ASEPTIC CONNECTOR

Abstract

The invention relates to a An aseptic connector comprising a first connection unit (102; 202; 302; 402; 502) provided with a first terminal end surface (104; 204; 304; 404; 504) and a second connection unit (106; 206; 306; 406; 506) provided with a second terminal end surface (108; 208; 308; 408; 508); said first and second terminal end surfaces are each provided with at least one first and second opening (110, 112; 210, 212; 310, 312; 410, 412; 510, 512), which openings are orientated to substantially coincide when said first and second connection units are connected to each other; said first and second openings are sealed by at least one film (114; 214; 314; 414; 514) arranged on said first and second connection units, so that the contact between the film and each connection unit is aseptic; said at least one film is adapted to be mated with a corresponding film on the other connection unit when said first and second connection units are connected to each other; and said mated films are adapted to be pulled out together two and two after mating such that corresponding first and second openings in said first and second terminal end surfaces are mated aseptically. At least two first ports (116; 216; 316; 416; 516) are arranged on the first connection unit, which first ports have fluid connection with the at least one first opening (110; 210; 310; 410; 510) in the first terminal end surface; and in that at least one second port (118; 218; 318; 418; 518) is arranged on the second connection unit, which the at least one second port has a fluid connection with the at least one second opening (112; 212; 312; 412; 512) in the second terminal end surface.

Claims

1. An aseptic manifold comprising, a first connection unit having a first side and a second side that is opposite to the first side, wherein the first side of the first connection unit comprises at least two first ports and the second side of the first connection unit comprises a first terminal end surface, wherein the first terminal end surface is provided with at least two first openings sealed by a first film and each first opening is in fluid communication with a corresponding first port, and a second connection unit having a first side and a second side that is opposite to the first side, wherein the first side of the second connection unit comprises one second port and the second side of the second connection unit comprises a second terminal end surface, wherein the second terminal end surface is provided with at least two second openings sealed by a second film and the second openings are in fluid communication with the one second port, wherein each of the first opening is positioned to coincide with a second opening when the units are connected, and wherein the first film of the first connection unit is adapted to be mated with the second film of the second connection unit such that when the first and the second connection units are connected to each other the mated films are adapted to be pulled out together two and two to allow the first and second terminal end surfaces to mate aseptically.

2. The aseptic manifold of claim 1, wherein the aseptic manifold is a disposable unit.

3. The aseptic manifold of claim 1, wherein a gasket is arranged around each opening and a gasket around a first opening is adapted to mate with a corresponding gasket around a second opening when first and the second connection units mate with each other such that each first opening is sealingly connected with its corresponding second opening.

4. The aseptic manifold of claim 3, wherein a first foam layer is arranged around each gasket of the first terminal surface and a second foam layer is arranged around each gasket of the second terminal surface such that the first foam layer is adapted to be compressed with the second foam layer when two connection units are mated.

5. The aseptic manifold of claim 1, wherein the first film is double folded over the first openings and a single sheet of the uppermost layer of the first film is reaching outside the first terminal end surface as a first tab and the second film is double folded over the second openings and a single sheet of the uppermost layer of the second film is reaching outside the second terminal end surface as the second tab and the first tab and the second tab are adapted to be pulled together to release the films from the connection units.

6. The aseptic manifold of claim 1, wherein at least one of the first and second ports is provided with a valve, which is arranged to control fluid flow through the aseptic manifold.

7. The aseptic manifold of claim 1, further comprising measuring means that comprises a sensor with an optional RFID tag arranged at the aseptic manifold.

8. The aseptic manifold of claim 1, wherein the connection units are provided with orientation specific locking means arranged to mechanically lock the connection units to each other.

9. The aseptic manifold of claim 1, wherein the first terminal surface is provided with two first openings and the second terminal surface is provided with two second openings that coincides with the two first openings when the units are connected.

10. The aseptic manifold of claim 9, wherein the two second openings are in fluid communication with the one second port via “Y” branching inside the second connection unit.

