NOVEL GALACTOSIDE INHIBITOR OF GALECTINS

Abstract

An Embodiment of the invention relates to a compound of the general formula. The compound of formula is suitable for use in a method for treating a disorder relating to the binding of a galectin, such as galectin-3 to a ligand in a mammal, such as a human. Furthermore an embodiment of the present invention concerns a method for treatment of a disorder relating to the binding of a galectin, such as galectin-3 to a ligand in a mammal, such as a human.

Claims

1-20. (canceled)

21. A 1,1′-sulfanediyl-di-β-D-galactopyranoside compound of formula (1) ##STR00067## wherein A is selected from a group of formula 2 ##STR00068##  wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, NH.sub.2, NHC(—O)CH.sub.3, methyl optionally substituted with a F, oxo, and OCH.sub.3 optionally substituted with a F; B is selected from a group of formula 3, 4 and 5 ##STR00069##  wherein Het2 is selected from a give or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, NH.sub.2, NHC(═O)CH.sub.3, methyl optionally substituted with a F, Oxo, and OCH.sub.3 optionally substituted with a F, R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F, R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl; or a pharmaceutically acceptable salt or solvate thereof.

22. The compound of claim 21, wherein the compound 3,3′-Dideoxy-3,3′-bis-[4-(2-thiophenyl]-1,1′-sulfanediyl-di-β-D-galactopyranoside is disclaimed.

23. The compound of claim 21, wherein A is selected from formula 2 and B is selected from formula 3.

24. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a give membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom and a six membered hetero aromatic ring containing 1-4 nitrogen atoms; and B is selected from formula 3 and Het2 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, oxo, and OCH.sub.3 optionally substituted with a F.

25. The compound of claims 21, wherein A is selected from formula 2 and Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, oxo, and OCH.sub.3 optionally substituted with a F; and B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atoms and a six membered hetero aromatic ring containing 1-4 nitrogen atoms.

26. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a give membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom and a six membered hetero aromatic ring containing 1-4 nitrogen atoms; and B is selected from formula 3 and Het2 is selected from a give membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom and a six membered hetero aromatic ring containing 1-4 nitrogen atoms.

27. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms substituted with a group selected from Br, F, Cl, CF.sub.3, oxo and OCH.sub.3 and B is selected from formula 3 and Het2 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, oxo, and OCH.sub.3 optionally substituted with a F.

28. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, oxo, NH.sub.2, NHC(═O)CH.sub.3, methyl optionally substituted with a F, and OCH.sub.3 optionally substituted with a F; and B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms substituted with a group selected from Br, F, Cl, oxo, CF.sub.3, and OCH.sub.3.

29. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from Br, F, Cl, oxo, CF.sub.3, NH.sub.2, NHC(═O)CH.sub.3, and OCH.sub.3 and B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from Br, F, Cl, oxo, CF.sub.3, and OCH.sub.3.

30. The compound of claim 21, wherein A is selected from formula 2 and B is selected from formula 4.

31. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally substituted with a group selected from Br, F, Cl, oxo, CF.sub.3, NH.sub.2, NHC(═O)CH.sub.3, and OCH.sub.3; and B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F, such as B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H and F.

32. The compound of claim 21, wherein A is selected from formula 2 and B is selected from formula 5.

33. The compound of claim 21, wherein A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3 and a six membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally substituted with a group selected from Br, F, Cl, oxo, CF.sub.3, and OCH.sub.3; and B is selected from formula 5 and R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl.

34. The compound of claim 21, selected from: 3,3′-Dideoxy-3,3′-bis-[4-(5-fluoropyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3,3′-bis-[4-(6-methoxypyridin-3-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3,3′-bis-[4-(1-methyl-1H-imidazol-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3,3′bis-[4-(3-chloro-5-trifluoromethyl-pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3,3′-bis-[4-(pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3,3′-bis-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(5-fluoropyridin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(pyridin-3-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3,3′-Dideoxy-3-[4-(pyridin-4-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3-[4-((2-acetamid-N-yl)-pyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, 3-[4-(2-aminopyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, and 3,3′-Dideoxy-3-[4-(5-fluoro-1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside.

35. A pharmaceutical composition comprising the compound of claim 21 and optionally a pharmaceutically acceptable additive, such as carrier or excipient.

36. A method for treatment of a disorder relating to the binding of a galectin, such as galectin-3, to a ligand in a mammal, such as a human, wherein a therapeutically effective amount of at least one compound according to claim 21 is administered to a mammal in need of said treatment.

37. The method of claim 36, wherein said disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other intestinal lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0065] In a broad aspect the present invention relates to a 1,1′-sulfanediyl-d-β-D-galactopyranoside compound of formula (1)

##STR00022##

wherein

[0066] A is selected from a group of formula 2

##STR00023##

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, NH.sub.2, NHC(═O)CH.sub.3, methyl optionally substituted with a F, oxo, and OCH.sub.3 optionally substituted with a F;

[0067] B is selected from a group of formula 3, 4 and 5

##STR00024##

wherein Het2 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, NH.sub.2, NHC(═O)CH.sub.3, methyl optionally substituted with a F, Oxo, and OCH.sub.3 optionally substituted with a F,

[0068] R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F,

[0069] R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl; or

[0070] a pharmaceutically acceptable salt or solvate thereof.

[0071] In one embodiment the compound 3,3′-Dideoxy-3,3′-bis-[4-(2-thiophenyl]-1,1′-sulfanediyl-di-β-D-galactopyranoside is disclaimed.

[0072] In an embodiment A is selected from formula 2 and B is selected from formula 3.

[0073] In a further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom.

[0074] In a still further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms.

[0075] In a further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl and imidazolyl.

[0076] In a still further embodiment B is selected from formula 3 and Het2 is selected from a five membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0077] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0078] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from NH.sub.2 and NHC(═O)CH.sub.3.

[0079] In a still further embodiment A is selected from formula 2 and Het1 is selected from a five membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0080] In a further embodiment A is selected from formula 2 and Het1 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0081] In a further embodiment A is selected from formula 2 and Het1 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from NH.sub.2 and NHC(═O)CH.sub.3.

[0082] In a still further embodiment B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom.

[0083] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms.

[0084] In a still further embodiment B is selected from formula 3 and Het2 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl and imidazolyl.

[0085] In a further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom.

[0086] In a still further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms.

[0087] In a further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl and imidazolyl.

[0088] In a still further embodiment B is selected from formula 3 and Het2 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl and imidazolyl.

[0089] In a further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3.

[0090] In a still further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from Br, F, Cl, CF.sub.3, Oxo and OCH.sub.3.

[0091] In a further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl.

[0092] In a further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl substituted with a group selected from NH.sub.2 and NHC(═O)CH.sub.3. Such as pyridinyl substituted with a group selected from NH.sub.2 and NHC(═O)CH.sub.3.

[0093] In a still further embodiment B is selected from formula 3 and Het2 is selected from a five membered heteroaromatic ring, optionally substituted with a group selected from F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0094] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), Oxo, and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0095] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from NH.sub.2, and NHC(═O)CH.sub.3.

[0096] In a still further embodiment A is selected from formula 2 and Het1 is selected from a five membered heteroaromatic ring, optionally substituted with a group selected from F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0097] In a further embodiment A is selected from formula 2 and Het1 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from F, Cl, methyl optionally substituted with a F (e.g. CF.sub.3), Oxo, and OCH.sub.3 optionally substituted with a F (e.g. OCF.sub.3).

[0098] In a still further embodiment B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3.

[0099] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from Br, F, Cl, CF.sub.3, Oxo, and OCH.sub.3.

[0100] In a further embodiment B is selected from formula 3 and Het2 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from NH.sub.2 and NHC(═O)CH.sub.3.

[0101] In a still further embodiment B is selected from formula 3 and Het2 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl.

[0102] In a further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3.

[0103] In a still further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, substituted with a group selected from Br, F, Cl, CF.sub.3, Oxo, and OCH.sub.3.

[0104] In a further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl.

[0105] In a still further embodiment B is selected from formula 3 and Het2 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3.

[0106] In a still further embodiment B is selected from formula 3 and Het2 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl.

[0107] In a further embodiment A is selected from formula 2 and B is selected from formula 4.

[0108] In a still further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; and B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F.

[0109] In a further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, Oxo, and OCH.sub.3; and B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F.

[0110] In a still further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoly, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl; and B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH.sub.3 optionally substituted with a F.

[0111] In a further embodiment B is selected from formula 4 and R.sub.1-R.sub.5 are independently selected from H and F, provided that at least one of R.sub.2-R.sub.4 is selected from a F. In a further embodiment R.sub.1 and R.sub.5 are H, and R.sub.2-R.sub.4 are F.

