MULTIPLEXER FOR CONTROLLING FLUID IN MICROFLUIDICS CHIP AND MICROFLUIDICS CHIP ASSEMBLY

Abstract

A multiplexer for controlling a fluid in a microchannel by controlling pneumatic pressure in the microchannel in a microfluidics chip includes: a first pneumatic channel; and a second pneumatic channel forming a cross point which is in communication with the first pneumatic channel, wherein the cross point is in communication with the microchannel of the microfluidics chip, and predetermined pneumatic pressure is provided to the microchannel by using a combination of providing of the pneumatic pressure to the first and second pneumatic channels, channel closing, or channel opening.

Claims

1. A multiplexer for controlling a fluid in a microchannel by controlling pneumatic pressure in the microchannel in a microfluidics chip, the multiplexer comprising: a first pneumatic channel; and a second pneumatic channel forming a cross point which is in communication with the first pneumatic channel, wherein the cross point is in communication with the microchannel of the microfluidics chip, and predetermined pneumatic pressure is provided to the microchannel by using a combination of pneumatic pressing, channel closing, or channel opening to the first and second pneumatic channels.

2. The multiplexer of claim 1, wherein the pneumatic pressure is provided to the microchannel, when the pneumatic pressure is provided to both the first and second pneumatic channels or when the pneumatic pressure is provided to any one side of the first and second pneumatic channels and the other side is closed.

3. The multiplexer of claim 1, wherein two or more of M first pneuamtic channels separated from each other are provided, two or more of N second pneumatic channels separated from each other are provided, each of the second pneumatic channels including branch channels branched to correspond to the number M of first pneumatic channels, and the branch channel forms the cross point by crossing the first pneumatic channel.

4. The multiplexer of claim 3, wherein the pneumatic pressure may be controlled to be provided independently to M*N microchannels by using M+N first and second pneumatic channels.

5. The multiplexer of claim 1, further comprising: a first lamination plate having the first pneumatic channel and a second lamination plate having the second pneumatic channel, wherein while the second lamination plate overlaps with the first lamination plate, the second pneumatic channel crosses the first pneumatic channel to form the cross point to be in communication with each other, and while the microfluidics chip vertically overlaps with the first and second lamination plates, the microchannel is in communication with the cross point.

6. The multiplexer of claim 5, wherein the first lamination plate, the second lamination plate, and the microfluidics chip are sequentially laminated from the top to the bottom, and the first pneumatic channel, the second pneumatic channel, and the microchannel are provided to the first lamination plate, the second lamination plate, and the microfluidics chip, respectively in a groove shape and the second pneumatic channel being provided in a hole shape at the cross point.

7. The multiplexer of claim 6, wherein a first through-hole for providing the pneumatic pressure to the first pneumatic channel and a second through-hole for providing the pneumatic pressure to the second pneumatic channel of the second lamination plate are formed in the first laminate plate.

8. A microfluidics chip assembly for controlling a fluid in a microchannel with pneumatic pressure, the microfluidics chip assembly comprising: a first lamination plate having a first pneumatic channel; and a second lamination plate having a second pneumatic channel and a cross point where the second pneumatic channel crosses the first pneumatic channel to form the cross point to be in communication with each other; and a microfluidics chip including a microchannel which is in communication with the cross point while vertically overlapping with the first and second lamination plates, wherein predetermined pneumatic pressure is provided to the microchannel by using a combination of pneumatic pressing, channel closing, or channel opening to the first and second pneumatic channels.

9. The microfluidics chip assembly of claim 8, wherein two or more of M first pneumatic channels separated from each other are provided, two or more of N second pneumatic channels separated from each other are provided, each of the second pneumatic channels including branch channels branched to correspond to the number M of first pneumatic channels, and the branch channel forms the cross point by crossing the first pneumatic channel and the microchannel is in communication with the cross point.

