Abstract
A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample (10) is provided, the cartridge (300,400, 500) being adapted to fixedly hold a sample chamber (216, 218, 220) at a sample chamber position (320, 420, 520) in the cartridge (300, 400, 500), a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) being provided in the cartridge (300, 400, 500) and extending from a surface of the cartridge (300, 400, 500) to the sample chamber position, and the cartridge (300, 400, 500) comprising a baffle structure (350, 450, 550, 571) at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position in the cartridge (300, 400, 500). A corresponding method in which the high pressure freezing cartridge (300, 400, 500) is used is also part of the present disclosure.
Claims
1. A high pressure freezing cartridge (300, 400, 500) for use in vitrification of a biological sample (10), the cartridge (300, 400, 500) being adapted to fixedly hold a sample chamber at a sample chamber position (320, 420, 520) inside the cartridge (300, 400, 500), the cartridge (300, 400, 500) comprising: a refrigerant channel arrangement comprising at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) extending from a surface of the cartridge (300, 400, 500) to the sample chamber position; and a baffle structure (350, 450, 550, 571) at a baffle position, the baffle structure (350, 450, 550, 571) being adapted to interact with a refrigerant stream in the at least one refrigerant channel before a refrigerant of the refrigerant stream reaches the sample chamber position (320, 420, 520) in the cartridge (300, 400, 500).
2. The cartridge (300, 400, 500) according to claim 1, wherein the cartridge (300, 400, 500) comprises a plurality of cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) positionable into an operational configuration, the plurality of cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) including two holding elements (302, 304, 402, 404, 502, 504) and a middle plate (306, 406, 506) sandwiched between the two holding elements (302, 304, 402, 404, 502, 504) in the operational configuration of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506).
3. The cartridge (300, 400, 500) according to claim 2, wherein the middle plate (306, 406, 506) is a flat element comprising an opening (316, 416, 516) adapted to form the sample chamber by fitting enclosing elements (218) into the opening (316, 416, 516).
4. The cartridge (300, 400, 500) according to claim 2, wherein at least a majority of an outer surface of the cartridge (300, 400, 500) corresponds to a cylindrical shape, wherein a part of an outer surface of each of the two holding elements (302, 304, 402, 404, 502, 504) corresponds to the cylindrical shape when the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) are in the operational state, and wherein a further part of the outer surface of each of the two holding elements (302, 304, 402, 404, 502, 504) not corresponding to the cylindrical shape is adapted to be brought into contact with at least a part of a respective surface of the middle plate (306, 406, 506) in the operational state of the cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506).
5. The cartridge (300, 400, 500) according to claim 2, wherein the two holding elements (302, 304, 402, 404, 502, 504) are identically shaped.
6. The cartridge (300, 400, 500) according to claim 2, wherein the refrigerant channel arrangement comprises at least one refrigerant channel (208, 308, 408, 462, 464, 466, 468, 508, 562, 564, 566, 569) formed by one or more grooves in at least one of the two holding elements (302, 304, 402, 404, 502, 504).
7. The cartridge (300, 400, 500) according to claim 2, wherein the baffle structure (350, 450, 550) is configured as a flat disc with an engaging element (351, 451, 551), the baffle structure (350, 450, 550) being positioned or fixedly held by at least one of the plurality of cartridge elements (302, 304, 306, 402, 404, 406, 502, 504, 506) at the baffle position by engaging the engaging element (351, 451, 551) to the at least one cartridge element (302, 304, 306, 402, 404, 406, 502, 504, 506).
8. The cartridge (300) according to claim 7, wherein the baffle position corresponds to a position at the surface of the cartridge (300) from which the at least one refrigerant channel (308) of the refrigerant channel arrangement extends to the sample chamber position.
9. The cartridge (300) according to claim 8, wherein the refrigerant channel arrangement comprises at least one refrigerant feeding channel (352) extending from a further position at the surface of the cartridge (300) to communicate with the at least one refrigerant channel (308) at a position downstream of the baffle structure (350).