[0112] In a further embodiment A is selected from formula 2 and B is selected from formula 5.

[0113] In a still further embodiment A is selected from formula 2 and Het1 is selected from a five membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally 1 oxygen atom and optionally one sulphur atom, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; and B is selected from formula 5 and R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl.

[0114] In a further embodiment A is selected from formula 2 and Het1 is selected from a six membered hetero aromatic ring containing 1-4 nitrogen atoms, optionally substituted with a group selected from Br, F, Cl, CF.sub.3, Oxo, and OCH.sub.3; and B is selected from formula 5 and R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl.

[0115] In a still further embodiment A is selected from formula 2 and Het1 is selected from a pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoyl, oxadiazoyl, thiophenyl, and imidazolyl, substituted with a group selected from Br, F, Cl, CF.sub.3, and OCH.sub.3; or pyridonyl; and B is selected from formula 5 and R.sub.6 is selected from C.sub.1-6 alkyl, branched C.sub.3-6 alkyl and C.sub.3-7 cycloalkyl.

[0116] In a further embodiment the compound of formula (1) is selected from [0117] 3,3′-Dideoxy-3,3′-bis-[4-(5-fluoropyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0118] 3,3′-Dideoxy-3,3′-bis-[4-(6-methoxypyridin-3-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside,
3,3′-Dideoxy-3,3′-bis-[4-(1-methyl-1H-imidazol-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0119] 3,3′-Dideoxy-3,3′-bis-[4-(3-chloro-5-trifluoromethyl-pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0120] 3,3′-Dideoxy-3,3′-bis-[4-(pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0121] 3,3′-Dideoxy-3,3′-bis-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, and [0122] 3,3′-Dideoxy-3-[4-(5-fluoropyridin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside.

[0123] In a still further embodiment the compound of formula (1) is selected from [0124] 3,3′-Dideoxy-3-[4-(pyridin-3-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0125] 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0126] 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0127] 3,3′-Dideoxy-3-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0128] 3,3′-Dideoxy-3-[4-(pyridin-4-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0129] 3-[4-((2-acetamid-N-yl)-pyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, [0130] 3-[4-(2-aminopyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside, and [0131] 3,3′-Dideoxy-3-[4-(5-fluoro-1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside.

[0132] In a further aspect the present invention relates to a compound of formula 1 of the present invention for use as a medicine.

[0133] In a still further aspect the present invention relates to a pharmaceutical composition comprising a compound of formula 1 of the present invention and optionally a pharmaceutically acceptable additive, such as carrier or excipient.

[0134] In a further aspect the present invention relates to a compound of formula 1 of the present invention for use in a method for treating a disorder relating to the binding of a galectin, such as galectin-1 and galectin-3 to a ligand in a mammal, such as a human.

[0135] In a further embodiment the disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasing cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other interstitial lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

[0136] In a still further aspect the present invention relates to a method for treatment of a disorder relating to the binding of a galectin, such as galectin-1 and galectin-3, to a ligand in a mammal, such as a human, wherein a therapeutically effective amount of at least one compound of formula (1) of the present invention is administered to a mammal in need of said treatment.

[0137] In a further embodiment of the present invention, the disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other intestinal lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

[0138] Another aspect of the present invention concerns combination therapy involving administering a compound of formula (1) and the present invention together with a therapeutically active compound different from the compound of formula (1) (interchangeable with a “a different therapeutically active compound”). In one embodiment the present invention relates to a combination of a compound of formula (1) and a different therapeutically active compound for use in treatment of a disorder relating to the binding of a galectin, such as galectin-1 and galectin-3, to a ligand in a mammal. Such disorders are disclosed below.

[0139] In an embodiment of the present invention, a therapeutically effective amount of at least one compound of formula (1) of the present invention is administered to a mammal in need thereof in combination with a different therapeutically active compound. In a further embodiment, said combination of a compound of formula (1), in combination with a different therapeutically active compound is administered to a mammal suffering from a disorder selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other intestinal lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

[0140] A non-limiting group of cancers given as examples of cancers that may be treated, managed and/or prevented by administration of a compound of formula (1) in combination with a different therapeutically active compound is selected from: colon carcinoma, breast cancer, pancreatic cancer, ovarian cancer, prostate cancer, fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangeosarcoma, lymphangeoendothelia sarcoma, synovioma, mesothelioma, Ewing's sarcoma, leiomyosarcoma, rhabdomyosarcoma, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystandeocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioblastomas, neuronomas, craniopharingiomas, schwannomas, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroama, oligodendroglioma, meningioma, melanoma, neuroblastoma, retinoblastoma, leukemias and lymphomas, acute lymphocytic leukemia and acute myelocytic polycythemia vera, multiple myeloma, Waldenstrom's macroglobulinemia, and heavy chain disease, acute nonlymphocytic leukemias, chronic lymphocytic leukemia, chronic myelogenous leukemia, Hodgkin's Disease, non-Hodgkin's lymphomas, rectum cancer, urinary cancers, uterine cancers, oral cancers, skin cancers, stomach cancer, brain tumors, liver cancer, laryngeal cancer, esophageal cancer, mammary tumors, childhood-null acute lymphoid leukemia (ALL), thymic ALL, B-cell ALL, acute myeloid leukemia, myelomonocytoid leukemia, acute megakaryocytoid leukemia, Burkitt's lymphoma, acute myeloid leukemia, chronic myeloid leukemia, and T cell leukemia, small and large non-small cell lung carcinoma, acute granulocytic leukemia, germ cell tumors, endometrial cancer, gastric cancer, cancer of the head and neck, chronic lymphoid leukemia, hair cell leukemia and thyroid cancer.

[0141] In some aspects of the present invention, the administration of at least one compound of formula (1) of the present invention and at least one additional therapeutic agent demonstrate therapeutic synergy. In some aspects of the methods of the present invention, a measurement of response to treatment observed after administering both at least one compound of formula (1) of the present invention and the additional therapeutic agent is improved over the same measurement of response to treatment observed after administering either the at least one compound of formula (1) of the present invention or the additional therapeutic agent alone.

[0142] A further aspect of the present invention concerns combination therapy involving administering a compound of formula (1) of the present invention together with an anti-fibrotic compound different form the compound of formula (1) to a mammal in need thereof. In a further embodiment, such anti-fibrotic compound may be selected from the following non-limiting group of anti-fibrotic compounds: pirfenidone, nintedanib, simtuzumab (GS-6624, AB0024), BG00011 (STX100), PRM-151, PRM-167, PEG-FGF21, BMS-986020, FG-3019, MN-001, IW001, SAR156597, GSK2126458, and PBI-4050.

[0143] A still further aspect of the present invention concerns combination therapy involving administering a compound of formula (1) in combination with a further conventional cancer treatment such as chemotherapy or radiotherapy, or treatment with immunostimulating substances, gene therapy, treatment with antibodies and treatment using dendritic cells, to a mammal in need thereof.

[0144] In an embodiment the compound of formula (1) is administered together with at least one additional therapeutic agent selected from an antineoplastic chemotherapy agent. In a further embodiment, the antineoplastic chemotherapeutic agent is selected from: all-trans retinoic acid, Actimide, Azacitidine, Azathioprine, Bleomycin, Carboplatin, Capecitabine, Cisplatin, Chlorambucil, Cyclophosphamide, Cytarabine, Daunorubicin, Docetaxel, Doxifluridine, Doxorubicin, Epirubicin, Etoposide, Fludarabine, Fluorouracil, Gemcitabine, Hydroxyurea, Idarubicin, Irinotecan, Lenalidomide, Leucovorin, Mechlorethamine, Melphalan, Mercaptopurine, Methotrexate, Mitoxantrone, Oxaliplatin, Paclitaxel, Pemetrexed, Revlimid, Temozolomide, Teniposide, Thioguanine, Valrubicin, Vinblastine, Vincristine, Vindesine and Vinorelbine. In one embodiment, a chemotherapeutic agent for use in the combination of the present agent may, itself, be a combination of different chemotherapeutic agents. Suitable combinations include FOLFOX and IFL. FOLFOX is a combination which includes 5-fluorouracil (5-FU), leucovorin, and oxaliplatin. IFL treatment includes irinotecan, 5-FU, and leucovorin.

[0145] In a further embodiment of the present invention, the further conventional cancer treatment includes radiation therapy. In some embodiments, radiation therapy includes localized radiation therapy delivered to the tumor. In some embodiments, radiation therapy includes total body irradiation.

