331 NEW FOUND PROTEINS IN PEANUT WITH THEIR SEQUENCE AND THE METHOD OF PRODUCING SUPPLEMENT INCLUDING BEVERAGES ON THE BASIS OF JUICE AND POWDER FROM THE PEANUT

Abstract

331 new found proteins in peanut with their sequence and the method of producing supplement including beverages on the basis of juice and powder from the peanut. 490 new found proteins in cashew sequence and method of producing food supplements including beverages on the basis of juice and powder from the cashew. 843 new found proteins in edamame (young soy) sequence and method of producing food supplement including beverages on the basis of juice and powder from the edamame (young soy)

Claims

1. A food supplement composition arranged for human consumption comprising a plurality of ingredients, all from peanut. The invention provides a process for obtaining juice and powder from peanut, wherein the seed of peanut is grinded and centrifuged to make juice and powder. Total 837 proteins found in peanut. Among them, 296 are uncharacterized and 35 are putative uncharacterized new found KH good healthy proteins synthesized by KH good healthy cells.

2. Method of claim 1, wherein juice is mixed beverage that will contain certain number of cells

3. Method of claim 2, wherein Powder is mixed food supplement that will also contain certain number of cells

4. Method of claim 2, peanut has KH GOOD HEALTHY CELLS in which the RNA synthesizes good proteins that: 1—Send signal to the DAMAGED, SICK, AND BAD CELLS that triggers that synthesis of good proteins that transform these cells to become GOOD healthy cells. 2—Send signal to the other currently undamaged cells to synthesis of good proteins to protect them from being DAMAGED, INFECTED and PRONE to DNA and other cellular alterations. 3—Send signal to the body to produce new cells that are healthy and forbid them from being affected by intra- and extracellular damaging signals.

5. Method of claim 2, in peanut contains proteins whose molecular weight are similar to the molecular weight of the human proteins of human albumin, immunoglobulin and APOA-1.

6. Method of claim 2, living cells are found in this dried powder and beverages

7. A food composition arranged for human consumption comprising a plurality of ingredients, all from cashew. The invention provides a process for obtaining food supplement of juice and powder from cashew, wherein the seed of cashew is grinded and centrifuged to make juice and powder. Total 682 proteins found in cashew, among them, 490 are uncharacterized new found KH good healthy proteins synthesized by KH good healthy cells.

8. Method of claim 6, wherein juice is mixed beverage that will contain certain number of cells

9. Method of claim 7, wherein Powder is mixed food supplement that will also contain certain number of cells

10. Method of claim 7, cashew has KH GOOD HEALTHY CELLS in which the RNA synthesizes good proteins that: 1—Send signal to the DAMAGED, SICK, AND BAD CELLS that triggers that synthesis of good proteins that transform these cells to become GOOD healthy cells. 2—Send signal to the other currently undamaged cells to synthesis of good proteins to protect them from being DAMAGED, INFECTED and PRONE to DNA and other cellular alterations. 3—Send signal to the body to produce new cells that are healthy and forbid them from being affected by intra- and extracellular damaging signals.

11. Method of claim 7, in cashew contains proteins whose molecular weights are similar to the molecular weight of the human proteins of human albumin, immunoglobulin and APOA-1.

12. Method of claim 7, living cells are found in this dried powder and beverages

13. A food supplement composition arranged for human consumption comprising a plurality of ingredients, all from edamame. The invention provides a process for obtaining juice and powder from edamame, wherein the seed of edamame is grinded and centrifuged to make juice and powder. Total 1,223 proteins found in edamame, among them, 679 are uncharacterized and 164 are putative uncharacterized new found KH good healthy proteins synthesized by KH good healthy cells.

14. Method of claim 13, wherein juice is mixed beverage that will contain certain number of cells

15. Method of claim 14, wherein Powder is mixed food supplement that will also contain certain number of cells

16. Method of claim 14, edamame has KH GOOD HEALTHY CELLS in which the RNA synthesizes good proteins that: 1—Send signal to the DAMAGED, SICK, AND BAD CELLS that triggers that synthesis of good proteins that transform these cells to become GOOD healthy cells. 2—Send signal to the other currently undamaged cells to synthesis of good proteins to protect them from being DAMAGED, INFECTED and PRONE to DNA and other cellular alterations. 3—Send signal to the body to produce new cells that are healthy and forbid them from being affected by intra- and extracellular damaging signals.

