DEVICE FOR SEPARATING REGENERATIVE AND ADULT STEM CELLS

Abstract

This disclosure relates to a device for separating regenerative and/or adult stem cells from a fatty tissue taken from a biological structure. In one embodiment, the device includes a container for holding a substance mixture, the substance mixture including the fatty tissue and the regenerative and/or adult stem cells, and the container including at least two chambers separated from each other by at least one membrane. The device additionally includes a substance mixture feeding facility configured to introduce the substance mixture into the container, a rinsing agent feeding facility configured to introduce a fluid into the container, a stem cell removal facility configured to remove separated stem cells from the container, a rinsing agent removal facility configured to remove a substance mixture from the container that has been at least partially depleted of regenerative and/or adult stem cells, and a specifically permeable membrane.

Claims

1. A device for separating one or more of regenerative cells, adult stem cells, or a combination thereof from a fatty tissue taken from a biological structure, comprising: a container for holding a substance mixture, the substance mixture comprising the fatty tissue and the one or more of regenerative cells, adult stem cells or a combination thereof, wherein the container comprises at least two chambers separated from each other by at least one membrane; a substance mixture feeding facility configured to introduce the substance mixture into the container; at least one rinsing agent feeding facility configured to introduce a fluid into the container; a stem cell removal facility configured to remove the one or more of regenerative cells, adult stem cells, or a combination thereof from the container, and wherein the stem cell removal facility is separated from the substance mixture feeding facility by the at least one membrane; at least one rinsing agent removal facility configured to remove the substance mixture from the container, the substance mixture being reduced by at least a portion of the one or more of regenerative cells, adult stem cells, or a combination thereof; and at least one specifically permeable membrane, wherein the specifically permeable membrane is permeable for a specific part of the substance mixture.

2. The device according to claim 11, wherein the means for separating the substance mixture comprise a tube with a pressure compensation connection to a first chamber and a suction element connected to an underpressure source, said suction element being arranged in the tube.

3. The device according to claim 1, wherein at least one chamber of the at least two chambers comprises means for temperature adjustment.

4. The device according to claim 3, wherein the means for temperature adjustment comprise at least one temperature sensor and at least one of a heating sleeve arranged outside of the heating chamber or a heating element arranged within the chamber.

5. The device according to claim 1, further comprising a means for generating one or more of an at least partially turbulent flow and an at least partially laminar flow into the substance mixture and rinsing agent, wherein said means for generating one or more of an at least partially turbulent flow and an at least partially laminar flow are provided in an area of a membrane positioned closest to the stem cell removal device.

6. The device according to claim 5, wherein said means for generating one or more of an at least partially turbulent flow and an at least partially laminar flow comprise a stirring element or a nozzle for generating a fluid jet.

7. The device according to claim 5, wherein said means for generating one or more of an at least partially turbulent flow and an at least partially laminar flow comprise a remotely-powered electromechanical stirring element.

8. The device according to claim 1, wherein the rinsing agent removal facility is configured to again introduce a rinsing agent independently of the substance mixture that has been reduced by the at least a portion of the one or more of regenerative cells, adult stem cells, or a combination thereof and to remove it again.

9. The device according to claim 1, wherein the device at least partially consists of sterile components.

10. A system for separating one or more of regenerative cells, adult stem cells, or a combination thereof from a fatty tissue taken from a biological structure, comprising: the device according to claim 1; and a central control appliance, wherein the central control appliance is connected via a mechanical interface for the device to said device as a basis assembly, and wherein the central control appliance is configured to control or regulate one or more of an underpressure source, a temperature adjustment, a means for generating a flow, a rinsing agent conveyance facility, or combinations thereof.

11. The device according to claim 1, wherein a first chamber of the at least two chambers of the container comprises means for separating the substance mixture and a fluid combined with the substance mixture.

Description

[0028] The invention will now be explained in greater detail with reference to an exemplary embodiment and related drawings, in which:

[0029] FIG. 1 shows a schematic profile view of a device according to the invention in a preferred embodiment;

[0030] FIG. 2 shows a schematic depiction of a detailed profile view of an area of an exemplary device according to the invention with an electromechanical stirring element in a preferred embodiment, and

[0031] FIG. 3 shows a schematic depiction of a profile view of a system according to the invention in a preferred embodiment.

[0032] FIG. 1 shows a schematic profile view of a device according to the invention in a preferred embodiment. The device according to the invention for separating regenerative and/or adult stem cells 10 comprises a container for holding a substance mixture 20. The container 20 comprises a first chamber 30, a second chamber 40, a third chamber 50 and a fourth chamber 60. The third chamber 50 is separated from the fourth chamber 60 by a radial cylinder 70. The radial cylinder 70 comprises a filter membrane, which is designed to withhold the stem cells. The first chamber 30 is separated from the second chamber 40 and the second chamber 40 is also separated from the third chamber 50 by filter membrane 80. The first chamber 30 comprises a substance mixture feeding facility 90. The first chamber 30 further comprises a rinsing agent feeding facility 100. A schematically indicated stem cell removal facility 110 is connected to the fourth chamber 60. Further, a schematically indicated rinsing agent removal facility 120 is connected to the fourth chamber 60. The first chamber 30 of the device 10 has means for separating the substance mixture and a fluid combined with the substance mixture which can be systematically assigned to the substance mixture feeding facility 90. The means 130 comprise a tube 140 with a tube 140 with a pressure compensation connection 150 to the first chamber 30.

