ACNE REMOVING SKIN CARE PRODUCT AND PREPARATION METHOD THEREOF

Abstract

Disclosed are an acne removal composition, a skin care product and a preparation method thereof, wherein the acne removal composition is made from Garcinia mangostana L pericarp, thyme and Fructus aurantii.

Claims

1. An acne-removing composition, consisted of mangosteen pericarp, thyme and Aurantii fructus.

2. The acne-removing composition of claim 1, wherein the weight ratio of mangosteen pericarp, thyme and Fructus aurantii is (6 to 8):(1 to 2):(1 to 2).

3. An acne-removing skincare product, comprising the acne-removing composition of claim 1.

4. The acne-removing skincare product of claim 3, further comprises at least one of Hedyotis diffusa Willd., Radix Salvia miltiorrhiza, Caulis Lonicerae, Gleditsiae sinensis seed and tea tree essential oil.

5. The acne-removing skincare product of claim 3, further comprises acne-removing compound preparation A100 and tea tree essential oil.

6. The acne-removing skincare product of claim 5, wherein the weight ratio of the acne-removing composition of claim 1, Acne-Removing Compound Preparation A100, and tea tree essential oil is (1 to 5):(2 to 10):(0.05 to 0.1).

7. The acne-removing skincare product according to claim 3, further comprises at least one of solvent, thickener, conditioner, humectant, pH regulator, surfactant and freshener.

8. The acne-removing skincare product of claim 7, wherein the solvent is at least one of water, propylene glycol, butylenes glycol, glycerol and pentylene glycol; the thickener is at least one of Carbomer and SEPINOV EMT-10; the conditioner is at least one of allantoin, vitamin A palmitate, dipotassium glycyrrhizinate, Centella asiatica extract, nicotinamide, octanoylglycine, quaternary ammonium salt-73, salicylic acid, and octanoylsalicylic acid; the humectant is at least one of ethylhexylglycerin, butylenes glycol, 1,2-pentylene glycol, glycerol, propylene glycol, sodium hyaluronate, D-panthenol and PCA-Na; the pH regulator is at least one of sodium hydroxide, potassium hydroxide, arginine, citric acid and sodium citrate; the surfactant is at least one of tween-20, PEG-40 hydrogenated castor oil and PEG-60 hydrogenated castor oil; and the freshener is at least one of cooling complex, menthol, mint essential oil and menthol lactate.

9. The acne-removing skincare product of claim 7, comprising: TABLE-US-00015 the acne-removing composition 1 wt %-5 wt % of claim 1 or claim 2 acne-removing compound  2 wt %-10 wt % preparation A100 tea tree essential oil 0.06 wt % carbomer U21 0.45 wt % SEPINOV EMT-10  1.2 wt % allantoin 0.1-0.3 wt %   vitamin A palmitate   0 wt %-0.5 wt % dipotassium glycyrrhizinate 0-0.3 wt % Centella asiatica extract 0-0.5 wt % nicotinamide 0.5 wt %-3 wt %   butylenes glycol 1 wt %-5 wt % pentylene glycol 0.5 wt %-3 wt %   sodium hydroxide 0.15 wt % tween-20   0-1 wt % cooling complex 0-0.1 wt % propylene glycol   0-8 wt % water making up to 100 wt %.

10. A method for preparing an acne-removing skincare product, comprising the following steps: 1) carbomer U21, allantoin and allantoin are taken and dissolved completely by adding water, then heated to 70° C.-95° C. to obtain a first reaction solution. 2) acne-removing compound preparation A100 and the acne-removing composition of claim 1 or 2 are dissolved by adding water and propylene glycol, respectively, then added into the first reaction solution at 80° C., mixed to dissolved completely to obtain a second reaction solution; 3) sodium hydroxide is added to the second reaction solution, and mixed evenly to obtain a third reaction solution; 4) butylenes glycol and vitamin A palmitate are added to the third reaction solution and mixed, the temperature is reduced to 45° C., and a forth reaction solution is obtained; 5) pentylene glycol, cooling complex and tea tree essential oil are mixed and dissolved, then added into the forth reaction solution, mixed evenly, to obtain a fifth reaction solution; and 6) dipotassium glycyrrhizinate, Centella asiatica extract and nicotinamide are dissolved by adding water, then added into the fifth reaction solution, and mixed evenly.

Description

DETAILED DESCRIPTION OF EMBODIMENTS

[0098] Hereinafter, the technical solutions in the examples of the present invention will be described clearly and completely in conjunction with examples of the present invention. It is apparent that the described examples are merely a part of the examples of the present invention rather than all. Based on the examples of the present invention, all other examples obtained by a person skilled in the art without creative work are within the scope of the present invention.

[0099] To further understand the present invention, detailed descriptions are provided in combination with the following examples.

