COMPOSITION ESSENTIALLY FREE OF WATER AND COMPRISING AT LEAST ONE SPORE FORMING FUNGAL BIOLOGICAL CONTROL AGENT, A POLYETHER-MODIFIED TRISILOXANE AND FUMED OR PRECIPITATED SILICA

Abstract

The present invention relates to a liquid composition comprising at least one biological control agent wherein said biological control agent is spores of a spore forming fungus, a polyether-modified trisiloxane and fumed silica or precipitated silica, wherein said composition is essentially free of water, said composition being useful in plant protection.

Claims

1. A liquid composition comprising at least one biological control agent, wherein said biological control agent is spores of a spore forming fungus, a polyether-modified trisiloxane and fumed silica or precipitated silica, wherein said composition is essentially free of water.

2. The composition according to claim 1, which is essentially free of oil.

3. The composition according to claim 1 further comprising an antifoaming agent.

4. The composition according to claim 1, wherein the concentration of said fumed silica or precipitated silica is in the range of 0.1 to 9 wt.-%.

5. The composition of claim 1, wherein said biological control agent is a fungus that exhibits activity against insects, acarids, spiders, nematodes, molluscs, bacteria, rodents, weeds and/or phytopathogens.

6. The composition according to claim 1, claim 1 further comprising at least one further plant protective agent.

7. The composition according to claim 1, claim 1, wherein said biological control agent is present in the form of conidia or chlamydospores.

8. The composition according to claim 1, wherein the spores of said spore forming fungus are dried spores.

9. The composition of claim 1, wherein said spores are present in a concentration of between about 1×10.sup.5/ml and 2×10.sup.11/ml.

10. The composition according to claim 1, wherein said biological control agent has fungicidal activity and is selected from Coniothyrium minitans; Microsphaeropsis ochracea Trichoderma spp.; Arthrobotrys dactyloides; Arthrobotrys oligospora; Arthrobotrys superba; Aspergillus flavus; Aspergillus flavus; Gliocladium roseum; Phlebiopsis (or Phlebia or Peniophora) gigantea; Phlebiopsis (or Phlebia or Peniophora) gigantea, Pythium oligandrum; Talaromyces flavus; Ampeloymces quisqualis; Chaetomium cupreum; Chaetomium globosum; Cladosporium cladosporioides; Dactylaria candida; Dilophosphora alopecuri; Fusarium oxysporum; Gliocladium catenlatum; Lecanicillium lecanii (formerly known as Verticillium lecanii) conidia; Penicillium vermiculatum; Ulocladium oudemanii; Verticillium albo-atrum (formerly V. dahliae); Verticillium chlamydosporium.

11. The composition according to claim 1, wherein said biological control agent has insecticidal activity and is selected from Beauveria bassiana strain ATCC 74040; Beauveria bassiana strain GHA (Accession No. ATCC74250); Beauveria bassiana strain ATP02 (Accession No. DSM 24665); Beauveria bassiana strain CG 716; Hirsutella citriformis; Hirsutella thompsonii; Lecanicillium lecanii (formerly known as Verticillium lecanii) conidia of strain KV01; Lecanicillium lecanii (formerly known as Verticillium lecanii) conidia of strain DAOM198499; Lecanicillium lecanii (formerly known as Verticillium lecanii) conidia of strain strain DAOM216596; Lecanicillium muscarium (formerly Verticillium lecanii) strain VE 6/CABI(=IMI) 268317/CBS102071/ARSEF5128; Metarhizium anisopliae strain F52 (DSM3884/ATCC 90448); M. anisopliae var acridum; M. anisopliae var acridum isolate IMI 330189; Nomuraea rileyi; Paecilomyces fumosoroseus (new: Isaria fumosorosea) strain apopka 97; Paecilomyces fumosoroseus (new: Isaria fumosorosea) strain FE 9901; Aschersonia aleyrodis; Beauveria brongniartii; Metarhizium flavoviride; Mucor haemelis.

