Mono- or di-substituted indole derivatives as dengue viral replication inhibitors

Abstract

The present invention concerns mono- or di-substituted indole derivatives (I) which are useful to prevent or treat dengue viral infections and also relates to said compounds for use as a medicine, more preferably for use as a medicine to treat or prevent dengue viral infections. The present invention furthermore relates to pharmaceutical compositions or combination preparations of the compounds, to the compositions or preparations for use as a medicine, more preferably for the prevention or treatment of dengue viral infections. The invention also relates to processes for preparation of the compounds.

##STR00001##

Claims

1. A compound of formula (I) ##STR00022## wherein: when R.sub.1 is H, and R.sub.2 is F then R.sub.3 is H, F or CH.sub.3; when R.sub.1 is F or CH.sub.3, R.sub.2 is OCH.sub.3 then R.sub.3 is H; when R.sub.1 is F, and R.sub.2 is H then R.sub.3 is CH.sub.3; when R.sub.1 is H, and R.sub.2 is OCH.sub.3 then R.sub.3 is H; when R.sub.1 is H, and R.sub.2 is Cl then R.sub.3 is H or CH.sub.3; when R.sub.1 is F, and R.sub.2 is F then R.sub.3 is H; and when R.sub.1 is CH.sub.3, and R.sub.2 is H then R.sub.3 is F; or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof.

2. A compound selected from the group consisting of: ##STR00023## ##STR00024## ##STR00025##

3. A pharmaceutical composition comprising a compound of formula (I) or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof; according to claim 1 and one or more pharmaceutically acceptable excipients, diluents or carriers.

4. (canceled)

5. A method of treating a dengue viral infection, comprising administering a therapeutically effective amount of at least one compound or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof as claimed in claim 1.

6. A method of inhibiting the replication of dengue virus in a biological sample or patient, comprising administering a therapeutically effective amount of at least one compound or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof as claimed in claim 1. ##STR00026##

7. The method as claimed in claim 6 further comprising co-administering an additional therapeutic agent.

8. The method as claimed in claim 7 wherein said additional therapeutic agent is at least one selected from the group consisting of an antiviral agent and a dengue vaccine.

9. A pharmaceutical composition comprising a compound or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof according to claim 2 and one or more pharmaceutically acceptable excipients, diluents or carriers.

10. A method of treating a dengue viral infection, comprising administering a therapeutically effective amount of at least one compound or a stereo-isomeric form, a pharmaceutically acceptable salt, solvate or polymorph thereof as claimed in claim 2.

Description

EXAMPLES

[0040] LC/MS Methods

[0041] The High Performance Liquid Chromatography (HPLC) measurement was performed using a LC pump, a diode-array (DAD) or a UV detector and a column as specified in the respective methods. If necessary, additional detectors were included (see table of methods below).

[0042] Flow from the column was brought to the Mass Spectrometer (MS) which was configured with an atmospheric pressure ion source. It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time . . . ) in order to obtain ions allowing the identification of the compound's nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software.

[0043] Compounds are described by their experimental retention times (R.sub.t) and ions. If not specified differently in the table of data, the reported molecular ion corresponds to the [M+H].sup.+ (protonated molecule) and/or [M−H].sup.− (deprotonated molecule). In case the compound was not directly ionizable the type of adduct is specified (i.e. [M+NH.sub.4].sup.+, [M+HCOO].sup.−, etc. . . . ). For molecules with multiple isotopic patterns (Br, Cl), the reported value is the one obtained for the lowest isotope mass. All results were obtained with experimental uncertainties that are commonly associated with the method used.

[0044] Hereinafter, “SQD” means Single Quadrupole Detector, “MSD” Mass Selective Detector, “RT” room temperature, “BEH” bridged ethylsiloxane/silica hybrid, “DAD” Diode Array Detector, “HSS” High Strength silica.

[0045] LC/MS Method codes (Flow expressed in mL/min; column temperature (T) in ° C.; Run time in minutes)

TABLE-US-00001 Run Method Flow time code Instrument Column Mobile phase Gradient Col T (min) LC-A Waters: Waters: BEH A: 10 mM CH.sub.3COONH.sub.4 in From 95% A to 5% A 0.8 mL/min 2 Acquity ® C18 (1.7 μm, 95% H.sub.2O + 5% CH.sub.3CN in 1.3 min, held 55° C. UPLC ® - 2.1 × 50 mm) B: CH.sub.3CN for 0.7 min. DAD-SQD LC-B Waters: Waters: HSS A: 10 mM CH.sub.3COONH.sub.4 in From 100% A to 5% A 0.7 mL/min 3.5 Acquity ® T3 (1.8 μm, 95% H.sub.2O + 5% CH.sub.3CN in 2.10 min, 55° C. UPLC ® - 2.1 × 100 mm) B: CH.sub.3CN to 0% A in 0.90 min, DAD-SQD to 5% A in 0.5 min LC-C Waters: Waters: BEH A: 95% CH.sub.3COONH.sub.4 84.2% A for 0.49 min, 0.343 mL/min 6.2 Acquity ® C18 (1.7 μm, 7 mM/5% CH.sub.3CN, to 10.5% A in 2.18 min, 40° C. UPLC ® - 2.1 × 100 mm) B: CH.sub.3CN held for 1.94 min, back DAD-Quattro to 84.2% A in 0.73 min, Micro ™ held for 0.73 min. LC-D Waters: Waters: HSS A: 0.1% Formic 50% A to 10% in 0.5 mL/min 5 Acquity ® C18 (1.8 μm, acid in H.sub.2O 3.5 min, held for 40° C. UPLC ® - 2.1 × 50 mm B: CH.sub.3CN 1.5 min. DAD-TQD

[0046] SFC-MS Methods

[0047] The SFC measurement was performed using an Analytical Supercritical fluid chromatography (SFC) system composed by a binary pump for delivering carbon dioxide (CO2) and modifier, an autosampler, a column oven, a diode array detector equipped with a high-pressure flow cell standing up to 400 bars.

[0048] If configured with a Mass Spectrometer (MS) the flow from the column was brought to the (MS). It is within the knowledge of the skilled person to set the tune parameters (e.g. scanning range, dwell time . . . ) in order to obtain ions allowing the identification of the compound's nominal monoisotopic molecular weight (MW). Data acquisition was performed with appropriate software.

[0049] Analytical SFC-MS Methods (Flow expressed in mL/min; column temperature (T) in ° C.; Run time in minutes, Backpressure (BPR) in bars.

TABLE-US-00002 Method Flow Run time code column mobile phase gradient Col T BPR SFC-A Daicel Chiralpak ® AD- A: CO.sub.2 30% B hold 7 min, 3 7 H column (5 μm, 150 × B: MeOH 35 100 4.6 mm) SFC-B Daicel Chiralpak ® AD- A: CO.sub.2 40% B hold 7 min, 3 7 H column (5 μm, 150 × B: MeOH 35 100 4.6 mm) SFC-C Daicel Chiralcel ® OJ-H A: CO.sub.2 40% B hold 7 min, 3 7 column (5 μm, 250 × B: MeOH 35 100 4.6 mm) SFC-D Daicel Chiralcel ® OD- A: CO.sub.2 40% B hold 7 min, 3 7 H column (5 μm, 150 × B: MeOH 35 100 4.6 mm) SFC-E WHELK-O1 (S,S) A: CO.sub.2 60% B hold 7 min, 3 7 250 * 4.6 mm 5 μm Regis B: MeOH 35 100 SFC-F Daicel Chiralpak ® AS3 A: CO.sub.2 25% B hold 6 min, 2.5 9.5 column (3.0 μm, 150 × B: EtOH + to 50% in 40 110 4.6 mm) 0.2% iPrNH.sub.2 + 1 min hold 3% H.sub.2O 2.5 min SFC-G Daicel Chiralpak ® AS3 A: CO.sub.2 30% B hold 6 min, 2.5 9.5 column (3.0 μm, 150 × B: EtOH + to 50% in 40 110 4.6 mm) 0.2% iPrNH.sub.2 + 1 min hold 3% H.sub.2O 2.5 min

[0050] Melting Points

[0051] Values are either peak values or melt ranges, and are obtained with experimental uncertainties that are commonly associated with this analytical method.

[0052] DSC823e (Indicated as DSC)

[0053] For a number of compounds, melting points were determined with a DSC823e (Mettler-Toledo). Melting points were measured with a temperature gradient of 10° C./minute. Maximum temperature was 300° C.

[0054] Optical Rotations:

[0055] Optical rotations were measured on a Perkin-Elmer 341 polarimeter with a sodium lamp and reported as follows: [α].sup.o (λ, c g/100 ml, solvent, T° C.).

[0056] [α].sub.λ.sup.T=(100α)/(I×c): where I is the path length in dm and c is the concentration in g/100 ml for a sample at a temperature T (° C.) and a wavelength λ (in nm). If the wavelength of light used is 589 nm (the sodium D line), then the symbol D might be used instead. The sign of the rotation (+ or −) should always be given. When using this equation the concentration and solvent are always provided in parentheses after the rotation. The rotation is reported using degrees and no units of concentration are given (it is assumed to be g/100 ml).

Example 1: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 1) and Chiral Separation into Enantiomers 1A and 1B

[0057] ##STR00009##

[0058] Synthesis of Intermediate 1a:

[0059] 2-(4-Chloro-2-methoxyphenyl)acetic acid [CAS 170737-95-8] (5.8 g, 28.9 mmol) was added in small portions to thionyl chloride (50 mL) and the resulting solution was stirred overnight at 60° C. The solvent was concentrated under reduced pressure and co-evaporated with toluene to give 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (6.5 g) as an oily residue that was used without further purification in the next step.

[0060] Synthesis of Intermediate 1b:

[0061] Diethylaluminum chloride 1M in hexane (37.1 mL, 37.14 mmol) was added dropwise at 0° C. to a solution of 6-fluoro-1H-indole [CAS 399-51-9] (3.34 g, 24.76 mmol) in CH.sub.2Cl.sub.2 (100 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (6.3 g, 28.76 mmol) in CH.sub.2Cl.sub.2 (100 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. Ice-water was added and the precipitate was filtered off, washed with water and a small amount of CH.sub.2Cl.sub.2. The solids were dried under vacuum at 70° C. overnight to give 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)ethanone 1b (4.9 g).

[0062] Synthesis of Intermediate 1c:

[0063] At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (5.8 g, 15.4 mmol) in THF (65 mL) was added dropwise to a mixture of 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)ethanone 1b (4.9 g, 15.4 mmol) in THF (60 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with EtOAc and washed with water. A precipitate appeared in the organic layer and was filtered off and dried to provide a first batch of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)ethanone 1c (4.6 g). The organic layer was separated, dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The residue was crystallized from EtOAc, the precipitate was filtered off, washed with Et.sub.2O and dried under vacuum to provide a second fraction of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)ethanone 1c (1.6 g).

[0064] Synthesis of Compound 1 and Chiral Separation into Enantiomers 1A and 1B:

[0065] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)-ethanone 1c (2.1 g, 5.3 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (924 mg, 5.05 mmol) and triethylamine (1.47 mL, 10.6 mmol) in CH.sub.3CN (16 mL) in a sealed tube was heated at 100° C. for 30 min using a microwave Biotage® Initiator EXP 60 with a power output ranging from 0 to 400 W (fixed hold time). The reaction was diluted with CH.sub.2Cl.sub.2 and the organic layer was washed with water, dried over MgSO.sub.4, filtered and the solvent was concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel (15-40 μm, 80 g) using a heptane/EtOAc gradient of 50/50 to 0/100. The pure fractions were collected and concentrated to give 1.1 g of Compound 1. This fraction was combined with another batch of 0.93 g of Compound 1 and subsequently purified via achiral SFC (Stationary phase: CYANO 6 μm 150×21.2 mm, Mobile phase: 75% CO.sub.2, 25% MeOH) to provide 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 1, 1.36 g) as a racemic mixture.

[0066] The enantiomers of Compound 1 (1.36 g) were separated via Chiral SFC (Stationary phase: Chiracel® OJ 20×250 mm, Mobile phase: 60% CO.sub.2, 40% MeOH) yielding 611 mg of the first eluted enantiomer and 586 mg of the second eluted enantiomer. The first eluted enantiomer was taken up with CH.sub.3CN/diisopropylether/heptane. The precipitate was filtered off and dried to give Enantiomer 1A (496 mg) as an amorphous powder. The second eluted enantiomer was taken up with CH.sub.3CN/diisopropylether/heptane. The precipitate was filtered off and dried to give Enantiomer 1B (458 mg) as an amorphous powder.