[0146] In other embodiments of the present invention the further cancer treatment is selected from the group of immunostimulating substances e.g. cytokines and antibodies. Such as cytokines may be selected from the group consisting of, but not limited to: GM-CSF, type I IFN, interleukin 21, interleukin 2, interleukin 12 and interleukin 15. The antibody is preferably an immunostimulating antibody such as anti-CD40 or anti-CTLA-4 antibodies. The immunostimulatory substance may also be a substance capable of depletion of immune inhibitory cells (e.g. regulatory T-cells) or factors, said substance may for example be E3 ubiquitin ligases. E3 ubiquitin ligases (the HECT, RING and U-box proteins) have emerged as key molecular regulators of immune cell function, and each may be involved in the regulation of immune responses during infection by targeting specific inhibitory molecules for proteolytic destruction. Several HECT and RING E3 proteins have now also been linked to the induction and maintenance of immune self-tolerance: c-Cbl, Cbl-b, GRAIL, Itch and Nedd4 each negatively regulate T cell growth factor production and proliferation.

[0147] In some embodiments of the present invention the compound of formula (1) is administered together with at least one additional therapeutic agent selected from a checkpoint inhibitor. In some embodiments of the invention, the checkpoint inhibitor is acting on one or more of the following, non-limiting group of targets: CEACAM1, galectin-9, TIM3, CD80, CTLA4, PD-1, PD-L1, HVEM, BTLA, CD160, VISTA, B7-H4, B7-2, CD155, CD226, TIGIT, CD96, LAG3, GITF, OX40, CD137, CD40, IDO, and TDO. These are know targets and some of these targets are described in Melero et al., Nature Reviews Cancer (2015).

[0148] In some embodiments of the present invention the compound of formula (1) is administered together with at least one additional therapeutic agent selected from an inhibitor of indoleamine-2,3-dioxygenase (IDO).

[0149] In some embodiments of the present invention the compound of formula (1) is administered together with at least one additional therapeutic agent selected from one or more inhibitors of the CTLA4 pathway. In some embodiments, the inhibitor of the CTLA4 pathway is selected from one or more antibodies against CTLA4.

[0150] In some embodiments of the present invention the compound of formula (1) is administered together with at least one additional therapeutic agent selected from one or more inhibitors of the PD-1/PD-L pathway. In some embodiments, the one or more inhibitors of the PD-1/PD-L pathway are selected from one or more antibodies against PD-1, PD-L1, and/or PD-L2.

[0151] The skilled person will understand that it may be necessary to adjust or change the order of steps in the process a1 to a11, and such change of order is encompassed by the aspects of the process as described above in the reaction schemes and accompanying description of the process steps.

[0152] Furthermore the skilled person will understand that the processes described above and hereinafter the functional groups of intermediate compounds may need to be protected by protecting group.

[0153] Functional groups that it is desirable to protect include hydroxy, amino and carboxylic acid. Suitable protecting groups for hydroxy include optionally substituted and/or unsaturated alkyl groups (e.g. methyl, allyl, benzyl or tert-butyl), trialkyl silyl or diarylalkylsilyl groups (e.g., t-butyldimethylsilyl, t-butyldipheylsilyl or trimethylsilyl), AcO(acetoxy), TBS(t-butyldimethylsilyl), TMS (trimethylsilyl), PMB (p-methoxybensyl), and tetrahydropyranyl. Suitable protecting groups for carboxylic acid include (C1-C6)-alkyl or benzyl esters. Suitable protecting groups for amino include t-butyloxycarbonyl, benzyloxycarbonyl, 2-(trimethylsilyl)-ethoxy-methyl or 2-trimethylsilylethoxycarbonyl (Teoc). Suitable protecting groups for S include S—C(═N)NH.sub.2, TIPS.

[0154] The protection and deprotection of functional groups may take place before or after any reaction in the above mentioned processes.

[0155] Furthermore the skilled person will appreciate that, in order to obtain compounds of the invention in an alternative, and on some occasions, more convenient, manner, the individual process steps mentioned hereinbefore may be performed in different order, and/or the individual reactions may be performed at a different stage in the overall route (i.e. substituents may be added to and/or chemical transformations performed upon, different intermediates to those mentioned hereinbefore in conjunction with a particular reaction). This may negate, or render necessary, the need for protecting groups.

[0156] In a still further embodiment the compound 1 is on free form. In one embodiment the free form is an anhydrate. In another embodiment the free form is a solvate, such as a hydrate.

[0157] In a further embodiment the compound is a crystalline form. The skilled person may carry out tests in order to find polymorphs, and such polymorphs are intended to be encompassed by the term “crystalline form” as used herein.

[0158] When the compounds and pharmaceutical compositions herein disclosed are used for the above treatment, a therapeutically effective amount of at least one compound is administered to a mammal in need of said treatment.

[0159] The term “C.sub.1-x alkyl” as used herein means an alkyl group containing 1-x carbon atoms, e.g. C.sub.1-5 or C.sub.1-6, such as methyl, ethyl, propyl, butyl, pentyl or hexyl.

[0160] The term “branched C.sub.3-6 alkyl” as used herein means a branched alkyl group containing 3-6 carbon atoms, such as isopropyl, isobutyl, tert-butyl, isopentyl, 3-methylbutyl, 2,2-dimethylpropyl, n-hexyl, 2-methylpentyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl.

[0161] The term “C.sub.3-7 cycloalkyl” as used herein means a cyclic alkyl group containing 3-7 carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and 1-methylcyclopropyl.

[0162] The term “C(═O)” as used herein means a carbonyl group.

[0163] The term “Oxo” as used herein means an oxygen atom with double bonds, also indicated as ═O.

[0164] The term “a five or six membered heteroaromatic ring” as used herein means one five membered heteroaromatic ring or one six membered heteroaromatic ring. The five membered heteroaromatic ring contains 5 ring atoms of which one to four are heteroatoms selected from N, O, and S. The six membered heteroaromatic ring contains 6 ring atoms of which one to five are heteroatoms selected from N, O and S. Examples include thiophene, furan, pyran, pyrrole, imidazole, pyrazole, isothiazole, isooxazole, pyridine, pyrazine, pyrimidine and pyridazine. When such heteroaromatic rings are substituents they are termed thiophenyl, furanyl, pyranyl, pyrrolyl, imidazolyl, pyrazolyl, isothiazolyl, isooxazolyl, pyridinyl, pyrazinyl, pyrimidinyl and pyridazinyl. Also included are oxazoyl, thiazoyl, thiadiazoly, oxadiazoyl, and pyridonyl.

[0165] The term “treatment” and “treating” as used herein means the management and care of a patient for the purpose of combating a condition, such as a disease or a disorder. The term is intended to include the full spectrum of treatments for a given condition from which the patient is suffering, such as administration of the active compound to alleviate the symptoms or complications, to delay the progression of the disease, disorder or condition, to alleviate or relief the symptoms and complications, and/or to cure or eliminate the disease, disorder or condition as well as to prevent the condition, wherein prevention is to be understood as the management and care of a patient for the purpose of combating the disease, condition, or disorder and includes the administration of the active compounds to prevent the onset of the symptoms or complications. The treatment may either be performed in an acute or in a chronic way. The patient to be treated is preferably a mammal; in particular a human being, but it may also include animals, such as dogs, cats, cows, sheep and pigs.

[0166] The term “a therapeutically effective amount” of a compound of formula (1) of the present invention as used herein means an amount sufficient to cure, alleviate or partially arrest the clinical manifestations of a given disease and its complications. An amount adequate to accomplish this is defined as “therapeutically effective amount”. Effective amounts for each purpose will depend on the severity of the disease or injury as well as the weight and general state of the subject. It will be understood that determining an appropriate dosage may be achieved using routine experimentation, by constructing a matrix of values and testing different points in the matrix, which is all within the ordinary skills of a trained physician or veterinary.

[0167] In a still further aspect the present invention relates to a pharmaceutical composition comprising the compound of formula (1) and optionally a pharmaceutically acceptable additive, such as a carrier or an excipient.

[0168] As used herein “pharmaceutically acceptable additive” is intended without limitation to include carriers, excipients, diluents, adjuvant, colorings, aroma, preservatives etc. that the skilled person would consider using when formulating a compound of the present invention in order to make a pharmaceutical composition.

[0169] The adjuvants, diluents, excipients and/or carriers that may be used in the composition of the invention must be pharmaceutically acceptable in the sense of being compatible with the compound of formula (1) and the other ingredients of the pharmaceutical composition, and not deleterious to the recipient thereof. It is preferred that the compositions shall not contain any material that may cause an adverse reaction, such as an allergic reaction. The adjuvants, diluents, excipients and carriers that may be used in the pharmaceutical composition of the invention are well known to a person within the art.

[0170] As mentioned above, the compositions and particularly pharmaceutical compositions as herein disclosed may, in addition to the compounds herein disclosed, further comprise at least one pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier. In some embodiments, the pharmaceutical compositions comprise from 1 to 99 weight % of said at least one pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier and from 1 to 99 weight % of a compound as herein disclosed. The combined amount of the active ingredient and of the pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier may not constitute more than 100% by weight of the composition, particularly the pharmaceutical composition.