12. The composition according to claim 1, wherein said biolocal control agent has nematicidal activity and is selected from Paecilomyces lilacinus strain 251; Trichoderma koningii; Harpasporium anguillullae; Hirsutella minnesotensis; Monacrosporum cionopagum; Monacrosporium psychrophilum; Myrothecium verrucaria strain AARC-0255; Stagonospora phaseoli; Trichoderma lignorum strain TL-0601; Fusarium solani strain Fs5; Hirsutella rhossiliensis; Monacrosporium drechsteri; Monacrosporium gephyropagum; Nematoctonus geogenius; Nematoctonus leiosporus; Pochonia chlamydosporia (also known as Vercillium chlamydasporium) var. catenulata IMI SD 187, CU); Stagonospora heteroderae; Meristacrum asterospermum and Duddingtonia flagrans.

13. The composition according to claim 1, wherein said biological control agent which supports and/or promotes and/or stimulates plant health and plant growth and is Penicillium bilaii.

14. The composition according to claim 1, wherein said biological control agent is spores of Paecilomyces lilacinus.

15. The composition according to claim 14, wherein said Paecilomyces lilacinus is Paecilomyces lilacinus strain 251 or a mutant thereof.

16. The composition according to claim 1, wherein the amount of polyether-modified trisiloxane ranges between 50 and 96% wt.

17. Method of producing a composition according to claim 1, comprising the steps of providing a carrier comprising a polyether-modified trisiloxane and fumed silica or precipitated silica in a concentration that in the final composition results in a concentration of between 50 and 96% wt polyether-modified trisiloxane and between about 0.1 and 9% wt fumed silica or precipitated silica, and incorporating a biological control agent into said carrier liquid, wherein said biological control agent is spores of a spore forming fungus.

18. Method for controlling phytopathogenic fungi, insects, spiders, molluscs, weeds, rodents and/or nematodes in a plant, for enhancing growth of a plant or for increasing plant yield or root health comprising applying the composition of any one of claims 1 to 16 to said plant or to a plot where plants are to be grown.

19. (canceled)

20. (canceled)

21. The composition according to claim 10, wherein said biological control agent is selected from Coniothyrium minitans strain CON/M/91-8 (Accession No. DSM-9660); Microsphaeropsis ochracea strain P130A (ATCC deposit 74412) Trichoderma atroviride, strain SC1; Trichoderma harzianum rifai strain KRL-AG2; Aspergillus flavus, strain NRRL 21882; Aspergillus flavus, strain AF36; Gliocladium roseum, strain 321U; B2.15 Phlebiopsis (or Phlebia or Peniophora) gigantea strain VRA 1835 (ATCC 90304); Phlebiopsis (or Phlebia or Peniophora) gigantea strain VRA 1984 (DSM16201); Phlebiopsis (or Phlebia or Peniophora) gigantea strain VRA 1985 (DSM16202); Phlebiopsis (or Phlebia or Peniophora) gigantea strain VRA 1986 (DSM16203); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG B20/5 (IMI390096); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG SP log6 (IMI390097); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG SP log5 (IMI390098); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG BU3 (IMI390099); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG BU4 (IMI390100); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG 410.3 (IMI3901.01); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG 97/1062/116/1.1 (IMI390102); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG B22/SP1287/3.1 (IMI390103); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG SH1 (IMI390104); Phlebiopsis (or Phlebia or Peniophora) gigantea strain FOC PG B22/SP1190/3.2 (IMI390105); Pythium oligandrum strain DV74 or M1 (ATCC 38472); Talaromyces flavus strain VII7b; Trichoderma asperellum strain ICC 012; Trichoderma asperellum strain SKT-1; Trichoderma atroviride strain CNCM 1-1237; Trichoderma atroviride strain no. V08/002387; Trichoderma atroviride strain NMI no. V08/002388; Trichoderma atroviride strain NMI no. V08/002389; Trichoderma atroviride strain NMI no. V08/002390; Trichoderma atroviride strain LC52; Trichoderma atroviride strain ATCC 20476 (IMI 206040); Trichoderma atroviride strain T11 (IMI352941/CECT20498); Trichoderma harmatum; Trichoderma harzianum; Trichoderma harzianum rifai T39; Trichoderma harzianum strain KD; Trichoderma harzianum strain ITEM 908; Trichoderma harzianum strain TH35; Trichoderma virens (also known as Gliocladium virens strain GL-21; Trichoderma viride, strain TV1; Ampelomyces quisqualis strain AQ 10; Cladosporium cladosporioides strain H39; Dactylaria candida; Dilophosphora alopecuri; Fusarium oxysporum strain Fo47; Gliocladium catenulatum (Synonym: Clonostachys rosea f. catenulate) strain J1446; Lecanicillium lecanii (formerly known as Verticillium lecanii) conidia of strain KV01; Trichoderma atroviride strain SKT-1 (FERM P-16510); Trichoderma atroviride strain SKT-2 (FERM P-16511); Trichoderma atroviride strain SKT-3 (FERM P-17021); Trichoderma gamsii (formerly T. viride) strain ICC080; Trichoderma harzianum strain DB 103; Trichoderma polysporum strain IMI 206039; Trichoderma stromaticum; Ulocladium oudemansii strain HRU3; Verticillium albo-atrum (formerly V. dahliae) strain WCS850; and mixtures of Trichoderma asperellum strain ICC 012 and Trichoderma gamsii strain ICC 080 (product known as e.g., BIO-TAM™ from Bayer CropScience LP, US).

