg/m2 PO daily on days 1-21. Ins ome embodiments, the capecitabine is administered at 750 mg/m2 PO twice daily on days 1-14, cycled every 21 days.

[0688] In some embodiments, the patient has an oxygen saturation level measured via pulse oximeter of at least 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% within 1, 5, 10, 15, 20. 30, 45, 60, or 90 minutes, or within 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 18 hours, or within 1 day, 1 week, or 1 month of treatment.

[0689] In some embodiments, the patient has a left ventricular ejection fraction (LVEF) of at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, or 65%, measured within 1 day, 1 week, or 1 month of treatment.

[0690] In some embodiments, the patient has an absolute neutrophil count (ANC) of at least 1000/mm.sup.3 (1.0109/L), 1500/mm.sup.3, 2000/mm.sup.3, 2500/mm.sup.3, 3000/mm.sup.3, 3500/mm.sup.3, 4000/mm.sup.3, 4500/mm.sup.3. 5000/mm.sup.3, 5500/mm.sup.3, or 6000/mm.sup.3, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient has an ANC of 1000/mm.sup.3 to 6000/mm.sup.3, 1000/mm.sup.3 to 5500/mm.sup.3, 1000/mm.sup.3 to 5000/mm.sup.3, 1000/mm.sup.3 to 4500/mm.sup.3, 1000/mm.sup.3 to 4000/mm.sup.3, 1000/mm.sup.3 to 3500/mm.sup.3, 1000/mm.sup.3 to 3000/mm.sup.3, 1000/mm.sup.3 to 2500/mm.sup.3. 1000/mm.sup.3 to 2000/mm.sup.3, 1000/mm.sup.3 to 1500/mm.sup.3, 1500/mm.sup.3 to 6000/mm.sup.3, 1500/mm.sup.3 to 5500/mm.sup.3, 1500/mm.sup.3 to 5000/mm.sup.3, 1500/mm.sup.3 to 4500/mm.sup.3, 1500/mm.sup.3 to 4000/mm.sup.3. 1500/mm.sup.3 to 3500/mm.sup.3. 1500/mm.sup.3 to 3000/mm.sup.3. 1500/mm.sup.3 to 2500/mm.sup.3. 1500/mm.sup.3 to 2000/mm.sup.3. 2000/mm.sup.3 to 6000/mm.sup.3, 2000/mm.sup.3 to 5500/mm.sup.3, 2000/mm.sup.3 to 5000/mm.sup.3. 2000/mm.sup.3 to 4500/mm.sup.3, 2000/mm.sup.3 to 4000/mm.sup.3, 2000/mm.sup.3 to 3500/mm.sup.3, 2000/mm.sup.3 to 3000/mm.sup.3. 2000/mm.sup.3 to 2500/mm.sup.3, 2500/mm.sup.3 to 6000/mm.sup.3, 2500/mm.sup.3 to 5500/mm.sup.3. 2500/mm.sup.3 to 5000/mm.sup.3, 2500/mm.sup.3 to 4500/mm.sup.3, 2500/mm.sup.3 to 4000/mm.sup.3, 2500/mm.sup.3 to 3500/mm.sup.3, 2500/mm.sup.3 to 3000/mm.sup.3, 3000/mm.sup.3 to 6000/mm.sup.3, 3000/mm.sup.3 to 5500/mm.sup.3. 3000/mm.sup.3 to 5000/mm.sup.3, 3000/mm.sup.3 to 4500/mm.sup.3, 3000/mm.sup.3 to 4000/mm.sup.3, 3000/mm.sup.3 to 3500/mm.sup.3. 3500/mm.sup.3 to 6000/mm.sup.3, 3500/mm.sup.3 to 5500/mm.sup.3, 3500/mm.sup.3 to 5000/mm.sup.3.

[0691] 3500/mm.sup.3 to 4500/mm.sup.3, 3500/mm.sup.3 to 4000/mm.sup.3, 4000/mm.sup.3 to 6000/mm.sup.3, 4000/mm.sup.3 to 5500/mm.sup.3, 4000/mm.sup.3 to 5000/mm.sup.3, 4000/mm.sup.3 to 4500/mm.sup.3, 4500/mm.sup.3 to 6000/mm.sup.3. 4500/mm.sup.3 to 5500/mm.sup.3, 4500/mm.sup.3 to 5000/mm.sup.3, 5000/mm.sup.3 to 6000/mm.sup.3, 5000/mm.sup.3 to 5500/mm.sup.3, or 5500/mm.sup.3 to 6000/mm.sup.3, measured within 1 day, 1 week, or 1 month of treatment.

[0692] In some embodiments, the patient has a platelet count of at least 75000/mm.sup.3 (75109/L), 100000/mm.sup.3, 125000/mm.sup.3, 150000/mm.sup.3, 175000/mm.sup.3, 200000/mm.sup.3, 225000/mm.sup.3, 250000/mm.sup.3, 275000/mm.sup.3, 300000/mm.sup.3, 325000/mm.sup.3, 350000/mm.sup.3, 375000/mm.sup.3, or 400000/mm.sup.3, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient has a platelet count of 75000/mm.sup.3 to 400000/mm.sup.3, 75000/mm.sup.3 to 375000/mm.sup.3, 75000/mm.sup.3 to 350000/mm.sup.3, 75000/mm.sup.3 to 300000/mm.sup.3, 75000/mm.sup.3 to 275000/mm.sup.3, 75000/mm.sup.3 to 250000/mm.sup.3, 75000/mm.sup.3 to 225000/mm.sup.3, 75000/mm.sup.3 to 200000/mm.sup.3, 75000/mm.sup.3 to 175000/mm.sup.3, 75000/mm.sup.3 to 150000/mm.sup.3, 75000/mm.sup.3 to 125000/mm.sup.3, 75000/mm.sup.3 to 100000/mm.sup.3, 100000/mm.sup.3 to 400000/mm.sup.3, 100000/mm.sup.3 to 375000/mm.sup.3, 100000/mm.sup.3 to 350000/mm.sup.3. 100000/mm.sup.3 to 325000/mm.sup.3. 100000/mm.sup.3 to 300000/mm.sup.3.

[0693] 100000/mm.sup.3 to 275000/mm.sup.3, 100000/mm.sup.3 to 250000/mm.sup.3, 100000/mm.sup.3 to 225000/mm.sup.3, 100000/mm.sup.3 to 200000/mm.sup.3, 100000/mm.sup.3 to 175000/mm.sup.3, 100000/mm.sup.3 to 150000/mm.sup.3, 100000/mm.sup.3 to 125000/mm.sup.3, 125000/mm.sup.3 to 400000/mm.sup.3, 125000/mm.sup.3 to 375000/mm.sup.3, 125000/mm.sup.3 to 350000/mm.sup.3, 125000/mm.sup.3 to 325000/mm.sup.3, 125000/mm.sup.3 to 300000/mm.sup.3, 125000/mm.sup.3 to 275000/mm.sup.3, 125000/mm.sup.3 to 250000/mm.sup.3, 125000/mm.sup.3 to 225000/mm.sup.3, 125000/mm.sup.3 to 200000/mm.sup.3, 125000/mm.sup.3 to 175000/mm.sup.3, 125000/mm.sup.3 to 150000/mm.sup.3, 150000/mm.sup.3 to 400000/mm.sup.3, 150000/mm.sup.3 to 375000/mm.sup.3, 150000/mm.sup.3 to 350000/mm.sup.3, 150000/mm.sup.3 to 325000/mm.sup.3, 150000/mm.sup.3 to 300000/mm.sup.3, 150000/mm.sup.3 to 275000/mm.sup.3, 150000/mm.sup.3 to 250000/mm.sup.3, 150000/mm.sup.3 to 225000/mm.sup.3, 150000/mm.sup.3 to 200000/mm.sup.3, 150000/mm.sup.3 to 175000/mm.sup.3. 175000/mm.sup.3 to 400000/mm.sup.3. 175000/mm.sup.3 to 375000/mm.sup.3. 175000/mm.sup.3 to 350000/mm.sup.3, 175000/mm.sup.3 to 325000/mm.sup.3, 175000/mm.sup.3 to 300000/mm.sup.3, 175000/mm.sup.3 to 275000/mm.sup.3, 175000/mm.sup.3 to 250000/mm.sup.3, 175000/mm.sup.3 to 225000/mm.sup.3, 175000/mm.sup.3 to 200000/mm.sup.3, 200000/mm.sup.3 to 400000/mm.sup.3, 200000/mm.sup.3 to 375000/mm.sup.3, 200000/mm.sup.3 to 350000/mm.sup.3, 200000/mm.sup.3 to 325000/mm.sup.3, 200000/mm.sup.3 to 300000/mm.sup.3, 200000/mm.sup.3 to 275000/mm.sup.3, 200000/mm.sup.3 to 250000/mm.sup.3, 200000/mm.sup.3 to 225000/mm.sup.3, 225000/mm.sup.3 to 400000/mm.sup.3, 225000/mm.sup.3 to 375000/mm.sup.3, 225000/mm.sup.3 to 350000/mm.sup.3, 225000/mm.sup.3 to 325000/mm.sup.3, 225000/mm.sup.3 to 300000/mm.sup.3, 225000/mm.sup.3 to 275000/mm.sup.3, 225000/mm.sup.3 to 250000/mm.sup.3, 250000/mm.sup.3 to 400000/mm.sup.3, 250000/mm.sup.3 to 375000/mm.sup.3, 250000/mm.sup.3 to 350000/mm.sup.3, 275000/mm.sup.3 to 325000/mm.sup.3, 275000/mm.sup.3 to 300000/mm.sup.3, 300000/mm.sup.3 to 400000/mm.sup.3, 300000/mm.sup.3 to 375000/mm.sup.3, 300000/mm.sup.3 to 350000/mm.sup.3, 300000/mm.sup.3 to 325000/mm.sup.3, 325000/mm.sup.3 to 400000/mm.sup.3, 350000/mm.sup.3 to 400000/mm.sup.3, 350000/mm.sup.3 to 375000/mm.sup.3, or 375000/mm.sup.3 to 400000/mm.sup.3, measured within 1 day, 1 week, or 1 month of treatment.

[0694] In some embodiments, the patient's hemoglobin level, with or without prior transfusion, is at least 8.0, 9.0, 10.0, 11.0, 12.0, 130, 14.0, 15.0, 16.0, 17.0, 18.0, 19.0, or 20.0 g/dL, measured within 1 day. 1 week, or 1 month of treatment. In some embodiments, the patient's hemoglobin level, with or without prior transfusion, is 8.0 to 20.0, 8.0 to 19.0, 8.0 to 18.0, 8.0 to 17.0, 8.0 to 16.0, 8.0 to 15.0. 8.0 to 14.0, 8.0 to 13.0, 8.0 to 12.0, 8.0 to 11.0, 8.0 to 10.0, 8.0 to 9.0, 9.0 to 20.0, 9.0 to 19.0, 9.0 to 18.0, 9.0 to 17.0, 9.0 to 16.0, 9.0 to 15.0, 9.0 to 14.0, 9.0 to 13.0, 9.0 to 12.0, 9.0 to 11.0. 9.0 to 10.0, 10.0 to 20.0, 10.0 to 19.0, 10.0 to 18.0, 10.0 to 17.0, 10.0 to 16.0, 10.0 to 15.0, 10.0 to 14.0, 10.0 to 13.0, 10.0 to 12.0, 10.0 to 11.0, 11.0 to 20.0. 11.0 to 19.0, 11.0 to 18.0, 11.0 to 17.0. 11.0 to 16.0. 11.0 to 15.0. 11.0 to 14.0. 11.0 to 13.0. 11.0 to 12.0, 12.0 to 20.0, 12.0 to 19.0, 12.0 to 18.0, 12.0 to 17.0, 12.0 to 16.0, 12.0 to 15.0, 12.0 to 14.0, 12.0 to 13.0, 13.0 to 20.0, 13.0 to 19.0, 13.0 to 18.0, 13.0 to 17.0, 13.0 to 16.0, 13.0 to 15.0, 13.0 to 14.0, 14.0 to 20.0, 14.0 to 19.0. 14.0 to 18.0, 14.0 to 17.0, 14.0 to 16.0, 14.0 to 15.0, 15.0 to 20.0, 15.0 to 19.0, 15.0 to 18.0, 15.0 to 17.0, 15.0 to 16.0, 16.0 to 20.0, 16.0 to 19.0, 16.0 to 18.0, 16.0 to 17.0, 17.0 to 20.0, 17.0 to 19.0, 17.0 to 18.0, 18.0 to 20.0, 18.0 to 19.0, or 19.0 to 20.0, measured within 1 day, 1 week, or 1 month of treatment.

[0695] In some embodiments, the patient's creatinine clearance is at least 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, or 140 mL/min using the Cockcraft-Gault equation, or mL/min/1.73 m.sup.2 as estimated glomerular filtration rate (eGFR) per Modification of Diet in Renal Disease (MDRD) equation, measured within 1 day. I week, or] month of treatment. In some embodiments, the patient's creatinine clearance is 45 to 150, 45 to 125, 45 to 100, 45 to 75, 75 to 150, 75 to 125, 75 to 100, 100 to 150, 100 to 125, or 125 to 150 mL/min using the Cockcraft-Gault equation, or mL/min/1.73 m.sup.2 as estimated glomerular filtration rate (eGFR) per Modification of Diet in Renal Disease (MDRD) equation, measured within 1 day, 1 week, or 1 month of treatment.

[0696] In some embodiments, the patient's total serum bilirubin is less than 5, 4.5, 4.0, 3.5. 3.0, 2.5, 2.0, 1.5, 1.0, 0.5, 0.4, 0.3, 0.2, or 0.1 mg/dL, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient's total serum bilirubin is 0.1 to 5, 0.1 to 4.5, 0.1 to 4.0, 0.1 to 3.5, 0.1 to 3.0, 0.1 to 2.5, 0.1 to 2.0, 0.1 to 1.5, 0.1 to 0.5, 0.1 to 0.4, 0.1 to 0.3, 0.1 to 0.2, 0.2 to 5.0, 0.2 to 4.5, 0.2 to 4.0, 0.2 to 3.5, 0.2 to 3.0, 0.2 to 2.5, 0.2 to 2.0, 0.2 to 1.5, 0.2 to 1.0, 0.2 to 0.5, 0.2 to 0.4, 0.2 to 0.3, 0.3 to 5.0, 0.3 to 4.5, 0.3 to 4.0, 0.3 to 3.5, 0.3 to 3.0, 0.3 to 2.5, 0.3 to 2.0, 0.3 to 1.5, 0.3 to 1.0, 0.3 to 0.5, 0.3 to 0.4, 0.4 to 5.0, 0.4 to 4.5, 0.4 to 4.0, 0.4 to 3.5, 0.4 to 3.0, 0.4 to 2.5, 0.4 to 2.0, 0.4 to 1.5, 0.4 to 1.0, 0.4 to 0.5, 0.5 to 5.0, 0.5 to 4.5, 0.5 to 4.0, 0.5 to 3.5, 0.5 to 3.0, 0.5 to 2.5, 0.5 to 2.0, 0.5 to 1.5, 0.5 to 1.0, 1.0 to 5.0, 1.0 to 4.5, 1.0 to 4.0, 1.0 to 3.5, 1.0 to 3.0, 1.0 to 2.5, 1.0 to 2.0, 1.0 to 1.5, 1.5 to 5.0, 1.5 to 4.5, 1.5 to 4.0, 1.5 to 4.0, 1.5 to 3.5, 1.5 to 3.0, 1.5 to 2.5, 1.5 to 2.0, 2.0 to 5.0, 2.0 to 4.5, 2.0 to 4.0, 2.0 to 3.5, 2.0 to 3.0, 2.0 to 2.5, 2.5 to 5.0, 2.5 to 4.5, 2.5 to 4.0, 2.5 to 3.5, 2.5 to 3.0, 3.0 to 5.0, 3.0 to 4.5, 3.0 to 4.0, 3.5 to 5.0, 3.5 to 4.5, 3.5 to 4.0, 4.0 to 5.0, 4.0 to 4.5, 4.5 to 5.0 mg/dL, measured within 1 day, 1 week, or 1 month of treatment.

[0697] In some embodiments, when the patient has known Gilbert's Syndrome, the patient's total serum bilirubin is less than 5, 4.5, 4.0, 3.5, 3.0, 2.5. 2.0, 1.5, 1.0, 0.5, 0.4, 0.3, 0.2, or 0.1 mg/dL, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, when the patient has known Gilbert's Syndrome, the patient's total serum bilirubin is 0.1 to 5, 0.1 to 4.5, 0.1 to 4.0, 0.1 to 3.5, 0.1 to 3.0, 0.1 to 2.5, 0.1 to 2.0, 0.1 to 1.5, 0.1 to 0.5, 0.1 to 0.4. 0.1 to 0.3, 0.1 to 0.2, 0.2 to 5.0, 0.2 to 4.5, 0.2 to 4.0, 0.2 to 3.5, 0.2 to 3.0, 0.2 to 2.5, 0.2 to 2.0. 0.2 to 1.5, 0.2 to 1.0, 0.2 to 0.5, 0.2 to 0.4, 0.2 to 0.3, 0.3 to 5.0, 0.3 to 4.5, 0.3 to 4.0, 0.3 to 3.5. 0.3 to 3.0, 0.3 to 2.5, 0.3 to 2.0, 0.3 to 1.5, 0.3 to 1.0, 0.3 to 0.5, 0.3 to 0.4, 0.4 to 5.0, 0.4 to 4.5. 0.4 to 4.0, 0.4 to 3.5, 0.4 to 3.0, 0.4 to 2.5, 0.4 to 2.0, 0.4 to 1.5, 0.4 to 1.0, 0.4 to 0.5, 0.5 to 5.0, 0.5 to 4.5, 0.5 to 4.0, 0.5 to 3.5, 0.5 to 3.0, 0.5 to 2.5, 0.5 to 2.0, 0.5 to 1.5, 0.5 to 1.0, 1.0 to 5.0. 1.0 to 4.5, 1.0 to 4.0, 1.0 to 3.5, 1.0 to 3.0, 1.0 to 2.5, 1.0 to 2.0, 1.0 to 1.5, 1.5 to 5.0, 1.5 to 4.5. 1.5 to 4.0, 1.5 to 4.0, 1.5 to 3.5, 1.5 to 3.0, 1.5 to 2.5, 1.5 to 2.0, 2.0 to 5.0, 2.0 to 4.5, 2.0 to 4.0. 2.0 to 3.5. 2.0 to 3.0. 2.0 to 2.5. 2.5 to 5.0, 2.5 to 4.5. 2.5 to 4.0, 2.5 to 3.5. 2.5 to 3.0, 3.0 to 5.0, 3.0 to 4.5, 3.0 to 4.0, 3.5 to 5.0, 3.5 to 4.5, 3.5 to 4.0, 4.0 to 5.0, 4.0 to 4.5, 4.5 to 5.0 mg/dL, measured within 1 day, 1 week, or 1 month of treatment.

[0698] In some embodiments, when the patient does not have known Gilbert's Syndrome, the patient's total serum bilirubin is less than 2.5, 2.0, 1.5, 1.0, 0.5, 0.4, 0.3, 0.2, or 0.1 mg/dL, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, when the patient does not have known Gilbert's Syndrome, the patient's total serum bilirubin is 0.1 to 2.5, 0.1 to 2.0, 0.1 to 1.5, 0.1 to 1.0, 0.1 to 0.5, 0.1 to 0.4, 0.1 to 0.3, 0.1 to 0.2, 0.2 to 2.5, 0.2 to 2.0, 0.2 to 1.5, 0.2 to 1.0, 0.2 to 0.5, 0.2 to 0.4, 0.2 to 0.3, 0.3 to 2.5, 0.3 to 2.0, 0.3 to 1.5, 0.3 to 1.0, 0.3 to 0.5, 0.3 to 0.4, 0.4 to 2.5, 0.4 to 2.0, 0.4 to 1.5, 0.4 to 1.0, 0.4 to 0.5, 0.5 to 2.5, 0.5 to 2.0, 0.5 to 1.5, 0.5 to 1.0, 1.0 to 2.5, 1.0 to 2.0, 1.0 to 1.5, 1.5 to 2.5, 1.5 to 2.0, or 2.0 to 2.5 mg/dL, measured within 1 day, 1 week, or 1 month of treatment.

[0699] In some embodiments, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is less than or equal to 5, 4.5, 4.0, 3.5, 3.0, 2.5, 2.0, 1.5, 1.0, 0.5, or 0 times the upper limit of normal (ULN) for the test, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is below the upper limit of normal to 5.0 times the upper limit of normal for the test, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is 0 to 5.0, 0 to 4.5, 0 to 4.0, 0 to 3.5, 0 to 3.0, 0 to 2.5, 0 to 2.0, 0 to 1.5, 0 to 1.0, 0 to 0.5, 0.5 to 5.0, 0.5 to 4.5, 0.5 to 4.0, 0.5 o 3.5. 0.5 to 3.0, 0.5 to 2.5, 0.5 to 2.0. 0.5 to 1.5. 0.5 to 1.0. 1.0 to 5.0, 1.0 to 4.5. 1.0 to 4.0, 1.0 to 3.5. 1.0 to 3.0, 1.0 to 2.5. 1.0 to 2.0, 1.0 to 1.5, 1.5 to 5.0, 1.5 to 4.5, 1.5 to 4.0, 1.5 to 3.5, 1.5 to 3.0, 1.5 to 2.5, 1.5 to 2.0. 2.0 to 5.0, 2.0 to 4.5, 2.0 to 4.0, 2.0 to 3.5, 2.0 to 3.0, 2.0 to 2.5, 2.5 to 5.0, 2.5, to 4.5, 2.5 to 4.0, 2.5 to 3.5, 2.5 to 3.0, 3.0 to 5.0, 3.0 to 4.5, 3.0 to 4.0, 3.0 to 3.5, 3.5 to 5.0, 3.5 to 4.5, 3.5 to 4.0, 4.0 to 5.0, 4.0 to 4.5, or 4.5 to 5.0 times the upper limit of normal for the test, measured within 1 day, 1 week, or 1 month of treatment.

[0700] In some embodiments, when the patient has liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is less than or equal to 5, 4.5, 4.0, 3.5, 3.0, 2.5, 2.0, 1.5, 1.0, 0.5, or 0 times the upper limit of normal (ULN) for the test, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, when the patient has liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is below the upper limit of normal to 5.0 times the upper limit of normal for the test, measured within 1 day, I week, or 1 month of treatment. In some embodiments, when the patient has liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is 0 to 5.0, 0 to 4.5, 0 to 4.0, 0 to 3.5, 0 to 3.0, 0 to 2.5, 0 to 2.0, 0 to 1.5, 0 to 1.0, 0 to 0.5, 0.5 to 5.0, 0.5 to 4.5, 0.5 to 4.0, 0.5 3.5, 0.5 to 3.0, 0.5 to 2.5, 0.5 to 2.0, 0.5 to 1.5, 0.5 to 1.0, 1.0 to 5.0, 1.0 to 4.5, 1.0 to 4.0, 1.0 to 3.5, 1.0 to 3.0, 1.0 to 2.5, 1.0 to 2.0, 1.0 to 1.5, 1.5 to 5.0, 1.5 to 4.5, 1.5 to 4.0, 1.5 to 3.5, 1.5 to 3.0, 1.5 to 2.5, 1.5 to 2.0, 2.0 to 5.0, 2.0 to 4.5, 2.0 to 4.0, 2.0 to 3.5, 2.0 to 3.0, 2.0 to 2.5, 2.5 to 5.0, 2.5, to 4.5. 2.5 to 4.0, 2.5 to 3.5, 2.5 to 3.0, 3.0 to 5.0, 3.0 to 4.5, 3.0 to 4.0, 3.0 to 3.5, 3.5 to 5.0, 3.5 to 4.5, 3.5 to 4.0, 4.0 to 5.0, 4.0 to 4.5, or 4.5 to 5.0 times the upper limit of normal for the test, measured within 1 day, 1 week, or 1 month of treatment.

[0701] In some embodiments, when the patient does not have liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is less than or equal to 2.5, 2.0, 1.5, 1.0, 0.5, or 0 times the upper limit of normal (ULN) for the test, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, when the patient does not have liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is below the upper limit of normal to 5.0 times the upper limit of normal for the test, measured within 1 day, 1 week, or 1 month of treatment. In some embodiments, when the patient does not have liver metastases, the patient's liver transaminases (aspartate aminotransferase/alanine amino transferase/alkaline phosphatase (AST/ALT/ALP)) is 0 to 2.5. 0 to 2.0, 0 to 1.5. 0 to 1.0.

[0702] 0 to 0.5, 0.5 to 2.5, 0.5 to 2.0, 0.5 to 1.5, 0.5 to 1.0, 1.0 to 2.5, 1.0 to 2.0, 1.0 to 1.5, 1.5 to 2.5, 1.5 to 2.0, or 2.0 to 2.5 times the upper limit of normal for the test, measured within 1 day, 1 week, or 1 month of treatment.

[0703] In some embodiments, the patient does not have active nervous system metastases. In some embodiments, the patient does have active nervous system metastases.

[0704] In some embodiments, the patient does not have a history or presence of a clinically relevant CNS disorder such as a seizure disorder (e.g., epilepsy), cerebrovascular ischemia/hemorrhage, dementia, cerebellar disease, cerebral edema, posterior reversible encephalopathy syndrome (PRES), or any autoimmune disease with CNS involvement.

