BIO-NITROGEN FERTILIZER FORMULATION FOR ORGANIC AND SUSTAINABLE FARMING

Abstract

The present invention relates to the Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming. It particularly relates to the Bio-Nitrogen fertilizer formulation which reduces the use of chemical fertilizer and increase nutrients use efficiency and nitrogen bio-availability for better crop productivity and sustainable agriculture along with increasing the crop nutrition, crop productivity and soil fertility. It specifically relates to the Bio-Nitrogen fertilizer formulation containing nitrogen fixers from multiple sources such as Azotobacter sp., Azospirillum sp., Rhizobium sp., and Gluconacetobater diazotrophicus. The invention also relates to the process for preparation of the Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming. The invention further relates to the method for management of plant growth or crops by using Bio-Nitrogen fertilizer formulation with full of life for better crop productivity and sustainable agriculture along with increasing the crop nutrition, crop productivity and soil fertility.

Claims

1. A Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming comprising 15 to 25% by weight combination of nitrogen fixing microorganisms, cultures 20-30% by weight of nitrogen rich carrier materials, 20 to 30% by weight of bentonite along with nutrients, minerals and micronutrient minerals.

2. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the nitrogen fixing microorganisms are selected from group of independently fixing the nitrogen through biological nitrogen fixer which is Azotobacter sp., associative nitrogen fixing with biological association with crop which is Azospirillum sp., symbiotic nitrogen fixing with host crop plant which is Rhizobium sp. and endophytic nitrogen fixer which is Gluconacetobater diazotrophicus.

3. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the amount of combination of nitrogen fixing microorganisms is 15-25% by weight of total formulation at the ratio of each microorganism in the range of 10 to 40% (w/w).

4. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the nitrogen rich carrier materials selected is legume or alfalfa crop residue, amino acids and protein hydrolysates, fish meals.

5. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the amount of nitrogen rich carrier materials is 20-30% by weight of total formulation.

6. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the amount of bentonite is 20-30% by weight of total formulation.

7. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the nutrients are selected from amino acids, protein hydrolysates, carbohydrates and salts.

8. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 7 wherein the amount of amino acids, protein, carbohydrates and salts is 4-5% by weight of total formulation.

9. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the minerals are selected from Ca, P, Cl, B and Zn at a concentration of 5-10% by weight of total formulation.

10. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the elements essential for biological nitrogen fixation are selected from Cu, Mo, Fe, Co and Ni at a concentration ranging from 5-500 PPM by weight of total formulation.

11. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 1 wherein the formulation is in the form of granular form or powder form or liquid form with partially or fully soluble in water.

12. The Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 11 wherein the formulation is in the granular form.

13. A process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming comprising: a. mixing of the 40-50% by weight of nitrogen rich carrier material, 20 to 30% by weight of bentonite along with nutrients, minerals and micronutrient minerals by blending them properly to form the uniform mixture; b. then to the above mixture at step (a) is mixed with 15 to 25% by weight of culture of combination of nitrogen fixing microorganisms; c. then the mixture formed in the step (b) is subjected for granulation to form the granules; d. then the granules formed at step (c) are subjected for drying at low temperature; and e. after that, the granules are dried to form as Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming.

14. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the amount of culture of combination of nitrogen fixing microorganisms is 15% by weight of total formulation at the ratio of each microorganism in the range of 10 to 40% (w/w).

15. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the nitrogen fixing microorganisms are selected from group of independently fixing the nitrogen through biological nitrogen fixer which is Azotobacter sp., associative nitrogen fixing with biological association with crop which is Azospirillum sp., symbiotic nitrogen fixing with host crop plant which is Rhizobium sp. and endophytic nitrogen fixer which is Gluconacetobater diazotrophicus.

16. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the amount of nitrogen rich carrier material is 20-30% by weight of total formulation.

17. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the amount of bentonite is 20-30% by weight of total formulation.

18. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the nutrients are selected from amino acids, carbohydrates, protein hydrolysate and salts at the concentration of 4-5% by weight of total formulation.

19. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the amount of micronutrients is 5-10% by weight of total formulation.

20. The process for preparation of Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming as claimed in claim 13 wherein the amount of elements concentration ranging from 5-500 PPM by weight of total formulation.

Description

BRIEF DESCRIPTION OF THE DRAWINGS/FIGURES

[0046] FIG. 1 Effect of different Bio-Nitrogen fertilizer formulation on growth attributes of Paddy crop

[0047] FIG. 2 Effect of different Bio-Nitrogen fertilizer formulation on yield attributes of Paddy crop

DETAILED DESCRIPTION OF THE INVENTION

[0048] The present invention relates to the Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming. It particularly relates to the Bio-Nitrogen fertilizer formulation which reduces the use of chemical fertilizer and increase nutrients use efficiency and nitrogen bio-availability for better crop productivity and sustainable agriculture along with increasing the crop nutrition, crop productivity and soil fertility. It specifically relates to the Bio-Nitrogen fertilizer formulation containing nitrogen fixers from multiple sources such as Azotobacter sp., Azospirillum sp., Rhizobium sp., and Gluconacetobater diazotrophicus. The invention also relates to the process for preparation of the Bio-Nitrogen fertilizer formulation to fix the nitrogen from multiple sources for organic and sustainable farming. The invention further relates to the method for management of plant growth or crops by using Bio-Nitrogen fertilizer formulation with full of life for better crop productivity and sustainable agriculture along with increasing the crop nutrition, crop productivity and soil fertility. [0049] 1. Formulation containing multiple nitrogen fixing organisms with compatibility and functionality to provide nitrogen by. [0050] Independently fixing the nitrogen through biological nitrogen fixer such as Azotobacter sp. [0051] Associative nitrogen fixing with biological association with crop such as Azospirillum sp. [0052] Symbiotic nitrogen fixing with host crop plant such as Rhizobium sp. [0053] Endophytic nitrogen fixer such as Gluconacetobater diazotrophicus. [0054] 2. Formulation enriched with nitrogen fixing essential micro and trace elements such as Cu, Mo, Fe, Co and Ni to enhance the establishment and performance of the biological nitrogen fixation system. [0055] 3. Formulation enriched with naturally occurring minerals aiding in biological nitrogen fixation such as Ca, P, Cl, B and Zn as part of the carrier material to enhance to nitrogen fixing efficacy and nitrogen bio-availability. [0056] 4. Formulation containing pre-biotic media for the nitrogen fixing above said microbes. [0057] 5. Formulation fortified with nitrogen rich carrier materials such as legume or more specifically alfalfa crop residue, amino acids and protein hydrolysates, fish meals etc. [0058] 6. Formulation containing soluble or partially soluble organic carrier material to hold and sustain the formulation for longer period of self-life. [0059] 7. Formulation either in granular, powder or liquid forms either partially or fully soluble in water.

Bio-Nitrogen: Preparation Details (Instance of Artwork/Formulation in Use).

Working Example

Raw Material:

