NANOFIBER SCAFFOLD AND MANUFACTURING DEVICE, MANUFACTURING METHOD AND APPLICATION THEREOF

Abstract

An anofiber scaffold manufacturing device, suitable for manufacturing a nanofiber scaffold. The nanofiber scaffold manufacturing device includes a feeder, a delivery tube, a spinning assembly, an automatic collection device, and an electrostatic generator. The feeder is suitable for storing a spinning raw material. The delivery tube has an input end and an output end. The input end is connected to the feeder. The spinning assembly includes a spinning electrode and a nozzle. The nozzle is connected to the spinning electrode, and the spinning assembly is connected to the output end of the delivery tube. The automatic collection device is disposed opposite to the spinning assembly. The electrostatic generator is connected to the spinning electrode and forming an electric field between the spinning electrode and the automatic collection device. A nanofiber scaffold manufacturing method, a nanofiber scaffold, and an application thereof are also provided.

Claims

1. A nanofiber scaffold manufacturing device, suitable for manufacturing a nanofiber scaffold, the nanofiber scaffold manufacturing device comprising: a feeder, suitable for storing a spinning raw material; at least one delivery tube, having an input end and an output end, wherein the input end is connected to the feeder; a spinning assembly, comprising a spinning electrode and at least one nozzle, wherein the at least one nozzle is connected to the spinning electrode, and the spinning assembly is connected to the output end of the delivery tube; an automatic collection device, disposed opposite to the spinning assembly; and an electrostatic generator, connected to the spinning electrode and forming an electric field between the spinning electrode and the automatic collection device.

2. The nanofiber scaffold manufacturing device according to claim 1, further comprising a movement control unit, wherein the movement control unit comprises a carrier seat, a first axis, and a first motor, the spinning assembly is disposed on the carrier seat, the carrier seat is movably connected to the first axis, and the first motor is suitable for driving the carrier seat to move along an axial direction of the first axis.

3. The nanofiber scaffold manufacturing device according to claim 2, wherein the movement control unit further comprises a second axis and a second motor, the axial direction of the first axis and an axial direction of the second axis are perpendicular to each other, the first axis is movably connected to the second axis, and the second motor is suitable for driving the first axis to move along the axial direction of the second axis.

4. The nanofiber scaffold manufacturing device according to claim 3, wherein the movement control unit further comprises a third axis and a third motor, the axial direction of the first axis, the axial direction of the second axis, and an axial direction of the third axis are perpendicular to each other, the second axis is movably connected to the third axis, and the third motor is suitable for driving the second axis to move along the axial direction of the third axis.

5. The nanofiber scaffold manufacturing device according to claim 1, further comprising a housing and a temperature-humidity control device, wherein the housing forms a spinning chamber, wherein the delivery tube, the spinning assembly, and the automatic collection device are disposed in the spinning chamber, and the temperature-humidity control device is suitable for detecting and controlling the temperature and humidity of the spinning chamber.

6. The nanofiber scaffold manufacturing device according to claim 5, wherein the temperature-humidity control device comprises a hot and cold air fan, and the hot and cold air fan is suitable for providing cold air or hot air to the spinning chamber to control the temperature and humidity of the spinning chamber.

7. The nanofiber scaffold manufacturing device according to claim 1, wherein the automatic collection device comprises a conveyor belt and at least two rollers, the conveyor belt is sleeved on the at least two rollers, and the at least two rollers rotate around their respective axes and drive the conveyor belt.

8. The nanofiber scaffold manufacturing device according to claim 7, wherein the conveyor belt is a metal track.

9. The nanofiber scaffold manufacturing device according to claim 1, further comprising a collection barrel, wherein the collection barrel is disposed on one side of the automatic collection device, and the collection barrel is suitable for collecting the nanofiber scaffold.

10. The nanofiber scaffold manufacturing device according to claim 1, wherein the at least one nozzle is a needleless nozzle.

11. The nanofiber scaffold manufacturing device according to claim 1, wherein the spinning electrode is a trough-type electrode.

