MANNANASE FOR FORMULATIONS HAVING PH 5-12

Abstract

Method to remove mannan comprising stains by contacting at least one mannan comprising stain with a mannanase at least 80% identical to SEQ ID NO: 1.

Claims

1. Method of removing mannan comprising stains at temperatures in the range of about 5-60° C. by the steps of contacting at least one mannan comprising stain with a mannanase at least 80% identical to SEQ ID NO: 1.

2. Method according to claim 1, wherein the mannan-comprising stain is comprised on a textile.

3. A liquid enzyme preparation comprising a mannanase at least 80% identical to SEQ ID NO: 1, at least one compound stabilizing the liquid enzyme preparation as such, at least one solvent, and optionally at least one enzyme stabilizer.

4. The liquid enzyme preparation according to claim 2, wherein the enzyme preparation has a pH in the range of 5-12.

5. A laundering detergent formulation with increased washing or cleaning performance towards mannan-comprising stains, wherein the formulation comprises a mannanase at least 80% identical to SEQ ID NO: 1, and wherein the formulation has a pH in the range of 5-12.

6. The formulation according to claim 5, wherein the formulation comprises at least one component selected from surfactants, builders, and hydrotropes in amounts effective in maintaining the physical characteristics of the formulation and/or in amounts effective in cleaning.

7. A method to provide a liquid laundering detergent effective towards mannan comprising stains, comprising the steps of mixing in one or more steps (a) at least one mannanase at least 80% identical to SEQ ID NO: 1, and (b) at least one detergent component selected from surfactants, builders, and hydrotropes present in amounts effective in cleaning and/or in amounts effective in maintaining the physical characteristics of the detergent.

8. A method of washing or cleaning, comprising the steps of (a) providing at least one mannan-comprising stain; (b) providing a formulation according to claim 5 (c) contacting the mannan-comprising stain (a) with the formulation of (b).

9. The method according to claim 8, wherein the mannan-comprising stain is provided on a textile (a); and wherein the polypeptide comprised in the detergent (b) removes the mannan-comprising stain from the textile (a).

10. The method according to claim 9, wherein the mannan-comprising stain comprises at least one galactomannan.

11. (canceled)

12. The method of claim 1, wherein the mannan comprising stain comprises at least one galactomannan.

13. The laundering detergent formulation of claim 5 with increased washing or cleaning performance towards mannan-comprising stains comprising locust bean gum and/or guar gum.

14. The laundering detergent formulation of claim 5, wherein the formulation is liquid.

15. The method of washing or cleaning of claim 8, wherein the mannan-comprising stain is contacted with the formulation of (b) at a washing or cleaning temperature in the range of 5-60° C.

16. The method according to claim 10, wherein the mannan-comprising stain comprises locust bean gum and/or guar gum.

Description

EXAMPLE 1

Evaluation of Mannanases in Rotawash

[0171] The following mannanase enzymes Man01, Man02, and Man03 were tested in liquid detergent ES1_C (pH 8) and Persil non-Bio (pH 7.2) at 40° C. or 25° C. washing temperature:

[0172] Man01: mannanase according to SEQ ID NO:1

[0173] Man02: Mannanase according to SEQ ID NO:2

[0174] Man03: Mannanase according to SEQ ID NO:3

TABLE-US-00001 ES1_C components Conc. wt % Maranil DBS/LC linear alkylbenzene 5.5 sulfonate, anionic surfactant Edenor coco fatty C.sub.12-C.sub.18 2.4 acid coco fatty acid Lutensol AO7 alkyl polyethyleneglycol 5.5 ether, non-ionic surfactant Texapon N70 sodium laureth 5.5 sulfate 2EO, anionic surfactant 1,2 propylene 6.0 glycol C.sub.2H.sub.5OH 2.0 KOH 2.2 Citrate 3.0 Mannanase according to Tables Ex1a and Ex1b pH 8-8.5 H.sub.2O up to 100%

[0175] CFT C-S-43/guar gum stain monitors or CFT C-S-73/locust bean gum stain monitors (CFT, Vlaardingen, NL) were washed together with cotton ballast fabric and steel balls in wash liquor using base formulation ES1_C in the Rotawash (Rotawash M228, SDL Atlas Inc., USA) under the following washing conditions:

TABLE-US-00002 Washing liquor 500 ml # Steel balls 20 Washing time/temperature 40 min at 25° C. or 40° C. Dosage Mannanase From 0 to 0.1 ppm Dosage detergent 1 g/L ES1_C or 3.5 g/L Persil non-Bio Washing cycles  1 Water hardness 2.7 mmol/L Ballast fabric 4 g cotton fabric 283 Soiled swatches 2 × 0.4 g CS-43 or 2 × 0.4 g C-S-73

[0176] After the washing, the fabrics were rinsed and dried in the air. The wash performance for the single stains was determined by measuring the average color intensity of the soiled fabric after wash with a flatbed color image scanner from EPSON (Expression 11000XL). In general, the higher the average intensity value, the better the performance. The results are also outlined below in Tables Ex1a and Ex1b and Ex1c.

TABLE-US-00003 TABLE Ex1a Man01 wash performance at 40° C. on guar gum stain (C-S-43) in ES1_C detergent formulation; values in average intensity (RGB) Dose values in average intensity (RGB) ppm SEQ ID NO: 2 Man01 SEQ ID NO: 3 0 0.64 0.64 0.64 0.01 0.67 0.68 0.02 0.68 0.68 0.67 0.05 0.68 0.70 0.1 0.68 0.72 0.69

TABLE-US-00004 TABLE Ex1b Man01 wash performance at 40° C. on locust bean gum stain (C-S-73) in ES1_C detergent formulation; values in average intensity (RGB) Dose values in average intensity (RGB) ppm SEQ ID NO: 2 Man01 SEQ ID NO: 3 0 0.70 0.70 0.70 0.05 0.78 0.78 0.1 0.76 0.78 0.80

TABLE-US-00005 TABLE Ex1c Man01 wash performance at 40° C. on guar gum stain (C- S-43) in Persil non-Bio detergent; values average intensity (RGB) Dose values in average intensity (RGB) ppm SEQ ID NO: 2 Man01 SEQ ID NO: 3 0 0.57 0.57 0.57 0.01 0.60 0.64 0.60 0.05 0.62 0.66 0.64 0.1 0.64 0.66 0.65

TABLE-US-00006 TABLE Ex1d Man01 wash performance at 40° C. on locust bean gum stain (C-S-73) in Persil non-Bio detergent; values average intensity (RGB) Dose values in average intensity (RGB) ppm SEQ ID NO: 2 Man01 SEQ ID NO: 3 0 0.64 0.64 0.64 0.01 0.74 0.74 0.70 0.05 0.73 0.73 0.74 0.1 0.73 0.73 0.74

TABLE-US-00007 TABLE Ex1e Man01 wash performance at 25° C. on guar gum stain (C-S- 43) in Persil non-Bio detergent; values in average intensity (RGB) Dose values in average intensity (RGB) ppm SEQ ID NO: 2 Man01 SEQ ID NO: 3 0 0.58 0.58 0.58 0.01 0.60 0.64 0.02 0.61 0.64 0.61 0.05 0.62 0.67 0.1 0.63 0.68 0.65