Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

A power supply device includes a power conversion circuit configured to convert an input voltage into an output voltage, a controller, and an output switch. The controller is coupled to the power conversion circuit and configured to control the power conversion circuit to generate the output voltage for causing electrical coalescence of a multi-phase liquid mixture when the output voltage is applied to the multi-phase liquid mixture. The output switch is coupled between an output of the power conversion circuit and a terminal of the power supply device. The output switch is switchable among a first position at which the output of the power conversion circuit is coupled to the terminal, a second position at which the output of the power conversion circuit is grounded, and a third position at which the output of the power conversion circuit is electrically isolated from the terminal and the ground.

A power supply device includes a power conversion circuit configured to convert an input voltage into an output voltage, a controller, and an output switch. The controller is coupled to the power conversion circuit and configured to control the power conversion circuit to generate the output voltage for causing electrical coalescence of a multi-phase liquid mixture when the output voltage is applied to the multi-phase liquid mixture. The output switch is coupled between an output of the power conversion circuit and a terminal of the power supply device. The output switch is switchable among a first position at which the output of the power conversion circuit is coupled to the terminal, a second position at which the output of the power conversion circuit is grounded, and a third position at which the output of the power conversion circuit is electrically isolated from the terminal and the ground.

Electro-coalescer systems herein may include a vessel, a base plate separating a process chamber and an electric enclosure, rod-shaped ceramic insulated electrodes, and a sealing assembly. An end of the electrodes is located within the electric enclosure. The electrodes traverse respective through-holes of the base plate into or through the process chamber, where a second portion is supported by a spacer, configured to maintain a position of the electrodes while allowing fluid passage. The sealing assembly forms a seal between the through-holes and the rod-shaped insulated electrodes, preventing fluid traversing from the process chamber into the electric enclosure. The sealing assembly may include: a metal fitting disposed around the rod-shaped insulated electrode; metal o-rings; metal seats; and a closing nut. The metal fitting has a coefficient of thermal expansion similar to that of the ceramic insulator, thereby preventing breakage of the electrodes during use.

Electro-coalescer systems herein may include a vessel, a base plate separating a process chamber and an electric enclosure, rod-shaped ceramic insulated electrodes, and a sealing assembly. An end of the electrodes is located within the electric enclosure. The electrodes traverse respective through-holes of the base plate into or through the process chamber, where a second portion is supported by a spacer, configured to maintain a position of the electrodes while allowing fluid passage. The sealing assembly forms a seal between the through-holes and the rod-shaped insulated electrodes, preventing fluid traversing from the process chamber into the electric enclosure. The sealing assembly may include: a metal fitting disposed around the rod-shaped insulated electrode; metal o-rings; metal seats; and a closing nut. The metal fitting has a coefficient of thermal expansion similar to that of the ceramic insulator, thereby preventing breakage of the electrodes during use.

The present invention relates to a system for the separation of oil/water emulsions by electrocoalescence having a fluid conduction means or tubing, at least one cathode, at least one electrode, at least one anode, at least one power source and at least one spark gap for a cathode and a spark gap for the anode. Furthermore, the present invention also relates to a method for the separation of oil/water emulsions by electrocoalescence carried out by a system according to the invention.

The present invention relates to a system for the separation of oil/water emulsions by electrocoalescence having a fluid conduction means or tubing, at least one cathode, at least one electrode, at least one anode, at least one power source and at least one spark gap for a cathode and a spark gap for the anode. Furthermore, the present invention also relates to a method for the separation of oil/water emulsions by electrocoalescence carried out by a system according to the invention.

A coalescence method and related system are disclosed herein. A multiphase dispersion feed comprising first and second liquids (i.e. where droplets of the first liquid (dispersed phase) are dispersed in the second liquid (continuous phase)) is passed through a static mechanical droplet-coalescer comprising a channel characterized by a plurality of in-series segments, each segment characterized by a segment-specific-characteristic obstacle size and having geometric features disclosed herein. In embodiments of the invention, the static mechanical droplet-coalescer promotes coalescence between droplets of first liquid to form larger droplets of first liquid. Subsequently, after the dispersion exits the coalescer, the larger droplets are easier to remove from the second liquid (continuous phase) than the smaller droplets that coalesced into the larger droplets.

A coalescence method and related system are disclosed herein. A multiphase dispersion feed comprising first and second liquids (i.e. where droplets of the first liquid (dispersed phase) are dispersed in the second liquid (continuous phase)) is passed through a static mechanical droplet-coalescer comprising a channel characterized by a plurality of in-series segments, each segment characterized by a segment-specific-characteristic obstacle size and having geometric features disclosed herein. In embodiments of the invention, the static mechanical droplet-coalescer promotes coalescence between droplets of first liquid to form larger droplets of first liquid. Subsequently, after the dispersion exits the coalescer, the larger droplets are easier to remove from the second liquid (continuous phase) than the smaller droplets that coalesced into the larger droplets.