This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

Devices that includes a first portion, the first portion including at least one fluid channel; a fluid actuator; an analysis sensor disposed within the fluid channel; a conductivity sensor disposed within the fluid channel; and an introducer; a second portion, the second portion comprising: at least one well, the well containing at least one material, wherein one of the first or second portion is moveable with respect to the other, wherein the introducer is configured to obtain at least a portion of the material from the at least one well and deliver it to the fluid channel, and wherein the fluid actuator is configured to move at least a portion of the material in the fluid channel.

A system and method of fluid delivery for providing a surface fluid pattern, the system comprising: a fluid delivery head for fluid flow therethrough, the fluid delivery head comprising: a fluid delivery surface having surface openings defined therein and arranged across the fluid delivery surface as a two-dimensional display; wherein at least some of the surface openings are grouped as a surface opening unit having at least one aspiration opening through which fluid can be provided to the fluid delivery surface and at least one injection opening through which fluid can be moved away from the fluid delivery surface, the surface opening unit comprising at least three surface openings positioned as a two-dimensional display and outwardly of at least one other surface opening.

A microfluidic system can include a substrate comprising an elastic material and defining a microfluidic channel. The substrate can have a first set of dimensions defining a thickness of a wall of the microfluidic channel and a second set of dimensions defining a width of the microfluidic channel. A transducer can be mechanically coupled with the substrate. The transducer can be operated at a predetermined frequency different from a primary thickness resonant frequency of the transducer. A thickness and a width of the transducer can be selected based on the first set of dimensions defining the thickness of the wall of the microfluidic channel and the second set of dimensions defining the width of the microfluidic channel.

A flow cell with a predetermined breaking barrier in a duct region of the flow cell. The flow cell has a cut-out in a substrate of the flow cell, which forms a portion of the duct region and has an opening in a surface of the substrate. The opening is hermetically sealed by a barrier film fused and/or glued to the surface and forming the predetermined breaking barrier. A layer is provided to be arranged over the barrier film. The layer can be stretched into the cut-out on breaking of the barrier film and formation of an access to a duct region section bounded by the barrier film and the layer.

Laboratory on chip and its layered manufacturing method, wherein the method includes: designing, by means of a computer program, a printed circuit (7), mixing and reaction cavities (3) of fluids, microchannels (2) and spaces (15) for the placement of electronic components to be found in each layer, mechanizing in one or more biocompatible substrates the different voids and passages that will make up the mixing and reaction cavities (3), microchannels (2), holes (8) that join the microchannels and spaces for the subsequent placement of electronic components (15), metallizing with a biocompatible conductive material those surfaces in which the printed circuit will be integrated (7) according to the design performed in the first step, generating the printed circuit (7) by photolithography and acid attack, bonding the electronic components in the corresponding spaces (15), joining all the layers that make up the final laboratory.

A method for separating motile organisms from other organisms. The method comprises controlling a fluid delivery unit to provide a fluid flow to a sample separating device (302). The fluid flow has a sample introduction flow velocity set so that a sample may be introduced into a sample introduction zone of the device. The sample introduction flow velocity is sufficiently high such that an organism in the sample is unable to exit the sample introduction zone. The method comprises controlling the fluid delivery unit to reduce the fluid flow velocity from the sample introduction flow velocity to an operational flow velocity lower than the sample introduction flow velocity (303). The operational flow velocity is selected such that motile organisms in the sample are able to swim against the fluid flow and enter a sample collection zone of the device.

(57) Abstract: The present invention relates to a container (1) for small liquid volumes having at least one inlet chamber (2), at least one outlet chamber (3), an open top end (4) and a bottom end (5), wherein the bottom end (5) comprises at least one access region (7); wherein the at least one inlet chamber (2) is capable of accommodating a swab stik (16); wherein the at least one inlet chamber (2) has at least one open top end (11a) and is connectable to at least a first free end (8) of a flowpath (9) via the at least one access region (7); and wherein the at least one outlet chamber (3) has at least one open top end (11b) and is connectable to at least another free end (10) of the flowpath (9) via the at least one access region (7); a kit, an interface for fluid handling, and a method for analyzing a sample liquid (18).

A microfluidic device for cell culture and screening, including a covering element with a plurality of openings configured for introducing and collecting fluids, and a central through hole; an intermediate element with a plurality of microchannels, a plurality of supply tanks and at least one waste tank, and a blind bottom cavity; a lower element, with a collecting tank and a recessed central portion; and a slide housed in a housing pocket. The intermediate element is interposed between the covering element and the lower element to form an upper optical window and at least one culture chamber. The plurality of microchannels puts in fluid communication the plurality of supply tanks, the at least one culture chamber and the waste tank.

A method of determining concentration of an analyte in a body fluid uses a mobile device having a camera and an ambient light detector. The ambient light detector has an ambient light sensor and/or an additional camera. An ambient light check is performed. In the ambient light check, ambient light is used to determine an item of ambient light information. If a validity criterion is determined to be met, the concentration of the analyte is determined by evaluating an image of at least a part of a reagent test region of an optical test strip having a sample of body fluid applied thereto. The image is captured by the camera. A mobile device, a kit, a computer program and a computer-readable storage medium are also disclosed.