[0705] In some embodiments, the patient does not have active human immunodeficiency virus (HIV) infection, e.g., as evidenced by a positive HIV polymerase chain reaction (PCR) test, e.g., within 1 day, 1 week, or 1 month of treatment.

[0706] In some embodiments, the patient does not have active HBV or hepatitis C virus (HCV) infection, e.g., based on laboratory testing, e.g., within 1 day, 1 week, or 1 month of treatment. In some embodiments, the patient has a viral HBV load below the institutional limit of quantification (LOQ) and the subject is on stable viral suppressive therapy. In some embodiments, the patient has a HCV RNA viral load below the institutional LOQ and has completed curative antiviral treatment.

Lymphodepletion

[0707] In some embodiments, the patient is lymphodepleted before treatment.

[0708] Illustrative lymphodepleting chemotherapy regimens, along with correlative beneficial biomarkers, are described in WO 2016/191756 and WO 2019/079564, hereby incorporated by reference in their entirety. In certain embodiments, the lymphodepleting chemotherapy regimen comprises administering to the patient doses of cyclophosphamide (between 200 mg/m.sup.2/day and 2000 mg/m.sup.2/day) and doses of fludarabine (between 20 mg/m.sup.2/day and 900 mg/m.sup.2/day).

[0709] In some embodiments, lymphodepletion comprises administration of or of about 250 to about 500 mg/m.sup.2 of cyclophosphamide, e.g., from or from about 250 to or to about 500, 250, 400, 500, about 250, about 400, or about 500 mg/m.sup.2 of cyclophosphamide.

[0710] In some embodiments, lymphodepletion comprises administration of or of about 20 mg/m.sup.2/day to or to about 40 mg/m.sup.2/day fludarabine, e.g., 30 or about 30 mg/m.sup.2/day.

[0711] In some embodiments, lymphodepletion comprises administration of both cyclophosmamide and fludarabine.

[0712] In some embodiments, the patient is lymphodepleted by intravenous administration of cyclophosphamide (250 mg/m.sup.2/day) and fludarabine (30 mg/m.sup.2/day).

[0713] In some embodiments, the patient is lymphodepleted by intravenous administration of cyclophosphamide (500 mg/m.sup.2/day) and fludarabine (30 mg/m.sup.2/day).

[0714] In some embodiments, the lymphodepletion occurs no more than 5 days prior to the first dose of NK cells. In some embodiments, the lymphodepletion occurs no more than 7 days prior to the first dose of NK cells.

[0715] In some embodiments, lymphodepletion occurs daily for 3 consecutive days, starting 5 days before the first dose of NK cells (i.e., from Day-5 through Day-3).

[0716] In some embodiments, the lymphodepletion occurs on day-5, day-4 and day-3.

[0717] In some embodiments involving repeat or multiple doses, the patient is lymphodepleted before some or every dose as described above. In some embodiments, the patient is lymphodepleted two, three, four, five, or six times.

Administration

NK Cells

[0718] In some embodiments, the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein are administered to the patient as part of a pharmaceutical composition, e.g., a pharmaceutical composition described herein. Cells are administered after thawing, in some cases without any further manipulation in cases where their cryoprotectant is compatible for immediate administration. For a given individual, a treatment regimen often comprises administration over time of multiple aliquots or doses of NK cells, including from doses drawn from a common batch or donor.

[0719] The NK cells can be administered by any suitable means, for example, by bolus infusion, by injection, e.g., intravenous or subcutaneous injections, intraocular injection, periocular injection, subretinal injection, intravitreal injection, trans-septal injection, subscleral injection, intrachoroidal injection, intracameral injection, subconjectval injection, subconjuntival injection, sub-Tenon's injection, retrobulbar injection, peribulbar injection, or posterior juxtascleral delivery. In some embodiments, they are administered by parenteral, intrapulmonary, and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, or subcutaneous administration. In some embodiments, a given dose is administered by a single bolus administration of the cells. In some embodiments, a given dose is administered by multiple bolus administrations of the cells, for example, over a period of no more than 3 days, or by continuous infusion administration of the cells. In some embodiments, administration of the cell dose or any additional therapies, e.g., the lymphodepleting therapy, intervention therapy and/or combination therapy, is carried out via outpatient delivery.

[0720] In the context of adoptive cell therapy, administration of a given dose can encompass administration of the given amount or number of cells as a single composition and/or single uninterrupted administration, e.g., as a single injection or continuous infusion. A given dose can also encompass administration of the given amount or number of cells as a split dose or as a plurality of compositions, provided in multiple individual compositions or infusions, over a specified period of time, such as over no more than 3 days. Thus, in some contexts, the dose is a single or continuous administration of the specified number of cells, given or initiated at a single point in time. In some contexts, however, the dose is administered in multiple injections or infusions over a period of no more than three days, such as once a day for three days or for two day's or by multiple infusions over a single day period.

[0721] In some embodiments, the NK cells are administered to the patient at a range of at or about 1 million to at or about 100 billion cells. In some embodiments, the NK cells, e.g., the NK cells described herein, e.g., the CAR-NK cells described herein, are administered at or at about 510.sup.6 to or to about 110.sup.9 NK cells per dose. In some embodiments, the NK cells are administered at or at about 510.sup.6, at or at about 110.sup.7, at or at about 310.sup.7, at or at about 1108, at or at about 310.sup.8, or at or at about 110.sup.9 cells per dose. In some embodiments, the NK cells are administered at or about 1 million to at or about 20 billion cells (e.g., at or about 5 million cells, at or about 25 million cells, at or about 50 million cells, at or about 75 million cells, at or about 100 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, or a range defined by any two of the foregoing values), at or about 10 million to at or about 20 billion cells (e.g., at or about 25 million cells, at or about 50 million cells, at or about 75 million cells, at or about 100 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, at or about 20 billion cells, or a range defined by any two of the foregoing values), and in some cases at or about 100 million cells to at or about 50 billion cells (e.g., at or about 150 million cells, at or about 200 million cells, at or about 300 million cells, at or about 400 million cells, at or about 500 million cells, at or about 1 billion cells, at or about 2 billion cells, at or about 3 billion cells, at or about 4 billion cells, at or about 5 billion cells, at or about 6 billion cells, at or about 7 billion cells, at or about 8 billion cells, at or about 9 billion cells, at or about 10 billion cells, at or about 20 billion cells, at or about 30 billion cells, at or about 40 billion cells), or any value in between any of these ranges, per dose.

[0722] Thus, in some embodiments, the NK cells are administered in a dose comprising at or at about 110.sup.6, 210.sup.6, 310.sup.6. 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 cells per dose, at or at about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 510.sup.7, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 cells per dose, at or at about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 cells per dose, at or at about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 8109, or 910.sup.9 cells per dose, at or at about 110.sup.10 or 210.sup.10 cells per dose.

[0723] Thus, in some embodiments, the NK cells are administered in a dose comprising at least or at least about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6. 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 cells per dose, at least or at least about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 510.sup.7, 610.sup.7, 7107, 810.sup.7, or 910.sup.7 cells per dose, at least or at least about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 5108. 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 cells per dose, at least or at least about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 cells per dose, or at least or at least about 110.sup.10, or 210.sup.10 cells per dose.

[0724] In some embodiments, the NK cells are administered in a dose comprising at or about 1 million to at or about 20 billion CAR-expressing NK cells (e.g., at or about 5 million cells, at or about 25 million CAR-expressing cells, at or about 50 million CAR-expressing cells, at or about 75 million CAR-expressing cells, at or about 100 million CAR-expressing cells, at or about 200 million CAR-expressing cells, at or about 300 million CAR-expressing cells, at or about 400 million CAR-expressing cells, at or about 500 million CAR-expressing cells, at or about 1 billion CAR-expressing cells, at or about 2 billion CAR-expressing cells, at or about 3 billion CAR-expressing cells, at or about 4 billion CAR-expressing cells, at or about 5 billion CAR-expressing cells, at or about 6 billion CAR-expressing cells, at or about 7 billion CAR-expressing cells, at or about 8 billion CAR-expressing cells, at or about 9 billion CAR-expressing cells, at or about 10 billion CAR-expressing cells, or at or about 20 billion CAR-expressing cells, or a range defined by any two of the foregoing values), or any value in between any of these ranges, per dose.

[0725] Thus, in some embodiments, the NK cells are administered in a dose comprising at or at about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6, 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.7, 210.sup.7, 310.sup.7, 410.sup.7, 5107, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 CAR-expressing cells per dose, a dose comprising at or at about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 5109, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 CAR-expressing cells per dose, or a dose comprising at or at about 110.sup.10 or at or at about 210.sup.10 CAR-expressing cells per dose.

[0726] Thus, in some embodiments, the NK cells are administered in a dose comprising at least or at least about 110.sup.6, 210.sup.6, 310.sup.6, 410.sup.6. 510.sup.6, 610.sup.6, 710.sup.6, 810.sup.6, or 910.sup.6 CAR-expressing cells per dose, at least or at least about 110.sup.7, 210.sup.2, 310.sup.7, 410.sup.7, 5107, 610.sup.7, 710.sup.7, 810.sup.7, or 910.sup.7 CAR-expressing cells per dose, at least or at least about 110.sup.8, 210.sup.8, 310.sup.8, 410.sup.8, 510.sup.8, 610.sup.8, 710.sup.8, 810.sup.8, or 910.sup.8 CAR-expressing cells per dose, at least or at least about 110.sup.9, 210.sup.9, 310.sup.9, 410.sup.9, 510.sup.9, 610.sup.9, 710.sup.9, 810.sup.9, or 910.sup.9 CAR-expressing cells per dose, at least or at least about 110.sup.10 CAR-expressing cells per dose.

[0727] In some embodiments, the dose of cells is a flat dose of cells or fixed dose of cells such that the dose of cells is not tied to or based on the body surface area or weight of a patient.

[0728] In some embodiments, the dose of cells is administered based on the weight of the patient. For example, the dose can be determined per kilogram of body weight of the patient. In some embodiments, the dose of cells comprises between at or about 110.sup.5 of the cells/kg and at or about 110.sup.8 of the cells/kg, such as between at or about 1.510.sup.5 of the cells/kg and at or about 1.510.sup.7 of the cells/kg, or between at or about 410.sup.5 of the cells/kg and at or about 410.sup.6 of the cells/kg.

[0729] Thus, in some embodiments, the NK cells are administered in a dose comprising at or at about 110.sup.5, 1.510.sup.5, 210.sup.5, 2.510.sup.5, 310.sup.5, 3.510.sup.5, 410.sup.5, 4.510.sup.5, 510.sup.5, 5.5105, 610.sup.5, 6.510.sup.5, 710.sup.5, 7.510.sup.5, 810.sup.5, 8.510.sup.5, 910.sup.5, or 9.510.sup.5 cell/kg, 110.sup.6, 1.510.sup.6, 210.sup.6, 2.510.sup.6, 310.sup.6, 3.510.sup.6, 410.sup.6, 4.510.sup.6, 510.sup.6, 5.510.sup.6, 610.sup.6, 6.510.sup.6, 710.sup.6, 7.510.sup.6, 810.sup.6, 8.510.sup.6, 910.sup.6, or 9.510.sup.6 cells/kg, at or at about 110.sup.7, 1.510.sup.7, 210.sup.7, 2.510.sup.7, 310.sup.7, 3.510.sup.7, 410.sup.7, 4.510.sup.7, 510.sup.7, 5.510.sup.7, 610.sup.7, 6.510.sup.7, 710.sup.7, 7.510.sup.7, 810.sup.7, 8.510.sup.7, 910.sup.7 cells/kg, or 9.510.sup.7, or at or at about 110.sup.8, 1.510.sup.8, or 210.sup.8 cells/kg.

[0730] In some embodiments, the NK cells are administered in a dose comprising at least or at least about 110.sup.5. 1.510.sup.5, 210.sup.5, 2.510.sup.5, 310.sup.5, 3.510.sup.5, 410.sup.5, 4.510.sup.5. 510.sup.5, 5.510.sup.5, 610.sup.5, 6.510.sup.5, 710.sup.5, 7.510.sup.5, 810.sup.5, 8.510.sup.5, 910.sup.5, or 9.510.sup.5 cell/kg, at least or at least about 110.sup.6, 1.510.sup.6, 210.sup.6, 2.510.sup.6, 310.sup.6, 3.510.sup.6, 410.sup.6, 4.510.sup.6, 510.sup.6. 5.510.sup.6, 610.sup.6, 6.510.sup.6, 710.sup.6, 7.510.sup.6, 810.sup.6, 8.510.sup.6, 910.sup.6, or 9.510.sup.6cells/kg, at least or at least about 110.sup.7, 1.510.sup.7, 210.sup.7, 2.510.sup.7, 310.sup.7, 3.510.sup.7, 410.sup.7, 4.510.sup.7, 510.sup.7, 5.510.sup.7, 610.sup.7, 6.510.sup.7, 710.sup.7, 7.510.sup.7, 810.sup.7, 8.510.sup.7, 910.sup.7 cells/kg, or 9.510.sup.7, or at least or at least about 110.sup.8 or 1.510.sup.8 cells/kg.

[0731] In some embodiments, the dose of cells, e.g., recombinant receptor-expressing NK cells, is administered to the subject as a single dose or is administered only one time within a period of two weeks, one month, three months, six months, 1 year or more.

[0732] The ability to offer repeat dosing may allow patients to experience or maintain a deeper or prolonged response from the therapy. Thus, in some embodiments, the patient receives multiple doses, e.g., two or more doses or at least one subsequent dose, of the NK cells. In some embodiments, two, three, four, five, six, seven, eight, nine, or ten doses are administered to a subject. In some embodiments, the at least one subsequent dose comprises a second dose. In some embodiments, the at least one subsequent dose comprises a second dose and a third dose. In some embodiments, the at least one subsequent dose comprises a second, third, and fourth dose. In some embodiments, the at least one subsequent dose comprises a second, third, fourth, and fifth dose. In some embodiments, the at least one subsequent dose comprises a second, third, fourth, fifth, and sixth dose. In some embodiments, the at least one subsequent dose comprises a second, third, fourth, fifth, sixth, and seventh dose. In some embodiments, the at least one subsequent dose comprises a second, third, fourth, fifth, sixth, seventh, and eighth dose. In some embodiments, a patient can receive response-based dosing, during which the patient continues to receive doses of CAR-NK cell therapy for as long as the patient derives a benefit. The number of doses and the number of cells administered in each dose can also be tailored to the individual patient. In some embodiments, the number of cells administered to the subject in the additional or subsequent dose or doses are the same as or similar to the first dose. Thus, the CAR-NK cell therapies described herein can be tailored to each patient based on that patient's own response. In some cases, the therapy can be terminated if the patient no longer derives a benefit from the CAR-NK cell therapy. In some cases, the therapy can also be reinitiated if the patient relapses.

[0733] In some embodiments involving multiple doses or repeat dosing, the NK cells are administered weekly bi-weekly, once every three weeks, once every four weeks, once every five weeks, once every six weeks, once every seven weeks, once every eight weeks, once every nine weeks, once every ten weeks, once every 11 weeks, once every 12 weeks, once every 13 weeks, once every 14 weeks, once every 15 weeks, or once every 16 weeks. For example, a patient can receive a first dose, a second dose, and a third dose, wherein each dose is separated by or by about six weeks. In some embodiments, the NK cells are administered monthly. In some embodiments, the NK cells are administered every other month or once every three months. In some embodiments, the NK cells are administered for or for about 8 weeks.

[0734] In some embodiments, the dosing schedule can vary over the course of the therapy. Thus, for example, the patient can receive a first series of doses every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or 16 weeks and a second series of doses every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or 16 weeks, wherein the time between the first series of doses and the second series of doses is different. In some embodiments, the patient receives 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 doses in the first series of doses and 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 doses in the second series of doses, wherein the number of doses in the first series and the second series of doses can be the same or different. Thus, for example, a patient may receive four doses administered every other week in the first series of doses and four doses administered every 12 weeks in the second series of doses.

[0735] In some embodiments, the NK cells are administered between one and four times over the course of nine months.

[0736] In some embodiments, the NK cells are cryopreserved in an infusion-ready media. e.g., a cryopreservation composition suitable for intravenous administration, e.g., as described herein.

[0737] In some embodiments, the NK cells are cryopreserved in vials containing from or from about 110.sup.7 to or to about 110.sup.9 cells per vial. In some embodiments, the NK cells are cryopreserved in vials containing a single dose.

[0738] In some embodiments, the cells are thawed, e.g., in a 37 C., water bath, prior to administration.

[0739] In some embodiments, the thawed vial(s) of NK cells are aseptically transferred to a single administration vessel, e.g., administration bag using, e.g., a vial adapter and a sterile syringe. The NK cells can be administered to the patient from the vessel through a Y-type blood/solution set filter as an IV infusion, by gravity.

[0740] In some embodiments, the NK cells are administered as soon as practical, preferably less than 90 minutes, e.g., less than 80, 70, 60, 50, 40, 30, 20, or 10 minutes after thawing. In some embodiments, the NK cells are administered within 30 minutes of thawing.

[0741] In some embodiments, the pharmaceutical composition is administered intravenously via syringe.

[0742] In some embodiments, 1 mL, 4 mL, or 10 mL of drug product is administered to the patient intravenously via syringe.

[0743] In some embodiments, the patient is administered acetaminophen prior to being administered the NK cell infusion. In some embodiments, the patient is administered 100, 200, 250, 300, 400, 500, 600, 700, 750, 800, 900, 1000, 1100, 1200, 1250, 1300, 1400, 1500, 1600, 1700, 1750, 1800, 1900, or 2000 mg of acetaminophen. In some embodiments, the acetaminophen is administered to the patient immediately prior to the NK cells. In some embodiments, the acetaminophen is administered 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55. 60, 70, 80, 90, 100, 110, 120, 150, or 180 minutes before the NK cells. In some embodiments, the acetaminophen is administered orally.

[0744] In some embodiments, the patient is administered diphenhydramine prior to being administered the NK cell infusion. In some embodiments, the patient is administered 5, 10, 12.5, 15, 17.5, 20, 25, 30, 40, 50, 60, 70, 75, 80, 90, or 100 mg of diphenhydramine. In some embodiments, the diphenhydramine is administered to the patient immediately prior to the NK cells. In some embodiments, the diphenhydramine is administered 5. 10. 15. 20. 25. 30. 35. 40.

[0745] 45, 50, 55, 60, 70, 80, 90, 100, 110, 120, 150, or 180 minutes before the NK cells. In some embodiments, the acetaminophen is administered orally.

[0746] In some embodiments, the patient is monitored for a period of time before and after administration of NK cells. For example, the patient's vital signs can be monitored. These can include temperature, respiratory rate. heart rate, blood pressure, and oxygen saturation (SaO2) by pulse oximetry. In some cases, at least one vital sign is measured beginning 5, 10, 15 20, 25, or 30 minutes prior to NK cell administration. After NK cell administration, at least one vital sign can be monitored for 1, 2, 3, 4, or 5 hours continuously or at regular or irregular intervals, including approximately every 5. 10, 15, 20, 25, or 30 minutes. In some cases, vital signs can be monitored after NK cell administration until the patient is stable.

Cytokines

[0747] In some embodiments, a cytokine is administered to the patient.

[0748] In some embodiments, the cytokine is administered together with the NK cells as part of a pharmaceutical composition. In some embodiments, the cytokine is administered separately from the NK cells, e.g., as part of a separate pharmaceutical composition.

[0749] In some embodiments, the cytokine is IL-2.

[0750] In some embodiments, the IL-2 is administered subcutaneously.

[0751] In some embodiments, the IL-2 is administered from between 1 to 4 or about 1 to about 4 hours following the conclusion of NK cell administration. In some embodiments, the IL-2 is administered at least 1 hour following the conclusion of NK cell administration. In some embodiments, the IL-2 is administered no more than 4 hours following the conclusion of NK cell administration. In some embodiments, the IL-2 is administered at least 1 hour after and no more than 4 hours following the conclusion of NK cell administration.

[0752] In some embodiments, the IL-2 is administered at up to 10 million IU/M.sup.2, e.g., up to 1 million, 2 million, 3 million, 4 million, 5 million, 6 million, 7 million, 8 million, 9 million, or 10 million IU/m.sup.2.

[0753] In some embodiments, the IL-2 is administered at or at about 1 million, at or at about 2 million, at or at about 3 million, at or at about 4 million, at or at about 5 million, at or at about 6 million, at or at about 7 million, at or at about 8 million, at or at about 9 million, at or at about 10 million IU/M2

[0754] In some embodiments, the IL-2 is administered at or at about 110.sup.6IU/M2. In some embodiments, the IL-2 is administered at or at about 210.sup.6IU/M2.

[0755] In some embodiments, less than 110.sup.6IU/M2 IL-2 is administered to the patient.

[0756] In some embodiments, a flat dose of IL-2 is administered to the patient. In some embodiments, a flat dose of 6 million IU or about 6 million IU is administered to the patient. In some embodiments, IL-2 is not administered to the patient.

Dosing

[0757] An effective amount is an amount sufficient to effect beneficial or desired results. For example, a therapeutic amount is one that achieves the desired therapeutic effect. This amount can be the same or different from a prophylactically effective amount, which is an amount necessary to prevent onset of disease or disease symptoms. An effective amount can be administered in one or more administrations, applications or dosages. A therapeutically effective amount of a therapeutic compound (i.e., an effective dosage) depends on the therapeutic compounds selected. The compositions can be administered one from one or more times per day to one or more times per week: including once every other day. The skilled artisan will appreciate that certain factors may influence the dosage and timing required to effectively treat a subject, including but not limited to the severity of the disease or disorder, previous treatments. the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of the therapeutic compounds described herein can include a single treatment or a series of treatments.

[0758] Dosage, toxicity and therapeutic efficacy of the therapeutic compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50. Compounds which exhibit high therapeutic indices are preferred. While compounds that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.

[0759] The data obtained from cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds may be within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma may be measured, for example, by high performance liquid chromatography.

Combination Therapies

[0760] In some embodiments, the method comprises administering the NK cells described herein, e.g., the CAR-NK cells described herein, in combination with another therapy, e.g., an antibody, an NK cell engager, an antibody drug conjugate (ADC), a chemotherapy drug, e.g., a small molecule drug, an immune checkpoint inhibitor, and combinations thereof. The other therapy can be administered prior to, subsequent to, or simultaneously with administration of the NK cells.

Antibodies

[0761] In some embodiments, the other therapy is an antibody.

[0762] In some embodiments, the antibody binds to a target selected from the group consisting of CD20, HER-2, EGFR, CD38, SLAMF7, GD2, ALK1, AMHR2, CCR2, CD137, CD19, CD26, CD32b, CD33, CD37, CD70, CD73, CD74, CD248, CLDN6, Clever-1, c-MET, CSF-1R, CXCR4, DKK1, DR5, Epha3, FGFR2b, FGFR3, FLT3,, FOLRI, Globo-H, Glypican3, GM1, Grp78, HER-3, HGF, IGF-IR, IL1RAP, IL-8R, ILT4, Integrin alpha V, M-CSF,

[0763] Mesothelin, MIF, MUCI, MUC16, MUCSAC, Myostatin, NKG2A, NOTCH, NOTCH2/3, PIGF, PRL3, PSMA, RORI, SEMA4D, Sialyl Lewis A, Siglec15, TGF-b, TNFR3, TRAIL-R2, VEGF, VEGFR1, VEGFR2, Vimentin, and combinations thereof.

[0764] Suitable antibodies include but are not limited to those shown in Table 8.

