Method for producing oral composition, and oral composition

Abstract

Provided are: a method for producing an oral composition suitable for treatment or prevention of a disease or symptom in the oral cavity; and an oral composition produced by the production method. The oral composition production method characterized by including a mixing step of mixing earthworm castings with water and a collecting step of collecting vaporized water generated from a mixture obtained in the mixing step to obtain a liquid; and the oral composition produced by the production method. It is preferred to further mix an organic substance together with the earthworm castings and the water in the mixing step.

Claims

1. A method of producing an oral composition comprising: a mixing step of mixing earthworm castings with water to obtain a mixture; and a collecting step of collecting vaporized water generated from the mixture to obtain a liquid.

2. The method according to claim 1, further comprising mixing an organic substance together with the earthworm castings and the water in the mixing step.

3. The method according to claim 2, wherein the organic substance is a wood material.

4. The method according to claim 1, further comprising a diluting step of diluting the liquid with water.

5. An oral composition produced by the method according to claim 1.

6. The oral composition according to claim 5, which is a gargle or mouthwash.

7. The oral composition according to claim 5, which is for treatment or prevention of a disease or symptom in an oral cavity.

8. The oral composition according to claim 7, wherein the disease or symptom in the oral cavity is lichen planus, leukoplakia, swelling in throat, oral ulcer, halitosis, or periodontosis.

9. A method of treating lichen planus, leukoplakia, swelling in throat, oral ulcer, halitosis, or periodontosis, comprising administering the oral composition according to claim 7 to a patient.

10. The oral composition according to claim 7 for use in the treatment of lichen planus, leukoplakia, swelling in throat, oral ulcer, halitosis, or periodontosis.

11. The method according to claim 1, wherein the oral composition is a gargle or mouthwash.

12. The method according to claim 1, wherein the oral composition is for treatment or prevention of a disease or symptom in an oral cavity.

13. The method according to claim 12, wherein the disease or symptom in the oral cavity is lichen planus, leukoplakia, swelling in throat, oral ulcer, halitosis, or periodontosis.

Description

EXAMPLES

(1) Hereinbelow, the present invention will be described more specifically with reference to Examples. However, the present invention is not intended to be limited by Examples below. In Examples below, “percent (%)” is by mass unless otherwise specified.

(2) (Aqueous Organic Substance Decomposition Product)

(3) Forty liters of water was introduced into a reaction vessel containing 40 kg of earthworm Lumbricus rubellus castings and 15 kg of a wood material (Japanese cedar) (5 kg of woodchips and 10 kg of a blend of wood dusts and rice hulls), and then vaporized water was collected by a dehumidifier (DM-30, manufactured by Nakatomi Corporation) attached to the reaction vessel for about 1 day while stirring to obtain 15 to 20 L of a liquid. Ten to twenty liters of water was further added to the reaction vessel, then 10 kg of a wood material (Japanese cedar) (5 kg of woodchips and 5 kg of a blend of wood dusts and rice hulls) was added thereto, and then vaporized water was collected by the dehumidifier for about 1 day in the same manner to obtain 15 to 20 L of a liquid. About 40 L of a liquid (pH 8.71) prepared by mixing the obtained liquids was used as an aqueous organic substance decomposition product.

(4) In the production of the above-described aqueous organic substance decomposition product, the water used was water prepared by purifying tap water with an SPG (Shirasu porous glass) permeable membrane (SPG Technology Co., Ltd.) and activated carbon.

(5) (Earthworm extract)

(6) After leaving for 24 hours in a light place, 30 kg of living Lumbricus rubellus of which dirt was peeled from the skins were spread out to about 5 cm in the thickness on a flat plate, and 250 g of sodium chloride was evenly sprinkled on top of the earthworms. After 20 minutes, the earthworms were washed with water. Thereafter, 250 g of citric acid was sprinkled on the earthworms in the same manner, and then the citric acid was diluted by adding 30 liters of pure water in 15 seconds. At this time, the pH immediately after addition of water was 2.25, and the pH when completely diluted was 2.74. When sprinkled with citric acid powder, the earthworms released yellow body fluid at once. After the dilution of the citric acid with water, the earthworms were kept for 20 minutes in that state. Subsequently, the living earthworms were taken out from the soiled citric acid aqueous solution, washed with water, and milled at 10° C. using a homogenizer to prepare an earthworm paste. Next, after sucking and deaerating the earthworm paste to remove a gas contained therein, the earthworm paste was then placed on a stainless steel tray, instantaneously cooled to −35° C., and gradually frozen for 50 hours while maintaining this temperature. The frozen earthworm paste was kept at −35° C. and 0 Pa for 2 hours, the temperature was then raised to 25° C. at 40 Pa for 10 hours, the temperature was then raised to 40° C. at 35 Pa for 14 hours, the temperature was then raised to 65° C. at 35 Pa for 12 hours, and finally, the temperature was raised to 80° C. at 25 Pa for 6 hours to perform vacuum freeze drying. By this treatment, light yellow dry earthworm powder having a water content of 8% by mass was obtained.

