Pesticide residue detection data platform based on high resolution mass spectrum, internet and data science, and method for automatically generating detection report

Abstract

Disclosed is a pesticide residue detection data platform based on high resolution mass spectrum, the Internet and data science, and a method for automatically generating a detection report. The platform includes allied laboratories, a detection result database of the allied laboratories, four basic sub-databases, a data collection system and an intelligent data analysis system. The intelligent analysis system reads data according to conditions set by a user, performs various statistical analyses according to a statistical analysis model, generates charts, obtains a comprehensive conclusion, and returns an analysis result to the client ends of the allied laboratories.

Claims

1. A computerized, network-enabled pesticide residue detection data platform receiving data inputs based on high-resolution mass spectrometry, Internet, and data science including laboratory coordination, a coordinated laboratory detection result database and four basic sub-databases, a data acquisition system, and an intelligent data analysis system, wherein, the coordinated laboratory includes a plurality of individual coordinated standard laboratories established across the country, which are operated under five uniform specifications in a closed system and detect pesticide residues in fruits and vegetables on the market throughout the country all year; the coordinated laboratory detection result database is in electronic communication with the individual coordinated standard laboratories of the coordinated laboratory and contains names of pesticides, names of agricultural products, sampling times, sampling locations, detection methods, and detection organizations; the four basic sub-databases comprise a multi-country MRL (maximum residue limit) database, an agricultural product category database, a pesticide information database, and a geographic information database, wherein the MRL database has an associated MRL query model to link pesticide residue data from the individual coordinated standard laboratories and provide a standard basis for judgment of pesticide residue detection results; the data acquisition system operated within a network-connected centralized web server, the data acquisition automatically uploading the detection result, data preprocessing, and contamination level judgment, to establish a national pesticide residue detection result database based on data transmitted from the individual coordinated standard laboratories; the data acquisition system comprising modules, wherein the data acquisition module acquires the pesticide residue detection results reported by the coordinated laboratories, processes the reported detection data, including judgment of the reported data, and supplementation, categorization, and merging of information related to pesticides, regions, and agricultural product categories, judges contamination levels according to the MRLs of different countries or regional organizations, and stores records of final results into the databases, whereby merging of the information related to pesticides, regions, and agricultural product categories further comprises merging derivatives of the pesticides, regions, and agricultural product categories reported by the coordinated laboratories to manage pesticide toxicity categorization; the intelligent data analysis system operated within the network-connected centralized web server, the intelligent data analysis system linking and communicating data between the detection result database and the four basic sub-databases, to provide multi-dimensional cross analysis of sampling locations, pesticides, agricultural products, and contamination levels according to statistical analysis models, sets and read data, and to carry out statistical analyses according to the statistical analysis models on criteria set by users of the system, to generate charts, draw comprehensive conclusions, provides detection reports, and to return data of the analytical results to users in the individual coordinated laboratories through a network connection, whereby users in the individual coordinated laboratories can view and download the data of the analytical results; the intelligent data analysis system compries modules, the intelligent data analysis system providing interface and channel of parameter set by the users, providing itemized statistics of several items, providing comprehensive analysis tasks of several items based on individual analysis results of a single item analysis, generating detection reports that contain text and charts from the analytical results, generating various statistical tables, and providing warning prompts according to the analytical results; the intelligent data analysis system includes a presentation layer, a business layer, an access layer, and a data layer; wherein the data layer consists of the detection result database, the four basic sub-databases, and relevant files, and provides database and file services; the access layer accesses the data in the databases via a database access component and to provide the data to the business layer; the business layer provides multi-dimensional statistical analysis of sampling locations, pesticides, and contamination levels according to the statistical analysis models; and the presentation layer provides various intelligent analysis reports that contain text and charts according to several criteria set by the client.

2. The pesticide residue detection data platform according to claim 1, wherein, the multi-country MRLs database includes 241,527 items of MRL standard items from China MRL, Hong Kong of China MRL, US MRL, EU MRL, Japan MRL, and Codex Alimentarius Commission (CAC) MRL standards, targeted pesticides, agricultural products, permitted MRLs, and the standard establishment countries/regions/organizations.

3. The pesticide residue detection data platform according to claim 1, wherein, the agricultural product category database comprises standards of China categorization, Hong Kong of China categorization, US categorization, EU categorization, Japan categorization, and Codex Alimentarius Commission (CAC) categorization.

