Sample collection and processing device

Abstract

A device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).

Claims

1. A device for separating and concentrating target particles or molecules from a fibrinogen-containing liquid sample, the device comprising: a container comprising an open top end adapted for receiving a removable closure cap, a closed bottom end, and an interior wall, the top end, the bottom end, and the interior wall defining a reservoir portion for receiving the sample; thrombin or a thrombin-like enzyme in the reservoir; an anchor element configured for nucleating a polymerized fibrin pellet comprising the target particles or molecules trapped therein; and a removable closure cap comprising an elongated pin element; wherein: a) the anchor element is located at or toward the end of the elongated pin element; or b) the anchor element is integrated into the bottom end or interior wall.

2. The device of claim 1, wherein the anchor element is in contact with the reservoir during operation of the device.

3. The device of claim 1, wherein the thrombin or the thrombin-like enzyme is in a lyophilized form.

4. The device of claim 1, wherein the bottom end of the container comprises an elongated pin element extending into the reservoir, and wherein the anchor element is located at or towards the end of the pin element.

5. The device of claim 1, wherein the anchor element has a hook or ring shape.

6. The device of claim 1, wherein the volume of the container is between 0.1 to 20 ml.

7. A method for separating and concentrating target particles or molecules from a sample containing fibrinogen and target particles, the method comprising: (a) providing the device of claim 1; (b) adding the sample to the container; (c) contacting fibrinogen in the sample with the thrombin or thrombin-like enzyme, thereby converting the fibrinogen within the sample at least partially into fibrin, thereby nucleating a polymerized fibrin pellet on the anchor element and encompassing the target particles or molecules within the pellet; and (d) separating the polymerized fibrin pellet from a remainder of the sample.

8. The method of claim 7, wherein the device further comprises a removable closure cap comprising the anchor element integrated therein, and the separation step (d) comprises removing the removable closure cap thereby removing the anchor element and polymerized fibrin pellet from the reservoir.

9. The method of claim 7, wherein the anchor element is integrated into the container, and the separation step (d) comprises decanting a portion of the sample from the container.

10. The method of claim 7, wherein the method further comprises a recovery step comprising resuspending the polymerized fibrin pellet in a liquid medium that allows lysis of the polymerized fibrin pellet, thereby recovering the target molecules or particles.

11. The method of claim 10, wherein the device further comprises a removable closure cap comprising the anchor element integrated therein, and the recovery step comprises immersing the removable closure cap in a resuspension container containing a resuspending liquid medium.

12. The method of claim 10, wherein the anchor element is integrated into the container, and the recovery step comprises adding a resuspending liquid medium to the container.

13. The method of claim 7, wherein a size of the polymerized fibrin pellet is at most 1/10 of an initial volume of the sample.

14. The method of claim 7, wherein the target molecules or particles comprise bacteria, virus, yeast, cells, proteins, peptides or nucleic acids.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The objects and features of the present invention are set forth with particularity in the appended claims. The present invention, both as to its organization and manner of operation, together with further objects and advantages, may best be understood by reference to the following description, taken in connection with the accompanying drawings, wherein

(2) FIG. 1 is a schematic representation of a preferred embodiment of the device according to the invention,

(3) FIG. 2(a) and FIG. 2(b) are schematic representations of operation of the device,

(4) FIG. 3 illustrates separation and concentration of target particles or molecules,

(5) FIG. 4 illustrates a secondary container for target recovery and suspension,

(6) FIG. 5 shows three different types of anchor element,

(7) FIG. 6 is a diagram of overall operation, and

(8) FIGS. 7 (a), (b) and (c) show a further embodiment of the device in three different states.

(9) FIG. 1 is a schematic representation of a preferred embodiment of the device for separating and concentrating target particles or molecules out a sample of liquid comprising a container (1) for collecting the sample and a closing cap (2) positioned on the open first top side of the container. The container further comprises a second closed bottom end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The closing cap (2) comprises an elongated downwardly-protruding pin element (3) immersed in the sample in the device's reservoir (5) and comprising at its lower end an anchor element (4).