(7) 25 g of the dry earthworm powder obtained as above was sampled, 500 mL of distilled water was added thereto, and the mixture was stirred at room temperature for 1 hour and extracted. The obtained extract was centrifuged (10,000×g, 4° C., 15 minutes), and a supernatant was collected to obtain an earthworm extract. The obtained earthworm extract was finely pulverized with a freeze vacuum dryer, sampled by 30 mg, and diluted with 500 mL of distilled water to obtain a stock solution.

(8) (Oral Composition)

(9) The aqueous organic substance decomposition product obtained above was diluted 5 times with water. The water used for dilution was water prepared by purifying tap water with an SPG (Shirasu porous glass) permeable membrane (SPG Technology Co., Ltd.) and activated carbon.

(10) The diluted aqueous organic substance decomposition product and the diluted stock solution of the earthworm extract obtained above were mixed at a ratio of 1,000:1 and passed through a 0.2 μm filter to obtain an oral composition.

Example 1

(11) <Lichen Planus>

(12) The symptoms of lichen planus of the subject below who underwent gargling 5 to 6 times per day on a daily basis with the oral composition obtained above were observed.

(13) Subject: Male, 65 years old, onset in 2012, no administration of immune enhancing agent.

(14) After 10 days, the roughness of the gums slightly resolved; and the color remained unchanged, milky white.

(15) After 30 days, the roughness of the gums disappeared; and the milky white became thin.

(16) After 60 days, the gums became smooth; and the milky white became considerably thin.

(17) After 90 days, the gum state was normal including color.

(18) After 180 days, no abnormality was found.

(19) One year later, no abnormality was found.

(20) Two years later, no abnormality was found.

(21) As of Dec. 20, 2016, no abnormality was found.

Example 2

(22) <Leukoplakia>

(23) The symptoms of leukoplakia of the subject below who underwent gargling 5 to 6 times per day on a daily basis with the oral composition obtained above were observed.

(24) Subject: Female, 60 years old, onset in 2013, no medication treatment.

(25) After 10 days, the color of the gums was changed.

(26) After 20 days, the color became a bit thinner.

(27) After 30 days, the color became considerably thin.

(28) After 60 days, gum color became normal.

(29) As of Mar. 20, 2016, No abnormality was found.

Example 3

(30) <Swelling in Throat 1>

(31) The symptoms of swelling in throat of the subject below who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(32) Subject: Male, 18 years old.

(33) Pain disappeared 30 minutes after gargling.

(34) After 7 days, swelling subsided.

Example 4

(35) <Swelling in Throat 2>

(36) The symptoms of swelling in throat of the subject below who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(37) Subject: Female, 35 years old.

(38) Pain disappeared 20 minutes after gargling.

(39) After 9 days, swelling subsided.

Example 5

(40) <Oral Ulcer 1>

(41) The symptoms of oral ulcer of the subject who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(42) Subject: Female, 30 years old.

(43) After 3 days, the pain disappeared.

(44) After 10 days, the shape of the ulcer disappeared.

(45) After 15 days, completely cured.

Example 6

(46) <Oral Ulcer 2>

(47) The symptoms of oral ulcer of the subject below who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(48) Subject: Male, 56 years old, ulcer made by injuring with teeth.

(49) After 2 days, the pain disappeared.

(50) After 7 days, the dent of the ulcer became shallow

(51) After 15 days, the shape of the ulcer disappeared

(52) After 20 days, completely cured.

Example 7

(53) <Periodontosis 1>

(54) The symptoms of periodontosis of the subject below who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(55) Subject: Female, 40 years old, bad breath.

(56) After 3 days, halitosis decreased.

(57) After 7 days, halitosis disappeared.

(58) After 14 days, gum pain decreased.

(59) After 20 days, the red color of the gums became thin.

(60) After 30 days, the red color disappeared.

(61) After 45 days, the gums became normal

Example 8

(62) <Periodontosis 2>

(63) The symptoms of periodontosis of the subject who underwent gargling 5 to 6 times a day on a daily basis with the oral composition obtained above were observed.

(64) Subject: Male, 35 years old, bad breath.

(65) After 1 day, halitosis decreased.

(66) After 5 days, halitosis disappeared.

(67) After 10 days, swelling of the gums subsided.

(68) After 15 days, the red color of the gums disappeared.

(69) After 30 days, the gums became normal.

(70) As shown in Examples 1 to 8, it was confirmed that an oral composition suitable for treating or preventing a disease or symptom in the oral cavity can be produced by collecting evaporated water generated from a mixture of earthworm castings and water.