4. The pesticide residue detection data platform according to claim 3, wherein, the agricultural product category database includes names of agricultural products, primary category information, secondary category information, and tertiary category information.

5. The pesticide residue detection data platform according to claim 1, wherein, the pesticide information database contains basic information, toxicity information, function information, chemical composition, prohibition information, and derivative information.

6. The pesticide residue detection data platform according to claim 5, wherein, the pesticide information database specifically comprises names of detected pesticides, CAS registry number of the pesticides, toxicity intensities of the pesticides, whether the pesticides are metabolic products and their metabolic precursors or not, and whether the pesticides are prohibited in the standards or not.

7. The pesticide residue detection data platform according to claim 1, wherein, the geographic information database covers geographical areas, and comprises detailed address of sampling locations in provincial administrative division, regional administrative division, and county-level administrative division.

8. The pesticide residue detection data platform according to claim 1, wherein, the data acquisition system is implemented on the basis of three-layer architecture consisting of browsers, a Web server, and a database server, wherein the browsers are located in the individual coordinated laboratories and are interfaces for users to access the system; the Web server is located in a data center accesses the databases and executing preprocessing logics; and the database server is located in the data center and stores and manages various pesticide residue data.

9. The pesticide residue detection data platform according to claim 1, wherein, the intelligent data analysis system is implemented on the basis of three-layer architecture consisting of browsers, a Web server, and a database server; the browsers are located in the individual coordinated laboratories throughout the country, and are interfaces for the users to access the system, set statistical parameters, and download statistical results; the Web server is also located in the data center and accesses the databases and executing various statistical analysis logics; and the database server is located in the data center and stores and manages various pesticide residue data.

10. The pesticide residue detection data platform according to claim 1, wherein, the five uniform specifications include uniform sampling, uniform sample preparation, uniform detection method, uniform format data uploading, and uniform format statistical analysis report.

11. An automatic pesticide residue detection report generation method using the pesticide residue detection data platform according to claim 1, comprising: reporting raw pesticide residue detection results to the data acquisition system from the individual coordinated laboratories distributed in the country over Internet; the data acquisition system carries out the judgment of contamination levels by data acquisition, information supplementation, derivative information merging, and toxicity analysis, and according to the MRL standards in different countries, records the results, and stores the records of results into the detection result database; the intelligent analysis system sets and reads the data according to the criteria set by the users, then performs statistical analyses of the data one by one according to statistical analysis models, generates charts, draws comprehensive conclusions, generates detection reports, and returns the analytical results to the users in the individual coordinated laboratories.

12. The automatic pesticide residue detection report generation method according to claim 11, wherein, the union laboratories detect pesticide residues by Liquid Chromatography-Quadrupole-Time of Flight/Mass Spectrometry (LC-Q-TOF/MS) and Gas Chromatography-Quadrupole-Time of Flight/Mass Spectrometry (GC-Q-TOF/MS) and report pesticide residue detection data that is detected all year.

13. The automatic pesticide residue detection report generation method according to claim 11, further comprising: setting up an online custom mode in the intelligent analysis system to support the users to select and filter the statistical data autonomously, to highlight the data of interest or key data, and to support the users to customize the type and range of report.

Description

DESCRIPTION OF DRAWINGS

(1) FIG. 1 shows the Internet pesticide residue detection data analysis platform across China;

(2) FIG. 2 shows the pesticide residue detection data acquisition system;

(3) FIG. 3 shows the intelligent pesticide residue detection data analysis system;

(4) FIG. 4 shows an automatically generated pesticide residue detection report;

(5) FIG. 5 shows a parameter selection interface for automatic export of pesticide residue detection report;

(6) FIG. 6 shows the five-level tree structure of administrative divisions for pesticide residue detection reports;

(7) FIG. 7 shows the content of a pesticide residue detection report;

(8) FIG. 8 shows the detection rates of pesticide residues in fruits and vegetables from 31 provincial capitals/municipalities markets in 2012-2015;

(9) FIG. 9 shows measurement of sample safety level according to the MRL standards of several countries, regions, or international organizations;

(10) FIG. 10 shows the toxicity categories and percentages of detected pesticides;

(11) FIG. 11 shows the species and frequencies of pesticides exceeding CAC-MRLs.

