BIO-PHOSPHATE FERTILIZER FORMULATION WITH FULL OF LIFE FOR CROP NUTRITION, CROP PRODUCTIVITY AND SOIL FERTILITY

Abstract

The present invention relates to the Bio-Phosphate fertilizer formulation for crop nutrition, crop productivity, and soil fertility. It particularly relates to the Bio-Phosphate fertilizer formulation which comprises phosphate solubilizing and plants growth-promoting microorganisms, rock phosphate, bentonite along with minerals, macro, and micronutrients. It specifically relates to the Bio-Phosphate fertilizer formulation containing Phosphate Solubilizing Microorganisms are selected from a combination of Phosphate Solubilizing bacteria selected from Bacillus spp., & Pseudomonas spp., and Phosphate Solubilizing fungi selected from Aspergillus spp., The invention further relates to the process for the preparation of Bio-Phosphate fertilizer formulation for sustainable agriculture along with increasing the crop nutrition, crop productivity, and soil fertility.

Claims

1. A Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility comprising 13 to 15% by weight of phosphate solubilizing and plant growth promoting microorganisms, 50 to 55% by weight of rock phosphate, 18 to 20% by weight of bentonite along with minerals, macro and micronutrients.

2. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the phosphate solubilizing microorganisms are selected from combination of Phosphate Solubilizing Bacteria selected from Bacillus spp & Pseudomonas spp and Phosphate Solubilizing fungi selected from Aspergillus spp.

3. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 2 wherein the amount of combination of Phosphate Solubilizing Bacteria and Phosphate Solubilizing fungi is 13-15% by weight of total formulation at the ratio of 1:1.

4. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 2 wherein the Phosphate Solubilizing Bacteria selected is Bacillus megaterium.

5. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 2 wherein the Phosphate Solubilizing fungi selected is Aspergillus spp.

6. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the Phosphate Solubilizing Bacteria and Plant growth promoting bacteria selected is Pseudomonas fluorescens.

7. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 6 wherein amount of the Pseudomonas fluorescens is 2-3% by weight of total formulation.

8. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the amount of rock phosphate is 50-55% by weight of total formulation.

9. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the amount of bentonite is 18-20% by weight of total formulation.

10. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the nutrients are selected from amino acids, protein, carbohydrates and salts.

11. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the amount of amino acids, protein, carbohydrates and salts is 3-5% by weight of total formulation.

12. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the amount of micronutrients and minerals is 2-3% by weight of total formulation.

13. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 1 wherein the formulation is in the form of granular form or powder form or liquid form with partially or fully soluble in water.

14. The Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 13 wherein the formulation is in the granular form.

15. A process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility comprising: a. mixing of the 50 to 55% by weight of rock phosphate, 18 to 20% by weight of bentonite along with minerals, macro and micronutrients by blending them properly to form the uniform mixture; b. then to the above mixture at step (a) is mixed with 13 to 15% by weight of culture of combination of Bacillus megaterium and Aspergillus spp; c. then the mixture formed in the step (b) is subjected for granulation to form the granules; d. then the granules formed at step (c) are subjected for drying at low temperature; and e. after that, the dried granules are coated with 2-3% by weight Pseudomonas fluorescens to form the coated granules as Bio-Phosphate fertilizer formulation.

16. The process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 15 wherein the amount of culture of combination of Bacillus megaterium and Aspergillus niger is 13-15% by weight of total formulation at the time of preparation combination of Bacillus megaterium and Aspergillus niger is 2-5% by weight of total formulation after drying the granule formulation.

17. The process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 15 wherein the amount of rock phosphate is 50-55% by weight of total formulation.

18. The process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 15 wherein the amount of bentonite is 18-20% by weight of total formulation.

19. The process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 15 wherein the nutrients are selected from amino acids, carbohydrates, protein and salts at the concentration of 3-5% by weight of total formulation.