10. The cartridge (300) according to claim 8, wherein a recess (354) at the surface of the cartridge (300) is provided, wherein the recess (354) is configured to receive the baffle structure (350).
11. The cartridge (400, 500) according to claim 6, wherein the refrigerant channel arrangement comprises a section formed by one or more refrigerant channels (408, 508) extending from the surface of the cartridge (400, 500) to the baffle position and another section including one or more further refrigerant channels (462, 464, 562, 564) extending from the baffle position to the sample chamber position.
12. The cartridge (400, 500) according to claim 11, wherein the holding elements (402, 404, 502, 504) comprise recesses (456, 556) to receive the baffle structure (450, 550), and wherein the middle plate (406, 506) comprises a bore (458, 558) through which the baffle structure (450, 550) extends when the baffle structure (450, 550) is received in the recesses (456, 556) of the holding elements (402, 404, 502, 504).
13. The cartridge (400, 500) according to claim 11, wherein the baffle structure (450, 550) comprises holes (460, 560) arranged at positions which are different from positions of straight line projections of the one or more refrigerant channels (408, 508) extending from the surface of the cartridge (400, 500) to the baffle structure (450, 550).
14. The cartridge (500) according to claim 1, wherein the baffle structure (571) is provided as a protrusion integrally with the material of at least one of the holding elements (502, 504).
15. A method for high pressure freezing to vitrify a biological sample (10), the method comprising: providing a cartridge (300, 400, 500) according to claim 1; positioning the cartridge (300, 400, 500) in a high pressure freezing device (100); and passing a cryogenic refrigerant at an elevated pressure through the refrigerant channel arrangement of the cartridge (300, 400, 500) to withdraw heat from the sample (10).
Description
BRIEF DESCRIPTION OF THE DRAWING VIEWS
[0033] Further features of the present disclosure will be described in connection with the appended drawings in which embodiments of the disclosure are described vis-à-vis the prior art. Be it noted that specific features of the embodiments described in connection with the drawings and described above can be used in any combination and/or isolatedly without leaving the scope of the disclosure.
[0034] FIG. 1 illustrates a high-pressure freezing system usable in the present disclosure.
[0035] FIG. 2 illustrates a sample cartridge held in a holder usable in the present disclosure.
[0036] FIG. 3 shows a sample cartridge not according to the disclosure in an exploded view.
[0037] FIG. 4 shows middle plate of FIG. 3 in a more detailed view.
[0038] FIG. 5 shows a sample cartridge according to an embodiment of the disclosure.
[0039] FIG. 6 shows a sample cartridge according to an embodiment of the disclosure.
[0040] FIG. 7 shows a sample cartridge according to an embodiment of the disclosure.
[0041] In the Figures, like elements are indicated with identical reference numerals. Repeated explanations thereof are omitted for reasons of conciseness only.
DETAILED DESCRIPTION
[0042] FIG. 1 shows the principle design of a high pressure freezing system 100 which can also be used according to the present disclosure. For further explanations, reference is made to the literature cited above for the “EM ICE” and “EM HPM100” systems from Leica Microsystems, e.g. to DE 10 2013 003 164 A1 which further describes details of the high pressure freezing system 100 and a specific embodiment thereof. Further reference is made to review articles such as Kaech & Ziegler (see above). Be it noted that the present disclosure is in no way bound to being used in such a specific system, as the general inventive concept of providing a baffle structure or element for a stream of a cryogenic refrigerant being generally usable in high pressure freezing systems.
