The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

There is provided a method of drying a biological material, comprising the steps of: a) generating a flow of microdroplets of the biological material having a dry matter content below 25% (weight/volume); b) contacting the microdroplets with a gas flow, wherein the ratio of the gas flow to the flow of microdroplets is at least 300.000:1, preferably at least 600.000:1, more preferably at least 800.000:1, thereby drying the biological material to form particles; c) separating the particles from the gas flow; d) drying the gas flow from step c); and e) recirculating the dried gas flow from step d) to step b). A corresponding apparatus is also provided.

There is provided a method of drying a biological material, comprising the steps of: a) generating a flow of microdroplets of the biological material having a dry matter content below 25% (weight/volume); b) contacting the microdroplets with a gas flow, wherein the ratio of the gas flow to the flow of microdroplets is at least 300.000:1, preferably at least 600.000:1, more preferably at least 800.000:1, thereby drying the biological material to form particles; c) separating the particles from the gas flow; d) drying the gas flow from step c); and e) recirculating the dried gas flow from step d) to step b). A corresponding apparatus is also provided.

A method for cryogenic cooling without fouling is disclosed. The method comprises providing a first cryogenic liquid saturated with a dissolved gas; expanding the first cryogenic liquid into a separation vessel, separating into a vapor, a second cryogenic liquid, and a first solid; drawing the vapor into a heat exchanger and the second cryogenic liquid and the first solid out of the separation vessel; cooling the vapor against a coolant through the heat exchanger, causing the vapor to form a third cryogenic liquid and a second solid, the second solid dissolving in the third cryogenic liquid; and combining the second cryogenic liquid and the first solid with the third cryogenic liquid, producing a final cooled slurry. In this manner, the cryogenic cooling is accomplished without fouling.

Filter assemblies and components therefor, are described. In an example arrangement, the crankcase ventilation filter assembly is configured to be serviced from either the top or the bottom. A rotational indexing arrangement is to ensure appropriate orientation of an internally received filter cartridge, and other components of the arrangement are provided. Methods of assembly, servicing and use are described.

A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles.

Filter assemblies and components therefor, are described. In an example arrangement, the filter assembly is configured to be serviced from either the top or the bottom. A rotational indexing arrangement is to ensure appropriate orientation of an internally received filter cartridge, and other components of the arrangement are provided. Methods of assembly, servicing and use are described.

The invention relates to fluid distribution in an exposure device. The exposure device, in some examples, is a device for exposing living cell cultures or cellular tissues to a fluid such as cigarette smoke, air and/or other gases or gas mixtures, for use in studies of the effects on the cell cultures or tissues of their exposure to the fluid. A fluid distribution member is described for distributing the fluid in the exposure device, the member including a plurality of apertures in a surface arranged such that, when the distribution member is fitted to the fluid exposure device, the distribution member receives fluid applied to the surface of the member and distributes the fluid, via the apertures, to samples to be exposed to the fluid in the exposure device.

A cartridge for dispensing a fluid is presented. The cartridge comprises a reservoir chamber for receiving the fluid and for receiving a ventilation gas. The reservoir chamber comprises an inlet for receiving the ventilation gas and an outlet for dispensing the fluid. At least a portion of the reservoir chamber is filled with the ventilation gas when in an operating position. The inlet is located in the portion being filled with the ventilation gas. The fluid comprises a reagent. The cartridge further comprises a baffle for restricting gas diffusion through the inlet. The reservoir chamber receives the ventilation gas via the baffle. The inlet is maintains a constant gas pressure within the portion of the reservoir chamber that is being filled with the ventilation gas.

A cartridge for dispensing a fluid is presented. The cartridge comprises a reservoir chamber for receiving the fluid and for receiving a ventilation gas. The reservoir chamber comprises an inlet for receiving the ventilation gas and an outlet for dispensing the fluid. At least a portion of the reservoir chamber is filled with the ventilation gas when in an operating position. The inlet is located in the portion being filled with the ventilation gas. The fluid comprises a reagent. The cartridge further comprises a baffle for restricting gas diffusion through the inlet. The reservoir chamber receives the ventilation gas via the baffle. The inlet is maintains a constant gas pressure within the portion of the reservoir chamber that is being filled with the ventilation gas.