A BIODEGRADABLE NANO - ENCAPSULATED DISINFECTANT COMPOSITION

Abstract

A disinfectant composition contains myrrh tree plant extract, highbush cranberry leaf extract, sumac leaf extract, juniper fruit extract, jasmine and/or daisy flower extract, apple and sunflower face pomace, silver nanoparticle and pure water. In particular, disclosed is a biodegradable nano-encapsulated vegan and ecological disinfectant composition containing herbal synthesized silver nanoparticles which is effective in providing personal hygiene with general and public areas, especially the health sector being in the first place, and a production method thereof.

Claims

1. A nano-encapsulated disinfectant composition comprising herbal synthesized silver nanoparticles, the composition further comprising myrrh tree plant extract, highbush cranberry leaf extract, sumac leaf extract, juniper fruit extract, jasmine and/or daisy flower extract, apple and sunflower face pomace, silver nanoparticle and pure water.

2. The composition according to claim 1, comprising 1-2.5% by weight myrrh tree plant extract, 0.7-1.3% by weight highbush cranberry leaf extract, 0.6-2.2% by weight sumac leaf extract, 0.3-0.9% by weight jasmine and/or daisy flower extract, 2-4.5% by weight juniper fruit extract, 0.74-1.2% by weight apple pomace, 0.9-1.4% by weight sunflower face pomace, 0.037-0.059% by weight silver nanoparticle and 86-93% by weight pure water.

3. The composition according to claim 1, comprising 2.067% by weight myrrh tree plant extract, 1.1% by weight highbush cranberry leaf extract, 1.5% by weight sumac leaf extract, 1% by weight jasmine or daisy flower extract, 3.4% by weight juniper fruit extract, 0.98% by weight apple pomace, 1.25% by weight sunflower face pomace, 0.044% by weight silver nanoparticle and 88.659% by weight pure water.

4. A production method of a nano-encapsulated disinfectant composition comprising herbal synthesized silver nanoparticles wherein the method comprises the following process steps: i. Brewing myrrh tree, highbush cranberry leaf and sumac leaf in water, ii. Extraction of juniper fruits in water, iii. Extraction of jasmine and/or daisy plants in water/vegetative alcohol, iv. Gelling apple and sunflower face pomace in water, obtaining pectin with lactic and citric acid, v. Preparation of solution with pure silver, acetic acid and hydrogen peroxide, vi. Mixing the prepared solution with the vegetative extracts obtained in process steps (i), (ii), (iii), and vii. Coating the nanoparticle obtained in step (vi) with the nano-capsule material obtained in step (iv).

5. The method according to claim 4, wherein process step (i) comprises the processes of brewing the Myrrh tree plant in pure water first at 65-75° C. for 20-25 minutes by occasionally mixing; brewing at 65-85° C. for 10-15 minutes by occasionally mixing by adding equal amounts of powdered Highbush cranberry leaf and Sumac plants over it; subjecting to ultrasonification process in an ultrasonic device at 40° C. for 10-15 minutes for increasing the speed of extraction; shock cooling of the obtained extract and subjecting to ultrasonification process for 10-15 minutes; subjecting to shock cooling process and centrifuging at +1° C. at 4500-5500 rpm for 10-15 minutes and storing at +4° C.

6. The method according to claim 4, wherein process step (ii) comprises the processes of heating Juniper fruits in pure water first at 85-95° C. for 20-30 minutes; subjecting to ultrasonification process for 10-15 minutes, then leaving it to fermentation in the closed container at 45-55° C. for 5-10 days; shock cooling of the obtained extract; and centrifuging at +1° C. at 4500-5500 rpm for 10-15 minutes and storing at +4° C.

7. The method according to claim 4 wherein process step (iii) comprises the processes of brewing jasmine (and/or daisy) flowers first at 35-45° C. for 5-10 minutes by adding 54% vegetative alcohol; then at 50-60° C. for 20-30 minutes by adding pure water by occasionally mixing; cooling in ice bath by subjecting to shock cooling and subjecting the cooled extract to ultrasonification process for 10-15 minutes in ultrasonic device; centrifuging at +1° C. at 4500-5500 rpm for 5-7 minutes; separation of solid and liquid extract parts by filtering through filter paper having 11 μm pore diameter.

