IMPROVED METHOD FOR THE PRODUCTION OF HIGH LEVELS OF PUFA IN PLANTS

Abstract

The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).

Claims

1. Raw plant seed oil comprising i. a level of the 18:2 fatty acid fraction in % (w/w) in the triacylglycerol (TAG) composition higher than the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction, ii. a level of the 20:0 fatty acid in % (w/w) in the diacylglyerol fraction higher than the 20:0 fatty acid level in % (w/w) in the triacylglycerol fraction, and/or iii. a level of the 22:1 fatty acid in % (w/w) in the diacylglyerol (DAG) fraction is higher than the SDA level in % (w/w) in the triacylglycerol fraction.

2. (canceled)

3. Method for increasing the delta 6 elongase conversion efficiency in oil crop plant that produces vlcPUFA and expresses a delta-6 elongase, comprising decreasing, compared to a control, in the oil crop plant, plant cell, plant seed, or a part thereof, the activity of one or more PDCT selected from the group consisting of: (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code; and (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.

4. (canceled)

5. (canceled)

6. The method of claim 3, wherein the plant or a part thereof, the plant cell, and/or the plant seed comprises an oil that is characterized by an i. Increased ETA level ii. Reduced ALA level, iii. Reduced GLA level iv. Reduced SDA level, v. The level of the 18:2 fatty acid fraction in % (w/w) in the triacylglycerol (TAG) composition is higher than the 18:2 fatty acid level in % (w/w) in the diacylglyerol (DAG) fraction, vi. The level of the 20:0 fatty acid in % (w/w) in the diacylglyerol fraction is higher than the 20:0 fatty acid level in % (w/w) in the triacylglycerol fraction vii. The level of the 22:1 fatty acid in % (w/w) in the diacylglyerol (DAG) fraction is higher than the 22:1 level in % (w/w) in the triacylglycerol fraction and/or viii. Reduced 18:1 fatty acid level; compared to a control, and, optionally, comprising the further step of isolating the oil from the plant or a part thereof, the plant cell, and/or the plant seed.

7. The method of claim 3, comprising decreasing, compared to a control, in the B. napus plant, plant cell, plant seed, or a part thereof, the activity of one or more PDCT selected from the group consisting of: (a) a PDCT3 having at least 90% sequence identity with SEQ ID NO: 18 or 22 or 24; (b) a PDCT5 having at least 90% sequence identity with SEQ ID NO: 20 or 26 or 28; (c) a PDCT3 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO: 17 or 21 or 23; (d) a PDCT5 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO: 19 or 25 or 27 (e) a PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (f) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 or PDCT5 activity; (g) a PDCT3 encoded by a polynucleotide that differs from SEQ ID NO: 17 or 21 or 23 due to the degeneracy of the genetic code; (h a PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 19 or 25 or 27 due to the degeneracy of the genetic code and (i) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d), (e), (f), (g), or (h) having PDCT3 and/or PDCT5 activity.

8. Method of claim 3 for increasing the d6Elongase conversion efficiency in B. carinata cell, plant or seed that produces ETA, comprising decreasing, compared to a control, in the B. carinata plant, plant cell, plant seed, or a part thereof, the activity of one or more PDCT selected from the group consisting of: (e) a PDCT3 having at least 90% sequence identity with SEQ ID NO: 18 or 22 or 24; (f) a PDCT5 having at least 90% sequence identity with SEQ ID NO: 20 or 26 or 28; (g) a PDCT3 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO: 17 or 21 or 23; (h) a PDCT5 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO: 19 or 25 or 27 (e) a PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (f) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 or PDCT5 activity; (g) a PDCT3 encoded by a polynucleotide that differs from SEQ ID NO: 17 or 21 or 23 due to the degeneracy of the genetic code; (h a PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 19 or 25 or 27 due to the degeneracy of the genetic code and (i) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d), (e), (f), (g), or (h) having PDCT3 and/or PDCT5 activity.

9. Method of claim 3 for increasing the d6Elongase conversion efficiency in B. juncea cell, plant or seed that produces ETA, comprising decreasing, compared to a control, in the B. juncea plant, plant cell, plant seed, or a part thereof, the activity of one or more PDCT selected from the group consisting of: (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code; and (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.

10. (canceled)

11. The method of claim 3, whereby the plant, plant seed or plant cell expresses at least one acyl-CoA dependent desaturase and/or at least one PC-dependent elongase selected from the group consisting of d5-, d5d6-, and d6elongase.

12. The method of claim 3, whereby the plant, plant seed or plant cell expresses at least one delta 6 elongase and/or at least one delta 6-desaturase.

13. (canceled)

14. (canceled)

15. (canceled)

16. The method of claim 3, wherein the plant or plant cell is capable to produce C20 and/or C22 fatty acids, in particular DHA, EPA and DPA.

17. The method of claim 3, wherein the expression of a PDCT is increased, the PDCT is selected from the group consisting of: (a) a PDCT1 having at least 80% sequence identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46; (b) a PDCT1 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45; (c) a PDCT1 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46, or (ii) the full-length complement of (i); (d) a variant of the PDCT1 of SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT1 activity; (e) a PDCT1 encoded by a polynucleotide that differs from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 due to the degeneracy of the genetic code; (f) a fragment of the PDCT1 of (a), (b), (c), (d) or (e) having PDCT1 activity; (g) a PDCT19 having at least 80% sequence identity with SEQ ID NO: 36, 38, and/or 48; (h) a PDCT19 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 35, 37, and/or 47; (i) a PDCT19 encoded by a polynucleotide that hybridizes under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 36, 38, and/or 48, or (ii) the full-length complement of (i); (j) a variant of the PDCT19 of SEQ ID NO: 36, 38, and/or 48 comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT19 activity; (k) a PDCT19 encoded by a polynucleotide that differs from SEQ ID NO: 35, 37, and/or 47 due to the degeneracy of the genetic code; and (I) a fragment of the PDCT19 of (g), (h), (i), (j) or (k) having PDCT19 activity.

18. (canceled)

19. An isolated, a synthetic, or a recombinant polynucleotide: (a) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59, wherein the nucleic acid encodes a polypeptide having PDCT3 and/or 5 activity; (b) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, wherein the polypeptide has PDCT3 and/or 5 activity; (c) a fragment of (a) or (b), wherein the fragment encodes a polypeptide having PDCT3 and/or 5 activity; or (d) a nucleic acid sequence fully complementary to any of (a) to (c), (f) antisense, ribozyme, microRNA polynucleotide comprising a fragment of the PDCT3 and/or PDCT5 polynucleotide of (a), (b), (c), or (d) that binds specifically to the polynucleotide of (a), (b), (c), or (d) and having PDCT3 and/or PDCT5 expression inhibiting activity.

20. The polynucleotide of claim 18 that has a PDCT3 and/or PDCT5 expression reducing or inhibiting activity.

21. (canceled)

22. A polypeptide selected from the group consisting of: (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; (e) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code; and (f) fragments of the PDCT3 and/or PDCT5 of (a), (b), (c), (d) or (e) having PDCT3 and/or PDCT5 activity.

23. Antibody or peptide that specifically binds to the polypeptide of claim 21.

24. The antibody or peptide of claim 23 that reduced or blocks the activity of the polypeptide.

25. An antisenseRNA, ribozyme, or microRNA comprising (1) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; (2) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (3) a fragment of (1) or (2); or (4) a nucleic acid sequence fully complementary to any of (1) to (3), whereby the antisense RNA, ribozyme, or microRNA, when expressed in a plant, plant cell or plant seed that comprises (i) a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59, wherein the nucleic acid encodes a polypeptide having PDCT3 and/or 5 activity; (ii) a nucleic acid sequence encoding a polypeptide having at least 80% sequence identity to SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, wherein the polypeptide has PDCT3 and/or 5 activity; (ii) a fragment of (i) or (ii), wherein the fragment encodes a polypeptide having PDCT3 and/or 5 activity; or expresses a PDCT, wherein the PDCT is selected from the group consisting of: (a) a PDCT3 and/or PDCT5 having at least 90% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60; (b) a PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 90% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; (c) PDCT3 and/or PDCT5 encoded by one or more polynucleotides that hybridize under high stringency conditions with (i) a polynucleotide that encodes the amino acid sequence of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, or (ii) the full-length complement of (i); (d) variants of the PDCT3 and/or PDCT5 of SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60, comprising a substitution, deletion, and/or insertion at one or more positions and having PDCT3 and/or PDCT5 activity; and (e) a PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60 due to the degeneracy of the genetic code, and reduces the expression of said nucleic acid sequence or said PDCT3 and/or 5.

26. Antisense RNA, ribozyme, microRNA polynucleotide comprising a fragment of the PDCT3 or PDCT5 polynucleotide of claim 24 that binds specifically to the polynucleotide.

27. A nucleic acid construct comprising a polynucleotide of claim 20, operably linked to one or more heterologous control sequences that directs the expression of the protein of interest in a cell.

28. A antisenseRNA, ribozyme, or microRNA that inhibits the expression of a gene comprising the sequence of the polynucleotide of claim 20.

29. A antisenseRNA, ribozyme, or microRNA of claim 27 that comprises a fragment of SEQ ID NO. 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59.

30. A vector or expression construct comprising the polynucleotide of claim 20.

31. A host cell which is transformed with a polynucleotide of claim 20.

32. The host cell of claim 31, wherein said host cell is selected from the group consisting of Agrobacterium, yeast, bacterial, algae or plant cell.

33. (canceled)

34. (canceled)

35. (canceled)

36. (canceled)

37. A plant, or part thereof, a plant seed, a plant cell, or a plant oil comprising C18 to C22 fatty acids, wherein the ALA+LA level is less than C18 to C22 fatty acids having an increased PDCT1 and/or PDCT19 expression or acitivity.

38. (canceled)

39. (canceled)

40. (canceled)

41. (canceled)

42. (canceled)

43. (canceled)

44. (canceled)

45. (canceled)

Description

FIGURES

[0485] FIG. 1 Alignment of PDCT protein sequences [0486] Legend: At: Arabidopsisthaliana, Bn: Brassicanapus, Bc: Brassicacarinata, Cs: Camelinasativa, Gm: Glycine max, Lu: Linumusitatissimum, Rc: Ricinuscommunis, Ta: Triticumaestivum, Zm: Zeamays. [0487] *activity demonstrated in other studies [0488] ** proteins selected based on homology in BLAST searches of NCBI databases, activity not demonstrated [0489] Color setup: Non-similar, weakly similar: dark grey, conserved: light grey, blocks of similar: medium grey, identical: white

[0490] FIG. 2 Alignment of N-terminal region of C. sativa sequences. All differences in the C. satvia proteins are within this region

[0491] Color setup: Non-similar, weakly similar: dark grey, conserved: light grey, blocks of similar: medium grey, identical: white

[0492] FIG. 3 Phylogenetic tree based on PDCT protein sequences. [0493] Legend: At: Arabidopsisthaliana, Bn: Brassicanapus, Bc: Brassicacarinata, Cs: Camelinasativa, Gm: Glycine max, Lu: Linumusitatissimum, Rc: Ricinuscommunis, Ta: Triticumaestivum, Zm: Zeamays. [0494] *activity demonstrated in other studies [0495] ** proteins selected based on homology in BLAST searches of NCBI databases, activity not demonstrated

[0496] FIG. 4. Pathway and genes in fatty acid synthesis pathway in transgenic Arabidopsis plants.

[0497] FIG. 5. Action of PDCT (Modified from Lu et al., 2009)

[0498] FIG. 7 decribes the formulas to calculate pathway step conversion efficiencies. S: substrate of pathway step.

[0499] P: product of pathway step. Product was always the sum of the immediate product of the conversion at this pathway step, and all downstream products that passed this pathway step in order to be formed. E.g. DHA (22:6n-3 does possess a double bond that was a result of the delta-12-desaturation of oleic acid (18:1n-9) to linoleic acid (18:2n-6).