22. The composition according to claim 13, wherein said Penicillium bilaii is Penicillium bilaii strain ATCC 22348 or a mutant thereof.

Description

EXAMPLE 1

Synthesis of Polyether-Modified Siloxane Surfactant within the Scope of the Invention

[0106] Firstly, 198 g of a polyether of the general formula

CH.sub.2═CHCH.sub.2O[CH.sub.2CH.sub.2O].sub.12Me

were placed in a 500 ml three-necked flask with stirrer and reflux condenser and heated to 90° C. 10 ppm of Pt in form of the Karstedt catalyst were added and the mixture was stirred for 10 minutes. After this 52 g of 1,1,1,3,5,5,5-Heptamethyltrisiloxane were added dropwise within 15 minutes under constant stirring. An exothermic reaction was observed. Finally, the mixture was stirred for 4 h at 90° C.

EXAMPLE 2

Evaluation of Sedimentation Rate of Compositions Comprising Mixtures of a Polyether-Modified Trisiloxane and Fumed Silica and Conidia of Purpureocillium Lilacinum

[0107] A test was carried out using a pure polyether-modified trisiloxane as negative control and 3 mixtures of polyether-modified trisiloxane with fumed silica. To create the mixtures the following ratios of polyether-modified trisiloxane (BreakThru S 240) and fumed silica (Aerosil 200) have been used: 97.5:2.5, 95.0:5.0, 92.5:7.5. Aerosil 200 was mixed into BreakThru S 240 applying an Ultra Turax for 10 min at 10,000 rpm and for further 5 min at 5600 rpm. The four liquids then where mixed with Purpureocillium lilacinum conidia powder in a way that 20.25 g conidia powder (about 7×10.sup.12 spores/g) were blended into 99.75 grams each liquid using an Ultra Turrax for 2 min at 5400 rpm. The resulting conidia suspensions contained 5.57×10.sup.10 viable conidia per gram. The conidia suspensions then were filled into 20 ml glass bottles up to 1.5 cm below the top sealed and incubated at 54° C. to evaluate the influence of the mixtures on conidia sedimentation.

[0108] The sedimentation of the conidia on the bottom of the flasks where evaluated after 31 days by determining the height of the clear supernatant on top of the dark brown conidia suspension/sediment in percent of the complete height (see table 1 below).

TABLE-US-00001 TABLE 1 Height of the clear supernatant on top of the conidia sediment after incubation of different conidia formulation at 54° C. using a pure polyether-modified trisiloxane or mixtures of polyether-modified trisiloxane with fumed silica in different ratios. Height of the supernatant at Incubation ratio of BreakThru S 240 and time Aerosil 200 in the test mixture (in %) at 54° C. 100:0 97.5:2.5 95.0:5.0 92.5:7.5 31 days 78.1 68.8 0.0 0.0

[0109] The results clearly indicate that, for Purpureocillium lilacinum conidia, a content of fumed silica of about 5% or higher homogenously mixed into a polyether-modified trisiloxane prevents the conidia from settling down.