8. The method according to claim 4 wherein process step (v) comprises the processes of weighing pure silver in the ratio of 0.037-0.059% and treating with acetic acid and hydrogen peroxide in the ratio of 0.5:2; adding the extracts obtained in process steps (i) (ii) to the solution in bottle number 5 and mixing at 750-850 rpm for 5-10 minutes, and addition of the extract obtained in process step (iii) and mixing at 750-850 rpm for 20-30 minutes.

9. The method according to claim 4 wherein process step (iv) comprises the processes of putting 200-350 grams of apple pomace and adding 20-30 ml of pure water over it; smashing for 1-3 minutes, mixing at 250-300 rpm to bring to boiling point at 80-90° C. for 25-30 minutes; adding citric and lactic acid over it and mixing and cooling; centrifuging at 5300 rpm for 10-15 minutes and filtering through filter paper and leaving at 60-80° C. for 20-25 minutes.

10. The method according to claim 4 wherein process step (iv) comprises the processes of putting 150-250 grams of sunflower face pomace and adding 400-500 ml of pure water over it; heating at 90-95° C. for 35-40 minutes; addition of citric and lactic acid and mixing continuously at 250-350 rpm and smashing for 1-3 minutes; cooling for 20-30 minutes and centrifuging at 5300 rpm for 10-15 minutes; filtering through filter paper and heating at 30-40° C. for 20-25 minutes.

11. The method according to claim 4 wherein process step (vii) comprises the processes of taking the supernatant obtained in process step (iv) from apple pomace and adding dropwise slowly to the mixture obtained in process step (v) which is mixed continuously at 40° C.

12. The method according to claim 4 wherein process step (vii) comprises the processes of taking the supernatant obtained in process step (iv) from sunflower face pomace and adding dropwise slowly to the mixture obtained in process step (v) which is mixed continuously at 40° C.

Description

BRIEF DESCRIPTION OF FIGURES

[0048] FIG. 1 is a graph showing the nanosilver size distribution in the composition of the invention.

[0049] FIG. 2 is a graph showing the Zeta potential in the composition of the invention.

DETAILED DESCRIPTION OF THE INVENTION

[0050] In this detailed description, disinfectant composition of the invention and production method thereof are described aimed at only better understanding of the subject and without forming any limiting effects.

[0051] The invention relates to a biodegradable nanocapsule disinfectant composition comprising herbal synthesized silver nanoparticle and the production method of this composition.

[0052] The disinfectant composition of the invention in its most fundamental form comprises myrrh tree extract, highbush cranberry leaf extract, sumac plant extract, juniper fruit extract, jasmine and/or daisy flower extract, apple and sunflower face pomace, silver nanoparticle and pure water.

TABLE-US-00001 TABLE 1 The content of the composition of the invention Preferred Usable amount by amount by Content weight (%) weight (%) Myrrh (Commiphora myrrha) tree extract 2.067 .sup. 1-2.5 Highbush cranberry (Viburnum opulus) leaf 1.1 0.7-1.3 extract Sumac plant (Rhus coriaria) extract 1.5 0.6-2.2 Jasmine (Jasminum officinale L.) 1 0.3-0.9 flower extract and/or Daisy (Anthemis nobilis) flower extract Juniper (Juniperus communis) fruit extract 3.4 .sup. 2-4.5 Apple (Malus domestica) pomace 0.98 0.74-1.2  Sunflower (Helianthus annus) face pomace 1.25 0.9-1.4 Pure silver 0.044 0.037-0.059 Pure water 88.659 86-93

[0053] Highbush cranberry plant is known for its antioxidant, antispasmodic, antiinflammatory, antiallergic, sedative and diuretic effects and is being used in the treatment of many diseases kidney diseases being at the first place. Boiled highbush cranberry can be used in the treatment of many diseases such as asthma, rheumatism, high blood pressure, epileptic episodes (epilepsy), mumps, spasms after delivery, sleep disorder. Highbush cranberry extract is also good for gall bladder disease and some liver diseases. There are also studies related to positive effects of highbush cranberry in reducing some cancer tumors.

[0054] In the composition of the invention, secondary ingredients such as valeric acid and oxalates that highbush cranberry plant comprises develops synergy with the other plant active substances that are being used together with it and reduces the synthesized nanosilver particle size to around 5-25 nm.