Example 1: An Acne-Removing Composition of the Present Invention

[0100] 1. Mangosteen pericarp, thyme and Fructus aurantii were crushed respectively, passed through a 60-mesh sieve, and mixed in a ratio of 80 g mangosteen pericarp, 20 g thyme and 20 g Fructus aurantii;

[0101] 2. reflux extraction was performed for 2.5 h by using 95% ethanol-water solution in a amount of 12 folds by weight of the raw materials;

[0102] 3. the above extraction solution was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 72 g concentrated solution with a specific gravity of 1.08;

[0103] 4. recycled ethanol-water solution was adjusted to a concentration of 80%, added to the concentrated solution at a volume ratio of 6:1 (ethanol-water solution: concentrated solution); stirred and dissolved at 80° C. for 2 h, and refrigerated overnight at 4° C. for 18 h;

[0104] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 40 g product with a specific gravity of 1.08;

[0105] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 1BV, the amount of 40% ethanol-water solution used is 1BV, and the amount of 80% ethanol-water solution used is 4BV;

[0106] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.06, followed by spay drying to obtain 5.04 g.

Example 2: An Acne-Removing Composition of the Present Invention

[0107] 1. Mangosteen pericarp, thyme and Fructus aurantii were crushed respectively, passed through a 60-mesh sieve, and mixed in a ratio of 60 g mangosteen pericarp, 10 g thyme and 10 g Fructus aurantii;

[0108] 2. reflux extraction was performed for 1 h by using 60% ethanol-water solution in an amount of 8 folds by weight of the raw materials;

[0109] 3. the above extract was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 106 g concentrated solution with a specific gravity of 1.08;

[0110] 4. recycled ethanol-water solution was adjusted to a concentration of 60%, added to the concentrated solution at a volume ratio of 4:1 (ethanol-water solution:concentrated solution); stirred and dissolved at 50° C. for 0.5 h, and refrigerated overnight at 0° C. for 12 h;

[0111] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 76 g product with a specific gravity of 1.08;

[0112] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 2BV, the amount of 40% ethanol-water solution used is 2BV, and the amount of 80% ethanol-water solution used is 2BV;

[0113] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.06, followed by spay drying to obtain 3.2 g.

Example 3: An Acne-Removing Composition of the Present Invention

[0114] 1. Mangosteen pericarp, thyme and Fructus aurantii were crushed respectively, passed through a 60-mesh sieve, and mixed in a ratio of 80 g mangosteen pericarp, 10 g thyme and 10 g Fructus aurantii;

[0115] 2. reflux extraction was performed for 2 h by using 75% ethanol-water solution in a amount of 10 folds by weight of the raw materials;

[0116] 3. the above extract was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 106 g concentrated solution with a specific gravity of 1.06;

[0117] 4. recycled ethanol-water solution was adjusted to a concentration of 70%, added to the concentrated solution at a volume ratio of 5:1 (ethanol-water solution: concentrated solution); stirred and dissolved at 60° C. for 1 h, and refrigerated overnight at 2° C. for 15 h;

[0118] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 76 g product with a specific gravity of 1.08;

[0119] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 1BV, the amount of 40% ethanol-water solution used is 2BV, and the amount of 80% ethanol-water solution used is 3BV;

[0120] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.06, followed by spay drying to obtain 4.4 g.

Comparative Example 1

[0121] 1. 100 g Mangosteen pericarp was crushed, and passed through a 60-mesh sieve;

[0122] 2. reflux extraction was performed for 2 h by using 75% ethanol-water solution in a amount of 10 folds by weight of the raw materials;

[0123] 3. the above extract was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 62 g concentrated solution with a specific gravity of 1.06;

[0124] 4. recycled ethanol-water solution was adjusted to a concentration of 70%, added to the concentrated solution at a volume ratio of 5:1 (ethanol-water solution:concentrated solution); stirred and dissolved at 60° C. for 1.5 h, and refrigerated overnight at 2° C. for 15 h;

[0125] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 54 g product with a specific gravity of 1.08;

[0126] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 1BV, the amount of 40% ethanol-water solution used is 2BV, and the amount of 80% ethanol-water solution used is 3BV;

[0127] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.08, followed by spay drying to obtain 4.0.

Comparative Example 2

[0128] 1. 100 g thyme was crushed, and passed through a 60-mesh sieve;

[0129] 2. reflux extraction was performed for 2 h by using 75% ethanol-water solution in a amount of 10 folds by weight of the raw materials;

[0130] 3. the above extract was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 52 g concentrated solution with a specific gravity of 1.06;

[0131] 4. recycled ethanol-water solution was adjusted to a concentration of 70%, which was added to the concentrated solution at a volume ratio of 5:1 (ethanol-water solution: concentrated solution); stirred and dissolved at 60° C. for 1.5 h, and refrigerated overnight at 2° C. for 15 h;

[0132] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 38 g product with a specific gravity of 1.08;

[0133] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 1BV, the amount of 40% ethanol-water solution used is 2BV, and the amount of 80% ethanol-water solution used is 3BV;

[0134] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.06, followed by spay drying to obtain 1.2 g.

