METHOD FOR TREATING RAW MATERIALS OF PLANT-DERIVED FOOD

Abstract

The invention discloses a method for treating raw materials of plant-derived food, wherein the method comprises the following steps: mixing raw materials of plant-derived food with a solution containing photosensitizer to obtain a mixing liquid; carrying out light treatment of the mixing liquid; collecting raw materials in the mixing liquid subjected to light treatment, and performing drying treatment of the raw materials. The method can effectively realize sterilization effect, especially effectively reduce spore contamination, so as to ensure the safety of food and extend the shelf life; besides, the method can keep the original color, flavor, taste and nutrition contents of plant-derived food, and improve the edible value of plant-derived food.

Claims

1. A method for treating raw materials of plant-derived food comprises: Mixing raw materials of plant-derived food with a solution containing photosensitizer to obtain a mixing liquid; Carrying out light treatment of the mixing liquid; Collecting raw materials in the mixing liquid subjected to light treatment, and performing drying treatment of the raw materials.

2. The method according to claim 1 is characterized in that pepper, onion and garlic are used as the raw materials of plant-derived food.

3. The method according to claim 1 is characterized in that the raw materials of plant-derived food are crushed to particles with sizes of 0.5˜5.0 cm before the mixing treatment.

4. The method according to claim 1 is characterized in that sodium copper chlorophyllin, riboflavin or curcumin is used as the photosensitizer.

5. The method according to claim 1 is characterized in that when sodium copper chlorophyllin is used as the photosensitizer, the mass ratio of the raw materials of plant-derived food to the photosensitizer in the mixing liquid is (1˜30): 1 based on the total volume of the mixing liquid, preferably (10˜20): 1; When curcumin is used as the photosensitizer, the mass ratio of the raw materials of plant-derived food to the photosensitizer in the mixing liquid is (10,000˜20,000): 1 based on the total volume of the mixing liquid, preferably (14,000˜16,000): 1.

6. The method according to claim 1 is characterized in that the wavelength, irradiation time and power adopted for the light treatment are 420˜600 nm, 10˜80 min and 100˜200 W respectively; When sodium copper chlorophyllin is used as the photosensitizer, the wavelength and irradiation time adopted for the light treatment are 420˜530 nm and 50˜70 min respectively; When curcumin is used as the photosensitizer, the wavelength and irradiation time adopted for the light treatment are 460˜520 nm and 10˜20 min respectively.

7. The method according to claim 1 is characterized in that the raw materials of plant-derived food are cleaned before the mixing treatment.

Description

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0022] The scheme of the invention will be explained in the embodiment below. Those skilled in the art will understand that the following embodiment is only used to illustrate the invention, and should not be regarded as limiting the scope of the invention. If the specific technology or conditions are not indicated in the embodiment, the technology or conditions described in the literature in this field or the product specification shall be followed. The adopted reagents or instruments, with manufacturer not indicated, are conventional products can be obtained through purchase in the market.

Embodiment 1

[0023] 1. Sample Treatment:

[0024] (1) Experiment 1: clean 100 g of unsterilized onion, garlic and white pepper, respectively, crush them to particles with sizes of 0.5˜5.0 cm, dissolve into 1 L of 0.9% normal saline, and shake fully and evenly to obtain a liquid with the three kinds of raw materials for later use.

[0025] Experiment 2: dry and pulverize the onion, garlic and white pepper crushed in Experiment 1 to obtain onion powder, garlic powder and white pepper powder, dissolve the three kinds of powders into 0.9% normal saline, and shake fully and evenly to obtain a liquid with the three kinds of powders for later use.

[0026] Preparation of photo sensitizer: prepare 8 mmol/L sodium copper chlorophyllin solution and 35 μmol/L curcumin solution respectively from light.

[0027] (2) Experiment 1 and Experiment 2 are carried out by the following four groups respectively:

[0028] Light experiment (PDT) group: different types of photosensitizers were added in each group of experiments; light was applied after incubation from light.

[0029] Single photosensitizer control group: the grouping method was the same as PDT group; no light was applied after incubation from light.

[0030] Single light control group: no photosensitizer was added; light was applied together with PDT group after incubation from light.

[0031] Blank control group: no photosensitizer was added; no light was applied after incubation from light.

[0032] Samples were loaded according to the sample loading method in Table 1, with the incubation time of 45 min, and then they were placed in a 150 W light source (the wavelength of sodium copper chlorophyllin experimental group was 520 nm, and that of curcumin experimental group was 520 nm), wherein the sodium copper chlorophyllin experimental group was lighted for 63.84 min, while the curcumin experimental group was lighted for 15 min. The concentration of photosensitizer added in the single photosensitizer control group was the same as that of photosensitizer added in the light experiment group, but the single photosensitizer control group was not subjected to light treatment; for the single light control group, no photosensitizer was added, but the light conditions were the same as those of the light experimental group; the blank control group was not subjected to any treatment.