10. The microfluidics chip assembly of claim 8, wherein the first lamination plate, the second lamination plate, and the microfluidics chip are sequentially laminated from the top to the bottom, and the first pneumatic channel, the second pneumatic channel, and the microchannel are provided to the first lamination plate, the second lamination plate, and the microfluidics chip, respectively in a groove shape and the second pneumatic channel is provided in a hole shape at the cross point.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG. 1 is a perspective view of a microfluidics chip assembly according to an exemplary embodiment of the present invention.

[0023] FIG. 2 is an exploded perspective view of the microfluidics chip assembly. FIG. 3 is a schematic structural diagram of a pneumatic channel and a microchannel for describing that air is selectively injected into the microchannel of a microfluidics chip by using a multiplexer.

[0024] FIG. 4 illustrates a microfluidics chip assembly capable of controlling (M*N) microchannels by using (M+N) pneumatic channels according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0025] Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited or restricted to the exemplary embodiments. For reference, in the description, like reference numerals substantially refer to like elements, which may be described by citing contents disclosed in other drawings under such a rule and contents determined to be apparent to those skilled in the art or repeated may be omitted.

[0026] FIG. 1 is a perspective view of a microfluidics chip assembly according to an exemplary embodiment of the present invention. FIG. 2 is an exploded perspective view of the microfluidics chip assembly and FIG. 3 is a schematic structural diagram of a pneumatic channel and a microchannel for describing that air is selectively injected into the microchannel of a microfluidics chip by using a multiplexer.

[0027] Referring to FIGS. 1 to 3, the microfluidics chip assembly 100 according to the exemplary embodiment includes a multiplexer 130 and a microfluidics chip 140.

[0028] The multiplexer 130 is formed by laminating two lamination plates and microchannels 141 are formed in the microfluidics chip 140 provided in a plate type like the lamination plate.

[0029] The plates are preferably made of glass or silicones and synthetic materials which less react with acid or base or other biochemical materials. In particular, polymethylsiloxane may be used and the materials as polymer materials which may be bonded to each other may implement a stable tight contact state between the plates and although described later in detail, plates having the pneumatic channels or the microchannels provided a shape of a groove dug on the surface of the plate with a predetermined depth, such as a groove or a hole penetrating the plate tightly contact each other to effectively maintain a sealing state of the channels.

[0030] First, the multiplexer 130 has a first lamination plate 110 and a second lamination plate 120 and a first pneumatic channel 111 is formed on the first lamination plate 110. The first pneumatic channel 111 is provided in the groove shape formed on the surface of the first lamination plate 110. In addition, a second pneumatic channel 121 is formed on the second lamination plate 120 and the second pneumatic channel 121 may also be provided in the groove shape formed on the surface of the second lamination plate 120. However, the first and second pneumatic channel 111 and 121 need to be in communication with each other at a cross point 123 and a part of the second pneumatic channel 121 may be thus provided in the hole shape vertically penetrating the second lamination plate 120 at the cross point 123.

[0031] The first and second lamination plates 110 and 120 of the multiplexer 130 are laid on the microfluidics chip 140 and during this process, the microchannels 141 formed in the microfluidics chip 140 may be in communication with the cross point 124 formed by the first and second pneumatic channel 111 and 121.

[0032] As seen in FIGS. 1 to 3, in the case of a lamination order, the first lamination plate 1110, the second lamination plate 120, and the microfluidics chip 140 are sequentially laminated from the top to the bottom and a second through-hole 114 for providing a pneumatic pressure to the second pneumatic channel 121 of the second lamination plate 120 disposed below the first lamination plate 110 penetrates the first lamination plate 110 and similarly, a first through-hole 112 for providing the pneumatic pressure to the first pneumatic channel 111 is formed.

[0033] Through the multiplexer 130, the pneumatic pressure may be provided to the microchannels 141 of the microfluidics chip 140 disposed to tightly contact the bottom of the multiplexer 130. The pneumatic pressure is provided to the microchannels 141 of the microfluidics chip 140 to serve as a pneumatic valve and perform even basic function to analyze a profile in which a small quantity of materials to be analyzed flows to the microchannels to reach with various biomolecules or sensors aggregated in the chip.