Comparative Example 3

[0135] 1. 100 g Fructus aurantii was crushed, and passed through a 60-mesh sieve;

[0136] 2. reflux extraction was performed for 2 h by using 75% ethanol-water solution in a amount of 10 folds by weight of the raw materials;

[0137] 3. the above extract was filtered through 100-mesh filter cloth to remove the residue, concentrated under reduced pressure (temperature 60° C.-80° C., vacuum degree≧0.08 Mpa, the same below) to obtain 70 g concentrated solution with a specific gravity of 1.06;

[0138] 4. recycled ethanol-water solution was adjusted to a concentration of 70%, added to the concentrated solution at a volume ratio of 5:1 (ethanol-water solution:concentrated solution); stirred and dissolved at 60° C. for 1.5 h, and refrigerated overnight at 2° C. for 15 h;

[0139] 5. the refrigerated solution was filtered and the filtrate was concentrated under reduced pressure to obtain 58 g product with a specific gravity of 1.08;

[0140] 6. the concentrated solution was adsorbed by polyamide resins and eluted with water, 40% ethanol-water solution, 80% ethanol-water solution respectively, wherein the amount of water used is 1BV, the amount of 40% ethanol-water solution used is 2BV, and the amount of 80% ethanol-water solution used is 3BV;

[0141] 7. a fragment eluted by 80% ethanol-water solution was collected and concentrated under reduced pressure to a specific gravity of 1.05, followed by spay drying to obtain 1.8 g.

Experimental Example 1: Bacteriostasis Effect

1. Materials

[0142] 1.1 Test bacterial strain: Propionibacterium acnes (ATCC6919)
1.2 Chinese medicine composition samples: D1, D2, D3, D4, D5 and D6, stored at 4° C. for use, wherein samples D1 to D6 correspond to the products prepared by Examples 1, 2 and 3, and Comparative examples 1, 2 and 3, respectively.
1.3 Culture medium: Propionibacterium acnes culture medium (pH was adjusted to 6.6 to 7.0). Agar was added in 15 g per liter to the solid culture medium.

2. Methods

2.1 Preparation of Bacteria Suspension and Inoculation

[0143] 0.1 ml frozen deposit bacteria suspension was added to 5 ml Propionibacterium acnes culture medium and cultured at 37° C. under anaerobic condition for 2 days, thus the bacteria suspension for the experiment was obtained.

2.2 Preparation of Sample Solutions

[0144] Samples D1, D2, D3, D4, D5 and D6 were diluted in saline to the test concentration of 0.1%, followed by gradient dilution, respectively. In the first test, the gradient concentrations were 100% (original sample solution), 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56% and 0.78%, respectively. In the second test, the gradient diluted concentrations for second test were 10%, 8%, 6%, 4% and 2%, respectively.

2.3 Preparation of Bacteria Suspension

[0145] The bacteria suspension was diluted in Propionibacterium acnes culture medium to a final concentration of 10.sup.6 CFU/ml.

2.4 Culture and Result Judgement

[0146] 100 μL bacteria suspension and 100 μL sample solution were added to the well. The negative control without adding bacteria and the normal growth control without adding test solution were set at the same time. Each sample was performed in triplicate and the average was taken. Results were observed after anaerobic incubation at 37° C. for 48 h. The presence of turbidity was judged by naked eye and data were read out directly. The prerequisites for result judgement were: the growth control is well, there is no bacteria and the growth is clear for the blank control, and the growth of bacteria in other wells was inhibited with the increasing gradient concentrations of the drugs.

TABLE-US-00002 TABLE 1 Results of the first bacteriostasis test in gradient concentrations Gradient Concentrations D1 D2 D3 D4 D5 D6   50% − − − − − −   25% − − − − + +  12.5% − − − + + +  6.25% + + + + + + 3.125% + + + + + +  1.56% + + + + + +  0.78% + + + + + + Note: + means presence of bacteria growth; − means no bacteria growth.

TABLE-US-00003 TABLE 2 Results of the second bacteriostasis test by gradient concentrations Gradient Concentrations D1 D2 D3 D4 10%  − − − + 8% − + − + 6% + + + + 4% + + + + 2% + + + + 1% + + + + Note: + means presence of bacteria growth; − means no bacteria growth.

2.5 Results

[0147] It can be found from the results in Table 1 that the solutions from the three examples with a concentration of 0.1% all had good growth inhibition on Propionibacterium acnes when they were diluted to 12.5% or higher concentrations. However, in comparative samples, a desirable effect can be achieved when the concentration of the diluted solutions is 25% or higher for D4 and the concentration is 50% or higher for D5 and D6. It can be found from the results in Table 2, after verification, the bacteriostasis effect can be achieved by D1 and D3 at a concentration of 8%, and by D2 at a concentration of 10%.