[0171] In some embodiments, only one compound as herein disclosed is used for the purposes discussed above.

[0172] In some embodiments, two or more of the compound as herein disclosed are used in combination for the purposes discussed above.

[0173] The composition, particularly pharmaceutical composition comprising a compound set forth herein may be adapted for oral, intravenous, topical, intraperitoneal, nasal, buccal, sublingual, or subcutaneous administration, or for administration via the respiratory tract in the form of, for example, an aerosol or an air-suspended fine powder. Therefore, the pharmaceutical composition may be in the form of, for example, tablets, capsules, powders, nanoparticles, crystals, amorphous substances, solutions, transdermal patches or suppositories.

[0174] Further embodiments of the process are described in the experimental section herein, and each individual process as well as each starting material constitutes embodiments that may form part of embodiments.

[0175] The above embodiments should be seen as referring to any one of the aspects (such as ‘method for treatment’, ‘pharmaceutical composition’, ‘compound for use as a medicament’, or ‘compound for use in a method’) described herein as well as any one of the embodiments described herein unless it is specified that an embodiment relates to a certain aspect or aspects of the present invention.

[0176] All references, including publications, patent applications and patents, cited herein are hereby incorporated by reference to the same extent as if each reference was individually and specifically indicated to be incorporated by reference and was set forth in its entirety herein.

[0177] All headings and sub-headings are used herein for convenience only and should not be construed as limiting the invention in any way.

[0178] Any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.

[0179] The terms “a” and “an” and “the” and similar referents as used in the context of describing the invention are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context.

[0180] Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless other-wise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. Unless otherwise stated, all exact values provided herein are representative of corresponding approximate values (e.g., all exact exemplary values provided with respect to a particular factor or measurement can be considered to also provide a corresponding approximate measurement, modified by “about,” where appropriate).

[0181] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.

[0182] The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise indicated. No language in the specification should be construed as indicating any element is essential to the practice of the invention unless as much is explicitly stated.

[0183] The citation and incorporation of patent documents herein is done for convenience only and does not reflect any view of the validity, patentability and/or enforceability of such patent documents.

[0184] The description herein of any aspect or embodiment of the invention using terms such as “comprising”, “having”, “including” or “containing” with reference to an element or elements is intended to provide support for a similar aspect or embodiment of the invention that “consists of”, “consists essentially of”, or “substantially comprises” that particular element or elements, unless otherwise stated or clearly contradicted by context (e.g., a composition described herein as comprising a particular element should be understood as also describing a composition consisting of that element, unless otherwise stated or clearly contradicted by context).

[0185] This invention includes all modifications and equivalents of the subject matter recited in the aspects or claims presented herein to the maximum extent permitted by applicable law.

[0186] The present invention is further illustrated by the following examples that, however, are not to be construed as limiting the scope of protection. The features disclosed in the foregoing description and in the following examples may, both separately and in any combination thereof, be material for realizing the invention in diverse forms thereof.

Experimental Procedures

Evaluation of Kd Values

[0187] The affinity of compounds S4a-f for galectins were determined by a fluorescence anisotropy assay where the compound was used as in inhibitor of the interaction between galectin and a fluorescein tagged saccharide probe as described Sörme, P., Kahl-Knutsson, B. Huflejt, M., Nilsson, U. J., and Leffler H. (2004) Fluorescence polarization as an analytical tool to evaluate galectin-ligand interactions. Anal. Biochem. 334: 36-47, (Sörme et al., 2004) and Monovalent interactions of Galectin-1 By Salomonsson, Emma; Larumbe, Amaia; Tejler, Johan; Tullberg, Erik; Rydberg, Hanna; Sundin, Anders; Khabut, Areej; Frejd, Torbjorn; Lobsanov, Yuri D.; Rini, James M.; et al, From Biochemistry (2010), 49(44), 9518-9532, (Salomonsson et al., 2010). The assay was adapted to be able to measure the high affinity of the present compound for galectin-3 by using the below probe constructed to have high affinity for galectin-3 based on the structure of Ref 1 which made it possible to use a low concentration of galectin-3 (50 nM). 100 nM albumin was included as a carrier to prevent protein loss at such low concentration of galectin.

##STR00025##

Ref 1. 3,3′-bis-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0188] Kd values for compounds s4a-f, S7, S8a-h and reference compound ref 1

TABLE-US-00001 Galectin-3 Galectin 1 Solubility Solubility Compound Kd Kd buffer* water Example Structure (μM) (μM) (mg/ml) (mg/ml) S4a [00026] 0.010 0.021 2.6 4.3 S4b [00027] 0.012 0.12 S4c [00028] 0.131 0.60 S4d [00029] 0.025 0.74 0.2 S4e [00030] 0.044 0.39 0.007 S4f [00031] 0.042 0.21 0.45 S7 [00032] <0.005 0.04 S8a [00033] 0.002 0.038 0.041 S8b [00034] <0.002 0.080 0.36 S8c [00035] <0.002 0.070 4.6 S8d [00036] 0.001 0.038 1.4 S8e [00037] 0.001 0.072 1.3 S8f [00038] 0.005 0.149 0.77 S8g [00039] <0.002 0.034 1.8 S8h [00040] <0.002 0.055 3.3 Ref 1 [00041] 0.002 0.040 0.4 *0.1 M phosphate buffer pH 6.5

Synthesis

Materials and Methods

[0189] Commercial reagents were used without further purification unless otherwise stated.

[0190] Analytical TLC was performed on silica gel 60-F.sub.254 (Merck) with detection by fluorescence and by immersion in a 10% ethanolic solution of sulfuric acid. Followed by charring.

[0191] Nuclear Magnetic Resonance (NMR) spectra were recorded on a Bruker DRX 400 MHz spectrometer, 400 MHz Varian or a 500 MHz Bruker AVANCE III 500 instrument, at 25° C. Chemical shifts are reported in ppm (δ) using the residual solvent as internal standard. Peak multiplicities are expressed as follow: s, singlet; d, doublet; dd, doublet of doublets; t, triplet; dt, double of triplet; q, quartet; m, multiplet; br s, broad singlet.

[0192] LC-MS were acquired on an Agilent 1100 or Agilent 1200 HPLC coupled with an Agilent MSD mass spectrometer operating in ES (+) ionization mode. Columns: Waters symmetry 2.1×30 mm C18, Chromolith RP-18 2×50 mm or XBridge C18 (4.6×50 mm, 3.5 μm) or Sun-Fire C18 (4.6×50 mm, 3.5 μm). Solvent A water+0.1% TFA and solvent B Acetonitrile+0.1% TFA. Wavelength: 254 nM. ESI-MS was recorded on a Micromass Q-TOF mass spectrometer.

[0193] Preparative HPLC was performed on a Gilson system. A) Flow: 10 ml/min Column: kromasil 100-5-C18 column. Wavelength: 254 nM. Solvent A water+0.1% TFA and solvent B Acetonitrile+0.1% TFA. B) on a Gilson 215, Flow: 25 ml/min Column: XBrige prep C18 10 μm OBD (19×250 mm) column. Wavelength: 254 nM. Solvent A water (10 mM Ammonium hydrogen carbonate) and solvent B Acetonitrile.

[0194] Flash chromatography was performed on a Biotage Sp1 automated system, using Biotage Snap KP-Sil 25 g or 50 g cartridges or by column chromatography on silica gel (Amicon Matrex 35-70 μM, 60 Å).