EMBODIMENTS

(12) This invention will be presented in detail with reference to the accompanying drawings and embodiments.

(13) The Internet-based national big data technical platform of pesticide residue detection is shown in FIG. 1. It comprises four main parts: {circle around (1)} more than 30 Internet-based laboratories across the country; {circle around (2)} a coordinated laboratory detection result database and four basic sub-databases (a multi-country MRLs database, an agricultural product category database, a basic information of pesticide database, and a geographic information database); {circle around (3)} a pesticide residue data acquisition system; {circle around (4)} an intelligent pesticide residue analysis system. The last two parts constitute a data processing center. The working principle of the platform is shown below. The raw pesticide residue detection results are reported from clients in the coordinated laboratories distributed in the country to the acquisition system via Internet, as shown in FIG. 2. The acquisition system carries out the judgment of the contamination levels by data acquisition, information supplementation, derivative information merging, toxicity analysis, and according to the MRL standards in different countries, records the result, and stores the records into the detection result database. The intelligent analysis system sets and reads the data according to the criteria set by the users, performs statistical data analyses one by one according to statistical analysis models, generates charts, draws general conclusions and creates detection reports. Finally, it returns the analytical results to the clients in the coordinated laboratories for viewing and downloading, as shown in FIG. 1.

(14) Table 1 shows the raw detection result database and four basic sub-databases (multi-country MRLs database, agricultural product category database, basic information of pesticide database, and geographic information database) established in more than 30 laboratories across the country. An associated data storage and query model established based on “MRL standards in several countries—categories of agricultural products—properties of more than one thousand pesticides” is proposed. Thus, linked basic pesticide residue data access and invocation is achieved, and a standard basis for judgment of the pesticide residue detection results is provided.

(15) TABLE-US-00001 TABLE 1 Five main basic databases are established to provide a scientific basis for qualitative judgment of pesticide residue detection results in coordinated laboratories Pesticide Multi-country Multi-country Geographic information MRL agricultural product information Coordinated laboratories database  criteria database  category database  database  detection result database {circle around (1)} Basic {circle around (1)} China MRLs {circle around (1)} China {circle around (1)} 7 regions {circle around (1)} Coordinated information {circle around (2)} Hong Kong of categories {circle around (2)} 34 provinces/ sampling {circle around (2)} Toxicity China MRLs {circle around (2)} Hong Kong of cities/ {circle around (2)} Coordinated information {circle around (3)} US MRLs China categories municipalities sample preparation {circle around (3)} Function {circle around (4)} EU MRLs {circle around (3)} US categories {circle around (3)} 334 {circle around (3)} Coordinated information {circle around (5)} Japan MRLs {circle around (4)} EU categories prefecture-level detection {circle around (4)} Chemical {circle around (6)} CAC MRLs {circle around (5)} Japan categories cities {circle around (4)} Coordinated component {circle around (6)} CAC categories {circle around (4)} 2,853 counties format data uploading, {circle around (5)} Prohibition {circle around (5)} Coordinated information format statistical {circle around (6)} Derivative analysis report information Pesticide 241,527 items 350 species of 2,853 counties in More than 1,200 property of MRLs agricultural China pesticides commonly categories products used in the world

(16) A pesticide residue data acquisition system is designed as shown in FIG. 2, and a national pesticide residue detection result database is established. A data integration and processing model consisting of “data acquisition—information supplementation—derivative consolidation—prohibited pesticide handling—contamination level judgment” is presented, which provides quick online acquisition and merging of pesticide residue detection result data, accurate judgment of the data according to MRLs from several countries and dynamic addition and real-time update of the pesticide residue detection result database is achieved, and provides scientific data for decision-making of food safety in the country. As shown in FIG. 2, the pesticide residue detection data acquisition system employs three-layer architecture based on browser/server. The coordinated laboratories are operated under five uniform specifications (uniform sampling, uniform sample preparation, uniform detection, uniform format data uploading, and uniform format statistical analysis report) in a closed system, utilizing Liquid Chromatography-Quadrupole-Time of Flight/Mass Spectrometry (LC-Q-TOF/MS) and Gas Chromatography-Quadrupole-Time of Flight/Mass Spectrometry (GC-Q-TOF/MS) techniques to report pesticide residue detection data that is detected in fruits and vegetables, which can fully guarantee the uniformity, integrality, accuracy, security, and reliability of data. The raw detection result data is acquired with ASP.NET technique to supply the information on pesticides, regions, and agricultural product categories merge derivatives and manage pesticide toxicity categorization. The result is judged contamination level according to the MRL criterion of the countries or regions or organizations and stored the generated records of results in the detection result database.