[0148] It can be known from the above results that minimal addition of the composition product of the present invention in about 0.01% can achieve basically the inhibition effect on Propionibacterium acnes. Also, the composition of the present invention has a stronger bacteriostasis effect than the medicinal material used solely.

Example 4: An Acne-Removing Skincare Product of the Present Invention

[0149] Formula:

TABLE-US-00004 Acne-removing composition   2 wt % in example 1 Acne-removing compound   10 wt % preparation A100 Tea tree essential oil 0.06 wt % Carbomer U21 0.45 wt % SEPINOV EMT-10  1.2 wt % Allantoin 0.15 wt % Vitamin A palmitate  0.5 wt % Dipotassium glycyrrhizinate  0.2 wt % Nicotinamide   1 wt % Butylenes glycol   2 wt % Pentylene glycol  0.5 wt % Sodium hydroxide 0.15 wt % tween-20  0.6 wt % cooling complex 0.05 wt % Propylene glycol   6 wt % Water making up to 100 wt %

Preparation Method:

[0150] 1) Carbomer U21 and allantoin are taken and dissolved completely by adding water, then heated to 80° C. to obtain the first reaction solution.

[0151] 2) acne-removing compound preparation A100 and the acne-removing composition of the present invention are dissolved by adding water and propylene glycol, respectively, then added into the first reaction solution, stirred at 80° C. and homogenized to dissolve the raw material completely to obtain the second reaction solution;

[0152] 3) sodium hydroxide is added to the second reaction solution, and mixed evenly at 80° C. to obtain the third reaction solution;

[0153] 4) Butylenes glycol and vitamin A palmitate are added to the third reaction solution and mixed evenly, the temperature is reduced to 45° C., and the forth reaction solution is obtained;

[0154] 5) Pentylene glycol, cooling complex and tea tree essential oil are mixed, heated to 85° C., stirred to be dissolved completely, then added into the forth reaction solution, mixed evenly, to obtain the fifth reaction solution; and

[0155] 6) Dipotassium glycyrrhizinate, and nicotinamide are dissolved by adding water, then added into the fifth reaction solution, and mixed evenly.

Example 5: An Acne-Removing Skincare Product of the Present Invention

[0156] Formula:

TABLE-US-00005 The acne-removing composition   2 wt % in example 2 Acne-removing compound   10 wt % preparation A100 Tea tree essential oil 0.06 wt % Carbomer U21 0.45 wt % SEPINOV EMT-10  1.2 wt % Allantoin 0.15 wt % Dipotassium glycyrrhizinate  0.2 wt % Nicotinamide   1 wt % Butylenes glycol   2 wt % Pentylene glycol  0.5 wt % Sodium hydroxide 0.15 wt % tween-20  0.6 wt % cooling complex 0.05 wt % Propylene glycol   6 wt % Water making up to 100 wt %

Preparation Method:

[0157] 1) Carbomer U21, allantoin and SEPINOV EMT-10 are taken and dissolved completely by adding water, then heated to 80° C. to obtain the first reaction solution.

[0158] 2) acne-removing compound preparation A100 and the acne-removing composition of the present invention are dissolved by adding water and propylene glycol, respectively, then added into the first reaction solution, stirred at 80° C. and homogenized to dissolve the raw material completely to obtain the second reaction solution;

[0159] 3) sodium hydroxide is added to the second reaction solution, and mixed evenly at 80° C. to obtain the third reaction solution;

[0160] 4) Butylenes glycol is added to the third reaction solution and mixed evenly, the temperature is reduced to 45° C., and the forth reaction solution is obtained;

[0161] 5) Pentylene glycol, cooling complex and tea tree essential oil are mixed, stirred to be dissolved completely, then added into the forth reaction solution, mixed evenly, to obtain the fifth reaction solution; and

[0162] 6) Dipotassium glycyrrhizinate and nicotinamide are dissolved by adding water, then added into the fifth reaction solution, and mixed evenly.

Example 6: Effect in Use of the Skincare Product of the Present Invention

[0163] 1. Test samples: the acne-removing skincare product of Example 4 and the acne-removing skincare product of Example 5
2. Subjects: not less than 12-year old in age; female:male≈1:1. [0164] IGA grade 2: 12.25 years old, 15 people; over 25 years old, 15 people. [0165] IGA grade 3: 12.25 years old, 15 people; over 25 years old, 15 people.
3. Test area: whole face
4. Use method: used for 5 days and 28 days; at least twice a day for acne and twice a day for pockmark.

[0166] Treatment in respect to acne: after cleaning the skin, rice-size skincare product was extruded and coated gently around the acne, at least twice daily. For severe acne, number of application can be appropriately increased. Stop using other acne-removing products two weeks prior to the test.