[0195] General synthesis scheme for compounds S4a-f wherein Y.sub.1 is defined as Het1 and Y2 is defined as He2 and Het.sub.1=Het.sub.2;

##STR00042##

[0196] Copper-catalyzed multi-component reaction of acetylated 3-azido galactoside S1, S2a-f (a) CuI, Et.sub.3N, dry DMF, rt, N.sub.2 atm; (b) 0.5 M NaOMe in MeOH, rt

Synthesis of Example s4a-f

TABLE-US-00002 Yield IUPAC name Alkyne Example (%) 3,3′-Dideoxy-3,3′- bis-[4-(5- fluoropyridin-2-yl)- 1H-1,2,3-triazol-1- yl]-1,1′- sulfanediyl-di-β- D- galactopyranoside [00043] [00044] 85 3,3′-Dideoxy-3,3′- bis-[4-(6- methoxypyridin-3- yl)-1H-1,2,3- triazol-1-yl]-1,1′- sulfanediyl-di-β- D- galactopyranoside [00045] [00046] 87 3,3′-Dideoxy-3,3′- bis-[4-(1-methyl- 1H-imidazol-5-yl)- 1H-1,2,3-triazol-1- yl]-1,1′- sulfanediyl-di-β- D- galactopyranoside [00047] [00048] 94 3,3′-Dideoxy-3,3′- bis-[4-(3-chloro-5- trifluoromethyl- pyridin-2-yl)-1H- 1,2,3-triazol-1-yl]- 1,1′-sulfanediyl-di- β-D- galactopyranoside [00049] [00050] 65 3,3′-Dideoxy-3,3′- bis-[4-(pyrimidin- 5-yl)-1H-1,2,3- triazol-1-yl]-1,1′- sulfanediyl-di-β- D- galactopyranoside [00051] [00052] 86 3,3′-Dideoxy-3,3′- bis-[4-(pyridin-2- yl)-1H-1,2,3- triazol-1-yl]-1,1′- sulfanediyl-di-β- D- galactopyranoside [00053] [00054] 85

S4a) 3,3′-Dideoxy-3,3′-bis-[4-(5-fluoro-2-pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′- sulfanediyl-di-β-D-galactopyranoside

[0197] a) A solution of carbohydrate azide S1 (156 mg, 0.226 mmol), Alkyne (S2a) (41 mg, 0.339 mmol), CuI (2 mg, 0.0113 mmol) in dry DMF (10 mL) in a 25 mL round bottomed flask was stirred under nitrogen for 1 hour. Et.sub.3N (0.016 mL, 0.113 mmol) was then added slowly via syringe. The resulting solution was allowed to stir at room temperature for 12 hours when TLC showed complete conversion of the starting carbohydrate azide (1:2, n-heptane-EtOAc). Solvents were evaporated in vacuo and the residue was dissolved in CH.sub.2Cl.sub.2 (10 mL) and washed successively with aqueous NH.sub.4Cl (2×10 mL) and brine (10 mL). The organic layer was separated, dried (Na.sub.2SO.sub.4) and evaporated in vacuo. The residue was purified by flash chromatography using n-hepatne-EtOAc as eluent to afford pure acetylated glycosylated triazole (3a) in 86% yield.

[0198] b) This was then deprotected using 0.5M NaOMe in MeOH and after the reaction was complete, it was neutralized using DOWEX H.sup.+ resin. The resin was filtered and the crude was purified by flash chromatography using CH.sub.2Cl.sub.2:MeOH as eluent. This afforded the white solid compound S4a in 85% yield. White amorphous solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ: 8.63 (s, 2H, triazole-H), 8.46 (d, 2H, 2.8 Hz, Ar—H), 8.10 (dd, 2H, 8.8 and 4.4 Hz, Ar—H), 7.71 (m, 2H, Ar—H), 4.95-4.92 (m, 4H, H1,H3), 4.70 (dd, 2H, 10.0 Hz, H2), 4.17 (d, 2H, 2.4 Hz, H4), 3.9-3.7 (6H, H5, H6). .sup.13C NMR (CD.sub.3OD, 100 MHz) δ: 161.73, 159.20, 147.92, 147.88, 138.76 138.51, 125.49, 125.30, 123.76, 122.58, 86.50 (C-1), 81.40 (C-5), 69.72, 68.91, 68.45, 62.77 (C-6). HRMS m/z calcd for C.sub.26H.sub.29N.sub.8O.sub.8SF.sub.2 (M+H.sup.+), 651.1797; found, 651.1810.

[0199] Examples s4b-f were made using a similar procedure as compound s4a giving the yields described in the table above, and analytical data as described below;

S4b) 3,3′-Dideoxy-3,3′-bis-[4-(6-methoxypyridin-3-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0200] White amorphous solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ: 8.54 (s, 4H, triazole-H, ArH), 8.05 (d, 2H, 8.4 Hz, ArH), 6.84 (d, 2H, 8.4 Hz, ArH), 4.88 (6H obscured under H.sub.2O, H-1, H-3, H-2), 4.19 (d, 2H, H-4), 3.91 (s, 6H, OCH.sub.3), 3.89-3.71 (m, 6H, H-5, H-6). .sup.13C NMR (CD.sub.3OD, 100 MHz) δ: 165.41, 145.29, 144.82, 121.77, 121.59, 111.99, 86.85 (C-1), 81.42 (C-5), 69.77 (C-4), 68.77, 68.28, 62.89 (C-6), 54.22 (OCH.sub.3). HRMS m/z calcd for C.sub.28H.sub.35N.sub.8O.sub.10S (M+H.sup.+), 675.2197; found, 675.2202.

S4c) 3,3′-Dideoxy-3,3′-bis-[4-(1-methyl-1H-imidazol-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0201] White amorphous solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ: 8.40 (s, 2H, triazole-H), 7.71 (s, 2H, imidazole-H), 7.23 (s, 2H, imidazole-H), 4.92 (6H, obscured under H.sub.2O, H-1, H-2, H-3), 4.14 (d, 2H, 2.4 Hz, H-4), 3.88 (m, 12H, NCH.sub.3, H-5, H-6). .sup.13C NMR (DMSO, 100 MHz) δ: 140.77, 138.24, 128.24, 123.21, 86.83 (C-1), 81.43 (C-5), 69.73, 68.79, 68.28, 62.85, 33.62. HRMS m/z calcd for C.sub.24H.sub.33N.sub.10O.sub.8S (M+H.sup.+), 621.2204; found, 621.2205.

S4d) 3,3′-Dideoxy-3,3′-bis-[4-(3-chloro-5-trifluoromethyl-pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0202] White amorphous solid. .sup.1H NMR (DMSO, 400 MHz) β: 9.02 (d, 2H, 1.2 Hz, ArH), 8.71 (s, 2H, triazole-H), 8.57 (d, 2H, 1.6 Hz, ArH), 5.54 (d, 2H, 6.0 Hz, OH), 5.36 (d, 2H, 7.2 Hz, OH), 4.99 (m, 4H, H-3, H-1), 4.75 (t, 2H, OH), 4.27 (t, 2H, H.sub.2,3 10.0 Hz, H-2), 4.01 (d, 2H, J.sub.3,4 2.8 Hz, H-4), 3.77 (m, 2H, H-5), 3.57 (m, 4H, H-6). .sup.13C NMR (DMSO, 100 MHz) δ: 150.90, 144.94, 143.76, 136.93, 128.69, 126.76, 125.22, 124.65, 121.94, 83.96 (C-1), 79.77 (C-5), 68.05 (C-4), 67.54 (C-3), 67.27 (C-2), 60.65 (C-6). HRMS m/z calcd fro C.sub.28H.sub.26N.sub.8O.sub.8SCl.sub.2F.sub.6Na (M+Na.sup.+), 841.0073; found, 841.0078.

s4e) 3,3′-Dideoxy-3,3′-bis-[4-(pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0203] White amorphous solid. .sup.1H NMR (DMSO, 400 MHz) δ: 9.28 (s, 4H, triazole-H, ArH), 9.16 (s, 2H, ArH), 8.88 (s, 2H, ArH), 5.44 (m, 4H, OH), 4.96 (m, 4H, H-3, H-1), 4.73 (t, 2H, OH), 4.27 (t, 2H, J.sub.2,3 10.0 Hz, H-2), 3.99 (d, 2H, J.sub.3,4 2.8 Hz, H-4), 3.76 (m, 2H, H-5), 3.57 (m, 4H, H-6). .sup.13C NMR (DMSO, 100 MHz) δ: 160.34, 157.46, 153.10, 139.90, 125.33, 125.52, 83.56 (C-1), 79.23 (C-5), 67.43, 67.23, 66.98, 60.21 (C-6). HRMS m/z calcd for C.sub.24H.sub.28N.sub.10O.sub.8SNa (M+Na.sup.+), 639.1710; found, 639.1715.

s4f) 3,3′-Dideoxy-3,3′-bis-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0204] White amorphous solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ: 8.66 (s, 2H, triazole-H), 8.53 (d, 2H, 4.4 Hz, ArH), 8.06 (d, 2H, 7.6 Hz, ArH), 7.90 (t, 2H, 8.0 Hz, 7.6 Hz, ArH), 7.35 (t, 2H, 6.0 Hz, 6.4 Hz, ArH), 4.97 (m, 4H, H-3, H-1), 4.73 (t, 2H, J.sub.2,3 10.4 Hz, H-2), 4.19 (d, 2H, 2.4 Hz, H-4), 3.92 (m, 4H, H-5, H-6), 3.74 (m, 2H, H-6). .sup.13C NMR (CD.sub.3OD, 100 MHz) δ: 149.77, 148.99, 146.66, 137.59, 123.04, 122.46, 120.24, 85.09 (C-1), 80.01 (C-5), 68.33, 67.57, 65.90, 61.37 (C-6). HRMS m/z calcd for C.sub.26H.sub.31N.sub.8O.sub.8S (M+H.sup.+), 615.1986; found, 615.1988.