[0034] In particular, the first pneumatic channel 111 and the second pneumatic channel 121 of the multiplexer 130 of the present invention are in communication with each other at the cross point 123 and due to such a reason, when the pneumatic pressure is provided to any one side, the pneumatic pressure leaks to the other side. This is illustrated in detail in FIG. 3 and in detail, the case is illustrated in FIG. 3C. Of course, a predetermined amount of pneumatic pressure may flow in the microchannels 141, but this may be appreciated that a reagent or a specimen in the microchannels 141 or only very little pneumatic pressure not to serve as the pneumatic valve is transferred.

[0035] That is, it is easy to transfer pneumatic pressure at a designed level to the microchannels 141 only when the pneumatic pressure is provided to both the first and second pneumatic channels 111 and 121 as illustrated in FIG. 3A. For reference, in FIG. 3B, illustrated is a case where the pneumatic pressure is provided to only any one side of the first and second pneumatic channels 111 and 121, but the other side is closed, and as a result, the pneumatic pressure is provided to the microchannels 141.

[0036] Meanwhile, air provided to the pneumatic channels is controlled through the solenoid valve and the air is provided to the pneumatic channels or the aforementioned closing state is implemented by operating an on/off state of the solenoid valve. For reference, the air is preferably nitrogen which is inert gas, but a type of the air is appropriately selected by the reagent or specimen and is not limited to the nitrogen and the valve may also be replaced with another device which may selectively provide/interrupt external gas to the pneumatic channels and is not limited only to the aforementioned solenoid valve.

[0037] Meanwhile, in the related art, the solenoid valves need to be disposed in all microchannels one to one in order to provide the pneumatic pressure to the microchannels, and as a result, it is difficult to miniaturize a facility and a high-density screening test itself is difficult.

[0038] However, the solenoid valves need not be disposed in the microchannels one to one by using the multiplexer 130 according to the present invention and manufacturing cost may be reduced and the miniaturization of the facility may be implemented.

[0039] Referring to FIGS. 1 or 2, the multiplexer 130 has the first lamination plate 110 having three first pneumatic channels 111 and the second lamination plate 120 having two second pneumatic channels 121.

[0040] Further, the second pneumatic channels 121 is branched into a plurality of branch channels 122 and the number of branch channels 122 is provided to correspond to the number of first pneumatic channels 111. Herein, the “correspond” means that when the number of branch channels 122 is larger than the number of first pneumatic channels 111, branch channels not connected to the first pneumatic channels are present, and as a result, the number of branch channels included in one second pneumatic channel may be provided to be equal to or less than the total number of first pneumatic channels.

[0041] In the exemplary embodiment, the number of branch channels 122 included in each second pneumatic channel 121 is three similarly to the number of first pneumatic channels 111. Accordingly, only 5 solenoid valves which is the sum total of 3 which is the number of first pneumatic channels 111 and 2 which is the number of second pneumatic channels 121 are connected to the first and second through-holes 112 and 114 to selectively provide the pneumatic pressure and may control each of 6 branch channels 122 which is a multiplication of the number of first pneumatic channels 111 and the number of second pneumatic channels 121 and individually control the microchannels 141 connected to the branch channels 122.

[0042] When the exemplary embodiment is generalized, as illustrated in FIG. 4, two or more of M first pneumatic channels 111 and N second pneumatic channels 121 separated from each other are provided and each second pneumatic channel 121 includes branch channels 122 branched to correspond to the number M of first pneumatic channels 111 and the branch channel 122 forms the cross point with the first pneumatic channel 111. In this case, the multiplexer 130 may be provided, which may control at least M*N microchannels 141 by using M+N first and second pneumatic channels 111 and 121. In detail, when it is assumed that each second pneumatic channel 121 includes branch channels 122 of the same number as the first pneumatic channels 111, the pneumatic pressure may be provided independently to the respective M*N microchannels 141 by using M+N first and second pneumatic channels 111 and 121.

[0043] The present invention has been described with reference to the preferred embodiments of the present application. However, it will be appreciated by those skilled in the art that various modifications and changes of the present invention can be made without departing from the spirit and the scope of the present invention which are defined in the appended claims and their equivalents.