[0167] Treatment in respect to pockmark: after cleaning the skin, rice-size skincare product was extruded and coated gently around the pockmark, twice daily. Stop using other acne-removing products two weeks prior to the test.

5. Test time points: before using the product (Day0), 5 days after using the product (Day5), and 28 days after using the product (Day28)
6. Test parameters:

[0168] After cleaning their faces, the subjects relaxed in the lab (temperature 21±1° C., relative humidity 50±5%) for 20 minutes, expert evaluation and self evaluation on the whole face were carried out before using the product (Day0), 5 days after using the product (Day5), and 28 days after using the product (Day28), respectively. The contents of melanin and heme in skin were assayed by instruments 28 days after using the product.

[0169] 1. Expert Evaluation [0170] A. IGA grading (comprehensive evaluation according to the new/old acne on the whole face) [0171] Grade 0: no inflammatory skin lesions (papule, pustule, nodule, cyst), no non-inflammatory skin lesions (blackhead, whitehead) [0172] Grade 1: rare non-inflammatory skin lesions, one or no small inflammatory skin lesion [0173] Grade 2: slight skin lesions; worse than grade 1; some non-inflammatory skin lesions and a few inflammatory skin lesions (only papules and pustules, no nodules) [0174] Grade 3: moderate skin lesions; worse than grade 2; many non-inflammatory skin lesions, maybe some inflammatory skin lesions, but one or no nodule [0175] Grade 4: severe skin lesions; worse than grade 3; many non-inflammatory and inflammatory skin lesions, a few nodules [0176] B. Skin lesion counting: count the non-inflammatory and inflammatory skin lesions before and after using the products, respectively. [0177] C. Pockmark fading analysis: score the skin evenness, clearness and brightness before and after using the products, respectively. (5-point scale: 1=minor, 5=very obvious) [0178] D. Severity of inflammatory skin lesions (papule, pustule, nodule, cyst) analysis (evaluation according to the old acne on the whole face on photos): [0179] Grade 0: no inflammatory skin lesions (papule, pustule, nodule, cyst) [0180] Grade 1: one or no small inflammatory skin lesion Grade 2: slight skin lesions; worse than grade 1; a few inflammatory skin lesions (only papule and pustule, no nodule) [0181] Grade 3: moderate skin lesions; worse than grade 2; maybe some inflammatory skin lesions, but one or no nodule [0182] Grade 4: severe skin lesions; worse than grade 3; many inflammatory skin lesions, some nodules

[0183] 2. Self-Evaluation of Subjects by Using Questionnaire Survey

[0184] After the use of product for 5 days and 28 days, questionnaire survey was carried out in respect to satisfaction of acne-removing.

[0185] 3. Skin Melanin and Heme Assay

[0186] Skin melanin and heme assay was conducted by using detector Mexameter MX18 to detect the content of melanin and heme in skin.

7. Data Statistics

[0187] Data statistics was performed by SPSS13.0 for windows software. The difference between before and after the test was compared by rank sum test, and the significance level is p≦0.05.

8. Test Results

[0188] 1. IGA Grading

[0189] After the use of test products for 28 days, the severity of skin lesions in IGA2 and IGA3 subjects were observed. The results were shown in Table 3.

TABLE-US-00006 TABLE 3 The changes of IGA grade Grade Grade Grade Grade Grade test samples 0 1 2 3 4 the acne-removing IGA2 Day0 0 0 30  0 0 skincare product of group Day28 5 5 20  0 0 Example 4 IGA3 Day0 0 0 30  0 0 group Day28 5 5 20  0 0 the acne-removing IGA2 Day0 0 0 30  0 0 skincare product of group Day28 5 7 18  0 0 Example 5 IGA3 Day0 0 0  0 30 0 group Day28 2 0 22  6 0

[0190] As shown in Table 3, 28 days after the use of the acne-removing skincare product of Example 4, in IGA2 group, there were 10 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, compared with basal values. The effective improvement rate was 33.3%. In IGA3 group, there were 0 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, and the effective improvement rate was 0%. 28 days after the use of the acne-removing skincare product of Example 5, in IGA2 group, there were 12 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, and the effective improvement rate was 40.0%. In IGA3 group, there were 2 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, and the effective improvement rate was 6.7%.

[0191] 2. Skin Lesion Counting

[0192] Non-inflammatory skin lesions and inflammatory skin lesions were counted before and after the use of test samples. Non-inflammatory skin lesions and inflammatory skin lesions before and after 5 days of use of the product were counted for the subjects in IGA2 and IGA3 groups. The results were shown in Table 4.