Synthesis Scheme for Compound S7;

[0205] ##STR00055##

[0206] (a) CuI, Et.sub.3N, dry DMF, rt, N.sub.2 atm; (b) 0.5M NaOMe in MeOH, rt

Synthesis of Example S7

[0207]

TABLE-US-00003 Yield IUPAC name Alkyne step a Alkyne 2 Example (%) 3,3′-Dideoxy-3-[4- (5-fluoro-2- pyridin-2-yl)-1H- 1,2,3-triazol-1-yl]- 3′-[4-(3,4,5- trifluorophenyl)- 1H-1,2,3-triazol-1- yl]1,1′-sulfanediyl- di-β-D- galactopyranoside [00056] [00057] [00058] 84

[0208] a) A solution of carbohydrate azide (S1) (300 mg, 0.454 mmol), Alkyne (S2g) (70 mg, 0.454 mmol), CuI (4.3 mg, 0.022 mmol) in dry DMF (10 mL) in a 25 mL round bottomed flask was stirred under nitrogen for 1 hour. Et.sub.3N (0.031 mL, 0.227 mmol) was then added slowly via syringe. The resulting solution was allowed to stir at room temperature for 12 hours when TLC showed complete conversion of the starting carbohydrate azide (1:2, n-heptane-EtOAc). Solvents were evaporated in vacuo and the residue was dissolved in CH.sub.2Cl.sub.2 (10 mL) and washed successively with aqueous NH.sub.4Cl (2×10 mL) and brine (10 mL). The organic layer was separated, dried (Na.sub.2SO.sub.4) and evaporated in vacuo. The residue was purified by flash chromatography using n-heptane-EtOAc as eluent to afford pure acetylated glycosylated triazole (S5) in 41% yield.

[0209] b) S5 was then deprotected using 0.5M NaOMe in MeOH and after the reaction was complete, it was neutralized using DOWEX H.sup.+ resin. The resin was filtered and the crude was purified by flash chromatography using CH.sub.2Cl.sub.2:MeOH as eluent. This afforded the white amorphous compound S6 in 85% yield.

[0210] c) Compound S6 (76 mg, 0.093 mmol), Alkyne (S2a) (16.9 mg, 0.139 mmol), CuI (0.88 mg, 0.0046 mmol) in dry DMF (5 mL) was stirred under nitrogen for 1 hour. Et.sub.3N (6.0 μL, 0.046 mmol) was then added slowly via syringe. The resulting solution was allowed to stir at room temperature for 12 hours when TLC showed complete conversion of the starting carbohydrate azide (6:1 CH.sub.2Cl.sub.2:MeOH). Solvents were evaporated in vacuo and the residue was purified by flash chromatography using CH.sub.2Cl.sub.2:MeOH as eluent to afford pure glycosylated triazole (1d) in 84% yield.

[0211] White amorphous solid. .sup.1H NMR (CD.sub.3OD, 400 MHz) δ: 8.63, 8.59 (2s, 2H, triazole-H), 8.47 (bs, 1H, Ar—H), 8.09 (bs, 1H, Ar—H), 7.72 (m, 1H, Ar—H). 7.63 (m, 1H, Ar—H) 4.95-4.91 (m, 3H, H1, H1′, H3), 4.75-4.63 (m, 3H, H2, H2′, H3′), 4.17 (dd, 2H, H4, H4′), 3.91-3.80 (m, 4H, H5, H5′, H6, H6′), 3.73-3.69 (m, 2H, H6, H6′). .sup.13C NMR (CD.sub.3OD, 100 MHz) δ: 154.14, 151.67, 151.57, 147.92, 147.66, 145.52, 141.73, 139.24, 138.77, 138.52, 128.09, 125.51, 125.31, 123.74, 122.79, 122.57, 110.82, 110.75, 110.65, 110.59, 86.61 (C-1), 86.52 (C-1′), 81.42 (C-5), 81.35 (C-5′), 69.77, 69.64, 68.92, 68.89, 68.44, 68.37, 62.81, 62.77. HRMS m/z calcd for C.sub.27H.sub.27N.sub.7O.sub.8SF.sub.4.

Synthesis of Examples S8a-h

S8a) 3,3′-3-[4-(pyridin-3-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0212] ##STR00059##

[0213] t (69 mg) and 3-ethynylpyridine (30 mg) was dissolved in MeCN (5 mL, dry) and stirred at r.t. Copper(I) iodide (8 mg) was added followed by Hünig's base (55 μL) and the mixture was heated to 50° C. After 18 h the mixture was filtered and concentrated. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded the title compound as a white solid (65 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 9.21 (bs, 1H), 8.79 (s, 1H), 8.69 (bs, 1H), 8.68 (d, J=8.2 Hz, 1H), 8.57 (s, 1H), 7.88 (bs, 1H), 7.65-7.56 (m, 2H), 4.99-4.90 (m, 4H), 4.75-4.65 (m, 2H), 4.22-4.14 (m, 2H), 3.92-3.81 (m, 4H), 3.75-3.72 (m, 2H). ESI-MS m/z calcd for [C.sub.27H.sub.29F.sub.3N.sub.7O.sub.8S].sup.+ (M+H).sup.+: 668.17; found: 668.25.

S8b) 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-5-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0214] ##STR00060##

[0215] Intermediate 1 (92 mg) and 5-[2-(trimethylsilyl)ethynyl]-pyridine (65 mg) was dissolved in MeCN (5 mL, dry) and stirred at r.t. Copper(I) iodide (24 mg) was added followed by Hünig's base (65 μL) and the mixture was heated to 50° C. After 18 h cesium fluoride (5 mg) was added and the temperature increased to 70° C. After 24 h the mixture was filtered and concentrated. The residue was purified by HPLC (Xterra/MeCN:H.sub.2O:25 mM NH.sub.3). Freeze drying afforded the title compound as a white solid (73 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 9.25 (s, 2H), 9.13 (s, 1H), 8.76 (s, 1H), 8.58 (s, 1H), 7.65-7.56 (m, 2H), 5.01-4.88 (m, 4H), 4.75 (q, J=10.3 Hz, 2H), 4.17 (dd, J=8.7, 2.4 Hz, 2H), 3.95-3.78 (m, 4H), 3.73 (dd, J=11.3, 3.5 Hz, 2H). ESI-MS m/z calcd for [C.sub.26H.sub.28F.sub.3N.sub.8O.sub.8S].sup.+ (M+H).sup.+: 669.16; found: 669.15.

S8c) 3,3′-Dideoxy-3-[4-(1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0216] ##STR00061##

[0217] Intermediate 1 (71 mg) and 2-[2-(trimethylsilyl)ethynyl]-pyridine (48 mg) was dissolved in MeCN (5 mL, dry) and stirred at r.t. Copper(I) iodide (19 mg) was added followed by Hünig's base (50 μL) and the mixture was heated to 50° C. After 18 h cesium fluoride (5 mg) was added and the temperature increased to 70° C. After 24 h the mixture was filtered and concentrated. The residue was purified by HPLC (Xterra/MeCN:H.sub.2O:25 mM NH.sub.3). Freeze drying afforded the title compound as a white sold (47 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 8.83 (bs, 2H), 8.59 (s, 1H), 7.65-7.56 (m, 2H), 7.42 (bs, 1H), 4.98-4.87 (m, 4H), 4.77-4.69 (m, 2H), 4.23-4.12 (m, 2H), 3.92-3.80 (m, 4H), 3.75-3.70 (m, 2H). ESI-MS m/z calcd for [C.sub.26H.sub.28F.sub.3N.sub.8O.sub.8S].sup.+ (M+H).sup.+: 669.16; found: 669.15.

S8d) 3,3′-Dideoxy-3-[4-(pyridin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0218] ##STR00062##

[0219] Intermediate 1 (50 mg), 2-ethynylpyridine (50 mg) and copper(I) iodide (9 mg) were mixed and degassed (argon) in acetonitrile (6 mL). Hünig's base (100 μl) was added and the mixture was stirred at r.t. over night. It was then concentrated and purified by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.5:95). The appropriate fractions were concentrated and the residue dissolved in methanol (10 mL). NaOMe (1M in MeOH, 1.0 mL) was added and the mixture stirred 2 h. TFA (0.2 mL) was added and the mixture concentrated in vacuo. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded the title compound as a white solid (13 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 8.65 (s, 1H), 8.60 (s, 1H), 8.56 (s, 1H), 8.09 (s, 1H), 7.93 (d, J=8.0 Hz, 1H), 7.66-7.54 (m, 2H), 7.37 (s, 1H), 4.97-4.87 (m, 4H), 4.74 (q, J=10.7 Hz, 2H), 4.16 (d, J=9.9 Hz, 2H), 3.94-3.78 (m, 4H), 3.71 (dt, J=10.5, 4.8 Hz, 2H). ESI-MS m/z calcd for [C.sub.27H.sub.29F.sub.3N.sub.7O.sub.8S].sup.+ (M+H).sup.+: 668.17; found: 668.15.

