COMPOSITIONS AND METHODS FOR REPELLING COLEOPTERANS

Abstract

Compositions and methods for repelling coleopterans. The compositions contain a substrate and an anti-aggregation pheremone intermixed within the substrate. The pheremone may be verbenone, methyl salycylate, wintergreen oil, or combinations thereof.

Claims

1. A system for repelling coleopterans, the system comprising: a polymeric or wax substrate selected from the group consisting of a wax emulsion, microspheres, a latex solution, hot melt glue, a resin, and plastic flakes; and an anti-aggregation pheremone intermixed within the substrate, wherein the anti-aggregation pheremone is selected from the group consisting of verbenone, methyl salicylate, wintergreen oil extracted from the leaves of a plant within the Gaultheria genus, an extract and infusion from the botanical orders Alismatales, Apiales, Arecales, Asparagales, Asterales, Brassicales, Canellales, Caryophyllales, Cycadales, Dipsacales, Ericales, Fabales, Gentianales, Lamiales, Laurales, Magnoliales, Malpighiales, Malvales, Myrtales, Pinales, Poales, Ranunculales, Rosales, Sapindales, Solanales, Theales or Zingiberales, and combinations thereof.

2. The system of claim 1, further comprising an insect toxicant.

3. The system of claim 1, wherein the substrate is a wax emulsion formed by: melting a wax; and adding to the melted wax, an oil, an emulsifier, a preservative, and water heated above the melting temperature of the wax to form the wax emulsion.

4. The system of claim 1, wherein the anti-aggregation pheremone is a combination of verbenone and methyl salicylate.

5. The system of claim 1, wherein the anti-aggregation pheremone is a combination of verbenone and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

6. The system of claim 1, wherein the anti-aggregation pheremone is a combination of verbenone, methyl salycylate, and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

7. The system of claim 1, wherein the coleopteran is from the genera Dendroctonus spp., Rhynchophorus spp., Hypothenemus spp., Xylosandrus spp., Cosmopolites spp., Alphitobius spp., Sphenophorus spp., and Anthonomus spp.

8. The system of claim 7, wherein the coleopteran is the coffee berry borer.

9. The system of claim 7, wherein the coleopteran is Cosmopolites sordidus.

10. The system of claim 7, wherein the coleopteran is Alphitobius diaperinus.

11. The system of claim 7, wherein the coleopteran is Sphenophorus levis.

12. The system of claim 7, wherein the coleopteran is Anthonomus grandis.

13. A method of repelling coleopterans in a region, the method comprising administering a system to the region, the system comprising: a polymeric or wax substrate selected from the group consisting of a wax emulsion, microspheres, a latex solution, hot melt glue, a resin, and plastic flakes; and an anti-aggregation pheremone intermixed within the substrate, wherein the anti-aggregation pheremone is selected from the group consisting of verbenone, methyl salicylate, wintergreen oil extracted from the leaves of a plant within the Gaultheria genus, an extract and infusion from the botanical orders Alismatales, Apiales, Arecales, Asparagales, Asterales, Brassicales, Canellales, Caryophyllales, Cycadales, Dipsacales, Ericales, Fabales, Gentianales, Lamiales, Laurales, Magnoliales, Malpighiales, Malvales, Myrtales, Pinales, Poales, Ranunculales, Rosales, Sapindales, Solanales, Theales or Zingiberales, and combinations thereof.

14. The method of claim 13, wherein the anti-aggregation pheremone is a combination of verbenone and methyl salicylate.

15. The method of claim 13, wherein the anti-aggregation pheremone is a combination of verbenone and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

16. The method of claim 13, wherein the anti-aggregation pheremone is a combination of verbenone, methyl salycylate, and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

17. The method of claim 13, wherein the coleopteran is from the genera Dendroctonus spp., Rhynchophorus spp., Hypothenemus spp., Xylosandrus spp., Cosmopolites spp., Alphitobius spp., Sphenophorus spp., and Anthonomus spp.