[0500] FIG. 8: Needle Matrix of PCDT sequences

[0501] FIG. 9: Conversion efficiencies of desaturases

EXAMPLES

Example 1 Materials and Methods

Cloning of Genes

[0502] RNA from young root tissue of B.napus, B.carinata and C.sativa was reversed transcribed using Superscript III. Primers for cloning cDNAs were based on genomic sequence information from NCBI sequence databases (https://www.ncbi.nlm.nih.gov/) and naming of genes followed the information in these databases. The proofreading enzyme Phusion was used to clone cDNAs, which were transformed into pYes 2.1 prior to sequencing. Seven PDCT like genes were cloned from B.napus, originating from chromosome 1A, 1C, 2C, 3A, 3C, 5A and 5C. Seven genes were cloned from B.carinata, originating from chromosomes 1B, 1C, 2B, 3B, 3C, 5B and 5C. Three genes were cloned from C.sativa, originating from chromosomes 1, 15 and 19. Sequences of cDNAs and translation products are given in Table 1.

Example 2 Sequence Analysis

[0503] All clones were sequenced prior to transformation. The protein alignment and phylogenetic tree were constructed using the software program Vector NTI.

Example 3 Construction of Transformation Vectors and Arabidopsis Transformation

[0504] Because the C genome genes from B.carinata and B.napus were identical or nearly identical, only C subgenome derived PDCT genes from B.carinata were used in further experiments. PDCT genes were cloned into the pUC-19 Napin-B vector to add the Napin promotor and OSC terminator, as described in Wu et al (2005). The genes including promoters and terminators were removed by restriction enzyme digestion and ligated to pUC19-ABC carrying the Thraustocytrium sp. delta 6 elongase (Sequence ID: KH273553.1) and the P. irregulare delta 6 desaturase (Sequence ID: AF419296.1). The three genes were removed from the vector by restriction enzyme digestion and ligated into the plant binary vector pSUN2-ASC. All vectors were analyzed by restriction digestion before transformation. Controls included an empty vector and a vector containing only the P. irregulare D6 desaturase and the PSE (tc) elongase . The Arabidopsis rod1 (At3g15820) mutant line (Lu et al. 2009), kindly provided by Chaofu Lu, was used as the Arabidopsis host plant. This mutant has a G to A mutation resulting in a premature stop codon in the phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) enzyme encoded by the Arabidopsis ROD1 gene (Lu et al. 2009). Four plants were tested by sequencing, which indicated all were homozygous for the relevant mutation, and seed was collected from these plants and used for transformation. Plant binary vectors were transformed into Agrobacterium tumefaciens strain GV3101-pMP90. The host plant was grown until the bolting stage and transformed using the floral dip method (Clough and Bent, 1998). Essentially, Agrobacterium tumefaciens carrying each vector was grown to mid-log stage, spun down and suspended to an OD600 of 0.8 in 5% sucrose solution containing 0.05% Silwet L-77, and plants were immersed in this solution for 2-3 minutes with gentle agitation. After maturity, seeds were sterilized and germinated on ½X MS selective medium containing 50 mg/L kanamycin for selection of transgenic plants. Positive plants were transplanted into soil and grown to maturity.

Example 4: GC Analysis

[0505] Twenty T2 seeds from positive T1 plants were used to extract fatty acids. Seeds were placed in a clean glass tube, 2 mL of 3 M methanolic HCL was added to each tube, and capped tubes were incubated at 80° C. for 4 hours. After incubation, samples were cooled to room temperature, 1 mL of 0.9% NaCl and 2 mL of hexane was then added to each sample and vortexed. Samples were then centrifuged and the hexane (top) layer was removed and added to clean glass tubes. Samples were evaporated under nitrogen until dry. 80 .Math.L of hexane was added to the tubes and vortexed briefly to resuspend the fatty acids. The solution was then moved to a collection vial containing a GC insert, and GC analysis was performed (Table 2).

[0506] The segregation of the transgene was tested by germinating 50-100 seeds on selective media, and testing the fit to a 3:1 hypothesis (Table 3). Seedling progeny of transgenic plants that segregated with a 3:1 ratio (consistent with expression of construct at a single locus) were used for further analysis. GC analysis of 20 seeds from 3-5 lines for each gene was conducted as described above, and fatty acid distribution was determined (Table 4).

Example 5 Results

[0507] The amino acid sequences of the 19 PDCT genes cloned in this study fell in 5 distinct groups (FIGS. 1, 2 and 3). These groups consisted of the chromosome 1-derived sequences of B.napus and B. carinata, the chromosome 2 sequences of B.napus and B.carinata, the chromosome 3 sequences of B.carinata and B.napus, the chromosome 5 genes of B.napus and B.carinata and the three C.sativa sequences (FIG. 2). The amino acid translations of the C-subgenome derived genes of B.carinata and B.napus were identical or nearly identical, although there were differences in the cDNA sequences (FIG. 1, Table 1). Most of the differences in amino acid sequences occurred in the N-terminal region of the translation products, while blocks of conserved amino acids were found throughout the middle and C-terminal regions (FIG. 1). The Group 1 sequences were about 42 amino acids shorter than the other sequences in this area. The differences among the three C. sativa sequences occurred within the first 60 amino acids (FIG. 1, FIG. 2).

[0508] The four subgenome A PDCT genes from Brassicanapus, the four subgenome B and four subgenome C genes from Brassicacarinata, and all three PDCT genes from Camelinasativa were co-expressed in the Arabidopsis rod1 mutant with the Δ6-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium. The Arabidopsis rod1 mutant and a wild-type Arabidopsis line (with an active endogenous PDCT gene) were also transformed with the Δ6-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium, and untransformed wild-type and ROD mutant lines were used for comparison.

[0509] Expression of the Δ6-desaturase and Δ6-elonagase will result in the production of the heterologous fatty acids γ-linolenic acid (GLA ; 18:2 Δ11, 14), stearidonic acid (SDA ; 18:3 Δ6 9, 12, 15), di-homo γ-linolenic acid (DGLA; 20:3 Δ8, 11, 14 ) and eicosatetraenoic acid (ETA; 20:4 Δ8.11, 14,17) in Arabidopsis seeds, as shown in FIG. 4. An active PDCT gene will lead to a decrease in the level of OA (18:1 Δ9) and an increase in the level(s) of LA (18:2Δ6, 9), ALA (18:3Δ6, 9, 15) and/or GLA, as shown in FIG. 5.

[0510] The presence of a mutation in the ROD1 gene of Arabidopsis has been shown to increase the percent of 18:1 in seed oil (Lu et al., 2009). The percentage of 18:1 in the untransformed rod1 mutant used in this study averaged 30.42%, while seed oil of the untransformed wild-type line contained 15.334% 18:1. Seed oil from Arabidopsis lines carrying group 1 and group 2 chromosome-derived PDCT genes had average 18:1 levels ranging from 25.72-31.12% (Table 2). This was comparable to the level in the ROD mutant lines transformed with only the Δ6-desaturase and Δ6-elonagase (average 30.732%). However, the levels in seeds carrying the subgenome 3A, 3B and 3C derived genes ranged from 14.959-15.871%. Levels in seeds carrying chromosome 5 derived PDCT genes ranged from 11.994-16.696%, and those in seeds carrying the C. sativa genes ranged from 13.288-14.050%. Thus, while the Brassicanapus chromosome 3 and chromosome 5 derived genes, and the three C.sativa genes are able to compensate for the mutation in the Arabidopsis PDCT gene, the chromosome 1 and 2 derived genes appear to have little or no effect on 18:1 levels. This suggests that the chromosome 1 and 2 derived genes may have a different function and/or act on different substrates than the Arabidopsis PDCT gene.

[0511] Alignment of PDCT-like translation products from a range of species including Triticumaestivum, Arabidopsisthaliana, Zeamays, Ricinuscommunis, Glycine max, and Linumusitatissimum indicated that substitutions of highly conserved amino acids occurred throughout the B.napus chromosome 1 and chromosome 2 derived proteins. Using numbering based on the Arabidpsis ROD1 sequence as shown in the alignment in FIG. 1, Brassica napus chromosome-1 derived enzymes showed the following changes in conserved regions: position 102: M to T, between 104-105: insertion of E, and 225: H to Q. In addition to these changes in conserved regions, various differences occurred in the less conserved N-terminal region of the protein.

[0512] In the case of chromosome 2B and 2C derived proteins from Brassicacarinata and Brassicanapus respectively, a larger number of substitutions in conserved regions were detected. Using amino acid residue numbering based on the Arabidopsis ROD1 sequence, the following substitutions were detected 98: V/L to F, 101 F to V, 102 M to V, 106: Y to S, 141: L/V to G, 149-150: FV to LG, 158: L/V to A, 176: M to V, 186: S/A to C, 192: P to S, 211: L to Y, and 230: M/V to T. Notably, this threonine substitution at position 230 also occurred in most of the chromosome 1 group proteins, as did the M to T substitution at position 106.

[0513] In the untransformed Arabidopsis wild-type lines the decrease in 18:1 is compensated for by an increase in 18:2 compared to rod1 mutant plants (27. 545% in wild-type versus 14.323% in ROD mutant; Table 2) although a slight increase in ALA also occurs (16.066 versus 14.323%). Transgenic lines carrying the elongase and desaturase genes plus chromosome - 1 or 2 PDCT genes had LA levels of 8.314-12.165%, while lines carrying chromosome 3 and 5 derived PDCT genes had levels of 18.149-20.142%. The lines carrying the C.sativa genes had 18:2 levels of 11.324% (Chromosome 1 derived PDCT), 19.912% (C15) and 8.635% (C19). ALA levels were also comparatively low in lines carrying the C.sativa C1 (7.771%) and C19 (7.656%) genes, whereas lines containing the C15 genes had the highest average ALA content (14.826%). However, in lines carrying the Δ6-desaturase and the Δ6-elonagase along with the PDCT gene, the additional 18:2 produced in the presence of the PDCT gene may be used not only to produce ALA, but may also be used in the synthesis of GLA, DGLA, SDA and ETA (FIG. 4). The total levels of these fatty acids were highest in lines carrying the C1 (25.225%) and C19 (24.379%) PDCT genes, and these two lines also had the highest levels of GLA plus HGLA (22.183% and 21.094% respectively). The fatty acid profile of lines carrying the C.sativa C15 gene bore more of a resemblance to the group 5 and group 3 chromosomes, in that the total ALA plus SDA plus ETA (16%) was considerably higher than the total GLA plus HGLA (8.767%). Only in the C1 and C19 lines were total levels of GLA plus HGLA higher than total levels of ALA plus SDA plus ETA (Table 2). Thus, not only do the various PDCTs show differences in overall efficiency, but there also appears to be different substrate preferences among the genes. The Camelina sativa C1 and C19 proteins differed from the C15 protein in only a limited number of amino acids in the N-terminal region of the protein (FIG. 2). Position 3 was valine in C15 and alanine in C1 and C19. Position 4 was alanine in C15, whereas the similar amino acid residues serine and threonine were at position 4 in C1 and C19 respectively. A conserved histidine at position 20 in C1 and C19 was replaced by asparagine in C15, proline-valine residues at positions 35 to 36 in C1 and C19 were replaced with arginine-isoleucine in C15, and a threonine at position 41 was replaced with lysine in C15. Finally, C15 had an insertion of an amino acid (glycine) at position 63. These differences indicated the importance of the N-terminal region of the PDCT enzyme in determining enzyme activity.