TABLE-US-00008 TABLE 8 Antibodies for Combination Therapy Target Drug Name Brand Name Indication(s) Reference CD20 Rituxan Rituximab DLBCL/FL, NHL, Du et al., Auto Immun Highlights CLL, RA, GPA, (2017) 8(1): 12 MPA CD20 Gazyva Obinutuzumab CLL, FL Gagez et al., Curr Opin Oncol. 2014 September; 26(5): 484-91 CD20 Arzerra Ofatumumab CLL Robak, Curr Opin Mol Ther. 2008 June; 10(3): 294-309 CD20 Ocrevus Ocrelizumab RMS, PPMS Genovese et al., Arthritis Rheum. 2008 September; 58(9): 2652-61 CD20 Zevalin Ibritumomab NHL Wiseman et al., Eur J Nucl Med. 2000 July; 27(7): 766-77 CD20 Veltuzumab NHL, CLL Kalaycio et al. Leuk Lymphoma. 2016; 57(4): 803-11 CD20 Bexxar Tositumomab NHL Vose et al., J Clin Oncol. 2000 and Iodine I March; 18(6): 1316-23 131 tositumomab CD20 Ublituximab NHL, CLL, RMS Sawas et al., Br J Haematol. 2017 April; 177(2): 243-253 HER-2 Herceptin Trastuzumab Breast, Gastric Goldenberg, Clin Ther. 1999 February; 21(2): 309-18 HER-2 Perjeta Pertuzumab Breast Agus et al., J Clin Oncol. 2005 Apr. 10; 23(11): 2534-43 HER-2 Margenza Margetuximab Breast Bang et al., Ann Oncol. 2017 Apr. 1; 28(4): 855-861 EGFR Erbitux Cetuximab CRC, HNC Jonker et al., N Engl J Med 2007; 357: 2040-2048 EGFR Vectibix Panitumumab CRC Gibson et al., Clin Colorectal Cancer. 2006 May; 6(1): 29-31 EGFR Portrazza Necitumumab NSCLC Kuenen et al., Clin Cancer Res. 2010 Mar. 15; 16(6): 1915-23 CD38 Darzalex Daratumumab MM de Weers et al., J Immunol. 2011 Feb. 1; 186(3): 1840-8 CD38 Sarclisa Isatuximab MM Martin et al., Blood Cancer J. 2019 Mar. 29; 9(4): 41 SLAMF7 Empliciti Elotuzumab MM Lonial et al., N Engl J Med 2015; 373: 621-631 GD2 Unituxin Dinutuximab NB Hoy, Target Oncol. 2016 Apr; 11(2): 247-53 GD2 Danyelza Naxitamab NB Markham, Drugs. 2021 February; 81(2): 291-296 ALK1 PF- Ascrinvacumab Liver cancer Simonelli et al., Ann Oncol. 2016 03446962 September; 27(9): 1782-7 AMHR2 GM-102 Murlentamab Ovarian Cancer Leary et al., J Clin Oncol. 2019 37: 15_suppl, 2521-2521 CCR2 TAK-202 Plozalizumab Atherosclerosis, Gilbert et al., Am J Cardiol. 2011 Melanoma Mar. 15; 107(6): 906-11 CD137 BMS- Urelumab Melanoma, Segal et al., Clin Cancer Res. 663513 Myeloma, NSCLC 2017 Apr. 15; 23(8): 1929-1936 CD137 PF- Utomilumab Ovarian Cancer Segal et al., Clin Cancer Res. 05082566 2018 Apr. 15; 24(8): 1816-1823 CD19 AMG103 Blinatumomab ALL, NHL Nadafi et al., Int J Mol Cell Med (2015) 4(3): 143-151 CD19 SAR3419 Coltuximab ALL, NHL Nadafi et al. Ravtansine CD19 XmAb 5574 MOR208 ALL, NHL, CLL Nadafi et al. CD19 MEDI-551 MEDI-551 B-cell Nadafi et al. malignancies, CLL, Multiple Myeloma, Scleroderma CD19 SGN-19A Denintuzumab NHL Nadafi et al. Mafodotin CD19 DI-B4 B-cell Nadafi et al. malignancies CD19 Taplitumom Taplitumomab B-cell Nadafi et al. abpaptox paptox malignancies CD19 XmAb 5871 XmAb 5871 Autoimmune Nadafi et al. Diseases CD19 MDX-1342 MDX-1342 CLL, Rheumatoid Nadafi et al. Arthritis CD19 AFM11 AFM11 NHL Nadafi et al. CD19 ADCT-402 Loncastuximab ALL, NHL Yu et al., Journal of Hematology Tesirine & Oncology (2019) 12(94) CD19 Monjuvi Tafasitamab NHL (DLBCL) Hoy, Drugs. 2020 November; 80(16): 1731-1737 CD26 Begedina Begelomab Graft versus host Bacigalupo et al., Bone Marrow disease Transplant. 2020 August; 55(8): 1580-1587 CD32b BI-1206 BI-1206 BCL, CLL Trial ID: NCT04219254 CD33 Mylotarg Gemtuzumab AML Stasi, Expert Opin Biol Ther. Ozogamicin 2008 April; 8(4): 527-40 CD33 SGN-33 Lintuzumab AML Trial ID: NCT02998047 CD37 BI 836826 BI 836826 DLBCL, CLL, Trial ID: NCT02538614 NHL CD37 IMGN529 Naratuximab DLBCL, NHL Yu et al., Journal of Hematology emtansine & Oncology (2019) 12(94) CD37 AGS67E AGS67E DLBCL, NHL Yu et al. CD70 BMS- MDX-1203 DLBCL, MCL Yu et al. 936561 CD70 SGN-75 Vorsetuzumab NHL Yu et al. mafodotin CD73 MEDI9447 Oleclumab Pancreatic cancer Geoghegan et al., Mabs. 2016; 8(3): 454-67 CD73 AK119 AK119 Covid-19, Solid Trial ID: NCT04516564 Tumors CD74 hLL1-DOX Milatuzumab MM Yu et al. doxorubicin CD74 STRO-001 STRO-001 MM, NHL Trial ID: NCT03424603 CD248 Ontecizumab Ontuxizumab MM, Soft tissue D'Angelo et al., Invest New sarcoma Drugs. 2018 February; 36(1): 103-113 CLDN6 IMAB027 ASP1650 Testicular cancer Trial ID: NCT03760081 Clever-1 Clevegen Bexmarilimab Solid tumors Trial ID: NCT03733990 c-MET MetMAb Onartuzumab NSCLC Hughes et al., Trends Cancer (2018) 4(2): 94-97 c-MET AMG-102 Rilotumumab Gastric cancer Waddell et al., Immunotherapy. 2014; 6(12): 1243-53 CSF-1R FPA-008 Cabiralizumab MM, NSCLC Trial ID: NCT04050462 CSF-1R RG-7155 Emactuzumab Ovarian cancer Trial ID: NCT03708224 CSF-1R IMC CS4 LY3022855 MM Trial ID: NCT03153410 CSF-1R AMB 051 AMG 820 Solid tumors Trial ID: NCT04731675 CSF-1R SNDX-6352 Axatilimab Graft versus host Trial ID: NCT04710576 disease CXCR4 BMS- Ulocuplumab Leukemia Bobkov et al., Mol Pharmacol 936564 (2019) 96: 753-764 CXCR4 LY2624587 LY2624587 Metastatic Cancer Bobkov et al. CXCR4 PF- PF-06747143 AML Bobkov et al. 06747143 CXCR4 F50067 hz515H7 MM Bobkov et al. CXCR4 MEDI3185 MEDI3185 Hematologic Bobkov et al. malignancies DKK1 DKN-01 DKN-01 Gastric cancer Wall et al., Expert Opin Investig Drugs. 2020 July; 29(7): 639-644 DKK1 BHQ880 BHQ880 MM Fulciniti et al., Blood. 2009 Jul. 9; 114(2): 371-9 DR5 AD5-10 Zaptuzumab Solid tumors Zhang et al., Theranostics. 2019 Jul. 13; 9(18): 5412-5423 DR5 AMG655 Conatumumab Colon, pancreatic Rosevear et al., Curr Opin cancer Investig Drugs. 2010 June; 11(6): 688-98 DR5 PRO955780 Drozitumab NHL, NSCLC Kang et al., Clin Cancer Res. 2011 May 15; 17(10): 3181-92 DR5 ETR2-ST01 Lexatumumab Solid tumors Plummer et al., Clin Cancer Res. 2007 Oct. 15; 13(20): 6187-94 DR5 CS-1008 Tigatuzumab Solid tumors Reck et al., Lung Cancer. 2013 December; 82(3): 441-8 DR5 DS-8273a Solid tumors Forero et al., Invest New Drugs. 2017 June; 35(3): 298-306 Epha3 KB004 KB004 Glioblastoma Swords et al., Leuk Res. 2016 November; 50: 123-131 FGFR2b FPA-144 Bemarituzumab Gastric cancer Catenacci et al., J Clin Oncol. 2020 Jul. 20; 38(21): 2418-2426 FGFR2b BAY Aprutumab Solid tumors Kim et al., Target Oncol. 2019 1187982 ixadotin October; 14(5): 591-601 FGFR2b BAY- Aprutumab Solid tumors Trial ID: NCT01881217 1179470 FGFR3 LY3076226 LY3076226 Solid tumors Trial ID: NCT02529553 FLT3 IMC-EB10 IMC-EB10 AML Piloto et al., Cancer Res. 2006 May 1; 66(9): 4843-51 AGS 62P1 ASP1235 AML Trial ID: NCT02864290 FOLR1 MORAb- Farletuzumab Ovarian cancer Sato et al., Onco Targets Ther 003 2016 Mar. 7; 9: 1181-8 Globo-H OBI-833 OBI-833 Solid tumors Trial ID: NCT02310464 Globo-H OBI-888 OBI-888 Solid tumors Trial ID: NCT03573544 Globo-H OBI-999 OBI-999 Solid tumors Trial ID: NCT04084366 Glypican3 GC33 Codrituzumab Liver cancer Abou-Alfa et al., J Hepatol. 2016 August; 65(2): 289-95 Glypican3 ERY974 Solid tumors Ishiguro et al., Sci Transl Med. 2017 Oct. 4; 9(410) GM1 BMS986012 BMS-986012 Lung cancer Ponath et al., Clin Cancer Res. 2018 Oct. 15; 24(20): 5178-5189 Grp78 PAT-SM6 PAT-SM6 Multiple myeloma Hensel et al., Melanoma Res. 2013 August; 23(4): 264-75 HER-3 U3-1402 Patritumab NSCLC, Solid Hashimoto et al., Clin Cancer deruxtecan tumors Res. 2019 Dec. 1; 25(23): 7151- 7161 HGF AMG-102 Rilotumumab Solid tumors Waddell et al., Immunotherapy. 2014; 6(12): 1243-53 HGF AV-299 Ficlatuzumab AML, NSCLC Bauman et al., Cancers (Basel). 2020 Jun. 11; 12(6): 1537 HGF L2G7 TAK-701 Solid tumors Okamoto et al., Mol Cancer Ther. 2010 October; 9(10): 2785-92 IGF-1R IMC-A12 Cixutumumab EWS, HCC Chen et al., Chin J Cancer (2013) 32(5): 242-252 IGF-1R CP-751 Figitumumab EWS, ACC Chen et al. IGF-1R MK-0646 Dalotuzumab Colorectal cancer Chen et al. IGF-1R AMG 479 Ganitumab EWS, DRCT Chen et al. IGF-1R R1507 EWS Chen et al. IGF-1R AVE-1642 VRDN 001 MM, Breast Trial ID: NCT01233895 cancer IL1RAP CAN04 Nidanilimab NSCLC Awada et al., J Clin Oncol. 2019 May; 37: 2504-2504 IL-8R BMS- HuMax-IL8 Covid-19, NSCLC Bilusic et al., J Immunother 986253 Cancer. 2019 Sep. 5; 7(1): 240 ILT4 JTX-8064 JTX-8064 Solid tumors Trial ID: NCT04669899 Integrin IMGN388 IMGN388 Solid tumors Trial ID: NCT00721669 alpha V Integrin CNTO-95 Intetumumab MM O'Day et al., Br J Cancer. 2011 alpha V Jul. 26; 105(3): 346-52 Integrin EMD525797 Abituzumab Colorectal cancer Jiang et al., Mol Cancer Res. alpha V 2017 July; 15(7): 875-883 Integrin MEDI-522 Etaracizumab MM, Colorectal Hersey et al., Cancer. 2010 Mar. alpha V cancer 15; 116(6): 1526-34 Integrin VPI-2690B VPI-2690B Diabetic Trial ID: NCT02251067 alpha V nephropathies M-CSF MCS-110 Lacnotuzumab Breast cancer, Pognan et al., J Pharmacol Exp Gastric cancer Ther. 2019 June; 369(3): 428-442 Mesothelin MORAb- amatuximab Mesothelioma Baldo et al., Onco Targets Ther. 009 2017 Nov. 8; 10: 5337-5353 Mesothelin SS1(dsFv)- SS1P Neoplasms Hassan et al., J Clin Oncol. 2016 PE38 December; 34(34): 4171-4179 Mesothelin BAY 94- Anetumab Mesothelioma Hassan et al., J Clin Oncol. 2020 9343 ravtansine Jun. 1; 38(16): 1824-1835 Mesothelin RG7600 DMOT4039A Pancreatic cancer, Hassan et al., J Clin Oncol. 2016 ovarian cancer December; 34(34): 4171-4179 Mesothelin BMS- BMS-986148 Solid Tumors Hassan et al., J Clin Oncol. 2016 986148 December; 34(34): 4171-4179 MIF BAX69 Imalumab Colorectal cancer Mahalingham et al., Br J Clin Pharmacol. 2020 September; 86(9): 1836-1848 MUC1 huC242- Cantuzumab Pancreatic cancer Tolcher et al., J Clin Oncol. 2003 DM1 mertansine Jan. 15; 21(2): 211-22 MUC1 hPAM4 Clivatuzumab Pancreatic cancer Liu et al., Oncotarget. 2015 Feb. 28; 6(6): 4274-85 MUC1 GT-MAB Gatipotuzumab Ovarian cancer Heublin et al., Int J Mol Sci. 2019 2.5-GEX Jan. 12; 20(2): 295 MUC1 mAb- AR20.5 Pancreatic cancer de Bono et al., Ann Oncol. 2004 AR20.5 December; 15(12): 1825-33 MUC16 ACA 125 Abagovomab Ovarian cancer Sabbatini et al., J Clin Oncol. 2013 Apr. 20; 31(12): 1554-61 MUC16 DMUC5754A Sofituzumab Ovarian cancer Liu et al., Ann Oncol. 2016 vedotin November; 27(11): 2124-2130 MUC16 DMUC4064A THIOMAB Ovarian cancer Trial ID: NCT02146313 MUC5AC PAM4 Clivatuzumab PDAC Gold et al., Molecular Cancer (2013) 12: 143 MUC5AC NPC-1C Ensituximab Pancreatic cancer Kim et al., Clin Cancer Res. 2020 Jul. 15; 26(14): 3557-3564 Myostatin MYO-029 Stamulumab Muscular atrophy, Trial ID: NCT00563810 Muscular dystrophies Myostatin PF- Domagrozumab Duchenne Wagner et al., Neuromuscul 06252616 muscular Disord. 2020 June; 30(6): 492-502 dystrophy Myostatin LY- Landogrozumab Muscular atrophy, Golan et al., J Cachexia 2495655 Pancreatic cancer Sarcopenia Muscle. 2018 October; 9(5): 871-879 Myostatin REGN-1033 Trevogrumab Muscular atrophy Trial ID: NCT01720576 Myostatin SRK-015 Apitegromab Spinal muscular Trial ID: NCT03921528 atrophy NKG2A IPH2201 Monalizumab Breast cancer; Andre et al., Cell. 2018 Dec. NSCLC 13: 175(7): 1731-1743 NOTCH OMP- Demcizumab NSCLC Takebe et al., Pharmacol Ther 21M18 (2014) 141(2): 140-149 NOTCH REGN421/ Enoticumab NSCLC, Ovarian Takebe et al. SAR153192 cancer NOTCH OPM- Brontictuzumab Solid tumors Takebe et al. 52M51 NOTCH2/3 OMP-59R5 Tarextumab Sarcomas, Rectal Takebe et al. cancer PIGF RO5323441 TB-403 Solid tumors Martinsson-Niskanen et al., Clin Ther. 2011 September; 33(9): 1142-9 PRL3 PRL3- PRL3-zumab Solid tumors Trial ID: NCT04452955 ZUMAB PSMA Capromab Capromab Prostate cancer Trial ID: NCT00992745 pendetide PSMA MT112 Pasotuxizumab Prostate cancer Hummel et al., Immunotherapy. 2021 February; 13(2): 125-141 PSMA MDX1201- Prostate cancer Trial ID: NCT02048150 A488 PSMA APVO 414 MOR209/ES414 Prostate cancer Hernandez-Hoyos et al., Mol Cancer Ther. 2016 September; 15(9): 2155-65 PSMA ARX-517 ARX517 Prostate cancer Trial ID: NCT04662580 PSMA ADCT 401 MEDI3726 Prostate cancer Cho et al., Mol Cancer Ther. 2018 October.; 17(10): 2176-2186 PSMA JNJ-63898081 Prostate cancer Trial ID: NCT03926013 PSMA PSMA TTC BAY 2315497 Prostate cancer Hammer et al., Clin Cancer Res. 2020 Apr. 15; 26(8): 1985-1996 PSMA TLX592 Prostate cancer Trial ID: NCT04726033 PSMA DOTA- Rosopatamab Prostate cancer Vallabhajosula et al., Curr HUJ-591 tetraxetan Radiopharm. 2016; 9(1): 44-53 PSMA PSMA ADC Prostate cancer Petrylak et al., Prostate. 2020 January; 80(1): 99-108 ROR1 UC-961 Cirmtuzumab CLL, MCL Choi et al., Cell Stem Cell. 2018 Jun. 1; 22(6): 951-959 SEMA4D VX15/2503 Pepinemab NSCLC, MM Sialyl MVT-5873 MVT-5873 Colorectal cancer Gupta et al., J Gastrointest Oncol. Lewis A 2020 April; 11(2): 231-235 Sialyl AbGn-7 AbGn-7 Gastric cancer Trial ID: NCT01466569 Lewis A Siglec15 NC318 NC318 Solid tumors Trial ID: NCT03665285 TGF-b SRK-181 Solid tumors Trial ID: NCT04291079 TGF-b M-7824 Bintrafusp alfa NSCLC, Solid Yoo et al., J Immunother Cancer. tumors 2020 May; 8(1): e000564 TGF-b GC-1008 Fresolimumab MM Rice et al., J Clin Invest. 2015 Jul. 1; 125(7): 2795-807 TGF-b LY2382770 Diabetic Trial ID: NCT01113801 nephropathies TGF-b NIS-793 NIS793 Pancreatic cancer Trial ID: NCT04390763: TGF-b SAR439459 Solid tumors Trial ID: NCT03192345 TGF-b Metelimumab Cancer, Lord et al., Mabs. 2018 Scleroderma April; 10(3): 444-452 TGF-b IMC TR1 LY3022859 Solid tumors Tolcher et al., Cancer Chemother Pharmacol. 2017 April; 79(4): 673- 680 TNFR3 Baminercept BG9924 Rheumatoid Trial ID: NCT00664716 arthritis TRAIL-R2 CS-1008 Tigatuzumab Breast cancer, Cheng et al., J Hepatol. 2015 NSCLC October; 63(4): 896-904 TRAIL-R2 AMG-655 Conatumumab Solid tumors Bajaj et al., Expert Opin Biol Ther. 2011 November; 11(11): 1519-24 TRAIL-R2 PRO-95780 Drozitumab NHL, NSCLC Lima et al., Cancer Invest. 2012 December; 30(10): 727-31 TRAIL-R2 HGS-ETR2 Lexatumumab Solid tumors Plummer et al., Clin Cancer Res. 2007 Oct. 15; 13(20): 6187-94 TRAIL-R2 TAS-266 TAS266 Solid tumors Trial ID: NCT01529307 TRAIL-R2 GEN1029 Benufutamab Solid tumors Overdijk et al., Mol Cancer Ther. 2020 October; 19(10): 2126-2138 TRAIL-R2 RO- RG7386 Solid tumors Brunker et al., Mol Cancer Ther. 6874813 2016 May; 15(5): 946-57 TRAIL-R2 JCT-205 INBRX-109 Solid tumors Trial ID: NCT03715933 VEGF Avastin Bevacizumab NSCLC, MM Garcia et al., Cancer Treat Rev. 2020 June; 86: 102017 VEGF Lucentis Ranibizumab Macular Gross et al., JAMA Ophthalmol. de generation 2018 Oct. 1; 136(10): 1138-1148 VEGFR1 IMC-18F1 Icrucumab Breast cancer LoRusso et al., Invest New Drugs. 2014 April; 32(2): 303-11 VEGFR2 Cyramza Ramucirumab NSCLC, Khan et al., Expert Opin Biol Colorectal cancer Ther. 2019 November; 19(11): 1135-1141 VEGFR2 Tanibirumab Olinvacimab Glioblastoma Lee et al., Drug Des Devel Ther. 2018 Mar. 8; 12: 495-504 VEGFR2 Gentuximab Solid tumors Chamie et al., JAMA Oncol. 2017 Jul. 1; 3(7): 913-920 VEGFR2 CDP-791 Alacizumab NSCLC Trial ID: NCT00152477 pegol VEGFR2 HLX-06 Vulinacimab Solid tumors Trial ID: NCT03494231 VEGFR2 MSB0254 Solid tumors Trial ID: NCT04381325 VEGFR2 AK109 Solid tumors Trial ID: NCT04547205 Vimentin CLNH11 Pritumumab Glioma Babic et al., Hum Antibodies. 2018 Feb. 5; 26(2): 95-101 Vimentin 86C Glioblastoma Stouhalova et al., Cancers (2020) 12(1): 184

Small Molecule/Chemotherapy Drugs

[0765] In some embodiments, the additional therapy is a small molecule drug. In some embodiments, the additional therapy is a chemotherapy drug. In some embodiments, the additional therapy is a small molecule chemotherapy drug. Such small molecule drugs can include existing standard-of-care treatment regimens to which adoptive NK cell therapy is added. In some cases, the use of the NK cells described herein can enhance the effects of small molecule drugs, including by enhancing the efficacy, reducing the amount of small molecule drug necessary to achieve a desired effect, or reducing the toxicity of the small molecule drug.

[0766] In some embodiments, the drug is selected from the group consisting of

[0767] In some embodiments, the drug is [(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4-acetyloxy-1,9,12-trihydroxy-15-[(2R,3S)-2-hydroxy-3-[(2-methylpropan-2-yl)oxycarbonylamino]-3-phenylpropanoyl] oxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04,7] heptadec-13-en-2-yl]benzoate (docetaxel) or a pharmaceutically acceptable salt thereof.

[0768] In some embodiments, the drug is [(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4,12-diacetyloxy-15-[(2R,3S)-3-benzamido-2-hydroxy-3-phenylpropanoyl] oxy-1,9-dihydroxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04.7] heptadec-13-en-2-yl]benzoate (paclitaxel) or a pharmaceutically acceptable salt thereof.

[0769] In some embodiments, the drug is 6-N-(4,4-dimethyl-5H-1,3-oxazol-2-yl)-4-N-[3-methyl-4-([1.2.4]triazolo[1,5-a]pyridin-7-yloxy)phenyl] quinazoline-4.6-diamine (tucatinib) or a pharmaceutically acceptable salt thereof.

[0770] In some embodiments, the drug is pentyl N-[1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-methyloxolan-2-yl]-5-fluoro-2-oxopyrimidin-4-yl]carbamate (capecitabine) or a pharmaceutically acceptable salt thereof.

[0771] In some embodiments, the drug is azanide: cyclobutane-1,1-dicarboxylic acid: platinum (2+) (carboplatin) or a pharmaceutically acceptable salt thereof.

[0772] In some embodiments, the drug is methyl (1R,9R, 10S,11R,12R, 19R)-11-acetyloxy-12-ethyl-4-[(12S,14R)-16-ethyl-12-methoxycarbonyl-1,10-diazatetracyclo[12.3.1.03.11.04.9] octadeca-3 (11) , 4,6,8,15-pentaen-12-yl]-10-hydroxy-5-methoxy-8-methyl-8,16-diazapentacyclo[10.6.1.01.9.02.7.016.19] nonadeca-2,4,6, 13-tetraene-10-carboxylate (vinorelbine) or a pharmaceutically acceptable salt thereof.

[0773] In some embodiments, the drug is N-[3-chloro-4-[(3-fluorophenyl) methoxy]phenyl]-6-[5-[(2-methylsulfonylethylamino)methyl] furan-2-vl] quinazolin-4-amine (lapatinib) or a pharmaceutically acceptable salt thereof.

[0774] In some embodiments, the drug is (E)-N-[4-[3-chloro-4-(pyridin-2-ylmethoxy) anilino]-3-cyano-7-ethoxyquinolin-6-yl]-4-(dimethylamino) but-2-enamide (neratinib) or a pharmaceutically acceptable salt thereof.

[0775] In some embodiments, the drug is 6-acetyl-8-cyclopentyl-5-methyl-2-[(5-piperazin-1-ylpyridin-2-yl)amino]pyrido[2,3-d]pyrimidin-7-one (palbociclib) or a pharmaceutically acceptable salt thereof.

[0776] In some embodiments, the drug is 7-cyclopentyl-N,N-dimethyl-2-[(5-piperazin-1-ylpyridin-2-yl)amino]pyrrolo[2,3-d]pyrimidine-6-carboxamide (ribociclib) or a pharmaceutically acceptable salt thereof.

[0777] In some embodiments, the drug is N-[5-[(4-ethylpiperazin-1-yl)methyl] pyridin-2-yl]-5-fluoro-4-(7-fluoro-2-methyl-3-propan-2-ylbenzimidazol-5-yl)pyrimidin-2-amine (abemaciclib) or a pharmaceutically acceptable salt thereof.

[0778] In some embodiments, the drug is (1R,9S,12S,15R,16E, 18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1S,3R,4R)-4-(2-hydroxyethoxy)-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04.9] hexatriaconta-16,24,26,28-tetraene-2.3.10.14.20-pentone (everolimus) or a pharmaceutically acceptable salt thereof.

[0779] In some embodiments, the drug is (2S)-1-N-[4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl) pyridin-4-yl]-1,3-thiazol-2-yl]pyrrolidine-1,2-dicarboxamide (alpelisib) or a pharmaceutically acceptable salt thereof.

[0780] In some embodiments, the drug is 4-[[3-[4-(cyclopropanecarbonyl) piperazine-1-carbonyl]-4-fluorophenyl]methyl]-2H-phthalazin-1-one (olaparib) or a pharmaceutically acceptable salt thereof.

[0781] In some embodiments, the drug is (11S, 12R)-7-fluoro-11-(4-fluorophenyl)-12-(2-methyl-1,2,4-triazol-3-yl)-2,3,10-triazatricyclo[7.3.1.05.13] trideca-1,5 (13).6,8-tetraen-4-one (talazoparib) or a pharmaceutically acceptable salt thereof.

[0782] In some embodiments, the drug is N-[2-[2-(dimethylamino)ethyl-methylamino]-4-methoxy-5-[[4-(1-methylindol-3-yl)pyrimidin-2-yl]amino]phenyl]prop-2-enamid (osimertinib) or a pharmaceutically acceptable salt thereof.