TABLE-US-00007 TABLE 4 Skin lesion counting Test samples nodule cyst papule pustule blackhead whitehead the acne-removing IGA2 Day0 0.00 0.00  4.47 0.10 1.97 0.23 skincare product of group Day5 0.00Δ 0.00Δ  3.47Δ 0.13Δ 0.70* 0.20Δ Example 4 Day28 0.00Δ 0.00Δ  2.33 * 0.07Δ 0.63* 0.00Δ IGA3 Day0 0.53 0.10  9.67 0.47 2.30 0.13 group Day5 0.27 * 0.03Δ  6.57 * 0.20Δ 0.90 * 0.07Δ Day28 0.17 * 0.33Δ  5.80 * 0.07 * 1.80Δ 0.03Δ the acne-removing IGA2 Day0 0.00 0.03  5.67 0.20 2.33 0.50 skincare product of group Day5 0.00Δ 0.03Δ  2.10* 0.03Δ 1.43Δ 0.33 Example 5 Day28 0.00Δ 0.03Δ  2.17 * 0.17Δ 0.63 * 0.03 * IGA3 Day0 0.70 0.43 10.40 0.97 2.27 0.70 group Day5 0.33 * 0.23Δ  6.10* 0.33* 1.50Δ 0.83Δ Day28 0.10* 0.17Δ  5.63 * 0.27 * 1.20Δ 0.43Δ Note: rank sum test, * p ≦ 0.05, Δ p > 0.05

[0193] As shown in Table 4, 5 days after the use of the acne-removing skincare product of Example 4, in IGA2 group, blackhead was improved significantly while no obvious change in other symptoms, compared with basal values; in the subjects of IGA3 group, nodule, papule and blackhead were improved significantly while no obvious change in other symptoms. 28 days after the use of the acne-removing skincare product of Example 4, in the subjects of IGA2 group, papule and blackhead were improved significantly while no obvious change in other symptoms, compared with basal values; in the subjects of IGA3 group, nodule, papule and pustule were improved significantly while no obvious change in other symptoms.

[0194] While 5 days after the use of the acne-removing skincare product of Example 5, in IGA2 group, papule was improved significantly while no obvious change in other symptoms, compared with basal values; in the subjects of IGA3 group, nodule, papule and pustule were improved significantly while no obvious change in other symptoms. 28 days after the use of the acne-removing skincare product of Example 5, in the subjects of IGA2 group, papule, blackhead and whitehead were improved significantly while no obvious change in other symptoms, compared with basal values; in the subjects of IGA3 group, nodule, papule and pustule were improved significantly while no obvious change in other symptoms.

[0195] 3. Pockmark Fading Analysis

[0196] Before and after the use of test samples, the skin evenness, clearness and brightness were scored (5-point scale: 1=slight, 5=very obvious). Pockmark fading analysis was performed for the subjects in IGA2 and IGA3 groups before and after 5 days of use of the product. The results were shown in Table 5.

TABLE-US-00008 TABLE 5 Pockmark fading analysis skin skin skin test samples evenness clearness brightness the acne-removing IGA2 Day0 3.40 3.48 3.48 skincare product group Day5 3.47 Δ 3.48 Δ 3.53 Δ of Example 4 Day28 3.48 Δ 3.40 Δ 3.42 Δ IGA3 Day0 2.75 2.63 2.90 group Day5 3.05* 3.03 * 3.17 * Day28 3.03 Δ 3.00 * 3.07 Δ the acne-removing IGA2 Day0 3.33 3.30 3.38 skincare product group Day5 3.50Δ 3.57 * 3.53 Δ of Example 5 Day28 3.38Δ 3.30Δ 3.37Δ IGA3 Day0 2.92 2.93 3.05 group Day5 3.18 * 3.20* 3.15 Δ Day28 3.08Δ 3.12Δ 3.20Δ Note: rank sum test, * p ≦ 0.05, Δ p > 0.05

[0197] As shown in Table 5, 5 days after the use of the acne-removing skincare product of Example 4, in IGA2 group, no significant change in skin evenness, clearness and brightness were observed, compared with basal values, while in IGA3 group, skin evenness, clearness and brightness were all improved significantly. 28 days after the use of the acne-removing skincare product of Example 4, in IGA2 group, no significant change in skin evenness, clearness and brightness were observed, compared with basal values, while in IGA3 group, skin clearness was improved significantly but no obvious change in other parameters.

[0198] 5 days after the use of the acne-removing skincare product of Example 5, in IGA2 group, skin clearness was improved significantly but no significant change in other parameters, compared with basal values; while in IGA3 group, skin evenness and clearness were improved significantly but no significant change in other parameters. 28 days after the use of the acne-removing skincare product of Example 5, no significant change in skin evenness, clearness and brightness were observed in both IGA2 and IGA3 group, compared with basal values.

3. Severity of the Inflammatory Skin Lesions (Papule, Pustule, Nodule, Cyst)

[0199] Before and after 5 days of the use of test samples, severity of the inflammatory skin lesions was observed for the subjects of IGA2 and IGA3 groups. The results were shown in Table 6. (Only the skin lesions which already existed on Day0 were counted; skin lesions appeared after Day0 were not counted.)

