Device for nucleic acid amplification reaction

Abstract

This device comprises: a casing with an upper surface including a sample-dripping portion receiving a liquid sample containing nucleic acid and being dripped from a nozzle; a reaction tube: outwardly projecting from an end of the casing; including a storage space therein; and being formed so as to be installed within a measurement apparatus; a filter carrying the nucleic acid contained in the liquid sample; a filter-supporting body stored within the casing to support the filter in a manner such that the filter is capable of taking: a contacting position wherein the filter contacts with the liquid sample right below the sample-dripping portion; and a reaction position wherein the filter is positioned within the storage space of the reaction tube; and absorbing material capable of taking: a press-attaching position wherein the absorbing material is press-attached to the filter in the contacting position so that the filter absorbs the liquid sample contacting therewith; and a separating position wherein the absorbing material is removed from the press-attaching position so that the filter is allowed to freely move.

Claims

1. A device for nucleic acid amplification reaction, comprising: a casing with an upper surface including a sample-dripping portion capable of receiving a liquid sample dripped from a nozzle and containing nucleic acid; a reaction tube: outwardly projecting from an end of the casing; including a storage space therein; and being formed so as to be installed within a measurement apparatus; a filter carrying thereon the nucleic acid contained in the liquid sample; a filter-supporting body stored within the casing to support the filter in a manner such that the filter is capable of taking: a contacting position wherein the filter contacts with the liquid sample right below the sample-dripping portion; and a reaction position wherein the filter is positioned within the storage space of the reaction tube; and an absorbing material capable of taking: a press-attaching position wherein the absorbing material is press-attached to the filter in the contacting position so that the filter absorbs the liquid sample contacting therewith; and a separating position wherein the absorbing material is removed from the press-attaching position so that the filter is allowed to freely move, wherein the filter-supporting body falls downward within the casing, thereby the absorbing material moves from the press-attaching position to the separating position.

2. The device for nucleic acid amplification reaction as defined in claim 1, further comprising: a cleaning liquid pot carrying cleaning fluid therein; and a breaking projection allowing the cleaning fluid within the cleaning liquid pot to flow toward the sample-dripping portion.

3. The device for nucleic acid amplification reaction as defined in claim 1, wherein a pressing part drives the filter-supporting body to be supported so that the filter-supporting body is allowed to move from the contacting position to the reaction position.

4. The device for nucleic acid amplification reaction as defined in claim 1, wherein the reaction tube is composed of a transparent material.

5. The device for nucleic acid amplification reaction as defined in claim 1, wherein the reaction tube holds at least a part of reagent required for the nucleic acid amplification reaction.

6. The device for nucleic acid amplification reaction as defined in claim 5, wherein the reagent required for the nucleic acid amplification reaction contains hot-melt polymeric compound that is solidified and/or gelled before the nucleic acid amplification reaction, and that is heated to become liquid in the nucleic acid amplification reaction, the reagent being held within the reaction tube.

7. The device for nucleic acid amplification reaction as defined in claim 1, wherein the absorbing material is composed of a water absorptive soft material selecting from the group consisting of filter paper; a polymeric absorbing material; and glass fiber filter paper.

8. The device for nucleic acid amplification reaction as defined in claim 1, wherein a silica particle is fixed to the filter.

9. The device for nucleic acid amplification reaction as defined in claim 1, wherein the filter is composed of polymeric membrane.

10. The device for nucleic acid amplification reaction as defined in claim 9, wherein the filter is composed of hydrophilic PTFE (polytetrafluoroethylene).