12. A nanofiber scaffold manufacturing method, comprising: providing a nanofiber scaffold manufacturing device, comprising: a feeder, suitable for storing a spinning raw material; at least one delivery tube, having an input end and an output end, wherein the input end is connected to the feeder; a spinning assembly, comprising a spinning electrode and at least one nozzle, wherein the at least one nozzle is connected to the spinning electrode, and the spinning assembly is connected to the output end of the delivery tube; an automatic collection device, disposed opposite to the spinning assembly; and an electrostatic generator, connected to the spinning electrode and forming an electric field between the spinning electrode and the automatic collection device; using a polymer to prepare a polymer solution; adding at least one of an inorganic substance or a drug to the polymer solution to form a spinning raw material; controlling a temperature and humidity of the spinning chamber; storing the spinning raw material in the feeder, and transporting the spinning raw material to the spinning assembly through the delivery tube; turning on the electrostatic generator to form an electric field between the spinning electrode and the automatic collection device; and ejecting the spinning raw material by the at least one nozzle and receiving the ejected spinning raw material by the automatic collection device to form a nanofiber scaffold.

13. The nanofiber scaffold manufacturing method according to claim 12, wherein, in the spinning raw material, a concentration of the polymer is between 0.01 g/L and 100 g/L, and the polymer is selected from at least one of gelatin, collagen, hyaluronic acid, alginate, chitosan, polyethylene, polystyrene, styrene-butadiene rubber, styrene-isoprene rubber, polyester fiber, nylon fiber, epoxy resin, phenolic resin, and derivatives thereof.

14. The nanofiber scaffold manufacturing method according to claim 12, wherein, in the spinning raw material, a concentration of at least one of the inorganic substance or the drug is between 0.01 g/L and 100 g/L, and the inorganic substance is selected from at least one of calcium, phosphorus, sodium, potassium, magnesium, sulfur, chlorine, copper, iodine, manganese, zinc, iron, carbon oxides, carbonates, and cyanide, or the drug is selected from at least one of insulin, growth factors, metformin, and bisphosphate drugs.

15. The nanofiber scaffold manufacturing method according to claim 12, further comprising setting an injection flow rate of the at least one nozzle between 0.01 mL/s and 1 L/s.

16. The nanofiber scaffold manufacturing method according to claim 12, wherein the electric field is between 1 V/m and 1000 kV/m.

17. The nanofiber scaffold manufacturing method according to claim 12, wherein, after adding at least one of the inorganic substance or the drug to the polymer solution, the polymer solution is stirred continuously for 1 to 96 hours.

18. The nanofiber scaffold manufacturing method according to claim 12, wherein the temperature inside the spinning chamber is between 0 C. and 100 C.

19. A nanofiber scaffold manufactured by the nanofiber scaffold manufacturing method according to claim 1, wherein the nanofiber scaffold comprises a polymer and an inorganic substance or a drug, the polymer is selected from at least one of gelatin, collagen, hyaluronic acid, alginate, chitosan, polyethylene, polystyrene, styrene-butadiene rubber, styrene-isoprene rubber, polyester fiber, nylon fiber, epoxy resin, phenolic resin, and derivatives thereof, the inorganic substance is selected from at least one of calcium, phosphorus, sodium, potassium, magnesium, sulfur, chlorine, copper, iodine, manganese, zinc, iron, carbon oxides, carbonates, and cyanide, and the drug is selected from insulin, growth factors, metformin, and bisphosphate drugs.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention will become more readily apparent to those ordinarily skilled in the art after reviewing the following detailed description and accompanying drawings, in which:

[0026] FIG. 1 is a schematic diagram of a nanofiber scaffold manufacturing device according to an embodiment of the present invention;

[0027] FIG. 2 is a schematic diagram of a process of a nanofiber scaffold manufacturing method according to an embodiment of the present invention;

[0028] FIG. 3 is a schematic diagram of a process of a nanofiber scaffold manufacturing method according to another embodiment of the present invention;

[0029] FIG. 4 is a schematic diagram of an electron microscope image of the nanofiber scaffold manufactured according to the nanofiber scaffold manufacturing method according to an embodiment of the present invention; and

[0030] FIG. 5 is a schematic diagram of the radius distribution of the nanofibers in the nanofiber scaffold manufactured by the nanofiber scaffold manufacturing device according to an embodiment of the present invention.

DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS

[0031] The present invention will now be described more specifically with reference to the following embodiments. It is to be noted that the following descriptions of preferred embodiments of this invention are presented herein for purpose of illustration and description only. It is not intended to be exhaustive or to be limited to the precise form disclosed.