11. The aseptic manifold of claim 1, wherein the first terminal surface is provided with three first openings and the second terminal surface is provided with three second openings that coincides with the three first openings when the units are connected.

12. The aseptic manifold of claim 1, wherein the first terminal surface is provided with four first openings and the second terminal surface is provided with four second openings that coincides with the four first openings when the units are connected.

13. A sterile circuit comprising at least two lengths of tubing each fluidically connected to one of the at least two ports of the first connection unit.

14. The sterile circuit of claim 13, further comprising a container fluidically connected to the at least two lengths of tubing.

15. An aseptic manifold comprising, a first connection unit having a first side and a second side that is opposite to the first side, wherein the first side of the first connection unit comprises at least two first ports and the second side of the first connection unit comprises a first terminal end surface, wherein the first terminal end surface is provided with one opening sealed by a first film and the first opening is in fluid communication with the at least two first ports, and a second connection unit having a first side and a second side that is opposite to the first side, wherein the first side of the second connection unit comprises one second port and the second side of the second connection unit comprises a second terminal end surface, wherein the second terminal end surface is provided with one second opening sealed by a second film and the second opening is in fluid communication with the one second port, wherein the first opening is positioned to coincide with the second opening when the two units are connected, and wherein the first film of the first connection unit is adapted to be mated with the second film of the second connection unit such that when the first and the second connection units are connected to each other the mated films are adapted to be pulled out together two and two to allow the first and second terminal end surfaces to mate aseptically.

16. The aseptic manifold of claim 15, wherein the aseptic manifold is a disposable unit.

17. The aseptic manifold of claim 15, wherein the first film is double folded over the first opening and a single sheet of the uppermost layer of the first film is reaching outside the first terminal end surface as a first tab and the second film is double folded over the second opening and a single sheet of the uppermost layer of the second film is reaching outside the second terminal end surface as the second tab and the first tab and the second tab are adapted to be pulled together to release the films from the connection units.

18. The aseptic manifold of claim 15, wherein at least one of the first and second ports is provided with a valve, which is arranged to control fluid flow through the aseptic manifold.

19. The aseptic manifold of claim 15, further comprising measuring means that comprises a sensor with an optional RFID tag arranged at the aseptic manifold.

20. The aseptic manifold of claim 15, wherein the connection units are provided with orientation specific locking means arranged to mechanically lock the connection units to each other.

21. A sterile circuit comprising at least two lengths of tubing each fluidically connected to one of the at least two ports of the first connection unit.

22. The sterile circuit of claim 21, further comprising a container fluidically connected to the at least two lengths of tubing.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Further aspects, advantages and features of the invention can be derived from the following detailed description of exemplary embodiments of the invention, with reference to the drawings.

[0029] FIG. 1 shows a side view of a first embodiment of an aseptic connector according to the invention,

[0030] FIG. 2 shows a side view of the first embodiment of the aseptic connector in FIG. 1,

[0031] FIG. 3 shows a first connection unit according to the first embodiment in perspective,

[0032] FIG. 4 shows a side view of a second embodiment of an aseptic connector according to the invention,

[0033] FIG. 5 shows a view in perspective of a third embodiment of an aseptic connector according to the invention,

[0034] FIG. 6 shows a view in perspective of a fourth embodiment of an aseptic connector according to the invention,

[0035] FIG. 7 shows a view in perspective of the first embodiment of the aseptic connector according to the invention provided with a clamp means, and

[0036] FIG. 8 shows a view in perspective of a fifth embodiment of an aseptic connector according to the invention.

DETAILED DESCRIPTION

[0037] FIG. 1 shows a first embodiment of an aseptic connector 100 comprising a first connection unit 102 provided with a first terminal end surface 104 and a second connection unit 106 provided with a second terminal end surface 108. Said first and second terminal end surfaces 104, 108 are each provided with at least one first and second opening 110, 112. In the first embodiment two first openings 110 are arranged in the first terminal end surface 104 and two second openings 112 are arranged in the second terminal end surface 108.

