Abstract
A blood isolation and extraction method includes: providing a predetermined amount of blood; utilizing a platelet filter unit to filter the predetermined amount of blood to generate a filtered blood; utilizing a plasma separation unit to divide the filtered blood into a plasma layer and a blood cell layer for separating blood cells from blood plasma; and extracting the blood plasma from the plasma layer and the blood cells from the blood cell layer. In another embodiment, the blood isolation and extraction method further includes: providing a platelet-washing unit to wash the platelet filter unit with a solution to produce a platelet solution; and mixing the platelet solution with the blood plasma to produce a platelet and plasma mixed solution.
Claims
1. A blood isolation and extraction method comprising: providing a predetermined amount of blood; utilizing a platelet filter unit to filter the predetermined amount of blood to generate a filtered blood; utilizing a plasma separation unit to divide the filtered blood into a plasma layer and a blood cell layer for separating blood cells from blood plasma; and extracting the blood plasma from the plasma layer and the blood cells from the blood cell layer.
2. The blood isolation and extraction method as defined in claim 1, wherein the predetermined amount of blood is supplied by a blood supply unit or an injector.
3. The blood isolation and extraction method as defined in claim 1, wherein the platelet filter unit includes a platelet adsorption net filter or a platelet adsorption plate.
4. The blood isolation and extraction method as defined in claim 3, wherein the platelet adsorbing net filter or the platelet adsorption plate is made of platelet adsorption resin.
5. The blood isolation and extraction method as defined in claim 1, wherein the plasma separation unit includes a blood cell adsorbent, a blood cell adsorption material or a blood cell adsorption gel.
6. A blood isolation and extraction method comprising: providing a predetermined amount of blood; utilizing a platelet filter unit to filter the predetermined amount of blood to generate a filtered blood; washing the platelet filter unit with a solution to produce a platelet solution; utilizing a plasma separation unit to divide the filtered blood into a plasma layer and a blood cell layer for separating blood cells from blood plasma; and mixing the platelet solution with the blood plasma retrieved from the plasma layer to produce a platelet and plasma mixed solution.
7. The blood isolation and extraction method as defined in claim 6, wherein the predetermined amount of blood is supplied by a blood supply unit or an injector.
8. The blood isolation and extraction method as defined in claim 6, wherein the platelet filter unit includes a platelet adsorption net filter or a platelet adsorption plate.
9. The blood isolation and extraction method as defined in claim 8, wherein the platelet adsorbing net filter or the platelet adsorption plate is made of platelet adsorption resin.
10. The blood isolation and extraction method as defined in claim 6, wherein the plasma separation unit includes a blood cell adsorbent, a blood cell adsorption material or a blood cell adsorption gel.
11. The blood isolation and extraction method as defined in claim 6, wherein the solution is a buffer solution.
12. A blood isolation and extraction device comprising: a platelet filter unit provided to filter a predetermined amount of blood to generate a filtered blood; and a plasma separation unit provided to divide the filtered blood into a plasma layer and a blood cell layer for separating blood cells from blood plasma; wherein the blood plasma is capable of retrieving from the plasma layer and the blood cells are capable of retrieving from the blood cell layer.
13. The blood isolation and extraction device as defined in claim 12, wherein a platelet-washing unit is further provided to wash the platelet filter unit with a solution to produce a platelet solution.
14. The blood isolation and extraction device as defined in claim 13, wherein the platelet-washing unit includes a buffer dispenser.
15. The blood isolation and extraction device as defined in claim 13, wherein the solution is a buffer solution.
16. The blood isolation and extraction device as defined in claim 12, wherein the predetermined amount of blood is supplied by a blood supply unit or an injector.
17. The blood isolation and extraction device as defined in claim 12, wherein the platelet filter unit includes a platelet adsorption net filter or a platelet adsorption plate.
18. The blood isolation and extraction device as defined in claim 17, wherein the platelet adsorbing net filter or the platelet adsorption plate is made of platelet adsorption resin.