[0032] FIG. 1 is a schematic diagram of a nanofiber scaffold manufacturing device according to an embodiment of the present invention. FIG. 2 is a schematic diagram of a process of a nanofiber scaffold manufacturing method according to an embodiment of the present invention. FIG. 3 is a schematic diagram of a process of a nanofiber scaffold manufacturing method according to another embodiment of the present invention. FIG. 4 is a schematic diagram of an electron microscope image of the nanofiber scaffold manufactured according to the nanofiber scaffold manufacturing method according to an embodiment of the present invention. FIG. 5 is a schematic diagram of the radius distribution of the nanofibers in the nanofiber scaffold manufactured by the nanofiber scaffold manufacturing device according to an embodiment of the present invention. As shown in FIG. 1, the nanofiber scaffold manufacturing device 100 is designed for manufacturing a nanofiber scaffold 82. The nanofiber scaffold manufacturing device 100 includes a feeder 12, at least one delivery tube 121, a spinning assembly 14, an automatic collection device 22, and an electrostatic generator 30. The feeder 12 is used to store spinning raw materials (not shown, explained in subsequent paragraphs) for manufacturing the nanofiber scaffold 82. The delivery tube 121 has an input end and an output end, with the input end connected to the feeder 12 and the output end connected to the spinning assembly 14. The spinning assembly 14 includes a spinning electrode 141 and at least one nozzle 142, with the nozzle 142 connected to the spinning electrode 141. The automatic collection device 22 is disposed opposite the spinning assembly 14. When the nanofiber scaffold manufacturing device 100 operates, the nozzle 142 is designed to eject spinning raw materials toward the automatic collection device 22. The electrostatic generator 30 is connected to the spinning electrode 141 to create an electric field between the spinning electrode 141 and the automatic collection device 22.

[0033] Follow the above description. The feeder 12 transports the spinning raw material inside it to the spinning assembly 14 through the delivery tube 121 using mechanical means such as vibration, screw mechanism, peristalsis, compression, or belt movement. The automatic collection device 22 is connected to the ground wire 20. The electrostatic generator 30 provides charges to the spinning raw material being transported to the spinning assembly 14 via the spinning electrode 141. Utilizing the principle of attraction between positive and negative charges, the charged spinning raw material is ejected from the nozzle 142 toward the automatic collection device 22. The ejected spinning raw material is the nanofibers 80, which accumulate on the automatic collection device 22 to form the nanofiber scaffold 82.

[0034] In one embodiment, the spinning electrode 141 of the spinning assembly 14 may be a trough-type electrode containing a storage space. The number of delivery tubes 121 is one, while the number of nozzles 142 is multiple. The delivery tube 121 transports the spinning raw material to the storage space of the spinning electrode 141. The nozzles 142 are inserted in a single-row arrangement within the storage space of the spinning electrode 141 or arranged in an array within the storage space. The nozzles 142 are needleless and protrude from the spinning electrode 141. In one embodiment, the storage space of the spinning electrode 141 is equipped with a plurality of interconnected channels, with the opening end of each channel connected to a nozzle 142.

[0035] In one embodiment, the automatic collection device 22 includes a conveyor belt 221 and rollers 222. The number of rollers 222, for example, is two, but is not limited thereto. The conveyor belt 221 is sleeved on the two rollers 222, which rotate around their respective axes to drive the conveyor belt 221. As shown in FIG. 1, when the two rollers 222 rotate clockwise, the conveyor belt 221 sleeved on the two rollers 222 is driven, and the nanofiber scaffold 82 formed on the automatic collection device 22 is conveyed in a clockwise direction, for example, to the right, but not limited thereto. The conveyor belt 221 is made of metal material, such as a metal track, but is not limited thereto. In one embodiment, the nanofiber scaffold manufacturing device 100 further includes a collection barrel 70, which may be disposed on the right side of the automatic collection device 22, but is not limited thereto. The collection barrel 70 is suitable for collecting the nanofiber scaffold 82 transported by the automatic collection device 22. When the two rollers 222 rotate counterclockwise, the conveyor belt 221 sleeved on the two rollers 222 is driven, and the nanofiber scaffold 82 formed on the automatic collection device 22 is conveyed in a counterclockwise direction, for example, to the left, but not limited thereto. The collection barrel 70 may, for example, be disposed on the left side of the automatic collection device 22.