[0067] Compound 1:

[0068] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.3 Hz, 2H) 3.77-3.88 (m, 2H) 3.96 (s, 3H) 4.78 (t, J=5.5 Hz, 1H) 5.71 (t, J=1.9 Hz, 1H) 5.93 (d, J=1.9 Hz, 2H) 6.15 (d, J=8.2 Hz, 1H) 6.40 (d, J=8.2 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 7.02-7.08 (m, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.27 (dd, J=9.6, 2.4 Hz, 1H) 7.35 (d, J=8.5 Hz, 1H) 8.13 (dd, J=8.8, 5.7 Hz, 1H) 8.43 (s, 1H) 11.96-12.17 (m, 1H)

[0069] LC/MS (method LC-C): R.sub.t 2.95 min, MH.sup.+ 499

[0070] Enantiomer 1A:

[0071] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.57-3.68 (m, 5H) 3.77-3.89 (m, 2H) 3.96 (s, 3H) 4.73-4.87 (m, 1H) 5.71 (t, J=1.9 Hz, 1H) 5.91-5.96 (m, 2H) 6.15 (d, J=8.2 Hz, 1H) 6.39 (d, J=8.2 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 7.01-7.11 (m, 2H) 7.27 (dd, J=9.6, 2.4 Hz, 1H) 7.36 (d, J=8.2 Hz, 1H) 8.13 (dd, J=9.6, 5.7 Hz, 1H) 8.43 (s, 1H) 11.45-12.31 (m, 1H)

[0072] LC/MS (method LC-C): R.sub.t 2.95, MH.sup.+ 499

[0073] [α].sub.D.sup.20: +112.1° (c 0.281, DMF)

[0074] Chiral SFC (method SFC-C): R.sub.t 3.17 min, MH.sup.+ 499, chiral purity 100%.

[0075] Enantiomer 1B:

[0076] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.57-3.67 (m, 5H) 3.74-3.90 (m, 2H) 3.96 (s, 3H) 4.78 (br. s., 1H) 5.70-5.74 (m, 1H) 5.93 (s, 2H) 6.15 (d, J=8.2 Hz, 1H) 6.40 (d, J=8.2 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 7.02-7.08 (m, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.27 (dd, J=9.6, 2.4 Hz, 1H) 7.36 (d, J=8.2 Hz, 1H) 8.13 (dd, J=9.6, 5.5 Hz, 1H) 8.43 (s, 1H) 11.63-12.47 (m, 1H)

[0077] LC/MS (method LC-C): R.sub.t 2.95, MH.sup.+ 499

[0078] [α].sub.D.sup.20: −113.9° (c 0.28, DMF)

[0079] Chiral SFC (method SFC-C): R.sub.t 4.12 min, MH.sup.+ 499, chiral purity 100%.

Example 1.1: Chiral Stability of Enantiomer 1A at pH 7.4

[0080] The chiral stability of Enantiomer 1A (R=OMe) was evaluated by determination of the enantiomeric excess (ee %) after incubation for 24 h and 48 h in a buffered solution at pH 7.4 at 40° C. and 60° C. To assess the influence of the methoxy-substituent of Enantiomer 1A (R=OMe) on the stability against racemization, the chiral stability of Enantiomer 1′A (R═H) was tested under the same conditions.

[0081] To this end, 5 μM buffered (pH=7.4) solutions of 1A and 1′A were prepared by mixing 25 μL of a 100 μM solution of 1A or 1′A in DMSO with 475 μL aqueous buffer pH 7.4. Samples were taken 24 h and 48 h after incubation at 40° C. and 60° C. The analytical samples were analyzed by Chiral SFC (MS detection) and the chiral purity was expressed as the enantiomeric excess (ee %=% enantiomer A−% enantiomer B). Both Enantiomers 1A and 1′A had a chiral purity of 100% prior to their incubation.

##STR00010##

TABLE-US-00003 ee % Sampling timepoints (h) Compound Temperature 24 48 1A.sup.  40° C. 100 100 60° C. 99 96 1′A 40° C. 69 41 60° C. 0 0

Example 2: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 2) and Chiral Separation into Enantiomers 2A and 2B

[0082] ##STR00011##

[0083] Synthesis of Intermediate 2a:

[0084] A solution of 6-fluoro-7-methyl-1H-indole [CAS 57817-10-4] (1.10 g, 7.37 mmol) in CH.sub.2Cl.sub.2 (40 mL) was cooled on an ice-bath under N.sub.2-flow. Diethylaluminum chloride 1M in hexane (10 mL, 10 mmol) was added dropwise over 15 min. After additional stirring for 15 min at 0° C., a solution of 2-(4-chloro-2-methoxy-phenyl)acetyl chloride 1a (2.06 g, 9.42 mmol, synthesis: see example 1) in CH.sub.2Cl.sub.2 (35 mL) was added over 75 min at 0° C. The reaction was stirred at 0° C. for 1 h and subsequently quenched by slow addition of a solution of potassium sodium tartrate tetrahydrate (Rochelle salt) [CAS 6100-16-9] (4.24 g, 15 mmol) in water (10 mL), while keeping the internal temperature of the mixture below 10° C. The ice-bath was removed, 2-methyl-THF (160 mL) and Na.sub.2SO.sub.4 (60 g) were added and the resulting mixture was stirred at room temperature overnight. The mixture was filtered over Dicalite® and the filter cake was washed with several portions of THF. The combined filtrates were evaporated under reduced pressure and the residue was triturated with a small amount of CH.sub.2Cl.sub.2. The solids were isolated by filtration and dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)ethanone 2a (1.9 g) as a white powder.

[0085] Synthesis of Compound 2 and Chiral Separation into Enantiomers 2A and 2B:

[0086] A solution of 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)-ethanone 2a (1.9 g, 5.73 mmol) in dry THF (60 mL) was cooled on an ice-bath under N.sub.2-flow. At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.3 g, 5.91 mmol) in THF (50 mL) was added dropwise over a period of 1 h and the mixture was stirred at 0° C. for an additional 1.5 h. The reaction mixture was concentrated under reduced pressure and the residue, containing the crude brominated intermediate 2b, was dissolved in CH.sub.3CN (100 mL). 2-(3-Amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (2.11 g, 11.5 mmol) and diisopropylethylamine (2 mL, 11.6 mmol) were added and the reaction mixture was stirred at room temperature for 3 days. Water (350 mL) was added and the reaction products were extracted with 2-methyl-THF (3×100 mL). The combined organic layers were washed with 0.5 M HCl (200 mL) and water (3×300 mL), dried over MgSO.sub.4 and evaporated under reduced pressure. The residue (2.48 g) was purified by column chromatography (Stationary phase: Silica 40 g, HP-Spher® 40 μm; Mobile phase: heptane/EtOAc gradient 100/0 to 0/100). The fractions containing reaction product were combined and evaporated under reduced pressure. The residue was triturated with a small amount of a mixture of EtOAc/heptane (1/1), the solids were filtered off and dried under vacuum to provide 2-(4-chloro-2-methoxyphenyl)-1-(6-fluoro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 2, 1.38 g) as a racemic mixture. The enantiomers of Compound 2 (1.38 g) were separated via Preparative SFC (Stationary phase: Chiralpak® Diacel AS 20×250 mm, Mobile phase: CO.sub.2, EtOH with 0.4% iPrNH.sub.2). The first eluted enantiomer was dissolved in a mixture of MeOH (50 mL) and water (20 mL) and the mixture was evaporated under reduced pressure (200 mbar, water bath 40° C.) to a residual volume of 20 ml. The resulting suspension was diluted with 20 ml water and stirred at room temperature for 3 h. A white solid was filtered off, washed with water and dried under vacuum at room temperature to give Enantiomer 2A (631 mg) as an amorphous white powder. The second eluted enantiomer was dissolved in a mixture of MeOH (50 mL) and water (20 mL) and the mixture was evaporated under reduced pressure (200 mbar, water bath 40° C.) to a residual volume of 20 ml. The resulting suspension was diluted with 20 ml water and stirred at room temperature for 3 h. A white solid was filtered off, washed with water and dried under vacuum at room temperature to give Enantiomer 2B (625 mg) as an amorphous white powder.

[0087] Compound 2:

[0088] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.37 (br s, 3H) 3.60 (s, 3H) 3.63 (q, J=5.2 Hz, 2H) 3.76-3.89 (m, 2H) 3.96 (s, 3H) 4.76 (t, J=5.5 Hz, 1H) 5.71 (t, J=2.1 Hz, 1H) 5.94 (d, J=2.2 Hz, 2H) 6.16 (d, J=8.1 Hz, 1H) 6.36 (d, J=8.1 Hz, 1H) 6.95 (dd, J=8.4, 2.0 Hz, 1H) 7.00 (dd, J=10.1, 8.8 Hz, 1H) 7.08 (d, J=2.0 Hz, 1H) 7.35 (d, J=8.1 Hz, 1H) 7.95 (dd, J=8.7, 5.2 Hz, 1H) 8.41 (s, 1H) 12.17 (br s, 1H)

[0089] LC/MS (method LC-A): R.sub.t 1.19 min, MH.sup.+ 513

[0090] Enantiomer 2A:

[0091] .sup.1H NMR (360 MHz, DMSO-d.sub.6) δ ppm 2.38 (s, 3H) 3.61 (s, 3H) 3.62-3.67 (m, 2H) 3.82 (ddt, J=15.4, 10.2, 5.1, 5.1 Hz, 2H) 3.97 (s, 3H) 4.81 (t, J=5.5 Hz, 1H) 5.71 (br t, J=1.8 Hz, 1H) 5.95 (d, J=1.5 Hz, 2H) 6.17 (br d, J=8.4 Hz, 1H) 6.40 (br d, J=8.1 Hz, 1H) 6.96 (dd, J=8.4, 1.8 Hz, 1H) 7.02 (br dd, J=10.1, 9.0 Hz, 1H) 7.10 (d, J=1.8 Hz, 1H) 7.36 (d, J=8.1 Hz, 1H) 7.96 (dd, J=8.6, 5.3 Hz, 1H) 8.44 (s, 1H) 12.22 (br s, 1H)

[0092] LC/MS (method LC-A): R.sub.t 1.20, MH.sup.+ 513

[0093] [α].sub.D.sup.20 : +83.3° (c 0.36, DMF)

[0094] Chiral SFC (method SFC-F): R.sub.t 2.05 min, MH.sup.+ 513, chiral purity 100%

[0095] Enantiomer 2B:

[0096] .sup.1H NMR (360 MHz, DMSO-d.sub.6) δ ppm 2.38 (s, 3H) 3.61 (s, 3H) 3.62-3.67 (m, 2H) 3.83 (qt, J=10.2, 5.1 Hz, 2H) 3.97 (s, 3H) 4.80 (t, J=5.5 Hz, 1H) 5.71 (br t, J=2.2 Hz, 1H) 5.95 (d, J=1.8 Hz, 2H) 6.17 (d, J=8.1 Hz, 1H) 6.40 (d, J=8.1 Hz, 1H) 6.96 (dd, J=8.4, 1.8 Hz, 1H) 7.02 (dd, J=10.2, 8.8 Hz, 1H) 7.10 (d, J=1.8 Hz, 1H) 7.36 (d, J=8.1 Hz, 1H) 7.96 (dd, J=8.6, 5.3 Hz, 1H) 8.44 (s, 1H) 12.21 (br s, 1H)

[0097] LC/MS (method LC-A): R.sub.t 1.20, MH.sup.+ 513

[0098] [α].sub.D.sup.20: −81.9° (c 0.515, DMF)

[0099] Chiral SFC (method SFC-F): R.sub.t 3.28 min, MH.sup.+ 513, chiral purity 100%

Example 3: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 3) and Chiral Separation into Enantiomers 3A and 3B

[0100] ##STR00012##

[0101] Synthesis of Intermediate 3a:

[0102] Diethylaluminum chloride 1M in hexane (18.1 mL, 18.1 mmol) was added dropwise at 0° C. to a solution of 6-chloro-7-methyl-1H-indole [CAS 57817-09-1] (2 g, 12.08 mmol) in CH.sub.2Cl.sub.2 (60 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.21 g, 14.66 mmol, synthesis: see example 1) in CH.sub.2Cl.sub.2 (60 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. Ice-water was added and the precipitate was filtered off and washed with water. The solid was dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)ethanone 3a (3.2 g).

[0103] Synthesis of Intermediate 3b:

[0104] At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.63 g, 9.65 mmol) in THF (85 mL) was added dropwise to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)ethanone 3a (3.2 g, 9.2 mmol) in THF (85 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with a minimum of CH.sub.3CN/diisopropylether. The precipitate was filtered off and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)ethanone 3b (4.1 g).

[0105] Synthesis of Compound 3 and Chiral Separation into Enantiomers 3A and 3B:

[0106] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)ethanone 3b (3.1 g, 7.26 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (1.33 g, 7.26 mmol) and diisopropylethylamine (1.9 mL, 10.9 mmol) in CH.sub.3CN/THF (1/1) (120 mL) was stirred at 70° C. for 24 h. The mixture was concentrated under reduced pressure. The residue was diluted with CH.sub.2Cl.sub.2 and washed with 1N HCl. The organic layer was separated, dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel (15-40 μm, 80 g in CH.sub.2Cl.sub.2/MeOH (99.5/0.5)). The pure fractions were collected and evaporated under reduced pressure (2.3 g). A small amount was crystallized from Et.sub.2O/CH.sub.3CN to provide an analytical sample of 2-(4-chloro-2-methoxyphenyl)-1-(6-chloro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 3) as a racemate.