(17) An intelligent pesticide residue detection data analysis system is established as shown in FIG. 3. The intelligent analysis system comprises a presentation layer, a business layer, an access layer, and a data layer. The data layer consists of the detection result database, the four basic sub-databases, and relevant files, and is configured to provide database and file services. The access layer accesses the data in the databases via a database access component and provides the data to the business layer. The business layer realizes multi-dimensional statistical analysis of sampling locations, pesticides, and contamination levels according to the statistical analysis models. The presentation layer provides various intelligent analysis reports that contain text and charts according to the criterion set by a client. An online custom mode is established in the present invention to support the users to select and filter the statistical data autonomously, to highlight the data of interest or key data. Meanwhile it supports the user to customize the report type and range, to improve data presentation and big data analysis capability. It is realized that multi-dimensional automatic statistics of 20 pesticide residue indices including agricultural products, pesticides, regions, and MRLs in different countries, as shown in Table 2. Wherein, 31 different tables and 38 different figures can be generated automatically, and comprehensive assessment and warning information can be generated automatically according to the statistical results. Finally, a pesticide residue detection report that contains text and charts can be generated automatically within 30 minutes by “one-button download”, as shown in FIG. 4.

(18) FIG. 4 shows the result of multi-discipline multi-element pesticide residue big data integration technique based on ternary interdisciplinary integration technique of high-resolution mass spectrometry, Internet, and data science. “One-button download” is realized, and a detection report that contains texts and charts can be generated within 30 minutes. The pesticide residue detection report reflects 20 regular characteristics of pesticide residues in more than 150 species of fruits and vegetables in 18 categories in 31 provincial capitals/municipalities in the country, as shown in Table 2.

(19) TABLE-US-00002 TABLE 2 20 regular characteristics of pesticide residues discovered through big data statistical analysis (1) It is found that pesticide residues exist (2) Altogether 517 pesticides are detected in almost in most fruits and vegetables from 31 more than 150 species of fruits and vegetables provincial capitals/municipalities. The in 18 categories (wherein, 93 pesticides are pesticide residue detection rate is 39%-88% detected with both techniques) in China; (LC-Q-TOFMS) or 54%-97% (GC-Q-TOFMS). (3) It is found that the pass rate of pesticide (4) The normal characteristics of pesticide residues in fruits and vegetables from 31 residue detection levels (1-5, 5-10, 10-100, provincial capitals/municipalities is 100-1,000, greater than 1,000 μg/kg) in our 96.3%-98.7%, which means that the safety country are determined (by comparison with level is assured essentially; MRLs in China, EU, and Japan, etc.); (5) The normal characteristics of detected (6) The normal characteristics of detected pesticide species in individual samples (not pesticide species in the same category of found, 1 species, 2-5 species, 6-10 species, samples (not detected, 1 species, 2-5 species, more than 10 species) in our country are 6-10 species, more than 10 species) in our determined; country are determined; (7) The normal characteristics of pesticide (8) The normal characteristics of toxicity of functions (insecticides, bactericides, pesticides in our country (species of pesticides herbicides, plant growth regulators, of slightly toxic, low toxic, slightly low toxic, synergistic agents, and other species, and their moderately toxic, highly toxic, vitally toxic, proportions) in our country are determined; and prohibited, and their proportions) are determined; (9) The order of pesticide species detected (10) The order of safety (“exceeding”, throughout the country and in the provincial “detected but not exceeding”, “not detected”) capitals and the order of frequencies of of the detected pesticides throughout the detection are determined, revealing the country and in the provincial capitals is differences in pesticide application in fruits determined (by comparison with MRL and vegetables among different regions standards in China, EU, and Japan); throughout the country; (11) It is found that the MRLs in China is (12) It is found that only 40% of the massive confronted with a challenge of lower level and residue data in the general investigation is less quantity when compared with MRLs in used according to the China MRLs, while the developed countries such as USA, EU, and application ratio of the data is as high as 95% Japan; or above in EU and Japan; consequently. (13) Top 10 species of fruits and vegetables in (14) Top ten species of fruits and vegetables in which the quantities of pesticide species are which the average detected frequency of the largest and the order of follow-up fruits pesticides is the highest and the order of the and vegetables are determined; it is found that follow-up fruits and vegetables are the common fruits and vegetables are determined; contaminated severely. (15) The species of highly toxic, vitally toxic, (16) Top ten fruits and vegetables in which the and prohibited pesticides and the detection quantities of highly toxic, vitally toxic, and frequencies are determined; prohibited pesticides are the largest and the order of follow-up fruits and vegetables are determined; (17) Top ten fruits and vegetables in which the (18) The general characteristics of and the detected frequency of highly toxic, vitally differences in the existence of pesticides in the toxic, and prohibited pesticides is the highest commercial fruits and vegetables in 31 and the order of follow-up fruits and provincial capitals/municipalities are vegetables are determined; determined; (19) The characteristics of and the differences (20) The characteristics of and the differences in the pesticides detected at the sampling in the use of pesticides in 31 provincial locations in 31 provincial capitals/municipalities are determined. capitals/municipalities are determined;