[0783] In some embodiments, the drug is N-(3-chloro-4-fluorophenyl)-7-methoxy-6-(3-morpholin-4-ylpropoxy) quinazolin-4-amine (gefitinib) or a pharmaceutically acceptable salt thereof.

[0784] In some embodiments, the drug is N-(3-ethynylphenyl)-6.7-bis(2-methoxyethoxy) quinazolin-4-amine (erlotinib) or a pharmaceutically acceptable salt thereof.

[0785] In some embodiments, the drug is (E)-N-[4-(3-chloro-4-fluoroanilino)-7-[(3S)-oxolan-3-ylJoxyquinazolin-6-yl]-4-(dimethylamino) but-2-enamide (afatinib) or a pharmaceutically acceptable salt thereof.

[0786] In some embodiments, the drug is azane: dichloroplatinum (cisplatin, platinol) or a pharmaceutically acceptable salt thereof.

[0787] In some embodiments, the drug is azanide: cyclobutane-1,1-dicarboxylic acid: platinum (2+) (carboplatin) or a pharmaceutically acceptable salt thereof

[0788] In some embodiments, the drug is 4-amino-1-[(2R,4R,5R)-3,3-difluoro-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl|pyrimidin-2-one (gemcitabine) or a pharmaceutically acceptable salt thereof.

[0789] In some embodiments, the drug is (2S)-2-[[4-[2-(2-amino-4-oxo-3,7-dihydropyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]amino]pentanedioic acid (pemetrexed) or a pharmaceutically acceptable salt thereof.

[0790] In some embodiments, the drug is N,N-bis(2-chloroethyl)-2-oxo-1,3,25-oxazaphosphinan-2-amine (cyclophosphamide) or a pharmaceutically acceptable salt thereof.

[0791] In some embodiments, the drug is (2R,3S,4S,5R)-2-(6-amino-2-fluoropurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol (fludarabine) or a pharmaceutically acceptable salt thereof.

[0792] In some embodiments, the drug is (7S,9S)-7-[(2R,4S.5S,6S)-4-amino-5-hydroxy-6-methyloxan-2-yl|oxy-6,9, 11-trihydroxy-9-(2-hydroxyacetyl)-4-methoxy-8,10-dihydro-7H-tetracene-5,12-dione (doxorubicin) or a pharmaceutically acceptable salt thereof.

[0793] In some embodiments, the drug is methyl (1R,9R, 10S, 11R, 12R, 19R)-11-acetyloxy-12-ethyl-4-[(13S,15S, 17S)-17-ethyl-17-hydroxy-13-methoxycarbonyl-1, 11-diazatetracyclo[13.3.1.04.12.05.10] nonadeca-4 (12),5,7,9-tetraen-13-yl]-8-formyl-10-hydroxy-5-methoxy-8, 16-diazapentacyclo[10.6.1.01.9.02.7.016.19] nonadeca-2,4,6, 13-tetraene-10-carboxylate (vincristine) or a pharmaceutically acceptable salt thereof.

[0794] In some embodiments, the drug is (8S,9S, 10R,13S,14S, 17R)-17-hydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-6,7,8,9, 12, 14, 15, 16-octahydrocyclopenta[a]phenanthrene-3,11-dione (prednisone) or a pharmaceutically acceptable salt thereof.

[0795] In some embodiments, the drug is N,3-bis(2-chloroethyl)-2-oxo-1,3,225-oxazaphosphinan-2-amine (ifosfamide) or a pharmaceutically acceptable salt thereof.

[0796] In some embodiments, the drug is (5S,5aR,8aR,9R)-5-[[(2R,4aR,6R,7R,8R,8aS)-7,8-dihydroxy-2-methyl-4,4a,6, 7,8,8a-hexahydropyrano[3,2-d][1,3]dioxin-6-yl]oxy]-9-(4-hydroxy-3,5-dimethoxyphenyl)-5a,6,8a,9-tetrahydro-5H-[2] benzofuro [6,5-f][1,3]benzodioxol-8-one (etopside) or a pharmaceutically acceptable salt thereof.

[0797] In some embodiments, the drug is (8S,9R,10S,11S,13S,14S,16R,17R)-9-fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8, 11, 12, 14,15, 16-octahydrocyclopenta[a]phenanthren-3-one (dexamethasone) or a pharmaceutically acceptable salt thereof.

[0798] In some embodiments, the drug is (8S,9R, 10S,11S,13S,14S,16R,17R)-9-fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-6,7,8, 11, 12, 14, 15,16-octahydrocyclopenta [alphenanthren-3-one (cytarabine) or a pharmaceutically acceptable salt thereof.

[0799] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with an antibody, e.g., a monoclonal antibody, an antibody-drug conjugate (ADC), a kinase inhibitor, a CDK4/5 inhibitor, an mTOR inhibitor, a PI3K inhibitor, a PARP inhibitor, or a combination thereof.

[0800] In some embodiments, the antibody is selected from the group consisting of trastuzumab, pertuzumab, margetuximab, and combinations thereof.

[0801] In some embodiments, the antibody-drug conjugate is selected from the group consisting of ado-trastuzumab emtansine, fam-trastuzumab deruxtecan, sacituzumab govitecan, and combinations thereof.

[0802] In some embodiments, the kinase inhibitor is selected from the group consisting of lapatinib, neratinib, tucatinib, and combinations thereof.

[0803] In some embodiments, the CDK4/6 inhibitor is selected from the group consisting of palbociclib, ribociclib, abemaciclib, and combinations thereof.

[0804] In some embodiments, the mTOR inhibitor is everolimus.

[0805] In some embodiments, the PI3K inhibitor is alpelisib.

[0806] In some embodiments, the PARP inhibitor is selected from the group consisting of olaparib, talazoparib, and combinations thereof.

[0807] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with pertuzumab (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof) and docetaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the pertuzumab (or FDA-approved biosimilar thereof) is administered at 840 mg IV day I followed by 420 mg IV. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 7 mg/kg IV day 1 followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the docetaxel (or pharmaceutically acceptable salt thereof) is administered at 75-100 mg/m.sup.2 IV day 1 cycled every 21 days.

[0808] In some embodiments, the NK cells, e.g., the CAR-NK cells, e.g., AB-201 cells, are administered in combination with pertuzumab (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof), and paclitaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the pertuzumab (or FDA-approved biosimilar thereof) is administered at 840 mg IV day I followed by 420 mg IV, cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the paclitaxel (or pharmaceutically acceptable salt thereof) is administered at 80 mg/m.sup.2 IV day 1 weekly or 175 mg/m.sup.2 day I cycled every 21 days.

[0809] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with tucatinib (or pharmaceutically acceptable salt thereof), trastuzumab (or FDA-approved biosimilar thereof), and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the tucatinib (or FDA-approved biosimilar thereof) is administered at 300 mg orally twice daily on days 1-21. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered at 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration. In some embodiments, the capecitabine (or FDA-approved biosimilar thereof) is administered at 1000 mg/m.sup.2 orally twice daily on days 1-14. In some embodiments, the administration of tucatinib (or FDA-approved biosimilar thereof), trastuzumab (or FDA-approved biosimilar thereof), and capecitabine (or pharmaceutically acceptable salt thereof) is cycled every 21 days.

[0810] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with ado-trastuzumab emtansine (T-DM1) (or FDA-approved biosimilar thereof). In some embodiments, the ado-trastuzumab emtansine (T-DM1) (or FDA-approved biosimilar thereof) is administered at 3.6 mg/kg IV day 1, cycled every 21 days.

[0811] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with fam-trastuzumab deruxtecan-nxki (or FDA-approved biosimilar thereof). In some embodiments, the fam-trastuzumab deruxtecan-nxki (or FDA-approved biosimilar thereof) is administered at 5.4 mg/kg IV day 1, cycled every 21 days. In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g.,

[0812] AB-201 cells, are administered in combination with paclitaxel/carboplatin (or pharmaceutically acceptable salts thereof) and trastuzumab (or FDA-approved biosimilar thereof). In some embodiments, the carboplatin/paclitaxel (or pharmaceutically acceptable salts thereof) is administered at AUC 6 IV day 1 carboplatin and 175 mg/m.sup.2 IV day 1 paclitaxel), cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0813] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with paclitaxel/carboplatin (or pharmaceutically acceptable salts thereof) and trastuzumab (or FDA-approved biosimilar thereof). In some embodiments, the carboplatin/paclitaxel (or pharmaceutically acceptable salts thereof) is administered at AUC 2 IV carboplatin and 80 mg/m.sup.2 IV day 1 paclitaxel), days 1, 8, and 15, cycled every 28 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day 1 followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0814] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with trastuzumab (or FDA-approved biosimilar thereof) and paclitaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the paclitaxel (or pharmaceutically acceptable salt thereof) is administered at 175 mg/m.sup.2 IV day 1 cycled every 21 days or 80-90 mg/m.sup.2 IV day 1 weekly. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0815] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with trastuzumab (or FDA-approved biosimilar thereof) and docetaxel (or pharmaceutically acceptable salt thereof). In some embodiments, the docetaxel (or pharmaceutically acceptable salt thereof) is administered at 80-100 mg/m.sup.2 IV day 1 cycled every 21 days or 35 mg/m.sup.2 IV days 1, 8, and 15 weekly. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day 1 followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0816] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with trastuzumab (or FDA-approved biosimilar thereof) and vinorelbine (or pharmaceutically acceptable salt thereof). In some embodiments, the vinorelbine (or pharmaceutically acceptable salt thereof) is administered at 25 mg/m.sup.2 IV day 1 weekly or 20-35 mg/m.sup.2 IV days 1 and 8, cycled every 21 days, or 25-30 mg/m.sup.2 IV days 1. 8, and 15, cycled every 28 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day 1 followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0817] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with trastuzumab (or FDA-approved biosimilar thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the capecitabine (or pharmaceutically acceptable salt thereof) is administered at 1000-1250 mg/m.sup.2 PO twice daily days 1-14 cycled every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day 1 followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0818] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with lapatinib (or pharmaceutically acceptable salt thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the lapatinib (or pharmaceutically acceptable salt thereof) is administered at 1250 mg/m.sup.2 PO daily days 1-21. In some embodiments, the capecitabine (or pharmaceutically acceptable salt thereof) is administered at 1000 mg/m.sup.2 PO twice daily days 1-14, cycled every 21 days.

[0819] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with trastuzumab (or FDA-approved biosimilar thereof) and lapatinib (or pharmaceutically acceptable salt thereof). In some embodiments, the administered (or pharmaceutically acceptable salt thereof) is administered at 1000 mg/m.sup.2 PO daily. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered 4 mg/kg IV day I followed by 2 mg/kg IV weekly or 8 mg/kg IV day I followed by 6 mg/kg IV day I every 21 days. In some embodiments, the trastuzumab (or FDA-approved biosimilar thereof) is administered as a trastuzumab (or FDA-approved biosimilar thereof) and hyaluronidase-oysk injection for subcutaneous administration.

[0820] In some embodiments, the NK cells, e.g., the CAR-NK cells described herein, e.g., AB-201 cells, are administered in combination with neratinib (or pharmaceutically acceptable salt thereof) and capecitabine (or pharmaceutically acceptable salt thereof). In some embodiments, the neratinib is administered at 240 mg/m.sup.2 PO daily on days 1-21. Ins ome embodiments, the capecitabine is administered at 750 mg/m.sup.2 PO twice daily on days 1-14, cycled every 21 days.

NK Cell Engagers

[0821] In some embodiments, the additional therapy is an NK cell engager, e.g., a bispecific or trispecific antibody.

[0822] In some embodiments, the NK cell engager is a bispecific antibody against CD16 and a disease-associated antigen, e.g., cancer-associated antigen, e.g., an antigen of cancers described herein, e.g, HER2. In some embodiments, the NK cell engager is a trispecific antibody against CD16 and two disease-associated antigens, e.g., cancer-associated antigens, e.g., antigens of cancers described herein.

Checkpoint Inhibitors

[0823] In some embodiments, the additional therapy is an immune checkpoint inhibitor.

[0824] In some embodiments, the immune checkpoint inhibitor is selected from the group consisting of a PD-1 inhibitor, a PD-L1 inhibitor, a CTLA-4 inhibitor, and combinations thereof.

[0825] In some embodiments, the immune checkpoint inhibitor is selected from the group consisting of a PD-1 inhibitor, a PD-L1 inhibitor, a CTLA-4 inhibitor, a VISTA inhibitor, a BTLA inhibitor, a TIM-3 inhibitor, a KIR inhibitor, a LAG-3 inhibitor, a TIGIT inhibitor, a CD-96 inhibitor, a SIRPa inhibitor, and combinations thereof.

[0826] In some embodiments, the immune checkpoint inhibitor is selected from the group consisting of a PD-1 inhibitor, a PD-L1 inhibitor, a CTLA-4 inhibitor, a LAG-3 (CD223) inhibitor, a TIM-3 inhibitor, a B7-H3 inhibitor, a B7-H4 inhibitor, an A2aR inhibitor, a CD73 inhibitor, a NKG2A inhibitor, a PVRIG/PVRL2 inhibitor, a CEACAMI inhibitor, a CEACAM 5 inhibitor, a CEACAM 6 inhibitor, a FAK inhibitor, a CCL2 inhibitor, a CCR2 inhibitor, a LIF inhibitor, a CD47 inhibitor, a SIRPa inhibitor, a CSF-1 inhibitor, an M-CSF inhibitor, a CSF-1R inhibitor, an IL-1 inhibitor, an IL-1R3 inhibitor, an IL-RAP inhibitor, an IL-8 inhibitor, a SEMA4D inhibitor, an Ang-2 inhibitor, a CELVER-1 inhibitor, an Axl inhibitor, a phsphatidylserine inhibitor, and combinations thereof.

[0827] In some embodiments, the immune checkpoint inhibitor is selected from those shown in Table 9, or combinations thereof.

TABLE-US-00009 TABLE 9 Exemplary Immune Checkpoint Inhibitors Target Inhibitor LAG-3 (CD223) LAG525 (IMP701), REGN3767 (R3767), BI 754.091, tebotelimab (MGD013), eftilagimod alpha (IMP321), FS118 TIM-3 MBG453, Sym023, TSR-022 B7-H3, B7-H4 MGC018, FPA150 A2aR EOS100850, AB928 CD73 CPI-006 NKG2A Monalizumab PVRIG/PVRL2 COM701 CEACAM1 CM24 CEACAM 5/6 NEO-201 FAK Defactinib CCL2/CCR2 PF-04136309 LIF MSC-1 CD47/SIRP Hu5F9-G4 (5F9), ALX148, TTI-662, RRx-001 CSF-1 Lacnotuzumab (MCS110), LY3022855, SNDX-6352, (M-CSF)/CSF-1R emactuzumab (RG7155), pexidartinib (PLX3397) IL-1 and IL-IR3 CAN04, Canakinumab (ACZ885) (IL-1RAP) IL-8 BMS-986253 SEMA4D Pepinemab (VX15/2503) Ang-2 Trebananib CLEVER-1 FP-1305 Axl Enapotamab vedotin (EnaV) Phospha- Bavituximab tidylserine

[0828] In some embodiments, the immune checkpoint inhibitor is an antibody.

[0829] In some embodiments, the PD-1 inhibitor is selected from the group consisting of pembrolizumab, nivolumab, toripalimab, cemiplimab-rwlc, sintilimab, and combinations thereof.

[0830] In some embodiments, the PD-L1 inhibitor is selected from the group consisting of atezolizumab, durvalumab, avolumab, and combinations thereof.

[0831] In some embodiments, the CTLA-4 inhibitor is ipilimumab.

[0832] In some embodiments, the PD-1 inhibitor is selected from the group of inhibitors shown in Table 10.

TABLE-US-00010 TABLE 10 Exemplary PD-1 Inhibitor Antibodies Name Internal Name Antigen Company nivolumab Opdivo, ONO-4538, MDX- PD-1 BMS, Medarex, Ono 1106, BMS-936558, 5C4 pembrolizumab Keytruda, MK-3475, SCH PD-1 Merck (MSD), Schering- 900475, lambrolizumab Plough toripalimab JS001, JS-001, TAB001, PD-1 Junmeng Biosciences, Shanghai Triprizumab Junshi, TopAlliance Bio cemiplimab- Libtayo, cemiplimab, PD-1 Regeneron, Sanofi rwlc REGN2810 sintilimab Tyvyt, IBI308 PD-1 Adimab, Innovent, Lilly MEDI0680 AMP-514 PD-1 Amplimmune, Medimmune LZM009 PD-1 Livzon vudalimab XmAb20717 CTLA4, PD-1 Xencor SI-B003 CTLA4, PD-1 Sichuan Baili Pharma, Systimmune Sym021 Symphogen patent anti-PD-1 PD-1 Symphogen LVGN3616 PD-1 Lyvgen Biopharma MGD019 CTLA4, PD-1 MacroGenics MEDI5752 CTLA4, PD-1 Medimmune CS1003 PD-1 Cstone Pharma IBI319 IBI-319 PD-1, Innovent, Lilly Undisclosed IBI315 IBI-315 HER2/neu, PD-1 Beijing Hanmi, Innovent budigalimab ABBV-181, PR-1648817 PD-1 Abbvie Sunshine 609A PD-1 Sunshine Guojian Pharma Guojian patent anti-PD-1 F520 PD-1 Shandong New Time Pharma RO7247669 LAG-3, PD-1 Roche izuralimab XmAb23104 ICOS, PD-1 Xencor LY3434172 PD-1, PD-L1 Lilly, Zymeworks SG001 PD-1 CSPC Pharma QL1706 PSB205 CTLA4, PD-1 Sound Biologics AMG 404 AMG404 PD-1 Amgen MW11 PD-1 Mabwcll GNR-051 PD-1 IBC Generium Ningbo Cancer HerinCAR-PD1 PD-1 Ningbo Cancer Hosp. Hosp. anti-PD- 1 CAR Chinese PLA PD-1 Chinese PLA Gen. Hosp. Gen. Hosp. anti-PD-1 cetrelimab JNJ-63723283 PD-1 Janssen Biotech TY101 PD-1 Tayu Huaxia AK112 PD-1, VEGF Akeso EMB-02 LAG-3, PD-1 EpimAb pidilizumab CT-011, hBat-1, MDV9300 PD-1 CureTech, Medivation, Teva sasanlimab PF-06801591, RN-888 PD-1 Pfizer balstilimab AGEN2034, AGEN-2034 PD-1 Agenus, Ludwig Inst., Sloan- Kettering geptanolimab CBT-501, GB226, GB 226, PD-1 CBT Pharma, Genor Genolimzumab, Genormab RO7121661 PD-1, TIM-3 Roche AK104 CTLA4, PD-1 Akeso pimivalimab JTX-4014 PD-1 Jounce IBI318 IBI-318 PD-1, PD-L1 Innovent, Lilly BAT1306 PD-1 Bio-Thera Solutions ezabenlimab BI754091, BI 754091 PD-1 Boehringer Henan Cancer Teripalimab PD-1 Henan Cancer Hospital Hospital anti- PD-1 tebotelimab LAG-3, PD-1 MacroGenics sindelizumab PD-1 Nanjing Medical U. dostarlimab ANB011, TSR-042, ABT1 PD-1 AnaptysBio, Tesaro tislelizumab BGB-A317 PD-1 BeiGene, Celgene spartalizumab PDR001, BAP049 PD-1 Dana-Farber, Novartis retifanlimab MGA012, INCMGA00012 PD-1 Incyte, MacroGenics camrelizumab SHR-1210 PD-1 Incyte, Jiangsu Hengrui, Shanghai Hengrui zimberelimab WBP3055, GLS-010, AB122 PD-1 Arcus, Guangzhou Gloria Bio, Harbin Gloria Pharma, WuXi Biologics penpulimab AK105 PD-1 Akeso, HanX Bio, Taizhou Hanzhong Bio prolgolimab BCD-100 PD-1 Biocad HX008 PD-1 Taizhou Hanzhong Bio, Taizhou HoudeAoke Bio SCT-I10A PD-1 Sinocelltech serplulimab HLX10 PD-1 Henlix

[0833] In some embodiments, the PD-L1 inhibitor is selected from the group of inhibitors shown in Table 11.

TABLE-US-00011 TABLE 11 Exemplary PD-L1 Inhibitor Antibodies Name Internal Name Antigen Company durvalumab Imfinzi, MEDI-4736, PD-L1 AstraZeneca, Celgene, Medimmune MEDI4736 atezolizumab Tecentriq, PD-L1 Genentech MPDL3280A, RG7446, YW243.55.S70, RO5541267 avelumab Bavencio, PD-L1 Merck Serono, Pfizer MSB0010718C, A09- 246-2 AMP-224 PD-L1 Amplimmune, GSK, Medimmune cosibelimab CK-301, TG-1501 PD-L1 Checkpoint Therapeutics, Dana- Farber, Novartis, TG Therapeutics lodapolimab LY3300054 PD-L1 Lilly MCLA-145 4-1BB, PD-L1 Merus FS118 LAG-3, PD-L1 f-star, Merck Serono INBRX-105 ES101 4-1BB, PD-L1 Elpiscience, Inhibrx Suzhou PD-L1 Suzhou Nanomab Nanomab patent anti-PD- L1 MSB2311 PD-L1 Mabspace BCD-13 PD-L1 Biocad opucolimab HLX20, HLX09 PD-L1 Henlix IBI322 IBI-322 CD47, PD-L1 Innovent LY3415244 PD-L1, TIM-3 Lilly, Zymeworks GR1405 PD-L1 Genrix Biopharma LY3434172 PD-1, PD-L1 Lilly, Zymeworks CDX-527 CD27, PD-L1 Celldex FS222 4-1BB, PD-L1 f-star LDP PD-L1 Dragonboat Biopharma ABL503 4-1BB, PD-L1 ABL Bio HB0025 PD-L1, VEGF Huabo Biopharm MDX-1105 BMS-936559, 12A4 PD-L1 Medarex garivulimab BGB-A333 PD-L1 BeiGene GEN1046 4-1BB, PD-L1 BioNTech, Genmab NM21-1480 4-1BB, PD- Numab L1, Serum Albumin bintrafusp alfa M7824, PD-L1, TGFRII Merck Serono, NCI MSB0011359C pacmilimab CX-072 PD-L1 CytomX A167 KL-A167 PD-L1 Harbour Biomed Ltd., Sichuan Kelun Pharma IBI318 IBI-318 PD-1, PD-L1 Innovent, Lilly KN046 CTLA4, PD-L1 Alphamab STI-3031 IMC-001 PD-L1 Sorrento SHR-1701 PD-L1 Jiangsu Hengrui LP002 PD-L1 Taizhou HoudeAoke Bio STI-1014 ZKAB001 PD-L1 Lee's Pharm, Sorrento envafolimab KN035 PD-L1 Alphamab adebrelimab SHR-1316 PD-L1 Jiangsu Hengrui, Shanghai Hengrui CS1001 PD-L1 Cstone Pharma TQB2450 CBT-502 PD-L1 CBT Pharma, Chia Tai Tianqing Pharma

[0834] In some embodiments, the CTLA-4 inhibitor is selected from the group of inhibitors shown in Table 12.

TABLE-US-00012 TABLE 12 Exemplary CTLA4 Inhibitor Antibodies Name Internal Name Antigen Company ipilimumab Yervoy, MDX-010, CTLA4 Medarex MDX101, 10D1, BMS- 734016 ATOR-1015 ADC-1015 CTLA4, OX40 Alligator vudalimab XmAb20717 CTLA4, PD-1 Xencor SI-B003 CTLA4, PD-1 Sichuan Baili Pharma, Systimmune MGD019 CTLA4, PD-1 MacroGenics MEDI5752 CTLA4, PD-1 Medimmune ADU-1604 CTLA4 Aduro BCD-145 Q3W CTLA4 Biocad CS1002 CTLA4 Cstone Pharma REGN4659 CTLA4 Regeneron pavunalimab XmAb22841 CTLA4, LAG-3 Xencor AGEN1181 CTLA4 Agenus QL1706 PSB205 CTLA4, PD-1 Sound Biologics ADG126 CTLA4 Adagene KN044 CTLA4 Changchun Intelli-Crown ONC-392 CTLA4 OncoImmune, Pfizer BMS-986218 CTLA4 BMS BMS-986249 CTLA4 BMS BT-001 TG6030 CTLA4 BioInvent quavonlimab MK-1308 CTLA4 Merck (MSD) zalifrelimab AGEN1884 CTLA4 Agenus, Ludwig Inst., Sloan- Kettering AK104 CTLA4, PD-1 Akeso IBI310 IBI-310 CTLA4 Innovent KN046 CTLA4, PD-L1 Alphamab tremelimumab ticilimumab, CP- CTLA4 Amgen, Medimmune, Pfizer 675206, clone 11.2.1

[0835] In some embodiments, the immune checkpoint inhibitor is a small molecule drug. Small molecule checkpoint inhibitors are described, e.g., in WO2015/034820A1, WO2015/160641A2, WO2018/009505 A1, WO2017/066227 A1, WO2018/044963 A1, WO2018/026971 A1, WO2018/045142 A1, WO2018/005374 A1, WO2017/202275 A1, WO2017/202273 A1, WO2017/202276 A1, WO2018/006795 A1, WO2016/142852 A1, WO2016/142894 A1, WO2015/033301 A1, WO2015/033299 A1, WO2016/142886 A2, WO2016/142833 A1, WO2018/051255 A1, WO2018/051254 A1, WO2017/205464 A1, US2017/0107216 A1, WO2017/070089A1, WO2017/106634A1, US2017/0174679 A1, US2018/0057486 A1, WO2018/013789 A1, US2017/0362253 A1, WO2017/192961 A1, WO2017/118762 A1, US2014/199334 A1, WO2015/036927 A1, US2014/0294898 A1, US2016/0340391 A1, WO2016/039749 A1, WO2017/176608 A1, WO2016/077518 A1, WO2016/100608 A1, US2017/0252432 A1, WO2016/126646 A1, WO2015/044900 A1, US2015/0125491 A1, WO2015/033303 A1, WO2016/142835 A1, WO2019/008154 A1, WO2019/008152 A1, and WO2019023575A1.

