The present invention relates to the technical fields of genetic engineering and bioinformatics, in particular, to a method for creating a new gene in an organism in the absence of an artificial DNA template, and a use thereof. The method comprises simultaneously generating DNA breaks at two or more different specific sites in the organism's genome, wherein the specific sites are genomic sites capable of separating different gene elements or different protein domains, and the DNA breaks are ligated to each other through non-homologous end joining (NHEJ) or homologous repair to generate a new combination of the different gene elements or different protein domains that is different from the original genome sequence, thereby creating a new gene. The new gene of the invention can change the growth, development, resistance, yield and other traits of the organism, and has great value in application.

The present invention relates to the technical fields of genetic engineering and bioinformatics, in particular, to a method for creating a new gene in an organism in the absence of an artificial DNA template, and a use thereof. The method comprises simultaneously generating DNA breaks at two or more different specific sites in the organism's genome, wherein the specific sites are genomic sites capable of separating different gene elements or different protein domains, and the DNA breaks are ligated to each other through non-homologous end joining (NHEJ) or homologous repair to generate a new combination of the different gene elements or different protein domains that is different from the original genome sequence, thereby creating a new gene. The new gene of the invention can change the growth, development, resistance, yield and other traits of the organism, and has great value in application.

In order to provide tobacco resistant to a virus, tobacco in accordance with the present invention is arranged such that: (i) a translation initiation factor eIF(iso)4E protein which is non-functional with respect to a virus is produced or (ii) expression of a translation initiation factor eIF(iso)4E gene is suppressed; and (a) a translation initiation factor eIF4E2 protein, which is non-functional with respect to a virus, is produced or (b) expression of a translation initiation factor eIF4E2 gene is suppressed.

The invention provides seed and plants of melon line HAR-DV15-4299MO. The invention thus relates to the plants, seeds, and tissue cultures of melon line HAR-DV15-4299MO and to methods for producing a melon plant produced by crossing such plants with themselves or with another melon plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of melon line HAR-DV15-4299MO comprising introduced beneficial or desirable traits.

Disclosed implementations for improving crop yield in arid environments. Implementations include a method comprising selecting a cover crop adapted to absorb one or more of atmospheric or soil moisture for storage in an underground portion of the cover crop, and planting the selected cover crop in a particular tract of land at a first time instance. The method additionally includes controllably terminating growth of the cover crop at a second time instance, subsequent to the first time instance, to initiate a decay phase of the cover crop to cause transfer of the moisture absorbed in the underground portion of the cover crop to soil of the tract of land, and planting a primary crop in the tract of land at a third time instance, the primary crop absorbing from the soil at least some of the moisture released during the decay phase of the cover crop.

The invention provides seeds and plants of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521 comprising introduced beneficial or desirable traits.

The invention provides seeds and plants of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato hybrid DRTH1025, tomato line FIR-XM19-4520, and tomato line FIR-XM19-4521 comprising introduced beneficial or desirable traits.

A Pentas plant having a monogenic, incompletely dominant doubled-flower gene, a method for breeding the same, and a method for providing a Pentas variety with a doubled-flower or semi-double flower phenotype are provided for the purpose of creating a doubled-flower or semi-double flower Pentas plant with voluminous florets and a high ornamental value.

A novel maize variety designated X03R607 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X03R607 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X03R607 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X03R607, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X03R607 are provided. Methods for producing maize varieties derived from maize variety X03R607 and methods of using maize variety X03R607 are disclosed.

A novel maize variety designated X03R607 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X03R607 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X03R607 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X03R607, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X03R607 are provided. Methods for producing maize varieties derived from maize variety X03R607 and methods of using maize variety X03R607 are disclosed.