[0060] 1. Bio-fertilizer liquid formulation: (prepared with CFU of 5×10{circumflex over ( )}9): 15 to 25% (w/w) [0061] a) Azotobacter sp. preferably Azotobacter chroococcum [0062] b) Azospirillum sp. preferably Azospirillum brasilense [0063] c) Rhizobium sp. preferably Rhizobium leguminosarum [0064] d) Gluconacetobater diazotrophicus [0065] All above microbe will be mixed in the range 10 to 40% of each culture. [0066] 2. Media components (amino acids, Protein hydrolysate, carbohydrates, Salt): 4-5% (w/w) [0067] The media components in the proportion of: [0068] Amino acids: 20-30% [0069] Protein hydrolysate: 30-50% [0070] Salt: 20-30% [0071] 3. Nitrogen rich carrier materials such as legume or more specifically alfalfa crop residue, amino acids and protein hydrolysates, fish meals: 20-30% (w/w) [0072] legume or more specifically alfalfa crop residue: 30-40% [0073] Amino acids and protein hydrolysates: 10-20% [0074] Fish meals: 10-20% [0075] 4. Carrier material along with natural occurring minerals (Ca, P, Cl, B and Zn): 5-10% (w/w) [0076] Ca: 25-50% [0077] P: 25-50% [0078] Cl: 1-5% [0079] Zn: 5-10% [0080] B: 1-5% [0081] 5. Nitrogen fixing essential micro and trace elements (Cu, Mo, Fe, Co and Ni): 2-3% (w/w) [0082] Nitrogen fixation aiding elements such as Fe, Cu, Mo, Co and Ni in the range which enhances the process of biological nitrogen fixation by enhancing the enzyme activity which are critical to the vital process of diazotrophic micro-organisms. Composition of each of the elements shall be as in the table of the final formulation.

TABLE-US-00001 TABLE 1 Nitrogen fixing essential micro and trace elements Sl. No. Element Composition (in PPM) 1 Fe 400 to 500 ppm 2 Cu 200 to 250 ppm 3 Mo 25 to 50 ppm 4 Co 20 to 30 ppm 5 Ni 5 to 10 ppm [0083] 6. Bentonite: 20-30% (w/w)

Procedure:

[0084] 1. Mix nitrogen rich carrier material, media component, mineral matters and bentonite, according to the proposition mentioned in the composition and blend it properly for 15 minutes. [0085] 2. Then above mixture is mixed with the 15-25% biofertilizer liquid formulation to achieve optimal condition for granulation process. [0086] 3. The mixed powder is fed into granulator. [0087] 4. During process of granulation, spray 1 to 5% of biofertilizer liquid formulation for proper granulation. [0088] 5. Dry the granules low temperature (40-60 degree centigrade), until it dries completely. Keep the CFU to the >95% safe, live and efficacious in the process of drying. [0089] 6. Dried granules are sieved and packed in various size bags.

TABLE-US-00002 TABLE 2 Effect of different Bio-Nitrogen fertilizer formulation on growth attributes of Paddy crop % Number % Increase Plant of Increase in number Treatment height tillers/ Plant of tillers/ code Treatment details Dosage (cm) hill height hill T1 Bio-fertilizer: Azospirillum sp 10 103.00 11.00 9.71 9.09 kg/acre T2 Bio-fertilizer Consortia (Bio-N, Bio-P 10 106.00 13.00 12.26 23.08 and Bio-K) kg/acre T3 Bio-Nitrogen fertilizer formulation 10 108.00 15.00 13.89 33.33 (Azotobacter sp., + Azospirillum sp., + kg/acre Rhizobium sp., + Gluconacetobater diazotrophicus + Media components + Nitrogen rich carrier material + Plant micro-nutrient mineral media) T4 Control 93.00 10.00 0.00 0.00

TABLE-US-00003 TABLE 3 Effect of different Bio-Nitrogen fertilizer formulation on yield attributes of Paddy crop Grain % % yield Straw Increase Increase Treatment (Qt/ yield in Grain in Straw code Treatment details Dosage acre (Qt/acre) yield yield T1 Bio-fertilizer: Azospirillum sp 10 23.78 32.62 15.05 7.73 kg/acre T2 Bio-fertilizer Consortia (Bio-N, Bio- 10 25.36 34.00 20.35 11.47 P and Bio-K) kg/acre T3 Bio-Nitrogen fertilizer formulation 10 27.00 35.90 25.19 16.16 (Azotobacter sp., + Azospirillum sp., + kg/acre Rhizobium sp., + Gluconacetobater diazotrophicus + Media components + Nitrogen rich carrier material + Plant micro-nutrient mineral media) T4 Control 20.20 30.10 0.00 0.00