TABLE-US-00001 TABLE 1 Ratio of photosensitizer to raw material/powder liquid Light Single Single light Blank Reagent experiment photosensitizer control control (mL) group control group group group Raw material/ 2 2 2 2 powder liquid Photosensitizer 2 2 0 0 Sterile water 0 0 2 2

[0033] (3) After treatment, suck up 25 mL of liquid from each group and dilute with 225 mL of sterile normal saline to obtain 10.sup.−1 diluted solution, and so on to obtain 10.sup.−2˜10.sup.−7 diluted solution, fully mix them on a vortex shaker, select an appropriate dilution gradient, suck up 100 μL of bacterial solution and inoculate on a LB plate, coat evenly, and place in an incubator at 37° C. for 24 h; calculate the survival bacteria count after treatment in each group, and then calculate the survival rate of bacteria; set up three parallel controls in each group of experiments, with each experiment repeated for three times.

[00001]$\mathrm{Survival}\mathrm{rate}\mathrm{of}\mathrm{bacteria}\left(\%\right)=\frac{\mathrm{Total}\mathrm{bacterial}\mathrm{count}\mathrm{in}\mathrm{experimental}\mathrm{group}\left(\log \mathrm{CFU}/\mathrm{mL}\right)}{\mathrm{Total}\mathrm{bacterial}\mathrm{count}\mathrm{in}\mathrm{blank}\mathrm{control}\mathrm{group}\left(\log \mathrm{CFU}/\mathrm{mL}\right)}\times 100$

[0034] 2. Results and Analysis

[0035] In Experiment 1, after the original floras of white pepper, garlic and onion were sterilized by light, the bacteria count changed significantly, with the specific data shown in Table 2 and Table 3 (the survival bacteria count log CFU/mL is shown in the tables), showing that sterilization effect is better if sterilization treatment is performed before drying treatment.

TABLE-US-00002 TABLE 2 Sterilization for three kinds of powders by PDT treatment with sodium copper chlorophyllin taken as photosensitizer Sample name White pepper Garlic Onion (logCFU/mL) powder powder powder Light experiment 1.41 ± 0.089.sup.b 1.03 ± 0.550.sup.b 1.12 ± 0.170.sup.b group Single light 5.19 ± 0.008.sup.ab 4.87 ± 0.103.sup.a 5.13 ± 0.012.sup.a control group Single sodium copper 5.24 ± 0.120.sup.a 4.75 ± 0.037.sup.a 5.09 ± 0.064.sup.a chlorophyllin group Blank control group 5.39 ± 0.014.sup.a 4.90 ± 0.008.sup.a 5.18 ± 0.049.sup.a

TABLE-US-00003 TABLE 3 Sterilization for three kinds of powders by PDT treatment with curcumin taken as photosensitizer Sample name White pepper Garlic Onion (logCFU/mL) powder powder powder Light experiment 1.12 ± 0.033.sup.c 0.95 ± 0.000.sup.c 1.05 ± 0.025.sup.b group Single light control 5.89 ± 0.008.sup.b 4.89 ± 0.103.sup.b 5.37 ± 0.028.sup.a group Single curcumin 5.97 ± 0.029.sup.b 4.97 ± 0.073.sup.b 5.32 ± 0.028.sup.a control group Blank control group 6.02 ± 0.014.sup.a 5.09 ± 0.008.sup.a 5.46 ± 0.049.sup.a

[0036] In Experiment 2, after the original floras of white pepper, garlic and onion were sterilized by light, the bacteria count in the garlic powder did not change significantly, while the bacteria count in the onion powder and the white pepper decreased, but the sterilization efficiency was low (less than 10%), with the specific data shown in Table 4 and Table 5 (the survival rate of bacterial % is shown in the tables), showing that sterilization effect is worse if sterilization treatment is performed after drying treatment.

TABLE-US-00004 TABLE 4 Sterilization for three kinds of powders by PDT treatment with sodium copper chlorophyllin taken as photosensitizer Sample name White pepper Garlic Onion (logCFU/mL) powder powder powder Light experiment group 91.81 ± 2.65.sup.b 73.20 ± 28.99.sup.a 89.92 ± 0.56.sup.b Single light control group 94.26 ± 0.23.sup.ab 93.39 ± 5.44.sup.a 95.49 ± 0.93.sup.a Single sodium copper 98.67 ± 3.55.sup.a 92.52 ± 1.98.sup.a 96.18 ± 2.09.sup.a chlorophyllin group

TABLE-US-00005 TABLE 5 Sterilization for three kinds of powders by PDT treatment with curcumin taken as photosensitizer Sample name White pepper Garlic Onion (logCFU/mL) powder powder powder Light experiment 95.43 ± 0.96.sup.c 97.23 ± 0.00.sup.a 96.56 ± 0.81.sup.b group Single light 94.26 ± 0.23.sup.c 93.39 ± 5.44.sup.a 95.49 ± 0.93.sup.b control group Single curcumin 97.67 ± 0.88.sup.b 92.25 ± 3.84.sup.a 96.35 ± 0.93.sup.b control group

[0037] In the description of this specification, the terms “one embodiment”, “some embodiments”, “example”, “specific example” or “some examples” mean that the specific features, structures, materials or characteristics described in combination with the embodiment or the example are included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms are not necessarily for the same embodiment or example. Furthermore, the described specific features, structures, materials or characteristics may be combined in any one or more embodiment (s) or example (s) in a suitable manner. In addition, those skilled in the art can combine the different embodiments or examples and the features of the different embodiments or examples described in this specification without contradicting each other.

[0038] Although the embodiment of the invention has been shown and described above, the above embodiment is illustrative and should not be construed as limitation of the invention, and those skilled in the art can make changes, modifications, substitutions and variations to the above embodiment within the scope of the invention.