EXAMPLE 3

Viability of Conidia in Formulations Comprising Polyether-Modified Trisiloxane and Fumed Silica as Compared to One Only Comprising Polyether-Modified Trisiloxane

[0110] Four liquids comprising polyether-modified trisiloxane without (negative control) and with different amounts of fumed silica were prepared as described in Example 1, filled into 20 ml glass bottles up to 1.5 cm below the top, sealed and incubated at 30° C. to evaluate the influence of the mixtures on the conidia viability (see table 2 below).

TABLE-US-00002 TABLE 2 Viability of the conidia of Purpureocillium lilacinum in BreakThru S 240 and BreakThru S 240 blended with Aerosil 200 at 30° C. Conidia viability in % after incubation at 30° C. after Used liquid Ratio of 2 4 6 8 10 12 16 21 conidia carrier blending weeks weeks weeks weeks weeks weeks weeks weeks BreakThru S 240 n.a. 83.30 84.23 77.78 78.99 76.90 78.64 64.62 50.62 BreakThru S 240 97.5:2.0 86.30 86.32 81.25 81.14 80.28 76.02 74.44 66.78 blended with Aerosil 200 BreakThru S 240 95.0:5.0 88.31 86.88 85.43 84.84 80.69 78.93 62.32 55.78 blended with Aerosil 200 BreakThru S 240 92.5:7.5 90.61 88.61 84.60 82.89 81.22 80.68 71.87 47.83 blended with Aerosil 200

[0111] The viability of the conidia has not been significantly negatively affected by the addition of the fumed silica Aerosil 200.

EXAMPLE 4

Detailed Evaluation of Sedimentation Rate of Compositions Comprising Mixtures of a Polyether-Modified Trisiloxane and Fumed Silica and Conidia of Purpureocillium Lilacinum

[0112] A test was carried out using a pure polyether-modified trisiloxane as negative control and 7 mixtures of polyether-modified trisiloxane with fumed silica. To create the mixtures the following ratios of polyether-modified trisiloxane (BreakThru S 240) and fumed silica (Aerosil 200) have been used: 93.5:6.5, 94.0:6.0, 94.5:5.5, 95.0:5.0, 95.5:4.5, 96.0:4.0. Aerosil 200 was mixed into the BreakThru S 240 applying an Ultra Turax for 10 min at 10,000 rpm and for further 5 min at 5600 rpm. The 8 resulting liquids then where mixed with Purpureocillium lilacinum conidia powder in a way that 20.23 g (containing about 7×10.sup.12 spores/g) conidia powder were blended into 99.77 grams of each liquid using an Ultra Turrax for 2 min at 5400 rpm. The resulting conidia suspensions contained 5.56×10.sup.10 viable conidia per gram. The conidia suspensions then were filled into 20 ml glass bottles up to 1.5 cm below the top, sealed and incubated at 30 and 54° C. to evaluate the influence of the mixtures on conidia sedimentation.

[0113] Sedimentation of the conidia on the bottom of the flasks where evaluated after 14 and 30 days by determination of the height of the clear supernatant on top of the dark brown conidia suspension/sediment in percent of the complete height (see tables 3 and 4 below).

TABLE-US-00003 TABLE 3 Height of the clear supernatant on top of the conidia sediment after incubation of different conidia formulation at 54° C. using a pure polyether-modified trisiloxane or mixtures of polyether-modified trisiloxane with fumed silica in different ratios. Incubation time at Height of the supernatant at ratio of BreakThru S 240 and Aerosil 200 in the test mixture (in %) 54° C. 100:0 96.5:3.5 96.0:4.0 95.5:4.5 95.0:5.0 94.5:5.5 94.0:6.0 96.5:3.5 14 days 40.6 18.8 9.4 4.7 1.6 0.0 0.0 0.0 30 days 71.9 31.3 14.1 6.3 1.6 0.0 0.0 0.0