[0043] A sample cartridge 200-500 which is described in non-inventive and inventive embodiments hereinbelow and which is designated with X in FIG. 1 for reasons of generality may be placed in a high pressure chamber 90 of the system 100 via a loading device 80. For the freezing process, the high pressure chamber 90 is closed by locking the loading device 80 with locking pins 82. The freezing process is achieved by the supply of liquid nitrogen under high pressure which is suddenly introduced into the space in which the cartridge 200-500 is received. For this purpose the system 100 is equipped with a refrigerant supply 70. In the refrigerant supply 70, liquid nitrogen is fed from a refrigerant storage tank 72 via a refrigerant pump 74 with a downstream non return valve 76 to a compressed, air operated pressure intensifier 78. Here the refrigerant is brought to the target pressure of e.g. 2,100 bar. As mentioned already, the present disclosure can also be used in connection with different high-pressure freezing systems and in such systems a refrigerant pump may not be needed.
[0044] The outlet of the pressure intensifier 78 is connected to the high-pressure chamber 90 by a supply line via a high-pressure valve 92. The high-pressure valve 92 is designed to abruptly feed the refrigerant under high pressure to the inside of the high-pressure chamber 90 when it is opened, so that the sample held in the cartridge 200-500 is high pressure frozen in a very short time. A pressure sensor 94 and a temperature sensor 96 may be used to monitor the freezing process. Via an outlet opening 98 (outlet with silencer) the high pressure chamber 90 can be depressurized to allow the cryopreserved sample to be removed together with the sample cartridge 200-500.
[0045] In addition, a supply arrangement 60 for a further liquid (“filling liquid”), e.g. an alcohol (in particular ethanol) may be provided whereby the filling liquid is supplied to the high-pressure chamber 90 from a container 62 via a pump/dosing system 64 with a non-return valve 66 downstream of the pump/dosing system 64.
[0046] Also such an arrangement may not be used in connection with embodiments of the present disclosure.
[0047] FIG. 2 shows a sample cartridge 200 according to a non-inventive embodiment which is held in the end piece 52 of a holder 50. The holder 50 may be used to introduce the sample cartridge 200 into a high-pressure freezing system 100 as illustrated in FIG. 1 and may be part of, or used in, the loading device 80. Further details as to this specific aspect are also illustrated in DE 10 2019 003 164 A1. The end piece 52 is made, for example, of stainless steel; it is cup-shaped (with the cup oriented parallel to the longitudinal axis, thus horizontal in the design example) and has, in this specific embodiment, an optional recess 54 on the side corresponding to the location of a viewing window not shown. The end piece 52 includes two opposite parts 56 (also made of stainless steel) with a planoconcave shape. The parts 56 serve as spring tongues and provide a spring action to hold the parts of the sample cartridge together by engaging into grooves of the cartridge 200. The parts 56 are laterally offset for reasons of space requirements for the viewing window; in other designs, these elements may be attached vertically. An outlet 58 explained in connection with FIG. 3 below is also provided.
[0048] FIG. 3 shows the sample cartridge 200 not corresponding to the present disclosure already shown in FIG. 2 in an exploded view. The sample cartridge 200 comprises two holding elements 202, 204, each essentially of a half cylindrical shape. A middle plate 206 is held between the holding elements 202, 204 of the cartridge 200. The holding element 204 is shown in transparent representation in FIG. 2 before, which improves the view of the middle plate 206. The holding elements 202, 204 which each have a substantially semi-cylindrical basic shape, form a refrigerant channel 208 therebetween, the refrigerant channel 208 being formed by grooves along the longitudinal direction on the inner flat surfaces of the holding elements 202, 204. Through the refrigerant channel 208 of a refrigerant channel arrangement, a cryogenic refrigerant can be passed to a sample received in the middle plate 206. In this way, the refrigerant can approach to the sample from both sides, which promotes rapid cooling. In the design shown, a channel 208 is provided from each end face to further improve the supply of refrigerant. This allows, for high pressure cryofixation, the refrigerant to flow through the cartridge 200, essentially along the longitudinal axis of the cartridge 200, in the direction of the dotted arrow 210 in FIG. 3. An opening is provided in the holder 50 shown in FIG. 2 at a suitable position (not shown) which leads to the outlet 58 already mentioned, the latter communicating with the outlet opening 98 of the device 100 (see FIG. 1). In this configuration, gases or liquids displaced by the refrigerant (including any filling liquid such as ethanol) may be discharged. By proper dimensioning, a certain amount of cryogenic refrigerant may flow through the cartridge 200 without causing a pressure reduction within the time required for cryopreservation, and thereafter the discharge of the chamber filling will automatically cause a pressure release.