11. The device for nucleic acid amplification reaction as defined in claim 1, wherein the filter-supporting body holds reagent required for the nucleic acid amplification reaction.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) FIG. 1 is a perspective diagram of a device for nucleic acid amplification reaction when dripping a sample in an embodiment of the present invention;

(2) FIG. 2 is a perspective diagram of the measurement apparatus in the embodiment of the present invention;

(3) FIG. 3 is a ground plan showing the principle of a measurement apparatus in the embodiment of the present invention;

(4) FIG. 4 is an exploded perspective view of the device for nucleic acid amplification reaction in the embodiment of the present invention;

(5) FIG. 5(a) is a perspective diagram of a filter-supporting body in the embodiment of the present invention;

(6) FIG. 5(b) is a perspective diagram of a cleaning liquid pot in the embodiment of the present invention;

(7) FIG. 5(c) is a perspective diagram of a cover for cleaning liquid pot in the embodiment of the present invention;

(8) FIG. 6(a) is a first explanatory diagram of a first pressing element in the embodiment of the present invention;

(9) FIG. 6(b) is a second explanatory diagram of the first pressing element in the embodiment of the present invention;

(10) FIG. 6(c) is a third explanatory diagram of the first pressing element in the embodiment of the present invention;

(11) FIG. 7(a) is a first explanatory diagram of the cleaning liquid pot in the embodiment of the present invention of operation;

(12) FIG. 7(b) is a second explanatory diagram of the cleaning liquid pot in the embodiment of the present invention of operation;

(13) FIG. 7(c) is a third explanatory diagram of the cleaning liquid pot in the embodiment of the present invention of operation;

(14) FIG. 8(a) is a perspective diagram of the filter-supporting body and absorbing material in the embodiment of the present invention;

(15) FIG. 8(b) is a perspective diagram of a lower casing and a support plate in the embodiment of the present invention;

(16) FIG. 9(a) is a first explanatory diagram of a support in the embodiment of the present invention;

(17) FIG. 9(b) is a second explanatory diagram of the support in the embodiment of the present invention;

(18) FIG. 10(a) is a first explanatory diagram of the absorbing material in the embodiment of the present invention;

(19) FIG. 10(b) is a second explanatory diagram of the absorbing material in the embodiment of the present invention;

(20) FIG. 10(c) is a third explanatory diagram of the absorbing material in the embodiment of the present invention;

(21) FIG. 10(d) is a fourth explanatory diagram of the absorbing material in the embodiment of the present invention;

(22) FIG. 10(e) is a fifth explanatory diagram of the absorbing material in the embodiment of the present invention;

(23) FIG. 10(f) is a sixth explanatory diagram of the absorbing material in the embodiment of the present invention;

(24) FIG. 11(a) is a first operation explanatory diagram of the filter-supporting body in the embodiment of the present invention;

(25) FIG. 11(b) is a second operation explanatory diagram of the filter-supporting body in the embodiment of the present invention;

(26) FIG. 11(c) is a first operation explanatory diagram of the filter-supporting body in the embodiment of the present invention; and

(27) FIG. 12 is a graph showing a measurement result with respect to a positive control and a negative control in the embodiment of the present invention.

BRIEF DESCRIPTION OF SYMBOLS

(28) 1: First Pressing Element 2: Second Pressing Element 3: Third Pressing Element 10: Device for Nucleic Acid Amplification Reaction 100: Upper Casing 101: Sample-dripping Portion 101a: Discharge port 101b: Chute 102: Reaction Device-holding Portion 103: Stopper 110: Cover for Cleaning Liquid Pot 111: Breaking Projection 120: Cleaning Liquid Pot 121: Injecting Port 122: Projection 200: Reaction Tube 201: Reagent 300: Filter-supporting Body 301: Filter 302: Reagent Application Part 303: Driving Rib 304: “O” Ring 400: Absorbing Material Installation Plate 401: Absorbing Material 500: Support Plate 501: Support 502: Lock Member 503: Pressing Part 600: Lower Casing 601: Stopper 602: Opening 700: Measurement Apparatus 701: Insertion Port 702: Monitor Screen 703: Temperature Control Block 704: Fluorescence Measurement Unit 704a: Illuminator 704b: Light-Receiving Sensor 705: Door 800: Dripping Nozzle 801: Extraction Container 802: Hand