[0514] Potentially, inactivation of one or more Camelina sativa PDCT enzyme may modulate PDCT activity levels, and might also be beneficial in increasing the levels of specific fatty acids, or in pushing fatty acids towards the ω3 or ω6 pathway. Since B. napus and B. carinata each have four active PDCT genes, it should be possible to achieve a range in PDCT activity levels by combining active and inactive genes. Avoiding rapid transfer onto DAG may allow more efficient transfer to the acyl-CoA pool by the reverse reaction of plant LPCAT enzymes. The reverse reaction of LPCAT has been shown to play an important role in editing PC in plants, and plant LPCATs also show fatty acid selectivity (Lager et al., 2013) This may be of particular interest for the production of VLC-PUFAs, where rapid movement of fatty acids to the DAG pool and subsequently to TAG may not be desirable.

[0515] To ensure the differences in activities among the transgenic lines did not reflect differences in copy numbers of PDCT genes, the segregation ratio of T2 plants was checked (Table 3), and T3 seed from lines that fit a 3:1 segregation ratio was used for GC analysis. Results closely resembled those from the T2 generation (Table 4). 18:1 levels in lines carrying chromosome group 1 or 2 derived PDCT genes ranged from 31.26-31.41%, while levels in group 3 and 5 lines ranged from 12.17-14.59%. Levels in lines carrying the C. sativa genes ranged from 12.89 to 14.60%. LA levels in lines carrying group 1 and 2 chromosome genes ranged from 6.58-10.06%, while levels in the group of lines carrying chromosome 3 or 5 derived genes ranged from 15.58-23.54%. Levels in lines carrying C1, C15 and C19 PDCT genes were 11.53, 21.49 and 7.50%, respectively. Again, the low level of LA in C1 and C19 lines was due to the very high levels of GLA plus DGLA in these lines (20.85% in C1 and 23.11% in C19).

Example 6 Average Fatty Acid Composition (%) in Different Lipid Classes from Immature Seeds

[0516] Thin-layer chromatography (TLC) analysis was performed on immature siliques (from plants homozygous for the desaturase and elongase transgenes) to measure the fatty acid profile in different lipid pools, namely, phosphatyidylcholine (PC), diacylglycerol (DAG), and triacylglycerol (TAG). Briefly, total lipids were extracted from immature siliques by rapid freezing and grinding of green siliques, followed by transferring approximately 500 mg of ground sample into a centrifuge tube with 3 ml of chloroform: methanol: formic acid (10:10:1, v/v/v) and storing overnight at -20° C. After centrifugation, the supernatant was collected, and the pellet was re-extracted with 1.1 ml chloroform: methanol: water (5:5:1, v/v/v). The extractions were combined and washed with 1.5 ml mL 0.2 M H3PO4/ 1M KCI. Lipids in the chloroform phase were dried down, and re-dissolved in 0.2 ml of chloroform. After pre-running and drying the TLC plate, samples were run in hexane/diethyl ether/acetic acid (70:30:1). TAG and DAG were isolated and directly methylated with 3 M methanolic HCL. Polar lipids were collected from the plate, extracted and resuspended in chloroform, then re-run in chloroform/methanol/acetic acid/water (60:30:3:1) to separate PC.

[0517] Bands were visualized by spraying with primulin solution and exposing to UV light. The appropriate silica bands were scraped from the TLC plate, and treated with 2 mL 3 M methanolic HCL at 80° C., then analyzed by GC. All fatty acid data are presented as % relative and are shown in Table 7.

[0518] The data in Table 7 can be used to understand how the various PDCT genes influence the trafficking of fatty acids between different lipid pools. Table 7 shows the average fatty acid composition (%) in different lipid classes from immature seeds of Arabidopsis transformed with D6(Pi) desaturase+ Tc D6Elongase. 3C and 5C refers to data from plants with a knock out of PDCT3 and PDCT5, respectively.

[0519] The Arabidopsis rod1 mutant (CK mutant) and a wild-type Arabidopsis line (CK WT) (with an active endogenous PDCT gene) were also transformed with the Δ6-desaturase from Pythium irregulare and the Δ6-elonagase from Thraustochytrium, and untransformed wild-type (WT) and ROD mutant lines (Rod mut) were used for comparison.

TABLE-US-00005 Sequenzes: PDCT1 Polypeptide: SEQ ID No.: 2, 4, 6, 8, 10, 12, 14, 16, 40, 42, 44, and/or 46 PDCT1 Polynucleotide: SEQ ID No.: 1, 3, 5, 7, 9, 11, 13, 15, 39, 41, 43, and/or 45 PDCT19 Polypeptide: SEQ ID No.: 36, 38, and/or 48 PDCT19 Polynucleotide: SEQ ID No.: 35, 37, and/or 47 PDCT3/5 Polypeptide: SEQ ID No.: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60 PDCT19 Polynucleotide: SEQ ID No.: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, and/or 57 Candiates of the PDCT1 that shall have the same activity as PDCT1: GmROD1-1 63 64 PDCT1 candiate GmROD1-2 65 66 PDCT1 candiate RcPDCT 67 68 PDCT1 candiate RcROD1_SEQIDNO9 69 70 PDCT1 candiate LuPDCT1 71 72 PDCT1 candiate LuPDCT2 73 74 PDCT1 candiate

TABLE-US-00006 Average fatty acid composition (%) in seeds of PDCT+D6(Pi) desaturase+ Tc D6Elongase transgenic T2 Arabidopsis 16:0 18:0 18:1 18:2 GLA 18:3 20:1 HGLA SDA ETA Total GLA HGLA SDA ETA Total ALA SDA ETA Total GLA HGLA Napus 1A 9.063 3.290 26.438 12.165 2.205 9.916 15.452 5.634 0.231 0.995 9.065 11.142 7.839 Carinat a 1C 8.254 3.398 29.693 11.760 2.402 10.656 17.882 1.950 0.689 0.369 5.410 11.714 4.352 Carinat a 2B 7.950 3.203 30.947 11.418 3.083 11.154 18.672 1.639 0.742 0.253 5.717 12.149 4.722 Napus 2C 8.045 3.239 29.547 11.834 3.903 10.586 17.472 1.703 0.898 0.237 6.74 1 11.721 5.606 Napus 3A 7.915 3.004 15.871 18.613 7.984 12.877 17.168 1.827 1.226 0.090 11.127 14.193 9.811 Carinat a 3B 7.756 3.027 15.287 18.974 7.977 13.180 17.220 1.777 1.282 0.053 11.089 14.515 9.755 Carinat a 3C 7.846 3.495 14.959 17.639 8.662 13.744 18.638 2.096 1.374 0.215 12.347 15.333 10.758 Napus 5A 7.606 3.286 16.696 18.657 6.467 14.627 18.890 1.192 1.092 0.065 8.81 6 15.784 7.659 Carinat a 5B 8.031 3.244 16.696 18.149 9.193 12.762 16.790 2.481 1.493 0.155 13.322 14.410 11.674 Carinat a 5C 8.429 2.905 15.025 20.812 9.901 11.717 15.036 2.453 1.365 0.102 13.821 13.184 12.354 C1(806 66) 9.126 3.440 13.288 11.324 14.380 7.771 15.141 7.803 2.063 0.980 25.225 10.813 22.183 C15(45 897) 8.196 3.489 14.367 19.912 7.366 14.8 26 18.397 1.401 1.1 58 0.016 9.941 16.000 8.767 C19(65 416) 7.830 3.454 14.050 8.635 14.658 7.656 15.746 6.436 2.4 40 0.844 24.379 10.940 21.094 CK mutant 8.936 3.290 30.732 11.866 3.172 11.105 17.449 1.905 0.602 0.198 5.877 11.905 5.077 CK WT 7.684 3.345 12.7 54 22.068 7.527 13.765 18.000 1.906 0.968 0.143 10.544 14.876 9.433 WT 7.335 3.284 15.334 27.545 0.000 16.066 18.071 0.000 0.000 0.000 0.000 16.066 0.000 ROD mut 7.619 3.123 30.420 14.3 32 0.000 15.158 19.276 0.0 00 0.0 00 0.0 00 0.000 15.158 0.000 CK mutant: PDCT mutant with D6(Pi) desaturase+ Tc D6Elongase CK WT: WT Arabidopsis with D6(Pi) desaturase+ Tc D6Elongase WT : Untransformed wild-type Arabidopsis ROD mut: Untransformed Arabidopsis ROD mutant Complete data in Appendix 1.