[0049] The holding elements 202 and 204 of the cartridge 200 may comprise lateral grooves or notches 212, e.g., formed in pairs opposite each other, for manipulation of the operational cartridge or fixing the components together. The elements of the cartridge 200 may be made for example of PEEK (polyether ether ketone) or of a transparent plastic (e.g. PC, poly carbonate). The middle plate 206 may be made of PEEK (polyether ether ketone), for example, which makes processing easier. Further details are also known from the prior art.
[0050] FIG. 4 shows the middle plate 206 which is used in the non-inventive cartridge 200 as shown in FIG. 3 and may also be used in the inventive cartridge 300, 400, or 500 as explained below, with geometrical adaptations as necessary, in a longitudinal section along a vertical plane, so that a space for holding a sample 10 is shown cut open. The middle plate 206 has a notch 214 on each side, which corresponds to the notches 212 mentioned of the holding elements 202, 204, and has a circular opening 216 in its center, in which the sample 10 is held between two enclosing elements 218, e.g. circular discs made of sapphire or another suitable material. The enclosing elements 218 are fixed in the opening 216 and their distance is adjusted by fixing, gasketing and/or spacer means 220. The enclosing elements themselves may also comprise one or more recesses in which the sample 10 can reside when they are brought in contact thereby reducing or eliminating the need for spacers, as mentioned. The enclosing elements 218 also serve to protect the sample 10 since the rapid flow of refrigerant required for cooling would otherwise carry the sample away. Further details are described in the prior art. As mentioned, a rapid inflow of refrigerant, however, may damage the enclosing elements or slightly dislocate them in a way to cause sample leakage which are problems the present disclosure seeks to address.
[0051] The entire cartridge 200 is generally dimensioned in such a way that the high pressures (e.g. above 2,000 bar) required for high pressure freezing can be built up and maintained within a period of preferably 200-500 ms, whereby rapid freezing of sample 10 is achieved within this time interval.
[0052] In FIGS. 5 to 7, sample cartridges according to preferred embodiments of the present disclosure are shown in exploded views and are indicated with 300, 400 and 500. The sample cartridges 300, 400 and 500 comprise cartridge elements which are indicated with reference numerals incremented by 100, 200 or 300 vis-à-vis those explained for cartridge 200. Explanations given above for the corresponding elements also apply for the cartridge elements of the cartridges 300, 400 and 500 unless the subsequent explanations differ and/or are incompatible therewith.
[0053] In FIGS. 5 to 7, the middle plates 306, 406 and 506 are displayed alongside the holding elements 302, 304, 402, 404 and 502, 504 in an exploded perspective view. In FIG. 6 and FIG. 7, the holding element 404, 504 is shown in a transparent way to make aspects of the embodiments of the disclosure visible. The middle plates 306, 406 and 506 are shown without a sample chamber being formed in their openings 316, 416 and 516, as this can be done essentially as described hereinbefore in connection with FIG. 2.
[0054] The sample cartridges 300, 400 and 500 according to FIGS. 5, 6 and 7 each comprise a baffle structure in the form of a circular and rectangular disc-like baffle element 350, 450 and 550 which will be described in more detail below. The baffle elements 350, 450 and 550 comprise engaging structures 351, 451, 551 for fixedly holding them at a position referred to as a “baffle position” hereinbefore. In the sample cartridge 300, the holding structure 351 is adapted to engage with a matching recess 353 formed in the middle plate 306. In the sample cartridges 400 and 500, two rod-like holding structures 451, 551 are provided which are adapted to engage in corresponding holes 452, 552 in the holding element 404, 504.