[0107] The Enantiomers of Compound 3 (2.2 g) were separated via Preparative Chiral SFC (Stationary phase: (S,S) Whelk-O 1 5 μm 250×21.1 mm, Mobile phase: 45% CO.sub.2, 55% EtOH (+2% CH.sub.2Cl.sub.2)) to give 1.11 g of the first eluted enantiomer and 1.07 g of the second eluted enantiomer. The first eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O/heptane to afford Enantiomer 3A (461 mg) as an amorphous white powder. The second eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O/heptane to afford Enantiomer 3B (872 mg) as an amorphous white powder.

[0108] Compound 3:

[0109] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 12.26 (d, J=2.8 Hz, 1H) 8.46 (d, J=3.2 Hz, 1H) 7.97 (d, J=8.5 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.22 (d, J=8.5 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 6.40 (d, J=8.2 Hz, 1H) 6.18 (d, J=8.2 Hz, 1H) 5.95 (d, J=2.2 Hz, 2H) 5.71 (t, J=2.2 Hz, 1H) 4.79 (t, J=5.5 Hz, 1H) 3.96 (s, 3H) 3.77-3.89 (m, 2H) 3.58-3.67 (m, 5H)

[0110] LC/MS (method LC-C): R.sub.t 3.28 min, MH.sup.+ 529

[0111] Melting point: 220° C.

[0112] Enantiomer 3A:

[0113] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 12.26 (br. s., 1H) 8.46 (s, 1H) 7.97 (d, J=8.2 Hz, 1H) 7.36 (d, J=8.2 Hz, 1H) 7.21 (d, J=8.5 Hz, 1H) 7.10 (d, J=1.9 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 6.40 (d, J=7.9 Hz, 1H) 6.18 (d, J=8.2 Hz, 1H) 5.95 (d, J=2.2 Hz, 2H) 5.71 (t, J=2.0 Hz, 1H) 4.79 (t, J=5.5 Hz, 1H) 3.97 (s, 3H) 3.77-3.89 (m, 2H) 3.59-3.67 (m, 5H)

[0114] LC/MS (method LC-C): R.sub.t 3.27 min, MH.sup.+ 529

[0115] [α].sub.D.sup.20: +88.8° (c 0.2691, DMF)

[0116] Chiral SFC (method SFC-E): R.sub.t 3.40 min, MH.sup.+ 529, chiral purity 100%.

[0117] Enantiomer 3B:

[0118] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 12.26 (br. s., 1H) 8.45 (s, 1H) 7.97 (d, J=8.5 Hz, 1H) 7.36 (d, J=8.5 Hz, 1H) 7.21 (d, J=8.5 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 6.40 (d, J=8.2 Hz, 1H) 6.18 (d, J=8.2 Hz, 1H) 5.95 (d, J=2.2 Hz, 2H) 5.71 (t, J=2.0 Hz, 1H) 4.79 (t, J=5.5 Hz, 1H) 3.97 (s, 3H) 3.76-3.90 (m, 2H) 3.60-3.66 (m, 5H)

[0119] LC/MS (method LC-C): R.sub.t 3.27 min, MH.sup.+ 529

[0120] [α].sub.D.sup.20: −87.4° (c 0.2564, DMF)

[0121] Chiral SFC (method SFC-E): R.sub.t 4.19 min, MH.sup.+ 529, chiral purity 100%.

Example 4: Synthesis of 1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 4) and Chiral Separation into Enantiomers 4A and 4B

[0122] ##STR00013##

[0123] Synthesis of Intermediate 4a:

[0124] Diethylaluminum chloride 1M in hexane (19.8 mL, 19.8 mmol) was added dropwise at 0° C. to a solution of 6-chloro-1H-indole [CAS 17422-33-2] (2 g, 13.2 mmol) in CH.sub.2Cl.sub.2 (45 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.36 g, 15.3 mmol, synthesis: see example 1) in CH.sub.2Cl.sub.2 (45 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. Ice-water was added and the precipitate was filtered off, washed with water and the minimum of CH.sub.2Cl.sub.2. The solid was dried under vacuum to give 1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)ethanone 4a (2.68 g).

[0125] Synthesis of Intermediate 4b:

[0126] At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.165 g, 8.4 mmol) in THF (50 mL) was added dropwise to a solution of 1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)ethanone 4a (2.68 g, 8 mmol) in THF (50 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was dissolved in EtOAc and washed with water. A precipitate appeared and the solids were filtered off and dried under vacuum to give 2-bromo-1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)ethanone 4b (2.75 g).

[0127] Synthesis of Compound 4 and Chiral Separation into Enantiomers 4A and 4B:

[0128] A mixture of 2-bromo-1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)-ethanone 4b (2.3 g, 5.6 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (1.53 g, 8.4 mmol) and diisopropylethylamine (2.4 mL, 13.9 mmol) in CH.sub.3CN/THF (1/1) (140 mL) was stirred at 50° C. for 12 h. The mixture was concentrated under reduced pressure. The residue was diluted with EtOAc, washed with 1N HCl, and then with water. The organic layer was dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel (15-40 μm, 80 g in CH.sub.2Cl.sub.2/MeOH (99.5/0.5)). The pure fractions were collected and evaporated under reduced pressure. A small amount was crystallized from Et.sub.2O/CH.sub.3CN to give an analytical sample of 1-(6-chloro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 4) as a racemic mixture. The remaining amount of Compound 4 (2.1 g) was further purified via preparative LC (Stationary phase: irregular bare silica 150 g, mobile phase: toluene/iPrOH 95/5).

[0129] The Enantiomers of Compound 4 (1.9 g) were separated via Preparative Chiral SFC (Stationary phase: Chiralpak® IC 5 μm 250×30 mm, Mobile phase: 60% CO.sub.2, 40% MeOH) to give 870 mg of the first eluted enantiomer and 870 mg of the second eluted enantiomer. The two enantiomers were purified again by flash chromatography on silica gel (15-40 μm, 24 g in CH.sub.2Cl.sub.2/MeOH (99.5/0.5)). The first eluted enantiomer (800 mg) was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 4A (693 mg) as an amorphous white powder. The second eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 4B (619 mg) as an amorphous white powder.

[0130] Compound 4:

[0131] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.60 (s, 3H) 3.64 (t, J=5.0 Hz, 2H) 3.75-3.88 (m, 2H) 3.95 (s, 3H) 4.38-5.09 (m, 1H) 5.71 (t, J=1.9 Hz, 1H) 5.93 (d, J=2.2 Hz, 2H) 6.13-6.18 (m, 1H) 6.35-6.46 (m, 1H) 6.97 (dd, J=8.4, 1.9 Hz, 1H) 7.09 (d, J=2.2 Hz, 1H) 7.21 (dd, J=8.5, 1.9 Hz, 1H) 7.35 (d, J=8.4 Hz, 1H) 7.53 (d, J=1.9 Hz, 1H) 8.13 (d, J=8.5 Hz, 1H) 8.46 (d, J=2.8 Hz, 1H) 12.13 (d, J=2.8 Hz, 1H)

[0132] LC/MS (method LC-C): R.sub.t 3.11 min, MH.sup.+ 515

[0133] Melting point: 154° C.

[0134] Enantiomer 4A:

[0135] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.60 (s, 3H) 3.64 (q, J=5.0 Hz, 2H) 3.74-3.88 (m, 2H) 3.95 (s, 3H) 4.79 (t, J=5.0 Hz, 1H) 5.71 (t, J=2.0 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.15 (d, J=7.9 Hz, 1H) 6.41 (d, J=8.2 Hz, 1H) 6.97 (dd, J=8.2, 1.9 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.21 (dd, J=8.5, 1.9 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.53 (d, J=1.9 Hz, 1H) 8.13 (d, J=8.5 Hz, 1H) 8.46 (s, 1H) 12.12 (br. s., 1H)

[0136] LC/MS (method LC-C): R.sub.t 3.13 min, MH.sup.+ 515

[0137] [α].sub.D.sup.20: +111.6° (c 0.284, DMF)

[0138] Chiral SFC (method SFC-A): R.sub.t 3.68 min, MH.sup.+ 515, chiral purity 100%.

[0139] Enantiomer 4B:

[0140] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.62-3.68 (m, 2H) 3.76-3.89 (m, 2H) 3.95 (s, 3H) 4.74-4.83 (m, 1H) 5.72 (t, J=2.0 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.15 (d, J=7.9 Hz, 1H) 6.42 (d, J=8.2 Hz, 1H) 6.97 (dd, J=8.2, 1.9 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.21 (dd, J=8.5, 1.9 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.53 (d, J=1.9 Hz, 1H) 8.13 (d, J=8.5 Hz, 1H) 8.46 (s, 1H) 12.13 (br. s., 1H)

[0141] LC/MS (method LC-C): R.sub.t 3.14 min, MH.sup.+ 515

[0142] [α].sub.D.sup.20: −113.9° (c 0.288, DMF)

[0143] Chiral SFC (method SFC-A): R.sub.t 5.04 min, MH.sup.+ 515, chiral purity 100%.

Example 5: Synthesis 2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)-1-(6-methoxy-1H-indol-3-yl)ethanone (Compound 5) and Chiral Separation into Enantiomers 5A and 5B

[0144] ##STR00014## ##STR00015##

[0145] Synthesis of Intermediate 5a:

[0146] A solution of NaHSO.sub.3 (5.7 g, 54.5 mmol) in water (45 mL) was added to a stirring solution of tert-butyl 3-formyl-6-methoxy-1H-indole-1-carboxylate [CAS 847448-73-1] (10 g, 36.3 mmol) in dioxane (45 mL). After 15 min, morpholine (4.8 mL, 54.5 mmol) was added and 35 min later, sodium cyanide (NaCN) (1.96 g, 40 mmol) was added. The resulting suspension was stirred at room temperature for 3 days, until completion of the reaction. The product was filtered off and washed with a 1/1 mixture of dioxane/water (3×35 mL) and subsequently with water (3×45 mL) and dried under vacuum at 60° C. The solids were stirred up in Et.sub.2O (125 mL), filtered off, washed with Et.sub.2O (3×) and dried under vacuum at 50° C. to provide tert-butyl 3-(cyano(morpholino)methyl)-6-methoxy-1H-indole-1-carboxylate 5a (12.3 g).

[0147] Synthesis of Intermediate 5b:

[0148] A mixture of tert-butyl 3-(cyano(morpholino)methyl)-6-methoxy-1H-indole-1-carboxylate 5a (6.0 g, 16.2 mmol) in dry DMF (80 mL) was stirred under N.sub.2-atmosphere while cooling on an ice-bath. A solution of KHMDS 0.5 M in toluene (35.5 mL, 17.8 mmol) was added dropwise over 10 min. After stirring for an additional 10 min, 4-chloro-1-(chloromethyl)-2-methoxybenzene [CAS 101079-84-9] (3.09 g, 16.2 mmol) was added and the resulting mixture was stirred at room temperature for 20 h. The reaction mixture was poured into cold water (400 mL) and the product was extracted with Et.sub.2O (2×). The combined organic layers were washed with brine, dried over MgSO.sub.4, filtered, evaporated under reduced pressure and co-evaporated with xylene. The residue was purified by flash chromatography (Stationary phase: Biotage® Grace Reveleris Silica 120 g, Mobile phase: heptane/EtOAc gradient 100/0 to 20/80). The desired fractions were combined, evaporated under reduced pressure and co-evaporated with dioxane to give tert-butyl 3-(2-(4-chloro-2-methoxyphenyl)-1-cyano-1-morpholinoethyl)-6-methoxy-1H-indole-1-carboxylate 5b (7.75 g).

[0149] Synthesis of Intermediate 5c:

[0150] To a stirred suspension of tert-butyl 3-(2-(4-chloro-2-methoxyphenyl)-1-cyano-1-morpholinoethyl)-6-methoxy-1H-indole-1-carboxylate 5b (7.75 g, 14.7 mmol) in dioxane (40 mL) and water (20 mL) was added a solution of HCl 6M in isopropanol (36.8 mL, 220 mmol). The resulting mixture was stirred at 60° C. for 4 h and subsequently at 80° C. for 1 hour. After cooling to room temperature, the mixture was left standing for 20 h to allow crystallization of the reaction product. The product was filtered off, washed with a 1/1/1 mixture of iPrOH/H.sub.2O/dioxane (2×15 mL) and dried under vacuum at 50° C. to give 2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-1H-indol-3-yl)ethanone 5c (3.67 g).

[0151] Synthesis of Compound 5 and Chiral Separation into Enantiomers 5A and 5B:

[0152] A stirred mixture of 2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-1H-indol-3-yl)-ethanone 5c (2.35 g, 7.13 mmol) in THF (100 mL) was cooled on an ice-bath under N.sub.2-atm. Phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.81 g, 7.48 mmol) was added and the reaction mixture was stirred at 0° C. for 1 h and subsequently at room temperature for 1.5 h. 2-(3-Amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (2.61 g, 14.3 mmol), diisopropylethylamine (2.46 mL, 14.3 mmol) and CH.sub.3CN (100 mL) were added and the reaction mixture was stirred at room temperature for 18 h and at 55° C. for 2 h. The reaction mixture was concentrated under reduced pressure to 50% of the original volume. 2-(3-Amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (1 g) and diisopropylethylamine (1.5 mL) were added and the reaction mixture was stirred at room temperature for 65 h. The reaction mixture was poured out into water (400 mL) and the product was extracted with 2-MeTHF (2×). The combined organic layers were washed with brine, dried over MgSO.sub.4, filtered and evaporated under reduced pressure. The residue (7 g) was purified by flash chromatography (stationary phase: Biotage® Grace Reveleris Silica 120 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 50/37/13). The desired fractions were combined and evaporated under reduced pressure. The residue (5.8 g) was further purified by Preparative HPLC (Stationary phase: Uptisphere® C18 ODB—10 μm, 200 g, 5 cm, Mobile phase: 0.25% NH.sub.4HCO.sub.3 solution in water, CH.sub.3CN). The product fractions were combined and evaporated under reduced pressure. The residue was dissolved in MeOH (20 mL) and left standing to crystallize for 4 h. the solids were filtered off, washed with MeOH (3×5 mL) and dried under vacuum at 50° C. to provide 2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)-1-(6-methoxy-1H-indol-3-yl)ethanone (Compound 5, 2.06 g) as a racemic mixture.

