CHIP CARRIER FOR SINGLE SPERM OR EXTREMELY SMALL AMOUNT OF SPERMS

Abstract

The invention discloses a chip carrier for single sperm or extremely small amount of sperms, comprising a chip carrier, an X indicator axis, a Y indicator axis, and microarrays; the chip carrier is composed of a coating layer, a polymeric material layer and specific antibodies adsorbed on the coating layer; the polymeric material layer is located at the bottom of the chip carrier; the upper surface of the polymeric material layer is attached to the lower surface of the coating layer; the coating layer is provided with several microarrays. Single sperm or extremely small amount of sperms can be stored which realizes the effective storage of single sperm or extremely small amount of sperms, to avoid cross-contamination; in addition, precise access to sperm can be realized, and the maneuverability is very strong, which can solve the requirement of cryopreservation of sperm in patients with very few sperms.

Claims

1. A chip carrier for single sperm or extremely small amount of sperms, comprising a chip carrier (1), an X indicator axis (5), a Y indicator axis (6), and microarrays (3), wherein the chip carrier (1) is composed of a coating layer (2) and a polymeric material layer (4); the polymeric material layer (4) is located at the bottom of the chip carrier (1); the upper surface of the polymeric material layer (4) is attached to the lower surface of the coating layer (2); the coating layer (2) is provided with several microarrays (3).

2. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the adjacent two sides of the coating layer (2) are respectively provided with an X indicator axis (5) and a Y indicator axis (6); the Y indicator axis (6) is provided with capital letters with the same number of columns as the microarray (3) and arranged in order; the polymeric material layer (4) is provided with lowercase letters with the same number of rows as the microarray (3) and arranged in order.

3. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the microarrays (3) are microarrays (3) in the form of specific antibodies.

4. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the coating layer (2) is one or more of polylysine, protamine, and avidin biotin system.

5. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the number of arrays of the microarrays (3) is determined by the area of the polymeric material layer (4) and the coating layer (2); the distance between two adjacent grooves in the microarray (3) is less than 50 nm.

6. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein gray marking lines are provided between two adjacent grooves in the microarray (3).

7. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the size of the chip carrier (1) is less than or equal to 1 mm*1 mm, and a corner of the chip carrier (1) is provided with a pulling wire (7).

8. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the polymeric material in the polymeric material layer (4) is selected from one or several mixtures of polystyrene, nitrocellulose, polyvinyl chloride, and polyethylene.

9. The chip carrier for single sperm or extremely small amount of sperms according to claim 1, wherein the specific antibodies on the microarrays (3) are fixed on the polymeric material layer (4) through the adsorption of the coating layer (2).

10. A storage method for single sperm or extremely small amount of sperms, specifically divided into the following steps: A. processing; placing the sperms to be stored in a glycerol test tube in batches and shaking; B. observing; putting the mixed sperm and glycerin into an observation dish for observation; C. clamping; placing the observation dish under the microscope, and using a dropper to suck a small amount of sperms; D. placing; arranging the sperms orderly on the microarray (3) on the chip carrier (1); E. refrigerating; putting the chip carrier (1) filled with sperms in a liquid nitrogen freezer, and keeping it at −160° C. to −220° C.

Description

BRIEF DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0023] For ease of description, the invention will be described in detail hereinafter by the following specific implementation and drawings.

[0024] FIG. 1 is a schematic diagram of the structure of the invention;

[0025] FIG. 2 is a schematic diagram of the X indicator axis and the Y indicator axis of the invention.

[0026] In the figures, 1 refers to the chip carrier; 2 refers to the coating layer; 3 refers to the microarray; 4 refers to the polymeric material layer; 5 refers to the X indicator axis; 6 refers to the Y indicator axis; 7 refers to the pulling wire.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0027] As shown in FIG. 1-2, the embodiment adopts the following technical solutions: a chip carrier for single sperm or extremely small amount of sperms, comprising a chip carrier 1, an X indicator axis 5, a Y indicator axis 6, and microarrays 3, wherein the chip carrier 1 is composed of a coating layer 2 and a polymeric material layer 4; the polymeric material layer 4 is located at the bottom of the chip carrier 1; the upper surface of the polymeric material layer 4 is attached to the lower surface of the coating layer 2; the coating layer 2 is provided with several microarrays 3.

