SPROUT INHIBITOR CONTAINING DIHYDROCARVYL ACETATE AND USE THEREOF

Abstract

The present disclosure relates to a sprout inhibitor composition containing dihydrocarvyl acetate and use thereof. The sprout inhibitor composition contains the active ingredient dihydrocarvyl acetate or isomers thereof. In sprout inhibition tests for the sprout inhibitor in tobacco and potato, when the dosage of the active ingredient is equivalent to carvone, the sprout inhibitor exhibits a significantly better sprout inhibition effect than that of carvone. The sprout inhibitor provided by the present disclosure is effective on tobacco and potato plants in low dosage with long effective duration. Moreover, no phytotoxicity of the sprout inhibitor on the tobacco or the potato is found within the application range.

Claims

1. A sprout inhibitor composition, wherein an active ingredient of the sprout inhibitor is dihydrocarvyl acetate or isomers thereof.

2. The sprout inhibitor composition according to claim 1, wherein a content of the active ingredient of the dihydrocarvyl acetate or isomers thereof is 10-60% by weight.

3. The sprout inhibitor composition according to claim 2, wherein the sprout inhibitor is formulated into dosage forms for agricultural use.

4. The sprout inhibitor composition according to claim 3, wherein the dosage form of the sprout inhibitor composition is selected from the group consisting of an emulsifiable concentrate, an emulsion in water, a microemulsion, a soluble concentrate, and a hot fogging concentrate.

5. A method for inhibiting sprouting of tubers of potatoes and sweet potatoes by a sprout inhibitor, comprising fumigating the potatoes and sweet potatoes directly with the sprout inhibitor according to claim 1 with a hot fogging concentrate after piled storage and warehousing.

6. A method for inhibiting axillary buds of tobacco by applying a sprout inhibitor, comprising administrating the sprout inhibitor according to claim 1 by cup-drenching or smearing, and pouring the sprout inhibitor from different angles during the cup-drenching or the smearing; the axillary buds comprise axillary buds around topped wounds.

8. The method according to claim 6, wherein a content of the active ingredient of the dihydrocarvyl acetate or isomers thereof is 10-60% by weight.

9. The method according to claim 6, wherein the sprout inhibitor is formulated into any dosage form for agricultural use.

10. The method according to claim 6, wherein the dosage form of the sprout inhibitor is any selected from the group consisting of an emulsifiable concentrate, an emulsion in water, a microemulsion, a soluble concentrate, and a hot fogging concentrate.

11. The method according to claim 7, wherein a content of the active ingredient of the dihydrocarvyl acetate or isomers thereof is 10-60% by weight.

12. The method according to claim 7, wherein the sprout inhibitor is formulated into a dosage form for agricultural use.

13. The method according to claim 7, wherein the dosage form of the sprout inhibitor is one selected from the group consisting of an emulsifiable concentrate, an emulsion in water, a microemulsion, a soluble concentrate, and a hot fogging concentrate.

Description

DETAILED DESCRIPTION

[0024] In order to describe the objectives, technical solutions, and advantages of the present disclosure clearer, the present disclosure will be further described in detail below in conjunction with specific examples. It should be appreciated that the specific examples described herein are merely intended to explain the present disclosure, and are not intended to limit the present disclosure. Any modification, equivalent substitution and improvement made within the spirit and principle of the present disclosure shall be included within the claimed scope of the present disclosure.

[0025] The percentage of all formulations provided in the following examples is provided in percent of active by weight (converted into 100%). All processing technologies of formulations in compositions provided by the present disclosure are prior art, and the processing technologies can vary according to different conditions.

I. Preparation Examples

1. Example 1: 20% Dihydrocarvyl Acetate Microemulsion

[0026] Composition: 20% dihydrocarvyl acetate + 7% rapid penetrant T + 10% tristyrylphenol ethoxylates + deionized water to make up the balance.

[0027] The microemulsion was processed as follows: the above materials were fed into a mixing reactor to mix in a ratio of the formulation, and stirred to prepare the dihydrocarvyl acetate microemulsion.

2. Example 2: 20% Dihydrocarvyl Acetate Emulsion in Water

[0028] Composition: 20% dihydrocarvyl acetate + 4% Cremophor EL + 3% calcium alkylbenzene sulfonate + deionized water to make up the balance.

[0029] The emulsion in water was processed as follows: the above materials were fed into a mixing reactor to mix in a ratio of the formulation, stirred well, drawn into a shear mixing kettle, and shorn to prepare the dihydrocarvyl acetate emulsion in water.

3. Example 3: 50% Dihydrocarvyl Acetate Hot Fogging Concentrate

[0030] Composition: 50% dihydrocarvyl acetate + 3.5% fatty alcohol polyoxyethylene ether + 2.5% NP-10 + 4% attapulgite + 1% octanol + rapeseed oil to make up the balance.

[0031] The hot fogging concentrate was processed as follows: the above materials were fed into a mixing reactor to mix in a ratio of the formulation, and stirred to prepare the dihydrocarvyl acetate hot fogging concentrate.

4. Comparative Example 1: 50% Carvone Hot Fogging Concentrate

[0032] Composition: 50% carvone + 3.5% fatty alcohol polyoxyethylene ether + 2.5% NP-10 + 4% attapulgite + 1% octanol + rapeseed oil to make up the balance.