[0153] Chiral separation of Compound 5 (2 g) was performed via Normal Phase Chiral separation (Stationary phase: AD-H, Mobile phase: 50% methanol, 50% ethanol). The product fractions were combined and evaporated under reduced pressure. The first eluted enantiomer was crystallized from a stirring solution of MeOH (13 mL) by addition of water (3 mL). After overnight stirring, the solids were filtered off, washed with a 3/1 mixture of MeOH/H.sub.2O (4×3 mL) and dried under vacuum at 50° C. to provide Enantiomer 5A (476 mg). The second eluted enantiomer was crystallized from a stirring solution of MeOH (13 mL) by addition of water (3 mL). After overnight stirring, the solids were filtered off, washed with a 3/1 mixture of MeOH/H.sub.2O (4×3 mL) and dried under vacuum at 50° C. to provide Enantiomer 5B (362 mg).

[0154] Compound 5:

[0155] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.65 (q, J=5.3 Hz, 2H) 3.77 (s, 3H) 3.78-3.90 (m, 2H) 3.97 (s, 3H) 4.77 (t, J=5.6 Hz, 1H) 5.71 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.2 Hz, 2H) 6.13 (d, J=8.1 Hz, 1H) 6.36 (d, J=7.9 Hz, 1H) 6.83 (dd, J=8.7, 2.3 Hz, 1H) 6.92-7.00 (m, 2H) 7.09 (d, J=2.0 Hz, 1H) 7.36 (d, J=8.4 Hz, 1H) 8.01 (d, J=8.6 Hz, 1H) 8.29 (d, J=2.9 Hz, 1H) 11.81 (br d, J=2.2 Hz, 1H)

[0156] LC/MS (method LC-B): R.sub.t 1.93, MH.sup.+ 511

[0157] Enantiomer 5A:

[0158] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.4 Hz, 2H) 3.77 (s, 3H) 3.78-3.90 (m, 2H) 3.97 (s, 3H) 4.77 (t, J=5.5 Hz, 1H) 5.71 (t, J=2.0 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.13 (d, J=8.1 Hz, 1H) 6.36 (d, J=7.9 Hz, 1H) 6.83 (dd, J=8.7, 2.3 Hz, 1H) 6.92-6.99 (m, 2H) 7.09 (d, J=2.0 Hz, 1H) 7.36 (d, J=8.4 Hz, 1H) 8.01 (d, J=8.8 Hz, 1H) 8.29 (d, J=3.1 Hz, 1H) 11.81 (br d, J=2.4 Hz, 1H)

[0159] LC/MS (method LC-A): R.sub.t 1.08, MH.sup.+ 511

[0160] [α].sub.D.sup.20: +109.3° (c 0.61, DMF)

[0161] Chiral SFC (method SFC-G): R.sub.t 1.78 min, MH.sup.+ 511, chiral purity 100%

[0162] Enantiomer 5B:

[0163] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.2 Hz, 2H) 3.77 (s, 3H) 3.78-3.89 (m, 2H) 3.97 (s, 3H) 4.77 (t, J=5.6 Hz, 1H) 5.71 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.12 (d, J=7.9 Hz, 1H) 6.35 (d, J=7.9 Hz, 1H) 6.82 (dd, J=8.7, 2.3 Hz, 1H) 6.91-7.01 (m, 2H) 7.09 (d, J=2.0 Hz, 1H) 7.36 (d, J=8.1 Hz, 1H) 8.01 (d, J=8.6 Hz, 1H)

[0164] LC/MS (method LC-A): R.sub.t 1.08, MH.sup.+ 511

[0165] [α].sub.D.sup.20: −108.9° (c 0.52, DMF)

[0166] Chiral SFC (method SFC-G): R.sub.t 2.19 min, MH.sup.+ 511, chiral purity 100%

Example 6: Synthesis of 2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone (Compound 6) and Chiral Separation into Enantiomers 6A and 6B

[0167] ##STR00016##

[0168] Synthesis of Intermediate 6a:

[0169] Diethylaluminum chloride 1M in hexane (13.5 mL, 13.5 mmol) was added dropwise at 0° C. to a solution of 6-methoxy-5-methyl-1H-indole [CAS 1071973-95-9] (1.45 g, 9 mmol) in CH.sub.2Cl.sub.2 (45 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (2.4 g, 10.9 mmol, synthesis: see example 1) in CH.sub.2Cl.sub.2 (45 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. Ice-water was added and the precipitate was filtered off and washed with water. The solid was dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone 6a (2.1 g).

[0170] Synthesis of Intermediate 6b:

[0171] At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.4 g, 6.4 mmol) in THF (65 mL) was added dropwise to a mixture of 2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone 6a (2.1 g, 6.1 mmol) in THF (60 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 2.5 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with the minimum of diisopropylether. The precipitate was filtered off and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone 6b (2.36 g).

[0172] Synthesis of Compound 6 and Chiral Separation into Enantiomers 6A and 6B:

[0173] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone 6b (1.35 g, 3.2 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (0.585 g, 3.2 mmol) and diisopropylethylamine (0.83 mL, 4.8 mmol) in CH.sub.3CN/THF (1/1) (80 mL) was stirred at 70° C. for 24 h. The mixture was concentrated under reduced pressure. The residue was diluted with CH.sub.2Cl.sub.2, washed with 1N HCl and water. The organic layer was separated, dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The crude compound was purified by column chromatography on silica gel (15-40 μm, 80 g in CH.sub.2Cl.sub.2/MeOH (99.5/0.5)). A small amount was crystallized from Et.sub.2O/CH.sub.3CN to give an analytical sample of 2-(4-chloro-2-methoxyphenyl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)-1-(6-methoxy-5-methyl-1H-indol-3-yl)ethanone (Compound 3) as a racemic mixture. The remaining amount of crude Compound 3 was mixed with another batch (total amount 1.19 g) and was further purified twice via preparative LC (Stationary phase: irregular bare silica 150 g, Mobile phase: CH.sub.2Cl.sub.2/MeOH (98/2), and then toluene/iPrOH (95/5).

[0174] The Enantiomers of Compound 3 (950 mg) were separated via Preparative Chiral SFC (Stationary phase: Chiralpak® IC 5 μm 250×30 mm, Mobile phase: 50% CO.sub.2, 50% MeOH) to give 485 mg of the first eluted enantiomer and 480 mg of the second eluted enantiomer. The first eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 6A (406 mg) as an amorphous white powder. The second eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 6B (436 mg) as an amorphous white powder.

[0175] Compound 6:

[0176] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.21 (s, 3H) 3.61 (s, 3H) 3.62-3.68 (m, 2H) 3.74-3.90 (m, 5H) 3.97 (s, 3H) 4.76 (t, J=4.8 Hz, 1H) 5.68-5.74 (m, 1H) 5.93 (d, J=1.5 Hz, 2H) 6.11 (d, J=7.6 Hz, 1H) 6.31 (d, J=7.6 Hz, 1H) 6.92 (s, 1H) 6.95 (dd, J=8.3, 1.8 Hz, 1H) 7.09 (d, J=1.8 Hz, 1H) 7.35 (d, J=8.3 Hz, 1H) 7.89 (s, 1H) 8.22 (s, 1H) 11.73 (br. s., 1H)

[0177] LC/MS (method LC-C): R.sub.t 3.02 min, MH.sup.+ 525

[0178] Enantiomer 6A:

[0179] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 2.20 (s, 3H) 3.60 (s, 3H) 3.64 (q, J=5.3 Hz, 2H) 3.75-3.88 (m, 5H) 3.97 (s, 3H) 4.78 (t, J=5.3 Hz, 1H) 5.70 (t, J=2.0 Hz, 1H) 5.92 (d, J=2.0 Hz, 2H) 6.11 (d, J=7.9 Hz, 1H) 6.33 (d, J=7.9 Hz, 1H) 6.92 (s, 1H) 6.95 (dd, J=8.2, 1.9 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.88 (s, 1H) 8.23 (s, 1H) 11.75 (br. s., 1H)

[0180] LC/MS (method LC-C): R.sub.t 3.04 min, MH.sup.+ 525

[0181] [α].sub.D.sup.20: +116.8° (c 0.4536, DMF)

[0182] Chiral SFC (method SFC-B): R.sub.t 2.40 min, MH.sup.+ 525, chiral purity 100%.

[0183] Enantiomer 6B:

[0184] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 2.20 (s, 3H) 3.60 (s, 3H) 3.64 (q, J=5.4 Hz, 2H) 3.77-3.88 (m, 5H) 3.97 (s, 3H) 4.78 (t, J=5.4 Hz, 1H) 5.70 (t, J=2.0 Hz, 1H) 5.92 (d, J=2.0 Hz, 2H) 6.11 (d, J=7.9 Hz, 1H) 6.33 (d, J=7.9 Hz, 1H) 6.92 (s, 1H) 6.95 (dd, J=8.2, 1.9 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.88 (s, 1H) 8.23 (s, 1H) 11.75 (br. s., 1H)

[0185] LC/MS (method LC-C): R.sub.t 3.04 min, MH.sup.+ 525

[0186] [α].sub.D.sup.20: −121.9° (c 0.3855, DMF)

[0187] Chiral SFC (method SFC-B): R.sub.t 3.75 min, MH.sup.+ 525, chiral purity 99.86%.

Example 7: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 7) and Chiral Separation into Enantiomers 7A and 7B

[0188] ##STR00017##

[0189] Synthesis of Intermediate 7a:

[0190] Diethylaluminum chloride 1M in hexane (15.7 mL, 15.7 mmol) was added dropwise at 0° C. to a solution of 5-fluoro-6-methoxy-1H-indole [CAS 1211595-72-0] (2 g, 12.1 mmol) in CH.sub.2Cl.sub.2 (50 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.2 g, 14.6 mmol, synthesis: see example 1) in CH.sub.2Cl.sub.2 (50 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. Ice-water was added and the precipitate was filtered off, washed with water and the minimum of CH.sub.2Cl.sub.2. The solid was dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)ethanone 7a (2.82 g).

[0191] Synthesis of Intermediate 7b:

[0192] At 0° C., a solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.5 g, 8.1 mmol) in THF (20 mL) was added dropwise to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)ethanone 7a (2.82 g, 8.1 mmol) in THF (46 mL). The mixture was stirred at 0° C. for 1 h and at room temperature for 4 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was dissolved in EtOAc, washed with water. The organic phase was dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The residue was taken up with the minimum of EtOAc. The precipitate was filtered off and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)ethanone 7b (2.5 g).

[0193] Synthesis of Compound 7 and Chiral Separation into Enantiomers 7A and 7B:

[0194] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)ethanone 7b (2.4 g, 5.6 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (1.5 g, 8.2 mmol) and diisopropylethylamine (1.45 mL, 8.4 mmol) in CH.sub.3CN/THF (1/1) (48 mL) was stirred at 50° C. for 12 h. The mixture was concentrated under reduced pressure. The residue was diluted with EtOAc, washed with 1N HCl and water. The organic layer was separated, dried over MgSO.sub.4, filtered and the solvent was evaporated under reduced pressure. The residue was purified by column chromatography on silica gel (15-40 μm, 80 g in CH.sub.2Cl.sub.2/MeOH (99.5/0.5)). The pure fractions were collected and evaporated under reduced pressure. A small amount was solidified from Et.sub.2O/CH.sub.3CN to give an analytical sample of 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-6-methoxy-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 7) as a racemic mixture.

[0195] The Enantiomers of Compound 7 (1.6 g) were separated via Preparative Chiral SFC (Stationary phase: Chiralpak® IC 5 μm 250×30 mm, Mobile phase: 55% CO.sub.2, 45% MeOH) to give 680 mg of the first eluted enantiomer and 720 mg of the second eluted enantiomer. The first eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 7A (603 mg) as an amorphous white powder. The second eluted enantiomer was solidified from CH.sub.3CN/Et.sub.2O to afford Enantiomer 7B (505 mg) as an amorphous white powder.