20. The process for preparation of Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility as claimed in claim 15 wherein the amount of micronutrients and minerals is 2-3% by weight of total formulation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0052] The present disclosure relates to the Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility. It particularly relates to the Bio-Phosphate fertilizer formulation which reduces the use of chemical fertilizer and increase nutrients use efficiency and Phosphorus bio-availability for better crop productivity and sustainable agriculture. It specifically relates to the Bio-Phosphate fertilizer formulation containing phosphate in the form of P.sub.2O.sub.5, microbes especially phosphate solubilizers such as Bacillus spp, Pseudomonas spp, fungi in the form of spores Aspergillus spp and Trichoderma spp, complex media of Amino Acids, proteins, salts, minerals and Carbohydrates, plant growth promoting inputs such as humate and seaweed. The invention also relates to the process for preparation of the Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility. The invention further relates to the method for management of plant growth or crops by using Bio-Phosphate fertilizer formulation with full of life for crop nutrition, crop productivity and soil fertility.

[0053] Bio-Phosphate Preparation Details (Instance of Artwork/Formulation in Use).

WORKING EXAMPLE

[0054] Raw Materials: [0055] 1. Bio-fertilizer liquid formulation: (prepared with CFU of 5×10{circumflex over ( )}9): 13-15% (w/w) [0056] a) Bacillus megaterium (Phosphate Solubilizing Bacteria) —6.5-7.5% (w/w) [0057] b) Aspergillus niger (Phosphate Solubilizing fungi)—6.5-7.5% (w/w) [0058] 2. Rock Phosphate (ground into fine powder of size less than 0.1 mm) 50-55% (w/w) [0059] 3. Media components (Amino acid, Protein, Carbohydrates (Sucrose and Starch) and Salt): 3-5% (w/w) [0060] a. Amino acid and protein: Soya hydrolysate (2-3% (w/w)) [0061] b. Salt: Sodium chloride and Potassium chloride (1% (w/w) each) [0062] c. Carbohydrates-Jaggery 1-2% (w/w) [0063] 4. Plant micro-nutrient mineral media: 2-3% (w/w) [0064] a. Calcium carbonate: 1-2% (w/w) [0065] b. Sulphates of Zn, Mn and Fe: 0.5-1% (w/w) each [0066] c. Boron: 0.1-0.5% (w/w) [0067] 5. Bentonite: 18-20% (w/w) [0068] 6. Pseudomonas fluorescens (Phosphate Solubilizing Bacteria and Plant growth promoting bacteria) are prepared with CFU of 5×10{circumflex over ( )}9: 2-3% (w/w)

[0069] Procedure: [0070] 1. Mix 55% (w/w) Rock phosphate, 5% (w/w) media components, 2% (w/w) mineral matters and 20% (w/w) Bentonite and blend it properly for 15 minutes. [0071] 2. Then above mixture is mixed with the 10% (w/w) broth by checking the consistency of the moisture content required for moist formulation. [0072] 3. The mixed moist formulation is fed into granulator. [0073] 4. During process of granulation, spray remaining 5% (w/w) culture for proper granulation. [0074] 5. Dry the granules low temperature, until it dries completely. Keep the CFU to the >95% safe, live and efficacious in the process of drying. Dried granules are sieved and further pushed for the coating. [0075] 6. After drying process of granules, coat the granules with 2% (w/w) Pseudomonas fluorescens and coating agents. [0076] 7. Dried Granules are packed in various size bags. [0077] Formulation containing phosphate in the form of P.sub.2O.sub.5 in the range of 5% to 45%. [0078] Formulation comprising pre-biotic components in the form of complex media for microbes especially phosphate solubilizers: containing amino acids, proteins, salts, minerals and carbohydrates. [0079] Phosphate Solubilizing Bacteria in the form of cyst or endospore of various genera such as Bacillus spp, Pseudomonas spp. and various sp. [0080] Phosphate solubilizing Fungi in the form of spores Aspergillus spp. [0081] Phosphate Solubilizing Bacteria with multiple functionality such as phosphate solubilization, growth promoter and immunity building in the crop plant. [0082] Formulation enriched with various other essential minerals for optimal crop nutrition. [0083] Formulation enriched with plant growth promoting inputs such as humate, seaweed and amino acids. [0084] Formulation containing 1% to 15% organic carbon to enhance the microbial survivability and provide organic carbon to the crop/soil. [0085] Formulation either in granulation form or powder form or liquid form with partially or fully soluble in water.