(10) FIG. 2 is a schematic representation of the device operation for the separation and concentration of target particles or molecules from a sample. As shown in FIG. 2 (a) the device according to the invention is designed to receive a liquid sample that may include target particles or molecules (8) to be separated or concentrated. The sample may include fibrinogen as a native component (e.g. blood). In the case of a fibrinogen-free sample to be processed, the container (1) should include fibrinogen as an additive to be mixed with the fibrinogen-free sample. FIG. 2 (b) shows that as the sample is added to the device, the fibrinogen within the liquid (either native from the sample or not) will be converted at least partially to fibrin upon subjecting the sample to thrombin or thrombin-like enzymes, thereby forming a fibrin network that will trap the target molecules or particles. The so-formed fibrin network will retract to form a polymerized fibrin pellet (9) hooked or adhered around the anchor element (4).

(11) FIG. 3 is a schematic representation of the device operation for the separation and concentration of target particles or molecules from a sample. Upon retraction of the fibrin network into a polymerized fibrin pellet (9) hooked around the anchor element (4), the closure cap (2), with the anchor element (4) and the hooked polymerized fibrin pellet (9), will be removed from the container (1), thereby separating the polymerized fibrin pellet (9), with the trapped targets, from the sample medium. In a subsequent step, the closure cap (2) can be immersed in a secondary container (6) containing resuspending liquid medium (7) that will lyse the fibrin pellet, thereby allowing recovery of the target molecules or particles (8). Secondary container (6) is preferably smaller than the device according to the present invention.

(12) FIG. 4 is a schematic representation of a preferred embodiment of the target recovery and resuspension device comprising a smaller secondary container (6) for containing the resuspending liquid. The container (6) comprises an open first top end that is adapted to receive a cap, such as the closure cap (2), comprising anchor element (4) of the device according to the invention. The container (6) further comprises a second closed bottom end and at least one interior wall defining a reservoir portion (7) for containing the resuspension liquid that allows to lyse the fibrin pellet and thereby recover the targets (8). Target recovery is achieved by immersing anchor element (4) of closure cap (2) into the recovery container (6).

(13) FIG. 5 shows different embodiments of the anchor element (4): namely (4a) ring shape, (4b) chemical or biological coating and (4c) irregular hook shape.

(14) FIG. 6 is a schematic representation of overall operation of the method for separating and concentrating target particles or molecules from a fibrinogen-containing sample using the device according to the invention. In step 1, sample is added to the device's container. In step 2 there is a fibrin network formation reaction. In step 3, the fibrin pellet retracts around a hydrophobic pin (anchor element) fitted on the tube cap. Step 4 is a pellet separation phase. In step 5 the pellet is immersed in a small volume of resuspension solution. Finally, in step 8, the target particles or molecules are recovered.

(15) FIG. 7 is a schematic representation of a preferred embodiment of the device for separating and concentrating target particles or molecules from a liquid sample comprising, differentiated with respect to the device of FIG. 1, an upwardly-extending elongated pin element (3) immersed in the reservoir portion (5) and positioned in the closed bottom end of the container (1). The elongated pin element (3) further comprises at its upper end an anchor element (4). In operation, a retracted fibrin network forming a polymerized fibrin pellet (9) will be hooked or adhere around the anchor element (4). The so-formed fibrin pellet can be thereafter separated by decanting the surrounding liquid medium. Target recovery is achieved by adding, to the same container (1), a fibrin pellet lysis buffer (7) that will lyse the fibrin pellet allowing thereby the recovery of the target molecules or particles (8).

DETAILED DESCRIPTION OF THE INVENTION

(16) As illustrated in FIG. 1, a device according to the invention for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises: a container (1) for collecting the sample and a closure (2). The container (1) comprises a first top end and a second bottom end and at least one interior wall defining a reservoir portion (5) for receiving the sample, wherein the reservoir portion comprises at least one anchor element (4) to locally hook the polymerized fibrin pellet (9) formed upon addition of the sample to the container and wherein target particles or molecules are trapped within the polymerized fibrin pellet.

(17) The invention also covers a method for collecting and processing biological samples comprising: providing a container comprising thrombin and potentially fibrinogen in appropriate concentration to lead to effective separation of target molecules or particles from a surrounding complex liquid medium. This method further allows to recover the target(s) that are highly concentrated within the polymerized material and that preferably have a volume that is at most 1/10 of the initial sample volume. Furthermore, an advantage of the disclosed method is the capability to reach a concentration rate of 1/100 to 1/1000 of the initial sample volume. The so-concentrated target(s) can be thereafter processed very easily through further purification step(s) and/or directly analyzed using state-of-art methodologies.