19. The blood isolation and extraction device as defined in claim 12, wherein the plasma separation unit includes a blood cell adsorbent, a blood cell adsorption material or a blood cell adsorption gel.
20. The blood isolation and extraction device as defined in claim 12, wherein the plasma separation unit includes a tube to contain the plasma layer and the blood cell layer formed in the filtered blood.
Description
BRIEF DESCRIPTION OF THE DRAWINGS
[0044] The present invention will become more fully understood from the detailed description given hereinbelow and the accompanying drawings which are given by way of illustration only, and thus are not limitative of the present invention, and wherein:
[0045] FIG. 1 is a flowchart of a blood isolation and extraction method in accordance with a first preferred embodiment of the present invention.
[0046] FIG. 2 is a schematic block diagram of a blood isolation and extraction device in accordance with a first preferred embodiment of the present invention.
[0047] FIG. 3 is a flowchart of a blood isolation and extraction method in accordance with a second preferred embodiment of the present invention.
[0048] FIG. 4 is a schematic block diagram of a blood isolation and extraction device in accordance with a second preferred embodiment of the present invention.
[0049] FIG. 5 is a schematic block diagram of a blood isolation and extraction device in accordance with a third preferred embodiment of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
[0050] It is noted that a blood isolation and extraction method and device thereof in accordance with the preferred embodiment of the present invention is suitable for manufacturing various whole blood products and related products thereof, including packed blood cells, fresh frozen plasma, cryoprecipitate, platelet concentrate, leukocyte concentrate and related products thereof for example, which are not limitative of the present invention. The blood isolation and extraction method and device thereof in accordance with the preferred embodiment of the present invention can also be applicable to various medical research, medical treatment (e.g. Achilles tendon rupture or arthritis treatment), medical cosmetology (e.g. baldness, wrinkle or collagen treatment), medical rehabilitation (e.g. plastic surgery or dental implant surgery), pharmaceutical products or related industries thereof, which are not limitative of the present invention.
[0051] FIG. 1 shows a flowchart of a blood isolation and extraction method in accordance with a first preferred embodiment of the present invention. FIG. 2 shows a schematic block diagram of a blood isolation and extraction device in accordance with the first preferred embodiment of the present invention, corresponding to the blood isolation and extraction method shown in FIG. 1. Referring now to FIGS. 1 and 2, the blood isolation and extraction device 1 in accordance with the first preferred embodiment of the present invention includes a material supply unit 10 (e.g. pump, injector or the like), a platelet filter unit 11 (e.g. platelet filter device) and a plasma separation unit 13 (e.g. plasma separation device) which can meet gamma radiation sterilization and biocompatibility requirement.
[0052] Still referring to FIGS. 1 and 2, by way of example, the material supply unit 10 connects the platelet filter unit 11 with a first conduit or pipeline and the platelet filter unit 11 further connects the plasma separation unit 13 with a second conduit or pipeline. The material supply unit 10 is suitably installed with a blood-drawing unit 101 or other blood-collecting devices (e.g., venipuncture syringes or test tubes).
[0053] With continued to FIGS. 1 and 2, the blood isolation and extraction method in accordance with the first preferred embodiment of the present invention includes the step S1: automatically, semi-automatically or manually operating the material supply unit 10 to supply a predetermined amount of blood (i.e. whole blood) to the platelet filter unit 11. By way of example, the material supply unit 10 includes an injector, a syringe or a pump device to operate the blood-drawing unit 101 so as to supply the blood to the platelet filter unit 11. In addition, the material supply unit 10 further includes a server motor or the like to pump the blood.
[0054] With continued to reference FIGS. 1 and 2, the blood isolation and extraction method in accordance with the first preferred embodiment of the present invention includes the step S2: automatically, semi-automatically or manually operating the platelet filter unit 11 to filter the predetermined amount of blood to generate a filtered blood. By way of example, after the predetermined amount of blood is pumped to pass through the platelet filter unit 11 with a predetermined pressure, platelets are selectively adsorbed and remained on a surface portion of the platelet filter unit 11. Thereafter, the platelets will not be contained in the filtered blood. Advantageously, the blood isolation and extraction method of the present invention can avoid problems of rapid loss of filtered blood pressure, velocity drop of filtered blood flow and choke of filter holes in filtering operation.