[0033] Further, the means 130 comprise a suction element 160 which is arranged in the tube 140 and which is connected on a suction side 170 with an underpressure source not shown here. The device 10 further comprises means for temperature adjustment 180. The means 180 comprise a heating element 190, a heating sleeve 200 and a plurality of temperature sensors 210.

[0034] The heating sleeve 200 is affixed outside on the first chamber 30. In other exemplary embodiments, further heating sleeves 200 can be affixed outside on further chambers. The heating element 190 is in this exemplary embodiment integrated into a mixing element 220. Equally, the temperature sensors 210 are integrated in the mixing element 220. In other exemplary embodiments, the arrangement and number of the heating elements 190 and temperature sensors 210 can vary. A person skilled in the art makes these adaptations depending on the process requirements. The radial cylinder 70 comprises a membrane 230 which is closest to the stem cell removal facility 110. In the area of the closest membrane 230, means are provided for generating an at least partially turbulent and/or laminar flow 240. These comprise a remotely-powered electromechanical stirring element 250, which is described in greater detail in FIG. 2. Further, the means 240 comprise an electromagnet 290 for generating an electromagnetic alternating field, which is not described further here and which is schematically indicated in FIG. 2. The rinsing agent removal facility 120 is also designed to again introduce a rinsing agent independently of the substance mixture reduced by adult stem cells and to remove it again. In the upper portion of the device 10, a connection section 260 is provided via which, for example, cables for e.g. the heating element 190 and the temperature sensors 210 can be connected and guided. Further, in this area, a connection 270 is provided for applying an underpressure. In those areas which come into contact with the substance mixture, the device 10 consists of sterile components. These components include e.g. the first chamber 30, the second chamber 40, the third chamber 50, the fourth chamber 60, the substance mixture feeding facility 90, the means for separating the substance mixture and a fluid 130 combined with the substance mixture, the mixing element 220, the filter membrane 80, the radial cylinder 70, the rinsing agent removal facility 120, the stem cell removal facility 110 and the remotely-powered electromechanical stirring element 250, not shown here. The latter is arranged in area A and is described in greater detail in FIG. 2. Area A is arranged in a lower area 280 of the radial cylinder 70. The remotely-powered electromechanical stirring element 250 has a capsular form in this exemplary embodiment. The electromagnet 290 is arranged below the remotely-powered stirring element 250.

[0035] FIG. 2 shows a schematic depiction of a detailed profile view of a partial area of a device according to the invention in a preferred embodiment. The same reference numerals apply as for FIG. 1, so that for the features referenced with these numerals, the same applies as described in FIG. 1. The partial area described is a partial area described in FIG. 1 for separating regenerative and adult stem cells 10. The partial area comprises e.g. a third chamber 50 and a fourth chamber 60, an area A and a remotely-powered electromechanical stirring element 250 and the electromagnet 290. A volume of the third chamber 50 is largely filled by a core element for volume reduction 55, so that only a small gap remains free between the core element for volume reduction 55 and the closest membrane 230. The electromagnet 290 is arranged below the remotely-powered electromechanical stirring element 250 and is designed to generate an electric alternating field in order to move the remotely-powered electromechanical stirring element 250. Thus, within the radial cylinder 70, a partially turbulent and partially laminar flow can be set. In other exemplary embodiments, purely turbulent or purely laminar flows can also be generated, e.g. with a nozzle.

[0036] FIG. 3 shows a schematic depiction of a profile view of a system according to the invention in a preferred embodiment. The same reference numerals apply as for FIG. 1, so that for the features referenced with these numerals, the same applies as described in FIG. 1. FIG. 3 shows the device for separating regenerative and adult stem cells 10 in connection with a central control appliance 300. The device for separating regenerative and adult stem cells 10 is described in FIG. 3a. The central control appliance 300 is described in FIG. 3b. The central control appliance 300 and the device 10 have a mechanical interface 310, so that the device 10 can be arranged on the central control appliance 300. The latter has a plurality of connection and operating elements 320. The central control appliance 300 is designed to regulate an underpressure source, a temperature adjustment, means for generating a flow in the form of a mechanical stirring element 250 remotely powered by an electromagnet, and a rinsing agent conveyance facility. The central control appliance 300 has e.g. connections for media 330 and connections for power and signals 340.

LIST OF REFERENCE NUMERALS

[0037] 10 Device for separating regenerative and/or adult stem cells [0038] 20 Container for holding a substance mixture [0039] 30 First chamber [0040] 40 Second chamber [0041] 50 Third chamber [0042] 55 Core element for volume reduction [0043] 60 Fourth chamber [0044] 70 Radial cylinder [0045] 80 Filter membranes [0046] 90 Substance mixture feeding facility [0047] 100 Rinsing agent feeding facility [0048] 110 Stem cell removal facility [0049] 120 Rinsing agent removal facility [0050] 130 Means for separating the substance mixture and a fluid combined with the substance mixture [0051] 140 Tubes [0052] 150 Pressure compensation connection [0053] 160 Suction element [0054] 170 Suction side [0055] 180 Means for temperature adjustment [0056] 190 Heating element [0057] 200 Heating sleeve [0058] 210 Temperature sensors [0059] 220 Mixing element [0060] 230 Closest membrane [0061] 240 Means for generating an at least partially turbulent and/or laminar flow [0062] 250 Remotely-powered electromechanical stirring element [0063] 260 Connection section [0064] 270 Connection [0065] 280 Lower area [0066] 290 Electromagnet [0067] 300 Central control appliance [0068] 310 Mechanical interface [0069] 320 Connection and operating elements [0070] 330 Connections for media [0071] 340 Connections for power and signals [0072] A Area