USE OF TAURINE IN PREVENTION AND/OR TREATMENT OF DISEASES INDUCED BY VIRUSES OF GENUS CORONAVIRUS AND/OR GENUS ROTAVIRUS

Abstract

The present invention provides use of taurine in prevention and/or treatment of diseases induced by viruses of genus coronavirus and/or genus rotavirus, for example, porcine epidemic diarrhea, porcine transmissible gastroenteritis, rotavirus diarrhea and the like.

Claims

1. (canceled)

2. A method for preventing or treating porcine viral diarrhea by providing a patient with an effective dose of taurine.

3. The method according to claim 2, wherein the porcine viral diarrhea is a disease induced by viruses of genus coronavirus or genus rotavirus.

4. The method according to claim 3, wherein the disease induced by viruses of the genus coronavirus is porcine epidemic diarrhea or porcine transmissible gastroenteritis.

5. (canceled)

6. (canceled)

7. (canceled)

8. A drug for prevention and/or treatment of porcine viral diarrhea, comprising an effective dose of taurine.

9. (canceled)

10. The method according to claim 2, wherein an effective dose of taurine is 10-20000 mg per kilogram of body weight.

11. The drug according to claim 8, wherein the porcine viral diarrhea is a disease induced by viruses of genus coronavirus or genus rotavirus.

12. The drug according to claim 11, wherein the disease induced by viruses of the genus coronavirus is porcine epidemic diarrhea.

13. The method according to claim 4, wherein an effective dose of taurine is 10-20000 mg per kilogram of body weight.

14. The method according to claim 3, wherein an effective dose of taurine is 10-20000 mg per kilogram of body weight.

Description

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT

[0022] The present invention will be further described in detail by specific embodiments. Unless otherwise specified, experiment methods hereinafter are all conventional methods in the art. Unless otherwise specified, the used ingredients or materials are all ingredients or materials that are commercially available. Content described below is preferred implementation of the present invention. It should be noted that for those ordinarily skilled in the art, some improvements may be made without departing from the principles of the present invention and such improvements shall be deemed as falling within the protection scope of the present invention.

Embodiment 1 Use of Taurine in Prevention of Porcine Epidemic Diarrhea of Newborn Piglets

[0023] Prevention of the porcine epidemic diarrhea on the newborn piglets with taurine was implemented by orally administering taurine to pregnant sows.

[0024] Drug administration time: 3-30 days before birth of piglets until 15 days after birth of piglets. In consideration of a use-cost, the drug administration time is preferably 5-20 days before birth of piglets until 10 days after birth of piglets, and more preferably 7-14 days before birth of piglets until 5 days after birth of piglets. The effect is subject to an obvious time-effect relationship. The drug administration time for before birth of piglets directly determines a disease incidence of the newborn piglets.

[0025] Drug administration dosage: each pregnant sow was administered with 5-500 g of taurine per day, and the administration was in a single dose or in divided doses. The effect is subject to an obvious quantity-effect relationship.

[0026] Specific operations: pregnant sows were selected and excrement thereof was tested by RT-PCR to determine the pregnant sows carrying PEDV in vivo. 60 PEDV-carried pregnant sows were divided into two groups randomly. Control group: conventional feeding; experimental group: conventional feeding+50 g per sow per day of taurine. Prevention results of the two groups are listed in Table 1. As seen from Table 1, the disease incidence and a death rate of the experimental group which was administered with taurine are obviously decreased compared to those of the control group (P<0.01).

TABLE-US-00001 TABLE 1 Disease Death rate incidence of of the Dosage the new born newborn Group of taurine piglets (%) piglets (%) Control group 0 100% 96.6% Experimental 50 g per  85% 75.1% group sow per day

Embodiment 2 Use of Taurine in Treatment of the Porcine Epidemic Diarrhea of Newborn Piglets

[0027] Treatment of the porcine epidemic diarrhea on newborn piglets with taurine was implemented by orally administering a taurine solution to diseased suckling piglets and administering taurine to lactating sows, that is, simultaneous treatment of both the suckling piglets and the lactating sows.

[0028] Drug administration time: the administration by drenching/mixed-feeding was carried out on the day when the disease occurred in the suckling piglets and a non-interrupted administration lasted for 3-10 days. Lactating sows: non-interrupted oral administration lasted for 5-10 days.

[0029] Drug administration dosage: the administration by drenching and/or mixed-feeding was carried out on the day when the disease occurred in the suckling piglets. The administration by drenching: 2% taurine solution at a dosage of 1-5 mL per time and it was taken 2-6 times per day. The administration by mixed-feeding: 1-20 g/L of taurine solution was drunk freely. Lactating sows: each sow was administered with 5-500 g of taurine per day and the administration was in a single dose or in divided doses.

[0030] Specific operations: 40 litters of diseased newborn suckling piglets and sows thereof were selected and randomly divided into two groups equivalently. Control group: conventional supportive therapy (fluid infusion+antibiotic therapy+atropine sulfate); experimental group: treatment with taurine. Treatment results of the two groups are listed in Table 2. As seen from Table 2, a 10-day-aged survival rate and a weaned survival rate of the piglets in the experimental group are both significantly improved compared to those of the control group (P<0.05), and prognosis of the piglets is good.

TABLE-US-00002 TABLE 2 Dosage of Dosage 10-day- taurine of aged for the taurine survival Weaned lactating for the rate survival Group sows piglets (%) rate Prognosis Control group 0 0 8.3 3.2 Poor Experimental 100 g 0.2 g per 22.5 20.7 Good group per sow piglet per day per day

Embodiment 3 Use of Taurine in Prevention of the Porcine Epidemic Diarrhea of Weaned Piglets, Care Pigs and Finishing Pigs

[0031] Prevention of the porcine epidemic diarrhea on the weaned piglets, the care pigs and the finishing pigs with taurine was implemented by orally administering taurine to a swine herd, wherein the administration may be carried out by means of feeding in water or mixed feeding.