[0836] In some embodiments, the PD-1 inhibitor is 2-[[4-amino-1-[5-(1-amino-2-hydroxypropyl)-1,3,4-oxadiazol-2-yl]-4-oxobutyl]carbamoylamino]-3-hydroxypropanoic acid (CA-170).

[0837] In some embodiments, the immune checkpoint inhibitor is(S)-1-(3-Bromo-4-((2-bromo-[1, l-biphenyl]-3-yl) methoxy)benzyl) piperidine-2-carboxylic Acid.

[0838] In some embodiments, the immune checkpoint inhibitor is a peptide. See, e.g., . Sasikumar et al., Peptide and Peptide-Inspired Checkpoint Inhibitors: Protein Fragments to Cancer Immunotherapy, Medicine in Drug Discovery 8:100073 (2020).

Variants

[0839] In some embodiments, the fusion protein(s) or components thereof described herein, or the NK cell genotypes described herein, are at least 80%, e.g., at least 85%, 90%, 95%, 98%, or 100% identical to the amino acid sequence of an exemplary sequence (e.g., as provided herein), e.g., have differences at up to 1%, 2%, 5%, 10%, 15%, or 20% of the residues of the exemplary sequence replaced, e.g., with conservative mutations, e.g., including or in addition to the mutations described herein. In preferred embodiments, the variant retains desired activity of the parent.

[0840] To determine the percent identity of two nucleic acid sequences, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). The length of a reference sequence aligned for comparison purposes is at least 80% of the length of the reference sequence, and in some embodiments is at least 90% or 100%. The nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position (as used herein nucleic acid identity is equivalent to nucleic acid homology). The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences.

[0841] Percent identity between a subject polypeptide or nucleic acid sequence (i.e, a query) and a second polypeptide or nucleic acid sequence (i.e, target) is determined in various ways that are within the skill in the art, for instance, using publicly available computer software such as Smith Waterman Alignment (Smith, T. F, and M. S. Waterman (1981) J Mol Biol 147:195-7); BestFit (Smith and Waterman, Advances in Applied Mathematics, 482-489 (1981)) as incorporated into GeneMatcher Plus, Schwarz and Dayhof (1979) Atlas of Protein Sequence and Structure. Dayhof. M.O., Ed, pp 353-358: BLAST program (Basic Local Alignment Search Tool: (Altschul, S. F., W. Gish, et al. (1990) J Mol Biol 215:403-10), BLAST-2, BLAST-P, BLAST-N, BLAST-X, WU-BLAST-2, ALIGN, ALIGN-2, CLUSTAL, or Megalign (DNASTAR) software. In addition, those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over the length of the sequences being compared. In general for target proteins or nucleic acids, the length of comparison can be any length, up to and including full length of the target (e.g., 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, or 100%). For the purposes of the present disclosure, percent identity is relative to the full length of the query sequence.

[0842] For purposes of the present disclosure, the comparison of sequences and determination of percent identity between two sequences can be accomplished using a Blossum 62 scoring matrix with a gap penalty of 12, a gap extend penalty of 4, and a frameshift gap penalty of 5.

[0843] Conservative substitutions typically include substitutions within the following groups: glycine, alanine: valine, isoleucine, leucine: aspartic acid, glutamic acid, asparagine, glutamine: serine, threonine: lysine, arginine; and phenylalanine, tyrosine.

Definitions

[0844] Unless defined otherwise, all terms of art, notations and other technical and scientific terms or terminology used herein are intended to have the same meaning as is commonly understood by one of ordinary skill in the art to which the claimed subject matter pertains. In some cases, terms with commonly understood meanings are defined herein for clarity and/or for ready reference, and the inclusion of such definitions herein should not necessarily be construed to represent a substantial difference over what is generally understood in the art.

[0845] Throughout this application, various embodiments may be presented in a range format. It should be understood that the description in range format is merely for convenience and brevity and should not be construed as an inflexible limitation on the scope of the disclosure. Accordingly, the description of a range should be considered to have specifically disclosed all the possible subranges as well as individual numerical values within that range. For example, description of a range such as from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numbers within that range, for example, 1, 2, 3, 4, 5, and 6. This applies regardless of the breadth of the range.

[0846] As used in the specification and claims, the singular forms a, an and the include plural references unless the context clearly dictates otherwise. For example, the term a sample includes a plurality of samples, including mixtures thereof.

[0847] The terms determining, measuring, evaluating, assessing, assaying, and analyzing are often used interchangeably herein to refer to forms of measurement. The terms include determining if an element is present or not (for example, detection). These terms can include quantitative, qualitative or quantitative and qualitative determinations. Assessing can be relative or absolute. Detecting the presence of can include determining the amount of something present in addition to determining whether it is present or absent depending on the context.

[0848] The terms subject, individual, or patient are often used interchangeably herein. The term in vivo is used to describe an event that takes place in a subject's body.

[0849] The term ex vivo is used to describe an event that takes place outside of a subject's body. An ex vivo assay is not performed on a subject. Rather, it is performed upon a sample separate from a subject. An example of an ex vivo assay performed on a sample is an in vitro assay.

[0850] The term in vitro is used to describe an event that takes places contained in a container for holding laboratory reagent such that it is separated from the biological source from which the material is obtained. In vitro assays can encompass cell-based assays in which living or dead cells are employed. In vitro assays can also encompass a cell-free assay in which no intact cells are employed.

[0851] As used herein, the term about a number refers to that number plus or minus 10% of that number. The term about a range refers to that range minus 10% of its lowest value and plus 10% of its greatest value.

[0852] As used herein, the term buffer solution refers to an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa.

[0853] As used herein, the term cell culture medium refers to a mixture for growth and proliferation of cells in vitro, which contains essential elements for growth and proliferation of cells such as sugars, amino acids, various nutrients, inorganic substances, etc.

[0854] A buffer solution, as used herein, is not a cell culture medium.

[0855] As used herein, the term bioreactor refers to a culture apparatus capable of continuously controlling a series of conditions that affect cell culture, such as dissolved oxygen concentration, dissolved carbon dioxide concentration, pH, and temperature.

[0856] The term vector, as used herein, refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. The term includes the vector as a self-replicating nucleic acid structure as well as the vector incorporated into the genome of a host cell into which it has been introduced. Some vectors are suitable for delivering the nucleic acid molecule(s) or polynucleotide(s) of the present application. Certain vectors are capable of directing the expression of nucleic acids to which they are operatively linked. Such vectors are referred to herein as expression vectors.

[0857] The term operably linked refers to two or more nucleic acid sequence or polypeptide elements that are usually physically linked and are in a functional relationship with each other. For instance, a promoter is operably linked to a coding sequence if the promoter is able to initiate or regulate the transcription or expression of a coding sequence, in which case, the coding sequence should be understood as being under the control of the promoter.

[0858] The terms host cell, host cell line, and host cell culture are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include engineered cells, transformants, and transformed cells, which include the primary engineered (e.g., transformed) cell and progeny derived therefrom without regard to the number of passages. Progeny may not be completely identical in nucleic acid content to a parent cell, but may contain mutations. Mutant progeny that have the same function or biological activity as screened or selected for in the originally transformed cell are included herein.

[0859] As appropriate, the host cells can be stably or transiently transfected with a polynucleotide encoding a fusion protein, as described herein.

[0860] The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described.

EXAMPLES

[0861] The following examples are included for illustrative purposes only and are not intended to limit the scope of the invention.

Example 1: Off-the-Shelf NK Cell Therapy Platform

[0862] One example of a method by which NK cells were expanded and stimulated is shown in FIG. 1.

[0863] As shown in FIG. 1, a single unit of FDA-licensed, frozen cord blood that has a high affinity variant of the receptor CD16 (the 158 V/V variant, see, e.g., Koene et al., FcRIIIa-158V/F Polymorphism Influences the Binding of IgG by Natural Killer Cell FcgammaRIIIa, Independently of the FcgammaRIIIa-48L/R/H Phenotype, Blood 90:1109-14 (1997).) and the KIR-B genotype (KIR B allele of the KIR receptor family, see, e.g., Hsu et al., The Killer Cell Immunoglobulin-Like Receptor (KIR) Genomic Region: Gene-Order, Haplotypes and Allelic Polymorphism, Immunological Review 190:40-52 (2002); and Pyo et al., Different Patterns of Evolution in the Centromeric and Telomeric Regions of Group A and B Haplotypes of the Human Killer Cell Ig-like Receptor Locus, PloS One 5: e15115 (2010)) was selected as the source of NK cells.

[0864] The cord blood unit was thawed and the freezing medium was removed via centrifugation. The cell preparation was then depleted of T cells using the QuadroMACS Cell Selection System (Miltenyi) and CD3 (T cell) MicroBeads. A population of 610.sup.8 total nucleated cells (TNC) were labelled with the MicroBeads and separated using the QuadroMACS device and buffer. Following depletion of T cells, the remaining cells, which were predominantly monocytes and NK cells, were washed and collected in antibiotic-free medium (CellgroSCGM). The cell preparation was then evaluated for total nucleated cell count, viability, and % CD3+ cells. The cord blood NK cells were CD3 depleted.

[0865] The CD3-cell preparation was inoculated into a gas permeable cell expansion bag containing growth medium. The cells were co-cultured with replication incompetent engineered HuT-78 (eHUT-78) feeder cells to enhance expansion for master cell bank (MCB) production. The CellgroSCGM growth media was initially supplemented with anti-CD3 antibody (OKT3), human plasma, glutamine, and IL-2.

[0866] The NK cells are optionally engineered, e.g., to introduce CARs into the NK cells, e.g., with a lentiviral vector, during one of the co-culturing steps.

[0867] The cells were incubated as a static culture for 12-16 days at 37 C., in a 5% CO2 balanced air environment, with additional exchanges of media occurring every 2 to 4 days. After the culture expanded more than 100-fold, the cultured cells were harvested and then suspended in freezing medium and filled into cryobags. In this example, 80 bags or vials were produced during the co-culture. The cryobags were frozen using a controlled rate freezer and stored in vapor phase liquid nitrogen (LN2) tanks below-150 C. These cryopreserved NK cells derived from the FDA-licensed cord blood unit served as the master cell bank (MCB).

[0868] To produce the drug product, a bag of frozen cells from the MCB was thawed and the freezing medium was removed. The thawed cells were inoculated into a disposable culture bag and co-cultured with feeder cells, e.g., eHUT78 feeder cells to produce the drug product. In this example, the cells are cultured in a 50 L bioreactor to produce thousands of lots of the drug product per unit of cord blood (e.g., 4,000-8,000 cryovials at 10.sup.9 cells/vial or 13,500 cryovials at 10.sup.8 cells/vial), which are mixed with a cryopreservation composition and frozen in a plurality of storage vessels, such as cryovials. The drug product is an off-the-shelf infusion ready product that can be used for direct infusion. Each lot of the drug product can be used to infuse hundreds to thousands of patients (e.g., 100-1,000 patients, e.g, with a target dose of 410.sup.9 cells or 110.sup.8 cells).

Example 2: Feeder Cell Expansion

[0869] As one example, suitable feeder cells, e.g., eHut-78 cells, were thawed from a frozen stock and expanded and cultured in a 125 mL flask in growth medium comprising RPMI1640 (Life Technologies), inactivated fetal bovine serum (FBS) (Life Technologies), and glutamine (Hyclone) at or at about 37 C., and at or at about 3-7% CO2. The cells were split every 2-3 days into 125 mL-2L flasks. The cells were harvested by centrifugation and gamma irradiated. The harvested and irradiated cells were mixed with a cryopreservation medium (Cryostor CS10) in cryovials and frozen in a controlled rate freezer, with a decrease in temperature of about 15 C. every 5 minutes to a final temperature of or of about90 C., after which they were transferred to a liquid nitrogen tank or freezer to a final temperature of or of about150 C.

[0870] After freezing, cell viability was greater than or equal to 70% of the original number of cells, and 85% or more of the cells expressed tmTNF-, 85% or more of the cells expressed mbIL-21+, and 85% or more of the cells expressed 4-1 BBL.

Example 3: NK Cell Expansion and Stimulation

[0871] As one example, suitable NK cells can be prepared as follows using HuT-78 cells transduced to express 4-1BBL, membrane bound IL-21 and mutant TNFalpha (eHut-78P cells) as feeder cells. The feeder cells are suspended in 1% (v/v) CellGro medium and are irradiated with 20,000 cGy in a gamma-ray irradiator. Seed cells (e.g., CD3-depleted PBMC or CD3-depleted cord blood cells) are grown on the feeder cells in CellGro medium containing human plasma, glutamine, IL-2, and OKT-3 in in static culture at 37 C. The cells are split every 2-4 days. The total culture time was 19 days. The NK cells are harvested by centrifugation and cryopreserved. Thawed NK are administered to patients in infusion medium consisting of:

[0872] Phosphate Buffered Saline (PBS 1, FujiFilm Irvine) (50% v/v), albumin (human) (20% v/v of OctaPharma albumin solution containing: 200 g/L protein, of which96% is human albumin, 130-160 mmol sodium: 2 mmol potassium. 0.064-0.096 mmol/g protein N-acetyl-DL-tryptophan, 0.064-0.096 mmol/g protein, caprylic acid, ad. 1000 ml water), Dextran 40 in Dextrose (25% v/v of Hospira Dextran 40 in Dextrose Injection, USP containing: 10 g/100 mL

[0873] Dextran 40 and 5 g/100 mL dextrose hydrous in water) and dimethyl sulfoxide (DMSO) (5% v/v of Avantor DMSL solution with a density of 1.101 g/cm3 at 20 C.).

[0874] In this example, the seed cells are CD3-depleted cord blood cells. A cell fraction can be depleted of CD3 cells by immunomagnetic selection. for example, using a CliniMACS T cell depletion set ((LS Depletion set (162-01) Miltenyi Biotec).

[0875] Preferably, the cord blood seed cells are selected to express CD16 having the V/V polymorphism at F158 (Fc gamma RIIIa-158 V/V genotype) (Musolino et al. 2008 J Clin Oncol 26:1789). Preferably, the cord blood seed cells are KIR-B haplotype.

[0876] Examples of two different manufacturing timelines are shown in FIG. 2. In one scheme, a master cell bank (MCB) is generated by stimulation of a NK cell source (e.g., a single cord blood unit) with feeder cells (e.g., el luT-78, as described herein) starting at day ( ) (DO), followed by transduction, e.g., with a vector comprising a CAR described herein, e.g., as described in Example 8, at Day 3 (D3), sorting, e.g., for CAR expression, at day 11 (D11), and harvesting and cryopreserving for a MCB at day 16 (D16). In another scheme, a MCB is generated by stimulation of a NK cell source (e.g., a single cord blood unit) with feeder cells (e.g., eHuT-78, as described herein) starting at day 9 (DO), followed by freezing & thawing of an intermediate at around day 7 (D7), transduction, e.g., with a vector comprising a CAR described herein, at around day 10 (D10), sorting and restimulation at around day 16 (D16) and harvesting for a MCB at about day 28 (D28). In some cases, a drug product (DP) is manufactured by thawing and stimulating a MCB (e.g., derived from one of the manufacturing timelines described above) with feeder cells (e.g., eHuT-78, as described here), starting at day 0) (DO), followed by bioreactor culturing at about day 8 (D8) and harvesting and cryopreserving for a drug DP at about day 14. In these examples, the initial NK: feeder cell ratio can be 1:2.5 and incubation can occur, for example as static culture at 37 C., in a 5% CO2 balanced air environment in a growth medium (for example, those described herein). Sorting can be carried out, for example, using an antibody specific for the CAR. The resulting cells can be frozen in a cryopreservation medium (for example, as described herein).

Example 4: Cord Blood as an NK Cell Source

[0877] NK cells make up five to 15% of peripheral blood lymphocytes. Traditionally, peripheral blood has been used as the source for NK cells for therapeutic use. However, as shown herein, NK cells derived from cord blood have a nearly ten-fold greater potential for expansion in the culture systems described herein than those derived from peripheral blood, without premature exhaustion or senescence of the cells. The expression of receptors of interest on the surface of NK cells, such as those involved in the activation of NK cells on engagement of tumor cells, was seen to be more consistent donor-to-donor for cord blood NKs than peripheral-blood NK cells. The use of the manufacturing process described herein consistently activated the NK cells in cord blood in a donor-independent manner, resulting in a highly scaled, active and consistent NK cell product.

Example 5: Expanded and Stimulated NK-Cell Phenotype

[0878] In one example NK cells from a cord blood unit are expanded and stimulated with eHut-78 cells, according to the expansion and stimulation process described in Example 1. As shown in FIG. 3, the resulting expanded and stimulated population of NK cells have consistently high CD16 (158V) and activating NK-cell receptor expression.

Example 6: CAR Costimulatory Structure Comprising OX40L

[0879] In some embodiments, the NK cells are CAR-NK cells. As shown in FIG. 4, CAR-NKs comprising a co-stimulatory domain comprising OX40L exhibited greater cytotoxic potential than those without OX40L. In this example, the CAR-NK cells comprise an anti-HER2 scFv as described in US20200399397A1, which is hereby incorporated by reference in its entirety.

[0880] In vitro efficacy, proliferation, CAR expression, and in vitro efficacy was compared for NK-CARs comprising the CARs with anti-HER2 scFy with (SEQ ID NO: 64) and without OX40L (SEQ ID NO: 66) (FIG. 5). As shown in FIG. 6 and FIG. 7, both CAR-NK structures proliferated in tumor negative control cells and expressed the CAR. In vitro efficacy (CD107a expression, cytokine production, and percent lysis) is shown for various cell lines (HER2 positive and trastuzumab sensitive target cells (SKBR3, NCI-N87, and SKOV-3). HER2 positive and trastuzumab resistant target cells (HCC1954), and HER2 negative target cells (MDA-MB-468) in FIG. 8, FIG. 9, FIG. 10, and FIG. 11). The OX40L containing CAR showed greater cytotoxic potential than that the CAR without OX40L against HER2 positive cell lines.

Example 7: AB-201

[0881] AB-201 is comprised of ex vivo expanded allogeneic cord blood derived natural killer (NK) cells that have been genetically modified to express a Human epidermal growth factor receptor 2 positive (HER2) directed chimeric antigen receptor (CAR) and IL-15 in a cryopreserved infusion ready suspension medium.

[0882] AB-201 is a cell suspension for infusion in buffered saline (with albumin, Dextran 40, and 5% DMSO), formulated as shown in Table 13.

TABLE-US-00013 TABLE 13 Components and Compositions of AB-201 Component Solution Quantity per Solution Composition Conc Conc Unit (11 mL fill) AB-201 drug Approximately 54% v/v 0.54 mL/mL 5.94 mL substance (ex 1 10.sup.7, 1 10.sup.8, or vivo- expanded 1 10.sup.9 viable cells allogeneic CAR- NK cells) PBS 100% Phosphate Buffered Saline (PBS) Albumin 250 g/L albumin in 16% v/v 40 mg/mL albumin 1.76 mL Solution water Dextran 40 100 g/L Dextran 40; 25% v/v 25 mg/mL Dextran 2.75 mL Solution and 50 g/L dextrose; 40; in water 12.5 mg/mL dextrose DMSO 100% DMSO (1,100 5% v/v 55 mg/mL 0.55 mL g/L)

Example 8: AB-201 Production

[0883] A CAR-NK expressing the fusion protein having SEQ ID NO: 59 was produced by transducing NK cells with a vector comprising SEQ ID NO: 61. The manufacture of AB-201 is conducted over a 2-stage process. Stage 1 produces the AB-201 master cell bank (identified as AB-201M), while stage 2 produces the AB-201 drug product (identified as AB-201P).

Example 9: CAR Constructs Expressing IL-15 have Increased Cytotoxicity

[0884] To investigate whether a CAR-NK which expresses both CAR and IL-15 has a synergistic effect on cytotoxicity, CAR-NK structures were generated as shown in FIG. 12.

[0885] NK cells including NK, mock-NK, CAR-NK, CAR (t)-IL-15-NK, and CAR-IL-15-NK (AB-201) group were generated from cord blood of a healthy donor. The CD3 negative cells in cord blood unit were purified by using CD3+ cells positive isolation kit, and then they were used as seed cells.

[0886] The seed cells included CD56+NK cells were stimulated with irradiated eHuT-78P cells and OKT3 and recombinant IL-2 (Proleukin) in complete serum-free medium (CellGro) on day 0.

[0887] The cultured NK cells were transduced by lentiviral vector on day 6 or 8 and were stimulated again with the irradiated eHuT-78P cells and OKT3 and IL-2 on day 14. At day 22. the cell groups were divided two groups again and cultured in the presence or absence of IL-2, respectively. Both transduced and non-transduced NK cells were cultured for 35 days in the presence of IL-2. As shown in FIG. 13, IL-15 secreting transduced expressed the CAR stably until day 35. As shown in FIG. 14, only IL-15 secreting transduced NK cells survived and expressed the CAR in the absence of IL-2. Moreover, the results show that expression of IL-15 increases the proportion of cells that are CAR+ in the absence of IL-2 (compare CAR-NK (43%) and CAR-IL-15-NK (91.3%) at day 29). As shown in FIG. 15, NK cells not secreting IL-15 did not proliferate after day 22 and, as shown in FIG. 16, their viability decreased rapidly after day 22. The results show that recombinant expression of IL-15 extends survival of NK cells even in the absence of IL-2.

[0888] To measure cytotoxicity, NK cells were cultured in the presence of IL-2 until day 22. and then cultured four more days in the absence of IL-2. At day 26, the NK cells were co-cultured with HCC1954 or SKOV3 at the E: T=0.3:1 ratio for long-term killing assay (FIG. 17) or E: T=1:1 ratio for IFNg ELISA (FIG. 18) for 6 days in the absence of IL-2. CAR-IL-15-NK cells had a higher cytotoxicity than that of other NK cells. These results show that the CAR comprising an OX40L costimulatory domain and IL-15 expression exhibited better and more sustained killing activity. The CAR-NK cells lacking IL-15 expression showed significantly reduced killing activity compared to the cells expressing IL-15 under these conditions. On day 32, the amount of IFNg in the culture supernatant was measured. The CAR-IL-15-NK cells produced the highest amount of IFNg, and it was correlated to the cytolytic activity results in FIG. 17.

[0889] To measure IL-15 production, the non-transduced or transduced NK cells were cultured in the presence of IL-2 until day 28, and then cultured four more days in the absence of IL-2. At day 32, the indicated NK cells were co-cultured with HCC1954 or SKOV3 for 72 hours in the absence of IL-2, and the IL-15 levels in the cultured supernatant were determined by ELISA, as shown in FIG. 19. These results show that co-culturing CAR-IL-15-NK cells in the presence of HER2+ target cells increased the amount of IL-15 produced by the NK cells. In contrast, the CAR (t)-IL-15-NK cells without costimulatory domains generated relatively constant amounts of basal IL-15 expression in both the absence of and presence of target cells. NK cells that lacked recombinant nucleic acids encoding IL-15 did not generate significant levels of IL-15 expression.

Example 10: Secretion of IL-15 Maintains the Survival of Bystander NK Cells

[0890] NK cells and CAR-IL-15-NK (AB-201) cells were generated from two different donors. Cells were transduced at day 8 to produce CAR-IL-15-NK. At day 14, the NK cells were re-stimulated and CAR-IL-15-NK cells were re-stimulated and sorted. At day 19, the NK cells were CFSE labeled and co-cultures were created by mixing CFSE NK cells and CAR-IL-15-NK cells at a 1:1 ratio. Cocultures either with or without IL-2 were carried out for 5 days. Fixable viability dye (Invitrogen #65-0865) was used to detect viable cells. As shown in FIG. 20, despite the absence of IL-2, the frequency of living NK cells that co-cultured with CAR-IL-15-NK cells was not decreased in the experiments using two different donors.

Example 11: Long Term Stability and Survival of CAR-NKs Expressing IL-15

[0891] NK cells expressing CARs with and without IL-15 (FIG. 21) were cultured as described in Example 10 to day 19. At day 19, they were cultured without IL-2. As shown in FIG. 22, the CAR-expressing cells lacking IL-15 (3rd CAR) had significantly reduced CAR expression levels (e.g., only 55.2% of max at day 30) as compared to cells expressing the CAR with IL-15 (4th CAR) (e.g., 97.1% of max at day 56). The CAR-expressing cells lacking IL-15 (3rd CAR) also failed to persist as long, as none survived until day 44. As shown in FIG. 23 and FIG. 24, the cells expressing the CAR lacking IL-15 did not survive past day 37, whereas the cells expressing the CAR with IL-15 survived at least up to day 62, and also maintained viability. As shown in FIG. 25, cells expressing IL-15 persisted better than cells lacking heterologous expression of IL-15 in the presence target cells.

Example 12: AB-201 In vitro Studies

Characterization of AB-201

[0892] Purity and phenotype of AB-201 was evaluated by flow cytometry. NK cell maturation is determined through expression of the markers, CD56 and CD16 while NK cell activity and regulation are conferred through a balance of activating and inhibitory receptor expression. The expression pattern of these receptors was determined by flow cytometry using receptor-specific reagent antibody staining. Cord blood NK (CB-NK) cells were used as a control.