S8c) 3,3′-Dideoxy-3-[4-(pyridin-4-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0220] ##STR00063##

[0221] 4-Ethynylpyridine hydrochloride (104 mg) was suspended in MeCN (10 mL) while bubbling argon through. Hünig's base (130 μL) was added and the mixture stirred 10 min. Intermediate 2 (45 mg) and copper(I) iodide (6 mg) were added and the mixture stirred 5 min. More Hünig's base (130 μl) was added, the vial closed and stirred at r.t. over night. The mixture was concentrated in vacuo, dissolved in EtOAc and filtered through a small plug of silica. The filtrate was concentrated and the residue dissolved in methanol (10 mL). NaOMe (1M in MeOH, 0.5 mL) was added and the mixture stirred at r.t. 2 h. TFA (0.2 mL) was added and the mixture concentrated in vacuo. The residue was purified by HPLC (Xterra/MeCN:H.sub.2O:25 mM NH.sub.3). Freeze drying afforded a white solid (5 mg). .sup.1H NMR (400 MHz, Methanol-d4) δ 9.01 (s, 1H), 8.76 (d, J=6.2 Hz, 2H), 8.56 (s, 1H), 8.34 (d, J=6.2 Hz, 2H), 7.65-7.57 (m, 2H), 5.08-4.89 (m, 4H, 4.68 (td, J=10.1, 5.2 Hz, 2H), 4.18 (t, J=3.8 Hz, 2H), 3.86 (ddt, J=24.8, 13.3, 6.4 Hz, 4H), 3.73 (dt, J=11.3, 4.2 Hz, 2H). ESI-MS m/z calcd for [C.sub.27H.sub.29F.sub.3N.sub.7O.sub.8S].sup.+ (M+H).sup.+: 668.17; found: 668.25.

S8f) 3-[4-((2-acetamid-N-yl)-pyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0222] ##STR00064##

[0223] Intermediate 2 (40 mg) was dissolved in MeCN (10 mL, dry) and 5-ethynyl-2-pyridinamine (12 mg) was added and the mixture was stirred at r.t. under argon. Copper(I) iodide (5 mg) was added followed by Hünig's base (10 μL). After 18 h 5-ethynyl-2-pyridinamine (12 mg) was added and after an additional 6 h the mixture was filter and concentrated down. The residue was dissolved in MeOH (10 mL, dry) and NaOMe (1M in MeOH, 0.50 mL) was added and the mixture was stirred at r.t. After 18 h HOAc (1 mL) was added and the mixture concentrated down. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded a white fluffy solid (15 mg). The material was dissolved in pyridine (2.5 mL) and Acetic anhydride (2.0 mL) was added and the mixture was stirred at r.t. After 18 h the mixture was concentrated down and purified by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.0:100). The residue was dissolved in MeOH (5 mL, dry) and NaOMe (1M in MeOH, 0.25 mL) was added and the mixture was stirred at r.t. After 1 h CH.sub.2Cl.sub.2 (5 mL) and NaOMe (1M in MeOH, 0.25 mL) was added and after an additional 30 min HOAc (1 mL) was added and the mixture was concentrated down. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded a white fluffy solid (4 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 8.79-8.73 (m, 1H), 8.62-8.56 (m, 3H), 8.24 (d, J=7.7 Hz, 1H), 8.08 (s, 1H), 7.63-7.56 (m, 2H), 4.93-4.72 (m, 6H), 4.20-4.13 (m, 2H), 3.92-3.78 (m, 4H), 3.72 (dd, J=11.1, 3.9 Hz, 2H), 2.20 (s, 3H). ESI-MS m/z calcd for [C.sub.29H.sub.32F.sub.3N.sub.8O.sub.9S].sup.+ (M+H).sup.+: 725.19; found: 725.20.

S8g) 3-[4-(2-aminopyridin-5-yl)-3,3′-dideoxy-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0224] ##STR00065##

[0225] Intermediate 2 (40 mg) was dissolved in MeCN (7 mL, dry) and 5-ethynyl-2-pyridinamine (21 mg) was added and the mixture was stirred at r.t. under argon. Copper(I) iodide (18 mg) was added followed by Hünig's base (25 μL) and the mixture was heated to 27° C. After 18 h 5-ethynyl-2-pyridinamine (18 mg) was added and the temperature increased to 35° C. After an additional 2 h copper(I) iodide (5 mg) was added followed by Hünig's base (25 μL). After 5 h the temperature was lowered to 27° C., MeCN (7 mL, dry) was added followed by 5-ethynyl-2-pyridinamine (41 mg), copper(I) iodide (32 mg), and Hünig's base (50 μL). The mixture was stirred for 18 h and then concentrated down and purified by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.0:100). The residue was dissolved in MeOH (10 mL, dry) and NaOMe (1M in MeOH, 0.50 mL) was added and the mixture was stirred at r.t. After 2 h HOAc (1 mL) was added and the mixture was concentrated down. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded a white fluffy solid (10 mg). .sup.1H NMR (400 MHz, Methanol-d4) δ 8.55 (d, J=2.5 Hz, 2H), 8.33-8.26 (m, 2H), 7.66-7.57 (m, 2H), 7.08 (d, J=9.9 Hz, 1H), 4.97-4.86 (m, 4H), 4.69-4.57 (m, 2H), 4.19-4.13 (m, 2H), 3.93-3.79 (m, 4H), 3.77-3.67 (m, 2H). ESI-MS m/z calcd for [C.sub.27H.sub.30F.sub.3N.sub.8O.sub.8S].sup.+ (M+H).sup.+: 683.13; found: 683.10.

S8h) 3,3′-Dideoxy-3-[4-(5-fluoro-1,3-pyrimidin-2-yl)-1H-1,2,3-triazol-1-yl]-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0226] ##STR00066##

[0227] 2-Chloro-5-fluoro-pyrimidine (1.0 mL) was dissolved in DME (10 mL). TMS-Br (1.5 mL) was added and the mixture stirred at 150° C. in a closed vial for 1.5 h. The mixture was allowed to cool, then concentrated in vacuo. The residue was dissolved in CH.sub.2Cl.sub.2 and filtered through a plug of silica. The filtrate was concentrated in vacuo to give a pale yellow liquid (1.54 g). This compound, 2-bromo-5-fluoropyrimidine (containing some 2-chloro-5-fluoro-pyrimidine), (0.75 g) was dissolved in triethylamine (7 mL) under an argon atmosphere. TMS-acetylene (0.6 mL) was added followed by bis(triphenylphosphine)palladium(II) dichloride (15 mg) and copper(I) iodide (30 mg). After heating the mixture at 50° C. 5 h, it was concentrated in vacuo. The residue was purified by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.5:95) and 5-fluoro-2-[2-(trimethylsilyl)ethynyl]pyrimidine was isolated as a colorless oil (300 mg, 38% purity).

[0228] 5-Fluoro-2-[2-(trimethylsilyl)ethynyl]pyrimidine (210 mg, 38% purity) was dissolved in MeCN (10 mL) while bubbling nitrogen through. TBAF (120 mg) was added. After 2 min, Intermediate 2 (67 mg) in acetonitrile (5 mL) and copper(I) iodide (8 mg) were added and the mixture stirred 5 min. Hünig's base (400 μL) was added, the vial closed and stirred at (room temperature) r.t. overnight and then at 50° C. for 24 h. The mixture was concentrated in vacuo and the residue filtered through silica (CH.sub.2Cl.sub.2:MeOH 9:1). Evaporation of the solvents in vacuo afforded a brown residue. This was dissolved in MeOH (15 mL) and NaOMe (1M in MeOH, 1 ml) was added and the mixture stirred for 2 h. TFA (0.2 mL) was added and the mixture concentrated in vacuo. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded the product as an off-white power (32 mg). .sup.1H NMR (400 MHz, Methanol-d4) δ 8.72 (d, J=3.1 Hz, 3H), 8.58 (d, J=1.8 Hz, 1H), 7.61 (t, J=7.6 Hz, 2H), 5.00-4.97 (m, 4H), 4.73 (q, J=9.5 Hz, 2H), 4.22-4.12 (m, 2H), 3.92-3.78 (m, 4H), 3.72 (d, J=10.9 Hz, 2H). ESI-MS m/z calcd for [C.sub.26H.sub.27F.sub.4N.sub.8O.sub.8S].sup.+ (M+H).sup.+: 687.15; found: 687.15.