17. Method of claim 14, in edamame contains proteins whose molecular weight are similar to the molecular weight of the human proteins of human albumin, immunoglobulin and APOA-1.

18. Method of claim 14, living cells are found in this dried powder and beverages

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0005] FIG. 1 flow chart of peanut process

[0006] FIG. 2 SDS-Page of supernatant of KH 169 Peanut

[0007] FIG. 3 SDS-Page of paste of KH 169 Peanut

[0008] FIG. 4 flow chart of cashew process

[0009] FIG. 5 SDS-Page of supernatant of KH 170 cashew

[0010] FIG. 6 SDS-Page of paste of KH 170 cashew

[0011] FIG. 7 flow chart of edamame process

[0012] FIG. 8 SDS-Page of supernatant of KH 169 Edamame

[0013] FIG. 9 SDS-Page of paste of KH 169 Edamame

[0014] Table 1 cell count in the supernatant and paste of KH 169 peanut

[0015] Table 2 cell count in the supernatant and paste of KH 170 cashew

[0016] Table 3 cell count in the supernatant and paste of KH 169 edamame

DETAILED DESCRIPTION OF THE INVENTION

Peanut

[0017] The present invention describes a method for the production of a food supplement including juice and powder from peanut. [0018] The peanut is treated immediately after harvest. [0019] Wash the peanut in cleaning bath at 5 about 0-100 Celsius. [0020] Smash the whole peanut in grinder to cut the thick stem at about 0-100 Celsius. Collect all the raw materials. [0021] Grind the raw materials in superfine mill at about 0-100 Celsius. Collect the raw juice. [0022] Check the pH of the raw juice, about 1.5-10 [0023] Centrifuge the raw juice at low to high rpm with normal centrifuge at about −30 to 30 Celsius. Collect the precipitate (“ppt”) and supernatant. [0024] Add 2-4 volume water to ppt. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 Celsius to 60. Collect the ppt 15 and supernatant. [0025] Add 1-2 volume water to it. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 to 60 Celsius. Collect the ppt and supernatant. [0026] Mix all the supernatant for 10-100 minutes at about 0-100 Celsius. [0027] Check the supernatant pH, about 1.5 to 10 [0028] Centrifuge the supernatant juice at moderate to high rpm with a disc centrifuge at about −30 to 60 Celsius, collect the juice. [0029] Check the juice pH, about. 2.5 to 10 pH

[0030] 1. If making juice

[0031] Homogenizing the juice at 40-60 MPa.

[0032] Sterilize the juice at 130-140 Celsius for 5-20 seconds with a pipe sterilizer.

[0033] Bottle the clear juice at 25-35 Celsius. Take sample and check the pH

[0034] 2. If making powder

[0035] The juice is concentrated in Single-effect Falling Film Evaporator at 65-85 Celsius until the brix value is 30-50.

[0036] The concentrated juice is transfer to the Centrifugal Spray Dryer to get powder.

[0037] 3. Mix all the 5 ppt to make capsules, tablet or powder in pouch.

TABLE-US-00004 TABLE 1 cell count in the supernatant and paste of KH 169 peanut Supernatant (/mL) S1 S2 S3 S4 S5 KH 169 Peanut 7,690,000 2,110,000 226,000 5,540,000 5,380,000 Paste (/mL) P1 P2 P3 P4 P5 KH 169 Peanut 659,000,000 1,324,000,000 1,320,000,000 439,000,000 116,000,000