TABLE-US-00004 TABLE 4 Height of the clear supernatant on top of the conidia sediment after incubation of different conidia formulation at 30° C. using a pure polyether-modified trisiloxane or mixtures of polyether-modified trisiloxane with fumed silica in different ratios. Incubation time at Height of the supernatant at ratio of BreakThru S 240 and Aerosil 200 in the test mixture (in %) 30° C. 100:0 96.5:3.5 96.0:4.0 95.5:4.5 95.0:5.0 94.5:5.5 94.0:6.0 96.5:3.5 14 days 18.8 9.4 4.7 3.1 1.6 0.6 0.0 0.0 30 days 28.1 9.4 5.3 4.7 2.2 0.6 0.0 0.0

[0114] The results clearly indicate that, for conidia of Purpureocillium lilacinum, a content of fumed silica of about 5% or higher homogenously mixed into a polyether-modified trisiloxane prevents the conidia from settling down.

EXAMPLE 5

More Detailed Evaluation of Viability of Conidia in Formulations Comprising Polyether-Modified Trisiloxane and Fumed Silica as Compared to One Only Comprising Polyether-Modified Trisiloxane

[0115] Eight liquids comprising polyether-modified trisiloxane without (negative control) and with different amounts of fumed silica were prepared as described in Example 3, filled into 20 ml glass bottles up to 1.5 cm below the top, sealed and incubated at 30° C. to evaluate the influence of the mixtures on conidia viability (see table 5 below).

TABLE-US-00005 TABLE 5 Viability of the conidia of Purpureocillium lilacinum in BreakThru S 240 and BreakThru S 240 blended with Aerosil 200 at 30° C. Conidia viability in % after incubation at 30° C. after Used liquid Ratio of 1 2 4 6 8 10 12 17 conidia carrier blending weeks weeks weeks weeks weeks weeks weeks weeks BreakThru S 240 n.a. 94.63 82.68 83.15 71.36 69.28 69.69 61.43 63.05 BreakThru S 240 96.5:3.5 94.35 83.70 84.31 81.58 71.80 70.65 68.34 32.55* blended with Aerosil 200 BreakThru S 240 96.0:4.0 93.43 83.57 85.84 76.70 70.98 69.06 64.39 62.84 blended with Aerosil 200 BreakThru S 240 95.5:4.5 93.92 83.22 86.74 78.08 71.88 69.60 64.44 50.56 blended with Aerosil 200 BreakThru S 240 95.0:5.0 93.26 83.51 84.18 79.40 69.69 72.22 68.57 34.11* blended with Aerosil 200 BreakThru S 240 94.5:5.5 93.41 84.46 85.34 77.23 71.96 70.65 72.62 33.33* blended with Aerosil 200 BreakThru S 240 94.0:6.0 94.84 84.69 86.27 77.00 72.52 69.29 71.06 35.00* blended with Aerosil 200 BreakThru S 240 96.5:3.5 94.28 84.16 85.11 75.95 71.53 72.35 70.58 53.90 blended with Aerosil 200 *most likely experimental error, due to surplus of oxygen

[0116] The viability of the conidia has not been negatively affected by the addition of the fumed silica Aerosil 200.

EXAMPLE 6

Evaluation whether or not Dried Conidia of Cladosporium Cladosporioides Suspended in a Liquid Comprising a Mixture of a 95% Polyether-Modified Trisiloxane and and 6% Fumed Silica are Undergoing Sedimentation with Time

[0117] A test was carried out using a mixture of polyether-modified trisiloxane with fumed silica. To create the mixtures a fumed silica (Aerosil 200) was mixed into a polyether-modified trisiloxane (BreakThru S 240) at a ratio of 6:94 applying an Ultra Turax for 10 min at 10,000 rpm and for further 5 min at 5600 rpm. The resulting liquid then was mixed with Cladosporium cladosporioides conidia powder in a way that 16 g conidia powder (about 8×10.sup.11 conidia) were blended into 100 grams of the liquid using an Ultra Turax for 2 min at 5400 rpm. The resulting conidia suspensions contained 5.5×10.sup.9 viable conidia per gram. The conidia suspensions then were filled into 20 ml glass bottles up to 1.5 cm below the top, sealed and incubated for at 50° C. to evaluate the influence of the mixtures on conidia sedimentation.