[0893] AB-201 purity and identity was determined through the assessment of surface markers CD56, CD3, CD14, and CD19. CD56 is the archetypal phenotypic marker of natural killer maturation whereas CD3, CD14, and CD19 are markers for T cells, monocytes, and B cells, respectively. Expression of CD16 (FcRIII) is also an indicator of the NK cell maturation state (FIG. 31). Further, CD3, CD14, and CD19 constitute 0% of the AB-201 cell population (FIG. 31). Further characterization of AB-201 demonstrated high expression of activating receptors such as NKG2D, NKp30, NKp46, and DNAM-1 and expression of the chemokine receptor. CXCR3 (FIG. 32). The mean number of cells in the AB-201 sample that expressed the CAR was 92.5%.

Killing Activity of AB-201

[0894] Cytotoxicity of AB-201 against tumor cell lines was assessed using short (4 hr) and long-term (up to 5 day) assays. Cytotoxicity of NK cells can be quantitatively measured at a range of NK cell (effector) to tumor cell (target) ratios. Target cells included SKOV-3, HCC1954, and NCI-N87, HER2+ cancer cell lines of ovarian, breast, and gastric origin, respectively.

[0895] AB-201 demonstrated concentration-dependent cytotoxic activity against the tumor cell lines SKOV-3, HCC1954, and NCI-N87 (FIG. 33). Cytotoxic activity of AB-201 was greater than the donor-matched, non-engineered, eHuT-78-expanded cord-blood derived NK cells (CBNK) in all the cell lines tested. These results indicate that AB-201 has potent cytotoxic activity against HER2+ cancer cell lines.

[0896] Long-term cytotoxicity assays were performed using an Incucyte Live-Cell analysis system which images the NK and target cell co-culture over time.

[0897] AB-201 demonstrated potent long-term cytotoxic activity against SKOV-3, HCC-1954, and NCI-N87 cancer cell lines over the 5 day timeframe (FIG. 34). In co-cultures with SKOV-3 or HCC-1954 the cytotoxic activity of AB-201 exceeds that observed with non-engineered CBNK cells. When AB-201 is co-cultured with NCI-N87 cells the differential between AB-201 and CBNK cells is less; however, the NCI-N87 experiment was performed utilizing phase contrast analysis of tumor cell confluence which could be a less sensitive measure in this system.

[0898] FIG. 26 shows in vitro killing activity of AB-201 against the breast carcinoma (HER2-) cell line MDA-MB-468.

[0899] FIG. 27 shows in vitro killing activity of AB-201 against the ovarian carcinoma (HER2+) cell line SKOV3.

[0900] FIG. 28 shows in vitro killing activity of AB-201 against the gastric carcinoma (HER2+) cell line NCI-N87.

[0901] FIG. 29 shows in vitro killing activity of AB-201 against the breast carcinoma (HER2+) cell line HCC1954.

[0902] FIG. 30 shows in vitro killing activity of AB-201 against the breast carcinoma (HER2+) cell line K562.

Intracellular Cytokine Staining. Marker of Degranulation and Cytokine Secretion

[0903] AB-201 cells were co-cultured with target tumor cells (K562, an immortalized myelogenous leukemia cell line that is widely used in NK cell cytotoxicity assessments, SKOV-3, HCC1954 and NCI-N87). Golgi-plug and Golgi-stop were used to prevent extracellular secretion of cytokine and CD107a. Production of intracellular cytokines and expression of degranulation markers by AB-201 in response to stimulation with tumor cells was measured by flow cytometry. Cytokine secretion in response to co-culture of AB-201 with target tumor cells (SKOV-3, HCC1954, and NCI-N87) was assessed by ELISA.

[0904] In line with cytotoxic activity, co-culturing of AB-201 with various cancer cell lines (K562, SKOV-3, HCC1954. And NCI-N87) resulted in increased production of effector cytokines (IFN-. TNF) and expression of a marker of degranulation (CD107a) over that observed with the NK cells alone (no target control) (FIG. 35). Further, there was an enhancement of INF-y and TNFa production and CD107a expression in NK cells co-cultured with HER2+ cancer cell lines compared to the non-engineered CBNK cells. This enhancement was not observed with the non-HER2-expressing K562 cancer cell line. In co-culture with K562 the AB-201 results are more comparable to the non-engineered CBNK cells. These results confirm AB-201 activity in response to co-culture with HER2+ tumor cells.

[0905] Cytokine levels (INF-y and IL-15) were assessed in the culture media following co-culture of AB-201 with SKOV-3, HCC1954, and NCI-N87 cancer cell lines. Consistent with the intracellular cytokine staining results, an x-y fold elevation in INF-y was observed with AB-201 compared to non-engineered CBNK cells. This enhanced increase in the presence of HER2+ cancer cells was also observed with IL-15 when co-cultures with AB-201 were compared to the non-engineered CBNK and no target control indicating specific activation by cancer cells expressing the HER2 target. FIG. 36.

[0906] As shown in FIG. 37, the growth of a human HER+ gastric carcinoma cell line. NCI-N87, was monitored by measuring the cell confluence in long term cultures. Trastuzumab was seen to inhibit growth of the culture, whereas trastuzumab combined with the non-CAR NK product AB-101, resulted in further depletion of cell confluence via ADCC cell killing. It was shown that AB-201 had significantly greater cytotoxic killing activity over trastuzumab in combination non-CAR NKs.

[0907] HER2-Dependent Cytotoxicity on Primary Cells

[0908] Cytotoxicity of primary cells (non-tumor) was measured following co-culture of AB-201 or control CB-NK cells with pulmonary artery endothelial cells, keratinocytes, renal epithelial cells, cardiac myocytes and small airway epithelial cells for 4 hours at Effector: Target (E: T) ratios of 3:1. 1:1, or 0.3:1. No HER2-dependent cytotoxicity was observed (FIG. 44).

Example 13: AB-201 In vivo Studies

[0909] In vivo efficacy of AB-201 has been evaluated in murine xenograft models bearing HER2+ tumors includingHCC1954, SKOV-3, and NCI-N87.

HCC1954

[0910] AB-201 demonstrated anti-tumor efficacy in a the human HCC1954 mouse xenograft model of breast cancer using the human HCC1954 breast cancer cell line, which has been characterized as trastuzumab resistant. HCC1954-luc tumor cells were grown in cell culture, harvested, and concentrated to 510.sup.6 cells/mL with PBS (phosphate buffered saline). Mice were injected intraperitoneally (IP) with 110.sup.6 cells/mouse. As shown in FIG. 39, tumors were established in the mice, which were imaged on day zero.

[0911] Three days after HCC1954-luc inoculation, mice were randomized to one of 7 groups (Table 14) according to bioluminescence of Day 0 (average bioluminescence signal was 2.49E08 photons/s). AB101, AB201, TRZ and IL-2 were administered intraperitoneally.

TABLE-US-00014 TABLE 14 Volume No. of Group Dose Route (L) Animals 1 Freezing medium + IgG 5 i.p + i.p 200 + 200 8 mg/kg 2 TRZ 5 mg/kg i.p 200 8 3 AB101 2 10.sup.7 cells + IL-2 i.p + i.p 200 + 100 8 4 AB101 + TRZ + IL-2 i.p + 200 + 200 + 8 i.p + i.p 100 5 AB201 2 10.sup.7 cells + IL-2 i.p + i.p 200 + 100 8 6 AB201 5 10.sup.6 cells + IL-2 i.p + i.p 200 + 100 8 7 AB201 1 10.sup.6 cells + IL-2 i.p + i.p 200 + 100 8

[0912] A single dose of five million AB-201 cells was administered on day four in the appropriate groups. All animals were observed for general symptoms and death two times a day (once a day for weekends and holidays) during the study period. All animals were weighed three times a week. Bioluminescence imaging was performed 8 times (Day 0, 7, 14, 19, 25, 31, 38, 45) using IVIS Spectrum in vivo imaging system (PerkinElmer).

[0913] As shown in FIG. 38, survival rates were highest for AB-201, followed by AB-101 with trastuzumab. 87.5% of animals receiving AB-201 were still alive at the end of the experiment. Trastuzumab increased median survival time by 10.5 days and AB-101+ trastuzumab increased median survival time by 38.5 days.

[0914] As shown in FIG. 39, AB-201 led to tumor suppression by day seven, and in four out of the five mice complete tumor regression, with no recurrence through the duration of the study (Day 45). The bioluminescence signal of the Vehicle group increased steadily over the course of the study. Compared to the vehicle group, the bioluminescence of AB101+IL-2, AB101+ TRZ +IL-2, and AB201+IL-2 groups decreased by 78.3%, 59.3%, 97.2% and 81.9% at day 45, respectively. Each of these decreases had a p value of less than 0.05.

[0915] The results show that AB-201 performed substantially better at controlling HCC1954 tumors than trastuzumab

[0916] SK-OV-3

[0917] AB-201 demonstrated substantial tumor regression and survival benefits in the SK-OV-3 human ovarian cancer cell line xenograft model system. Three administrations of AB-201 conferred a significant survival benefit (FIG. 40).

[0918] In a separate experiment. NSG mice received 110.sup.6 SKOV3-Luc tumor cells (IP) on day 0 and a single injection of AB-201 (IP) on day 11Bioluminescence (BLI) measurements of SKOV3-Luc, mean total flux+SEM for each group of mice are shown in FIG. 45. The difference in tumor volume between the AB-201 and untreated mice had a p value of <0.0001 as determined by two-way ANOVA (noted as **** in FIG. 45). Differences in body weight were not observed over the course of the study (FIG. 46).

[0919] AB-201's ability to persist in NSG mice was also assessed. Blood samples were obtained on day 52. AB-201 cells were still detectable by flow cytometry when gating for human CD45+/CD56+ cells (FIG. 47).

NCI-N87

[0920] AB-201 demonstrated substantial tumor regression and survival benefits in the NCI-N87 human gastric carcinoma cell line xenograft model system.

[0921] Two administrations of AB-201 confers significant survival benefit and tumor regression. Mice were injected as shown in FIG. 41. FIG. 42 shows percent survival. FIG. 43 shows tumor load.

[0922] In a separate experiment, 30 female mice aged six weeks were inoculated subcutaneously in their right flanks with 110.sup.7 NCI-N87 cells/mouse on day 0. Some of the mice also received a single dose of 1.5 Gy (150 rad) of full body irradiation on day-1. Mice were left untreated, administered a single dose of 510.sup.6 AB-201 cells/mouse intravenously, or administered a single dose of 510.sup.6 cord blood NK (CB-NK) cells/mouse intravenously on day 5 post-tumor implantation. Mice were observed daily and tumor volume and body weight were measured twice a week. Mice were euthanized when the No Treatment control group (Group 1) mean tumor volume reached >500 mm.sup.3. The study ended on Day 53.

[0923] AB-201 demonstrated significant efficacy over no treatment and CB-NK (P<0.0001, Two-way ANOVA) in irradiated mice (FIG. 48) and unconditioned mice (FIG. 49). AB-201 was well tolerated based on no change in body weight across groups (FIG. 50).

[0924] At study end, all tumor bearing mice in all groups were euthanized and tumors were excised, and wet weights were recorded in the necropsy inventory. Slits were cut in the tumor every 3-5 mm (to ensure full penetration of fixative), placed in 10% neutral buffered formalin (VWR: Radnor, PAK) for 48-72 hours then transferred to 70% ethanol (EMD Millipore: Billerica, MA) and stored at room temperature.

[0925] Tissues were assessed for AB-201 infiltration. Samples were trimmed, processed, and embedded as formalin-fixed paraffin embedded blocks. Blocks were then sectioned at 4 m onto positively-charged slides. Immunofluorescence was performed using a rabbit-anti-CD56 antibody. Heat induced antigen retrieval was performed using Leica Bond Epitope Retrieval Buffer 1 (Citrate solution, pH6.0) for 20 minutes. Non-specific background was blocked with Novocastra Protein Block (Leica, #RE7102-CE, Lot #6055249) for 20 minutes. Primary antibody was applied for overnight incubation at 4 C. A Goat anti-Rabbit IgG Alexa Fluor Plus 647 (red) at a dilution of 1:200 (ThermoFisher, #A31573, Lot #1964354) was applied for 60 minutes at room temperature. Slides were mounted with DAPI in Fluorogel II for nuclear visualization (blue). FIG. 51 depicts representative images of H&E, HER2 IHC and CD56 immunofluorescence staining of tumor section from mice receiving no treatment, CBNK or AB-201. HER2 immunohistochemistry staining on tumor section (10magnification), staining visualized in DAB (dark) and hematoxylin was used as nuclear counterstain (blue). CD56 is indicated in red (right) and DAPI used as a nuclear counterstain in blue (40magnification). The scale bar represents 50 m. The DC56 staining results demonstrate that AB-201 infiltrated the tumor.

Example 14: Further In vivo Studies

[0926] Here we tested the anti-tumor activity of AB-201, an ex vivo-expanded allogeneic cord blood-derived natural killer cell (CB-NK) that has been genetically modified to express a HER2-directed chimeric antigen receptor (CAR), in a HER2-expressing mouse xenograft model.

[0927] Efficacy of AB-201 against HER2+ tumors was evaluated in an NSG mouse (Jackson Laboratories: Bar Harbor, ME) xenograft using the SKOV-3 ovarian adenocarcinoma model. SKOV-3 cells were obtained from ATCC (American Type Culture Collection, Manassas, VA). The SKOV-3 cell line was modified to stably express a luciferase gene (SKOV-3-Luc) which allows for noninvasive bioluminescent imaging (BLI) of tumor cells in vivo in the presence of the substrate d-luciferin. Female NSG mice (n=8/group) were inoculated with 110.sup.6 SKOV-3-Luc tumor cells intraperitoneally on Day 0. On day 4, animals were randomized into seven groups based on tumor burden as defined by BLI signal. Two doses of AB-201 (110.sup.6 and 510.sup.6) were tested in this study and both dose levels were administered IP as either a single administration or as two administrations in tumor-bearing NSG mice starting on day 5 post-tumor inoculation, with the second administration on day 12. Donor-matched cord blood natural killer cells (CB-NK) were administered as a single dose of 510.sup.6 cells/mouse as a control for tumor cell sensitivity to non-engineered NK cells. Tumor cells alone serve as the no treatment control.

[0928] Tumors were measured by IVIS starting on Day 4 (once a week). The mice were injected subcutaneously (s.c.) with 150 mg/kg d-Luciferin 15 minutes prior to imaging. Mice were anaesthetized and placed into the imaging chamber (Spectrum CT) ten minutes following administration of d-Luciferin and imaged for luminescence. The BLI is measured in photons/see and is expressed as total flux.

[0929] In the intraperitoneal SKOV-3-Luc xenograft model, anti-tumor efficacy of AB-201 was observed at both dose levels tested. AB-201 administration decreased the SKOV-3-Luc tumor burden as assessed by the BLI signal on day 52. Compared to the no treatment control and CB-NK group, AB-201 demonstrated delayed tumor progression as evidenced by reduced luciferase signal (p<0.0001 all AB-201groups). AB-201 administered as a single dose at either 110.sup.6 or 510.sup.6 cells/animal compared to the no treatment control, demonstrated an 82.8% and a 95.6% decrease in tumor burden, respectively. Further, when compared to the CB-NK control group, a 79.4% and 94.8% decrease in tumor burden was observed following a single administration of AB-201 at 110.sup.6 or 510.sup.6, respectively, on day 52. Similar data was obtained with the multiple dose groups. AB-201 was well tolerated at both dose levels with no significant body weight (BW) changes associated with treatment across any of the groups. All animals that received AB-201 survived the duration of study, while four animals in the no treatment and one animal that received CB-NK were found dead or were a moribund sacrifice. AB-201 was detected in peripheral blood on day 7 (2 days post-NK cell administration), day 14 (2 days post-additional administration for AB-201 groups 6 &7), day 21 and at term analysis on day 62, while CB-NK detection peaked early on day 7 and gradually decreased and each subsequent timepoint indicating that both CB-NK and AB-201 were capable of trafficking from the site of injection into the periphery. In conclusion, these results demonstrate significant anti-tumor efficacy of AB-201 in an ovarian SKOV-3 xenograft tumor model and suggest the therapeutic potential of AB-201 against HER2+ tumors.

[0930] Spleen and blood samples were collected, processed, and stained for the flow cytometry analysis with a 4-color panel as shown in Table 15 below.

TABLE-US-00015 TABLE 15 Flow Cytometry Antibodies Markers Fluorochrome Clone Cat # Isotype Vendor hCD45 AF700 HI30 304024 Mouse IgG1, BioLegend hCD56 BUV395 NCAM16.2 563554 Mouse IgG2b, BD hCD16 FITC 3G8 302006 Mouse IgG1, BioLegend L/D efluo780 n/a 65-0865-18 n/a eBioscience

[0931] As shown in FIG. 53, BLI measurements of SKOV-3-Luc tumor xenografts were taken at multiple timepoints following administration of tumor cells, mean total [flux photons/second]SEM for each group of mice is shown. **** p<0.0001, two-way ANOVA with Tukey's multiple comparison test.

[0932] AB-201 tolerability was determined by group percent BW change and is shown in FIG. 54. AB-201 was well tolerated at both dose levels of 110.sup.6 cells/mouse and 510.sup.6 cells/mouse. There was observed body weight gain in the AB-201-treated groups and weight loss in the untreated or CB-NK treated groups by day 55 of study. No adverse events were observed in any AB-201-treated animals in this study. All animals receiving AB-201 survived the duration of the study. Only animals from the no treatment and CB-NK control groups were found dead or sacrificed due to tumor burden.

[0933] As NK cells were administered by IP injection, we wanted to determine whether there was trafficking of NK cells into the periphery. Peripheral blood was assessed at multiple timepoints post-NK cell infusion and at term by flow cytometry (FIG. 55). AB-201 was detected in peripheral blood on day 7 (2 days post-NK administration), day 14 (2 days post-additional administration for AB-201 group 6 &7), day 21 and at term analysis on day 62 while CB-NK detection peaked early on day 7 and gradually decreased and each subsequent timepoint. These data demonstrate that AB-201 persisted for at least 57 days following a single injection (both dose levels). In addition, at term collection AB-201 was detected in spleen with higher presence correlating with dose level. These data indicate that both CB-NK and AB-201 were capable of trafficking from the site of injection into the periphery.

[0934] Efficacy of AB-201 was observed at 110.sup.6 or 510.sup.6 dose levels following a single dose administration on Day 5 or multiple doses administered on Days 5 and 12 post-tumor cell inoculation in the SKOV-3 ovarian carcinoma tumor model. AB-201 was detected in peripheral blood and spleen at term on day 62, indicating trafficking to the periphery in a tumor bearing animal. Both AB-201 dose levels (110.sup.6 or 510.sup.6 cells/mouse) administered either as single or multiple doses were well tolerated as demonstrated by body weight gain in the AB-201-treated groups and weight loss in the untreated and CB-NK treated groups by day 55 of study. In addition, no adverse events were observed in any AB-201 treated animals in this study. In conclusion, these AB-201 data demonstrate significant in vivo anti-tumor activity and good tolerability in the SKOV-3 xenograft model.

Example 15: Further In vitro studies

[0935] AB-201 consists of ex vivo-expanded allogeneic cord blood-derived natural killer cells that have been genetically modified to express a HER2-CAR. This study characterizes the purity, NK cell activity, phenotypic characteristics (i.e., expression of inhibitory receptors), and cytotoxicity and cytokine secretion against tumor cells of AB-201.

[0936] The purity of NK cells was determined through CD3-CD56+expression. A high purity of 98.70.8% (meanSD) CD3-CD56+expressing cells was observed in the final AB-201 drug product. In addition, AB-201 demonstrated high expression levels of NK activating receptors (i.e., CD16. NKG2D. NKp30, NKp46, and DNAM-1) and chemokine receptors (i.e., CXCR3).

[0937] The short-term (4 hr) cytotoxicity assay was performed to confirm the direct tumor cell killing activity of AB-201. The cytotoxicity against tumor cell lines was compared between AB-201 cells and donor matched, eHuT-78-expanded CBNK incubated with tumor cells at different Effector: target (E: T) ratios. According to the short-term cytotoxicity analysis results, it was confirmed significant cytotoxicity against HER2-positive tumor cell lines (i.e., SKOV-3, HCC1954, and NCI-N87) was observed for AB-201 in comparison to CBNK at an ET ratio of 10:1.

[0938] The long-term (5 days) cytotoxicity assay was performed to confirm the direct tumor cell killing activity of AB-201. The long-term cytotoxicity assay evaluates NK cell killing activity through a 5-day measurement of fluorescence reduction following co-culture with tumor cell lines expressing red fluorescent protein. Results indicate a higher tumor cell killing activity for AB-201 compared to CBNK as the co-culture of SKOV-3 cells with CBNK resulted in 95.72.5% tumor cells while co-culture of SKOV-3 cells with AB-201 resulted in 33.34.5% tumor cells. Similarly, a larger number of HCC1954 tumor cells was reduced when co-cultured with AB-201 as compared to co-culture with CBNK (82.96.1% vs 25.3=1.0% tumor cells for CBNK or AB-201, respectively). Regarding the NCI-N87 tumor cell line which does not express fluorescent proteins, the degree of cell killing is assessed through the confluence of the tumor cells. A larger decrease in tumor cell confluence was achieved by AB-201 as 54.56.9% and 38.84.2% tumor cells were measured for CBNK and AB-201, respectively at the end of the 5 day period. Based on these results, AB-201 demonstrated enhanced anti-tumor activity against HER2tumor cell lines.

[0939] The NK cell activity of AB-201 was evaluated through cytokine secretion and CD107a expression. After co-culture of NK cells (CBNK orAB-201) with HER2-positive tumor cell lines, cytokine secretion and CD107a expression levels were assessed. Compared to CBNK. a 4 to 6-fold expression of CD107a, 2 to 4-fold expression of IFN-, and 2 to 4-fold expression of TNF- was observed for the AB-201.

[0940] To confirm IL-15 secretion, which is relevant to persistence of CAR-NK cells in vivo, AB-201 was co-cultured with HER2-positive tumor cell lines, and the IL-15 level within the culture medium was measured. Results confirmed a 3.5 to 6-fold higher IL-15 secretion of AB-201 compared to CBNK.

[0941] In summary, AB-201 demonstrated enhanced anti-tumor activity in a HER2-dependent manner.

Cell Viability

[0942] The cell viability of AB-201 post thaw was measured in three separate experiments performed on different days using an automated cell counter (ADAM cell counter). Donor matched CBNK was included as a control. The average cell viability of AB-201 and CBNK post-thaw was 97.3+0.7% (meanSD) and 91.8+3.9% respectively, indicating high viability for both.

Purity

[0943] The purity of AB-201 and CBNK cells was assessed using FACS. The content of NK cells (CD3 CD56+) , T cells (CD3+), monocytes (CD14+), and B cells (CD19+) were measured in three separate experiments for both AB-201 and CBNK.

[0944] Purity results for CBNK and AB-201 confirmed that the content of CD3 CD56+NK cells was 98.64+0.33% and 98.65+0.83%, respectively. CD3+ T cells were not detected in CBNK and AB-201, CD14+ monocytes were 0.01+0.02% and 0.06+0.10%, respectively, and CD19+B cells were 0.49+0.17% and 0.12+0.12%, respectively. In summary, these results demonstrate high purity of NK cells in AB-201.

NK Cell Phenotypes

[0945] Activation/inhibitory receptors, chemokine receptors, and surface molecules related to cytotoxicity are expressed on the surface of NK cells. To confirm the expression of these molecules, the cell phenotype of CBNK and AB-201 was analyzed using FACS.

[0946] The activation receptors that were highly expressed at an average of at least 90% in both CBNK and AB-201 include CD16, NKG2D, NKp30, and DNAM-1. The inhibitory receptor NKG2A was also highly expressed at an average of at least 90%. In particular, a higher average expression of 20% was observed for NKp46 in AB-201 compared to CBNK. Similarly, a higher average expression of 30% was observed for chemokine receptor, CXCR3 (FIG. 56). HER2 CAR Expression

[0947] The content of HER2 CAR expressed in AB-201 was analyzed using FACS. The result of thawing AB-201 and measuring the HER2 CAR expression confirmed an average of 90.42.9%.

Short Term Cytotoxicity

[0948] K562: To evaluate the anti-tumor activity of AB-201 against K562 at several E: T ratios (10:1, 3:1, 1:1, 0.3:1) were measured in three separate experiments. At a 10:1 E:T ratio, CBNK and AB-201 yielded measurements of 71.42.8% and 74.41.1%), respectively. The CBNK and AB-201 cytotoxicity was measured to be 57.56.4% and 67.12.1% at an E:T ratio of 3:1, 31.43.8% and 38.73.3% at E:T ratio of 1:1, and 12.11.6% and 15.12.9% at an E: T ratio of 0.3:1. respectively. Hence, an E:T ratio-dependent anti-tumor activity with no significant differences between the CBNK and AB-201 was observed (FIG. 57, Table 16).