TABLE-US-00007 Segregation ratios of T.sub.2 generation to test goodness of fit to 3:1 ratio Group Plant # Resistant plant Susceptible plant Hypothesis Ratio p value Accept hypothesis B.napus 1A 2 50 0 63:1 0.312 Accept 4 12 9 3:1 0.04 No 5 71 19 3:1 0.46 Accept 6 61 11 3:1 0.06 Accept 7 20 45 3:1 0.249 Accept 8 40 13 3:1 1 Accept 9 41 26 3:1 0.012 No 10 38 16 3:1 0.34 Accept 11 40 16 3:1 0.537 Accept 12 65 20 3:1 0.801 Accept 13 67 18 3:1 0.451 Accept 14 32 15 3:1 0.316 Accept 15 50 9 3:1 0.073 Accept 16 103 23 3:1 0.1 Accept 17 54 19 3:1 0.786 Accept 18 35 64 1:3 0.021 No 19 54 18 3:1 1 Accept 20 74 14 3:1 0.049 No 21 22 8 3:1 1 Accept 22 83 23 3:1 0.498 Accept 23 52 17 3:1 1 Accept 24 73 16 3:1 0.14 Accept Group Plant # Resistan t plant Susceptible plant Hypothesis Ratio p value Accept hypothesis or not B. carinata 2B 1 72 20 3:1 0.47 Accept 2 59 19 3:1 1 Accept 3 73 23 3:1 0.814 Accept 4 45 15 3:1 1 Accept 5 99 5 15:1 0.674 Accept 6 75 9 15:1 0.065 Accept 7 103 11 15:1 0.119 Accept 8 107 16 3:1 0.001 No 9 98 12 15:1 0.051 Accept 10 119 5 15:1 0.273 Accept 12 50 0 63:1 0.312 Accept 13 136 16 15:1 0.016 No 14 113 19 3:1 0.005 No 15 142 11 15:1 0.744 Accept 16 50 5 15:1 0.235 Accept 17 84 11 15:1 0.035 No 18 88 29 3:1 1 Accept 19 107 9 15:1 0.435 Accept 20 105 10 15:1 0.242 Accept 21 101 25 3:1 0.215 Accept 22 76 3 15:1 0.355 Accept 23 65 16 3:1 0.302 Accept 24 51 21 3:1 0.414 Accept 25 51 16 3:1 0.779 Accept B. napus 2C 1 95 20 3:1 0.053 Accept 2 55 17 3:1 0.785 Accept 3 50 2 15:1 0.552 Accept 4 120 12 15:1 0.145 Accept 5 130 16 15:1 0.016 No 6 160 14 15:1 0.35 Accept 7 103 10 15:1 0.242 Accept 8 60 19 3:1 0.796 Accept 9 74 26 3:1 0.817 Accept 10 58 25 3:1 0.313 Accept 11 118 12 15:1 0.144 Accept 12 98 2 63:1 1 Accept 13 42 24 3:1 0.027 No 14 71 1 63:1 1 Accept 15 75 25 3:1 1 Accept 16 38 29 3:1 0.001 No 17 125 21 3:1 0.004 No 18 143 35 3:1 0.118 Accept 19 107 2 63:1 1 Accept 20 81 23 3:1 0.497 Accept 21 60 1 63:1 1 Accept 22 92 1 63:1 1 Accept Group Plant # Resistan t plant Susceptible plant Hypothesis Ratio p value Accept hypothesis B. napus 3A 1 67 12 3:1 0.038 No 2 125 39 3:1 0.718 Accept 3 29 26 3:1 0 No 4 92 21 3:1 0.127 Accept 5 67 20 3:1 0.622 Accept 6 43 19 3:1 0.342 Accept 7 55 26 3:1 0.236 Accept 8 70 9 15:1 0.065 Accept 9 60 7 15:1 0.122 Accept 10 63 5 15:1 0.606 Accept 11 60 18 3:1 0.792 Accept 12 68 22 3:1 1 Accept 13 56 29 3:1 0.044 No 14 69 22 3:1 0.809 Accept 15 70 2 63:1 0.314 Accept 16 41 13 3:1 1 Accept 17 53 3 15:1 1 Accept 18 47 16 3:1 1 Accept 19 77 13 3:1 0.027 No 20 78 6 15:1 0.645 Accept 21 90 15 3:1 0.013 No 22 47 11 3:1 0.357 Accept 23 35 11 3:1 1 Accept 24 61 20 3:1 1 Accept B. carinata 3B 3B-1 76 7 15:1 0.3562 Accept 3B-2 56 23 3:1 0.4376 Accept 3B-3 24 28 3:1 <0.000 1 Reject 3B-4 58 15 3:1 0.415 Accept 3B-5 27 45 3:1 <0.000 1 Reject 3B-6 142 37 3:1 0.168 Accept 3B-7 85 31 3:1 0.668 Accept 3B-8 87 21 3:1 0.182 Accept 3B-9 75 24 3:1 0.817 Accept 3B-10 97 11 15:1 0.118 Accept 3B-11 52 13 3:1 0.388 Accept 3B-12 43 18 3:1 0.372 Accept 3B-13 75 29 3:1 0.497 Accept 3B-14 63 3 15:1 0.606 Accept 3B-15 42 16 3:1 0.539 Accept 3B-16 70 4 15:1 0.643 Accept 3B-17 68 2 63:1 0.314 Accept 3B-18 56 23 3:1 0.438 Accept 3B-19 59 5 15:1 0.606 Accept 3B-20 71 2 63:1 0.314 Accept 3B-21 56 2 63:1 0.313 Accept 3B-22 58 22 3:1 0.606 Accept 3B-23 59 19 3:1 1 Accept 3B-24 65 30 3:1 0.157 Accept B.carinata 3C 1 128 2 63:1 1 Accept 2 96 17 3:1 0.017 No 5 78 24 3:1 0.818 Accept 6 76 8 15:1 0.167 Accept 7 50 5 15:1 0.235 Accept 8 91 15 3:1 0.013 No 9 75 10 15:1 0.021 No 10 95 17 3:1 0.016 No 11 97 13 15:1 0.019 No 12 38 12 3:1 1 Accept 13 80 10 15:1 0.091 Accept 14 42 6 15:1 0.074 Accept 15 77 17 3:1 0.15 Accept 16 70 5 15:1 1 Accept 17 120 1 63:1 0.476 Accept 18 79 24 3:1 0.65 Accept 19 61 15 3:1 0.289 Accept 20 94 20 3:1 0.082 Accept 21 59 13 3:1 0.174 Accept 22 109 15 15:1 0.011 No 23 49 19 3:1 0.575 Accept 25 53 17 3:1 1 Accept 34 65 22 3:1 1 Accept 39 77 23 3:1 0.644 Accept 24 58 1 63:1 1 Accept Group Plant # Resistan t plant Susceptible plant Hypothesis Ratio p value Accept hypothesis B.napus 5A 5A-1 32 17 3:1 0.097 Accept 5A-3 53 17 3:1 1 Accept 5A-4 49 14 3:1 0.563 Accept 5A-5 50 21 3:1 0.413 Accept 5A-6 36 13 3:1 0.74 Accept 5A-8 70 6 15:1 0.644 Accept 5A-9 35 11 3:1 1 Accept 5A-10 32 15 3:1 0.316 Accept 5A-11 47 7 15:1 0.017 Reject 5A-12 71 1 63:1 1 Accept 5A-13 52 15 3:1 0.574 Accept 5A-14 45 17 3:1 0.553 Accept 5A-15 61 28 3:1 0.14 Accept 5A-16 61 24 3:1 0.451 Accept 5A-17 78 25 3:1 0.821 Accept 5A-18 56 24 3:1 0.302 Accept 5A-19 46 14 3:1 0.766 Accept 5A-20 60 19 3:1 0.796 Accept 5A-21 86 14 3:1 0.011 Reject 5A-23 54 9 15:1 0.01 Reject 5A-25 48 17 3:1 0.773 Accept 5A-26 53 18 3:1 1 Accept 5A-1 32 17 3:1 0.097 Accept 5A-3 53 17 3:1 1 Accept 5A-4 49 14 3:1 0.563 Accept 5A-5 50 21 3:1 0.413 Accept B. carinata 5B 5B-1 54 3 15:1 0.6041 Accept 5B-2 49 15 3:1 0.7728 Accept 5B-3 50 12 3:1 0.3737 Accept 5B-4 59 20 3:1 1 Accept 5B-5 76 29 3:1 0.4976 Accept 5B-6 58 12 3:1 0.1634 Accept 5B-7 68 21 3:1 0.806 Accept 5B-8 67 22 3:1 1 Accept 5B-9 74 18 3:1 0.229 Accept 5B-10 112 26 3:1 0.114 Accept 5B-11 48 20 3:1 0.401 Accept 5B-12 53 21 3:1 0.416 Accept 5B-13 57 24 3:1 0.303 Accept 5B-14 63 16 3:1 0.301 Accept 5B-15 107 9 15:1 0.435 Accept 5B-16 99 32 3:1 0.84 Accept 5B-17 56 14 3:1 0.403 Accept 5B-18 56 19 3:1 1 Accept 5B-19 42 23 3:1 0.044 Reject 5B-20 125 7 15:1 0.715 Accept 5B-21 26 29 3:1 <0.000 1 Reject 5B-22 33 11 3:1 1 Accept 5B-23 51 19 3:1 0.784 Accept B. carinata 5C 5C-18 118 42 3:1 0.715 Yes 5C-11 76 26 3:1 0.8179 Yes 5C-15 114 101 3:1 0.0001 No 5C-12 70 16 3:1 0.2095 Yes 5C-2 52 17 3:1 1 Yes 5C-10 79 3 15:1 0.356 Yes 5C-26 88 13 3:1 0.0057 No 5C-20 59 23 3:1 0.4404 Yes 5C-25 60 16 3:1 0.4268 Yes 5C-5 66 14 3:1 0.1213 Yes 5C-19 45 6 3:1 0.0245 No 5C-6 95 3 15:1 0.2062 Yes 5C-16 93 94 3:1 0.0001 No 5C-9 112 7 15:1 1 Yes 5C-17 116 37 3:1 0.8516 Yes 5C-8 156 58 3:1 0.529 Yes 5C-13 72 43 3:1 0.0026 No 5C-1 72 27 3:1 0.4817 Yes 5C-7 140 124 3:1 0.0001 No 5C-14 41 24 3:1 0.0213 No 5C-3 64 33 3:1 0.0342 No Group Plant # Resistan t Susceptible Hypothesis Ratio p value Accept hypothesis C. sativa C1 80666 -15 54 8 3:1 0.0684 Yes 80666 -20 50 12 3:1 0.3737 Yes 80666 -17 52 20 3:1 0.5862 Yes 80666 -13 48 18 3:1 0.5657 Yes 80666 -1 24 29 3:1 0.0001 No 80666 -3 39 32 3:1 0.0001 No 80666 -16 45 17 3:1 0.5531 Yes 80666 -19 55 18 3:1 1 Yes C. sativa C15 45897 -16 68 17 3:1 0.3144 Yes 45897 -5 60 20 3:1 1 Yes 45897 -18 63 18 3:1 0.6063 Yes 45897 -8 82 6 15:1 0.6452 Yes 45897 -14 51 16 3:1 0.7789 Yes 45897 -1 53 8 3:1 0.0374 No 45897 -15 66 4 15:1 1 Yes 45897 -9 55 17 3:1 0.7855 Yes 45897 -13 81 19 3:1 0.1659 Yes 45897 -12 58 20 3:1 0.7919 Yes 45897 -11 58 30 3:1 0.0489 No 45897 -10 59 15 3:1 0.4163 Yes 45897 -6 58 17 3:1 0.5954 Yes 45897 -7 63 21 3:1 1 Yes 45897 -17 53 16 3:1 0.78 Yes 45897 -19 57 17 3:1 0.7864 Yes 45897 -2 56 11 3:1 0.0921 Yes 45897 -20 65 19 3:1 0.6143 Yes 45897 -3 64 1 63:1 1 Yes 45897 -4 63 15 3:1 0.2914 Yes C. sativa C19 65416 -1 97 34 3:1 0.84 Accept 65416 -2 59 22 3:1 0.61 Accept 65416 -3 81 37 3:1 0.09 Accept 65416 -4 69 45 3:1 0.0002 No 65416 -5 174 47 3:1 0.213 Accept 65416 -6 176 37 3:1 0.01 No 65416 -7 99 19 3:1 0.03 No 65416 -8 123 26 3:1 0.04 No 65416 -9 110 18 15:1 0.0002 No 65416 -10 153 14 15:1 0.192 Accept 65416 -11 97 35 3:1 0.688 Accept 65416 -12 102 7 15:1 1 Accept 65416 -13 92 33 3:1 0.679 Accept 65416 -14 113 71 3:1 0 No 65416 -15 120 48 3:1 0.285 Accept 65416 -16 106 60 3:1 0.0006 No 65416 -17 203 63 3:1 0.67 Accept 65416 -19 165 52 3:1 0.75 Accept 65416 -20 40 11 3:1 0.52 Accept 65416 -21 261 78 3:1 0.38 Accept

TABLE-US-00008 Average fatty acid composition (%) in transgenic T3 plants. Complete data in Appendix 2. LINE 16:0 18:0 18:1 18:2 GLA 18:3 20:1 DGLA SDA ETA GLA DGLA SDA ETA ALA SDA ETA GLA DGLA 1A 8.96 ±0.52 3.69 ±0.15 31.64 ±1.49 6.58 ±0.99 3.40 ±0.25 6.33 ±0.54 17.08 ±0.87 7.49 ±0.85 0.55 ±0.23 1.40 ±0.22 12.83 8.27 10.89 1C 8.38 ±0.18 3.65 ±0.09 32.33 ±0.59 11.90 ±3.22 3.11 ±0.69 9.50 ±1.22 18.67 ±0.44 2.03 ±0.92 0.91 ±0.22 0.38 ±0.26 6.43 10.78 5.15 2B 8.44 ±0.61 3.54 ±0.15 30.31 ±2.20 10.02 ±2.27 4.07 ±0.81 8.44 ±2.00 17.74 ±1.04 3.32 ±2.22 0.79 ±0.15 0.43 ±0.37 8.61 9.66 7.39 2C 8.36 ±0.41 3.58 ±0.22 31.10 ±1.70 8.79 ±2.63 3.80 ±0.62 8.22 ±2.67 18.15 ±0.92 3.69 ±2.99 0.80 ±0.13 0.66 ±0.56 8.96 9.69 7.50 3A 8.25 ±0.85 3.59 ±0.54 13.86 ±3.03 19.52 ±1.92 8.00 ±1.21 12.18 ±1.50 17.82 ±1.32 1.56 ±0.63 1.09 ±0.20 0.00 10.64 13.27 9.55 3B 8.69 ±0.32 3.25 ±0.07 12.57 ±1.82 18.48 ±3.35 11.59 ±2.99 12.28 ±2.02 16.40 ±0.82 1.25 ±0.74 1.77 ±0.92 0.00 14.60 14.05 12.83 3C 8.13 ±0.27 3.59 ±0.07 14.32 ±1.09 15.58 ±4.40 11.53 ±3.97 10.11 ±2.36 17.74 ±0.82 3.33 ±3.08 1.63 ±0.67 0.28 ±0.48 16.77 12.02 14.86 5A 8.33 ±0.12 3.10 ±0.12 12.15 ±0.79 23.54 ±6.02 8.90 ±1.49 13.64 ±0.59 16.45 ±0.27 1.68 ±0.69 1.18 ±0.32 0.00 11.77 14.83 10.58 5B 8.86 ±0.36 3.28 ±0.17 14.61 ±8.96 15.65 ±4.73 12.70 ±1.84 10.67 ±2.29 16.00 ±1.13 1.71 ±1.53 1.85 ±0.31 0.01 ±0.01 16.26 12.53 14.41 5C 7.73 ±0.24 2.95 ±0.07 14.59 ±1.10 22.07 ±0.99 6.90 ±1.20 13.85 ±0.87 18.00 ±0.43 0.87 ±0.49 0.94 ±0.18 0.00 8.70 14.79 7.76 C1 7.82 ±0.13 3.27 ±0.15 12.89 ±2.12 11.53 ±7.13 15.07 ±7.82 9.42 ±3.98 17.21 ±0.42 5.79 ±3.13 2.40 ±1.26 0.76 ±0.51 24.02 12.58 20.85 C15 7.96 ±0.06 2.96 ±0.06 13.35 ±0.73 21.49 ±0.44 8.29 ±0.74 12.94 ±0.43 17.49 ±0.09 1.49 ±0.39 1.11 ±0.13 0.02 ±0.04 10.91 14.07 9.78 C19 7.70 ±0.24 3.71 ±0.17 14.60 ±1.62 7.50 ±0.89 16.99 ±2.36 7.81 ±0.74 16.96 ±0.44 6.12 ±0.67 2.57 ±0.46 0.87 ±0.14 26.55 11.25 23.11

