METHOD FOR JOINT RECOVERY OF PECTIN FROM CITRUS PEELS AND ALKALINE/ACIDIC PROCESSING WATER FROM CITRUS CANNING

Abstract

Disclosed is a method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, including the following steps: adding fresh citrus fruit peels to alkali/acid processing water from citrus fruit canning for extracting; filtering the resulting mixture, adding to the resulting filtrate 95% ethanol 1 to 3 times the volume of the filtrate, and then adjusting the pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; filtering the product after standing, washing the resulting precipitate with 50% to 70% ethanol, and drying and crushing, thereby obtaining pectin. The method makes full use of waste resources from citrus fruit canning, solves the problem of pollution by processing discharge water, and has the advantages of saving the preparation cost of pectin from citrus peels, and improving the solubility of recovered pectin.

Claims

1.-9. (canceled)

10. A method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning, comprising the following steps: step 1, carrying out extraction: using a method I when involved with alkali processing water from citrus fruit canning, and using a method II when involved with acid processing water from citrus fruit canning, wherein the method I for alkali processing water from citrus fruit canning is as follows: mixing fresh citrus fruit peels with alkali processing water from citrus segment membrane removal and stirring at 10 to 40° C. for 5 to 30 minutes; the method II for acid processing water from citrus fruit canning is as follows: mixing fresh citrus fruit peels with acid processing water from citrus segment membrane removal and stirring at 70 to 95° C. for 60 to 100 minutes; step 2, filtering the mixture from step 1, adding to a resulting filtrate 95 vol % ethanol 1 to 3 times the volume of the filtrate, and adjusting pH to a range of 3.5 to 7, followed by standing for 10 minutes to 4 hours; and step 3, filtering the product from standing in step 2, washing a resulting precipitate with 50 vol % to 70 vol % ethanol once or twice, followed by drying and crushing, thereby obtaining pectin.

11. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 10, wherein: in the method I, a ratio of the fresh citrus fruit peels to the alkali processing water from citrus segment membrane removal is 1 g:(10-15) ml; and in the method II, a ratio of the fresh citrus fruit peels to the acid processing water from citrus segment membrane removal is 1 g:(10-15) ml.

12. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 11, wherein: the method I is as follows: mixing the fresh citrus fruit peels with the alkali processing water from citrus segment membrane removal and stirring after shearing homogenization; and the method II is as follows: mixing the fresh citrus fruit peels with the acid processing water from citrus segment membrane removal and stirring after shearing homogenization.

13. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 11, wherein: the method I is as follows: firstly mixing the fresh citrus fruit peels with the alkali processing water from citrus segment membrane removal in a ratio of 1 g:(1-2) ml, followed by shearing homogenization, then adding the alkali processing water from citrus segment membrane removal until a ratio of the fresh citrus fruit peels to the total alkali processing water from citrus segment membrane removal is 1 g:(10-15) ml, and finally stirring at 10 to 40° C. for 5 minutes to 30 minutes; and the method II is as follows: firstly mixing the fresh citrus fruit peels with the acid processing water from citrus segment membrane removal in a ratio of 1 g:(1-2) ml, followed by shearing homogenization, then adding the acid processing water from citrus segment membrane removal until a ratio of the fresh citrus fruit peels to the total acid processing water from citrus segment membrane removal is 1 g:(10-15) ml, and finally stirring at 70 to 95° C. for 60 minutes to 100 minutes.

14. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 12, wherein the shearing homogenization is: shearing at 200 r/min for 2 minutes.

15. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 13, wherein the shearing homogenization is: shearing at 200 r/min for 2 minutes.

16. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 14, wherein: the filtering in each of the steps 2) and 3) is conducted by a 300 to 400-mesh screen.

17. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 15, wherein: the filtering in each of the steps 2) and 3) is conducted by a 300 to 400-mesh screen.

18. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 16, wherein: in the method I, stirring is carried out at 20 to 30° C. for 10 to 20 minutes; and in the method II, stirring is carried out while heating at 85° C. for 80 to 90 minutes.

19. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 17, wherein: in the method I, stirring is carried out at 20 to 30° C. for 10 to 20 minutes; and in the method II, stirring is carried out while heating at 85° C. for 80 to 90 minutes.

20. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 18, wherein: for alkali processing water from citrus fruit canning, the step 2 comprises: filtering with a 300 to 400-mesh screen, precipitating with 95 vol % ethanol 1 to 2 times the volume of the filtrate, adjusting the pH to a range of 5.5 to 6.5, and standing for 30 to 60 minutes; and for acid processing water from citrus segment membrane removal, the step 2 comprises: filtering with a 350 to 400-mesh screen, precipitating with 95 vol % ethanol 1 to 2 times the volume of the filtrate, adjusting the pH to 3.5, and standing for 30 to 45 minutes.

21. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 19, wherein: for alkali processing water from citrus fruit canning, the step 2 comprises: filtering with a 300 to 400-mesh screen, precipitating with 95 vol % ethanol 1 to 2 times the volume of the filtrate, adjusting the pH to a range of 5.5 to 6.5, and standing for 30 to 60 minutes; and for acid processing water from citrus segment membrane removal, the step 2 comprises: filtering with a 350 to 400-mesh screen, precipitating with 95 vol % ethanol 1 to 2 times the volume of the filtrate, adjusting the pH to 3.5, and standing for 30 to 45 minutes.

22. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 21, wherein: for alkali processing water from citrus fruit canning, the step 3) comprises: filtering with a 350-mesh screen and washing the resulting precipitate with 60 to 70 vol % ethanol; and for acid processing water from citrus segment membrane removal, the step 3) comprises: filtering with a 350-mesh screen and washing the resulting precipitate with 60 to 70 vol % ethanol.

23. The method for joint recovery of pectin from citrus peels and alkaline/acidic processing water from citrus canning according to claim 22, wherein: for alkali processing water from citrus fruit canning, the step 3) comprises: filtering with a 350-mesh screen and washing the resulting precipitate with 60 to 70 vol % ethanol; and for acid processing water from citrus segment membrane removal, the step 3) comprises: filtering with a 350-mesh screen and washing the resulting precipitate with 60 to 70 vol % ethanol.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0057] The present disclosure is now further described in conjunction with specific examples, but the protection scope of the present disclosure is not limited thereto.

I. PROCESS FOR COMBINED RECOVERY OF PECTIN FROM CITRUS FRUIT PEELS AND ALKALI PROCESSING WATER FROM CITRUS FRUIT CANNING (“ALKALI PROCESSING WATER FROM CITRUS FRUIT CANNING” IS EQUAL TO “ALKALI PROCESSING WATER FROM CITRUS SEGMENT MEMBRANE REMOVAL” IN THIS DISCLOSURE)

[0058] In the present disclosure, hydrochloric acid solution at the concentration of 1% was employed in pH adjustment. The alkali processing water from citrus segment membrane removal was alkali processing water from citrus fruit canning.

EXAMPLE 1-1

[0059] Fresh citrus fruit peels from citrus fruit canning were added to the alkali processing water from citrus segment membrane removal in a ratio of 1 g:10 ml and then subjected to shearing at 200 r/min for 2 minutes, stirring at 30° C. for 10 minutes, and filtering with a 350-mesh screen. Then, the filtrate was collected and precipitated with 95% ethanol 2 times the volume of the filtrate (for alcohol precipitation), followed by pH adjustment to 5.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once (a mass ratio of washing solution used to the precipitate was 2:1), dried (at 40° C. for 24 hours), and crushed (sieving through a 60-mesh screen after the crushing), thereby obtaining pectin.

[0060] The pectin obtained by combined recovery had a yield of 6.8% (based on the wet weight of the citrus fruit peels).

[0061] The pectin extract was filtered at a rate of 780 ml/min per square meter of filter cloth, and the solubility of pectin reached 91% after the solution stirring for 15 minutes.

EXAMPLE 1-2

[0062] Fresh citrus fruit peels from citrus fruit canning were added to the alkali processing water from citrus segment membrane removal such that a ratio of the citrus fruit peels to the alkali processing water was firstly 1:1, and subjected to shearing at 200 r/min for 2 minutes. The alkali processing water from citrus segment membrane removal was then added until the ratio of the citrus fruit peels to the processing water was 1:10, followed by stirring at 30° C. for 10 minutes and filtering with a 350-mesh screen. Then, the filtrate was collected and precipitated with 95% ethanol 2 times the volume of the filtrate, followed by pH adjustment to 5.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once, dried and crushed, thereby obtaining pectin.

[0063] The pectin obtained by combined recovery had a yield of 7.2% (based on the wet weight of the citrus fruit peels).