[0055] With continued to reference FIGS. 1 and 2, by way of example, the platelet filter unit 11 includes a platelet adsorption net filter or a platelet adsorption plate. The platelet adsorbing net filter includes a plurality of apertures performed as filtering holes and is made of platelet adsorption resin or other equivalent materials. The platelet adsorption plate is also made of platelet adsorption resin other equivalent materials. The material of platelet adsorption resin has a function of platelet adsorption and a high degree of recovery of platelets.
[0056] By way of example, the platelet adsorption resin or resin is modified by a surface modifier selected from modifiers containing phosphatidyl choline (pc) derivatives or zwitterionic dopamine derivatives which have highly stable and water absorbability. The zwitterionic structure can avoid bonding with other metal bonds, polynucleotide and proteins. In addition, the zwitterionic structure has a high degree of hydrophile even though it can absorb four times of water to contain proteins after dried. Advantageously, it is hard to be permeated or absorbed to prevent clot reaction or hemolytic reaction.
[0057] With continued to reference FIGS. 1 and 2, the blood isolation and extraction method in accordance with the first preferred embodiment of the present invention includes the step S3: transferring the filtered blood to the plasma separation unit 13 and automatically, semi-automatically or manually operating the plasma separation unit 13 to divide the filtered blood into a plasma layer and a blood cell layer in a predetermined tube for separating blood cells (i.e. corpuscles) from blood plasma. Generally, the plasma layer is an upper layer (i.e. creamy yellow) relatively while the blood cell layer is a lower layer.
[0058] With continued to reference FIGS. 1 and 2, by way of example, the plasma separation unit 13 includes a blood cell adsorbent, a blood cell adsorption gel or other blood cell adsorption materials. The blood cell adsorbent or the blood cell adsorption gel is cured on sponges, foam rubber. hemostatic cotton or surfaces of other fillers which are preferably contained in a cone container.
[0059] With continued to reference FIGS. 1 and 2, the blood isolation and extraction method in accordance with the first preferred embodiment of the present invention includes the step S4: automatically, semi-automatically or manually operating a tool (e.g. pipette or other equivalent tools) to draw out the liquids of plasma layer and blood cell layer for extracting the blood plasma from the plasma layer and the blood cells from the blood cell layer. With continued to reference FIGS. 1 and 2, by way of example, the blood plasma is stored in a plasma storage unit 131 (i.e. first sterilized container or other equivalent devices) while the blood cells are separately stored in a blood cell storage unit 132 (i.e. second sterilized container or other equivalent devices).
[0060] FIG. 3 shows a flowchart of a blood isolation and extraction method in accordance with a second preferred embodiment of the present invention. FIG. 4 shows a schematic block diagram of a blood isolation and extraction device in accordance with the second preferred embodiment of the present invention, corresponding to the blood isolation and extraction method shown in FIG. 3.
[0061] Referring now to FIGS. 3 and 4, the blood isolation and extraction device 1′ in accordance with the second preferred embodiment of the present invention includes a material supply unit 10, a blood-drawing unit 101, a buffer supply unit 102, a platelet filter unit 11 and a plasma separation unit 13 which can meet gamma radiation sterilization and biocompatibility requirement.
[0062] Still referring to FIGS. 3 and 4, by way of example, the blood-drawing unit 101 and the buffer supply unit 102 connect the material supply unit 10 with a first conduit, the material supply unit 10 further connects the platelet filter unit 11 with a second conduit and the platelet filter unit 11 further connects the plasma separation unit 13 with a third conduit. The material supply unit 10 is suitably installed with a blood supply source or other blood-collecting devices.