[0055] Myrrh tree extract has been helpful in remaining stable of the nanoparticles in the formulation. Myrrh tree consists of etheric oil in the ratio of 10 percent, resin and sticky mass in the ratio of 40 percent. Its antibacterial and antifungal effects are known.

[0056] In the composition of the invention, secondary ingredients such as limonen and mirsenol that myrrh tree extract comprises develops synergy with the other plant active substances that are being used together with it and reduces the synthesized nanosilver particle size to around 5-25 nm.

[0057] Sumac is helpful in the treatment of dermal problems and in healing of wounds with its anti-inflammatory, antibacterial and antimicrobial properties. Chewed sumac leaves are good for skin rush, gingivitis.

[0058] In the composition of the invention, sumac plant supports the antibacterial efficacy of the composition thanks to tanen and mirisetin that it comprises, and aids in reducing silver to the desired nanoparticle size.

[0059] Antimicrobial and antifungal effects of Jasmine and/or daisy flower are known, and it is used in skin replenishing.

[0060] In the composition of the invention, pleasant scent feature has been imparted to the composition thanks to addition of jasmine and/or daisy hydrosole. At the same time, humidifying feature in dermal usage has been imparted to the composition.

[0061] The resin, branches, leaves and fruits of juniper are employed in cosmetics and medicine thanks to its acidic content. Its antibacterial, antimicrobial, anti-inflamatuvar, antioxidant effects are known.

[0062] In the composition of the invention, secondary ingredients such as glycose and yunen which the juniper fruit extract comprises contribute to shortening of the time in obtaining nanosilver.

[0063] In the composition of the invention, apple pomace has imparted organic and biodegradable nanocapsule content property thanks to high cellulose and pectin that it comprises.

[0064] In the composition of the invention, sunflower face has imparted organic and biodegradable nanocapsule content property thanks to high cellulose and pectin that it comprises.

[0065] Silver is commonly used in photography industry, electronics, coin manufacturing, making ornaments and jewelries, alloys, dentistry. It is also being used in artificial raining, in making mirror tains, computer relay contacts, in making cells. Pure silver is also used in obtaining acetic acid, paints and photography substances. Likewise, silver in powder form is being used in new ceramic type coating to make glass and wood electrically conductive. Silver is a very powerful antibacterial with very low toxic. Because of this reason, it is used extensively in medical and textile fields.

[0066] In the composition of the invention, silver has been preferred as the active substance that provides antimicrobial effect to the invention. Obtaining disinfectant composition by the use of a single active substance in low amount has been achieved. Pure water is preferably at a purity of 1 microSiemens and it is effective in the formulation as a solvent. Instead of a solvent such as alcohol, acetone, etc. which is harmful and costly in terms of environment and human health, ecological and skin friendly pure water has been used as the solvent. In this manner, a product which does not cause dermal irritation and respiratory toxicity has been obtained with the composition of the invention.

[0067] The production method of the composition of the invention in its most fundamental form comprises the following process steps: [0068] i. Brewing myrrh tree, highbush cranberry leaf and sumac leaf in water, [0069] ii. Extraction of juniper fruits in water, [0070] iii. Extraction of jasmine and/or daisy plants in water/vegetative alcohol, [0071] iv. Gelling apple and sunflower face pomace in water, obtaining pectin with lactic and citric acid [0072] v. Preparation of solution with pure silver, acetic acid and hydrogen peroxide, [0073] vi. Mixing the prepared solution with the vegetative extracts obtained in process steps (i), (ii), (iii), [0074] vii. Coating the nanoparticle obtained in step (vi) with the nano-capsule material obtained in step (iv).

[0075] In the preparation of a preferred embodiment of the invention, 7 separate lightproof glass bottles having a lid are prepared. Myrrh tree plant put in lightproof bottle number 1 having a lid in the amount with which the ratios in Table 1 are satisfied is brewed in pure water first at 65-75° C. for 20-25 minutes by occasionally mixing and extract is obtained. Equal amounts of powdered highbush cranberry leaf and sumac leaf are added over the myrrh extract which has been brewed in bottle number 1 for 20-25 minutes, and it is brewed at 65-85° C. for 10-15 minutes by occasionally mixing and the obtained extract is subjected to ultrasonification process in ultrasonic device at 40° C. for 10-15 minutes for increasing the speed of extraction. At the end of the period of time, it is cooled by subjecting it to shock cooling process and is centrifuged at +1° C. at 4500-5500 rpm for 10-15 minutes. The supernatant is taken and stored at +4° C.