TABLE-US-00009 PDCT Name SEQ ID NA SEQ ID AA Activity Organism Napus_1A 1 2 PDCT1 Brasssica napus Napus_2A 3 4 PDCT1 Brasssica napus Carinata_1B 5 6 PDCT1 Brassica carinata Carinata_1C 7 8 PDCT1 Brassica carinata Carinata_2B 9 10 PDCT1 Brassica carinata Carinata_2C 11 12 PDCT1 Brassica carinata BjROD1-B4 13 14 PDCT1 Brassica juncea BjROD1-A3 15 16 PDCT1 Brassica juncea BjROD1-B3 39 40 PDCT1 Brassica juncea Napus_1C 41 42 PDCT1 Brasssica napus Napus_2C 43 44 PDCT1 Brasssica napus Consensus PDCT1 45 46 PDCT1 Artificial Napus_3A 17 18 PDCT3/5 Brasssica napus Napus_5A 19 20 PDCT3/5 Brasssica napus Carinata_3B 21 22 PDCT3/5 Brassica carinata Carinata_3C 23 24 PDCT3/5 Brassica carinata Carinata_5B 25 26 PDCT3/5 Brassica carinata Carinata_5C 27 28 PDCT3/5 Brassica carinata BjROD1-A2 29 30 PDCT3/5 Brassica juncea BjROD1-B2 31 32 PDCT3/5 Brassica juncea BjROD1-B1 49 50 PDCT3/5 Brassica juncea BjROD1-A1 51 52 PDCT3/5 Brassica juncea BrROD1_SEQIDNO7 53 54 PDCT3/5 Brassica rapa Napus_5C 55 56 PDCT3/5 Brasssica napus Napus_3C 57 58 PDCT3/5 Brasssica napus Consensus PDCT3/5 59 60 PDCT3/5 Artificial Camelina_C15(45897) 33 34 PDCT15 Camelina sativa Camelina_C19(65416) 35 36 PDCT19 Camelina sativa Camelina_C1(80666) 37 38 PDCT19 Camelina sativa Consensus PDCT19 47 48 PCDT19 Artificial AtRodD1 61 62 Arabidopsis thaliana GmROD1-1 63 64 PDCT1 candiate Glycine max GmROD1-2 65 66 PDCT1 candiate Glycine max RcPDCT 67 68 PDCT1 candiate Ricinis communis RcROD1_SEQIDNO9 69 70 PDCT1 candiate Ricinis communis LuPDCT1 71 72 PDCT1 candiate Linum usitatissimum LuPDCT2 73 74 PDCT1 candiate Linum usitatissimum OsROD1_SEQIDNO 11 75 76 / Oryza sativa ZmROD1_GRMZM2G015040 77 78 / Zea mays ZmROD1_GRMZM2G087896 78 80 / Zea mays