Cashew

[0038] The present invention describes a method for the production food supplement of juice and powder from cashew. [0039] The cashew is treated immediately after harvest. [0040] Wash the cashew in cleaning bath at 5 about 0-100 Celsius. [0041] Smash the whole cashew in grinder to cut the thick stem at about 0-100 Celsius. Collect all the raw materials. [0042] Grind the raw materials in superfine mill at about 0-100 Celsius. Collect the raw juice. [0043] Check the pH of the raw juice, about 1.5-10 [0044] Centrifuge the raw juice at low to high rpm with normal centrifuge at about −30 to 30 Celsius. Collect the precipitate (“ppt”) and supernatant. [0045] Add 2-4 volume water to ppt. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 Celsius to 60. Collect the ppt 15 and supernatant. [0046] Add 1-2 volume water to it. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 to 60 Celsius. Collect the ppt and supernatant. [0047] Mix all the supernatant for 10-100 minutes at about 0-100 Celsius. [0048] Check the supernatant pH, about 1.5 to 10 [0049] Centrifuge the supernatant juice at moderate to high rpm with a disc centrifuge at about −30 to 60 Celsius, collect the juice. [0050] Check the juice pH, about 1.5 to 10 pH

[0051] 1. If making juice:

[0052] Homogenizing the juice at 40-60 MPa.

[0053] Sterilize the juice at 130-140 Celsius for 5-20 seconds with a pipe sterilizer.

[0054] Bottle the clear juice at 25-35 Celsius. Take sample and check the pH

[0055] 2. If making powder:

[0056] The juice is concentrated in Single-effect Falling Film Evaporator at 65-85 Celsius until the brix value is 30-50.

[0057] The concentrated juice is transfer to the Centrifugal Spray Dryer to get powder.

[0058] Packaging the powder.

[0059] 3. Mix all the 5 ppt to make tablets, capsules or powder in pouch.

TABLE-US-00005 TABLE 2 cell count in the supernatant and paste of KH 170 cashew Supernatant (/mL) S1 S2 S3 S4 S5 KH 170 cashew 5,760,000 3,690,000 5,310,000 11,200,000 2,950,000 Paste (/mL) P1 P2 P3 P4 P5 KH 170 cashew 231,000,000 246,000,000 94,900,000 346,000,000 276,000,000

Edamame

[0060] The present invention describes a method for the production of food supplement including juice and powder from edamame (young soy). [0061] The edamame is treated immediately after harvest. [0062] Wash the edamame in cleaning bath at 5 minutes about 0-100 Celsius. [0063] Smash the whole edamame in grinder to cut the thick stem at about 0-100 Celsius. Collect all the raw materials. [0064] Grind the raw materials in superfine mill at about 0-100 Celsius. Collect the raw juice. [0065] Check the pH of the raw juice, about 1.5-10 [0066] Centrifuge the raw juice at low to high rpm with normal centrifuge at about −30 to 30 Celsius. Collect the precipitate (“ppt”) and supernatant. [0067] Add 2-4 volume water to ppt. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 Celsius to 60. Collect the ppt 1 and supernatant. [0068] Add 1-2 volume water to it. Mix about 10-100 minutes and centrifuge again at low to high rpm with normal centrifuge at about −30 to 60 Celsius. Collect the ppt and supernatant. [0069] Mix all the supernatant for 10-100 minutes at about 0-100 Celsius. [0070] Check the supernatant pH, about 1.5 to 10 [0071] Centrifuge the supernatant juice at moderate to high rpm with a disc centrifuge at about −30 to 60 Celsius, collect the juice. [0072] Check the juice pH, about 1.5 to 10 pH

[0073] 1. If making juice

[0074] Homogenizing the juice at 40-60 MPa.

[0075] Sterilize the juice at 130-140 Celsius for 5-20 seconds with a pipe sterilizer.

[0076] Bottle the clear juice at 25-35 Celsius. Take sample and check the pH

[0077] 2. If making powder

[0078] The juice is concentrated in Single-effect Falling Film Evaporator at 65-85 Celsius until the brix value is 30-50.

[0079] The concentrated juice is transfer to the Centrifugal Spray Dryer to get powder.

[0080] Packaging the powder.

[0081] 3. Mix all the 5 ppt to make capsules, tablets or powder in pouch.

TABLE-US-00006 TABLE 3 cell count in the supernatant and paste of KH 169 edamame Supernatant (/mL) S1 S2 S3 S4 S5 KH 111 7,690,000 2,110,000 226,000 5,540,000 5,380,000 Edamame Paste (/mL) P1 P2 P3 P4 P5 KH 111 659,000,000 1,324,000,000 1,320,000,000 439,000,000 116,000,000 Edamame