[0949] SKOV-3: The anti-tumor activity of AB-201 was evaluated against SKOV-3, which is a HER2+ovarian cancer cell line, in three separate experiments. At an ET ratio of 10:1, CBNK showed a cytotoxicity of 35.45.7%, while AB-201 demonstrated a cytotoxicity of 57.45.6%. The cytotoxicity for the CBNK and AB-201 was measured to be 18.9+2.2% and 32.56.2% at an E:T ratio of 3:1, 9.72.2% and 14.2+2.7% at an E:T ratio of 1:1, and 4.0=1.1% and 2.12.2%, at an E:T ratio of 0.3:1, respectively. These results demonstrated HER2-dependent anti-tumor activity of AB-201 that was statistically significant in comparison to CBNK (at an E:T ratio of 10:1. ** p<0.01, at an E:T ratio of 3:1, *p<0.05, at an E:T ratio of 1:1, ** p<0.01, two-tailed t-test) (FIG. 57, Table 16).

[0950] HCC1954: The anti-tumor activity of AB-201 was evaluated against HCC1954, a HER2+ breast cancer cell line in three separate experiments. At an E:T ratio of 10:1, cytotoxicity for CBNK was 27.9+9.5% while AB-201 mediated cytotoxicity was 71.53.5% (statistically significant difference p<0.05, two-tailed t-test). Similarly, at an E:T ratio of 3:1, the measured cytotoxicity for CBNK and AB-201 was 13.3+4.6% and 46.13.3%, respectively, which demonstrated that the anti-tumor activity of AB-201 is significantly higher (p<0.05, two-tailed t-test) than that of CBNK. At an E:T ratio of 1:1, AB-201 exhibited significantly greater cytotoxicity compared to CBNK (E: T ratio of 1:1, *p<0.05, two-tailed t-test) (FIG. 57, Table 16).

[0951] NCI-N87: The anti-tumor activity of AB-201 was evaluated against NCI-N87, a HER2+ gastric carcinoma cell line in three separate experiments. At an E:T ratio of 10:1, the cytotoxicity of CBNK was 32.916.8% while AB-201 mediated cytotoxicity was 60.74.7%. At an E: T ratio of 3:1, the cytotoxicity of CBNK and AB-201 cells was observed to be 19.3+8.4% and 39.44.9%, and 12.79.8% and 20.18.8% respectively at an E:T ratio of 1:1. At an E: T ratio of 0.3:1, CBNK was 8.110.3% and AB-201 was 9.49.8%. Hence, results demonstrated that the anti-tumor activity of AB-201 was statistically significant compared to CBNK except for the E: T ratio of 0.3:1 (at an ET ratio of 10:1, *p<0.05, at an ET ratio of 3:1, *p<0.05, at an E: T ratio of 1:1, *p<0.05, two-tailed t-test) (FIG. 57, Table 16).

TABLE-US-00016 TABLE 16 Summary of direct cytotoxicity of CBNK, AB-201 against tumor cells (n = 3) Target cell: K562 Target cell: SKOV-3 Specific lysis (%) CBNK AB-201 CBNK AB-201 E:T ratio Mean SD Mean SD Mean SD Mean SD 10:1 71.4 2.8 74.4 1.1 35.4 5.7 57.4 5.6 3:1 57.5 6.4 67.1 2.1 18.9 2.2 32.5 6.2 1:1 31.4 3.8 38.7 3.3 9.7 2.2 14.2 2.7 0.3:1 12.1 1.6 15.1 2.9 4.0 1.1 2.1 2.2 Target cell: HCC1954 Target cell: NCI-N87 Specific lysis (%) CBNK AB-201 CBNK AB-201 E:T ratio Mean SD Mean SD Mean SD Mean SD 10:1 27.9 9.5 71.5 3.5 32.9 6.8 60.7 4.7 3:1 13.3 4.6 46.1 3.3 19.3 8.4 39.4 4.9 1:1 5.8 2.5 22.0 3.3 12.7 9.8 20.1 8.8 0.3:1 1.7 0.6 6.9 1.9 8.1 10.3 9.4 9.8

Long Term Cytotoxicity

[0952] To test the long-term anti-tumor activity of CBNK and AB-201 against HER2-expressing target cells, CBNK and AB-201 were co-cultured with NCI-N87 and red fluorescent protein-expressing HCC1954 and SKOV-3 cell lines for 5 days.

[0953] After co-culturing the target cell lines with NK cells, the anti-tumor activity against the NCI-N87 cell line, which does not express the red fluorescent protein, was determined by confluence (%). The anti-tumor activity against HCC1954 and SKOV-3 cells was measured by the fluorescence intensity.

[0954] FIG. 58 represents the anti-tumor activity of AB-201 against the HER2-expressing cell lines HCC1954, SKOV-3, and NCI-N87. The data presented within the figure is a representative result from 3 separate tests. The red fluorescence integrated intensity or confluence of target cells for conditions without effector cells were normalized to 100%, and the fluorescence intensity and confluence of each co-culture condition were displayed for each time interval.

[0955] An enhanced anti-tumor activity of AB-201 against the HCC1954 cell line was observed at all observed time points compared to CBNK (E: T ratio=1:1, p value <0.0001, One-way ANOVA Kruskal-wallis test). As the fluorescence intensity of untreated HCC1954 cells at day 5 of co-culturing was set as 100%, it was confirmed that the anti-tumor activity of AB-201 against the HCC1954 cell line was high resulting in a reduced fluorescence of 25.3+1.0% (meanSEM) while the co-culture conditions with CBNK exhibited 82.9+6.1% (meanSEM) of fluorescence. In addition, it was confirmed that the anti-tumor activity of AB-201 occurred continuously during the co-culture process as the fluorescence intensity of HCC1954 cells continued to decrease during the 5 days. As the fluorescence intensity increased by approximately 6.6 fold over the 5 days for the HCC1954 cell only condition, it was demonstrated that the decrease in target cells were due to the cytotoxicity of AB-201.

[0956] An enhanced anti-tumor activity of AB-201 against the SKOV-3 cell line was observed at all time points compared to CBNK (E: T ratio=3:1, p value <0.0001, One-way ANOVA Kruskal-wallis test). As the fluorescence intensity of untreated SKOV3 cells at day 5 was set as 100%, it was shown that the co-culture condition with CBNK was at 95.72.5% (meanSEM) and the co-culture condition with AB-201 was 33.3+4.5% (mean=SEM), which indicates an enhanced anti-tumor activity of AB-201 in comparison to CBNK. In addition, while CBNK demonstrated a trend of increasing fluorescence intensity of the target cells as the anti-tumor activity gradually decreased following day 3 of co-culture, the anti-tumor activity of AB-201 was maintained continuously during all 5 days of co-culture. As the fluorescence intensity increased by approximately 5.9-fold over the 5 days for the SKOV-3 cell only condition, it was demonstrated that the decrease in target cells were due to the cytotoxicity of AB-201.

[0957] An enhanced anti-tumor activity of AB-201 against the NCI-N87 cell line was observed at all observed time points compared to CBNK cells (E:T ratio=1:1, p value <0.0001, One-way ANOVA Kruskal-wallis test). As the confluence of untreated NCI-N87 cells at day 5 of co-culturing was set as 100%, the measured confluence of CBNK and AB-201 cells resulted in 54.56.9% (meanSEM) and 38.8+4.2% (meanSEM), respectively. Hence, it was shown that the anti-tumor activity of AB-201 exceeded the high basal anti-tumor activity of CBNK cells against NCI-N87 cells. The upward pattern of the graph in the groups where NK cells have been added compared to the graph of untreated NCI-N87 at a 20-hour point can be explained by the increased confluence due to the influx of NK cells, and it was confirmed that confluence of the target cell decreased as the anti-tumor activity continued to occur during 5 days of co-culture. For NCI-N87 cell only condition, the confluence increased by approximately 3.8-foldover the 5 days, which confirmed that NCI-N87 cells have decreased due to the cytotoxicity of the NK cells.

Intracellular Cyotkine Measurement and Cell Surface CD107a Expression Measurement

[0958] After co-culturing NK cells and HER2-positive tumor cell lines (SKOV-3, HCC1954, NCI-N87) and a HER2-negative tumor cell line (K562) at an E:T ratio of 1:1 for 4 hours, respectively, the effector cytokines (IFN-, TNF-) and degranulation markers (CD107a) generated from NK cells were measured through flow cytometry. These results were obtained from a total of 3 repeat tests using CBNK and AB-201 generated from the same donor.

[0959] When CBNK or AB-201 was cultured alone without the target cell line, CD107a expressed in each cell was measured as 4.5=3.9% in CBNK and 2.8=0.7% in AB-201 (MeanSD). Under the co-culture condition with SKOV3. CD107a expression level in CBNK was 13.12.2% and in AB-201 61.2+10.1%, and when co-cultured with HCC1954, CD107a expression levels were 6.1=2.3% in CBNK and 38.115.1% in AB-201. Under the co-culture condition with NCI-N87, CBNK and AB-201 resulted in expression levels of 18.19.9% and 42.6+1.0%, respectively (FIG. 59, Table 17).

[0960] The intracellular expression of effector cytokine, IFN- was shown to be 4.8+6.3% and 1.40.6% for CBNK and AB-201 cells, respectively, in culture conditions without the target cell line. Conversely, IFN- expression was 13.47.5% and 51.9+2.9% for CBNK and AB-201, respectively, when co-cultured with SKOV-3. These data indicate a significant upregulation of IFN- expression in AB-201 compared to CBNK (** p<0.01, two-tailed t-test). When co-cultured with HCC1954, CBNK and AB-201 exhibited IFN- expression levels of 9.57.5% and 33.9+13.3%, respectively, which indicates a statistically significant difference (*p<0.05, two-tailed t-test). In the NCI-N87 co-culture, IFN- expression for CBNK and AB-201 were 18.19.9% and 42.6+1.0%, respectively. These data demonstrate a 2.3-fold higher IFN-Y expression for AB-201, however the results were not statistically significant (p=0.057, two-tailed t-test). The co-culture with K562 resulted in 61.4=9.9% in CBNK and 48.9-7.8% in AB-201 (FIG. 59, Table 17).

[0961] Through a similar method, the intracellular expression of TNF- was analyzed, and the protein was barely expressed with 1.71.3% in CBNK and 1.40.3% in AB-201 in culture conditions without the target cell line. Conversely, when co-cultured with SKOV3, the expression of TNF- increased to 17.0+5.5% with CBNK and 56.72.9% for CBNK and AB-201, respectively. Under the co-culture condition with HCC1954, TNF- expression was measured to be 5.42.0% and 22.3+6.9% for CBNK and AB-201, respectively. Both SKOV3 and HCC1954 co-cultures with AB-201 demonstrated significantly higher TNF- expression levels compared to co-cultures with CBNK (** p<0.01 and *p<0.05, two-tailed t-test for SKOV3 and HCC1954, respectively). Under the co-culture condition with NCI-N87, CBNK was 13.2+6.0%, and AB-201 was 32.0+11.4%, in which an approximately 2.4-fold higher expression was observed for AB-201 compared to CBNK. The co-culture with K562 resulted in 59.18.3% in CBNK and 45.55.3% in AB-201 (FIG. 59, Table 17).

[0962] Results indicate that AB-201 co-cultured with HER2-positive tumor cell lines expressed 10 to 20-fold higher levels of CD107a, 20 to 40-fold higher levels of IFN-, and 15 to 40-fold higher levels of TNF- compared to unstimulated NK cells (no target). When compared to CBNK, AB-201 co-cultured with HER2-positive tumor cell lines expressed a 4.5 to 6.3-fold higher expression of CD107a, 2.3 to 3.9-fold higher expression of IFN-, and 2.4 to 4.1-fold higher expression of TNF-.

TABLE-US-00017 TABLE 17 Summary of ICS data of CBNK, AB-201 against tumor cells (n = 3) CD107a IFN- TNF- Expression CBNK AB-201 CBNK AB-201 CBNK AB-201 (%) Mean SD Mean SD Mean SD Mean SD Mean SD Mean SD No target 4.5 3.9 2.8 0.7 4.8 6.3 1.4 0.6 1.7 1.3 1.4 0.3 K562 59.5 13.8 63.7 8.1 61.4 9.9 48.9 7.8 59.1 8.3 45.5 5.3 SKOV-3 13.1 2.2 61.2 10.1 13.4 7.5 51.9 2.9 17.0 5.5 56.7 4.7 HCC1954 6.1 2.3 38.1 15.2 9.5 7.5 33.9 13.3 5.4 2.0 22.3 6.9 NCI-N87 10.2 2.9 45.7 7.9 18.1 9.9 42.6 1.0 13.2 6.0 32.0 11.4

IFN- ELISA

[0963] The HER2-positive tumor cell lines (SKOV-3, HCC1954, NCI-N87) and a HER2-negative tumor cell line (K562) were co-cultured with CBNK or AB-201 at a ratio of 1:1 for 24 hours, respectively, and the concentration of IFN- was measured in the culture medium.

[0964] The data in FIG. 60 is an average of two experiments using the same batch of NK cells (run in triplicate), and exhibits the p values obtained through the T.TEST verification.

[0965] In co-culture with HER2-positive tumor cell lines and AB-201, the concentration of IFN- in the culture medium was approximately 5.3 to 7.8-fold higher than that of CBNK. In addition, HCC1954 and NCI-N87 showed a statistically significant difference. In co-culture with a HER2-negative tumor cell line (K562) and AB-201 the concentration of IFN- in culture medium was approximately 2.6-fold lower than that of CBNK.

[0966] IL-15 ELISA

[0967] The HER2-positive tumor cell lines (SKOV-3, HCC1954, NCI-N87) and a HER2-negative tumor cell line (K562) were co-cultured with CBNK or AB-201 cells at a ratio of 3:1 for 24 hours, respectively, and the concentration of IL-15 was measured in the culture medium. The data in FIG. 61 is an average of three experiments using the same batch of NK cells, and exhibits the p values obtained through the T.TEST verification.

[0968] In co-culture with tumor cells and AB-201, the concentration of IL-15 in the culture medium was approximately 3.5 to 6.4 fold higher than that of CBNK, and it demonstrated a statistically significant difference for all HER2+ tumor cell lines. In co-culture with AB-201 and a HER2-negative tumor cell line (K562), the concentration of IL-15 in culture medium was approximately 3.7-fold higher than that of CBNK, demonstrating a statistically significant difference.

CONCLUSIONS

[0969] The results for the short-term (4-hour) cytotoxicity analysis against HER2-positive tumor cells confirm significant anti-tumor activity of AB-201 compared to CBNK (ET ratio 10:1). (*p<0.05 for HCC1954 and NCI-N87, and ** p<0.01 for SKOV-3, compared with CBNK, two-tailed t-test). To confirm the direct anti-tumor activity of AB-201 against tumor cells, the long-term (5-day) cytotoxicity analysis was performed. The anti-tumor activity of AB-201 was higher in comparison to CBNK at all observed time points, and the anti-tumor activity of AB-201 persisted over the 5 days.

[0970] The activity of AB-201 against tumor cells was evaluated through cytokine secretion and CD107a expression measurement. Results indicate that AB-201 co-cultured with HER2-positive cell lines expressed 10 to20-fold higher levels of CD107a, 20 to40-fold higher levels of IFN-, and 15 to40-fold higher levels TNF- compared to its non-stimulated state (no target). In addition, a 4.5 to 6.3-fold higher expression of CD107a, a 2.3 to 3.9-fold higher expression of

[0971] IFN-, and a 2.4 to4.1-fold higher expression of TNF- was observed in comparison to CBNK cells. These results demonstrate HER2-dependent activity from AB-201.

[0972] IL-15 secretion which enhances the persistence of CAR-NK cells was analyzed following co-culture with the HER2-positive tumor cell lines. Results confirm that a 3.5 to 6.4 fold higher IL-15 level was secreted for AB-201 in comparison to CBNK.

[0973] In conclusion, this study confirmed specific and significant anti-tumor effects of AB-201 cells against HER2-positive tumor cells.

Example 16: AB-201 Therapy

Fixed Dosing

[0974] Patients with HER2+solid tumors are selected and treated with varying doses of AB-201 (110.sup.7 AB-201 cells per administration, 310.sup.7 AB-201 cells per administration, 1108 AB-201 cells per administration, 3108 AB-201 cells per administration or 1109 AB-201 cells per administration).

[0975] Some patients have advanced breast cancer (3rd line and beyond), some have gastric/GEJ cancer (2nd line and beyond, post trastuzumab).

Lymphodepleting Chemotherapy

[0976] Cyclophosphamide (500 mg/m.sup.2/day) and fludarabine (30 mg/m.sup.2/day) is administered IV daily for 3 consecutive days followed by two days of rest, starting 5 days before receiving AB-201 (i.e., from Day-5 through Day-3). Fludarabine and cyclophosphamide is administered by IV infusion, including renal dosing, as appropriate.

[0977] Each vial of AB-201 contains 11 mL of study drug at two different strengths: approximately 1.110.sup.7, 1.110.sup.8 or 1.110.sup.9 NK cells (e.g., 0.910.sup.9-1.310.sup.9. 0.910.sup.81.310.sup.8, or 0.910.sup.7-1.310.sup.7). No more than 10 mL (110.sup.7 or 110.sup.9 NK cells) is intended to be drawn from each vial. The prescribed dose of AB-201 is thawed and transferred aseptically into an IV bag for administration as an IV infusion, by gravity. When multiple vials are administered (i.e., Dose Levels 2 and 4), the vials needed for the dose are thawed simultaneously and transferred aseptically into a single IV bag for administration. AB-201 should be administered as soon as practical, preferably within 30 minutes and no longer than 90 minutes after thawing.

[0978] Dose levels for AB-201 are shown in Table 18.

TABLE-US-00018 TABLE 18 AB-201 Dose Levels Dose Level Dose 1 5 10.sup.6 cells 1 1 10.sup.7 cells 2 3 10.sup.7 cells 3 1 10.sup.8 cells 4 3 10.sup.8 cells 5 1 10.sup.9 cells

[0979] Cytokine support (IL-2) is not administered.

[0980] After the initial dose of AB-201, up to 3 additional doses are given (at days 29 and/or 57 or later, e.g., months 2. 3, and 5).

Weight-Based Dosing

[0981] Patients with HER2+solid tumors are selected and treated with varying doses of AB-201. The patients have one of the following: i) advanced/unresectable or metastatic breast cancer with HER2 overexpression (IHC2+) that is refractory to or intolerable of standard treatment, or for which no standard treatment is available: ii) advanced/unresectable or metastatic breast cancer with HER2 overexpression (IHC 3+ or IHC 2+/ISH+) that is relapsed to, refractory to, or intolerable of previous trastuzumab deruxtecan (T-DXd) based therapy: or advanced/unresectable or metastatic gastric or gastroesophageal junction adenocarcinoma with HER2 overexpression (IHC2+) that is refractory to or intolerable of standard treatment, or for which no standard treatment is available.

[0982] Cyclophosphamide (500 mg/m.sup.2/day) and fludarabine (30 mg/m.sup.2/day) is administered IV daily for 3 consecutive days followed by two days of rest, starting 5 days before receiving AB-201 (i.e., from Day-5 through Day-3). Fludarabine and cyclophosphamide is administered by IV infusion, including renal dosing, as appropriate.

[0983] The first dose of AB-201 is administered as a single IV infusion for approximately 5 to 10 minutes starting at 48 hours (but no more than 7 days) after completion of the lymphodepletion regimen. A 28-day observation period will then occur, at the end of which all relevant safety data will be reviewed. Patients may continue to receive second and third doses of AB201 every six weeks (in Week 7 and Week 13 (+1 week)), until unacceptable toxicity, progressive disease (PD), or withdrawal of consent. If the subject's weight changes by #10% of the baseline weight, then the AB-201 dose will be recalculated. Subsequent doses of AB-201 will be administered without prior lymphodepletion therapy.

[0984] Dose levels for AB-201 are shown in Table 19.

TABLE-US-00019 TABLE 19 AB-201 Dose Levels Dose Level Dose (per Body Weight) 1 1.5 10.sup.5 cells/kg 1 (Starting Dose) 4.0 10.sup.5 cells/kg 2 1.5 10.sup.6 cells/kg 3 4.0 10.sup.6 cells/kg 4 1.5 10.sup.7 cells/kg