18. A method for dislodging coffee berry borer from a coffee berry, the method comprising administering a system on or in the vicinity of the coffee berry, the system comprising: a polymeric or wax substrate selected from the group consisting of a wax emulsion, microspheres, a latex solution, hot melt glue, a resin, and plastic flakes; and an anti-aggregation pheremone intermixed within the substrate, wherein the anti-aggregation pheremone is selected from the group consisting of verbenone, methyl salicylate, wintergreen oil extracted from the leaves of a plant within the Gaultheria genus, an extract and infusion from the botanical orders Alismatales, Apiales, Arecales, Asparagales, Asterales, Brassicales, Canellales, Caryophyllales, Cycadales, Dipsacales, Ericales, Fabales, Gentianales, Lamiales, Laurales, Magnoliales, Malpighiales, Malvales, Myrtales, Pinales, Poales, Ranunculales, Rosales, Sapindales, Solanales, Theales or Zingiberales, and combinations thereof.

19. The method of claim 18, wherein the anti-aggregation pheremone is a combination of verbenone and methyl salicylate.

20. The method of claim 18, wherein the anti-aggregation pheremone is a combination of verbenone and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

21. The method of claim 18, wherein the anti-aggregation pheremone is a combination of verbenone, methyl salycylate, and wintergreen oil extracted from the leaves of a plant within the Gaultheria genus.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

[0044] These and other features and advantages of the various embodiments disclosed herein will be better understood with respect to the following description and drawings, in which like numbers refer to like parts throughout, and in which:

[0045] FIG. 1 is a graph showing coffee berry infestation between control groups and those being treated with a composition as described herein; and

[0046] FIG. 2 is a graph showing coffee berry infestation as described in FIG. 1 over a period of time.

DETAILED DESCRIPTION

[0047] The detailed description set forth below is intended as a description of the presently preferred embodiment of the invention, and is not intended to represent the only form in which the present invention may be constructed or utilized. The description sets forth the functions and sequences of steps for constructing and operating the invention. It is to be understood, however, that the same or equivalent functions and sequences may be accomplished by different embodiments and that they are also intended to be encompassed within the scope of the invention.

[0048] Semiochemical formulations should exhibit a zero-order-release rate and sustain release levels above a certain threshold for a long period of time, wherein release levels below threshold would only have a negligible disruptive effect on the behavior of the target insect. With a couple of exceptions, when formulated with less than 10% active ingredient (AI) Specialized Pheromone and Lure Application Technology (SPLAT) consistently provides a near zero-order-release rate of the semiochemical, with negligible flash-off around the time of application.

[0049] The initial research and development that culminated into the existing SPLAT technology was done using Grapholita molesta, the Oriental fruit moth (OFM), a serious pest of apples worldwide, as the model insect. When formulated with less than 10% OFM pheromone, SPLAT consistently provided a near zero-order-release rate with negligible flash-off. Field trials in large commercial apple operations in South America indicated that SPLAT formulations containing 15 g pheromone per acre sustained nearly complete trap shutdown for over 180 days, which actually translated in a significant reduction of OFM fruit damage, as compared to that found in the grower's traditional chemical control. Analysis of the field “aged” SPLAT indicated that there were different levels of pheromone remaining in the point source of SPLAT by the end of the 180 day trial period, and it was related to the position of the dollop in the canopy (receiving more or less solar incidence) as well as the actual size of the dollop analyzed: pheromone in microdollops was undetectable, whereas 1 g dollops still contained 5-10% and 5 to 10 g dollops retained between 10 and 25% AI.

[0050] In the case of CBB, the present disclosure envisions a SPLAT and pheromone based repellent formulation that provides acceptable long-term repellency while minimizing any potential deleterious impacts. The application of SPLAT and verbenone can occur at the onset of coffee berry formation and by remaining in place emitting pheromone would repel existing and emerging adults from further damaging developing coffee berries.

[0051] This novel formulation, a SPLAT and verbenone formulation that is repellent, can be applied using conventional defensive application apparatus, will last for 6 months or longer in the field, releases nearly all the pheromone it contains, and is biodegradable and safe.

[0052] This formulation provides coffee farmers with a functional system and substantial savings. The cost of SPLAT with 10 g verbenone/acre will be about $30 dollars per acre, which represents a savings of $30-60 per acre just considering the cost of BOTANIGARD®ES. Now, if this formulation also substitutes the customary two treatments of BOTANIGARD®ES at a cost of $60-90/acre, then the savings will be $60-120/acre.