TABLE-US-00010 Seq 1 Seq 2 Needle Protein Identity% Default settings ATRODD1 ATRODD1 100 ATRODD1 BJROD1-A1 78,8 ATRODD1 BJROD1-A2 76,1 ATRODD1 BJROD1-A3 72,7 ATRODD1 BJROD1-B1 78,5 ATRODD1 BJROD1-B2 78,1 ATRODD1 BJROD1-B3 73,7 ATRODD1 BJROD1-B4 55,5 ATRODD1 BRROD1_SEQID NO7 78,8 ATRODD1 CAMELINA_C1(8 0666) 86,1 ATRODD1 CAMELINA_C15( 45897) 85,8 ATRODD1 CAMELINA_C19( 65416) 86,2 ATRODD1 CARINATA 1B 73,7 ATRODD1 CARINATA 1C 74 ATRODD1 CARINATA 2B 55,5 ATRODD1 CARINATA 2C 55,5 ATRODD1 CARINATA 3B 78,5 ATRODD1 CARINATA 3C 78,8 ATRODD1 CARINATA 5B 80,5 ATRODD1 CARINATA 5C 79,8 ATRODD1 GMROD1-1 60,7 ATRODD1 GMROD1-2 58,1 ATRODD1 LUPDCT1 54,6 ATRODD1 LUPDCT2 54,2 ATRODD1 NAPUS 1A 72,7 ATRODD1 NAPUS 1C 73,7 ATRODD1 NAPUS 2A 55,5 ATRODD1 NAPUS 2C 55,1 ATRODD1 NAPUS 3A 79,2 ATRODD1 NAPUS 3C 78,8 ATRODD1 NAPUS 5A 79,7 ATRODD1 NAPUS 5C 80,1 BJROD1-A1 ZMROD1_GRMZ M2G087896 44,1 BJROD1-A2 ATRODD1 76,1 BJROD1-A2 BJROD1-A1 82,6 BJROD1-A2 BJROD1-A2 100 BJROD1-A2 BJROD1-A3 77,2 BJROD1-A2 BJROD1-B1 83,3 BJROD1-A2 BJROD1-B2 87 BJROD1-A2 BJROD1-B3 77,9 BJROD1-A2 BJROD1-B4 55,4 BJROD1-A2 BRROD1_SEQID NO7 83 BJROD1-A2 CAMELINA_C1(8 0666) 71,7 BJROD1-A2 CAMELINA_C15( 45897) 71,9 BJROD1-A2 CAMELINA_C19( 65416) 72,5 BJROD1-A2 CARINATA 1B 77,9 BJROD1-A2 CARINATA 1C 78,3 BJROD1-A2 CARINATA 2B 55,4 BJROD1-A2 CARINATA 2C 55,4 BJROD1-A2 CARINATA 3B 83,3 BJROD1-A2 CARINATA 3C 83 BJROD1-A2 CARINATA 5B 88,8 BJROD1-A2 CARINATA 5C 93,3 BJROD1-A2 GMROD1-1 62,1 BJROD1-A2 GMROD1-2 57,5 BJROD1-A2 LUPDCT1 51,5 BJROD1-A2 LUPDCT2 51,5 BJROD1-A2 NAPUS 1A 76,6 BJROD1-A2 NAPUS 1C 77,9 BJROD1-A2 NAPUS 2A 55,4 BJROD1-A2 NAPUS 2C 55,1 BJROD1-A2 NAPUS 3A 81,7 BJROD1-A2 NAPUS 3C 83 BJROD1-A2 NAPUS 5A 95,4 BJROD1-A2 NAPUS 5C 93,6 BJROD1-A2 OSROD1_SEQID NO 11 42,2 BJROD1-A2 RCPDCT 59,7 BJROD1-A2 RCROD1_SEQID NO9 59,7 BJROD1-A2 ZMROD1_GRMZ M2G015040 45,1 BJROD1-A2 ZMROD1_GRMZ M2G087896 45,6 BJROD1-A3 ATRODD1 72,7 BJROD1-A3 BJROD1-A1 77,8 BJROD1-A3 BJROD1-A2 77,2 BJROD1-A3 BJROD1-A3 100 BJROD1-B1 CAMELINA_C1(8 0666) 76,5 BJROD1-B1 CAMELINA_C15( 45897) 75,8 BJROD1-B1 CAMELINA_C19( 65416) 75,8 BJROD1-B1 CARINATA 1B 78,5 BJROD1-B1 CARINATA 1C 79,2 BJROD1-B1 CARINATA 2B 56,8 BJROD1-B1 CARINATA 2C 56,8 BJROD1-B1 CARINATA 3B 99,3 BJROD1-B1 CARINATA 3C 98,2 BJROD1-B1 CARINATA 5B 86,8 BJROD1-B1 CARINATA 5C 86,8 BJROD1-B1 GMROD1-1 61,5 BJROD1-B1 GMROD1-2 62,8 BJROD1-B1 LUPDCT1 53,9 BJROD1-B1 LUPDCT2 53,9 BJROD1-B1 NAPUS 1A 77,8 BJROD1-B1 NAPUS 1C 79,2 BJROD1-B1 NAPUS 2A 56,8 BJROD1-B1 NAPUS 2C 56,4 BJROD1-B1 NAPUS 3A 96,8 BJROD1-B1 NAPUS 3C 98,2 BJROD1-B1 NAPUS 5A 86,8 BJROD1-B1 NAPUS 5C 87,2 BJROD1-B1 OSROD1_SEQID NO 11 43,8 BJROD1-B1 RCPDCT 60,8 BJROD1-B1 RCROD1_SEQID NO9 60,8 BJROD1-B1 ZMROD1_GRMZ M2G015040 45,8 BJROD1-B1 ZMROD1_GRMZ M2G087896 44,1 BJROD1-B2 ATRODD1 78,1 BJROD1-B2 BJROD1-A1 83,7 BJROD1-B2 BJROD1-A2 87 BJROD1-B2 BJROD1-A3 76,1 BJROD1-B2 BJROD1-B1 83,3 BJROD1-B2 BJROD1-B2 100 BJROD1-B2 BJROD1-B3 77,5 BJROD1-B2 BJROD1-B4 56,1 BJROD1-B2 BRROD1_SEQID NO7 84 BJROD1-B2 CAMELINA_C1(8 0666) 75,1 BJROD1-B2 CAMELINA_C15( 45897) 75,2 BJROD1-B2 CAMELINA_C19( 65416) 75,2 BJROD1-B2 CARINATA 1B 77,1 BJROD1-B3 CARINATA 5B 80,2 BJROD1-B3 CARINATA 5C 78,8 BJROD1-B3 GMROD1-1 61,5 BJROD1-B3 GMROD1-2 52,7 BJROD1-B3 LUPDCT1 53,3 BJROD1-B3 LUPDCT2 53,6 BJROD1-B3 NAPUS 1A 95,2 BJROD1-B3 NAPUS 1C 95,5 BJROD1-B3 NAPUS 2A 56,1 BJROD1-B3 NAPUS 2C 55,7 BJROD1-B3 NAPUS 3A 78,3 BJROD1-B3 NAPUS 3C 78,8 BJROD1-B3 NAPUS 5A 78,9 BJROD1-B3 NAPUS 5C 79,2 BJROD1-B3 OSROD1_SEQID NO 11 43,2 BJROD1-B3 RCPDCT 57,6 BJROD1-B3 RCROD1_SEQID NO9 57,6 BJROD1-B3 ZMROD1_GRMZ M2G015040 44 BJROD1-B3 ZMROD1_GRMZ M2G087896 44,7 BJROD1-B4 ATRODD1 55,5 BJROD1-B4 BJROD1-A1 57,1 BJROD1-B4 BJROD1-A2 55,4 BJROD1-B4 BJROD1-A3 55,4 BJROD1-B4 BJROD1-B1 56,8 BJROD1-B4 BJROD1-B2 59 BJROD1-B4 BJROD1-B3 56,1 BJROD1-B4 BJROD1-B4 100 BJROD1-B4 BRROD1_SEQID NO7 57,1 BJROD1-B4 CAMELINA_C1(8 0666) 55,4 BJROD1-B4 CAMELINA_C15( 45897) 55,2 BJROD1-B4 CAMELINA_C19( 65416) 55,2 BJROD1-B4 CARINATA 1B 56,4 BJROD1-B4 CARINATA 1C 55,4 BJROD1-B4 CARINATA 2B 97,4 BJROD1-B4 CARINATA 2C 98,3 BJROD1-B4 CARINATA 3B 57,1 BJROD1-B4 CARINATA 3C 56,8 BJROD1-B4 CARINATA 5B 58,2 BJROD1-B4 CARINATA 5C 55,5 BJROD1-B4 GMROD1-1 54,9 BJROD1-B4 GMROD1-2 53,5 BJROD1-B4 LUPDCT1 48,4 BRROD1_SEQID NO7 GMROD1-2 63,1 BRROD1_SEQID NO7 LUPDCT1 54,5 BRROD1_SEQID NO7 LUPDCT2 54,5 BRROD1_SEQID NO7 NAPUS 1A 77,8 BRROD1_SEQID NO7 NAPUS_1C 79,2 BRROD1_SEQID NO7 NAPUS 2A 57,1 BRROD1_SEQID NO7 NAPUS 2C 56,8 BRROD1_SEQID NO7 NAPUS 3A 98,9 BRROD1_SEQID NO7 NAPUS 3C 98,6 BRROD1_SEQID NO7 NAPUS 5A 86,8 BRROD1_SEQID NO7 NAPUS 5C 87,2 BRROD1_SEQID NO7 OSROD1_SEQID NO 11 41,4 BRROD1_SEQID NO7 RCPDCT 60,8 BRROD1_SEQID NO7 RCROD1_SEQID NO9 60,8 BRROD1_SEQID NO7 ZMROD1_GRMZ M2G015040 46,2 BRROD1_SEQID NO7 ZMROD1_GRMZ M2G087896 44,1 CAMELINA_C1(8 0666) ATRODD1 86,1 CAMELINA_C1(8 0666) BJROD1-A1 76,8 CAMELINA_C1(8 0666) BJROD1-A2 71,7 CAMELINA_C1(8 0666) BJROD1-A3 69,1 CAMELINA_C1(8 0666) BJROD1-B1 76,5 CAMELINA_C1(8 0666) BJROD1-B2 75,1 CAMELINA_C1(8 0666) BJROD1-B3 70,4 CAMELINA_C1(8 0666) BJROD1-B4 55,4 CAMELINA_C1(8 0666) BRROD1_SEQID NO7 76,8 CAMELINA_C1(8 0666) CAMELINA_C1 (8 0666) 100 CAMELINA_C1(8 0666) CAMELINA_C15( 45897) 96,6 CAMELINA_C1(8 0666) CAMELINA_C19( 65416) 98 CAMELINA_C1(8 0666) CARINATA 1B 70,4 CAMELINA_C1(8 0666) CARINATA 1C 71,1 CAMELINA_C1(8 0666) CARINATA 2B 55,4 CAMELINA_C1(8 0666) CARINATA 2C 55,4 CAMELINA_C15( 45897) CAMELINA_C19( 65416) 97,3 CAMELINA_C15( 45897) CARINATA 1B 70,2 CAMELINA_C15( 45897) CARINATA 1C 70,9 CAMELINA_C15( 45897) CARINATA 2B 55,2 CAMELINA_C15( 45897) CARINATA 2C 55,2 CAMELINA_C15( 45897) CARINATA 3B 75,8 CAMELINA_C15( 45897) CARINATA 3C 76,2 CAMELINA_C15( 45897) CARINATA 5B 76,8 CAMELINA_C15( 45897) CARINATA 5C 76,6 CAMELINA_C15( 45897) GMROD1-1 61 CAMELINA_C15( 45897) GMROD1-2 60,7 CAMELINA_C15( 45897) LUPDCT1 53,9 CAMELINA_C15( 45897) LUPDCT2 54,2 CAMELINA_C15( 45897) NAPUS 1A 69,5 CAMELINA_C15( 45897) NAPUS_1C 70,5 CAMELINA_C15( 45897) NAPUS 2A 55,2 CAMELINA_C15( 45897) NAPUS 2C 54,9 CAMELINA_C15( 45897) NAPUS 3A 76,5 CAMELINA_C15( 45897) NAPUS 3C 76,2 CAMELINA_C15( 45897) NAPUS 5A 75,6 CAMELINA_C15( 45897) NAPUS 5C 76,9 CAMELINA_C15( 45897) OSROD1_SEQID NO 11 45,4 CAMELINA_C15( 45897) RCPDCT 59,8 CAMELINA_C15( 45897) RCROD1_SEQID NO9 59,8 CAMELINA_C15( 45897) ZMROD1_GRMZ M2G015040 45 CAMELINA_C15( 45897) ZMROD1_GRMZ M2G087896 46,5 CAMELINA_C19( 65416) ATRODD1 86,2 CAMELINA_C19( 65416) BJROD1-A1 76,5 CAMELINA_C19( 65416) BJROD1-A2 72,5 CAMELINA_C19( 65416) BJROD1-A3 69,5 CAMELINA_C19( 65416) BJROD1-B1 75,8 CAMELINA_C19( 65416) BJROD1-B2 75,2 CARINATA 1B BJROD1-A2 77,9 CARINATA 1B BJROD1-A3 95,8 CARINATA 1B BJROD1-B1 78,5 CARINATA 1B BJROD1-B2 77,1 CARINATA 1B BJROD1-B3 98,6 CARINATA 1B BJROD1-B4 56,4 CARINATA 1B BRROD1_SEQID NO7 78,5 CARINATA 1B CAMELINA_C1(8 0666) 70,4 CARINATA 1B CAMELINA_C15( 45897) 70,2 CARINATA 1B CAMELINA_C19( 65416) 70,9 CARINATA 1B CARINATA 1B 100 CARINATA 1B CARINATA 1C 93,8 CARINATA 1B CARINATA 2B 56,4 CARINATA 1B CARINATA 2C 56,7 CARINATA 1B CARINATA 3B 78,8 CARINATA 1B CARINATA 3C 78,8 CARINATA 1B CARINATA 5B 80,2 CARINATA 1B CARINATA 5C 78,8 CARINATA 1B GMROD1-1 61,1 CARINATA 1B GMROD1-2 55,3 CARINATA 1B LUPDCT1 54,1 CARINATA 1B LUPDCT2 54,5 CARINATA 1B NAPUS 1A 94,5 CARINATA 1B NAPUS_1C 94,8 CARINATA 1B NAPUS 2A 56,4 CARINATA 1B NAPUS 2C 56,1 CARINATA 1B NAPUS 3A 78,3 CARINATA 1B NAPUS 3C 78,8 CARINATA 1B NAPUS 5A 78,9 CARINATA 1B NAPUS 5C 79,2 CARINATA 1B OSROD1_SEQID NO 11 42,3 CARINATA 1B RCPDCT 57,6 CARINATA 1B RCROD1_SEQID NO9 57,6 CARINATA 1B ZMROD1_GRMZ M2G015040 44 CARINATA 1B ZMROD1_GRMZ M2G087896 44,7 CARINATA 1C ATRODD1 74 CARINATA 1C BJROD1-A1 78,8 CARINATA 1C BJROD1-A2 78,3 CARINATA 1C BJROD1-A3 94,5 CARINATA 1C BJROD1-B1 79,2 CARINATA 1C BJROD1-B2 77,5 CARINATA 1C BJROD1-B3 94,5 CARINATA 2B CAMELINA_C19( 65416) 55,2 CARINATA 2B CARINATA 1B 56,4 CARINATA 2B CARINATA 1C 55,4 CARINATA 2B CARINATA 2B 100 CARINATA 2B CARINATA 2C 99,1 CARINATA 2B CARINATA 3B 57,1 CARINATA 2B CARINATA 3C 56,8 CARINATA 2B CARINATA 5B 58,2 CARINATA 2B CARINATA 5C 55,5 CARINATA 2B GMROD1-1 55,7 CARINATA 2B GMROD1-2 54,6 CARINATA 2B LUPDCT1 49,3 CARINATA 2B LUPDCT2 49,3 CARINATA 2B NAPUS 1A 55 CARINATA 2B NAPUS 1C 55,7 CARINATA 2B NAPUS 2A 97 CARINATA 2B NAPUS 2C 96,6 CARINATA 2B NAPUS 3A 57,4 CARINATA 2B NAPUS 3C 56,8 CARINATA 2B NAPUS 5A 55,5 CARINATA 2B NAPUS 5C 55,5 CARINATA 2B OSROD1_SEQID NO 11 38,1 CARINATA 2B RCPDCT 52,3 CARINATA 2B RCROD1_SEQID NO9 52,3 CARINATA 2B ZMROD1_GRMZ M2G015040 44,9 CARINATA 2B ZMROD1_GRMZ M2G087896 44,2 CARINATA 2C ATRODD1 55,5 CARINATA 2C BJROD1-A1 57,1 CARINATA 2C BJROD1-A2 55,4 CARINATA 2C BJROD1-A3 55,4 CARINATA 2C BJROD1-B1 56,8 CARINATA 2C BJROD1-B2 59 CARINATA 2C BJROD1-B3 56,4 CARINATA 2C BJROD1-B4 98,3 CARINATA 2C BRROD1_SEQID NO7 57,1 CARINATA 2C CAMELINA_C1(8 0666) 55,4 CARINATA 2C CAMELINA_C15( 45897) 55,2 CARINATA 2C CAMELINA_C19( 65416) 55,2 CARINATA 2C CARINATA 1B 56,7 CARINATA 2C CARINATA 1C 55,7 CARINATA 2C CARINATA 2B 99,1 CARINATA 2C CARINATA 2C 100 CARINATA 3B GMROD1-2 63,1 CARINATA 3B LUPDCT1 54,2 CARINATA 3B LUPDCT2 54,2 CARINATA 3B NAPUS 1A 78,2 CARINATA 3B NAPUS 1C 79,5 CARINATA 3B NAPUS 2A 57,1 CARINATA 3B NAPUS 2C 56,8 CARINATA 3B NAPUS 3A 96,8 CARINATA 3B NAPUS 3C 98,2 CARINATA 3B NAPUS 5A 86,8 CARINATA 3B NAPUS 5C 87,2 CARINATA 3B OSROD1_SEQID NO 11 44,1 CARINATA 3B RCPDCT 61,1 CARINATA 3B RCROD1_SEQID NO9 61,1 CARINATA 3B ZMROD1_GRMZ M2G015040 46,4 CARINATA 3B ZMROD1_GRMZ M2G087896 44,4 CARINATA 3C ATRODD1 78,8 CARINATA 3C BJROD1-A1 97,9 CARINATA 3C BJROD1-A2 83 CARINATA 3C BJROD1-A3 78,8 CARINATA 3C BJROD1-B1 98,2 CARINATA 3C BJROD1-B2 85,2 CARINATA 3C BJROD1-B3 78,8 CARINATA 3C BJROD1-B4 56,8 CARINATA 3C BRROD1_SEQID NO7 98,6 CARINATA 3C CAMELINA_C1(8 0666) 76,8 CARINATA 3C CAMELINA_C15( 45897) 76,2 CARINATA 3C CAMELINA _C19( 65416) 76,5 CARINATA 3C CARINATA 1B 78,8 CARINATA 3C CARINATA 1C 79,9 CARINATA 3C CARINATA 2B 56,8 CARINATA 3C CARINATA 2C 56,8 CARINATA 3C CARINATA 3B 98,2 CARINATA 3C CARINATA 3C 100 CARINATA 3C CARINATA 5B 87,1 CARINATA 3C CARINATA 5C 86,8 CARINATA 3C GMROD1-1 61,9 CARINATA 3C GMROD1-2 63,1 CARINATA 3C LUPDCT1 54,5 