Positive displacement pipette having an improved ejection function

Abstract

A positive displacement sampling pipette which includes a control rod, the bottom end of which controls the displacement of a device for gripping the top end of a piston of a capillary-piston assembly intended to cooperate with said pipette. The pipette includes an ejection rod of the capillary-piston assembly, movably mounted with respect to the control rod such that its bottom end exerts an ejection strain on the top end of the piston accommodated in the gripping device during a relative displacement between the ejection rod and the control rod.

Claims

1. A positive displacement sampling pipette, comprising: a control rod having a bottom end which controls a displacement of a device for gripping a top end of a piston belonging to a capillary-piston assembly adapted to cooperate with said pipette, wherein said pipette includes an ejection rod for ejecting said capillary-piston assembly, and wherein said ejection rod is movably disposed within said control rod such that a bottom end of the ejection rod exerts an ejection strain on the top end of said piston accommodated in said gripping device during a relative displacement between the ejection rod and the control rod.

2. The pipette according to claim 1, wherein the control rod is hollow and said ejection rod is accommodated inside the hollow control rod.

3. The pipette according to claim 2, further comprising a control button arranged at the top end of the control rod, as well as an ejection button arranged at the top end of the ejection rod.

4. The pipette according to claim 3, wherein the ejection button is arranged between the control button and a handle forming a pipette body.

5. The pipette according to claim 4, wherein said ejection button is carried by a support member mounted on the ejection rod and passing through an oblong passageway provided in the control rod, said support member being able to slide in said oblong passageway.

6. The pipette according to claim 1, wherein the gripping device has a form of a clip having at least two jaws biased into a clamped position by elastic return means.

7. The pipette according to claim 6, wherein the elastic return means associated with the at least two jaws surround the at least two jaws.

8. The pipette according to claim 1, further comprising elastic return means biased to retain said ejection rod into a high position relative to said control rod.

9. The pipette according to claim 8, further comprising: a control button arranged at the top end of the control rod, as well as an ejection button arranged at the top end of the ejection rod, wherein said elastic return means which bias said ejection rod into the high position relative to said control rod are designed such that upon actuating said ejection button, a first stroke results in driving said control rod with said ejection rod, via said elastic return means, and wherein a second stroke is triggered when the displacement of the control rod is translationally stopped by means of a stop at the end of said first stroke, said second stroke resulting in said relative displacement between the ejection rod and the control rod, thus causing said ejection strain on the top end of said piston.

10. The pipette according to claim 9, wherein at an end of said first stroke, said control rod is stopped to place said gripping device at least partially projecting downwardly from a pipette tip on which the capillary of said capillary-piston assembly is adapted to be fitted.

11. The pipette according to claim 10, wherein a bottom stop for said control rod is arranged on a low part of a pipette integrating the tip, and cooperates with a stop provided on said gripping device interposed between said control rod and the bottom stop.

12. The pipette according to claim 1, further comprising a device for adjusting a sample volume to be sampled.

13. A pipetting method using a positive displacement sampling pipette including a control rod having a bottom end which controls a displacement of a device for gripping a top end of a piston belonging to a capillary-piston assembly adapted to cooperate with said pipette, the pipette including an ejection rod for ejecting said capillary-piston assembly, said ejection rod being movably disposed within said control rod such that a bottom end of the ejection rod exerts an ejection strain on the top end of said piston accommodated in said gripping device during a relative displacement between the ejection rod and the control rod, and the gripping device having a form of a clip having at least two radially outwardly-biased jaws biased into a clamped position by elastic return means, the pipetting method comprising the following successive steps: (a) fitting a capillary of the capillary-piston assembly onto a tip of the pipette, and introducing the top end of the piston of the assembly into the clip during the displacement of said control rod into a low position, said introducing being performed by moving apart the at least two radially outwardly biased jaws by bearing the top end of the piston, said moving apart being allowed by deforming said elastic return means associated with said at least two radially outwardly biased jaws; (b) sampling and dispensing a sample by actuating said control rod; and (c) ejecting the capillary-piston assembly by actuating said ejection rod.

14. The method according to claim 13, further comprising at the end of step (a), keeping said control rod in the low position until the sample is sampled.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

(1) This description will be made with regard to the appended drawings wherein:

(2) FIG. 1 represents a longitudinal cross-section view of a positive displacement sampling pipette, according to a preferred embodiment of the present invention;

(3) FIG. 2 represents a front view of the control rod equipping the pipette shown in the preceding figure;

(4) FIG. 3 represents a perspective view of the piston clip equipping the pipette shown in FIG. 1;

(5) FIG. 4 represents a longitudinal cross-section partial view of the clip shown in the preceding figure; and

(6) FIGS. 5 to 8 represent different views schematically representing the operation of the pipette shown in the preceding figures.