TABLE-US-00009 TABLE 6 Changes of inflammatory skin lesions Grade Grade Grade Grade Grade test samples 0 1 2 3 4 the acne-removing IGA2 Day0 0  0 30  0  0 skincare product of group Day5 8  8 14  0  0 Example 4 IGA3 Day0 0  0  0 30  0 group Day5 4  6 19  1  0 the acne-removing IGA2 Day0 0  0 30  0  0 skincare product of group Day5 5 14 11  0  0 Example 5 IGA3 Day0 0  0  0 30  0 group Day5 6  2 20  2  0

[0200] As shown in Table 6, 5 days after the use of the acne-removing skincare product of Example 4, the effective improvement rate of original skin lesions was 53.3% in IGA2 group and 33.3% in IGA3 group, compared with basal values. 5 days after the use of the acne-removing skincare product of Example 5, in IGA2 group, there were 19 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, compared with basal values. The effective improvement rate was 63.3%. In IGA3 group, there were 8 people who had improvement in their skin lesions with grade 0 or grade 1 of severity of skin lesion symptoms or with two grades of improvement in severity, compared with basal values. The effective improvement rate was 26.7%.

4. Self-Evaluation of Subjects

[0201] After the use of product for 5 days and 28 days, questionnaire surveys were carried out in subjects for their satisfaction degree of acne-removing.

4.1 Product Satisfaction Survey after 5 Days of Use of the Product

[0202] 5 days after the use of the product, survey was carried out and the subjects scored their satisfaction degree on the product. Assessment criteria: 7=totally satisfied, 1=not satisfied at all. The results were shown in Table 7.

TABLE-US-00010 TABLE 7 Product satisfaction degree survey the acne-removing the acne-removing skincare product of skincare product of Example 4 Example 5 IGA2 IGA3 IGA2 IGA3 test items group group group group color 5.4 5.0 5.4 5.3 smell 5.4 4.9 5.5 5.4 texture ( viscosity ) 5.8 5.3 5.9 5.5 easy absorption 5.7 5.2 5.7 5.4 instant relieving effect 5.3 5.3 5.5 5.2 mildness 5.8 5.1 5.6 5.6 acne-removing effect 4.6 4.5 5.2 4.5 ( 3 to 5 days ) relieving effect on skin 5.5 5.0 5.9 4.3 discomfort (itch, pain) total satisfaction degree 5.4 5.2 5.5 5.2

[0203] As shown in table 7, in IGA2 group, 5 days after the use of the acne-removing skincare product of Example 4, total satisfaction degree was 5.4; 5 days after the use of the acne-removing skincare product of Example 5, total satisfaction degree was 5.5. In IGA3 group, 5 days after the use of the acne-removing skincare product of Example 4, total satisfaction degree was 5.2; 5 days after the use of the acne-removing skincare product of Example 5, total satisfaction degree was 5.2.

4.2 Acne-Removing Satisfaction Degree Survey 28 Says after the Use of the Product

[0204] 28 days after the use of the product, survey was carried out and the subjects scored their satisfaction degree on acne-removing effect. Assessment criteria: 7=totally satisfied, 1=not satisfied at all. The results were shown in Table 8.

TABLE-US-00011 TABLE 8 acne-removing satisfaction degree survey the acne-removing the acne-removing skincare product of skincare product of Example 4 Example 5 IGA2 IGA3 IGA2 IGA3 test items group group group group pockmark fading effect 5.1 5.0 5.2 5.0 oil control effect 5.4 5.0 5.2 5.3 repeated-occurrence-reducing 5.5 4.9 5.0 5.1 effect total satisfaction degree 5.5 5.1 5.2 5.2

[0205] As shown in table 8, in IGA2 group, 28 days after the use of the acne-removing skincare product of Example 4, total satisfaction degree was 5.5; 28 days after the use of the acne-removing skincare product of Example 5, total satisfaction degree was 5.2. In IGA3 group, 28 days after the use of the acne-removing skincare product of Example 4, total satisfaction degree was 5.2; 28 days after the use of the acne-removing skincare product of Example 5, total satisfaction degree was 5.2.

4.3 Product Property Survey 5 Days after the Use of the Product

[0206] 5 days after use of the product, survey was carried out and the subjects scored the product property. The results were shown in Table 9.

Assessment Criteria:

[0207] paint experience of the acne-removing essence: 1—very difficult to paint, 2—quite difficult to paint, 3—just right, 4—easy to paint, 5—very easy to paint; [0208] texture of the acne-removing essence: 1—too watery, 2—a little bit watery, 3—just right, 4—a little bit sticky, 5—too sticky; [0209] actual onset time of acne-removing: 1—more than one week, 2—within one week, 3—within 5 days, 4—within 3 to 4 days, 5—within 1 to 2 days.