[0028] The adjacent two sides of the coating layer 2 are respectively provided with an X indicator axis 5 and a Y indicator axis 6; the Y indicator axis 6 is provided with capital letters with the same number of columns as the microarray 3 and arranged in order; the polymeric material layer 4 is provided with lowercase letters with the same number of rows as the microarray 3 and arranged in order; the serial number of each individual sperm is recorded by means of coordinates.

[0029] The microarrays 3 are microarrays 3 in the form of specific antibodies, and the sperm head is fixed on the microarray 3 for precise recognition.

[0030] The coating layer 2 is one or more of polylysine, protamine, and avidin biotin system, which is convenient to fix the specific antibodies of the microarray 3 on the polymeric material, and then use the antibodies to adsorb sperms.

[0031] The number of arrays of the microarrays 3 is determined by the area of the polymeric material layer 4 and the coating layer 2; the distance between two adjacent grooves in the microarray 3 is less than 50 nm, or randomly distributed on the coating layer 2.

[0032] Gray marking lines are provided between two adjacent grooves in the microarray 3 to facilitate the observation and identification of coordinates.

[0033] The size of the chip carrier 1 is less than or equal to 1 mm*1 mm, and a corner of the chip carrier 1 is provided with a pulling wire 7 to facilitate the removal of the chip carrier 1.

[0034] The polymeric material in the polymeric material layer 4 is selected from one or several mixtures of polystyrene, nitrocellulose, polyvinyl chloride, and polyethylene.

[0035] The specific antibodies on the microarrays 3 are fixed on the polymeric material layer 4 through the adsorption of the coating layer 2.

[0036] A storage method for single sperm or extremely small amount of sperms, specifically divided into the following steps:

[0037] A. processing; placing the sperms to be stored in a glycerol test tube in batches and shaking;

[0038] B. observing; putting the mixed sperm and glycerin into an observation dish for observation;

[0039] C. clamping; placing the observation dish under the microscope, and using a dropper to suck a small amount of sperms;

[0040] D. placing; arranging the sperms orderly on the microarray (3) on the chip carrier (1);

[0041] E. refrigerating; putting the chip carrier (1) filled with sperms in a liquid nitrogen freezer, and keeping it at −160° C. to −220° C.

[0042] Specifically: when in use, put the single sperm picked under the microscope on the microarray 3 on the chip carrier 1, and then put the chip into the cryotube for conventional freezing to complete the storage of single sperm or extremely small amount of sperms.

[0043] The adjacent two sides of the coating layer 2 are respectively provided with an X indicator axis 5 and a Y indicator axis 6; the Y indicator axis 6 is provided with capital letters with the same number of columns as the microarray 3 and arranged in order; the polymeric material layer 4 is provided with lowercase letters with the same number of rows as the microarray 3 and arranged in order; the serial number of each individual sperm is recorded by means of coordinates; the microarrays 3 are microarrays 3 in the form of specific antibodies, and the sperm head is fixed on the microarray 3 for precise recognition; the coating layer 2 is one or more of polylysine, protamine, and avidin biotin system, which is convenient to fix the specific antibodies of the microarray 3 on the polymeric material, and then use the antibodies to adsorb sperms; the number of arrays of the microarrays 3 is determined by the area of the polymeric material layer 4 and the coating layer 2; the distance between two adjacent grooves in the microarray 3 is less than 50 nm, or randomly distributed on the coating layer 2; gray marking lines are provided between two adjacent grooves in the microarray 3 to facilitate the observation and identification of coordinates.

[0044] The above shows and describes the basic principles and main features of the invention and the advantages of the invention. Those skilled in the art should understand that the invention is not limited by the above embodiments. The above embodiments and the description in the specification only illustrate the principles of the invention. Various modifications and improvements may be made without departing from the spirit and scope of the invention, which shall all fall within the scope of the claimed invention. The scope of protection claimed by the invention is defined by the appended claims and the equivalents thereof.