DETAILED DISCLOSURE OF PREFERRED EMBODIMENTS

(7) First, in reference to FIG. 1, a positive displacement sampling pipette 1 is represented according to a preferred embodiment of the present invention.

(8) Throughout the description that follows, the terms “top” and “bottom” are to be considered with the pipette kept vertically, in a pipetting position or close to this same position.

(9) The pipette 1 has an outer body the top part of which forms a handle 2 for the operator, and the bottom part 4 of which is more tapered, ending downwardly with a tip 6 on which a capillary is intended to be fitted. The bottom part 4 is preferentially mounted screwed on the handle forming body 2, so as to facilitate assembly/disassembly.

(10) The pipette integrates a control rod 8, slidably accommodated inside the pipette outer body. The rod 8 is hollow, and arranged along the longitudinal axis 10 of the pipette. Its top end projects upwardly from the handle forming body 2, and carries a control button 12 intended to be actuated by an operator's thumb holding the body 2 with one of his/her hands. As is shown in FIG. 2, the rod has a non-circular shaped transverse cross-section, herein a hexagonal or orthogonal shape. It is slidably accommodated through a screw 14 for adjusting the volume to be sampled, the inner hollow part of which is of a complementary form to the outer surface of the rod 8, and the outer surface of which is threaded, screwedly mounted to the lower end of the handle forming body 2.

(11) In a known manner, the rotation of the control rod 8 by its button 12 enables the adjusting screw to be displaced relative to the pipette outer body along the direction of the axis 10, and thus results in a change in a sample volume intended to be sampled.

(12) The bottom end of the control rod 8, acting as a guide in the bore 18 provided within the low part 4, is axially bearing against a clip 20 also slidably mounted in the bore 18.

(13) As can be seen in FIGS. 1, 2 and 4, the clip 20 includes a body 24 for contacting the bottom end 16 of the control rod, extended downwardly by two or more jaws. By way of indicative example, two jaws 26 are provided, one made as a single piece with the clip body 24, and the other mounted to this same clip body. Elastic return means enable both jaws to be returned into a clamped position, wherein they are radially inwardly retracted. The slip 30 separating them then has a low dimension.

(14) To do this, the elastic return means bias the jaws 26 radially inwardly, preferably by surrounding the same jaws. This can thus be a spring 32 having a general annular shape, the diameter of which can be increased when it is radially outwardly stressed. In the example shown in the figures, the spring 32 takes the shape of a spiral spring encircling the external surface of the jaws 26.

(15) As can be better seen in FIG. 4, each jaw 26 has a chamfered end 36 so as to facilitate insertion of the piston, as will be explained hereinafter.

(16) Furthermore, the clip body 24 has a first shoulder 38 directed downwardly, facing away a shoulder 40 provided on the bottom part 4, in proximity of the tip 6. A return spring 42 is accommodated bearing between these two shoulders 38, 40, in order to make up a return spring at the high position of this clip 20 and of the control rod bearing against it, extending therefrom. The return force developed by this compression spring 42 effectively causes the control rod 8 to assume its high position with respect to the outer body, a conventional high stop (not shown) being provided to that end on this same outer body.

(17) The clip 20 includes a second shoulder 44, also directed downwardly facing away the shoulder 40. The second shoulder 44 is located lower and radially inwardly with respect to the first shoulder 38. It is thus surrounded by the spring 42. As will be described hereinafter, it is provided to make up a low stop for the clip 20 and the control rod 8 bearing against this same clip. In the high position shown in FIG. 1, the ends 36 of the clip jaws are located in the tip 6, in a retracted position inside the same. The inside diameter of the tip has on the other hand a value close to the average diameter of the clip 20 with its jaws 26 in a clamped position.

(18) One of the features of the present invention lies in the presence of an ejection rod 46 slidably mounted inside the hollow control rod 8. This ejection rod 46 has an external surface complementary to the internal surface of the control rod 8. Its top end is arranged between the control button 12 and the handle forming body 2, and carries an ejection button 48. To do this, the button 48 is carried by a support member 50 having a pin shape, which is mounted to the ejection rod 8 and which passes through an oblong passageway 52 provided in the control rod, shown in FIG. 2. The pin 50 is then able to slide in the oblong passageway 52 during the relative displacement between both rods 8, 46, corresponding to a sliding along the direction of the axis 10.

(19) The bottom end 54 of the ejection rod 46 is more tapered, having a cylindrical shape and circular cross-section. It enters within a bore 58 provided within the hollow body, passing through up the space 60 defined between the clips.