[0076] 10-15 grams of juniper fruits are put in lightproof bottle number 2 having a lid, and 50-80 ml of distilled water are added over it and it is brought to boiling point first at 85-95° C. for 20-30 minutes, after the extract has been subjected to ultrasonification process in the ultrasonic device for 10-15 minutes the lid of the bottle is closed and left to fermentation at 45-55° C. for 5-10 days. At the end of the period of time, the extract in bottle number 2 is centrifuged at +1° C. at 4500-5500 rpm for 10-15 minutes. The supernatant is taken and stored at +4° C.

[0077] Jasmine and/or daisy flowers put in lightproof bottle number 3 having a lid are brewed first at 35-45° C. for 5-10 minutes by adding 54% vegetative alcohol (ethanol); then brewed at 50-60° C. for 20-30 minutes by continuously mixing by adding pure water. At the same time, removal of alcohol that it comprises is achieved. The extract belonging to the mixture in bottle number 3 is cooled by subjecting it to shock cooling process in the ice bath and the cooled extract is subjected to ultrasonification process in the ultrasonic device for 10-15 minutes. The mixture cooled in bottle number 3 is centrifuged at +1° C. at 4500-5500 rpm for 5-7 minutes, is filtered through filter paper having 11 μm pore diameter and the remaining solid and liquid parts are separated.

[0078] The filtrate obtained from bottles number 1 and 2 are added to bottle number 4 in the ratio of 1.5:6.5 by volume and is mixed.

[0079] Preferably pure granule silver is weighed in the ratio of 0.037-0.059% by volume in lightproof glass bottle number 5 having a septum, and is treated with acetic acid and hydrogen peroxide in the ratio of 0.5:2 (100 ml has been taken as the baseline). In order to provide reduction of silver solution in bottle number 5 to nanosilver, 6.25-8.067 ml of plant extract are added from bottle number 4 and mixed at 750-850 rpm for 5-10 minutes. 1-2 ml of extract are taken from bottle number 3 and added to bottle number 5 and mixed for 20-30 minutes at 750-850 rpm. Acetic acid, hydrogen peroxide and alcohol are not present in the final product; alcohol is being removed prior to the synthesis, acetic acid and hydrogen peroxide are being removed during the synthesis since they are biodegradable.

[0080] 200-350 grams of Apple pomace are put in lightproof bottle number 6 having a lid, and 20-30 ml of distilled water are added over it and is smashed with a laboratory type blender for 1-3 minutes, is brought to boiling point over the hot plate by mixing continuously at 250-300 rpm at 80-90° C. for 25-30 minutes. 0.5-1.2 ml of citric acid, 0.7-1.5 ml of lactic acid are added over it and its lid is closed and mixing is continued. It is cooled at +2° C. for 20-30 minutes. It is centrifuged at 5300 rpm for 10-15 minutes and filtered through Whatman No. 1 filter paper. The filtrate is left at 60-80° C. for 20-25 minutes.

[0081] 150-250 grams of sunflower face pomace are put in lightproof bottle number 7 having a lid and 400-500 ml of distilled water are added over it and it is brought to boiling point at 90-95° C. for 35-40 minutes, 0.5-1.8 ml of citric acid, 1.2-2 ml of lactic acid are added and it is mixed continuously for 10-15 minutes at 250-350 rpm. It is smashed with a laboratory type blender for 1-3 minutes, cooled at +2° C. for 20-30 minutes. It is centrifuged at 5300 rpm for 10-15 minutes, and filtered through filter paper. The filtrate is heated at 30-40° C. for 20-25 minutes.

[0082] The supernatant in bottle number 6 is taken in the ratio of 0.74-1.2% by volume and added dropwise slowly to the mixture in bottle number 5 which is being continuously mixed at 40° C.

[0083] The supernatant in bottle number 7 is taken in the ratio of 0.9-1.4% by volume and added dropwise slowly to the mixture in bottle number 5 which is being continuously mixed at 40° C.