TABLE-US-00020 SEQUENCES SEQIDNO:and DESCRIPTION SEQUENCE SEQIDNO:1 MEYASDASLDPEAPWPPAPRARACRVLPWALVAGLLLLLLLAAACAVFLACPWAVSGA Sequenceof4-1BBL RASPGSAASPRLREGPELSPDDPAGLLDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLA thatcanbeexpressed GVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAA byfeedercells GAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGA TVLGLFRVTPEIPAGLPSPRSE SEQIDNO:2 MALPVTALLLPLALLLHAARPQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVE Sequenceofa TNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCD membraneboundIL- SYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDSAKPTTTPAPRPPTPAPTIASQP 21(mbIL-21)thatcan LSLRPEACRPAAGGAVHTRGLDFACDIYIWAPLAGTCGVLLLSLVITLY beexpressedbyfeeder cells SEQIDNO:3 MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSLESESIVAGATTLFCLLHEGVIGP Sequenceofamutated QREEFPRDLSLISPLAQPVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANG TNFalpha(mTNF-a) VELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIK thatcanbeexpressed SPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIA byfeedercells L SEQIDNO:4 MERVQPLEENVGNAARPRFERNKLLLVASVIQGLGLLLCFTYICLHFSALQVSHRYPR SequenceofOX40L IQSIKVQFTEYKKEKGFILTSQKEDEIMKVQNNSVIINCDGFYLISLKGYFSQEVNIS thatcanbeexpressed LHYQKDEEPLFQLKKVRSVNSLMVASLTYKDKVYLNVTTDNTSLDDFHVNGGELILIH byfeedercells QNPGEFCVL SEQIDNO:5 RSKRSRLSHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRS CD28intracellular signalingdomain SEQIDNO:6 AGGAGTAAGAGGAGCAGGCTCCTGCACAGTGACTACATGAACATGACTCCCCGCCGCC CD28intracellular CCGGGCCCACCCGCAAGCATTACCAGCCCTATGCCCCACCACGCGACTTCGCAGCCTA signalingdomain TCGCTCC SEQIDNO:7 CGGAGCAAGAGGTCCCGCCTGCTGCACAGCGACTATATGAACATGACCCCACGGAGAC CodonOptimized CCGGCCCTACACGGAAACATTACCAGCCCTATGCTCCACCCCGGGACTTCGCAGCTTA CD28intracellular CAGAAGT signalingdomain SEQIDNO:8 ERVQPLEENVGNAARPRFERNK OX40Lintracellular signalingdomain SEQIDNO:9 LEENVGNAARPRFERNK OX40Lintracellular signalingdomain functionaldomain SEQIDNO:10 RPRFERNK OX40Lintracellular signalingdomain functionaldomain SEQIDNO:11 GAAAGGGTCCAACCCCTGGAAGAGAATGTGGGAAATGCAGCCAGGCCAAGATTCGAGA OX40Lintracellular GGAACAAG signalingdomain SEQIDNO:12 GAAAGAGTGCAGCCCCTGGAAGAGAATGTCGGGAATGCCGCTCGCCCAAGATTTGAAA Codonoptimized GGAACAAA OX40Lintracellular signalingdomain SEQIDNO:13 RVKESRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGL CD3signalingdomain YNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR SEQIDNO:14 AGAGTGAAGTTCAGCAGGAGCGCAGACGCCCCCGCGTACCAGCAGGGCCAGAACCAGC CD3signalingdomain TCTATAACGAGCTCAATCTAGGACGAAGAGAGGAGTACGATGTTTTGGACAAGAGACG TGGCCGGGACCCTGAGATGGGGGGAAAGCCGAGAAGGAAGAACCCTCAGGAAGGCCTG TACAATGAACTGCAGAAAGATAAGATGGCGGAGGCCTACAGTGAGATTGGGATGAAAG GCGAGCGCCGGAGGGGCAAGGGGCACGATGGCCTTTACCAGGGTCTCAGTACAGCCAC CAAGGACACCTACGACGCCCTTCACATGCAGGCCCTGCCCCCTCGC SEQIDNO:15 CGAGTGAAGTTCAGCAGGTCCGCCGACGCTCCTGCATACCAGCAGGGACAGAACCAGC CodonoptimizedCD3 TGTATAACGAGCTGAATCTGGGCCGGAGAGAGGAATACGACGTGCTGGACAAAAGGCG signalingodmian GGGCCGGGACCCCGAAATGGGAGGGAAGCCACGACGGAAAAACCCCCAGGAGGGCCTG TACAATGAGCTGCAAAAGGACAAAATGGCCGAGGCTTATTCTGAAATCGGGATGAAGG GAGAGAGAAGGCGCGGAAAAGGCCACGATGGCCTGTACCAGGGGCTGAGCACCGCTAC AAAGGACACCTATGATGCACTGCACATGCAGGCCCTGCCCCCTCGG SEQIDNO:16 GDVEXNPGP 2Acleavagemotif SEQIDNO:17 GSGEGRGSLLTCGDVEENPGP T2Acleavagesite SEQIDNO:18 GGCTCAGGTGAGGGGCGCGGGAGCCTGCTGACTTGTGGGGATGTAGAGGAAAATCCTG T2Acleavagesite GTCCT SEQIDNO:19 GSGATNFSLLKQAGDVEENPGP P2Acleavagesite SEQIDNO:20 GSGQCTNYALLKLAGDVESNPGP E2Acleavagesite SEQIDNO:21 GSGVKQTLNFDLLKLAGDVESNPGP F2Acleavagesite SEQIDNO:22 MRISKPHLRSISIQCYLCLLLNSHFLTEAGIHVFILGCFSAGLPKTEANWVNVISDLK IL-15 KIEDLIQSMHIDATLYTESDVHPSCKVTAMKCELLELQVISLESGDASIHDTVENLII LANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS- SEQIDNO:23 ATGAGAATCAGCAAACCACACCTCCGGAGCATATCAATCCAGTGTTACTTGTGCCTTC IL-15 TTTTGAACTCCCATTTCCTCACCGAGGCAGGCATTCATGTGTTCATATTGGGGTGCTT TAGTGCTGGGCTTCCGAAAACGGAAGCTAACTGGGTAAACGTCATCAGTGACCTTAAA AAAATTGAGGATCTTATCCAATCAATGCACATCGACGCGACTCTCTACACAGAATCTG ACGTACACCCGTCATGCAAAGTCACGGCAATGAAGTGTTTTCTTCTCGAGCTCCAAGT AATTTCCCTGGAGTCTGGCGATGCCTCCATCCACGATACGGTTGAAAATCTGATTATA TTGGCCAACAATAGCCTCAGTTCTAACGGTAACGTGACTGAAAGTGGCTGCAAAGAGT GCGAAGAGCTCGAAGAAAAGAATATCAAGGAGTTCCTCCAATCATTTGTTCACATTGT GCAAATGTTTATCAACACCTCTTGA SEQIDNO:24 ATGCGCATAAGTAAGCCTCATCTGCGGTCCATTTCTATACAATGTTATCTGTGCTTGC IL-15 TTTTGAACTCCCACTTTCTTACGGAAGCAGGCATTCATGTGTTCATTCTGGGTTGTTT TTCtGCCGGGCTGCCCAAAACCGAGGCCAACTGGGTCAACGTGATCAGCGACCTCAAG AAGATCGAGGATTTGATTCAAAGTATGCATATAGACGCCACACTCTATACTGAGTCCG ACGTTCACCCGAGTTGTAAAGTTACGGCTATGAAGTGCTTTTTGTTGGAACTCCAGGT GATTTCCCTTGAATCCGGCGATGCGAGCATCCACGATACGGTAGAGAATCTTATTATT CTGGCGAATAATTCTCTGTCTTCAAATGGGAATGTAACTGAGAGCGGTTGTAAAGAAT GCGAAGAACTTGAAGAAAAGAATATCAAGGAATTTCTTCAGAGTTTCGTGCATATTGT TCAAATGTTCATCAACACATCCTGA SEQIDNO:25 RSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRSERVQPLEENVGNAARPR CD28/OX40L/CD FERNKRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKN PQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQASPP R SEQIDNO:26 RSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPPRDFAAYRSERVQPLEENVGNAARPR CD28/OX40L/CD/ FERNKRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKN T2A/IL1-5 PQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPP RGSGEGRGSLLTCGDVEENPGPMRISKPHLRSISIQCYLCLLLNSHELTEAGIHVFIL GCFSAGLPKTEANWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCELLE LQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFV HIVQMFINTS- SEQIDNO:27 MALPVTALLLPLALLLHAARP CD8signalsequence SEQIDNO:28 ATGGCCTTACCAGTGACCGCCTTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCA CD8signalsequence GGCCG SEQIDNO:29 ATGGCACTTCCTGTTACAGCCCTCCTGCTCCCACTGGCTTTGCTGCTGCATGCTGCAC CodonOptimized GACCG CD8signalsequence SEQIDNO:30 DIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQQKPGKAPKTLIYRANRLVDGV anti-HER2scFv PSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFPWTFGQGTKVEIKGGGGSGGGG SGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTNYGVNWVRQAPGQGLEWMGWIN THTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARDDYYVRVDYWGQ GTTVTVSS SEQIDNO:31 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAGAGTGA anti-HER2scFv CCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAGCAGAA GCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACGGCGTG CCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAGCAGCC TGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCCTGGAC CTTCGGCCAGGGCACCAAGGTGGAGATCAAGGGTGGCGGTGGATCGGGCGGTGGTGGA TCTGGAGGAGGTGGCTCCCAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAAGAAGC CCGGCGCCAGCGTGAAGGTGAGCTGCAAGGCCAGCGGCTACACCTTCACCAACTACGG CGTGAACTGGGTGAGACAGGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGATCAAC ACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTTCAGCC TGGACACCAGCGTGAGCACCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAGGACAC CGCCGTGTACTACTGCGCCAGAGACGACTACTACGTGAGAGTGGACTACTGGGGCCAG GGCACCACCGTGACCGTGAGCAGC SEQIDNO:32 DIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQQKPGKAPKTLIYRANRLVDGV anti-HER2scFvVL PSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFPWTFGQGTKVEIK SEQIDNO:33 GACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAGAGTGA anti-HER2scFvVL CCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAGCAGAA GCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACGGCGTG CCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAGCAGCC TGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCCTGGAC CTTCGGCCAGGGCACCAAGGTGGAGATCAAG SEQIDNO:34 KASQDINSYLS anti-HER2scFv CDRL1 SEQIDNO:35 AAGGCCAGCCAGGACATCAACAGCTACCTGAGC anti-HER2scFv CDRL1 SEQIDNO:36 RANRLVD anti-HER2scFv CDRL2 SEQIDNO:37 AGAGCCAACAGACTGGTGGAC anti-HER2scFv CDRL2 SEQIDNO:38 LQYDEFPWT anti-HER2scFv CDRL3 SEQIDNO:39 CTGCAGTACGACGAGTTCCCCTGGACC anti-HER2scFv CDRL3 SEQIDNO:40 GGGGSGGGGSGGGGS anti-HER2scFvlinker SEQIDNO:41 GGTGGCGGTGGATCGGGCGGTGGTGGATCTGGAGGAGGTGGCTCC anti-HER2scFvlinker SEQIDNO:42 QVQLVQSGSELKKPGASVKVSCKASGYTFTNYGVNWVRQAPGQGLEWMGWINTHTGEP anti-HER2scFvVH TYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARDDYYVRVDYWGQGTTVTV SS SEQIDNO:43 CAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAAGAAGCCCGGCGCCAGCGTGAAGG anti-HER2scFvVH TGAGCTGCAAGGCCAGCGGCTACACCTTCACCAACTACGGCGTGAACTGGGTGAGACA GGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGATCAACACCCACACCGGCGAGCCC ACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTTCAGCCTGGACACCAGCGTGAGCA CCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAGGACACCGCCGTGTACTACTGCGC CAGAGACGACTACTACGTGAGAGTGGACTACTGGGGCCAGGGCACCACCGTGACCGTG AGCAGC SEQIDNO:44 NYGVN anti-HER2scFv CDRH1 SEQIDNO:45 AACTACGGCGTGAAC anti-HER2scFv CDRH1 SEQIDNO:46 WINTHTGEPTYAEEFKG anti-HER2scFv CDRH2 SEQIDNO:47 TGGATCAACACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGC anti-HER2scFv CDRH2 SEQIDNO:48 DDYYVRVDY anti-HER2scFv CDRH3 SEQIDNO:49 GACGACTACTACGTGAGAGTGGACTAC anti-HER2scFv CDRH3 SEQIDNO:50 AKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD CD8hinge SEQIDNO:51 GCGAAGCCCACCACGACGCCAGCGCCGCGACCACCAACACCGGCGCCCACCATCGCGT CD8hinge CGCAGCCCCTGTCCCTGCGCCCAGAGGCGTGCCGGCCAGCGGCGGGGGGCGCAGTGCA CACGAGGGGGCTGGACTTCGCCTGTGAT SEQIDNO:52 GCAAAACCTACCACAACTCCTGCACCACGCCCCCCTACTCCAGCACCTACCATCGCAT CodonOptimized CTCAGCCACTGAGTCTGCGACCAGAGGCCTGCCGGCCCGCCGCCGGGGGGCCGTCCA CD8hinge TACCAGAGGGCTGGACTTTGCCTGCGAT SEQIDNO:53 FWVLVVVGGVLACYSLLVTVAFIIFWV CD28transmembrane domain SEQIDNO:54 TTTTGGGTGCTGGTGGTGGTTGGTGGAGTCCTGGCTTGCTATAGCTTGCTAGTAACAG CD28transmembrane TGGCCTTTATTATTTTCTGGGTG domain SEQIDNO:55 TTTTGGGTCCTGGTGGTCGTGGGAGGGGTGCTGGCATGTTACTCACTGCTGGTCACCG CodonOptimized TGGCCTTCATCATCTTCTGGGTG CD28transmembrane domain SEQIDNO:56 MALPVTASLLPLALLLHAARPDIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQ HER2CAR QKPGKAPKTLIYRANRLVDGVPSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFP WTFGQGTKVEIKGGGGSGGGGSGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTN YGVNWVRQAPGQGLEWMGWINTHTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAE DTAVYYCARDDYYVRVDYWGQGTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEA CRPAAGGAVHTRGLDFACDFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDY MNMTPRRPGPTRKHYQPYAPPRDFAAYRSERVQPLEENVGNAARPRFERNKRVKESRS ADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKD KMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDASHMQALPPR SEQIDNO:57 ATGGCCTTACCAGTGACCGCCTTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCA HER2CAR GGCCGGACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAG AGTGACCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAG CAGAAGCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACG GCGTGCCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAG CAGCCTGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCC TGGACCTTCGGCCAGGGCACCAAGGTGGAGATCAAGGGTGGCGGTGGATCGGGCGGTG GTGGATCTGGAGGAGGTGGCTCCCAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAA GAAGCCCGGCGCCAGCGTGAAGGTGAGCTGCAAGGCCAGCGGCTACACCTTCACCAAC TACGGCGTGAACTGGGTGAGACAGGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGA TCAACACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTT CAGCCTGGACACCAGCGTGAGCACCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAG GACACCGCCGTGTACTACTGCGCCAGAGACGACTACTACGTGAGAGTGGACTACTGGG GCCAGGGCACCACCGTGACCGTGAGCAGCGCGAAGCCCACCACGACGCCAGCGCCGCG ACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGCCCAGAGGCG TGCCGGCCAGCGGCGGGGGGCGCAGTGCACACGAGGGGGCTGGACTTCGCCTGTGATT TTTGGGTGCTGGTGGTGGTTGGTGGAGTCCTGGCTTGCTATAGCTTGCTAGTAACAGT GGCCTTTATTATTTTCTGGGTGAGGAGTAAGAGGAGCAGGCTCCTGCACAGTGACTAC ATGAACATGACTCCCCGCCGCCCCGGGCCCACCCGCAAGCATTACCAGCCCTATGCCC CACCACGCGACTTCGCAGCCTATCGCTCCGAAAGGGTCCAACCCCTGGAAGAGAATGT GGGAAATGCAGCCAGGCCAAGATTCGAGAGGAACAAGAGAGTGAAGTTCAGCAGGAGC GCAGACGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAG GACGAAGAGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGG GGGAAAGCCGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGAT AAGATGGCGGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGG GGCACGATGGCCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCT TCACATGCAGGCCCTGCCCCCTCGC SEQIDNO:58 ATGGCACTTCCTGTTACAGCCCTCCTGCTCCCACTGGCTTTGCTGCTGCATGCTGCAC CodonOptimized GACCGGACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAG HER2CAR AGTGACCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAG CAGAAGCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACG GCGTGCCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAG CAGCCTGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCC TGGACCTTCGGCCAGGGCACCAAGGTGGAGATCAAGGGTGGCGGTGGATCGGGCGGTG GTGGATCTGGAGGAGGTGGCTCCCAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAA GAAGCCCGGCGCCAGCGTGAAGGTGAGCTGCAAGGCCAGCGGCTACACCTTCACCAAC TACGGCGTGAACTGGGTGAGACAGGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGA TCAACACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTT CAGCCTGGACACCAGCGTGAGCACCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAG GACACCGCCGTGTACTACTGCGCCAGAGACGACTACTACGTGAGAGTGGACTACTGGG GCCAGGGCACCACCGTGACCGTGAGCAGCGCAAAACCTACCACAACTCCTGCACCACG CCCCCCTACTCCAGCACCTACCATCGCATCTCAGCCACTGAGTCTGCGACCAGAGGCC TGCCGGCCCGCCGCCGGCGGGGCCGTCCATACCAGAGGGCTGGACTTTGCCTGCGATT TTTGGGTCCTGGTGGTCGTGGGAGGGGTGCTGGCATGTTACTCACTGCTGGTCACCGT GGCCTTCATCATCTTCTGGGTGCGGAGCAAGAGGTCCCGCCTGCTGCACAGCGACTAT ATGAACATGACCCCACGGAGACCCGGCCCTACACGGAAACATTACCAGCCCTATGCTC CACCCCGGGACTTCGCAGCTTACAGAAGTGAAAGAGTGCAGCCCCTGGAAGAGAATGT CGGGAATGCCGCTCGCCCAAGATTTGAAAGGAACAAACGAGTGAAGTTCAGCAGGTCC GCCGACGCTCCTGCATACCAGCAGGGACAGAACCAGCTGTATAACGAGCTGAATCTGG GCCGGAGAGAGGAATACGACGTGCTGGACAAAAGGCGGGGCCGGGACCCCGAAATGGG AGGGAAGCCACGACGGAAAAACCCCCAGGAGGGCCTGTACAATGAGCTGCAAAAGGAC AAAATGGCCGAGGCTTATTCTGAAATCGGGATGAAGGGAGAGAGAAGGCGCGGAAAAG GCCACGATGGCCTGTACCAGGGGCTGAGCACCGCTACAAAGGACACCTATGATGCACT GCACATGCAGGCCCTGCCCCCTCGG SEQIDNO:59 MALPVTALLLPLALLLHAARPDIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQ HER2CARwithT2A QKPGKAPKTLIYRANRLVDGVPSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFP andIL-15 WTFGQGTKVEIKGGGGSGGGGSGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTN YGVNWVRQAPGQGLEWMGWINTHTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAE DTAVYYCARDDYYVRVDYWGQGTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEA CRPAAGGAVHTRGLDFACDFWVLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDY MNMTPRRPGPTRKHYQPYAPPRDFAAYRSERVQPLEENVGNAARPRFERNKRVKESRS ADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKD KMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDASHMQALPPRGSGEGRGSALT CGDVEENPGPMRISKPHLRSISIQCYLCLLLNSHFLTEAGIHVFILGCFSAGLPKTEA NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDAS IHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS- SEQIDNO:60 ATGGCCTTACCAGTGACCGCCTTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCA HER2CARwithT2A GGCCGGACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAG andIL-15 AGTGACCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAG CAGAAGCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACG GCGTGCCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAG CAGCCTGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCC TGGACCTTCGGCCAGGGCACCAAGGTGGAGATCAAGGGTGGCGGTGGATCGGGCGGTG GTGGATCTGGAGGAGGTGGCTCCCAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAA GAAGCCCGGCGCCAGCGTGAAGGTGAGCTGCAAGGCCAGCGGCTACACCTTCACCAAC TACGGCGTGAACTGGGTGAGACAGGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGA TCAACACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTT CAGCCTGGACACCAGCGTGAGCACCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAG GACACCGCCGTGTACTACTGCGCCAGAGACGACTACTACGTGAGAGTGGACTACTGGG GCCAGGGCACCACCGTGACCGTGAGCAGCGCGAAGCCCACCACGACGCCAGCGCCGCG ACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGCCCAGAGGCG TGCCGGCCAGCGGCGGGGGGCGCAGTGCACACGAGGGGGCTGGACTTCGCCTGTGATT TTTGGGTGCTGGTGGTGGTTGGTGGAGTCCTGGCTTGCTATAGCTTGCTAGTAACAGT GGCCTTTATTATTTTCTGGGTGAGGAGTAAGAGGAGCAGGCTCCTGCACAGTGACTAC ATGAACATGACTCCCCGCCGCCCCGGGCCCACCCGCAAGCATTACCAGCCCTATGCCC CACCACGCGACTTCGCAGCCTATCGCTCCGAAAGGGTCCAACCCCTGGAAGAGAATGT GGGAAATGCAGCCAGGCCAAGATTCGAGAGGAACAAGAGAGTGAAGTTCAGCAGGAGC GCAGACGCCCCCGCGTACCAGCAGGGCCAGAACCAGCTCTATAACGAGCTCAATCTAG GACGAAGAGAGGAGTACGATGTTTTGGACAAGAGACGTGGCCGGGACCCTGAGATGGG GGGAAAGCCGAGAAGGAAGAACCCTCAGGAAGGCCTGTACAATGAACTGCAGAAAGAT AAGATGGCGGAGGCCTACAGTGAGATTGGGATGAAAGGCGAGCGCCGGAGGGGCAAGG GGCACGATGGCCTTTACCAGGGTCTCAGTACAGCCACCAAGGACACCTACGACGCCCT TCACATGCAGGCCCTGCCCCCTCGCGGCTCAGGTGAGGGGCGCGGGAGCCTGCTGACT TGTGGGGATGTAGAGGAAAATCCTGGTCCTATGAGAATTTCGAAACCACATTTGAGAA GTATTTCCATCCAGTGCTACTTGTGTTTACTTCTAAACAGTCATTTTCTAACTGAAGC TGGCATTCATGTCTTCATTTTGGGCTGTTTCAGTGCAGGGCTTCCTAAAACAGAAGCC AACTGGGTGAATGTAATAAGTGATTTGAAAAAAATTGAAGATCTTATTCAATCTATGC ATATTGATGCTACTTTATATACGGAAAGTGATGTTCACCCCAGTTGCAAAGTAACAGC AATGAAGTGCTTTCTCTTGGAGTTACAAGTTATTTCACTTGAGTCCGGAGATGCAAGT ATTCATGATACAGTAGAAAATCTGATCATCCTAGCAAACAACAGTTTGTCTTCTAATG GGAATGTAACAGAATCTGGATGCAAAGAATGTGAGGAACTGGAGGAAAAAAATATTAA AGAATTTTTGCAGAGTTTTGTACATATTGTCCAAATGTTCATCAACACTTCT SEQIDNO:61 ATGGCACTTCCTGTTACAGCCCTCCTGCTCCCACTGGCTTTGCTGCTGCATGCTGCAC CodonOptimized GACCGGACATCCAGATGACCCAGAGCCCCAGCAGCCTGAGCGCCAGCGTGGGCGACAG HER2CARwithT2A AGTGACCATCACCTGCAAGGCCAGCCAGGACATCAACAGCTACCTGAGCTGGTTCCAG andIL-15 CAGAAGCCCGGCAAGGCCCCCAAGACCCTGATCTACAGAGCCAACAGACTGGTGGACG GCGTGCCCAGCAGATTCAGCGGCAGCGGCAGCGGCCAGGACTACACCCTGACCATCAG CAGCCTGCAGCCCGAGGACTTCGCCACCTACTACTGCCTGCAGTACGACGAGTTCCCC TGGACCTTCGGCCAGGGCACCAAGGTGGAGATCAAGGGTGGCGGTGGATCGGGCGGTG GTGGATCTGGAGGAGGTGGCTCCCAGGTGCAGCTGGTGCAGAGCGGCAGCGAGCTGAA GAAGCCCGGCGCCAGCGTGAAGGTGAGCTGCAAGGCCAGCGGCTACACCTTCACCAAC TACGGCGTGAACTGGGTGAGACAGGCCCCCGGCCAGGGCCTGGAGTGGATGGGCTGGA TCAACACCCACACCGGCGAGCCCACCTACGCCGAGGAGTTCAAGGGCAGATTCGTGTT CAGCCTGGACACCAGCGTGAGCACCGCCTACCTGCAGATCAGCAGCCTGAAGGCCGAG GACACCGCCGTGTACTACTGCGCCAGAGACGACTACTACGTGAGAGTGGACTACTGGG GCCAGGGCACCACCGTGACCGTGAGCAGCGCAAAACCTACCACAACTCCTGCACCACG CCCCCCTACTCCAGCACCTACCATCGCATCTCAGCCACTGAGTCTGCGACCAGAGGCC TGCCGGCCCGCCGCCGGCGGGGCCGTCCATACCAGAGGGCTGGACTTTGCCTGCGATT TTTGGGTCCTGGTGGTCGTGGGAGGGGTGCTGGCATGTTACTCACTGCTGGTCACCGT GGCCTTCATCATCTTCTGGGTGCGGAGCAAGAGGTCCCGCCTGCTGCACAGCGACTAT ATGAACATGACCCCACGGAGACCCGGCCCTACACGGAAACATTACCAGCCCTATGCTC CACCCCGGGACTTCGCAGCTTACAGAAGTGAAAGAGTGCAGCCCCTGGAAGAGAATGT CGGGAATGCCGCTCGCCCAAGATTTGAWAGGAACAAACGAGTGAAGTTCAGCAGGTCC GCCGACGCTCCTGCATACCAGCAGGGACAGAACCAGCTGTATAACGAGCTGAATCTGG GCCGGAGAGAGGAATACGACGTGCTGGACAAAAGGCGGGGCCGGGACCCCGAAATGGG AGGGAAGCCACGACGGAAAAACCCCCAGGAGGGCCTGTACAATGAGCTGCAAAAGGAC AAAATGGCCGAGGCTTATTCTGAAATCGGGATGAAGGGAGAGAGAAGGCGCGGAAAAG GCCACGATGGCCTGTACCAGGGGCTGAGCACCGCTACAAAGGACACCTATGATGCACT GCACATGCAGGCCCTGCCCCCTCGGGGCTCAGGTGAGGGGCGCGGGAGCCTGCTGACT TGTGGGGATGTAGAGGAAAATCCTGGTCCTATGAGAATCAGCAAACCACACCTCCGGA GCATATCAATCCAGTGTTACTTGTGCCTTCTTTTGAACTCCCATTTCCTCACCGAGGC AGGCATTCATGTGTTCATATTGGGGTGCTTTAGTGCTGGGCTTCCGAAAACGGAAGCT AACTGGGTAAACGTCATCAGTGACCTTAAAAAAATTGAGGATCTTATCCAATCAATGC ACATCGACGCGACTCTCTACACAGAATCTGACGTACACCCGTCATGCAAAGTCACGGC AATGAAGTGTTTTCTTCTCGAGCTCCAAGTAATTTCCCTGGAGTCTGGCGATGCCTCC ATCCACGATACGGTTGAAAATCTGATTATATTGGCCAACAATAGCCTCAGTTCTAACG GTAACGTGACTGAAAGTGGCTGCAAAGAGTGCGAAGAGCTCGAAGAAAAGAATATCAA GGAGTTCCTCCAATCATTTGTTCACATTGTGCAAATGTTTATCAACACCTCT SEQIDNO:62 MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQG P04626ERBB2Human NLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAV Receptortyrosine- LDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFH proteinkinase(HER2) KNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLP TDCCHEQCAAGCTGPKHSDCLACLHENHSGICELHCPALVTYNTDTFESMPNPEGRYT FGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGME HLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEI TGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSG LALIHHNTHLCFVHTVPWDQLERNPHQALLHTANRPEDECVGEGLACHQLCARGHCWG PGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPE ADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKEPDEEGACQPCPINCTHSCVDLD DKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETEL VEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKV LRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRE NRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDEGLARLL DIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPA REIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRF VVIQNEDSGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVH HRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLP THDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAA RPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAESPAFD NLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV SEQIDNO:63 MKWVTFISLLFLESSAYSRGVERRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQC HumanAlbumin PFEDHVKSVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCA KQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFY APELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGE RAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICE NQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADEVESKDVCKNYAEAKD VFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVEDEEKPLVE EPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKH PEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYV PKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDEAAFVE KCCKADDKETCFAEEGKKLVAASQAALGL SEQIDNO:64 DIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQQKPGKAPKTLIYRANRLVDGV HER2CAR PSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFPWTFGQGTKVEIKGGGGSGGGG SGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTNYGVNWVRQAPGQGLEWMGWIN THTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARDDYYVRVDYWGQ GTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDEACDFW VLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPP RDFAAYRSERVQPLEENVGNAARPRFERNKRVKFSRSADAPAYQQGQNQLYNELNLGR REEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGH DGLYQGLSTATKDTYDALHMQALPPR SEQIDNO:65 DIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQQKPGKAPKTLIYRANRLVDGV HER2CARwithT2A PSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFPWTFGQGTKVEIKGGGGSGGGG andIL-15 SGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTNYGVNWVRQAPGQGLEWMGWIN THTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARDDYYVRVDYWGQ GTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDEACDFW VLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPP RDFAAYRSERVQPLEENVGNAARPRFERNKRVKESRSADAPAYQQGQNQLYNELNEGR REEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGH DGLYQGLSTATKDTYDALHMQALPPRGSGEGRGSLLTCGDVEENPGPMRISKPHLRSI SIQCYLCLLLNSHELTEAGIHVFILGCFSAGLPKTEANWVNVISDLKKIEDLIQSMHI DATLYTESDVHPSCKVTAMKCELLELQVISLESGDASIHDTVENLIILANNSLSSNGN VTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS- SEQIDNO:66 DIQMTQSPSSLSASVGDRVTITCKASQDINSYLSWFQQKPGKAPKTLIYRANRLVDGV HER2CARwithout PSRFSGSGSGQDYTLTISSLQPEDFATYYCLQYDEFPWTFGQGTKVEIKGGGGSGGGG OX40L SGGGGSQVQLVQSGSELKKPGASVKVSCKASGYTFTNYGVNWVRQAPGQGLEWMGWIN THTGEPTYAEEFKGRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARDDYYVRVDYWGQ GTTVTVSSAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDEACDEW VLVVVGGVLACYSLLVTVAFIIFWVRSKRSRLLHSDYMNMTPRRPGPTRKHYQPYAPP RDFAAYRSRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPR RKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQA LPPR

OTHER EMBODIMENTS

[0985] It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.