(20) At the shape change between the bottom end 54 and the high part of the rod 46, this defines a shoulder 66 directed downwardly, facing away a shoulder 68 provided on the clip 20, in the bore 58. A return spring 64 is accommodated bearing between these two shoulders 66, 68, in order to make up a return spring in a high position for the ejection rod 46 with respect to the control rod 8 located outwardly. The return force developed by this compression spring 64 effectively causes the ejection rod 46 to assume its high position with respect to the control rod 8, a high stop being made by pressing the ejection button surrounding the rod 8, on the bottom end of the control button 12, as can be seen in FIG. 1. Such a stop could alternatively being made by pressing the pin 50 onto the top end of the oblong passageway 52 provided transversely on the control rod.

(21) In reference now to FIGS. 5 to 8, the operation of the pipette 1 will be described.

(22) First, the operator gripping the pipette through the handle 2 engages the tip 6 in a capillary 80 of a capillary-piston item assembly 84, preferably arranged in a case, also called “rack”. By exerting a vertical pressure downwardly onto the pipette, he/she achieves the fitting of the capillary 80 on the tip 6, much like fitting a capillary or a conventional cone onto the tip of a conventional air displacement pipette. This sleeving has been schematically represented in FIG. 5.

(23) Then, as can be seen in FIG. 6, the operator presses the control button 12 in order to bring the control rod and the clip into a low position. The object of this displacement is to lead to the introduction of the top end 86 of the piston 82 of the assembly 84 into the clip 24. This piston introduction will be detailed below.

(24) During a descent of the rod 8 counteracting the return force of the spring 42, the chamfered ends 36 of the clip jaws start with contacting the end 86 of the piston 82, that they push accordingly into a low position in the capillary 80 if not already made. This low position is reached when the flange 90 located under the top end 86 of the piston 82 abuts against a corresponding shoulder 92 inside the capillary 80.

(25) Then, as the descent goes on, the jaws 36 are radially outwardly biased by the top end of the piston 86 bearing against the chamfered ends 36 of these jaws. This results in the jaws being moved apart, which is allowed by the stressing of the spiral spring 32 surrounding these jaws, and permitted by a sufficient bore diameter in the tip 6. Besides, during this descent, a low part of the jaws is intended to project from the tip 6 so as to enable these jaws to be more radially outwardly deformed.

(26) When the jaws move apart and the descent of the control rod proceeds, this leads to gradually introduce the top end 86 of the piston into the space 60 defined by these jaws. The introduction is completed when the ends 36 of the jaws abut against the flange 90 of the piston, in turn abutting against the shoulder 92, as is shown in FIG. 6. This position corresponds above all to the low position of the control rod and of the clip bearing against this rod, since the clip 20 is then in a low stop on the lower body 4, by contact between the shoulders 44, 40. At this stage, the spring 42 is compressed to the maximum between both shoulders 38, 40.

(27) At the end of this step, the piston is located at the low stop position in the capillary. Thus, to simplify the sampling process, the control rod 8 is kept in a low position until the sample is sampled, during which the control rod raises with the piston to cause the liquid suction.

(28) The dispensing of the liquid sample is then performed, by displacing the control rod via its button 12, in an identical way as that performed for gripping the piston. Indeed, the stroke is the same, bringing the control rod 8 into a low position up to the contact between the shoulders 44, 40, shown in FIG. 6.

(29) Finally, the ejection of the item assembly 84 is carried out, using the ejection rod 46 actuated by its control button 48.

(30) The stiffness of the return spring 64 is such that during a first stroke, this spring is not compressed and the ejection rod 46 being displaced drives the control rod 8 with it. This phase has been schematically represented in FIG. 7.

(31) When the control rod 8 is displaced and the clip 20 is translationally stopped by means of a stop of the shoulders 44, 40 and that the button 48 continues to be actuated downwardly, then a second stroke of the ejection rod occurs, during which there is a relative displacement of the rod 46 with respect to the rod 8 staying fixed. This results in bringing the bottom end 54 of the rod 46 in contact with the top end 86 of the piston 82, and then in exerting an ejection effort on this same top end of said piston. The rod 46 thus drives off the piston 82 which, by bearing via its flange 90, drives the capillary 80 with it until the item assembly 84 is ejected, as has been schematically represented in FIG. 8. In this regard, the shape of the end 54 of the ejection rod 46 can be optimized to best fit snugly the top end 86 of the piston 82, so as to promote contact between both pieces, by centring.

(32) Of course, various changes can be made by those skilled in the art to the invention just described, only by way of non-limiting examples.