[0196] Compound 7:

[0197] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.60 (s, 3H) 3.62-3.67 (m, 2H) 3.74-3.88 (m, 5H) 3.96 (s, 3H) 4.77 (t, J=5.3 Hz, 1H) 5.66-5.75 (m, 1H) 5.92 (d, J=1.8 Hz, 2H) 6.12 (d, J=8.1 Hz, 1H) 6.37 (d, J=8.1 Hz, 1H) 6.96 (dd, J=8.1, 1.8 Hz, 1H) 7.09 (d, J=1.8 Hz, 1H) 7.14 (d, J=7.6 Hz, 1H) 7.35 (d, J=8.1 Hz, 1H) 7.81 (d, J=11.6 Hz, 1H) 8.33 (s, 1H) 11.94 (br. s., 1H)

[0198] LC/MS (method LC-C): R.sub.t 2.90 min, MH.sup.+ 529

[0199] Enantiomer 7A:

[0200] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.60 (s, 3H) 3.64 (q, J=5.5 Hz, 2H) 3.76-3.88 (m, 5H) 3.96 (s, 3H) 4.79 (t, J=5.5 Hz, 1H) 5.71 (t, J=1.9 Hz, 1H) 5.92 (d, J=1.9 Hz, 2H) 6.12 (d, J=8.2 Hz, 1H) 6.39 (d, J=8.2 Hz, 1H) 6.96 (dd, J=8.2, 2.0 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.14 (d, J=7.6 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.81 (d, J=11.7 Hz, 1H) 8.34 (s, 1H) 11.95 (br. s., 1H)

[0201] LC/MS (method LC-C): R.sub.t 2.90 min, MH.sup.+ 529

[0202] [α].sub.D.sup.20: +86.2° (c 0.232, DMF)

[0203] Chiral SFC (method SFC-B): R.sub.t 2.28 min, MH.sup.+ 529, chiral purity 100%.

[0204] Enantiomer 7B:

[0205] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 3.60 (s, 3H) 3.64 (q, J=5.5 Hz, 2H) 3.76-3.88 (m, 5H) 3.96 (s, 3H) 4.79 (t, J=5.5 Hz, 1H) 5.71 (t, J=1.9 Hz, 1H) 5.92 (d, J=1.9 Hz, 2H) 6.12 (d, J=8.2 Hz, 1H) 6.39 (d, J=8.2 Hz, 1H) 6.96 (dd, J=8.2, 1.9 Hz, 1H) 7.09 (d, J=1.9 Hz, 1H) 7.14 (d, J=7.6 Hz, 1H) 7.35 (d, J=8.2 Hz, 1H) 7.81 (d, J=12.0 Hz, 1H) 8.34 (s, 1H) 11.95 (br. s., 1H)

[0206] LC/MS (method LC-C): R.sub.t 2.90 min, MH.sup.+ 529

[0207] [α].sub.D.sup.20: −88.7° (c 0.3, DMF)

[0208] Chiral SFC (method SFC-B): R.sub.t 4.04 min, MH.sup.+ 529, chiral purity 100%.

Example 8: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 8) and Chiral Separation into Enantiomers 8A and 8B

[0209] ##STR00018##

[0210] Synthesis of Intermediate 8a:

[0211] Diethylaluminum chloride 1M in hexane (15.0 mL, 15.0 mmol) was added dropwise at 0° C. to a solution of 5-fluoro-7-methyl-1H-indole [CAS 1082041-52-8] (1.49 g, 10.0 mmol) in CH.sub.2Cl.sub.2 (20 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.28 g, 15.0 mmol, synthesis: see Example 1) in CH.sub.2Cl.sub.2 (10 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. 1M Rochelle salt solution was added and the reaction mixture was stirred at room temperature for 30 min. The formed solids were filtered off and partitioned between EtOAc and 1N HCl. The phases were separated. The aqueous layer was extracted with EtOAc. The organic phases were combined, washed with brine, dried over MgSO.sub.4, filtered and concentrated under reduced pressure to give 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)ethanone 8a (2.03 g).

[0212] Synthesis of Intermediate 8b:

[0213] A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.53 g, 6.72 mmol) in THF (10 mL) was added dropwise at 0° C. to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)ethanone 8a (2.03 g, 6.11 mmol) in THF (50 mL). The reaction mixture was stirred at room temperature overnight. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with a minimum of acetonitrile. The precipitate was filtered off, washed with acetonitrile and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)ethanone 8b (2.00 g).

[0214] Synthesis of Compound 8 and Chiral Separation into Enantiomers 8A and 8B: A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)ethanone 8b (1.70 g, 4.14 mmol) and 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (2.28 g, 12.4 mmol) in THF (10 mL) and CH.sub.3CN (10 mL) was stirred at room temperature for 3 days. The reaction mixture was concentrated under reduced pressure. The residue was partitioned between EtOAc and 1N HCl. The phases were separated. The organic phase was washed twice with 1N HCl, with an aqueous saturated NaHCO.sub.3 solution and brine, dried over MgSO.sub.4, filtered and concentrated under reduced pressure. The residue was taken up with a minimum amount of acetonitrile. The precipitate was filtered off and dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(5-fluoro-7-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethan-one (Compound 8, 1.23 g) as a racemic mixture.

[0215] The enantiomers of Compound 8 (1.17 g) were separated via Normal Phase Chiral separation (Stationary phase: Daicel Chiralpak® OD-H, Mobile phase: 80% heptane, 20% ethanol). The first eluted product was further purified by flash chromatography (Stationary phase: Biotage® Grace Reveleris Silica 12 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The pure fractions were combined and evaporated. The product was crystallized overnight from a mixture of MeOH (4 mL) and water (1 mL), filtered off, washed with MeOH (3×) and dried under vacuum at 50° C. to provide Enantiomer 8A (37 mg). The second eluted product was further purified by Flash Chromatography (Stationary phase: Biotage® Grace Reveleris Silica 12 g, Mobile phase: heptane/EtOAc/EtOH gradient 100/0/0 to 40/45/15). The pure fractions were combined and evaporated. The product was crystallized from a mixture of MeOH and H.sub.2O, filtered off, washed with MeOH and dried under vacuum at 50° C. to provide Enantiomer 8B (177 mg).

[0216] Compound 8:

[0217] .sup.1H NMR (300 MHz, DMSO-d.sub.6) δ ppm 2.48 (s, 3H) 3.56-3.71 (m, 5H) 3.74-3.92 (m, 2H) 3.97 (s, 3H) 4.79 (t, J=5.5 Hz, 1H) 5.72 (s, 1H) 5.95 (d, J=1.9 Hz, 2H) 6.17 (d, J=7.9 Hz, 1H) 6.40 (d, J=8.3 Hz, 1H) 6.87-7.01 (m, 2H) 7.10 (d, J=1.9 Hz, 1H) 7.36 (d, J=8.3 Hz, 1H) 7.65 (dd, J=9.8, 2.3 Hz, 1H) 8.46 (s, 1H) 12.22 (br. s., 1H)

[0218] LC/MS (method LC-D): R.sub.t 1.52 min, MH.sup.+ 513

[0219] Enantiomer 8A:

[0220] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.2 Hz, 2H) 3.77 (s, 3H) 3.78-3.89 (m, 2H) 3.97 (s, 3H) 4.77 (t, J=5.6 Hz, 1H) 5.71 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.12 (d, J=7.9 Hz, 1H) 6.35 (d, J=7.9 Hz, 1H) 6.82 (dd, J=8.7, 2.3 Hz, 1H) 6.91-7.01 (m, 2H) 7.09 (d, J=2.0 Hz, 1H) 7.36 (d, J=8.1 Hz, 1H) 8.01 (d, J=8.6 Hz, 1H) 8.29 (d, J=2.9 Hz, 1H) 11.81 (br d, J=2.4 Hz, 1H)

[0221] LC/MS (method LC-A): R.sub.t 1.12 min, MH.sup.+ 513

[0222] [α].sub.D.sup.20: −83.8° (c 0.4725, DMF)

[0223] Chiral SFC (method SFC-G): R.sub.t 2.32 min, MH.sup.+ 513, chiral purity 100%

[0224] Enantiomer 8B:

[0225] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.47 (s, 3H) 3.61 (s, 3H) 3.65 (q, J=5.2 Hz, 2H) 3.77-3.90 (m, 2H) 3.97 (s, 3H) 4.77 (t, J=5.6 Hz, 1H) 5.72 (t, J=2.1 Hz, 1H) 5.95 (d, J=2.0 Hz, 2H) 6.16 (d, J=8.1 Hz, 1H) 6.37 (d, J=7.9 Hz, 1H) 6.92 (dd, J=10.1, 2.0 Hz, 1H) 6.96 (dd, J=8.3, 1.9 Hz, 1H) 7.10 (d, J=1.8 Hz, 1H) 7.36 (d, J=8.4 Hz, 1H) 7.65 (dd, J=9.7, 2.4 Hz, 1H) 8.45 (d, J=3.5 Hz, 1H) 12.20 (br d, J=2.9 Hz, 1H)

[0226] LC/MS (method LC-A): R.sub.t 1.14 min, MH.sup.+ 513

[0227] [α].sub.D.sup.20: +86.6° (c 0.4805, DMF)

[0228] Chiral SFC (method SFC-G): R.sub.t 1.44 min, MH.sup.+ 513, chiral purity 100%

Example 9: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 9) and Chiral Separation into Enantiomers 9A and 9B

[0229] ##STR00019##

[0230] Synthesis of Intermediate 9a:

[0231] Diethylaluminum chloride 1M in hexane (14.9 mL, 14.9 mmol) was added dropwise at 0° C. to a solution of 5,6-difluoro-1H-indole [CAS 169674-01-5] (1.50 g, 9.8 mmol) in CH.sub.2Cl.sub.2 (20 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.22 g, 14.7 mmol, synthesis: see Example 1) in CH.sub.2Cl.sub.2 (20 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 2 h. 1M Rochelle salt solution was added and the reaction mixture was vigorously stirred at room temperature for 2 h. The precipitate was filtered off and partitioned between EtOAc and 1N HCl. The phases were separated. The aqueous layer was extracted twice with EtOAc. The organic phases were combined, dried over MgSO.sub.4, filtered and concentrated under reduced pressure. The residue was taken up with a minimum of acetonitrile. The precipitate was filtered off and dried under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)ethanone 9a (2.73 g).

[0232] Synthesis of Intermediate 9b:

[0233] A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (3.36 g, 8.94 mmol) in THF (50 mL) was added dropwise at 0° C. to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)-2-(4-chloro-2-methoxyphenyl)ethanone 9a (2.73 g, 8.13 mmol) in THF (80 mL). The reaction mixture was stirred at 0° C. for 15 min and at room temperature for 2 h. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with a minimum of acetonitrile. The precipitate was filtered off and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)ethanone 9b (3.00 g).

[0234] Synthesis of Compound 9 and Chiral Separation into Enantiomers 9A and 9B:

[0235] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)ethanone 9b (1.80 g, 4.34 mmol) and 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (2.39 g, 13.0 mmol) in THF (9 mL) and CH.sub.3CN (9 mL) was stirred at room temperature for 65 h. 1N HCl was added and the reaction mixture was extracted with EtOAc. The phases were separated. The organic phase was washed with 1N HCl, dried over MgSO.sub.4, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (15% to 70%) in dichloromethane. The pure fractions were combined and concentrated under reduced pressure to afford 2-(4-chloro-2-methoxyphenyl)-1-(5,6-difluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 9, 1.20 g) as a racemic mixture. The impure fractions were combined and purified by flash chromatography on silica gel using a gradient of ethyl acetate (15% to 70%) in dichloromethane to afford a second batch of Compound 9 (0.290 g) as a racemic mixture.

[0236] The enantiomers of Compound 9 (1.4 g) were separated via Normal Phase Chiral separation (Stationary phase: Daicel Chiralpak® OD-H, Mobile phase: 80% heptane, 20% ethanol). The first eluted product was further purified by flash chromatography (Stationary phase: Biotage® Grace Reveleris Silica 12 g, Mobile phase: CH.sub.2Cl.sub.2/MeOH gradient from 100/0 to 90/10). The pure fractions were combined, evaporated and co-evaporated with MeOH. The residue was lyophilized from a solvent mixture of CH.sub.3CN (4.5 mL) and water (2.5 mL) and dried under vacuum at 45° C. to provide Enantiomer 9A (409 mg). The second eluted product was further purified by flash chromatography (Stationary phase: Biotage® Grace Reveleris Silica 12 g, Mobile phase: CH.sub.2Cl.sub.2/MeOH gradient from 100/0 to 90/10). The pure fractions were combined, evaporated and co-evaporated with MeOH. The residue was lyophilized from a solvent mixture of CH.sub.3CN (5 mL) and water (3 mL) and dried under vacuum at 45° C. to provide Enantiomer 9B (388 mg).

