Controlled release delivery system for nasal application of neurotransmitters

Abstract

This invention relates to a galenical gel formulation for nasal administration of neurotransmitters/neuromodulators such as dopamine, serotonin or pregnenolone and progesterone. The special lipophilic or partly lipophilic system of the invention leads to high bioavailability of the active ingredient in plasma and brain caused by sustained serum levels and/or direct or partly direct transport from nose to the brain.

Claims

1. A dopamine gel formulation for nasal administration comprising: (a) dopamine in an amount of from about 0.01% to about 6% by weight of the dopamine gel formulation; (b) castor oil in an amount of from 75% to 95% by weight of the dopamine gel formulation; (c) oleoyl macrogolglyceride or a mixture of oleoyl macrogolglycerides in an amount of from 0.5% to about 20% by weight of the dopamine gel formulation; and (d) colloidal silicon dioxide in an amount of from 0.5% to 10% by weight of the dopamine gel formulation.

2. The dopamine gel formulation according to claim 1, wherein said dopamine is present in an amount of from 0.5% to 4% by weight of the dopamine gel formulation.

3. The dopamine gel formulation according to claim 1, wherein said castor oil is present in the dopamine gel formulation in an amount of from 85% to 95% by weight of the dopamine gel formulation.

4. The dopamine gel formulation according to claim 1, wherein said oleoyl macrogolglyceride or a mixture of oleoyl macrogolglycerides is present in an amount of from 1% to 20% by weight of the dopamine gel formulation.

5. The dopamine gel formulation according to claim 1, further comprising at least one surfactant selected from the group consisting of lecithin, a fatty acid ester, at least one humectant, and mixtures thereof.

6. The dopamine gel formulation according to claim 1, wherein said colloidal silicon dioxide is present in an amount of from 2% to 5% by weight of the dopamine gel formulation.

7. The dopamine gel formulation according to claim 1, further comprising a thickener or gelling agent selected from the group consisting of cellulose, cellulose derivatives, polysaccharides, carbomers, polyvinyl alcohol, povidone, colloidal silicon dioxide, cetyl alcohols, stearic acid, beeswax, petrolatum, triglycerides, lanolin and mixtures thereof.

8. The dopamine gel formulation according to claim 1, wherein said dopamine is in the form of microspheres.

9. The dopamine gel formulation according to claim 1, wherein said dopamine is in the form of liposomes.

10. A dopamine gel formulation comprising: (a) dopamine in an amount of about 2% by weight of the formulation; (b) castor oil in an amount of about 90% by weight of the formulation; (c) an oleoyl macrogolglyceride or a mixture of oleoyl macrogolglycerides in an amount of about 4% by weight of the formulation; and (d) colloidal silicon dioxide in an amount of about 4% by weight of the formulation.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) The following detailed description, given by way of example, but not intended to limit the invention solely to the specific embodiments described, may best be understood in conjunction with the accompanying drawings, in which:

(2) FIG. 1 shows the effect of nasal dopamine gel on immobility and climbing of depressed rats.

(3) FIG. 2 shows the concentration of dopamine and metabolites in nucleus accumbens and neostriatum after nasal administration of dopamine.

DETAILED DESCRIPTION

(4) The galenical gel formulation of the presently claimed invention is chemically and physically stable and can be in the form of a suspension or a solution of the pharmacologically active substance. In a preferred embodiment, the galenical gel formulation of the invention is filled into a preservative-free device able to accurately deliver doses of the above formulation, even at a high viscosity.

(5) After nasal application of the galenical gel formulation, the active ingredient or active ingredient particles are typically efficiently trapped at the deposition site and are typically absorbed at a predictable rate across the mucous membrane of the patient, thereby limiting possible deactivation by metabolizing enzymes and/or protein-binding.

(6) It is also understood that the terms and expressions used herein have the ordinary meaning as is accorded to such terms and expressions with respect to their corresponding respective areas of inquiry and study except where specific meanings have otherwise been set forth herein.

(7) The term “higher availability” or “higher bioavailability” shall mean that after application of the active ingredient(s), significant and constant in vivo therapeutic drug concentrations, especially in the brain, are maintained for an extended period of time.

(8) The composition of the invention may comprise a neurotransmitter and/or neuromodulator agent. A neurotransmitter, as used herein, may be a molecule used for signaling between nerve cells or neurons. Neurotransmitter molecules typically pass between neurons at synapses, and may causes a rapid, short-lived and dramatic response. Neurotransmitters may degrade rapidly, resulting in restoration of the resting membrane potential. Examples include but are not limited to Acetylcholine, Monoamines (epinephrine, norepinephrine, dopamine, serotonin, melatonin, histamine), Amino acids (glutamate, gamma aminobutyric acid (GABA), aspartate, glycine), Purines (Adenosine, ATP, GTP, and their derivatives), neuroactive peptides (Substance P. N-Acetylaspartylglutamate), Neurohypophyseal transmitters (Oxytocin, Vasopressin), Opioids (Endorphin), zinc, nitric oxide, carbon monoxide, and chemical modifications of such molecules, such as esters.