[0038] The first and second openings 110, 112 are sealed by at least one film 114 arranged on said first and second connection units 102, 106. The contact surfaces between the film 114 and each connection unit 102, 104 are aseptic. The film 114 is adapted to be mated with a corresponding film 114 on the other connection unit when said first and second connection units 102, 104 are connected to each other. Thereafter, the mated films 114 are adapted to be pulled out together two and two after mating such that corresponding first and second openings 110, 112 in said first and second terminal end surfaces 104, 108 are mated aseptically. The films 114 can e.g. be folded over and be connected to, or form, tabs protruding outside the connection units, which makes the tabs suitable for pulling.

[0039] In the first embodiment disclosed in FIG. 1, two first ports 116 are arranged on the first connection unit 102, which first ports 116 have fluid connection with respective first opening 110 in the first terminal end surface 104. The fluid connection between the first ports 116 and the first openings 110 forms a respective first passage 117 for fluids. On the second connection unit 106 two second ports 118 are arranged, which second ports 118 have a fluid connection with respective second opening 112 in the second terminal end surface 108. The fluid connection between the second ports 118 and the second openings 112 forms a respective second passage 119 for fluids. Therefore, according to the first embodiment the number of openings 110, 112 in the respective terminal end surface 104, 108 corresponds to the number of ports 118, 116 arranged on the connection unit 102, 106.

[0040] Pipes or tubes 120, such as flexible thermoplastic tubes are intended to be connected to the ports 116, 118 by pushing them over the projecting ports 116, 118, so that a mechanical retention of the pipes or tubes 120 is achieved in order to form a leak-tight seal. The pipes or tubes 120 may also be connected to process containers and equipment, such as plastic bags and pumps (not disclosed). The ports 116, 118 may have equal or different diameters, so that the aseptic connector 100 is applicable to a wide array of pipe and tube 120 sizes. In the embodiment disclosed the ports 116, 118 projects out of the connection units 102, 106. However, it is also possible to arrange the ports 116, 118 as apertures (not disclosed) in the connection unit 102, 106, so that the pipes or tubes 120 are pushed into and installed in the aperture.

[0041] A gasket 122 is arranged around each opening 110, 112. Said gasket 122 being adapted to mate with a corresponding gasket 122 or terminal end surface 104, 108 on the other connection unit 102, 106 which the connection unit 102, 106 possibly should be connected with when the films 114 have been released from the connection units 102, 106. The gasket 122 can possibly also be arranged around a suitable number of openings 110, 112. Also, a foam layer 124, such as a compressible foam layer, is arranged around each gasket 122, which foam layer 124 is adapted to be compressed around each gasket 122 when two connection units 102, 106 are mated. The foam layer 124 provided around the gaskets 122 leads to that the units can be pressed together to a first aseptic connection position where the protective films 114 can be removed without exposing the aseptic process side to the environment, which may be non-sterile. The purpose of the compressible foam layers 124 is to provide the required degree of volumetric variability to allow for an expansion of the two opposite foam layers 124 against each other to remain asepsis when removing the adjacent folded films 114 by pulling.

[0042] In FIG. 1 the films 114 are double folded over said openings 110, 112 and a single sheet of the uppermost layer of the film 114 is reaching outside the terminal end surfaces 104, 108 and adapted to be pulled together with another single sheet of another film 114 when the films 114 are released from the connection units 102, 106. The film 114 is suitably provided to the units 102, 106 before the units are subjected to sterilisation. This means that the connection unit 102, 106 with the attached film 114 can be treated in a non sterile environment while the contents of the connection unit 102, 106 confined by its openings and ports including the openings and ports still are kept sterile or aseptic. In FIG. 1 only one film 114 is arranged in each terminal end surface 104, 108. It is however possible to arrange films 114 for each opening in the terminal end surface 104, 108. The connection units 102, 106 are provided with orientation specific locking means 126 arranged to mechanically lock the connection units 102, 106 to each other.