(18) One major advantage of the disclosed device is to allow an automatic and easy separation of the target particles or molecules by integrating into the device an anchor element (4) that will locally hook, adhere or nucleate the polymerized fibrin pellet. In practice, the anchor element (4) somehow constitutes a “nucleation” point around which the fibrin pellet will be formed and retracted into a small pellet as shown in FIG. 2. In a preferred embodiment, the anchor element (4) is integrated with the closure cap (2) that comprises an elongated pin element (3) to be immersed in the liquid sample contained in the reservoir portion (5) upon closing the cap. In this configuration, the anchor element (4) is located at or towards the end side of the pin element (3).

(19) Integration of the anchor element (4) in the cap has an advantage of allowing an easy separation of the polymerized fibrin pellet (9) hooked around the anchor element (4). As shown in FIG. 3, the closure cap (2) containing anchor element (4) with the hooked polymerized fibrin pellet (9) can be removed from the container (1), thereby achieving the separation of concentrated polymerized fibrin pellet (9), with the targets trapped in it, from the initial sample medium. The closure cap (2) can thereafter be plugged in a smaller container (6) containing resuspending liquid medium (7) for the target recovery, as shown in FIG. 4.

(20) Although the instant invention has been illustrated by a preferred embodiment where the anchor element (4) is integrated in the closure cap, in practice the anchor element can also be part of the device container (1) as for instance integrated into the walls or the bottom sides of the reservoir portion (as shown in FIG. 7). In this case, the separation process will be achieved by decanting the sample from the device container (1) while keeping the fibrin pellet attached (or hooked) to the anchor element (4). The target recovery is achieved by adding, to the same container (1), a fibrin pellet lysis buffer (7) (as shown in FIG. 7 c), that will lyse the fibrin pellet allowing thereby the recovery of the target molecules or particles (8).

(21) The anchor element according to the invention can be any local part with irregular or regular shape like the ones shown in FIG. 5. In preferred embodiments, the anchor element can be a local surface coating that allows local attachment of the fibrin pellet. Non-limiting examples of such coating include chemical coating (e.g hydrophobic coating) and biological coating (e.g. fibrinogen/fibrin binding molecules such as thrombin, clotting factor XIII, bacterial fibrinogen binding proteins and tissue plasminogen activator (t-PA)).

(22) The device as disclosed herein can encompass any sample collection device including tubes such as test tubes and centrifuge tubes; closed system sample collection devices, such as collection bags; syringes, especially pre-filled syringes; catheters; microwells and other multi-well plates; arrays; tubing; laboratory vessels such as flasks, and assemblies; pipettes and pipette tips, etc. In general, the instant invention concerns any container suitable for holding a biological sample, as well as containers and elements involved in transferring samples provided they meet the criteria outlined herein.

(23) Based on the foregoing disclosure, the present invention further includes a method for separating target molecules or particles from a sample using a sample collection device that can be very easily used manually or integrated with state-of-the-art automated systems which makes this sample preparation method easily integrated in routine laboratory work flows as shown in FIG. 6.

(24) The volume of the sample container is between 0.1 to 100 ml and preferably between 0.1 to 10 ml. The concentration of fibrinogen in the sample is preferably at least 0.1 μg/ml. In a preferred embodiment the concentration of fibrinogen in the sample is between 0.1 to 100 mg/ml and most preferably between 10 mg/ml to 10 μg/ml.

(25) The device may further include as an additive a thrombin or thrombin enzyme. The thrombin concentration is 0.01 to 10 I.U./ml and preferably within the range of 0.1 to 2 I.U./ml of sample. In practice, the quantity of the thrombin or thrombin like enzyme must be adjusted in correspondence to the fibrinogen concentration within the device to obtain the desired fibrin network structure and pellet size. The thrombin amount is preferably less than 20 I.U. thrombin per mg of fibrinogen, preferably in a range between 0.01 to 10 I.U. thrombin per mg of fibrinogen, more preferably between 0.1 to 1 I.U. thrombin per mg of fibrinogen.