[0084] The product having daisy or jasmine scent, nanosilver active substance disinfectant effect coated with the obtained nanocapsule is filled in. The product is preferably supplied in spray boxes.

[0085] In the method of obtaining the composition of the invention, since all of the reducing, stabilizing agents and polymers are composed of herbal raw materials, the solution of avoiding negative results which can effect human and environmental health according to formulations with synthetic content used in prior art is proposed. Also, with the odor agents coated with nanocapsule has been proposed to prevent odor efficacy in the existing scented products getting lost in a short period of time. By using nanocapsule in the composition of the invention, enabling the active substance to be durable against varying environmental factors, and being physically more stable is being proposed. Also, enabling the capsule to be biodegradable by using organic substances instead of harmful chemicals in obtaining nanocapsule is being proposed. In order to provide this solution, Highbush cranberry leaf, Myrrh tree, Sumac leaf, Juniper fruit, Jasmine and/or daisy flower, apple and sunflower face pomace have been used in the invention.

[0086] In the method of obtaining the composition of the invention, by the synergistic effect by using Myrrh, Highbush cranberry, Juniper and Sumac extract create; [0087] reducing pure silver to 5-25 nm size has been provided; [0088] maintaining the obtained composition stable for a long time without addition of chemicals has been provided; [0089] dermal allergy has been avoided, [0090] respirable state has been achieved, [0091] production time has been shortened.

[0092] In the method of obtaining the composition of the invention, by using pure silver; [0093] active matter with antimicrobial effect has been obtained; [0094] yellow, brown, black staining problem on the applied surfaces has been eliminated; [0095] liberation of harmful chlorine and nitrate compounds in the content of the composition has been hindered.

[0096] In the method of obtaining the composition of the invention, by using jasmine or daisy saps; [0097] composition with a pleasant scent has been provided; [0098] humidifying effect in dermal application has been imparted to the composition.

[0099] In the method of obtaining the composition of the invention, by using Apple and Sunflower face pomace the following have been provided; [0100] scent giving plants preserving its efficacy for a long time with the active substance, [0101] obtaining organic origined, biodegradable coating substance, [0102] enhancing the efficacy of the composition on the surfaces it is used; [0103] composition remaining physically more stable.

[0104] Technical benefits which the disinfectant of the invention obtained as above provides superior to silver active disinfectants in the prior art are given below in Table 2.

TABLE-US-00002 TABLE 2 The properties of the invented disinfectant The composition of Silver active disinfectants the invention in prior art Silver source At 999.9 purity AgNO.sub.3 Nanoparticle size 5-25 nm 40-90 nm Dermal irritation None Present Stabilizer Plant Chemical Protectiveness on the 3-6 months A few hours surface it has been applied Active substance Silver Silver + hydogen peroxide Silver + quaternary ammonium compounds Production time 20-35 minutes after 2-8 hours according to the preparation stage the number of chemicals it has Production cost 60% less Toxicity None Present Shelf life 2 years 3 months-2 years

[0105] With the active content of the composition of the invention, antibacterial, antiviral and anti fungal effects are provided. Results of the efficacy test of the composition of the invention are given in Table 3.

TABLE-US-00003 TABLE 3 Efficacies of bacteria, mycobacteria, fungi and viruses Efficacy Log removal Action time Bacteria E. coli 99.999% 5 Log 1 minute S. aureus 99.999% 5 Log 1 minute K. pneumonia 99.999% 5 Log 1 minute E. hirae 99.999% 5 Log 1 minute P. aeruginosa 99.999% 5 Log 1 minute A. baumannii 99.999% 5 Log 1 minute Mycobacteria M. avium 99.99% 4 Log 5 minutes M. terrae 99.99% 4 Log 5 minutes Fungi A. brassiliensis 99.99% 4 Log 5 minutes C. albicans 99.99% 4 Log 5 minutes Viruses Poliovirus type 1 99.99% 4 Log 15 minutes Murine norovirus 99.99% 4 Log 15 minutes Adenovirus type 5 99.99% 4 Log 15 minutes Coronavirus Sars - CoV - 2 99.99% 4 Log 15 minutes

[0106] The composition of the invention comprises herbal synthesized silver nanoparticie in the range from 5 to 25 nm, and its BET report output is given as a chart.