[0036] The nanofiber scaffold manufacturing device 100 further includes a movement control unit 40. The movement control unit 40 includes a carrier seat 410, a first axis 412, and a first motor 414. The spinning assembly 14 is disposed on the carrier seat 410. The axial direction of the first axis 412 is, for example, the X direction, but not limited thereto. The first motor 414 is designed to drive the carrier seat 410 to move along the axial direction of the first axis 412 (X direction). Additionally, the movement control unit 40 further includes a second axis 416 and a second motor 418. The axial direction of the second axis 416 is, for example, the Y direction, meaning that the axial direction of the first axis 412 and the axial direction of the second axis 416 are perpendicular to each other, but not limited thereto. The first axis 412 is movably connected to the second axis 416, and the second motor 418 is designed to drive the first axis 412 to move along the axial direction of the second axis 416 (Y direction).

[0037] Follow the above description. When the nanofiber scaffold manufacturing device 100 is activated, the spinning assembly 14 on the carrier seat 410 can achieve horizontal movement through the first axis 412 and the second axis 416 of the movement control unit 40. This movement facilitates the formation of a planar nanofiber scaffold 82 by the nanofibers 80 on the automatic collection device 22.

[0038] In one embodiment, the movement control unit 40 further includes a third axis 420 and a third motor 422. The axial direction of the third axis 420 is, for example, the Z direction, meaning that the axial directions of the first axis 412, the second axis 416, and the third axis 420 are mutually perpendicular, but not limited thereto. The second axis 416 is movably connected to the third axis 420, and the third motor 422 is designed to drive the second axis 416 to move along the axial direction of the third axis 420 (Z direction).

[0039] Follow the above description. When the nanofiber scaffold manufacturing device 100 is activated, the spinning assembly 14 on the carrier seat 410 can achieve single-plane or three-dimensional movement through the first axis 412, second axis 416, and third axis 420 of the movement control unit 40. This movement facilitates the formation of either a planar or a three-dimensional nanofiber scaffold 82 by the nanofibers 80 on the automatic collection device 22. In an unillustrated embodiment, there are multiple delivery tubes 121, which are suitable for stably transporting spinning raw material to the spinning assembly 14 from various directions during its movement.

[0040] Please refer to FIGS. 1, 4, and 5. The nanofiber scaffold manufacturing device 100 further includes a housing 50 and a temperature-humidity control device 60. The housing 50 forms a spinning chamber 52, in which the delivery tube 121, spinning assembly 14, and automatic collection device 22 are installed. The temperature-humidity control device 60 is designed to detect and regulate the temperature and humidity of the spinning chamber 52 to maintain the temperature and humidity for the spinning raw material inside the delivery tube 121 and spinning assembly 14. This ensures that the nanofibers 80 ejected from the nozzle 142 retain high quality, such as maintaining the quality of the radius or length of the nanofiber 80. In one embodiment, the temperature-humidity control device 60 includes a hot and cold air fan 62, which can provide hot or cold air to the spinning chamber 52 to regulate the temperature and humidity of the spinning chamber 52, but is not limited thereto. The temperature-humidity control device 60 further includes a heating lamp (not shown), which provides a stable heat source for the spinning chamber 52.

[0041] Please refer to FIGS. 1 and 2. A method for manufacturing a nanofiber scaffold according to an embodiment of the present invention describes includes the following steps: first, providing the nanofiber scaffold manufacturing device 100 (Step S2), which at least includes a feeder 12, a delivery tube 121, a spinning assembly 14, an automatic collection device 22, an electrostatic generator 30, a housing 50, and a temperature-humidity control device 60; next, preparing a polymer solution using a polymer (Step S4); next, adding at least one of inorganic substances or drugs to the polymer solution (Step S6) to form the spinning raw material; next, controlling the temperature and humidity of the spinning chamber 52, which is constructed within the housing 50 (Step S8), ensuring stable temperature and humidity for the spinning chamber 52; next, activating the electrostatic generator 30 (Step S10) to create an electric field between the spinning assembly 14 and the automatic collection device 22; and finally, storing the spinning raw material in the feeder 12 (Step S12), transporting the spinning raw material through the delivery tube 121 to the spinning assembly 14, ejecting the spinning raw material from the nozzle 142 toward the automatic collection device 22, receiving the spinning raw material to form the nanofiber scaffold 82. The spinning raw material carries charges provided by the electrostatic generator 30, utilizing the principle of attraction between opposite charges to direct the spinning raw material toward the automatic collection device 22.