(9) Neurotransmitters such as dopamine, serotonin, epinephrine and norepinephrine contain the catechol moiety typically synthesized from the amino acid tyrosine.

(10) One neurotransmitter commonly associated with any number of responses is dopamine or 4-(2-aminoethyl)-1,2-benzenediol. Dopamine is commonly regarded as an endogenous catecholamine with α and β adrenergic activity.

(11) L-DOPA is commonly synthesized from aminotyramine and converted into dopamine in the brain. Other neurotransmitters are typically contained in synaptic vesicles in terminals of the presynaptic neuron, and upon stimulation of an action potential, are released into the synaptic cleft to induce a response in the post synaptic cell. Iontophoresis of the neurotransmitter into a synaptic cleft induces the same postsynaptic response. The neurotransmitter then degrades rapidly resulting in restoration of the resting potential.

(12) Other catecholamines known to have various utilities include epinephrine, norepinephrine, and serotonin.

(13) A neuromodulator agent, as used herein, may be a substance other than a neurotransmitter, released by a neuron at a synapse and conveying information to adjacent or distant neurons, either enhancing or dampening their activities. Neuromodulation is the process in which several classes of neurotransmitters in the nervous system regulate diverse populations of neurons. Neuromodulators may act not only on the input system but may change the transformation itself to produce the proper contractions of muscles as output. Neuromodulator agents may also include neurosteroids. A variety of steroids are synthesized in the central and peripheral nervous system, especially in myelinating glial cells, from cholesterol or steroidal precursors imported from peripheral sources. Such steroids are defined as neurosteroids. Neurosteroids may rapidly alter neuronal excitability through interaction with neurotransmitter-gated ion channels. In addition, neurosteroids may also exert effects on gene expression via intracellular steroid hormone receptors. Examples include but are not limited to pregnenolone, dehydroepiandrosterone, their sulfates, and reduced metabolites such as the tetrahydroderivative of progesterone 3α-hydroxy-5α-pregnane-20-one. Neuromodulator agents also include chemical modifications of neurosteroids, such as esters and synthetic neurosteroids such as minaxolone, ganaxolone.

(14) In a preferred embodiment, the active ingredient may comprise 0.01 to 6% by weight, preferably 0.1 to 4% by weight, more preferably 0.5 to 2% by weight, and most preferably at around 2% by weight of the formulation.

(15) The carrier of the invention functions to dissolve or suspend the drug and otherwise support the various constituents of the invention. To that end, the carrier has a lipophilic nature or character. Suitable families of oils include fatty acids and oils such as mineral and vegetable oils, in particular, fatty acids and oils derived from vegetable stock are especially useful. Both linear and branched chain acids and oils are useful including those oils and acids with various levels of saturation and substitution. Chain size including but is not limited to formic, acetic, propionic, butyric, valeric, caproic, emanthic, caprylic, pelargonic, capric, laurie, myristic, palmitic and stearic, among others, all represent useful oil and acid moieties.

(16) The term “lipophilic carrier” may comprise, but is not limited to, fats and/or vegetable oil such as castor oil, soybean oil, sesame oil, or peanut oil, fatty acid esters such as ethyl- and oleyloleat, isopropylmyristate, medium chain triglycerides, glycerol esters of fatty acids, or polyethylene glycol, phospholipids, white soft paraffin, or hydrogenated castor oil, or a mixture thereof. In one embodiment, the active ingredient may also be incorporated into an oil mixture. In a preferred embodiment, the vegetable oil is castor oil.

(17) The particular amount of lipophilic carrier that constitutes an effective amount is dependent on the particular viscosity regulating agent used in the formulation. It is therefore not practical to enumerate specific amounts for use with specific formulations of the invention.

(18) In a preferred embodiment, the lipophilic carrier may comprise between 30% and 98% by weight, preferably between 60 and 98% by weight, more preferably between 75% and 95% by weight, even more preferably between 85% and 95% by weight, and most preferably around 90% by weight of the formulation.

(19) The surface active agent or surfactant of the invention functions to decrease surface tension in the composition of the invention. Surfactants are generally regarded as those compositions which have both hydrophilic and lypophilic character. The lypophilic character of the surfactant typically takes the form of a pendent moiety having little if any charge. In turn, the hydrophilic character of the surfactant is typically charged and dictates the class into which the surfactant is identified. For example, surfactants typically used in the invention include nonionic surfactants, anionic surfactants, amphoteric surfactants, and cationic surfactants.