(20) The download parameters of pesticide residue detection result report are shown in FIG. 5. The sampling period and type can be selected freely. One or more administrative divisions can be selected at will (a five-level architecture of “national—regional—provincial—city—county” can be achieved) as shown in FIG. 6. User can select the type of the testing instrument and export the body part or the annexed tables of the report as required. The content of the body part of a local report consists of 5 chapters, as shown in FIG. 7. The report of detection result includes various charts to help the user visually understand statistic results. For example, reflecting the detection rates of pesticide residues in fruits and vegetables from 31 provincial capitals/municipalities (see FIG. 8). Pie charts that reflect the safety levels of the detected samples, are shown in FIG. 9. Toxicity categories of detected pesticides and their proportions, are shown in FIG. 10. And bar charts that are used for out-of-specification analysis of specific samples (see FIG. 11), etc. There are 20 annexed tables which could be selected in the report. They record the raw detection results and detail statistics of concentration distribution, contamination levels, and out-of-specification (MRLs) of detected pesticide residues.

(21) A report may contain words ranging from tens of thousands of words to hundreds of thousands of words depending on the data size, and the body part and the annexed tables may contain text and charts. Such a report may be generated and downloaded by “one-button download” within 30 minutes. Thus, the analysis and reporting ability to the massive pesticide residue data is greatly improved. Besides, the automatic reporting system further supports customization and extension of report structure and content.

(22) Example of analysis report: the pesticide residue detection result database now contains 13.74 million detection data items of 22,368 batches samples of more than 140 specifies of fruits and vegetables from 638 sampling spots in 31 provincial capitals/municipalities (including 284 counties) in the country, which is stored in 10 laboratories in the country, 145 million high-resolution mass spectra are collected, and pesticide residue detection reports containing 25 million words in total are formed.

(23) The basic information of pesticide residues in fruits and vegetables from 31 provincial capitals/municipalities in the country has been investigated preliminarily, as shown in FIG. 8, Tables 3 and 4. The further general investigation of the basic situation of pesticide residues in fruits and vegetables from Beijing, Tianjin, and Hebei in 2016 is similar to that of pesticide residues in fruits and vegetables in 31 provincial capitals/municipalities in 2012-2015.

(24) TABLE-US-00003 TABLE 3 Basic information of pesticide residues in fruits and vegetables from 31 provincial capitals/municipalities (2012-2015) Item LC-Q-TOF/MS GC-Q-TOF/MS Detected pesticide 174/25448 343/20418 species/frequency Range of pesticide residue 39.3%-88.0% 28.6%-100% detection rate Total number of 424 Total number of 93 species pesticide species/frequencies species/45,866 pesticide species detected by both techniques times detected by both techniques

(25) TABLE-US-00004 TABLE 4 Basic information of pesticide residues in fruits and vegetables from Beijing, Tianjin, and Hebei (2016) Item LC-Q-TOF/MS GC-Q-TOF/MS Detected pesticide 161/9724 197/9834 species/frequency Range of pesticide residue 20.0%-100.0% 50.0%-100.0% detection rate Total number of 279 Total number of 56 species pesticide species/ species/19,558 pesticide species frequencies detected times detected by both by both techniques techniques