[0064] The pectin extract was filtered at a rate of 860 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

EXAMPLE 1-3

[0065] Fresh citrus fruit peels from citrus fruit canning were added to the alkali processing water from citrus segment membrane removal in a ratio of 1:15 and then subjected to shearing at 200 r/min for 3 minutes, stirring at 20° C. for 20 minutes, and filtering with a 300-mesh screen. Then, the filtrate was collected and precipitated with 95% ethanol 1 time the volume of the filtrate, followed by pH adjustment to 6.5, standing for 60 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0066] The pectin obtained by combined recovery had a yield of 6.3% (based on the wet weight of the citrus fruit peels).

[0067] The pectin extract was filtered at a rate of 840 ml/min per square meter of filter cloth, and the solubility of pectin reached 90% after the solution stirring for 15 minutes.

EXAMPLE 1-4

[0068] Fresh citrus fruit peels from citrus fruit canning were added to the alkali processing water from citrus segment membrane removal such that a ratio of the citrus fruit peels to the alkali processing water was firstly 1:2, and subjected to shearing at 200 r/min for 3 minutes. The alkali processing water from citrus segment membrane removal was then added until the ratio of the citrus fruit peels to the processing water was 1:15, followed by stirring at 20° C. for 20 minutes and filtering with a 300-mesh screen. Then, the filtrate was collected and precipitated with 95% ethanol 1 time the volume of the filtrate, followed by pH adjustment to 6.5, standing for 60 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0069] The pectin obtained by combined recovery had a yield of 6.8% (based on the wet weight of the citrus fruit peels).

[0070] The pectin extract was filtered at a rate of 900 ml/min per square meter of filter cloth, and the solubility of pectin reached 92% after the solution stirring for 15 minutes.

Comparative Example 1

[0071] This example was the same as example 1-1 everywhere except for “shearing at 200 r/min for 2 minutes” which was omitted here, and as a result, the pectin obtained by combined recovery had a yield of 5.8% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 670 ml/min per square meter of filter cloth, and the solubility of pectin reached 89% after the solution stirring for 15 minutes.

Comparative Example 2-1

[0072] According to the existing process of obtaining commercial pectin product, fresh citrus fruit peels were subjected to the steps of citrus fruit peels drying, crushing, extraction, alcohol precipitation, etc. to obtain the commercial pectin product. The process steps and parameters were specifically as follows: the citrus fruit peels were dried at 50° C. for 48 hours, crushed to a size of about 1 mm, added with hydrochloric acid with pH 1.5 and a ratio of the citrus fruit peels to the acid of 1:30 for extraction at 85° C. for 90 minutes, followed by filtering with a 300-mesh screen. The filtrate was collected and precipitated with 1 time 95% ethanol, followed by standing for 60 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0073] As a result, the pectin had a yield of 4.3% (based on the wet weight of the citrus fruit peels).

[0074] The pectin extract was filtered at a rate of 950 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

[0075] In contrast to the technical solutions of the present disclosure, it was necessary in this method to pre-dry the citrus fruit peels before crushing and to prepare a hydrochloric acid solution additionally, leading to greatly increased cost and increased water consumption. Besides, the method failed to recover pectin from ready-made alkali processing water from citrus segment membrane removal.

Comparative Example 2-2

[0076] The alkali processing water from citrus segment membrane removal (i.e., the alkali processing water from citrus fruit canning) was directly subjected to the steps of filtering, alcohol precipitation, drying, crushing, etc. to obtain pectin recovered from alkali processing water. The process steps and parameters were specifically as follows: after filtering with a 350-mesh screen of the alkali processing water from citrus segment membrane removal, the filtrate was collected and added with 2 volumes 95% ethanol, followed by pH adjustment to 5.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once, dried and crushed, thereby obtaining pectin.

[0077] As a result, the alkali processing water from citrus segment membrane removal was filtered at a rate of 630 ml/min per square meter of filter cloth, and the solubility of pectin was 67% after the solution stirring for 15 minutes.

[0078] Thus, compared with example 1-2, a lower filtering rate in single recovery of pectin from the alkali processing water from citrus segment membrane removal and weaker solubility of pectin due to easy aggregation in single recovery could be observed here.

Comparative Example 3-1

[0079] The ratio of the citrus fruit peels to the processing water was changed from “1:10” as used in example 1-2 to “1:5”, and the rest was the same with example 1-2. As a result, the pectin obtained by combined recovery had a yield of 6.7% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 770 ml/min per square meter of filter cloth, and the solubility of pectin reached 92% after the solution stirring for 15 minutes.