[0118] After an incubation time of 30 days no sedimentation of the conidia could be observed. There was no clear supernatant on top of the dark grey conidia suspension/sediment detectable.

EXAMPLE 7

Efficacy of the Composition according to the Invention Comprising Spores of Paecilomyces Lilacinus in Comparison with a WP Formulation of Paecilomyces Lilacinus

[0119] Efficacy against root knot nematodes (Meloidogyne incognita) of a WG formulation dispersed in water and the formulation according to the invention both comprising equivalent spore concentrations of Paecilomyces lilacinus was compared in bell pepper, tomato, cucumber and lettuce at different locations. Applications were done either by drip or drench application which does not have an influence on efficacy. At the specified time (days after first application or days after last application), root health was evaluated.

[0120] The results are depicted in the table below:

[0121] Bell Pepper

TABLE-US-00006 Amount applied (amount of Time of Efficacy No. of formulation spores) measurement (ABBOT) applications BioAct WG  2 kg/ha 127 d after first 13.6 4 application BioAct DC 400 ml/ha* 127 d after first 31.8 4 application *equivalent to about 2.2 × 10.sup.13 spores per hectare

[0122] Cucumber

TABLE-US-00007 Trial 1 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 42 d after first 37.1 4 application BioAct DC 800 ml/ha 42 d after first 40.2 4 application

TABLE-US-00008 Trial 2 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 14/34 d after last 66.7/51.0 2 application BioAct DC 800 ml/ha 14/34 d after last 72.0/75.4 2 application

TABLE-US-00009 Trial 3 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 63 d after first 44.2 3 application BioAct DC 800 ml/ha 63 d after first 47.5 3 application

[0123] Tomato

TABLE-US-00010 Trial 1 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 56/145 d after first 54.0/27.2 4 application BioAct DC 800 ml/ha 56/145 d after first 49.3/29.1 4 application

TABLE-US-00011 Trial 2 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 71/140 d after first 35.2/34.9 4 application BioAct DC 800 ml/ha 71/140 d after first 34.1/37.7 4 application

[0124] Lettuce

TABLE-US-00012 Amount Time of Efficacy No. of formulation applied measurement (ABBOT) applications BioAct WG  4 kg/ha 51 d after first 54.8 3 application BioAct DC 800 ml/ha 51 d after first 76.2 3 application

EXAMPLE 8

Moisture Content in the Spore Powder Used in the Formulation according to the Invention

[0125] To create the mixtures a fumed silica (Aerosil 200) was mixed into a polyether-modified trisiloxane (BreakThru S 240) at a ratio of 6:94 applying an Ultra Turax for 10 min at 10,000 rpm and for further 5 min at 5600 rpm.

[0126] The resulting liquid then was mixed with Purpureocillium lilacinum conidia powder in a way that 18.9 g (moisture content of 9%) or 18.3 g (moisture content of 6%) conidia powder (about 3.3×10.sup.11 spores/g) were blended into 81.1 grams or 81.7 grams using an Ultra Turrax for 2 min at 5400 rpm. The resulting conidia suspensions contained 5.50×10.sup.10 viable conidia per gram. Another sample with spores of the fungus Isaria fumosorosea was prepared in that 41.9 g conidia powder (about 1.6×10.sup.11 spores/g) were blended into 58.1 grams each liquid using an Ultra Turrax for 2 min at 5400 rpm. The resulting conidia suspension contained 5.50×10.sup.10 viable conidia per gram. The conidia suspensions then were filled into 20 ml glass bottles up to 1.5 cm below the top sealed and incubated at 30° C. to evaluate the storage stability of the conidia.

[0127] Viability was measured at the time points indicated in tables 6 and 7 below.

TABLE-US-00013 TABLE 6 Purpureocillium lilacinum Time point Viability % Moisture content of spore powder % 9 6 initiation 97 98 4 weeks 83.6 92.8

TABLE-US-00014 TABLE 7 Isaria fumosorosea Time point Viability % Moisture content of spore powder % 9 initiation 89.6 2 weeks 67.8