(20) In one embodiment, component (c), a compound or a mixture of compounds having surface tension decreasing activity, may comprise at least a surfactant including, but not limited to, lecithin, fatty acid ester of polyvalent alcohols, fatty acid ester of sorbitanes, fatty acid ester of polyoxyethylensorbitans, fatty acid ester of polyoxyethylene, fatty acid ester of sucrose, fatty acid ester of polyglycerol, and/or at least one humectant such as sorbitol, glycerine, polyethylene glycol, or macrogol glycerol fatty acid ester. Particularly useful, however, are oleoyl macrogolglycerides (such as LABRAFIL® 1944 CS, as available from Gattefosse (Saint-Priest, France)).

(21) As defined herein, “surface tension decreasing activity” shall mean having a surface tension that results in generating contact of the formulation to the hydrophilic mucous membranes of the nasal cavity.

(22) In one embodiment, the active ingredient/neurotransmitter may be incorporated into a surfactant mixture. The particular amount of surfactant that constitutes an effective amount is dependent on the particular oil or oil mixture used in the formulation. It is therefore not practical to enumerate specific amounts for use with specific formulations of the invention. Typically, however, the surfactant may comprise 1 to 20% by weight, preferably 1 to 10% by weight, more preferably 1 to 5% by weight, and most preferably at around 4% by weight of the formulation.

(23) The term “viscosity-regulating agent” shall mean a thickener or gelling agent.

(24) Examples of a viscosity-regulating agent include, but are not limited to, cellulose and derivatives thereof, polysaccharides, carbomers, polyvinyl alcohol, povidone, colloidal silicon dioxide, cetyl alcohols, stearic acid, beeswax, petrolatum, triglycerides, lanolin, or the like. A preferred viscosity regulating agent is colloidal silicon dioxide (such as AEROSILO®, as available from Degussa).

(25) The incorporation of the active ingredient is also possible into a mixture of thickeners or gelling agents. The particular amount of thickener/gelling agent that constitutes an effective amount is dependent on the particular oil or oil mixture used in the formulation. It is therefore not practical to enumerate specific amounts for use with specific formulations of the invention. Typically, however, the thickener/gelling agent(s) may comprise 0.5 to 10% by weight, preferably 0.5 to 5% by weight, more preferably 2 to 5% by weight, and most preferably at around 4% by weight of the formulation.

(26) Preferred weight percentages for components of the galenical gel formulation are shown in Table 1.

(27) TABLE-US-00001 TABLE 1 Preferred weight percentages components of the galenical gel formulation Wt-% Useful Preferred More Preferred Active Ingredient 0.01 to 6   2 to 4 0.5 to 2   (e.g. Neurotransmitter/ Neuromodulator agent Lipophilic Carrier 30 to 98 60 to 98 85 to 95 Surfactant  1 to 20  1 to 10 1 to 5 Viscosity Regulating 0.5 to 10  0.5 to 5   2 to 5 Agent

(28) Generally, the galenical formulation of the invention can be prepared easily by the following conventional method:

(29) The lipophilic carrier and surfactant are filled into a stirrer vessel and about 75% of the viscosity regulating agent is mixed in. The active ingredient is added while stirring to obtain a homogenous dispersion of the active ingredient. Next, the formulation is adjusted to the necessary viscosity with the remainder of the viscosity regulating agent.

(30) In a preferred embodiment, the formulation may be filled into a preservative-free container.

(31) Because the active ingredient, particularly a neurotransmitter, may have lower levels of solubility in water, release from the formulation is the rate-limiting step for adsorption. The presently claimed invention demonstrates that the incorporation of the active ingredient into an oily formulation containing a suitable surfactant leads to physiologically clinically effective serum and brain levels and to an appropriate sustained action of the active ingredient over time.

(32) This sustained and clinically favorable release of the active ingredient is due to the interaction of the compounds in the oily carrier, which remains on the mucous membrane of the nasal cavity for a prolonged duration of time.

(33) The active ingredient of this invention may be introduced into the formulation also in a processed form, such as microspheres, liposomes, among others.

(34) The formulation according to this invention may also be processed into powder form, such as by lyophilization or spray-drying.

(35) The most preferred formulation of the presently claimed invention is presented in Table 2.

(36) TABLE-US-00002 TABLE 2 Most Preferred formulation Compound Amount per container Delivery per spray Dopamine/L-DOPA 2% ~2.8 mg Colloidal silicon dioxide 4% ~4.2 mg Oleoyl macrogol-glycerides 4% ~5.6 mg Castor Oil 90% ~127.4 mg 

(37) In one embodiment, the formulation is administered to a patient. In a preferred embodiment, the formulation is administered to a human.