[0053] Our calculation of the savings is very conservative. It does not take in account the additional savings realized because of the simplification of the current cultural control CBB operation, a formulation with such a long life that it allows for a single application for the entire CBB cycle. More savings will be achieved by elimination of crisis situations and their associated cost. Furthermore, we believe we overestimate the cost of manufacturing SPLAT. It is probably high because it is based on the price we currently pay our suppliers, which do not reflect the discounts available from commercial suppliers when we purchase the raw materials in large bulk quantities. Furthermore the efficiency of the production line increases with the larger volumes, needing less worker hours per volume of SPLAT produced. These additional savings have not been accounted for.

[0054] Because of its repellent action, SPLAT and verbenone may provide population protection not only in areas of existing populations but also areas with low and no population densities, such as newly established plantations, providing a huge benefit to the coffee industry and population in general.

[0055] It is believed that the ideal verbenone formulation should be applied using conventional spray equipment, have a duration of at least two to six months, stick to the foliage where it lands, quickly acquire rain-fast qualities, protect the pheromone from degradation, work synergistically with adulticides so that it can possibly control CBB populations at low as well as at high densities, be biodegradable, if possible organic, not damage private property, and last but not least the formulation should be inexpensive for its adoption to be not only technically, but also economically feasible.

[0056] The present invention is expected to meet all of the desired factors by providing an optimal semiochemical solution for the effective management of CBB independent of population density. The overall objective of this invention is to provide effective season-long field control of CBB populations using a flowable wax emulsion system (SPLAT) that delivers the anti-aggregation pheromone verbenone. We formulated SPLAT and verbenone using flowable wax emulsions of different characteristics in order to determine in the lab the emission rate and stability of the pheromone. Two of the optimized formulations were submitted to laboratory and field trials to which we added high CBB pressure. Field aged samples of the SPLAT formulations were analytically quantified to determine residual stability and effectiveness of pheromone components.

[0057] Some of the goals of the invention include: 1) a formulation having a duration of six months while being protected from degradation while dispensing verbenone; 2) a formulation that works synergistically with killing agents; 3) a formulation of SPLAT that works with conventional aerial spray equipment, sticks to foliage and quickly becomes rain-fast; and 4) a formulation that controls CBB populations at low as well as high densities. However, it is contemplated that in some embodiments of the invention only some, or even none of the goals may be achieved.

[0058] There are no verbenone formulations in the market today that have high longevity and that can be applied using conventional spray equipment. The present invention is an optimal semiochemical solution for the effective management of CBB independent of population density. The present invention is effective in season-long field control of CBB populations using a flowable wax emulsion system (SPLAT) that delivers the anti-aggregation pheromone verbenone. The release rate of verbenone from SPLAT, 10% of pheromone in the SPLAT composition, applied in the field as point sources at three sizes, 1 g, 7 g and 17 g each was measured. The SPLAT formulations retained and continued emitting pheromone for 120 days, with the formulations still retaining 30%-80% of its pheromone (depending on dose and point source size). This suggests that these formulations would probably last another 30 to 60 days in the field (depending on dose and point source size). This indicates that if the formulation is sprayed in the field at the time of coffee berry development, it will last through harvest. This allows for the first time a formulation that can be sprayed to control CBB through the emission of effective rates of pheromone during adult flight.

[0059] By targeting CBB with an organic, safe, and effective formulation, non-target organisms will be minimally affected by any extra insecticide use, so insect species diversity will be retained where the semiochemical formulation is used. This in turn will conserve vertebrate animals that depend upon insects for their diet.

[0060] In one embodiment of SPLAT and verbenone the pheromone lasts for the whole season, but just for the coffee berry production season, not longer, to avoid unwanted residual problems and exposure to non-target species. The systems and methods of the present invention may be utilized for a plethora of other pests and invasive species (e.g., Mountain Pine Beetle, Southern Pine Beetle, Western Pine Beetle and others).

[0061] Our testing involved application of approximately 0.2 g of SPLAT Verb in approximately a 6-inch petri dish. (SPLAT Verb is loaded with 10% verbenone by weight.) This area would correspond to 0.0000045ac of land; therefore, our field application rate would be approximately 44 kg of SPLAT Verb (4,400 g of verbenone) per acre. Coffee berries, commonly used to rear CBB, were placed in the petri dish. The 0.2 g of SPLAT Verb was introduced into the sealed petri dish. We then went on to measure the times when CBB dislodged or exited the berry. The number dislodged is a summation of the total dislodged at that point in time. Our data (as shown in Table 3) verifies that CBB exited their preferred host and, after dislodging, maintained high activity levels without re-entering the berries. This data serves as the first confirmation of verbenone's repellant activity.