[0042] The polymer solution uses sterile water as the solvent. While stirring, at least one of inorganic substances or drugs is added to form the spinning raw material. Based on the overall volume of the spinning raw material, the concentration of polymers in the spinning solution ranges between 0.01 g/L and 1000 g/L. Further, the polymer concentration can range between 0.1 g/L and 500 g/L, or between 1 g/L and 500 g/L, or between 50 g/L and 200 g/L. The polymers are selected from at least one of the following: gelatin, collagen, hyaluronic acid, alginic acid, chitosan, polyethylene, polystyrene, styrene-butadiene rubber, styrene-isoprene rubber, polyester fibers, nylon fibers, epoxy resin, phenolic resin, and their derivatives.

[0043] Based on the total volume of the spinning raw material, the concentration of either inorganic substances or drugs (at least one of them) ranges between 0.01 g/L and 1000 g/L. Furthermore, the concentration of inorganic substances or drugs can range between 0.01 g/L and 500 g/L, or between 0.01 g/L and 100 g/L, or between 0.01 g/L and 50 g/L. The inorganic substances are selected from at least one of the following: calcium, phosphorus, sodium, potassium, magnesium, sulfur, chlorine, copper, iodine, manganese, zinc, iron, carbon oxides, carbonates, and cyanides. The drugs are selected from at least one of the following: insulin, growth factors, metformin, and bisphosphonate drugs.

[0044] In one embodiment of this invention, the nanofiber scaffold manufactured by using the above-mentioned nanofiber scaffold manufacturing method includes polymers and either inorganic substances or drugs. The polymers are selected from at least one of the following: gelatin, collagen, hyaluronic acid, alginic acid, chitosan, polyethylene, polystyrene, styrene-butadiene rubber, styrene-isoprene rubber, polyester fibers, nylon fibers, epoxy resin, phenolic resin, and their derivatives. The inorganic substances are selected from at least one of the following: calcium, phosphorus, sodium, potassium, magnesium, sulfur, chlorine, copper, iodine, manganese, zinc, iron, carbon oxides, carbonates, and cyanides. The drugs are selected from at least one of the following: insulin, growth factors, metformin, and bisphosphonate drugs.

[0045] In one embodiment, for convenient collection of the nanofiber scaffold 82, the manufacturing method for the nanofiber scaffold 82 further includes installing a collection membrane (not shown) on the automatic collection device 22. The collection membrane is roll-mounted near the automatic collection device 22, with part of the collection membrane fixed onto the conveyor belt 221 of the automatic collection device 22. As the conveyor belt 221 rotates, the collection membrane advances continuously, allowing the nanofiber scaffold 82 to form at various positions on the collection membrane. The collection membrane then carries the nanofiber scaffold 82 to a designated collection point, such as a collection bucket 70, but this is not limited thereto. Once the production of the nanofiber scaffold 82 is completed, the nanofiber scaffold 82 is separated from the collection membrane.

[0046] The nanofiber scaffold manufacturing method further incudes establishing operating conditions. Please refer to FIG. 1. The operating conditions include but are not limited to: the rotation speed of the automatic collection device 22, the temperature of the spinning chamber 52, the humidity of the spinning chamber 52, the feeding speed of the feeder 12, the voltage supplied or the electric field formed by the electrostatic generator 30, the ejection flow rate of the nozzle 142, and the distance between the nozzle 142 and the automatic collection device 22. In one embodiment, the rotation speed of the automatic collection device 22 ranges between 1 cm/min and 200 cm/min, or between 3 cm/min and 100 cm/min, or between 3 cm/min and 30 cm/min. The temperature of the spinning chamber 52 ranges between 0 C. and 100 C., or between 35 C. and 60 C. The humidity of the spinning chamber 52 ranges between 20% and 80%. The feeding speed of the feeder 12 ranges between 1 mL/hour and 100 mL/hour, or between 1 mL/hour and 30 mL/hour. The voltage supplied by the electrostatic generator is set between 20 and 100 kV, or the electric field formed between the spinning electrode 141 in the spinning chamber 52 and the automatic collection device 22 ranges from 1 V/m to 1000 kV/m. The ejection flow rate of the nozzle 142 ranges from 0.01 mL/s to 1 L/s. The distance between the nozzle 142 and the automatic collection device 22 ranges from 3 cm to 20 cm.