Comparative Example 3-2

[0080] The ratio of the citrus fruit peels to the processing water was changed from “1:10” as used in example 1-2 to “1:20”, and the rest was the same with example 1-2. As a result, the pectin obtained by combined recovery had a yield of 7.2% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 910 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

[0081] In spite of a slightly higher filtering rate over example 1-2, the time of filtering was dramatically increased due to greatly increased volume of the extract, and the amount of ethanol used in alcohol precipitation was significantly increased. Therefore, this method would not be recommended.

II. PROCESS FOR COMBINED RECOVERY OF PECTIN FROM CITRUS FRUIT PEELS AND ACID PROCESSING WATER FROM CITRUS FRUIT CANNING (“ACID PROCESSING WATER FROM CITRUS FRUIT CANNING” IS EQUAL TO “ACID PROCESSING WATER FROM CITRUS SEGMENT MEMBRANE REMOVAL” IN THIS DISCLOSURE)

[0082] In the present disclosure, sodium hydroxide solution at the concentration of 1% was employed in pH adjustment. The acid processing water from citrus segment membrane removal was acid processing water from citrus fruit canning.

EXAMPLE 2-1

[0083] Fresh citrus fruit peels from citrus fruit canning were added to the acid processing water from citrus segment membrane removal in a ratio of 1:10 (g/ml) and then subjected to shearing at 200 r/min for 2 minutes, stirring while heating at 85° C. for 90 minutes, and filtering with a 350-mesh screen. Then, the filtrate was collected and added with 95% ethanol 2 times the volume of the filtrate (for alcohol precipitation), followed by pH adjustment to 3.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once (a mass ratio of washing solution used to the precipitate was 2:1), dried (at 40° C. for 24 hours), and crushed (60-mesh sieving after the crushing), thereby obtaining pectin.

[0084] The pectin obtained by combined recovery had a yield of 5.9% (based on the wet weight of the citrus fruit peels).

[0085] The pectin extract was filtered at a rate of 760 ml/min per square meter of filter cloth, and the solubility of pectin reached 91% after the solution stirring for 15 minutes.

EXAMPLE 2-2

[0086] Fresh citrus fruit peels from citrus fruit canning were added to the acid processing water from citrus segment membrane removal such that a ratio of the citrus fruit peels to the alkali processing water was firstly 1:1, and subjected to shearing at 200 r/min for 2 minutes. The acid processing water from citrus segment membrane removal was then added until the ratio of the citrus fruit peels to the processing water was 1:10, followed by stirring while heating at 85° C. for 90 minutes and filtering with a 350-mesh screen. Then, the filtrate was collected and added with 95% ethanol 2 times the volume of the filtrate, followed by pH adjustment to 3.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once, dried and crushed, thereby obtaining pectin.

[0087] The pectin obtained by combined recovery had a yield of 6.2% (based on the wet weight of the citrus fruit peels).

[0088] The pectin extract was filtered at a rate of 830 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

EXAMPLE 2-3

[0089] Fresh citrus fruit peels from citrus fruit canning were added to the acid processing water from citrus segment membrane removal such that a ratio of the citrus fruit peels to the alkali processing water was firstly 1:1, and subjected to shearing at 200 r/min for 2 minutes. The acid processing water from citrus segment membrane removal was then added until the ratio of the citrus fruit peels to the processing water was 1:10, followed by stirring while heating at 85° C. for 80 minutes and filtering with a 400-mesh screen. Then, the filtrate was collected and added with 95% ethanol 1 time the volume of the filtrate, followed by pH adjustment to 3.5, standing for 45 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0090] The pectin obtained by combined recovery had a yield of 6.1% (based on the wet weight of the citrus fruit peels).

[0091] The pectin extract was filtered at a rate of 800 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

EXAMPLE 2-4

[0092] Fresh citrus fruit peels from citrus fruit canning were added to the acid processing water from citrus segment membrane removal such that a ratio of the citrus fruit peels to the alkali processing water was firstly 1:2, and subjected to shearing at 200 r/min for 2 minutes. The acid processing water from citrus segment membrane removal was then added until the ratio of the citrus fruit peels to the processing water was 1:15, followed by stirring while heating at 85° C. for 80 minutes and filtering with a 400-mesh screen. Then, the filtrate was collected and added with 95% ethanol 1 time the volume of the filtrate, followed by pH adjustment to 3.5, standing for 45 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0093] The pectin obtained by combined recovery had a yield of 6.2% (based on the wet weight of the citrus fruit peels).