TABLE-US-00003 TABLE 3 Number Activity Level Time Dislodged Outside of Berry  4 min 51 sec 2 High  7 min 17 sec 3 High 15 min 32 sec 4 High 19 min 25 sec 6 High 22 min 21 sec 7 High 28 min 38 sec 8 High 32 min 32 sec 9 High Total in 37 min 9 High

[0062] Alcohol is a strong CBB attractant. Further testing of SPLAT Verb shows (as can be seen in FIGS. 1 and 2) that the addition of verbenone reduces the level of attraction of CBB even to traps with alcohol to levels below the control (trap without attractant or any other semiochemical).

[0063] The SPLAT and verbenone pheromone release formulation, a novel, amorphous, flowable emulsion can be applied as microliter point sources all the way to dollops of tens of grams. The SPLAT wax dispenser formulations of this invention belong to a “matrix-type” or “monolithic” category of controlled-release devices. These “matrix-type” or “monolithic” dispensers are defined as devices where the active ingredient is dispersed or dissolved in a polymer matrix. Release of the active ingredient from a monolithic device occurs by diffusion and can be described macroscopically by Fick's Law. Fick's law states that the movement of a molecule by diffusion is directly proportional to the concentration of that molecule in a system. Microscopically, if we follow the movement of a molecule of an active agent through a matrix, this molecule can begin its journey in one of two ways. If it is dispersed in the matrix, it begins its journey by dissociating from other molecules in its crystal cell and solubilizing into the polymer phase. If it is dissolved in the matrix, then this step is bypassed. The molecule then diffuses through amorphous regions in the matrix that comprise the free volume of the system. The molecule can move through the matrix in one of two ways as well. If it is very small compared to the size of the amorphous spaces in the matrix, then it will diffuse through the matrix by moving from one such space to another. If it is very large compared to the size of those spaces, then segments of the polymer comprising the matrix will have to be rearranged for diffusion of the active agent molecule to occur. Crystalline regions in the matrix are virtually impermeable to molecules of the active agent. Upon reaching the surface of the matrix, it will be released into the environment.

[0064] A series of factors influences the rate of release of an active agent from a monolithic device and includes properties of the matrix material as well as properties of the active agent. The temperature of the matrix influences release of the active agent; at higher temperatures the free volume is increased, and diffusion occurs faster. At lower temperatures, the free volume is decreased, and diffusion is slower. The thermal history of a polymer can also increase or decrease the free volume of the system and lead to changes in the diffusional rate of an active agent.

[0065] The property of the active agent having the greatest influence on its release rate is its molecular weight. Generally, larger molecules take more time to make their way through the free space of a matrix. Branching in a molecule can also decrease its rate of diffusion through a matrix. The partition coefficient of the active agent between the matrix and the environment can also influence the release rate of that agent. If the agent readily partitions to the environment, then its rate of release will be diffusion-controlled and first order. If, however, partitioning of the active agent to the environment is relatively slow, then its partition coefficient will determine its release rate from the matrix and the device will exhibit zero order release kinetics. The partitioning of the active agent to the environment is a function of the solubility of the active agent in the matrix; compounds more soluble in the matrix partition to the environment more slowly. SPLAT paraffin emulsions in a field environment exhibit diffusion-controlled release. The surface area of the device also influences its release rate. Paraffin dispensers with larger surface areas release active agent at faster rates.

[0066] The release rate of a SPLAT formulation containing a fixed amount of semiochemical can be modulated simply by changing a few of parameters of the formulation, which includes the type of components used (e.g. the wax composition, the emulsifiers used), their proportion in the formulation (e.g., percentage of water, oil or wax content), the stage in the manufacturing the different components are added, the rheology, and finally the characteristics of application of the SPLAT in the field (e.g., applied as microdollops of 1-10 ug each or large dollops of 10 g each).

[0067] The result is a semiochemical formulation that is extremely malleable and that fits many needs and uses that cannot be supported by any other commercial formulation present in the market.