TABLE-US-00001 TABLE 1 Effect of different ingredients of Bio phosphate on shoot and root growth of Maize crop 60 Days after sowing Shoot Root % % % % Shoot Root dry dry Increase Increase Increase Increase length length weight weight shoot Root shoot root Treatment Dosage (cm) (cm) (gm) (gm) length length weight weight T1 Bacillus 10 kg/acre 178.00 18.53 123.19 8.36 15.17 26.61 18.69 23.33 megaterium (Phosphate solubalizing bacteria) Solid granule T2 Aspergillus niger 10 kg/acre 179.23 18.36 121.36 8.01 15.75 25.93 17.47 19.98 (Phosphate solubalizing fungi) Solid granule T3 Pseudomonas 10 kg/acre 181.23 18.89 127.87 8.78 16.68 28.00 21.67 26.99 fluorescens (Phosphate solubalizing fungi) Solid granule T4 megaterium + Aspergillus 10 kg/acre 187.23 20.21 132.15 9.36 19.35 32.71 24.21 31.52 niger + Rock Phosphate + Media components (Amino acid, Protein, Starch and Salt) + Plant micro-nutrient mineral media + Pseudomonas fluorescens(Solid granule) T5 Rock Phosphate P2O5 (10.6%) 25 kg/acre 153.50 15.23 111.50 6.96 1.63 10.70 10.17 7.90 T6 Control 151.00 13.60 100.16 6.41 0.00 0.00 0.00 0.00 T1: Bacillus megaterium (Phosphate solubalizing bacteria) Solid granule, T2: Aspergillus niger (Phosphate solubalizing fungi) Solid granule, T3: Pseudomonas fluorescens (Phosphate solubalizing fungi) Solid granule, T4: Bacillus megaterium + Aspergillus niger + Rock Phosphate + Media components (Amino acid, Protein, Starch and Salt) + Plant micro-nutrient mineral media + Pseudomonas fluorescens(Solid granule), T5: Rock Phosphate P2O5 (10.6%) T6: Control

TABLE-US-00002 TABLE 2 Effect on growth & yield attributes of Maize Yield (kg acre.sup.−1) Concentration TDM % % % (cfu/gm (g Yield (kg acre.sup.−1) Increase Increase Increase Treatment and %) plant.sup.−1) Grain Stover TDM Grain Stover T1 Bacillus megaterium 10 kg/acre Grain Stover 7.67 10.01 12.98 (Phosphate solubalizing bacteria) Solid granule T2 Aspergillus niger 10 kg/acre 129.73 3062.00 3795.84 16.44 11.20 18.60 (Phosphate solubalizing fungi) Solid granule T3 Pseudomonas 10 kg/acre 135.06 3476.00 4009.20 19.74 21.77 22.93 fluorescens (Phosphate solubalizing fungi) Solid granule T4 Bacillus megaterium + 10 kg/acre 157.40 4349.60 4498.44 31.13 37.48 31.31 Aspergillus niger + Rock Phosphate + Media components (Amino acid, Protein, Starch and Salt) + Plant micro-nutrient mineral media + Pseudomonas fluorescens(Solid granule) T5 Rock Phosphate P2O5 (10.6%) 25 kg/acre 114.06 2912.80 3305.16 4.96 6.65 6.51 T6 Control 108.40 2719.20 3089.88 0.00 0.00 0.00 T1: Bacillus megaterium (Phosphate solubalizing bacteria) Solid granule, T2: Aspergillus niger (Phosphate solubalizing fungi) Solid granule, T3: Pseudomonas fluorescens (Phosphate solubalizing fungi) Solid granule, T4: Bacillus megaterium + Aspergillus niger + Rock Phosphate + Media components (Amino acid, Protein, Starch and Salt) + Plant micro-nutrient mineral media + Pseudomonas fluorescens(Solid granule), T5: Rock Phosphate P2O5 (10.6%), T6: Control

BRIEF DESCRIPTION OF THE FIGURES/DRAWINGS

[0086] FIG. 1: Effect of different ingredients of Bio-phosphate on Maize crop growth leading up to 60 days after sowing.

[0087] FIG. 2: Effect on growth & yield attributes of Maize at Harvesting.