(38) When administered to a patient, the formulation that optionally comprises a pharmaceutically acceptable vehicle may be administered by absorption through mucocutaneous linings and may be administered together with another biologically active agent. Administration may be local.

(39) In a specific embodiment, the term “pharmaceutically acceptable” means approved by a regulatory agency of the Federal or a state government or listed in the U.S. Pharmacopeia or other generally recognized pharmacopeia or otherwise proven as safe for use in animals, mammals, and more particularly in humans. The term “vehicle” refers to a diluent, adjuvant, excipient, or carrier with which a compound of the invention is administered. Such pharmaceutical vehicles can be lipophilic semisolids or liquids such as oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like.

(40) The amount of the formulation that will be effective in the treatment of a particular disease will depend on the nature of the disease, and can be determined by standard clinical techniques. In addition, in vitro or in vivo assays may optionally be employed to help identify optimal dosage ranges. The precise dose to be employed will also depend on the seriousness of the disease, and should be decided according to the judgment of the practitioner and each patient's circumstances.

(41) Recommended dosages for administration by inhalation are in the range of about 0.001 milligram to about 200 milligrams per kilogram of body weight per day. Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems. Such animal models and systems are well known in the art.

(42) The formulation is preferably assayed in vitro and in vivo, for the desired therapeutic or prophylactic activity, prior to use in humans. Animal model systems can be used to demonstrate safety and efficacy.

EXAMPLES

(43) The invention will now be further described by way of the following non-limiting examples.

Example 1

Nasal Administration of Dopamine to Rats

(44) A dopamine (DA) gel of the inventive formulation was nasally administered to rats used in the validated “forced swimming test.” As shown in FIG. 1, the administration of dopamine results in anti-depressive-like effects. As shown in FIG. 2, strong dopaminergic activity in the neostriatum and ventral striatum (nucleus accumbens) was observed after nasal application of dopamine with the inventive formulation.

(45) Generally, antidepressants must be administered for an extended length of time before antidepressive effects are observed. Surprisingly, after nasal application to rat of the dopamine gel formulation, antidepressive effects occurred within hours and without any observable side effects, such as those side effects known to occur with desipramine (apathy) or fluoxetine (weight loss).

(46) After nasal application to rats of dopamine in the inventive gel formulation, the concentration of dopamine in the nucleus accumbens and neostriatum level increased rapidly by more than 1000 percent. These results differ from those previously described. After nasal application of an aqueous dopamine solution to mice, Bjorn Jansson, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 305 (2004), found dopamine in the olfactory bulb but the compound peaked after four hours. After nasal application to rats of aqueous dopamine solution, Maria Dahlin, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Pharmacy 240 (2000), found dopamine in CSF after a short time but the increase of the compound from baseline was much lower than with the nasal gel of the invention. Ikeda et al., Chem. Pharm. Bull. 40(8): 2155-2158 (1992), increased bioavailability of nasally-given dopamine by some degree using the excipients hydroxypropyl cellulose (HPC) and Azone (1-dodecylazacycloheptan-2-one), respectively. De Souza Silva et al., Synapse 27:294-302 (1997), showed that by nasally applying to rats an aqueous L-DOPA methyl ester solution (50 mg/kg), the dopamine level in the neostriatum were increased by about 130%. The metabolites 3,4-dihydroxyphenyl acetic acid (DOPAC) and homovanillic acid (HVA) slightly increased by contrast to what was seen after intraperitoneal (IP) application of L-DOPA methyl ester in De Souza Silva et al., J. Neurochem. 68(1): 233-239 (1997).

(47) Furthermore, the metabolism of dopamine appears to be quite different than that previously described. Unexpectedly, as shown in FIG. 2, dopamine in the CSF was not metabolized to 3,4-dihydroxyphenyl acetic acid (DOPAC) or homovanillic acid (HVA) as is typically seen. These results demonstrate that nasal application of dopamine in the inventive gel formulation may be useful for the treatment of diseases associated with dopamine deficiency in the brain, such as Parkinson's disease, attention deficit hyperactivity disorder (ADHD), and addiction to drugs and/or alcohol.

(48) The features disclosed in the foregoing description, in the claims and/or in the drawings may, both separately and in any combination thereof, be material for realizing the invention in diverse forms thereof.

(49) Having thus described in detail preferred embodiments of the present invention, it is to be understood that the invention defined by the above paragraphs is not to be limited to particular details set forth in the above description as many apparent variations thereof are possible without departing from the spirit or scope of the present invention.