[0068] A 30% paraffin wax emulsion was made consisting of 30% paraffin wax (Gulf Wax, Royal Oak Sales, Inc., Roswell, Ga.), 4% soy oil (Spectrum Naturals, Inc., Petaluma, Calif.), 2% Span 60 (Sorbitan monostearate, Sigma-Aldrich Co., St. Louis, Mo.), 1% vitamin E α-tocopherol, Sigma Chemical Co., St. Louis, Mo.), and 58% distilled water. A 45% microcrystalline wax emulsion consisting of 45% microcrystalline wax (Blended Waxes, Inc., Oshkosh, Wis.), 6% soy oil, 3% Span 60, 1% vitamin E, and 40% distilled water was also made.

[0069] The wax is melted (paraffin: 60-65° C.; microcrystalline: 78-80° C.) and water heated above the melting temperature of the wax (paraffin emulsion: 65-70° C.; microcrystalline emulsion: 78-88° C.). The soy oil, Span 60, and vitamin E are added to the melted wax and thoroughly mixed, followed by the addition of the hot water. This mixture is then poured into a industrial laboratory blender. The emulsion is immediately blended, then placed in a cold water bath, and mixed every 15 minutes until the solution had cooled to 25-30° C. when it is placed in a plastic bucket and stored until use.

[0070] Just prior to use, 0.03% (3 g), 1.0% (10 g) and 3.0% (30 g) by weight of emulsion of racemic verbenone is thoroughly mixed into the emulsion using a high sheer lab mixer.

[0071] Preliminary work with generic SPLAT formulations containing 3% racemic verbenone using flow cells indicates that it releases pheromone at a very constant level for long periods. To show the release of AI semiochemicals within SPLAT, we collected the effluvia from 5 g SPLAT GM 3%, containing 150 mg disparlure (AI) and found that it released disparlure at a rate of 44.06±13.08 ug/day for over ca. 170 days. As a comparison, 5 g of Disrupt II (a prior art formulation), containing 894 mg disparlure emit 51.45±2.33 ug/day. It is important to realize that although both flow chambers had 5 grams of formulation, Disrupt II actually had six times more pheromone than SPLAT while it released only 15% more pheromone than SPLAT, a difference that probably has no biological effect to speak of. These results suggest that SPLAT is a much more efficient formulation than those known in the prior art; actually six times more efficient. As such, one would need to apply six times less AI using SPLAT than if using prior art formulations. Usually the most expensive component of a mating disruption formulation is the active ingredient. Accordingly, SPLAT is believed to be substantially less expensive than the formulations of the prior art.

[0072] With SPLAT, one can change the consistency of the emulsion by changing the proportion of components, or by changing the rheology of the mixing of the components. The word “rheology” normally refers to the flow and deformation of “non-classical” materials such as rubber, molten plastics, polymer solutions, slurries and pastes, electrorheological fluids, blood, muscle, composites, soils, and paints. These materials can exhibit varied and striking external and internal structures due to their rheological properties that classical fluid mechanics and elasticity cannot describe. Our experience is that the SPLAT formula with 45% microcrystalline wax emulsion (45% microcrystalline wax, 6% soy oil, 3% Span 60, 1% vitamin E, and 40% distilled water) can be mechanically applied and that it adheres quickly to the vegetation, and as long as it has a couple hours to settle, it becomes rain fast.

[0073] Specialized Pheromone & Lure Application Technology (SPLAT) is a base matrix formulation of biologically inert materials used to control the release of semiochemicals and/or odors with or without pesticides. Extensive research on SPLAT using a variety of lures demonstrates that this matrix emits semiochemicals at effective pest suppression levels for a time interval ranging from 2-16 weeks. Having a wide range of viscosities and application methods (e.g. applicator sprays, aerial applicator sprays, caulking gun type tubes, etc.), SPLAT increases productivity by mechanizing the application of pheromone dispensing points. The amorphous and flowable quality of this highly adaptable product allows for an easy transition from small-scale manual applications to large-scale mechanical applications.

[0074] Additional experimentation as described below shows that the compositions and methods described herein function with numerous coleopterans. Testing was done to evaluate the effect of SPLAT plus Verbenone (+) and Wintergreen Oil on the Lesser Mealworm, Alphitobius diaperinus. To do so, the composition was placed in traps containing a substrate attractant to see whether there would be a reduction in captured insects. Four repetitions were used for each treatment. To avoid any interference between the treatments, a minimum distance of 25 meters was used to separate each trap. The treatments were prepared in three formats: 01 had the trap with poultry litter, 02 had the trap with poultry litter and the SPLAT plus Verbenone (+) and Wintergreen Oil composition, and 03 was merely the trap. The number of captured insects after two weeks were determined. The results are shown in Table 4 below. As such, it can be seen that the presence of SPLAT with Verbenone (+)+Wintergreen Oil reduce the number of insects captured in traps with an attractant source (poultry litter), showing a repellent effect for the species Alphitobius diaperinus.