[0237] Compound 9:

[0238] .sup.1H NMR (300 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.62-3.70 (m, 2H) 3.75-3.90 (m, 2H) 3.95 (s, 3H) 4.79 (t, J=5.6 Hz, 1H) 5.72 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.0 Hz, 1H) 6.15 (d, J=8.1 Hz, 1H) 6.42 (d, J=8.1 Hz, 1H) 6.97 (dd, J=8.2, 2.2 Hz, 1H) 7.10 (d, J=2.0 Hz, 1H) 7.35 (d, J=8.3 Hz, 1H) 7.54 (dd, J=10.8, 7.0 Hz, 1H) 7.99 (dd, J=11.2, 8.2 Hz, 1H) 8.48 (s, 1H) 12.19 (br. s., 1H)

[0239] LC/MS (method LC-D): R.sub.t 1.44 min, MH.sup.+ 517

[0240] Enantiomer 9A:

[0241] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.2 Hz, 2H) 3.77-3.89 (m, 2H) 3.95 (s, 3H) 4.77 (t, J=5.5 Hz, 1H) 5.72 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.0 Hz, 2H) 6.14 (d, J=8.1 Hz, 1H) 6.39 (d, J=8.1 Hz, 1H) 6.97 (dd, J=8.4, 2.0 Hz, 1H) 7.09 (d, J=2.0 Hz, 1H) 7.35 (d, J=8.1 Hz, 1H) 7.53 (dd, J=10.8, 7.0 Hz, 1H) 7.99 (dd, J=11.2, 8.1 Hz, 1H) 8.47 (s, 1H) 12.16 (br s, 1H)

[0242] LC/MS (method LC-A): R.sub.t 1.15 min, MH.sup.+ 517

[0243] [α].sub.D.sup.20: −98.9° (c 0.445, DMF)

[0244] Chiral SFC (method SFC-G): R.sub.t 1.92 min, MH.sup.+ 517, chiral purity 100%

[0245] Enantiomer 9B:

[0246] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 3.61 (s, 3H) 3.64 (q, J=5.2 Hz, 2H) 3.75-3.91 (m, 2H) 3.95 (s, 3H) 4.77 (t, J=5.6 Hz, 1H) 5.72 (t, J=2.1 Hz, 1H) 5.93 (d, J=2.2 Hz, 2H) 6.14 (d, J=8.1 Hz, 1H) 6.39 (d, J=8.1 Hz, 1H) 6.97 (dd, J=8.1, 2.0 Hz, 1H) 7.09 (d, J=2.0 Hz, 1H) 7.35 (d, J=8.1 Hz, 1H) 7.53 (dd, J=10.8, 7.0 Hz, 1H) 7.99 (dd, J=11.1, 8.3 Hz, 1H) 8.47 (s, 1H) 12.16 (br s, 1H)

[0247] LC/MS (method LC-A): R.sub.t 1.15 min, MH.sup.+ 517

[0248] [α].sub.D.sup.20: +98.9° (c 0.555, DMF)

[0249] Chiral SFC (method SFC-G): R.sub.t 1.36 min, MH.sup.+ 517, chiral purity 100%

Example 10: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 10) and Chiral Separation into Enantiomers 10A and 10B

[0250] ##STR00020##

[0251] Synthesis of Intermediate 10a:

[0252] Diethylaluminum chloride 1M in hexane (16 mL, 16 mmol) was added dropwise at −15° C. to a solution of 7-fluoro-5-methyl-1H-indole [CAS 442910-91-0] (1.59 g, 10.7 mmol) in CH.sub.2Cl.sub.2 (150 mL) under N.sub.2 flow. After 15 min at −15° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.27 g, 14.9 mmol, synthesis: see Example 1) in CH.sub.2Cl.sub.2 (50 mL) was added slowly at −15° C. The reaction was stirred at −15° C. for 1 h and at room temperature for 2 h. The reaction mixture was poured out into a stirred ice/Rochelle salt mixture. The solids were removed from the reaction mixture by filtration over a short pad of Dicalite® and the filter cake was rinsed several times with THF. The layers were separated and the aqueous layer was extracted with THF. The combined organic layers were washed with brine, water, dried on MgSO.sub.4, filtered, and evaporated under reduced pressure. The solid residue was suspended in CH.sub.2Cl.sub.2 (10 mL). The solids were filtered off, washed with a small amount of CH.sub.2Cl.sub.2 and dried under vacuum at 50° C. to give 2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)ethanone 10a (2.22 g).

[0253] Synthesis of Intermediate 10b:

[0254] A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.77 g, 7.36 mmol) in THF (50 mL) was added dropwise at 0° C. to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)ethanone 10a (2.22 g, 6.7 mmol) in THF (150 mL). The reaction mixture was stirred at room temperature for 2 h. The precipitate was filtered off and washed with THF. The filtrate was concentrated under reduced pressure. The residue was taken up with a minimum of CH.sub.2Cl.sub.2. The precipitate was filtered off, washed with CH.sub.2Cl.sub.2 (2×) and dried under vacuum at 50° C. to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)ethanone 10b (2.55 g).

[0255] Synthesis of Compound 10 and Chiral Separation into Enantiomers 10A and 10B:

[0256] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)ethanone 10b (2 g, 4.87 mmol), 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (1.6 g, 7.3 mmol) and diisopropylethylamine (1.26 mL, 7.3 mmol) in CH.sub.3CN (100 mL) was stirred at room temperature for 20 h, at 60° C. for 8 h and again at room temperature for 16 h. The solvent was evaporated under reduced pressure. The residue was dissolved in CH.sub.2Cl.sub.2 (50 mL), washed with 0.5 M HCl (50 mL) and water (50 mL), dried over MgSO.sub.4, filtered and evaporated. The residue was purified by column chromatography (Stationary phase: Biotage® Grace Reveleris Silica 120 g, Mobile phase: EtOAc/heptane gradient from 0/100 to 60/40). The fractions containing product were combined, evaporated and dried at 50° C. under vacuum to give 2-(4-chloro-2-methoxyphenyl)-1-(7-fluoro-5-methyl-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 10, 1.3 g) as a white solid.

[0257] The enantiomers of Compound 10 (1.3 g) were separated via Normal Phase Chiral separation (Stationary phase: AD-H, Mobile phase: 70% ethanol, 30% methanol) to provide 637 mg of the first eluted enantiomer and 628 mg of the second eluted enantiomer. The first eluted product was crystallized from a MeOH/water mixture. The solids were filtered off and washed with a MeOH/water (1/1) mixture to provide Enantiomer 10A (302 mg) as a white amorphous powder. The second eluted product was further purified by flash chromatography (Stationary phase: Biotage® Grace Reveleris Silica 40 g, Mobile phase: MeOH/CH.sub.2Cl.sub.2 gradient from 0/100 to 2/98). The fractions containing product were combined and evaporated to provide Enantiomer 10B (335 mg) as a white amorphous powder.

[0258] Compound 10:

[0259] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.38 (s, 3H) 3.61 (s, 3H) 3.62-3.67 (m, 2H) 3.77-3.89 (m, 2H) 3.95 (s, 3H) 4.77 (t, J=5.5 Hz, 1H) 5.72 (t, J=2.0 Hz, 1H) 5.94 (d, J=2.0 Hz, 2H) 6.15 (d, J=8.1 Hz, 1H) 6.34 (d, J=8.1 Hz, 1H) 6.90 (d, J=12.1 Hz, 1H) 6.96 (dd, J=8.1, 2.0 Hz, 1H) 7.09 (d, J=2.0 Hz, 1H) 7.35 (d, J=8.4 Hz, 1H) 7.77 (s, 1H) 8.38 (s, 1H) 12.46 (br s, 1H)

[0260] LC/MS (method LC-A): R.sub.t 1.16 min, MH.sup.+ 513

[0261] Enantiomer 10A:

[0262] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.38 (s, 3H) 3.62 (s, 3H) 3.66 (br t, J=4.8 Hz, 2H) 3.77-3.92 (m, 2H) 3.96 (s, 3H) 4.78 (br s, 1H) 5.73 (s, 1H) 5.96 (s, 2H) 6.17 (br d, J=7.9 Hz, 1H) 6.35 (br d, J=8.1 Hz, 1H) 6.91 (d, J=12.1 Hz, 1H) 6.97 (dd, J=8.3, 1.4 Hz, 1H) 7.10 (d, J=1.5 Hz, 1H) 7.36 (d, J=8.4 Hz, 1H) 7.79 (s, 1H) 8.39 (s, 1H) 12.47 (br s, 1H)

[0263] LC/MS (method LC-A): R.sub.t 1.15 min, MH.sup.+ 513

[0264] [α].sub.D.sup.20: +132.3° (c 0.505, DMF)

[0265] Chiral SFC (method SFC-F): R.sub.t 2.13 min, MH.sup.+ 513, chiral purity 100%

[0266] Enantiomer 10B:

[0267] .sup.1H NMR (400 MHz, DMSO-d.sub.6) δ ppm 2.38 (s, 3H) 3.61 (s, 3H) 3.63-3.68 (m, 2H) 3.77-3.92 (m, 2H) 3.96 (s, 3H) 4.77 (br t, J=5.7 Hz, 1H) 5.72 (t, J=2.0 Hz, 1H) 5.95 (d, J=2.0 Hz, 2H) 6.16 (d, J=7.9 Hz, 1H) 6.35 (d, J=8.1 Hz, 1H) 6.91 (d, J=12.1 Hz, 1H) 6.97 (dd, J=8.4, 2.0 Hz, 1H) 7.09 (d, J=2.0 Hz, 1H) 7.36 (d, J=8.4 Hz, 1H) 7.78 (s, 1H) 8.39 (s, 1H) 12.46 (br s, 1H)

[0268] LC/MS (method LC-A): R.sub.t 1.15 min, MH.sup.+ 513

[0269] [α].sub.D.sup.20: −144.1° (c 0.4975, DMF)

[0270] Chiral SFC (method SFC-F): R.sub.t 3.13 min, MH.sup.+ 513, chiral purity 100%

Example 11: Synthesis of 2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 11) and Chiral Separation into Enantiomers 11A and 11B

[0271] ##STR00021##

[0272] Synthesis of Intermediate 11a:

[0273] Diethylaluminum chloride 1M in hexane (15.0 mL, 15.0 mmol) was added dropwise at 0° C. to a solution of 6,7-difluoro-1H-indole [CAS 271780-84-8] (1.53 g, 10.0 mmol) in CH.sub.2Cl.sub.2 (20 mL). After 30 min at 0° C., a solution of 2-(4-chloro-2-methoxyphenyl)acetyl chloride 1a (3.28 g, 15.0 mmol, synthesis: see Example 1) in CH.sub.2Cl.sub.2 (10 mL) was added slowly at 0° C. The reaction was stirred at 0° C. for 3 h. 1M Rochelle salt solution was added and the reaction mixture was stirred at room temperature for 30 min. The solids were filtered off and partitioned between EtOAc and 1N HCl. The phases were separated. The aqueous layer was extracted with EtOAc. The organic phases were combined, washed with brine, dried over MgSO.sub.4, filtered and concentrated under reduced pressure to give 2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)ethanone 11a (2.36 g).

[0274] Synthesis of Intermediate 11 b:

[0275] A solution of phenyltrimethylammonium tribromide [CAS 4207-56-1] (2.90 g, 7.02 mmol) in THF (10 mL) was added dropwise at 0° C. to a solution of 2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)ethanone 11a (2.36 g, 7.02 mmol) in THF (50 mL). The reaction mixture was stirred at room temperature overnight. The precipitate was filtered off and washed with EtOAc. The filtrate was concentrated under reduced pressure. The residue was taken up with a minimum of acetonitrile. The precipitate was filtered off, washed with acetonitrile and dried under vacuum to give 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)ethanone 11b (2.34 g).

[0276] Synthesis of Compound 11 and Chiral Separation into Enantiomers 11A and 11B:

[0277] A mixture of 2-bromo-2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)ethanone 11b (1.70 g, 4.14 mmol) and 2-(3-amino-5-methoxyphenoxy)ethanol [CAS 725237-16-1] (2.25 g, 12.3 mmol) in THF (10 mL) and CH.sub.3CN (10 mL) was stirred at room temperature for 3 days. The reaction mixture was concentrated under reduced pressure. The residue was partitioned between EtOAc and 1N HCl. The phases were separated. The organic phase was washed twice with 1N HCl, with an aqueous saturated NaHCO.sub.3 solution and brine, dried over MgSO.sub.4, filtered and concentrated under reduced pressure. The residue was purified by flash chromatography on silica gel using a gradient of EtOAc (20% to 100%) in heptane. The fractions containing expected compound were combined and concentrated under reduced pressure. The residue was crystallized from a mixture of Et.sub.2O, acetonitrile and heptane to afford 2-(4-chloro-2-methoxyphenyl)-1-(6,7-difluoro-1H-indol-3-yl)-2-((3-(2-hydroxyethoxy)-5-methoxyphenyl)amino)ethanone (Compound 11, 1.54 g) as a racemic mixture.

[0278] The enantiomers of Compound 11 (1.22 g) were separated via Preparative Chiral SFC (Stationary phase: Chiralcel® OD-H 5 μm 250×30 mm, Mobile phase: 55% CO.sub.2, 45% MeOH) to give 550 mg of the first eluted enantiomer and 570 mg of the second eluted enantiomer. The first eluted enantiomer was solidified from CH.sub.3CN/diisopropylether to afford Enantiomer 11A (487 mg). The second eluted enantiomer was solidified from CH.sub.3CN/diisopropylether to afford Enantiomer 11B (460 mg). Both enantiomers occurred as amorphous powders.

