A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

A measuring device for determining a gas concentration includes a gas-sensitive element, a sensing device, a stimulation unit, and a processing unit. The gas-sensitive element is configured to absorb a gas. The sensing device is configured to determine a parameter of the gas-sensitive element in a predetermined time period, where the parameter depends on an absorbed quantity of the gas. The stimulation unit is configured to stimulate the gas-sensitive element and accelerate desorption of the gas out of the gas-sensitive element. The processing unit is configured to determine a rate of change of the parameter, to control the stimulation such that a concentration of the gas in the gas-sensitive element lies outside of an equilibrium state, and to determine the gas concentration based on the rate of change.

A measuring device for determining a gas concentration includes a gas-sensitive element, a sensing device, a stimulation unit, and a processing unit. The gas-sensitive element is configured to absorb a gas. The sensing device is configured to determine a parameter of the gas-sensitive element in a predetermined time period, where the parameter depends on an absorbed quantity of the gas. The stimulation unit is configured to stimulate the gas-sensitive element and accelerate desorption of the gas out of the gas-sensitive element. The processing unit is configured to determine a rate of change of the parameter, to control the stimulation such that a concentration of the gas in the gas-sensitive element lies outside of an equilibrium state, and to determine the gas concentration based on the rate of change.

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

An inertion funnel includes a hollow ring with an attachment point on an outside edge and a plurality of output apertures on the inside edge allows for an even flow of gas around the circumference of the ring and through the output apertures into a filter funnel below. The attachment point allows the attachment of a hose or other fitting through which gas flows into the hollow ring, which rests above and around the opening of the filter funnel. On the inside of the ring, an angled downward opening allows gas to flow out of the ring and into the filter funnel. Uniformly distributed around the opening are vanes that direct the flow of gas from the ring into the filter funnel below.

An inertion funnel includes a hollow ring with an attachment point on an outside edge and a plurality of output apertures on the inside edge allows for an even flow of gas around the circumference of the ring and through the output apertures into a filter funnel below. The attachment point allows the attachment of a hose or other fitting through which gas flows into the hollow ring, which rests above and around the opening of the filter funnel. On the inside of the ring, an angled downward opening allows gas to flow out of the ring and into the filter funnel. Uniformly distributed around the opening are vanes that direct the flow of gas from the ring into the filter funnel below.

A gas fixture safety cover comprising: a first wall; a second wall attached to the first wall, the second wall is generally perpendicular to the first wall; a third wall attached to the second wall and generally perpendicular to the second wall; a fourth wall attached to the third wall and first wall, the fourth wall generally perpendicular to the third wall and first wall; a first nozzle vertical slot in the first wall and extending to the bottom of the first wall; a second nozzle vertical slot in the second wall and extending to the bottom of the second wall and where the height of the first handle vertical slot is higher than the height of the first nozzle vertical slot; where when the gas fixture safety cover can only slide down over a two nozzle and two handle gas fixture when the two handles are both turned off; where when the gas fixture safety cover has slid onto a gas fixture when the gas valve handle is turned off, the gas fixture safety cover abuts a counter top, and where when the gas fixture safety cover has slid onto a gas fixture when the gas valve handle is turned on, the gas fixture safety cover does not abut a counter top.

The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.