TABLE-US-00012 TABLE 9 Product properties survey product IGA 1 2 3 4 5 test items properties grade score score score score score the acne-removing paint experience IGA2  0.0% 10.0% 20.0% 43.3% 26.7% skincare product of IGA3  0.0%  3.3% 43.3% 36.7% 16.7% Example 4 texture IGA2  0.0%  6.7% 80.0% 13.3%  0.0% IGA3  0.0% 20.0% 60.0% 20.0%  0.0% actual onset time IGA2 26.7% 30.0% 13.3% 20.0% 10.0% of acne-removing IGA3 26.7% 33.3% 16.7% 23.3%  0.0% the acne-removing paint experience IGA2  0.0%  0.0% 36.7% 36.7% 26.7% skincare product of IGA3  0.0%  3.3% 23.3% 56.7% 16.7% example 5 texture IGA2  0.0% 20.0% 70.0%  6.7%  3.3% IGA3  3.3% 30.0% 63.3%  3.3%  0.0% actual onset time IGA2 16.7% 30.0% 16.7% 36.7%  0.0% of acne-removing IGA3 23.3% 26.7% 30.0% 20.0%  0.0%
4.4 Survey of Satisfaction Degree on Adverse Reactions 5 Days after the Use of the Product

[0210] 5 days after use of the product, survey was carried out and the subjects scored their satisfaction degree on the adverse reactions of the product. The results were shown in Table 10. Assessment criteria: 1=very severe, 7=none.

TABLE-US-00013 TABLE 10 survey of satisfaction degree of adverse reactions Complete adverse satisfaction test items reactions IGA 1 2 3 4 5 6 7 % the acne-removing redness IGA2 1 0 0 1 0 1 27 90.0% skincare product IGA3 0 1 0 5 3 3 18 60.0% of Example 4 fever IGA2 0 0 1 2 0 1 26 86.7% IGA3 0 1 1 1 5 2 20 66.7% swelling IGA2 0 1 0 0 0 1 28 93.3% IGA3 0 1 0 2 3 3 21 70.0% itch IGA2 0 1 0 0 1 0 28 93.3% IGA3 0 1 1 4 4 4 16 53.3% pain IGA2 0 1 0 1 0 1 27 90.0% IGA3 0 0 1 4 2 2 21 70.0% the acne-removing redness IGA2 0 0 0 1 2 1 26 86.7% skincare product IGA3 0 1 0 0 0 3 26 86.7% of Example 5 fever IGA2 0 0 0 1 1 1 27 90.0% IGA3 0 0 1 0 0 2 27 90.0% swelling IGA2 0 1 0 0 2 3 24 80.0% IGA3 1 0 0 0 0 2 27 90.0% itch IGA2 0 0 1 1 0 1 27 90.0% IGA3 2 0 0 1 2 2 23 76.7% pain IGA2 0 0 1 0 0 2 27 90.0% IGA3 1 0 0 0 0 2 27 90.0%

5. Assay of Melanin and Heme in Skin

[0211] Skin melanin and heme assay was conducted by using detector Mexameter MX18 to detect the content of melanin and heme in skin. The results were shown in Table 11. Skin melanin value serves as a general standard for judging skin black and white. The whiter the skin color is, the less melanin level. Skin heme serves as a general standard for judging skin red and white. The redder the skin color is, the higher heme level.

TABLE-US-00014 TABLE 11 Skin melanin and heme value test test position control position test items time melanin heme melanin heme IGA2 D0 179 400 156 373 the acne-removing group D28 199 335 165 326 skincare product of p * * Δ * Example 4 IGA3 D0 193 422 165 361 group D28 210 386 180 357 p Δ * Δ Δ the acne-removing IGA2 D0 191 393 163 333 skincare product of group D28 211 342 171 319 Example 5 p * * Δ Δ IGA3 D0 199 441 175 355 group D28 225 375 186 343 p * * * Δ Note: rank sum test, * p ≦ 0.05, Δ p > 0.05

[0212] As shown in Table 11, 28 days after the use of the acne-removing skincare product of Example 4, in IGA2 group, skin melanin value increased significantly and heme value decreased significantly in the test position, compared with basal value; while in control position, melanin value did not change significantly and heme value decreased significantly. In IGA3 group, melanin value did not change significantly and heme value decreased significantly in test position; while in control position, neither melanin nor heme value changed significantly. While 28 days after the use of the acne-removing skincare product of Example 5, in IGA2 group, skin melanin value increased significantly and heme value decreased significantly in test position; while in control position, neither melanin nor heme value changed significantly. In IGA3 group, melanin value changed significantly and heme value decreased significantly in test position; while in control position, melanin value changed significantly and heme value did not change significantly.

6. Skin Adverse Reactions

[0213] During the use of the acne-removing skincare product of Example 4 and the acne-removing skincare product of Example 5, no local skin adverse reactions, such as red spots, papules, wheals, swellings or system skin adverse reactions were observed in the subjects.