[0279] Compound 11:

[0280] .sup.1H NMR (300 MHz, DMSO-d.sub.6) δ ppm 3.56-3.69 (m, 5H) 3.75-3.90 (m, 2H) 3.95 (s, 3H) 4.79 (t, J=5.5 Hz, 1H) 5.73 (s, 1H) 5.95 (d, J=1.9 Hz, 2H) 6.18 (d, J=8.3 Hz, 1H) 6.41 (d, J=8.3 Hz, 1H) 6.97 (dd, J=8.1, 1.7 Hz, 1H) 7.10 (d, J=1.9 Hz, 1H) 7.16-7.30 (m, 1H) 7.36 (d, J=8.3 Hz, 1H) 7.92 (dd, J=8.7, 4.5 Hz, 1H) 8.50 (s, 1H) 12.78 (br. s., 1H)

[0281] LC/MS (method LC-D): R.sub.t 1.52 min, MH.sup.+ 517

[0282] Enantiomer 11A:

[0283] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 12.79 (br. s., 1H) 8.50 (s, 1H) 7.92 (dd, J=8.7, 4.3 Hz, 1H) 7.36 (d, J=8.5 Hz, 1H) 7.20-7.27 (m, 1H) 7.10 (d, J=1.6 Hz, 1H) 6.97 (dd, J=8.4, 1.7 Hz, 1H) 6.42 (d, J=8.2 Hz, 1H) 6.18 (d, J=8.2 Hz, 1H) 5.95 (d, J=1.6 Hz, 2H) 5.72 (s, 1H) 4.79 (t, J=5.4 Hz, 1H) 3.94 (s, 3H) 3.78-3.89 (m, 2H) 3.59-3.68 (m, 5H)

[0284] LC/MS (method LC-C): R.sub.t 3.07 min, MH.sup.+ 517

[0285] [α].sub.D.sup.20: −99.6° (c 0.2218, DMF)

[0286] Chiral SFC (method SFC-D): R.sub.t 1.80 min, MH.sup.+ 517, chiral purity 100%.

[0287] Enantiomer 11B:

[0288] .sup.1H NMR (500 MHz, DMSO-d.sub.6) δ ppm 12.79 (br. s., 1H) 8.50 (s, 1H) 7.91 (dd, J=8.7, 4.3 Hz, 1H) 7.36 (d, J=8.2 Hz, 1H) 7.17-7.28 (m, 1H) 7.10 (d, J=1.9 Hz, 1H) 6.97 (dd, J=8.2, 1.9 Hz, 1H) 6.41 (d, J=8.2 Hz, 1H) 6.18 (d, J=8.2 Hz, 1H) 5.95 (d, J=1.9 Hz, 2H) 5.72 (t, J=1.9 Hz, 1H) 4.79 (t, J=5.5 Hz, 1H) 3.94 (s, 3H) 3.77-3.88 (m, 2H) 3.55-3.70 (m, 5H)

[0289] LC/MS (method LC-C): R.sub.t 3.07 min, MH.sup.+ 517

[0290] [α].sub.D.sup.20: +99.2° (c 0.2127, DMF)

[0291] Chiral SFC (method SFC-D): R.sub.t 3.38 min, MH.sup.+ 517, chiral purity 100%.

[0292] Antiviral Activity of the Compounds of the Invention

[0293] DENV-2 Antiviral Assay

[0294] The antiviral activity of all the compounds of the invention was tested against DENV-2 16681 strain which was labeled with enhanced green fluorescent protein (eGPF). The culture medium consists of minimal essential medium supplemented with 2% of heat-inactivated fetal calf serum, 0.04% gentamycin (50 mg/mL) and 2 mM of L-glutamine. Vero cells, obtained from ECACC, were suspended in culture medium and 25 μL was added to 384-well plates (2500 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 4-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (200 nL). In addition, each compound concentration is tested in quadruplicate (final concentration range: 25 μM-0.00038 μM). Finally, each plate contains wells which are assigned as virus controls (containing cells and virus in the absence of compound), cell controls (containing cells in the absence of virus and compound) and medium controls (containing medium in the absence of cells, virus and compounds). To the wells assigned as medium control, 25 μL of culture medium was added instead of Vero cells. Once the cells were added to the plates, the plates were incubated for 30 minutes at room temperature to allow the cells to distribute evenly within the wells. Next, the plates were incubated in a fully humidified incubator (37° C., 5% CO.sub.2) until the next day. Then, DENV-2 strain 16681, labeled with eGFP, was added at a multiplicity of infection (MOI) of 0.5. Therefore, 15 μL of virus suspension was added to all the wells containing test compound and to the wells assigned as virus control. In parallel, 15 μL of culture medium was added to the medium and cell controls. Next, the plates were incubated for 3 days in a fully humidified incubator (37° C., 5% CO.sub.2). At the day of the read out, the eGFP fluorescence was measured using an automated fluorescence microscope at 488 nm (blue laser). Using an in-house LIMS system, inhibition dose response curves for each compound were calculated and the half maximal effective concentration (EC.sub.50) was determined. Therefore, the percent inhibition (I) for every test concentration is calculated using the following formula: I=100*(S.sub.T−S.sub.CC)/(S.sub.VC−S.sub.CC); S.sub.T, S.sub.CC and S.sub.VC are the amount of eGFP signal in the test compound, cell control and virus control wells, respectively. The EC.sub.50 represents the concentration of a compound at which the virus replication is inhibited with 50%, as measured by a 50% reduction of the eGFP fluorescent intensity compared to the virus control. The EC.sub.50 is calculated using linear interpolation (Table 1).

[0295] In parallel, the toxicity of the compounds was assessed on the same plates. Once the read-out for the eGFP signal was done, 10 μL of resazurin, a cell viability stain, was added to all wells of the 384-well plates. The resazurin assay is based on the reduction of the blue resazurin by NADH, produced by the cells, into the highly fluorescent product, resorufin. The formation of pink fluorescent resorufin is directly related to the number of viable cells in the well. The plates were incubated for an additional 5 hours in a fully humidified incubator (37° C., 5% CO.sub.2). Next, the plates were measured on an Infinite reader (Tecan) using an excitation wavelength of 530 nm. The half maximal cytotoxic concentration (CC50) was also determined, defined as the concentration required to reduce the resazurin conversion by 50% compared to that of the cell control wells (Table 1). Finally, the selectivity index (SI) is determined for the compounds, which is calculated as followed: SI=CC.sub.50/EC.sub.50.

TABLE-US-00004 TABLE 1 EC.sub.50, CC.sub.50, and SI for the compounds of the invention in the DENV-2 antiviral assay EC50 CC50 compound# (μM) N (μM) N SI N 1.sup.  0.0030 4 6.2 4 2053 4 1A 0.00081 14 4.3 16 5395 13 1B 0.14 7 6.3 7 44 7 2A 0.00081 4 4.2 4 5166 4 2B 0.081 3 6.2 3 77 3 3.sup.  0.00072 3 5.5 3 7587 3 3A 0.00034 4 4.5 4 20070 4 3B 0.045 3 7.1 3 158 3 4.sup.  0.0014 3 6.3 4 4398 3 4A 0.00070 3 4.9 3 6965 3 4B 0.045 3 7.4 3 164 3 5.sup.  0.0023 3 6.5 3 2860 3 5A 0.00084 4 5.9 4 7057 4 5B 0.40 3 8.1 3 21 3 6.sup.  0.0012 3 5.6 3 4639 3 6A 0.00082 15 5.1 15 6520 14 6B 0.076 4 5.4 4 71 4 7.sup.  0.0026 3 7.4 3 2860 3 7A 0.0011 4 6.1 4 5569 4 7B 0.069 3 7.8 3 112 3 8.sup.  0.0024 3 8.0 3 3270 3 8A 0.0012 3 4.2 3 3457 3 8B 0.26 3 5.7 3 22 3 9.sup.  0.0022 3 7.3 3 3329 3 9A 0.0044 4 3.9 4 899 4 9A 0.076 3 6.5 3 85 3 10 .sup.  0.0075 3 5.8 3 767 3 10A  0.0032 3 4.5 3 1416 3 10B  0.19 4 3.8 3 20 3 11 .sup.  0.0031 3 5.8 3 1879 3 11A  0.090 3 6.5 3 72 3 11B  0.0012 3 4.1 3 3526 3 N = the number of independent experiments in which the compounds were tested.

[0296] Tetravalent Reverse Transcriptase Quantitative-PCR (RT-qPCR) Assay: Protocol A.

[0297] The antiviral activity of the compounds of the invention was tested against DENV-1 strain TC974#666 (NCPV), DENV-2 strain 16681, DENV-3 strain H87 (NCPV) and DENV-4 strains H241 (NCPV) and EDEN (SG/06K2270DK1/2005; GenBank accession number QG398256) in a RT-qPCR assay. Therefore, Vero cells were infected with either DENV-1, or -2, or -3, or -4 in the presence or absence of test compounds. At day 3 post-infection, the cells were lysed and cell lysates were used to prepare cDNA of both a viral target (the 3′UTR of DENV; table 2) and a cellular reference gene (β-actin, table 2). Subsequently, a duplex real time PCR was performed on a Lightcycler480 instrument. The generated Cp value is inversely proportional to the amount of RNA expression of these targets. Inhibition of DENV replication by a test compound result in a shift of Cp's for the 3′UTR gene. On the other hand, if a test compound is toxic to the cells, a similar effect on β-actin expression will be observed. The comparative ΔΔCp method is used to calculate EC.sub.50, which is based on the relative gene expression of the target gene (3′UTR) normalized with the cellular housekeeping gene (β-actin). In addition, CC.sub.50 values were determined based on the Cp values acquired for the housekeeping gene β-actin.

TABLE-US-00005 TABLE 2 Primers and probes used for the real-time, quantitative RT-PCR. Primer/probe Target Sequence.sup.a,b F3utr258 DENV 3′-UTR 5′-CGGTTAGAGGAGACCCCTC-3′ R3utr425 DENV 3′-UTR 5′-GAGACAGCAGGATCTCTGGTC-3′ P3utr343 DENV 3′-UTR AAGGACTAGAGGTTAGAGGAGACCCCCC-3′- Factin743 β-actin 5′-GGCCAGGTCATCACCATT-3′ Ractin876 β-actin 5′-ATGTCCACGTCACACTTCATG-3′ Pactin773 β-actin TTCCGCTGCCCTGAGGCTCTC-3′-BHQ1 .sup.aReporter dyes (FAM, HEX) and quencher (BHQ1) elements are indicated in bold and italics. .sup.bThe nucleotide sequence of the primers and probes were selected from the conserved region in the 3′UTR region of the dengue virus genome, based on the alignment of 300 nucleotide sequences of the four dengue serotypes deposited in Genbank (Gong et al., 2013, Methods Mol Biol, Chapter 16).

[0298] The culture medium consisted of minimal essential medium supplemented with 2% of heat-inactivated fetal calf serum, 0.04% gentamycin (50 mg/mL) and 2 mM of L-glutamine. Vero cells, obtained from ECACC, were suspended in culture medium and 75 μL/well was added in 96-well plates (10000 cells/well), which already contain the antiviral compounds. Typically, these plates contain a 4-fold serial dilution of 9 dilution steps of the test compound at 200 times the final concentration in 100% DMSO (500 nL; final concentration range: 25 μM-0.00038 μM). In addition, each plate contains wells which are assigned as virus controls (containing cells and virus in the absence of compound) and cell controls (containing cells in the absence of virus and compound). Once the cells were added in the plates, the plates were incubated in a fully humidified incubator (37° C., 5% CO.sub.2) until the next day. Then, DENV-1 strain TC974#666, DENV-2 strain 16681, DENV-3 strain H87 or DENV-4 strains H241 or EDEN were added. Therefore, 25 μL of virus suspension, where a Cp of ˜22 was achieved in RTqPCR, was added to all the wells containing test compound and to the wells assigned as virus control. In parallel, 25 μL of culture medium was added to the cell controls. Next, the plates were incubated for 3 days in a fully humidified incubator (37° C., 5% CO.sub.2). After 3 days, the supernatant was removed from the wells and the cells were washed twice with ice-cold PBS (˜100 μL). The cell pellets within the 96-well plates were stored at −80° C. for at least 1 day. Next, RNA was extracted using the Cells-to-CT™ lysis kit, according to the manufacture's guideline (Applied Biosystems). The cell lysates can be stored at −80° C. or immediately used in the reverse transcription step.

[0299] In preparation of the reverse transcription step, mix A (table 3A) was prepared and 7.57 μL/well was dispensed in a 96-well plate. After addition of 5 μL of the cell lysates, a five minute denaturation step at 75° C. was performed (table 3B). Afterwards, 7.43 μL of mix B was added (table 3C) and the reverse transcription step was initiated (table 3D) to generate cDNA.

[0300] Finally, a RT-qPCR mix was prepared, mix C (table 4A), dispensed in 96-well LightCycler qPCR plates to which 3 μL of cDNA was added and the qPCR was performed according to the conditions in table 4B on a LightCycler 480.

[0301] Using the LightCycler software and an in-house LIMS system, dose response curves for each compound were calculated and the half maximal effective concentration (EC.sub.50) and the half maximal cytotoxic concentration (CO.sub.50) were determined (Tables 6-9).