(26) It is shown in Table 3 that in the 22,368 samples from 31 provincial capitals/municipalities in 2012-2015, totally 517 pesticides were detected (wherein, 93 pesticides were detected by both techniques), and the detected frequency was 45,866 times. It is listed in Table 4 that in the 10,190 samples from Beijing, Tianjin, and Hebei in 2016, totally 227 pesticides were detected, and the detected frequency was 19,558 times. It is found in the big data analysis for the general investigation from 31 provincial capitals/municipalities in 2012-2015 and the general investigation from Beijing, Tianjin, and Hebei in 2016 that the safety level of commercial fruits and vegetables in China was essentially assured, at 97% or above pass rate according to the China MRL standards. However, the pesticide residue problem was still severe. It is found in the big data statistical analysis: {circle around (1)} highly toxic or vitally toxic pesticides (e.g., Carbofuran, Isocarbophos, and Methidathion) and prohibited pesticides (e.g., Thimet, Ethoprophos) were still detected frequently, and the detection frequency is 5.5% of the total detection frequency; {circle around (2)} there are about 2.9% samples in which the pesticide residues were exceeding MRLs; {circle around (3)} there are about 0.7%; individual samples in which more than 10 pesticide residues were found {circle around (4)} the quantity of pesticide residue species detected in single specie of fruits and vegetables was 30 or more, and was even about 100 pesticides at the most; {circle around (5)} The detection rates of pesticide residues in common fruits (grape, apple, pear and peach) and vegetables (celery, tomato, cucumber and sweet pepper) were high, and the phenomena of exceeding MRLs were severe, shown in Tables 5 and 6; {circle around (6)} comparing with the MRL standards in advanced countries, the pesticide residue MRLs in China are confronted with a challenge of lower quantity and lower threshold. For example, in the 9,834 detected times of pesticide residues in the general investigation (GC-Q-TOF/MS) from Beijing, Tianjin, and Hebei in 2016, there are only 2,233 corresponding MRL items in the China MRL standards, which is 22.7%. China MRL standards are the lowest among all of the 6 MRL standards, which are much lower than the MRL standards in EU and Japan.

(27) TABLE-US-00005 TABLE 5 Detection results of pesticide residues in 4 types of fruits (grape, apple, pear and peach) and 4 types of vegetables (celery, tomato, cucumber, and sweet pepper) LC-Q-TOF/MS GC-Q-TOF/MS Number of Number of Number of pesticide Number of pesticide Total samples in species in Total samples in species in number which Pesticide which number which Pesticide which of pesticides detection pesticides of pesticides detection pesticides Sample samples are detected rate, % are detected Sample samples are detected rate, % are detected Grape 411 367 89.3 75 Grape 389 316 85.6 1 Apple 628 579 92.2 61 Peach 279 234 83.9  83 Pear 574 397 69.2 52 Pear 437 349 79.9 91 Celery 537 479 89.2 87 Celery 353 341 96.6 132 Tomato 621 547 88.1 81 Cucumber 343 381 87.8 112 Cucumber 591 548 92.7 76 Sweet pepper 369 292 79.1 104

(28) TABLE-US-00006 TABLE 6 MRL analysis of three categories of pesticide residues in 4 types of fruits (grape, apple, pear and peach) and 4 types of vegetables (celery, tomato, cucumber, and sweet pepper) LC-Q-TOF/MS GC-Q-TOF/MS Number of Number of Number of Number of Number of Number of out-of out-of out-of out-of out-of out-of specification specification specification specification specification specification pesticides pesticides pesticides pesticides pesticides pesticides according to according to according to according to according to according to China MRL EU MRL Japan MRL China MRL EU MRL Japan MRL Sample standards standards standards Sample standards standards standards Grape 9 24 25 Grape 3 24 33 Apple 3 17 11 Peach 3 23 30 Pear 4 11 9 Pear 2 24 33 Celery 7 45 36 Celery 8 69 88 Tomato 5 21 21 Cucumber 5 32 37 Cucumber 8 22 22 Sweet pepper 2 19 37

(29) The above detailed description is provided only to describe some feasible embodiments of the present invention rather than to limit the protection scope of the present invention. Any equivalent embodiment or modification implemented without departing from the spirit of the present invention shall be deemed as falling in the protection scope of the present invention.