[0043] FIG. 2 shows the first embodiment of the aseptic connector 100 when the first and 25 second connection units 102, 106 are connected. In this position the locking means 126 mechanically locks the connection units 102, 106 to each other. The openings are orientated to substantially coincide when said first and second connection units 102, 106 are connected to each other, which is disclosed in FIG. 2.

[0044] FIG. 3 shows a first connection unit 102 according to the first embodiment in perspective. In the first terminal end surface 104, two first openings 110 are provided. The first openings 110 are arranged to be sealed by the film 114 arranged on the first connection unit 102, so that the contact between the film 114 and the first connection unit 102 is aseptic. Two first ports 116 are arranged on the first connection unit 102, which first ports 116 have fluid connection with the respective first opening 110 in the first terminal end surface 104. A gasket 122 is arranged around each first opening 110 and a foam layer 124 is arranged around each gasket 122, which foam layer 124 is adapted to be compressed around each gasket 122 when two connection units are mated.

[0045] FIG. 4 shows an aseptic connector 200 according to a second embodiment of the present invention. In this second embodiment two first ports 216 are arranged on the first connection unit 202, which first ports 216 have fluid connection with two first openings 210 in the first terminal end surface 204. On the second connection unit 206 only one second port 218 is arranged, which has a fluid connection with two second openings 212 in the second terminal end surface 208. The first and second openings 210, 212 are orientated to substantially coincide when said first and second connection units 202, 206 are connected to each other. In FIG. 4 the films 214 are removed, so that the gaskets 222 arranged around each opening 210, 212 mates with a corresponding gasket 222 on the other connection unit which the connection unit possibly should be connected with when the films 214 have been released from the connection units 202, 206. Also, the foam layers 224 arranged around each gasket 222 are compressed when the two connection units 202, 206 are mated. The aseptic connector 200 according to this second embodiment is arranged as a manifold.

[0046] FIG. 5 shows an aseptic connector 300 according to a third embodiment of the present invention in perspective. In this third embodiment two first ports 316 are arranged on the first connection unit 302, which first ports 316 have fluid connection with only one first opening 310 in the first terminal end surface 304. On the second connection unit 306 only one second port 318 is arranged, which has a fluid connection with only one second opening 312 in the second terminal end surface 308. The first and second openings 310, 312 are orientated to substantially coincide when said first and second connection units 302, 306 are connected to each other.

[0047] FIG. 6 shows an aseptic connector 400 according to a fourth embodiment of the present invention in perspective. In this fourth embodiment three first ports 416 are arranged on the first connection unit 402, which first ports 416 have fluid connection with three first openings 410 in the first terminal end surface 404. On the second connection unit 406 only one second port 418 is arranged, which has a fluid connection with three second openings 412 in the second terminal end surface 408. The first and second openings 410, 412 are orientated to substantially coincide when said first and second connection units 402, 406 are connected to each other. In this embodiment also at least one of the first ports 416 is provided with a valve 428, which is arranged to control fluid flow through the first connection unit 402 and the aseptic connector 400. The valve 428 can be of the type which opens and closes the port 416. However, it is also possible to arrange the valve as a three-way valve (not disclosed).

[0048] In FIG. 6 the aseptic connector 400 may be provided with measuring means 436 comprising sensors for measuring and monitoring for example pressure, velocity in flow, temperature and conductivity. Also, radio frequency identification (RFID) tags may be used in combination with the sensors in the measuring means 436. The measuring means 436 may be located within the connector 400 and in contact with the fluid in the connector 400, or located on a location outside the connector 400. The RFID tags may communicate with a computer (not disclosed) for collecting information about the measured values. Preferred geometries of the measurement means 436 are round or circular, but other geometries can be used for similar purposes.

[0049] In FIG. 7 the aseptic connector 100 according to the first embodiment is provided with a clamp 130, which at least partially encloses the connector 100. The clamp 130 comprises two shells 132 which are arranged at the periphery of first and second connection units 102, 106 and locked with a strap 134. The clamp 130 urges the first and second connection units 102, 106 in a direction to each other and prevents any leakage of fluid from the aseptic connector 100. The clamp 130 also prevents the first and second connection units 102, 106 to be unintentional disconnected.