[0055] In the sample cartridge 300 according to FIG. 5, the baffle position corresponds to a position at the surface of the cartridge 300 from which a refrigerant channel 308 of the refrigerant channel arrangement extends to the sample chamber position which is indicated with reference numeral 320. As the refrigerant channel 308 would thus be blocked by the baffle structure 350, refrigerant feeding channels 352 are provided as a part of the refrigerant channel arrangement. They extend from a further position at the surface of the cartridge 300 to the (blocked) refrigerant channel 308 and thus allow an inflow of refrigerant thereto. A stream of refrigerant thus formed will impinge onto the sample chamber at the sample chamber position less violently and thus the risk of breakage is significantly reduced.
[0056] In order to position the baffle structure 350 in the cartridge 300, a recess 354 is provided in the holding elements 302, 304, the recess 354 being adapted to receive the baffle structure 350. The baffle structure comprises, as mentioned, a protrusion referred to as engaging structure 351 with a form to engage with a matching recess 353 in the middle plate 306 so that the baffle structure 350 is held firmly in place when the cartridge 300 is in its operational configuration.
[0057] In FIG. 6, only a limited number of elements are indicated with reference numerals as compared to the preceding illustrations. As shown, the sample cartridge 400 according to FIG. 6 comprises several refrigerant channels 408, 462, 464, 466, 468 which are provided to form the refrigerant channel arrangement, wherein the refrigerant channels 462, 464 are separated from the refrigerant channel 408 by the baffle structure 450. Thus, the refrigerant channel arrangement comprises a section extending from the surface of the cartridge 400 to the baffle position and a section from the baffle position to the sample chamber position 420, the sections being subdivided by the baffle structure 450.
[0058] In the cartridge 400 according to FIG. 6, the holding elements 402, 404 comprise recesses 456 to hold the baffle structure 450 and the middle plate 406 comprises a bore 458 through which the baffle structure 450 is adapted to extend when received in the recesses 456 of the holding elements 402, 404 and in the operational configuration of the cartridge elements 402, 404 and 406. The baffle structure can be fixedly mounted to one of the holding elements (shown for holding element 404 in FIG. 6), e.g. with suitable bolts or screws as the engagement elements 451 mentioned, which may permanently or removably engage with the holes 453. In the baffle structure 450, which has a rectangular shape, holes 460 are provided which are arranged at positions not coinciding with a central axis of the cartridge 400.
[0059] In FIG. 7, only a limited number, or an alternative set of, elements is indicated with reference numerals as compared to the preceding illustrations. As shown, the sample cartridge 500 according to FIG. 7 also comprises several refrigerant channels 508, 562, 564, 566 and 569, albeit in a refrigerant channel arrangement different from that of sample cartridge 400 shown in FIG. 6. Furthermore, the channel arrangement may at least in part be formed in the holding element 502 only. The refrigerant channel 569 is designed, in the embodiment shown, as a circular channel around the sample region 520 and comprises openings 570 towards the sample chamber position 520, thus forming a crown-like structure. The openings 570, in the embodiment shown, are distributed evenly around the circumference of the channel 569 but may, in other configurations, also e.g. have preferentially openings in a downstream region while no or less openings are provided in an upstream region. The channels 566 and 564 may again be separated from the channel 508 by the baffle structure 550. Thus, the refrigerant channel arrangement also comprises a section extending from the surface of the cartridge 500 to the baffle position and a section from the baffle position to the sample chamber position 520, the sections being subdivided by the baffle structure 450. The channel 569 is connected to the single downstream channel 566.
[0060] The cartridge 500 may be used with or without a separate disc-like baffle structure 550. If a separate baffle structure 550 is provided, this may be arranged as described for the previous figures or differently. If operated without a separate disc-like baffle structure 550, a wall part 571 between the channels 562 and 564 may serve as a baffle structure instead.