TABLE-US-00004 TABLE 4 Treatment R1 R2 R3 R4 Average 01 48 76 29 66 54.7 02 3 0 5 2 2.5 03 0 0 0 0 0

[0075] Testing was done to evaluate the effect of SPLAT plus Verbenone (+) and Wintergreen Oil on the behavior of the Banana root borer, Cosmopolites sordidus. To do so, the composition was placed in traps containing an attractant source (pheromone traps) to see whether there would be a reduction in captured insects. Four repetitions were used for each treatment. To avoid any interference between the treatments, a minimum distance of 25 meters was used to separate each trap. The treatments were prepared in three formats: 01 had the trap with pheromone, 02 had the trap with pheromone and the SPLAT plus Verbenone (+) and Wintergreen Oil composition, and 03 was merely the trap. The number of captured insects after four weeks were determined. The results are shown in Table 5 below. As such, it can be seen that the presence of SPLAT with Verbenone (+)+Wintergreen Oil reduce the number of insects captured in traps with an attractant source (pheromone), showing a repellent effect for the species Cosmopolites sordidus.

TABLE-US-00005 TABLE 5 Treatment R1 R2 R3 R4 Average 01 18 22 38 24 25.5 02 1 0 2 0 0.75 03 0 0 0 1 0.25

[0076] Testing was done to evaluate the effect of SPLAT plus Verbenone (+) and Wintergreen Oil on the behavior of the Sugarcane weevil, Sphenophorus levis. To do so, the composition was placed in traps containing an attractant source (fermented sugarcane) to see whether there would be a reduction in captured insects. Four repetitions were used for each treatment. To avoid any interference between the treatments, a minimum distance of 25 meters was used to separate each trap. The treatments were prepared in three formats: 01 had the trap with fermented sugarcane, 02 had the trap with fermented sugarcane and the SPLAT plus Verbenone (+) and Wintergreen Oil composition, and 03 was merely the trap. The number of captured insects after six weeks were determined. The results are shown in Table 6 below. As such, it can be seen that the presence of SPLAT with Verbenone (+)+Wintergreen Oil reduce the number of insects captured in traps with an attractant source (fermented sugarcane), showing a repellent effect for the species Sphenophorus levis.

TABLE-US-00006 TABLE 6 Treatment R1 R2 R3 R4 Average 01 11 7 26 12 14 02 1 0 0 0 0.25 03 0 0 0 0 0

[0077] Testing was done to evaluate the effect of SPLAT plus Verbenone (+) and Wintergreen Oil on the behavior of the Boll Weevil, Anthonomus gradis. To do so, an “X” olfactometer was used to realize the choice tests. Twenty insects were used per treatment. Namely, two of the four extremities received a control treatment (blank SPLAT) and the other two the specific composition. The treatments were prepared in two formats: 01 the control, made up of Blank SPLAT and a pheromone (grandlure) and 02 the test, made up of Blank SPLAT, pheromone (grandlure) with the Verbenone (+) and Wintergreen Oil composition. The number of insects that moved into each treatment extremity of the olfactometer was recorded. The results are shown in Table 7 below. As such, it can be seen that he presence of SPLAT with Verbenone (+)+Wintergreen Oil reduce choice number of insects when used in combination with an attractant source (pheromone), showing a repellent effect for the species Anthonomus grandis.

TABLE-US-00007 TABLE 7 Control (Blank SPLAT) Pheromone (Grandlure) Treatment 01 02 18 Pheromone (Grandlure) + Control (Blank SPLAT plus Verbenone (+) + Treatment 02 SPLAT) Wintergreen Oil 16 04

[0078] The above description is given by way of example, and not limitation. Given the above disclosure, one skilled in the art could devise variations that are within the scope and spirit of the invention disclosed herein, including various ways of administering the verbenone. Further, the various features of the embodiments disclosed herein can be used alone, or in varying combinations with each other and are not intended to be limited to the specific combination described herein. Thus, the scope of the claims is not to be limited by the illustrated embodiments.