CARINATA 3C LUPDCT2 54,5 CARINATA 3C NAPUS 1A 78,2 CARINATA 3C NAPUS 1C 79,5 CARINATA 5B NAPUS 5C 94,1 CARINATA 5B OSROD1_SEQID NO 11 42,6 CARINATA 5B RCPDCT 59,9 CARINATA 5B RCROD1_SEQID NO9 59,9 CARINATA 5B ZMROD1_GRMZ M2G015040 46,4 CARINATA 5B ZMROD1_GRMZ M2G087896 45,8 CARINATA 5C ATRODD1 79,8 CARINATA 5C BJROD1-A1 86,5 CARINATA 5C BJROD1-A2 93,3 CARINATA 5C BJROD1-A3 78,2 CARINATA 5C BJROD1-B1 86,8 CARINATA 5C BJROD1-B2 91,2 CARINATA 5C BJROD1-B3 78,8 CARINATA 5C BJROD1-B4 55,5 CARINATA 5C BRROD1_SEQID NO7 86,8 CARINATA 5C CAMELINA_C1(8 0666) 75,7 CARINATA 5C CAMELINA_C15( 45897) 76,6 CARINATA 5C CAMELINA_C19( 65416) 76,1 CARINATA 5C CARINATA 1B 78,8 CARINATA 5C CARINATA 1C 79,2 CARINATA 5C CARINATA 2B 55,5 CARINATA 5C CARINATA 2C 55,5 CARINATA 5C CARINATA 3B 86,8 CARINATA 5C CARINATA 3C 86,8 CARINATA 5C CARINATA 5B 93,7 CARINATA 5C CARINATA 5C 100 CARINATA 5C GMROD1-1 60,3 CARINATA 5C GMROD1-2 61,1 CARINATA 5C LUPDCT1 51,7 CARINATA 5C LUPDCT2 51,7 CARINATA 5C NAPUS 1A 77,5 CARINATA 5C NAPUS 1C 78,8 CARINATA 5C NAPUS 2A 55,5 CARINATA 5C NAPUS 2C 55,1 CARINATA 5C NAPUS 3A 85,5 CARINATA 5C NAPUS 3C 86,8 CARINATA 5C NAPUS 5A 97,5 CARINATA 5C NAPUS 5C 99,6 CARINATA 5C OSROD1_SEQID NO 11 42,5 CARINATA 5C RCPDCT 59,9 CARINATA 5C RCROD1_SEQID NO9 59,9 GMROD1-2 BJROD1-A2 57,5 GMROD1-2 BJROD1-A3 54,4 GMROD1-2 BJROD1-B1 62,8 GMROD1-2 BJROD1-B2 65 GMROD1-2 BJROD1-B3 52,7 GMROD1-2 BJROD1-B4 53,5 GMROD1-2 BRROD1_SEQID NO7 63,1 GMROD1-2 CAMELINA_C1(8 0666) 60,1 GMROD1-2 CAMELINA_C15( 45897) 60,7 GMROD1-2 CAMELINA_C19( 65416) 60,5 GMROD1-2 CARINATA 1B 55,3 GMROD1-2 CARINATA 1C 52,9 GMROD1-2 CARINATA 2B 54,6 GMROD1-2 CARINATA 2C 53,9 GMROD1-2 CARINATA 3B 63,1 GMROD1-2 CARINATA 3C 63,1 GMROD1-2 CARINATA 5B 61,2 GMROD1-2 CARINATA 5C 61,1 GMROD1-2 GMROD1-1 86,3 GMROD1-2 GMROD1-2 100 GMROD1-2 LUPDCT1 56,1 GMROD1-2 LUPDCT2 56,1 GMROD1-2 NAPUS 1A 54,4 GMROD1-2 NAPUS 1C 52,9 GMROD1-2 NAPUS 2A 53,5 GMROD1-2 NAPUS 2C 53,2 GMROD1-2 NAPUS 3A 62,7 GMROD1-2 NAPUS 3C 63,1 GMROD1-2 NAPUS 5A 61 GMROD1-2 NAPUS 5C 61,1 GMROD1-2 OSROD1_SEQID NO 11 46,5 GMROD1-2 RCPDCT 59,3 GMROD1-2 RCROD1_SEQID NO9 59,3 GMROD1-2 ZMROD1_GRMZ M2G015040 50,9 GMROD1-2 ZMROD1_GRMZ M2G087896 49 LUPDCT1 ATRODD1 54,6 LUPDCT1 BJROD1-A1 54,5 LUPDCT1 BJROD1-A2 51,5 LUPDCT1 BJROD1-A3 52,5 LUPDCT1 BJROD1-B1 53,9 LUPDCT1 BJROD1-B2 53,8 LUPDCT1 BJROD1-B3 53,3 LUPDCT2 CAMELINA_C19( 65416) 55,3 LUPDCT2 CARINATA 1B 54,5 LUPDCT2 CARINATA 1C 53,6 LUPDCT2 CARINATA 2B 49,3 LUPDCT2 CARINATA 2C 48,7 LUPDCT2 CARINATA 3B 54,2 LUPDCT2 CARINATA 3C 54,5 LUPDCT2 CARINATA 5B 54,1 LUPDCT2 CARINATA 5C 51,7 LUPDCT2 GMROD1-1 60,1 LUPDCT2 GMROD1-2 56,1 LUPDCT2 LUPDCT1 98,6 LUPDCT2 LUPDCT2 100 LUPDCT2 NAPUS 1A 53,8 LUPDCT2 NAPUS 1C 53,6 LUPDCT2 NAPUS 2A 48,4 LUPDCT2 NAPUS 2C 48 LUPDCT2 NAPUS 3A 54,9 LUPDCT2 NAPUS 3C 54,5 LUPDCT2 NAPUS 5A 52 LUPDCT2 NAPUS 5C 52 LUPDCT2 OSROD1_SEQID NO 11 46,3 LUPDCT2 RCPDCT 59,2 LUPDCT2 RCROD1_SEQID NO9 59,2 LUPDCT2 ZMROD1_GRMZ M2G015040 47,8 LUPDCT2 ZMROD1_GRMZ M2G087896 48,6 NAPUS 1A ATRODD1 72,7 NAPUS 1A BJROD1-A1 77,1 NAPUS 1A BJROD1-A2 76,6 NAPUS 1A BJROD1-A3 98,6 NAPUS 1A BJROD1-B1 77,8 NAPUS 1A BJROD1-B2 75,4 NAPUS 1A BJROD1-B3 95,2 NAPUS 1A BJROD1-B4 55,4 NAPUS 1A BRROD1_SEQID NO7 77,8 NAPUS 1A CAMELINA_C1(8 0666) 69,1 NAPUS 1A CAMELINA_C15( 45897) 69,5 NAPUS 1A CAMELINA_C19( 65416) 69,5 NAPUS 1A CARINATA 1B 94,5 NAPUS 1A CARINATA 1C 95,5 NAPUS 1A CARINATA 2B 55 NAPUS 1A CARINATA 2C 55,4 NAPUS 1C GMROD1-2 52,9 NAPUS 1C LUPDCT1 53,3 NAPUS 1C LUPDCT2 53,6 NAPUS 1C NAPUS 1A 96,5 NAPUS 1C NAPUS 1C 100 NAPUS 1C NAPUS 2A 55,7 NAPUS 1C NAPUS 2C 55,4 NAPUS 1C NAPUS 3A 79 NAPUS 1C NAPUS 3C 79,5 NAPUS 1C NAPUS 5A 78,9 NAPUS 1C NAPUS 5C 79,2 NAPUS 1C OSROD1_SEQID NO 11 42 NAPUS 1C RCPDCT 57,9 NAPUS 1C RCROD1_SEQID NO9 57,9 NAPUS 1C ZMROD1_GRMZ M2G015040 43,3 NAPUS 1C ZMROD1_GRMZ M2G087896 43 NAPUS 2A ATRODD1 55,5 NAPUS 2A BJROD1-A1 57,1 NAPUS 2A BJROD1-A2 55,4 NAPUS 2A BJROD1-A3 55,4 NAPUS 2A BJROD1-B1 56,8 NAPUS 2A BJROD1-B2 59 NAPUS 2A BJROD1-B3 56,1 NAPUS 2A BJROD1-B4 99,6 NAPUS 2A BRROD1_SEQID NO7 57,1 NAPUS 2A CAMELINA_C1(8 0666) 55,4 NAPUS 2A CAMELINA_C15( 45897) 55,2 NAPUS 2A CAMELINA_C19( 65416) 55,2 NAPUS 2A CARINATA 1B 56,4 NAPUS 2A CARINATA 1C 55,4 NAPUS 2A CARINATA 2B 97 NAPUS 2A CARINATA 2C 97,9 NAPUS 2A CARINATA 3B 57,1 NAPUS 2A CARINATA 3C 56,8 NAPUS 2A CARINATA 5B 58,2 NAPUS 2A CARINATA 5C 55,5 NAPUS 2A GMROD1-1 54,9 NAPUS 2A GMROD1-2 53,5 NAPUS 2A LUPDCT1 48,4 NAPUS 2A LUPDCT2 48,4 NAPUS 2A NAPUS 1A 55,4 NAPUS 2A NAPUS 1C 55,7 NAPUS 2C NAPUS 5C 55,1 NAPUS 2C OSROD1_SEQID NO 11 38,1 NAPUS 2C RCPDCT 51,2 NAPUS 2C RCROD1_SEQID NO9 51,2 NAPUS 2C ZMROD1_GRMZ M2G015040 44,6 NAPUS 2C ZMROD1_GRMZ M2G087896 45,1 NAPUS 3A ATRODD1 79,2 NAPUS 3A BJROD1-A1 98,2 NAPUS 3A BJROD1-A2 81,7 NAPUS 3A BJROD1-A3 78,3 NAPUS 3A BJROD1-B1 96,8 NAPUS 3A BJROD1-B2 84,2 NAPUS 3A BJROD1-B3 78,3 NAPUS 3A BJROD1-B4 57,4 NAPUS 3A BRROD1_SEQID NO7 98,9 NAPUS 3A CAMELINA_C1 (8 0666) 76,9 NAPUS 3A CAMELINA_C15( 45897) 76,5 NAPUS 3A CAMELINA_C19( 65416) 77,2 NAPUS 3A CARINATA 1B 78,3 NAPUS 3A CARINATA 1C 79,3 NAPUS 3A CARINATA 2B 57,4 NAPUS 3A CARINATA 2C 57,4 NAPUS 3A CARINATA 3B 96,8 NAPUS 3A CARINATA 3C 98,2 NAPUS 3A CARINATA 5B 86,5 NAPUS 3A CARINATA 5C 85,5 NAPUS 3A GMROD1-1 61,2 NAPUS 3A GMROD1-2 62,7 NAPUS 3A LUPDCT1 54,9 NAPUS 3A LUPDCT2 54,9 NAPUS 3A NAPUS 1A 77,6 NAPUS 3A NAPUS 1C 79 NAPUS 3A NAPUS 2A 57,4 NAPUS 3A NAPUS 2C 57,1 NAPUS 3A NAPUS 3A 100 NAPUS 3A NAPUS 3C 98,2 NAPUS 3A NAPUS 5A 85,5 NAPUS 3A NAPUS 5C 85,9 NAPUS 3A OSROD1_SEQID NO 11 44,6 NAPUS 3A RCPDCT 61 NAPUS 3A RCROD1_SEQID NO9 61 NAPUS 5A BJROD1-A2 95,4 NAPUS 5A BJROD1-A3 78,2 NAPUS 5A BJROD1-B1 86,8 NAPUS 5A BJROD1-B2 90,8 NAPUS 5A BJROD1-B3 78,9 NAPUS 5A BJROD1-B4 55,5 NAPUS 5A BRROD1_SEQID NO7 86,8 NAPUS 5A CAMELINA_C1(8 0666) 75,3 NAPUS 5A CAMELINA_C15( 45897) 75,6 NAPUS 5A CAMELINA_C19( 65416) 76,2 NAPUS 5A CARINATA 1B 78,9 NAPUS 5A CARINATA 1C 79,3 NAPUS 5A CARINATA 2B 55,5 NAPUS 5A CARINATA 2C 55,5 NAPUS 5A CARINATA 3B 86,8 NAPUS 5A CARINATA 3C 86,8 NAPUS 5A CARINATA 5B 92,7 NAPUS 5A CARINATA 5C 97,5 NAPUS 5A GMROD1-1 62,3 NAPUS 5A GMROD1-2 61 NAPUS 5A LUPDCT1 52 NAPUS 5A LUPDCT2 52 NAPUS 5A NAPUS 1A 77,6 NAPUS 5A NAPUS 1C 78,9 NAPUS 5A NAPUS 2A 55,5 NAPUS 5A NAPUS 2C 55,1 NAPUS 5A NAPUS 3A 85,5 NAPUS 5A NAPUS 3C 86,8 NAPUS 5A NAPUS 5A 100 NAPUS 5A NAPUS 5C 97,9 NAPUS 5A OSROD1_SEQID NO 11 42,2 NAPUS 5A RCPDCT 60,2 NAPUS 5A RCROD1_SEQID NO9 60,2 NAPUS 5A ZMROD1_GRMZ M2G015040 45,2 NAPUS 5A ZMROD1_GRMZ M2G087896 45,6 NAPUS 5C ATRODD1 80,1 NAPUS 5C BJROD1-A1 86,8 NAPUS 5C BJROD1-A2 93,6 NAPUS 5C BJROD1-A3 78,5 NAPUS 5C BJROD1-B1 87,2 NAPUS 5C BJROD1-B2 91,5 NAPUS 5C BJROD1-B3 79,2 OSROD1_SEQID NO 11 BRROD1_SEQID NO7 41,4 OSROD1_SEQID NO 11 CAMELINA_C1(8 0666) 43,8 OSROD1_SEQID NO 11 CAMELINA_C15( 45897) 45,4 OSROD1_SEQID NO 11 CAMELINA_C19( 65416) 43,9 OSROD1_SEQID NO 11 CARINATA 1B 42,3 OSROD1_SEQID NO 11 CARINATA 1C 41,7 OSROD1_SEQID NO 11 CARINATA 2B 38,1 OSROD1_SEQID NO 11 CARINATA 2C 38,1 OSROD1_SEQID NO 11 CARINATA 3B 44,1 OSROD1_SEQID NO 11 CARINATA 3C 44,9 OSROD1_SEQID NO 11 CARINATA 5B 42,6 OSROD1_SEQID NO 11 CARINATA 5C 42,5 OSROD1_SEQID NO 11 GMROD1-1 47,1 OSROD1_SEQID NO 11 GMROD1-2 46,5 OSROD1_SEQID NO 11 LUPDCT1 45,9 OSROD1_SEQID NO 11 LUPDCT2 46,3 OSROD1_SEQID NO 11 NAPUS 1A 42,4 OSROD1_SEQID NO 11 NAPUS 1C 42 OSROD1_SEQID NO 11 NAPUS 2A 38,1 OSROD1_SEQID NO 11 NAPUS 2C 38,1 OSROD1_SEQID NO 11 NAPUS 3A 44,6 OSROD1_SEQID NO 11 NAPUS 3C 44,9 OSROD1_SEQID NO 11 NAPUS 5A 42,2 OSROD1_SEQID NO 11 NAPUS 5C 42,5 OSROD1_SEQID NO 11 OSROD1_SEQID NO 11 100 OSROD1_SEQID NO 11 RCPDCT 48,9 OSROD1_SEQID NO 11 RCROD1_SEQID NO9 48,9 OSROD1_SEQID NO 11 ZMROD1_GRMZ M2G015040 69,1 OSROD1_SEQID NO 11 ZMROD1_GRMZ M2G087896 68,9 RCPDCT ATRODD1 58,7 RCPDCT BJROD1-A1 58,6 RCPDCT BJROD1-A2 59,7 RCPDCT BJROD1-A3 57 RCROD1_SEQID NO9 BJROD1-B3 57,6 RCROD1_SEQID NO9 BJROD1-B4 51,6 RCROD1_SEQID NO9 BRROD1_SEQID NO7 60,8 RCROD1_SEQID NO9 CAMELINA_C1(8 0666) 55,4 RCROD1_SEQID NO9 CAMELINA_C15( 45897) 59,8 RCROD1_SEQID NO9 CAMELINA_C19( 65416) 59,9 RCROD1_SEQID NO9 CARINATA 1B 57,6 RCROD1_SEQID NO9 CARINATA 1C 57,9 RCROD1_SEQID NO9 CARINATA 2B 52,3 RCROD1_SEQID NO9 CARINATA 2C 51,9 RCROD1_SEQID NO9 CARINATA 3B 61,1 RCROD1_SEQID NO9 CARINATA 3C 60,8 RCROD1_SEQID NO9 CARINATA 5B 59,9 RCROD1_SEQID NO9 CARINATA 5C 59,9 RCROD1_SEQID NO9 GMROD1-1 68,2 RCROD1_SEQID NO9 GMROD1-2 59,3 RCROD1_SEQID NO9 LUPDCT1 59,2 RCROD1_SEQID NO9 LUPDCT2 59,2 RCROD1_SEQID NO9 NAPUS 1A 57,3 RCROD1_SEQID NO9 NAPUS 1C 57,9 RCROD1_SEQID NO9 NAPUS 2A 51,6 RCROD1_SEQID NO9 NAPUS 2C 51,2 RCROD1_SEQID NO9 NAPUS 3A 61 RCROD1_SEQID NO9 NAPUS 3C 60,8 RCROD1_SEQID NO9 NAPUS 5A 60,2 RCROD1_SEQID NO9 NAPUS 5C 59,9 RCROD1_SEQID NO9 OSROD1_SEQID NO 11 48,9 RCROD1_SEQID NO9 RCPDCT 100 RCROD1_SEQID NO9 RCROD1_SEQID NO9 100 RCROD1_SEQID NO9 ZMROD1_GRMZ M2G015040 51,3 RCROD1_SEQID NO9 ZMROD1_GRMZ M2G087896 48,2 ZMROD1_GRMZ M2G015040 ATRODD1 44,4 ZMROD1_GRMZ M2G015040 RCPDCT 51,3 ZMROD1_GRMZ M2G015040 RCROD1_SEQID NO9 51,3 ZMROD1_GRMZ M2G015040 ZMROD1_GRMZ M2G015040 100 ZMROD1_GRMZ M2G015040 ZMROD1_GRMZ M2G087896 83,9 ZMROD1_GRMZ M2G087896 ATRODD1 42,9 ZMROD1_GRMZ M2G087896 BJROD1-A1 44,1 ZMROD1_GRMZ M2G087896 BJROD1-A2 45,6 ZMROD1_GRMZ M2G087896 BJROD1-A3 42,7 ZMROD1_GRMZ M2G087896 BJROD1-B1 44,1 ZMROD1_GRMZ M2G087896 BJROD1-B2 45,6 ZMROD1_GRMZ M2G087896 BJROD1-B3 44,7 ZMROD1_GRMZ M2G087896 BJROD1-B4 43,6 ZMROD1_GRMZ M2G087896 BRROD1_SEQID NO7 44,1 ZMROD1_GRMZ M2G087896 CAMELINA_C1(8 0666) 47 ZMROD1_GRMZ M2G087896 CAMELINA_C15( 45897) 46,5 ZMROD1_GRMZ M2G087896 CAMELINA_C19( 65416) 47,7 ZMROD1_GRMZ M2G087896 CARINATA 1B 44,7 ZMROD1_GRMZ M2G087896 CARINATA 1C 42,7 ZMROD1_GRMZ M2G087896 CARINATA 2B 44,2 ZMROD1_GRMZ M2G087896 CARINATA 2C 44,7 ZMROD1_GRMZ M2G087896 CARINATA 3B 44,4 ZMROD1_GRMZ M2G087896 CARINATA 3C 44,4 ZMROD1_GRMZ M2G087896 CARINATA 5B 45,8 ZMROD1_GRMZ M2G087896 CARINATA 5C 44,8 ZMROD1_GRMZ M2G087896 GMROD1-1 53,1 ZMROD1_GRMZ M2G087896 GMROD1-2 49 ZMROD1_GRMZ M2G087896 LUPDCT1 49 ZMROD1_GRMZ M2G087896 LUPDCT2 48,6 ZMROD1_GRMZ M2G087896 NAPUS 1A 43 ZMROD1_GRMZ M2G087896 NAPUS 1C 43 ZMROD1_GRMZ M2G087896 NAPUS 2A 44 ZMROD1_GRMZ M2G087896 NAPUS 2C 43,6