(26) In case of blood samples such as whole blood, the sample collection device according to the invention can further include coagulation agents that promote the generation of endogenous thrombin within the sample. Such promoting agents can be for instance selected from groups comprising powdered or fibrous silicate compounds such as kaolin, Celite, diatomaceous silica and glass fibers, fine powders of calcium compounds such as calcium carbonate and calcium sulfate, thrombin-like substances derived from snake venoms, and polyphenols that can activate blood pelleting factors to promote coagulation. Further, these coagulation promoting agents can be, for example, added individually or in combination into the sample or coated inside the wall of sample container. The amount of such promoting agents must be adjusted in a way to control the coagulation process and obtain a small fibrin pellet size.

(27) In a preferred embodiment to control the fibrin network structure in order to trap target molecules or particles from a sample, the sample collection device according to the invention can further include additives that allow to adjust the concentration of calcium. In practice, this can be achieved by adding a calcium ion source to the device. The calcium ion source is preferably Calcium Chloride (CaCl.sub.2), preferably in a concentration range between 1 to 10 mg per ml of sample volume, even more preferably between 4 to 7 mg per ml of sample volume, most preferably between 5 to 6 mg per ml of sample volume. In blood samples, for instance, calcium is naturally present and the adjustment of the calcium concentration can be achieved by further adding to the device calcium chelating agents such as GDTA, EDTA or citrate.

(28) Further, the device according to the invention may include additives comprising molecules having: (I) fibrin/fibrinogen-binding moiety and (II) a substance-capturing moiety directed against the target molecules or particles. Accordingly, the substance-capturing moiety directed against the target molecules or particles can be selected from the group comprising antibodies, nucleic acids and aptamers designed to specifically recognize the said target molecules or particles. Further, the substance-capturing moiety can be coupled or combined with a fibrin/fibrinogen-binding moiety selected from the group comprising thrombin, fibronectin, bacterial fibrinogen binding proteins, tissue-type plasminogen activator, integrines and moieties derived from any member of this group. In a preferred embodiment, the fibrin/fibrinogen-binding moiety and the substance-capturing moiety are combined in a fusion molecule.

(29) Further, the device according to the invention can include additives comprising a fibrinogen recombinant protein. Such recombinant fibrinogen protein can be specifically designed to enhance or inhibit affinity interactions of the recombinant fibrinogen protein with specific target molecules or particles contained in the sample under use within the device. In a preferred embodiment, the recombinant protein in use within the device is a fibrinogen fusion protein with a capturing moiety domain directed against the said target molecules or particles. In another embodiment, the fibrinogen fusion protein further includes a degradation site. This will be particular useful for recovering the bound target molecules or particles from the fibrin network during a lysis step. In a preferred embodiment, the degradation site is an enzymatic or hydrolytic degradation site. In a most preferred embodiment, the degradation site is an enzymatic degradation site, which is cleaved by an enzyme selected from the group consisting of plasmin and matrix metalloproteinase.

(30) In practice all of the previously described additives can be added to the sample after the sample collection or already integrated within the device. In the last case, the additives can be integrated solubilized in an aqueous buffer solution. In a preferred embodiment, the said additives can be included within the device in a lyophilized form that can be solubilized just prior to the device use or upon the introduction of the sample into the device.

(31) The sample collection device according to the invention can be used to separate and concentrate target molecules or particles such as target cells, cell components, cell subpopulations (both eukaryotic and prokaryotic), bacteria, viruses, parasites, antigens, specific antibodies, toxins, proteins, nucleic acid sequences and the like.

(32) The sample collection device according to the invention can be used to separate and concentrate target molecules or particles from diverse samples. In general this includes whole blood, blood derivatives, blood components as well as fibrinogen-free samples (including but not limited to urine, sputum and swab). In this respect, the sample herein can refer to any sample type that needs to be tested, including food, clinical, environmental, and experimental samples.

(33) In practice, the device may also include an identification code. Such an identification code can be determined by, without limitation, a code bar or a color added to the device, or by the size and/or shape of the device itself. Such identification code can be used as a reference or indicator of the device's intended use and application. Devices according to several embodiments of the invention can be, in fact, differentiated according to their composition, sample type for which the device will be used and/or the target(s) that need to be separated.

(34) Those skilled in the art will appreciate that various adaptations and modifications of the just-described preferred embodiments can be configured without departing from the scope of the invention. Therefore, it is to be understood that, within the scope of the appended claims, the invention may be practiced otherwise than as specifically described herein.