Chart 1: Nanoparticles Surface Area Analysis Report (BET)

[0107] Summary Report

[0108] Surface Area [0109] Single point surface area at P/Po=0.299141472:156.2584 m.sup.2/g [0110] BET Surface Area: 154.4047 m.sup.2/g [0111] Langmuir Surface Area: 357.7420 m.sup.2/g [0112] t-Plot External Surface Area: 201.5669 m.sup.2/g [0113] BJH Adsorption cumulative surface area of pores between 17.000 A and 3000.000 A width: 128.913 m.sup.2/g Pore Volume [0114] t-Plot micropore volume: −0.019883 cm.sup.3/g [0115] BJH Adsorption cumulative volume of pores between 17.000 A and 3000.000 A width: 0.079199 cm.sup.3/g Pore Size [0116] BJH Adsorption average pore width (4V/A): 24.574 A

[0117] In this manner, the product can be used as broad effect disinfectant substance for different purposes in different fields. Herbal synthesis disinfectant composition of the invention for providing personal hygiene with general and public areas, especially the health sector being in the first place will be used: [0118] In dentistry and dental hospitals; in the disinfection of implant and prosthesis surfaces, [0119] In social areas; in the disinfection of schools, nurseries, kindergartens, sports halls, hotels, job sites and mosques; [0120] In the disinfection of mass transportation and privately owned vehicles; [0121] In homes; in providing hygiene of living areas and personal hygiene, [0122] In the disinfection of air conditioning and ventilation systems; [0123] In drug industry; against bacteria resistant to existing antibiotics, [0124] In food sector; in providing hygiene of packaging and food hygiene, [0125] In the disinfection of public area floor surfaces; [0126] In the disinfection of hospital floor surfaces; [0127] In the disinfection of intensive care room, surgery room, delivery room; [0128] In the disinfection of medical equipment; [0129] In the disinfection of public areas such as schools, mosques, municipality buildings, hospitals, subways, buses; [0130] playgrounds; In the disinfection of areas wherein children are present such as kindergartens, playgrounds, nurseries; [0131] In the disinfection of military, personal care and public areas; [0132] animal farms; to provide disinfection of animals and the workers, [0133] vehicle cleaning; in the cleaning of glasses, in removal of all kinds of dust or dirt on the wheel rim and vehicle surface, in the cleaning of seats in the car and places like ceiling, etc., [0134] food packaging and storage; in providing hygiene conditions in food packagings or storage areas; [0135] personal care; in care products such as wet wipes, diapers, soaps, hand disinfectants, tooth pastes, creams, etc.; —it prevents formation of bacteria which tend to produce in diapers or wet wipes. It prevents decay on the teeth especially when used inside tooth paste and treats the wounds inside the mouth— [0136] cosmetics; in the field of cosmetics as an antimicrobial for products such as lipsticks, blushers, eye shadows in which bacteria grow in time depending on the usage; [0137] in pool hygiene; —it is mentioned by the doctors that chlorous disinfectants trigger some health problems, and increase middle ear ailment especially in children. And in pool disinfection having biological nanoparticles such health problems are not encountered. Also, infectious diseases such as mycosis which are possible to develop in pools do not show up— [0138] sterile areas; in the sterilization of places such as hospitals and drug production areas; [0139] airlines; in the disinfection of airplanes, passenger waiting halls, ground services offices; [0140] In obtaining antimicrobial products in textile and glass industries.

[0141] In usage, for floor surface disinfection, sufficient amount of composition is sprayed, distributed to the surface with the aid of a cloth by rubbing, it does not require rinsing. And for environment disinfection, sufficient amount of product is applied by spraying or with the aid of ULV device. The environment can be used a few minutes after the application, it does not require ventilation. For tool and equipment disinfection, the materials are left inside the product which is put into the immersion container, taken out and dried a few minutes later or the product is applied over the tools and equipment in the form of direct spraying and left to dry. It does not require rinsing. It is used by adding to the finish liquor or by directly spraying and curing to the glass surface in obtaining antimicrobial effect textile and glass products. The composition of the invention can be applied as needed depending on the microbic load of the environment.