[0094] The pectin extract was filtered at a rate of 840 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

Comparative Example 4

[0095] This example was the same as example 2-1 everywhere except for “shearing at 200 r/min for 2 minutes” which was omitted here, and as a result, the pectin obtained by combined recovery had a yield of 4.9% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 640 ml/min per square meter of filter cloth, and the solubility of pectin reached 89% after the solution stirring for 15 minutes.

Comparative Example 5-1

[0096] According to the existing process of obtaining commercial pectin product, fresh citrus fruit peels were subjected to the steps of citrus fruit peels drying, crushing, extraction, alcohol precipitation, etc. to obtain the commercial pectin product. The process steps and parameters were specifically as follows: the citrus fruit peels were dried at 50° C. for 48 hours, crushed to a size of about 1 mm, added with hydrochloric acid with pH 1.5 and a ratio of the citrus fruit peels to the acid of 1:30 for extraction at 85° C. for 90 minutes, followed by filtering with a 300-mesh screen. The filtrate was collected and added with 1 time 95% ethanol, followed by standing for 60 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 60% ethanol once, dried and crushed, thereby obtaining pectin.

[0097] As a result, the pectin had a yield of 4.3% (based on the wet weight of the citrus fruit peels).

[0098] The pectin extract was filtered at a rate of 950 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

[0099] In contrast to the technical solutions of the present disclosure, it was necessary in this method to pre-dry the citrus fruit peels before crushing and to prepare a hydrochloric acid solution additionally, leading to greatly increased cost and increased water consumption. Besides, the method failed to recover pectin from ready-made acid processing water from citrus segment membrane removal.

Comparative Example 5-2

[0100] The acid processing water from citrus segment membrane removal (i.e., the acid processing water from citrus fruit canning) was directly subjected to the steps of filtering, alcohol precipitation, drying, crushing, etc. to obtain pectin recovered from acid processing water. The process steps and parameters were specifically as follows: after filtering with a 350-mesh screen of the acid processing water from citrus segment membrane removal, the filtrate was collected and added with 2 times 95% ethanol, followed by pH adjustment to 3.5, standing for 30 minutes, precipitation, and separation of precipitate by filtering with a 350-mesh screen. The precipitate was washed with 70% ethanol once, dried and crushed, thereby obtaining pectin.

[0101] As a result, the acid processing water from citrus segment membrane removal was filtered at a rate of 590 ml/min per square meter of filter cloth, and the solubility of pectin was 65% after the solution stirring for 15 minutes.

[0102] Thus, compared with example 2-2, a lower filtering rate in single recovery of pectin from the acid processing water from citrus segment membrane removal and weaker solubility of pectin due to easy aggregation in single recovery could be observed here.

Comparative Example 6-1

[0103] The ratio of the citrus fruit peels to the processing water was changed from “1:10” as used in example 2-2 to “1:5”, and the rest was the same with example 2-2. As a result, the pectin obtained by combined recovery had a yield of 5.9% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 740 ml/min per square meter of filter cloth, and the solubility of pectin reached 92% after the solution stirring for 15 minutes.

Comparative Example 6-2

[0104] The ratio of the citrus fruit peels to the processing water was changed from “1:10” as used in example 2-2 to “1:20”, and the rest was the same with example 2-2. As a result, the pectin obtained by combined recovery had a yield of 6.2% (based on the wet weight of the citrus fruit peels). The pectin extract was filtered at a rate of 870 ml/min per square meter of filter cloth, and the solubility of pectin reached 93% after the solution stirring for 15 minutes.

[0105] In spite of a slightly higher filtering rate over example 2-2, the time of filtering was dramatically increased due to greatly increased volume of the extract, and the amount of ethanol used in alcohol precipitation was significantly increased. Therefore, this method would not be recommended.

[0106] Finally, it should be noted that the examples listed above are merely a few specific examples of the present disclosure. Apparently, the present disclosure would not be limited to the above examples, and many variations are possible. All modifications that can be directly derived or conceived by a person of ordinary skill in the art from the specification of the present disclosure should be regarded as falling into the protection scope of the present disclosure.