[0033] The hot fogging concentrate was processed as follows: the above materials were fed into a mixing reactor to mix in a ratio of the formulation, and stirred to prepare the carvone the hot fogging concentrate.

5. Comparative Example 2: 20% Carvone Emulsion in Water

[0034] Composition: 20% carvone + 4% Cremophor EL + 3% calcium alkylbenzene sulfonate + deionized water to make up the balance.

[0035] The emulsion in water was processed as follows: the above materials were fed into a mixing reactor to mix in a ratio of the formulation, stirred , drawn into a shear mixing kettle, and shorn to prepare the carvone emulsion in water.

II. Bioassay Example

1. Inhibitory Effects of Different Sprout Inhibitors on Axillary Buds of Tobacco

[0036] Test method: Fifty percent of tobacco plants were topped when the first central flowers bloomed, and sprout inhibitors were applied within 24 h after removal of 2 cm axillary buds and above. Using the cup-drenching method, the active ingredients with application doses as amounts in Examples 1 to 2 and Comparative Example 2 were diluted with water for application; each plant was applied with 20 mL sprout inhibitors, and clean water was sprayed as a control.

[0037] The area of each plot was no less than 40 m.sup.2 (1,100 plants/667 m.sup.2), and four rows of tobacco plants were planted in each plot. One guard row was arranged between plots. Five points were randomly selected, and five plants were point-surveyed at each sampling point. The number of axillary buds with a bud length of more than 2 cm was surveyed 15 and 30 days after application, respectively, and the sprout inhibition effect was calculated according to the following formula.

[00001]$\begin{array}{c}\text{Sprout}\text{inhibition}\text{rate}\left(\%\right)=\\ \left(\text{Number}\text{of}\text{control}\text{axillary}\text{buds}\text{-}\text{Number}\text{of}\text{treatment}\right)\\ \text{axillary}\left(\text{buds}\right)\text{/}\text{Number}\text{of}\text{control}\text{axillary}\text{buds}\text{*}\text{100}\end{array}$

[0038] The sprout inhibition effect in each treatment is shown in Table 1:

TABLE-US-00001 Sprout inhibition effects of different sprout inhibitors on tobacco Test compound Dosage of active ingredient (mL/plant) Sprout inhibition rate 15 days after application (%) Sprout inhibition rate 30 days after application (%) Example 1 0.5 98.3 81.7 0.1 88.4 70.3 Example 2 0.5 95.7 78.2 0.1 84.2 67.6 Comparative Example 2 0.5 88.2 66.4 0.1 81.9 55.3

[0039] From Table 1, when the dosage of the active ingredient in dihydrocarvyl acetate formulations in Examples 1 and 2 is 0.5 ml/plant, the control effect is better than that obtained by the carvone formulations at either 15 or 30 days after application. Particularly, the sprout inhibition rate of the dihydrocarvyl acetate formulation in Example 1 is still as high as 81.7% at 30 days after application, which is significantly better than that provided in Comparative Example 2.

2. Effects of Different Sprout Inhibitors on Storage Effect of Potato

[0040] Test method: Harvested potatoes were sorted, graded, ventilated and stored in a storehouse for two weeks; after the skin was dried, the potatoes were placed in a closed space and the active ingredients with application doses as amounts in Example 3 and Comparative Example 1 were atomized in a thermal fogger to make the hot fogging concentrate adsorb on the surface of each potato fully; after treatment, the potatoes were stored under natural storage conditions (at 20-25° C.) and results were observed.

[0041] Each storage box was considered as one treatment. The potatoes weighed not less than 50 kg for each treatment. In each treatment, 30 potatoes were selected, the total number of bud eyes with a bud length of more than 2 cm and the number of sprouted bud eyes were surveyed 15 and 30 days after application, and the sprout inhibition rate was calculated according to the following formula.

[00002]$\begin{array}{c}\text{Sprouting}\text{rate}\left(\%\right)=\\ \text{Number}\text{of}\text{sprouted}\text{bud}\text{eyes}\text{}/\text{}\text{Total}\text{number}\text{of}\text{surveyed}\text{bud}\text{eyes*}\\ 100;\end{array}$

[00003]$\begin{array}{l}\text{Sprout}\text{inhibition}\text{rate}\left(\%\right)=\left(\text{Control}\text{sprouting}\text{rate}-\text{Treatment}\text{sprouting}\text{rate}\right)/\text{Control}\\ \text{sprouting}\text{rate}\ast \text{100}\end{array}$

TABLE-US-00002 Sprout inhibition effects of different sprout inhibitors on potatoes Test Compound Dosage of active ingredient (mL/kg) Sprout inhibition rate 15 days after application (%) Sprout inhibition rate 30 days after application (%) Example 3 0.2 100 99.5 0.025 90.2 75.8 Comparative Example 1 0.2 84.3 73.6 0.025 66.7 56.1

[0042] From Table 2, compared with the carvone hot fogging concentrate, different concentrations of the dihydrocarvyl acetate hot fogging concentrate in Example 3 have better sprout inhibitor on potatoes 15 and 30 days after inhibition.