[0032] Drug administration time: a preventative administration was carried out during an epidemic period of the disease, or in autumn, winter and spring when the temperature was low and changed greatly, that is, in the season when the porcine epidemic diarrhea frequently occurs. Generally, the administration can be carried out for a long-term. In consideration of the use-cost, the drug administration time is preferably 3-30 days, and more preferably, 7-14 days. The effect is subject to an obvious time-effect relationship.

[0033] Drug administration dosage: 0.005-2% of taurine was added in daily-drinking water for the pigs to drink freely; or 0.01-5% of taurine was added into the feed, which is equivalent to a dosage of 50-10000 mg per kilogram of body weight per day of taurine, and the administration was in a single dose or in divided doses. The effect is subject to an obvious quantity-effect relationship.

[0034] Specific operations: 120 healthy weaned piglets, 120 healthy care pigs and 120 healthy finishing pigs were selected and randomly divided into two groups equivalently. Control group: conventional feeding; experimental group: conventional feeding+taurine (the dosage of taurine was 200 mg per kilogram of body weight per day). The prevention results of the two groups are listed in Table 3. As seen from Table 3, the disease incidence of the experimental group is lowered compared to that of the control group (P<0.05), and the survival rate of the experimental group is higher than that of the control group (P<0.05).

TABLE-US-00003 TABLE 3 Disease Survival incidence rate Group (%) (%) Prognosis Weaned piglets Control group 10 91.6 Normal Experimental group 6.6 96.6 Excellent Care pigs Control group 8.3 95.0 Normal Experimental group 3.3 96.6 Excellent Finishing pigs Control group 3.3 96.6 Normal Experimental group 0 98.3 Excellent

Embodiment 4 Use of Taurine in Treatment of the Porcine Epidemic Diarrhea of Weaned Piglets, Care Pigs and Finishing Pigs

[0035] Treatment of the porcine epidemic diarrhea on the weaned piglets, the care pigs and the finishing pigs with taurine was implemented by orally administering taurine to the pigs, wherein the administration may be carried out by means of feeding in water or mixed feeding.

[0036] Drug administration time: the administration was carried out from the day of occurrence of the disease, and in consideration of the use-cost, the drug administration time is preferably 3-30 days, and more preferably 7-14 days. The effect is subject to an obvious time-effect relationship.

[0037] Drug administration dosage: 0.1-2% of taurine was added in the daily-drinking water for the pigs to drink freely; or 0.2-5% of taurine was added into the feed, which is equivalent to a dosage of 100-10000 mg per kilogram of body weight per day of taurine, and the administration was in a single dose or in divided doses. The effect is subject to an obvious quantity-effect relationship.

[0038] Specific operations: 60 diseased weaned piglets, 60 diseased care pigs and 60 diseased finishing pigs were selected, and were randomly divided into two groups equivalently. Control group: conventional supportive therapy (fluid infusion+antibiotic therapy+atropine sulfate); experimental group: treatment with taurine (the dosage of taurine was 300 mg per kilogram of body weight per day). The treatment results of the two groups are listed in Table 4. As seen from Table 4, the survival rate of the experimental group is higher than that of the control group (P<0.05).

TABLE-US-00004 TABLE 4 Survival Group rate (%) Prognosis Weaned piglets Control group 86.6 General Experimental group 90.0 Good Care pigs Control group 90.0 General Experimental group 93.3 Good Finishing pigs Control group 96.6 General Experimental group 100 Good

Embodiment 5

[0039] According to methods as described in Embodiment 2 and Embodiment 4, treatment effects of taurine on porcine transmissible gastroenteritis, rotavirus diarrhea and pseudorabies viral diarrhea of the newborn piglets, the weaned piglets, the care pigs and the finishing pigs were tested, as listed in Table 5. The dosage of taurine for the newborn piglets was the same as that in Embodiment 2; and the dosage of taurine for the weaned piglets, the care pigs and the finishing pigs was the same as that in Embodiment 4. As seen from Table 5, taurine can be used for treatment of the porcine transmissible gastroenteritis and the rotavirus diarrhea, but the treatment effects are poorer than the treatment effect of taurine on the porcine epidemic diarrhea. However, taurine has no treatment effect on the pseudorabies viral diarrhea, for following reasons: in one aspect, a pseudorabies virus which belongs to a virus of genus porcine herpesvirus of family herpesviridae, is a virus having relatively strong resistibility among the family herpesviridae and still has infectivity after being treated with 0.5% phenol for 32 days; in another aspect, a category of the virus may be also a factor causing failure of the treatment effect. The porcine pseudorabies virus belongs to a DNA virus, whereas the porcine epidemic diarrhea virus, the porcine transmissible gastroenteritis virus and the rotavirus all belong to RNA viruses.

TABLE-US-00005 TABLE 5 Survival rate Group (%) Prognosis Transmissible Newborn Control group 9.8 Poor gastroenteritis piglets Experimental group 16.6 Good Weaned Control group 83.3 Poor piglets Experimental group 90 Good Care pigs Control group 90 Poor Experimental group 93.3 Good Finishing Control group 96.6 Poor pigs Experimental group 96.6 Good Rotavirus Newborn Control group 88.7 Poor diarrhea piglets Experimental group 93.6 Good Weaned Control group 90 General piglets Experimental group 96.6 Good Care pigs Control group 93.3 General Experimental group 96.6 Good Pseudorabies Newborn Control group 0 — viral piglets Experimental group 0 — diarrhea Care pigs Control group 46.6 General Experimental group 43.3 General