[0050] FIG. 8 shows an aseptic connector 500 according a fifth embodiment. In addition to the first embodiment above the aseptic connector 500 according the fifth embodiment has four first ports 516 arranged on the first connection unit 502, which first ports 516 have fluid connection with four first openings 510 in the first terminal end surface 504. On the second connection unit 506 also four second ports 518 are arranged, which have a fluid connection with four second openings 512 in the second terminal end surface 508. The first and second openings 510, 512 are orientated to substantially coincide when said first and second connection units 502, 506 are connected to each other. The first and second openings 510, 512 are sealed by films 514 arranged on said first and second connection units 502, 506. A gasket 522 is arranged around each first opening and a foam layer 524 is arranged around each gasket 522. In the fifth embodiment the films 514, gaskets 522 and foam layer 524 are disclosed when arranged at the second connection unit 506. However, films 514, gaskets 522 and foam layer 524 are also arranged at the first connection unit 502, but not disclosed in FIG. 8. The first and second connection units 502, 506 according to the fifth embodiment have a substantial rectangular configuration in comparison to the substantial circular configuration of the connection units in the embodiments above.

[0051] Preferably, the aseptic connector 100; 200; 300; 400; 500 according to the invention is a disposable unit, i.e. adapted to be used only once. One advantage with disposable systems is that there is no need for cleaning and bioburden control before using the systems because disposable systems are already aseptic in some degree and they should not be used again and need therefore not be cleaned between uses. Therefore the aseptic connector 100; 200; 300; 400; 500 according to this invention is particularly interesting in disposable systems.

[0052] In a further aspect of the invention, a sterile circuit is disclosed, which comprises at least one first connector unit 102; 202; 302; 402; 502 as discussed above and at least two lengths of tubing 120; 220 fluidically connected to the unit, preferably to the ports 116; 118; 216; 316; 416; 516; 518. The sterile circuit may be presterilized, e.g. by irradiation or autoclaving and it may be packaged in a sterile container, such as e.g. a sterile pouch or bag. The sterile circuit may further comprise at least one container, such as a storage bag or a bioreactor bag, which is fluidically connected to at least one of the tubing lengths 120; 220. The first connector unit of the sterile circuit may be aseptically connected to a second connector unit, which may, or may not, form a part of a second sterile circuit with at least one length of tubing fluidically connected to the second connector unit.

[0053] The provision of sterile circuits greatly facilitates the setup of complex sterile systems, such as bioreactors with multiple lines for feeding nutrients, gases etc and for removal of culture fluid and/or metabolites etc. One sterile circuit may be attached to one bioreactor via one or more lengths of tubing or it may be attached to a plurality of bioreactors. The latter is particularly suitable for screening experiments such as high throughput screening experiments where a large number of small bioreactors are used and it is of imperative need to have a compact system for supply and removal of fluids.

[0054] The word aseptic used in this description and in the claims shall have a broad definition, i.e. include any level of bioburden control. The bioburden control or asepsis can be measured as organisms/ml or CFU (colony forming units). In one embodiment of the invention the level of asepsis should be below 100 CFU/ml. The latter corresponds to bioburden control levels required for food grade products. Low levels of bioburden can be achieved by sterilisation processes. For example the aseptic connector 100; 200; 300; 400; 500 of the invention can be subjected to gamma sterilization. Other possible methods are autoclaving or bioburden control by ethylene dioxide.

[0055] In all embodiments described above parts and surfaces being in contact with a process fluid are suitably selected from materials that are in accordance with typical material requirements in (bio-)pharmaceutical manufacturing or food grade quality. For example, materials are suitably in compliance with USP Class VI and 21 CFR 177. Furthermore they are suitably of animal-free origin and compliance to EMEA/41O/01.

[0056] Features and components of the different embodiments above may be combined within the scope of the invention.