[0047] In the nanofiber scaffold manufacturing method, after adding at least one of either inorganic substances or drugs to the polymer solution, continuous stirring is required for 5 minutes to 96 hours to ensure that the inorganic substances and/or drugs are fully and evenly dissolved. In one embodiment, based on the temperature and humidity of the spinning chamber 52, the stirring time is further adjusted to range between 10 minutes to 300 minutes or between 30 minutes to 240 minutes.

[0048] Please refer to FIG. 3. A nanofiber scaffold manufacturing method according to another embodiment includes the following steps: providing the nanofiber scaffold manufacturing device 100 (Step S100); dissolving 5-20 g of biocompatible polymer into 100 mL of sterile water while stirring, then adding 0.001 g to 10 g of a drug, heating to 40 C. to 80 C., and continuously stirring until the biocompatible polymer and the drug are fully dissolved to form the spinning raw material (Step S101); adjusting and controlling the temperature-humidity control device 60 (e.g., dehumidifier, heating lamp, hot and cold air fans), feeder 12, delivery tube 121, and electrodes of the electrostatic generator 30 in the nanofiber scaffold manufacturing device 100 (Step S102); installing a collection membrane on the automatic collection device 22 and setting the rotation speed of the automatic collection device 22 to 3 cm/min to 100 cm/min (Step S103); controlling the temperature of the spinning chamber 52 at 35 C. to 60 C. and the voltage of the electrostatic generator 30 at 20 kV to 100 kV (Step S104); and pouring the spinning raw material into the feeder 12 and ejecting the spinning raw material onto the collection membrane on the automatic collection device 22 at a rate of 1 mL/hour to 30 mL/hour through the nozzle 142 to form the nanofiber scaffold 82 (Step S105).

[0049] Follow the above description. The nanofiber scaffold manufacturing method further includes the following steps: once the feeder 12 has completely fed all the spinning raw materials, turning off the temperature-humidity control devices 60 (such as dehumidifiers, heating lamps, hot and cold air fans), the feeder 12, the automatic collection device 22, and the electrostatic generator 30, and removing the electrodes of the electrostatic generator 30, the delivery tube 121, and the feeder 12 (Step S106); detaching the collection membrane from the automatic collection device 22, and separating the nanofiber scaffold 82 from the collection membrane (Step S107); mixing the nanofiber scaffold 82 with 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form a cross-linked body, where the ratio of the nanofiber scaffold to EDC to NHS is 1:0.5-5:0.05-0.52, and preparing the absolute alcohol and ensuring that the nanofiber scaffold 82 is fully immersed in the cross-linked solution (Step S108); using the absolute alcohol to wash and removing the cross-linked solution three times, each lasting 1 to 4 hours (Step S109); after washing with pure water, pre-freezing at 80 C. (Step S110); and freeze-drying for 24 to 72 hours (Step S111), aiding in the subsequent formation of a carrier with the nanofiber scaffold 82 as its main body.

[0050] In one embodiment of present the invention, based on the structure of the nanofiber scaffold (horizontal or three-dimensional) and the drug components contained within the nanofiber scaffold, the scaffold can be utilized in the preparation of medical dressings, pharmaceuticals, compositions, or gels. Examples include epidermal dressings for wound repair, three-dimensional scaffolds or artificial bones for surgical, orthopedic, or dental applications, or pharmaceuticals, compositions, or gels that promote tissue regeneration, but the uses are not limited to these.

[0051] Based on the above, because the radius of the nanofibers in the nanofiber scaffold according to an embodiment of the present invention is sufficiently small (as shown in FIG. 5, approximately between 50 nanometers and 230 nanometers), it provides a larger contact area. The nanofiber scaffold produced through the aforementioned nanofiber scaffold manufacturing device and method can enhance the utilization efficiency and effectiveness of drugs.

[0052] While the invention has been described in terms of what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention needs not be limited to the disclosed embodiment. On the contrary, it is intended to cover various modifications and similar arrangements included within the spirit and scope of the appended claims which are to be accorded with the broadest interpretation so as to encompass all such modifications and similar structures.