Synthesis of Intermediates to Examples S8a-h

Intermediate 1

3-Azido-3,3′-dideoxy-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0229] 2,2′,4,4′,5,5′,6,6′-Hexa-O-acetyl-3,3′-diazido-3,3′-dideoxy-1,1′-sulfanediyl-di-β-D-galactopyranoside (131 mg) and trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (45 μl) were mixed in acetonitrile (5 mL) and degassed (argon). Cesium fluoride (30 mg) was added and the mixture stirred 5 min. Copper(I) iodide (4 mg) was added, followed by Hünig's base (100 μl). The mixture was stirred at r.t. overnight and then concentrated in vacuo. It was then evaporated on to silica and purified by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.5:95). The appropriate fractions were concentrated and the residue dissolved in methanol (10 mL). 1M sodium methoxide in methanol (1.5 ml) was added and the mixture stirred 2 h. TFA (0.2 ml) was added and the mixture concentrated in vacuo. The residue was purified by HPLC (C.sub.18/MeCN:H.sub.2O:0.1% TFA). Freeze drying afforded a white solid (61 mg).

Intermediate 2

2,2′,4,4′,5,5′,6,6′-Hexa-O-acetyl-3-azido-3,3′-dideoxy-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside

[0230] Intermediate 1 (150 mg) was dissolved in pyridine (5 mL). Acetic anhydride (0.50 mL) was added and mixture stirred overnight and then concentrated in vacuo. The residue was dissolved in CH.sub.2Cl.sub.2 (DCM) and filtered through silica (2 g), eluted with 2% MeOH in DCM. The filtrate was concentrated in vacuo to afford Intermediate 2 as a white solid (209 mg). .sup.1H NMR (400 MHz, Methanol-d.sub.4) δ 8.58 (d, J=1.2 Hz, 2H), 7.60 (dd, J=8.5, 6.5 Hz, 4H), 4.90 (d, J=3.3 Hz, 4H), 4.72 (t, J=10.1 Hz, 2H), 4.16 (d, J=2.8 Hz, 2H), 3.94-3.78 (m, 4H), 3.72 (dd, J=11.3, 4.4 Hz, 2H).

Intermediate 2, Alternative Synthesis

2,4,6-Tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranosyl bromide

[0231] 1,2,4,6-Tetra-O-acetyl-3-azido-deoxy-β-D-galactopyranoside (1.99 g) and titanium tetrabromide (2.7 g) were mixed in EtOAc (100 ml) and stirred at 27° C. 48 h. Washed with aq. 5% NaHCO.sub.3 (100 ml) and brine (100 ml). Purification by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.5:95) afforded 2.01 g of 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranosyl bromide. .sup.1H NMR (400 MHz, Chloroform-d) δ 6.71 (d, J=3.8 Hz, 1H), 5.50 (d, J=2.5 Hz, 1H), 4.95 (dd, J=10.6, 3.8 Hz, 1H), 4.42 (t, J=6.4 Hz, 1H), 4.22-4.03 (m, 3H), 2.18 (s, 3H), 2.17 (s, 3H), 2.08 (s, 3H).

Tri-isopropylsilyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-β-D-galactopyranoside

[0232] 2,4,6-Tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranosyl bromide (370 mg) was dissolved in MeCN (15 mL, dry) and stirred at r.t. under argon for five minutes. K.sub.2CO.sub.3 (390 mg, dry) was added followed by TIPSSH (305 μL). After 200 minutes the mixture was concentrated down, re-dissolved in CH.sub.2Cl.sub.2 and washed twice with water. The water phase was extracted once with CH.sub.2Cl.sub.2 and the combined organic phase was dried (phase separator) and concentrated. Purification by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 100:0.fwdarw.50:50) afforded 312 mg of tri-isopropylsilyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-β-D-galactopyranoside. .sup.1H NMR (400 MHz, Chloroform-d) δ 5.45 (d, J=2.5 Hz, 1H), 5.23 (t, J=9.8 Hz, 1H), 4.63 (d, J=9.5 Hz, 1H), 4.14 (dd, J=11.5, 5.5 Hz, 1H), 4.03 (dd, J=11.5, 7.2 Hz, 1H), 3.82 (t, J=6.3 Hz, 1H), 3.56 (dd, J=10.1, 3.3 Hz, 1H), 2.18 (s, 3H), 2.15 (s, 3H), 2.05 (s, 3H), 1.32-1.23 (m, 3H), 1.18-1.06 (m, 18H).

1,2,4,6-Tetra-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside

[0233] Trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (1120 μL) was dissolved in MeCN (50 mL, dry) and stirred at r.t. under argon and triethylamine trihydrofluoride (435 μL) was added. After 25 minutes 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (1025 mg) was added followed by copper(I) iodide (138 mg) and Hünig's base (2.00 mL). After 18 h brine was added and the mixture was extracted three times with ether. The organic phase was washed once with brine, dried (Na.sub.2SO.sub.4) and concentrated. Re-crystallization from EtOH afforded 1.19 g of 1,2,4,6-tetra-O-acetyl-3-deoxy-3-[-4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside. .sup.1H NMR (400 MHz, Chloroform-d) δ 7.80 (s, 1H), 7.47-7.38 (m, 2H), 5.86 (d, J=9.1 Hz, 2H), 5.58 (s, 1H), 5.19 (d, J=9.2 Hz, 1H), 4.30-4.08 (m, 3H), 2.18 (s, 3H), 2.08 (s, 3H), 2.06 (s, 3H), 1.91 (s, 3H). ESI-MS m/z calcd for [C.sub.22H.sub.23F.sub.3N.sub.3O.sub.9].sup.+ (M+H).sup.+: 530.1; found 530.1.

2,4,6-Tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl bromide

[0234] 1,2,4,6-tetra-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside (273 mg) was suspended in CH2Cl.sub.2/AcOH (1:1, 4 mL) and stirred at r.t. Acetic anhydride (1 mL) was added followed by HBr (33% in AcOH, 2 mL). After 20 h excess HBr was purged away with argon and the mixture was concentrated. Purification by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 95:5.fwdarw.5:95) afforded 263 mg of 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl bromide. .sup.1H NMR (400 MHz, Chloroform-d) δ 7.79 (s, 1H), 7.49-7.40 (m, 2H), 6.89 (d, J=3.8 Hz, 1H), 5.82 (dd, J=11.4, 3.9 Hz, 1H), 5.63 (s, 1H), 5.32 (d, J=11.2 Hz, 1H), 4.65 (t, J=6.5 Hz, 1H), 4.25 (dd, J=11.7, 6.3 Hz, 1H), 4.15 (dd, J=11.9, 6.4 Hz, 1H), 2.07 (s, 6H), 1.97 (s, 3H). ESI-MS m/z calcd for [C.sub.20H.sub.20BrF.sub.3N.sub.3O.sub.7].sup.+ (M+H).sup.+: 550.0; found: 550.0.

2,2′,4,4′,5,5′,6,6′-Hexa-O-acetyl-3-azido-3,3′-dideoxy-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside (Intermediate 2)

[0235] Tri-isopropylsilyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-β-D-galactopyranoside (308 mg) was dissolved in MeCN (10 mL, dry) and stirred at r.t. under argon. 2,4,6-Tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl bromide (405 mg) dissolved in MeCN (10 mL, dry) was added. After five minutes TBAF (235 mg) dissolved in MeCN (5 mL, dry) was added. After two minutes the mixture was cooled to 0° C. and after another three minutes the mixture was concentrated. Purification by flash chromatography (SiO.sub.2/Petroleum ether:EtOAc 100:0.fwdarw.0:100) afforded 262 mg of 2,2′,4,4′,5,5′,6,6′-hexa-O-acetyl-3-azido-3,3′-dideoxy-3′-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1,1′-sulfanediyl-di-β-D-galactopyranoside. .sup.1H NMR (400 MHz, Chloroform-d) δ 7.83 (s, 1H), 7.47-7.41 (m, 2H), 5.74 (t, J=10.4 Hz, 1H), 5.62 (d, J=2.4 Hz, 1H), 5.51 (d, J=2.3 Hz, 1H), 5.24 (t, J=10.0 Hz, 2H), 5.17 (dd, J=10.9, 3.1 Hz, 1H), 4.97 (d, J=9.8 Hz, 1H), 4.83 (d, J=9.9 Hz, 1H), 4.21 (dt, J=21.3, 5.6 Hz, 6H), 4.15-4.07 (m, 2H), 3.92 (t, J=6.4 Hz, 1H), 3.68 (dd, J=10.0, 3.3 Hz, 1H), 2.20 (s, 3H), 2.17 (s, 3H), 2.12 (s, 3H), 2.09 (s, 3H), 2.07 (s, 3H), 1.93 (s, 3H). ESI-MS m/z calcd for [C.sub.32H.sub.36F.sub.3N.sub.6O.sub.14S].sup.+ (M+H).sup.+: 817.2; found: 817.2

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