[0063] With continued to FIGS. 3 and 4, the blood isolation and extraction method in accordance with the second preferred embodiment of the present invention includes the step S1: automatically, semi-automatically or manually operating the material supply unit 10 to supply a predetermined amount of blood (i.e. whole blood) to the platelet filter unit 11. By way of example, the material supply unit 10 includes an injector, a syringe or a pump device to operate the blood-drawing unit 101 so as to supply the blood to the platelet filter unit 11. In addition, the material supply unit 10 further includes a server motor or the like to pump the blood.
[0064] With continued to reference FIGS. 3 and 4, the blood isolation and extraction method in accordance with the second preferred embodiment of the present invention includes the step S2: automatically, semi-automatically or manually operating the platelet filter unit 11 to filter the predetermined amount of blood to generate a filtered blood. By way of example, after the predetermined amount of blood is pumped to pass through the platelet filter unit 11 with a predetermined pressure, platelets are selectively adsorbed and remained on a surface portion of the platelet filter unit 11. Thereafter, the platelets will not be contained in the filtered blood. Advantageously, the blood isolation and extraction method of the present invention can avoid problems of rapid loss of filtered blood pressure, velocity drop of filtered blood flow and choke of filter holes in filtering operation.
[0065] With continued to reference FIGS. 3 and 4, the blood isolation and extraction method in accordance with the second preferred embodiment of the present invention includes the step S3: automatically, semi-automatically or manually operating the material supply unit 10 and the buffer supply unit 102 to wash or to flush the platelet filter unit 11 with a solution (or a buffer solution) at least one or several times to produce a platelet solution which contains platelets remained on the platelet filter unit 11. By way of example, the platelet solution is stored in a platelet storage unit 111 (i.e. sterilized container). In platelet washing operation, the material supply unit 10 has ceased to supply the blood from the blood-drawing unit 101.
[0066] With continued to reference FIGS. 3 and 4, by way of example, the material supply unit 10 includes an injector, a syringe or a pump device to operate the buffer supply unit 102 to form a platelet-washing unit which can wash or flush the platelets on the platelet filter unit 11 with the buffer solution (e.g. phosphate buffer solution, PBS).
[0067] With continued to reference FIGS. 3 and 4, the blood isolation and extraction method in accordance with the second preferred embodiment of the present invention includes the step S4: transferring the filtered blood to the plasma separation unit 13 and automatically, semi-automatically or manually operating the plasma separation unit 13 to divide the filtered blood into a plasma layer and a blood cell layer in a predetermined tube for separating blood cells from blood plasma. Generally, the plasma layer is an upper layer (i.e. creamy yellow) relatively while the blood cell layer is a lower layer.
[0068] With continued to reference FIGS. 3 and 4, the blood isolation and extraction method in accordance with the second preferred embodiment of the present invention includes the step S5: automatically, semi-automatically or manually mixing the platelet solution with the blood plasma retrieved from the plasma layer to produce a platelet and plasma mixed solution. By way of example, the platelet and plasma mixed solution is stored in a mixed solution storage unit 112 (i.e. sterilized container).
[0069] FIG. 5 shows a schematic block diagram of a blood isolation and extraction device in accordance with a third preferred embodiment of the present invention. Referring to FIG. 5, the blood isolation and extraction device 1″ in accordance with the second preferred embodiment of the present invention includes a material supply unit 10, a blood-drawing unit 101, a platelet filter unit 11, a platelet-washing unit 12 and a plasma separation unit 13 which can meet gamma radiation sterilization and biocompatibility requirement.
[0070] Still referring to FIG. 5, by way of example, the platelet-washing unit 12 connects the platelet filter unit 11 with a conduit. In platelet washing operation, the platelet-washing unit 12 is automatically, semi-automatically or manually operated to wash or to flush the platelet filter unit 11 with a solution (or a buffer solution) at least one or several times to produce a platelet solution. Synchronously, the material supply unit 10 has ceased to supply the blood from the blood-drawing unit 101.
[0071] Although the invention has been described in detail with reference to its presently preferred embodiment, it will be understood by one of ordinary skills in the art that various modifications can be made without departing from the spirit and the scope of the invention, as set forth in the appended claims.