TABLE-US-00006 TABLE 3 cDNA synthesis using Mix A, denaturation, Mix B and reverse transcription. A Mix A Plates 8 Samples 828 Reaction Vol. (μl) 20 Concentration Volume for (μl) Mix Item Unit Stock Final 1 sample x samples Milli-Q H.sub.2O 7.27 6019.56 R3utr425 μM 20 0.27 0.15 124.20 Ractin876 μM 20 0.27 0.15 124.20 Volume 7.57 mix/well (μl) Cell lysates 5.00 B Denaturation step: Step Temp Time Denaturation 75° C. 5′ Hold  4° C. hold C Mix B Samples 864 Concentration Volume for (μl) Mix Item Unit Stock Final 1 sample x samples Expand HIFI X 10.00 1.00 2.00 1728.0 buffer 2 MgCl.sub.2 mM 25.00 3.50 2.80 2419.2 dNTPs mM 10.00 1.00 2.00 1728.0 Rnase inhibitor U/μl 40.00 1.00 0.50 432.0 Expand RT U/μl 50.00 0.33 0.13 112.3 Total Volume 7.43 Mix (μl) D Protocol cDNA synthesis Step Temp Time Rev transc 42° C. 30′ Denaturation 99° C.  5′ Hold  4° C. hold

TABLE-US-00007 TABLE 4 qPCR mix and protocol. A Mix C Samples 833 Reaction Vol. (μl) 25 Concentration Volume for (μl) Mix Item Unit Stock Final 1 sample x samples H.sub.2O PCR grade 7.74 6447.42 Roche Roche 2xMM mix X 2 1 12.50 10412.50 F3utr258 μM 20 0.3 0.38 316.54 R3utr425 μM 20 0.3 0.38 316.54 P3utr343 μM 20 0.1 0.13 108.29 Factin743 μM 20 0.3 0.38 316.54 Ractin876 μM 20 0.3 0.38 316.54 Pactin773 μM 20 0.1 0.13 108.29 Volume Mix/ 22.02 Tube (μl) cDNA 3.00 B Protocol qPCR3 Ramp Step Temp Time rate preincub/denat 95° C. 10 min 4.4 Denaturation 95° C. 10 sec 4.4 annealing 58° C.  1 min 2.2 40 cycles Elongation 72° C.  1 sec 4.4 Cooling 40° C. 10 sec 1.5

[0302] Tetravalent Quantitative Reverse Transcriptase-PCR (RT-qPCR) Assay: Protocol B.

[0303] Vero cells (4×10.sup.4) were seeded in 96-well plates. After one day, cell culture medium was replaced with 100 μL assay medium containing a 2×, 3× or 5× serial dilution of the compound (concentration range: 50 μg/mL-0.00038 μg/mL, 50 μg/mL-0.0076 μg/mL, and 50 μg/mL-0.00013 μg/mL, respectively) and 100 μL of dengue virus dilution, where a Ct of ˜20 is achieved in RTqPCR. Following a 2 hour incubation period, the cell monolayer was washed 3 times with assay medium to remove residual, non-adsorbed virus and cultures were further incubated for either 4 days (DENV-2 NGC-Tongalike) or 7 days (DENV-1 Djibouti strain D1/H/IMTSSA/98/606, DENV-3 strain H87 prototype, DENV-4 strain H241) in the presence of the inhibitor. Supernatant was harvested and viral RNA load was determined by real-time quantitative RT-PCR. The 50% effective concentration (EC.sub.K), which is defined as the compound concentration that is required to inhibit viral RNA replication by 50%, was determined using logarithmic interpolation (Tables 7 and 8).

[0304] RNA was isolated from 100 μL supernatant with the NucleoSpin 96 Virus kit (Filter Service, Duren, Germany) as described by the manufacturer. The sequences of the TaqMan primers (DENV-For, DENV-Rev; Table 5) and TaqMan probes (DENV-Probe Table 5) were selected from non-structural gene 3 (NS3) or NSS, of the respective flaviviruses using Primer Express software (version 2.0; Applied Biosystems, Lennik, Belgium). The TaqMan probe was fluorescently labelled with 6-carboxyfluorescein (FAM) at the 5′ end as the reporter dye, and with minor groove binder (MGB) at the 3′ end as the quencher (Table 5). One-step, quantitative RT-PCR was performed in a total volume of 25 μL, containing 13.9375 μL H.sub.2O, 6.25 μL master mix (Eurogentec, Seraing, Belgium), 0.375 μL forward primer, 0.375 μL reverse primer, 1 μL probe, 0.0625 μL reverse transcriptase (Eurogentec) and 3 μL sample. RT-PCR was performed using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Branchburg, N.J., USA) using the following conditions: 30 min at 48° C. and 10 min at 95° C., followed by 40 cycles of 15 s at 95° C. and 1 min at 60° C. The data was analyzed using the ABI PRISM 7500 SDS software (version 1.3.1; Applied Biosystems). For absolute quantification, standard curves were generated using 10-fold dilutions of template preparations of known concentrations.

TABLE-US-00008 TABLE 5 Primers and probes used for real-time, quantitative RT-PCR. Primer/Probe Sequence (5′ .fwdarw. 3′).sup.a Source.sup.b Target DENV-For TCGGAGCCGGAGTTTACAAA DENV2 NS3 (SEQ ID N. 1) NGC DENV-Rev TCTTAACGTCCGCCCATGAT (SEQ ID N. 2) DENV-Probe ATTCCACACAATGTGGCAT- (SEQ ID N. 3) DenS GGATAGACCAGAGATCCTGCTGT DENV-1, −3, −4 NS5 (SEQ ID N. 4) DenAS1-3 CATTCCATTTTCTGGCGTTC DENV-1, −3 (SEQ ID N. 5) DenAS4 CAATCCATCTTGCGGCGCTC DENV-4 (SEQ ID N. 6) DEN_1-3 probe CAGCATCATTCCAGGCACAG- DENV-1, −3 (SEQ ID N. 7) DEN_4 probe CAACATCAATCCAGGCACAG- DENV-4 (SEQ ID N. 8) .sup.aReporter dye (FAM) and quencher (MGB/TAMRA) elements are indicated in bold and italics. .sup.bThe nucleotide sequence and position of the primers and probes within the genome were deduced from the nucleotide sequence of DENV 2 NGC (GenBank accession no. M29095; Irie et al., 1989), dengue virus serotype 1 Djibouti strain D1/H/IMTSSA/98/606 (Genbank Accession Number AF298808), dengue virus serotype 3 strain H87 prototype (c93130), dengue virus serotype 4 strain H241 (no sequences available).

[0305] Cytotoxic Assay (Protocol B)

[0306] Potential cytotoxic effects of the compounds were evaluated in uninfected Vero cells. Cells were seeded at 4×10.sup.4 cells/well in a 96-well plate in the presence of two-, three- or five-fold serial dilutions (ranging from 50 μg/mL-0.0038 μg/mL, 50 μg/mL-0.0076 μg/mL, and 50 μg/mL-0.00013 μg/mL, respectively) of compound and incubated for 4 to 7 days. Culture medium was discarded and 100 μL 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium/phenazinemethosulfate (MTS/PMS; Promega, Leiden, The Netherlands) in PBS was added to each well. Following a 2-hour incubation period at 37° C., the optical density was determined at 498 nm. Cytotoxic activity was calculated using the following formula: % cell viability=100×(OD.sub.Compound/OD.sub.CC), where OD.sub.Compound and OD.sub.CC correspond to the optical density at 498 nm of the uninfected cell cultures treated with compound and that of uninfected, untreated cell cultures, respectively. The 50% cytotoxic concentration (i.e., the concentration that reduces the total cell number with 50%; CC.sub.50) was calculated using linear interpolation (Tables 7 and 8).

TABLE-US-00009 TABLE 6 EC.sub.50, CC.sub.50, and SI for the compounds against serotype 1 in the RT-qPCR assays Protocol A RT-qPCR serotype 1 TC974#666 EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.0077 5 4.1 5 530 5 2A 0.0071 4 4.5 4 628 4 3A 0.0053 3 3.8 3 721 3 4A 0.0078 4 4.6 3 447 3 5A 0.013 3 5.2 3 392 3 6A 0.0036 5 4.8 5 1348 5 7A 0.016 3 4.6 3 282 3 8A 0.0096 3 3.5 3 368 3 9A 0.0050 3 4.7 3 934 3 10A  0.014 3 4.1 3 290 3 11B  0.018 3 3.7 3 202 3 Protocol B RT-qPCR serotype 1 Djibouti EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.022 3 9.7 9 506 2 2A 0.017 2 7.4 5 447 2 3A 0.018 2 7.7 5 450 2 4A 0.020 2 9.5 5 392 2 5A 0.019 2 7.8 5 471 2 6A <0.015 3 8.1 7 >693 3 7A 0.020 2 7.2 5 366 2 8A 0.017 2 7.7 5 450 2 9A 0.019 2 7.9 5 454 2 10A  0.020 2 8.0 5 413 2 11B  0.025 2 7.7 5 320 2 N = the number of independent experiments in which the compounds were tested.

TABLE-US-00010 TABLE 7 EC.sub.50, CC.sub.50, and SI for the compounds against serotype 2 in the RT-qPCR assays Protocol A RT-qPCR serotype 2 16681 EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.0011 9 4.7 9 4457 9 2A 0.00085 4 5.7 4 6744 4 3A 0.00024 3 4.3 6 25632 3 4A 0.00044 5 5.2 6 12915 5 5A 0.00050 4 4.5 5 10575 4 6A 0.00054 7 3.7 7 8391 6 7A 0.00091 3 4.7 4 5763 3 8A 0.0012 3 5.2 3 4406 3 9A 0.00079 3 3.8 3 4833 3 10A  0.0032 3 4.2 3 1312 3 11B  0.0010 3 4.7 3 4562 3 Protocol B RT-qPCR serotype 2 NGC-Tongalike EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.00038 5 14 6 36900 5 2A 0.00040 3 13 3 31100 3 3A <0.00033 3 12 3 >37200 3 4A <0.00034 3 13 3 >37300 3 5A <0.00040 3 11 3 >28000 3 6A 0.00040 4 13 4 29300 3 7A 0.00027 3 12 2 18900 2 8A 0.0014 3 11 3 8050 3 9A <0.00031 3 12 3 >37300 3 10A  0.0014 3 12 3 8910 3 11B  0.00027 3 11 3 37900 1 N = the number of independent experiments in which the compounds were tested.

TABLE-US-00011 TABLE 8 EC.sub.50, CC.sub.50, and SI for the compounds against serotype 3 in the RT-qPCR assays Protocol A RT-qPCR serotype 3 H87 EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.063 5 1.7 4 28 4 2A 0.053 4 3.9 3 76 3 3A 0.036 3 3.7 3 104 3 4A 0.065 4 3.8 2 52 2 5A 0.068 3 2.9 3 42 3 6A 0.026 5 3.0 4 106 4 7A 0.070 3 4.6 2 66 2 8A 0.076 3 3.6 3 47 3 9A 0.063 3 3.0 3 48 3 10A  0.070 3 3.3 3 47 3 11B  0.070 3 3.4 3 48 3 Protocol B RT-qPCR serotype 3 H87 EC50 CC50 compound# (μM) N (μM) N SI N 1A <0.015 6 9.7 9 >1440 6 2A 0.021 3 7.4 5 350 3 3A 0.020 3 7.7 5 383 3 4A 0.029 3 9.5 5 376 3 5A 0.025 3 7.8 5 291 3 6A <0.015 4 8.1 7 >591 4 7A <0.017 3 7.2 5 >423 3 8A 0.032 3 7.7 5 244 3 9A 0.018 3 7.9 5 400 3 10A  0.027 3 8.0 5 291 3 11B  0.026 3 7.7 5 294 3 N = the number of independent experiments in which the compounds were tested.

TABLE-US-00012 TABLE 9 EC.sub.50, CC.sub.50, and SI for the compounds against serotype 4 strains H241 (A) and SG/06K2270DK1/2005 (B) in the RT-qPCR assays Protocol A RT-qPCR serotype 4 H241 EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.24 10 3.1 9 13 9 2A 0.26 4 1.7 3 7 3 3A 0.18 5 3.0 5 17 5 4A 0.24 4 2.4 4 10 4 5A 0.32 4 4.5 4 13 4 6A 0.084 8 2.1 8 25 8 7A 0.23 3 2.8 3 12 3 8A 0.30 3 3.1 2 11 2 9A 0.24 3 2.7 3 11 3 10A  0.32 3 2.7 2 8 2 11B  0.35 3 2.5 3 7 3 Protocol A RT-qPCR serotype 4 EDEN EC50 CC50 compound# (μM) N (μM) N SI N 1A 0.0062 6 3.7 6 583 6 2A 0.0043 5 4.4 5 1007 5 3A 0.0029 4 4.1 3 1579 3 4A 0.0052 5 4.4 3 981 3 5A 0.0046 3 4.2 2 630 2 6A 0.0029 3 3.6 3 1241 3 7A 0.0066 4 3.7 3 536 3 8A 0.0065 4 4.5 4 690 4 9A 0.0047 3 2.6 3 717 3 10A  0.0086 4 4.4 3 436 3 11B  0.0098 4 4.0 3 374 3 N = the number of independent experiments in which the compounds were tested.