TABLE-US-00011 Average fatty acid composition (%) in different lipid classes from immature seeds 16:0 18:0 18:1 18:2 GLA 18:3 SDA 20:0 20:1 20:2 DGLA 22:1 TAG 3C 10.3 5.0 18.7 15.2 17.3 7.2 2.3 2.4 15.2 1.1 3.6 1.3 5C 10.7 5.5 26.2 19.2 12.5 7.5 1.1 2.1 11.9 0.8 1.0 1.1 CK mutant 10.6 4.9 36.8 17.6 1.7 6.7 0.1 2.0 15.0 0.5 1.8 1.5 CK WT 9.4 4.7 19.5 22.0 11.6 7.9 1.6 2.2 16.6 1.2 1.2 1.4 WT 8.8 4.4 22.2 31.2 0.0 11.3 0.0 2.2 16.6 1.5 0.0 1.6 Rod mut 10.2 4.5 31.4 20.9 0.0 10.2 0.0 2.6 16.7 0.7 0.0 2.2 PC 16:0 18:0 18:1 18:2 GLA 18:3 SDA 20:0 20:1 20:2 DGLA 22:1 3C 19.0 2.8 6.1 37.5 6.8 23.0 1.1 0.1 1.2 1.0 1.0 0.2 5C 21.3 2.5 2.9 46.1 3.3 21.8 0.4 0.0 0.3 0.9 0.0 0.1 CK mutant 18.1 1.8 3.4 46.7 2.7 25.3 0.3 0.0 0.2 0.9 0.1 0.2 CK WT 27.3 3.6 4.0 40.0 3.9 18.3 0.5 0.0 0.7 0.9 0.2 0.0 WT 22.6 2.6 5.8 45.9 0.0 20.6 0.0 0.0 1.1 0.9 0.0 0.0 Rod mut 18.2 2.0 2.8 48.2 0.0 27.5 0.0 0.0 0.0 0.9 0.0 0.0 DAG 16:0 18:0 18:1 18:2 GLA 18:3 SDA 20:0 20:1 20:2 DGLA 22:0 3C 21.8 16.6 12.2 10.5 8.7 7.5 0.0 4.7 9.9 0.0 2.7 5.3 5C 24.6 14.4 11.4 13.7 7.1 6.8 0.0 6.6 6.0 0.0 0.0 9.2 CK mutant 19.9 10.3 32.9 16.2 1.1 5.0 0.0 3.2 10.4 0.0 0.0 0.8 CK WT 17.9 5.2 13.6 35.6 7.8 10.9 0.0 1.7 5.1 0.0 0.8 1.4 WT 17.1 7.2 24.4 34.1 0.0 5.9 0.0 2.4 6.7 0.0 0.0 2.2 Rod mut 18.2 9.7 25.1 19.2 0.0 7.3 0.0 5.2 9.9 0.0 0.0 5.4