The present invention provides tissue equivalent scaffold structures and methods of production thereof. Such methods include providing a casting chamber comprising an elongate mould portion, axially disposing a lumen template within the elongate mould portion, and at least partly filling the casting chamber with a gel casting material comprising a matrix of fibrils or fibres and an interstitial fluid phase, such that a portion of the lumen template extends above the casting material. The fluid phase of the gel is allow to flow axially out of the elongate mould portion, in a restricted manner, thereby resulting in axial densification of the gel casting material to form a tissue equivalent tubular scaffold. Tissue equivalent scaffold structures according to the present invention are able to support cell populations both within the walls and on the surface of the construct. They have enhanced mechanical strength due to increased collagen density, and are customisable in terms of luminal diameter and wall thickness. They may find application in tubular tissue engineering.

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

The present disclosure relates to systems and methods for producing an extruded protein product. In particular, a system for making an extruded protein product using a system that includes a die including channel having a transverse cross section that is a continuous loop along at least a portion of the length of the die is disclosed.

Methods of tissue engineering, and more particularly methods and compositions for generating various vascularized 3D tissues, such as 3D vascularized embryoid bodies and organoids are described. Certain embodiments relate to a method of generating functional human tissue, the method comprising embedding an embryoid body or organoid in a tissue construct comprising a first vascular network and a second vascular network, each vascular network comprising one or more interconnected vascular channels; exposing the embryoid body or organoid to one or more biological agents, a biological agent gradient, a pressure, and/or an oxygen tension gradient, thereby inducing angiogenesis of capillary vessels to and/or from the embryoid body or organoid; and vascularizing the embryoid body or organoid, the capillary vessels connecting the first vascular network to the second vascular network, thereby creating a single vascular network and a perfusable tissue structure.

The present disclosure relates to systems and methods for producing an extruded protein product. In particular, a system for making an extruded protein product using a system that includes a die including channel having a transverse cross section that is a continuous loop along at least a portion of the length of the die is disclosed.

The invention relates to a method and a device for the production of portions from at least one continuous strand of a food product, in particular for the production of meatballs. The machine comprises: an extrusion device which can deliver a continuous strand in a vertical direction, and a portioning device that includes at least one cutting system. The cutting system comprises cutting elements (30) which can be moved transversely to the vertical direction between an open position, in which the strand passes between the cutting elements, and a closed position for cutting the strand and forming portions of the food product. In addition, each cutting system comprises ejection means (5) for ejecting the forming portions downwards from the cutting elements. The ejection means include at least one lower channel (5, 105) that is fluidly connected to a compressed fluid such that compressed fluid can be injected into the lower channel when the lower channel is in a closed position. The lower channel is formed in a body of the corresponding cutting element.

A water-soluble film (WSF) system comprises at least one active material embedded/entrapped in the film so as to provide precise and desired release of the at least one active material therefrom. The at least one active material includes detergents, enzymes, softeners, perfumes, pesticides, fungicides, active ingredients, dyes, pigments, hazardous chemicals, active agents for cleaning laundry, dishes, floorings, walls, furniture, fluffs, pulp, and combinations thereof. Online and offline processes can be used for the manufacture of multi-layered WSFs with or without liners. The WSFs can be formed in desired shapes to selectively entrap interacting/non-interacting materials and combinations thereof. The process also provides options for the use of a wide range of raw materials, liners such as paper, film, foil, fabric, etc.

Bioink compositions comprising a plurality of aromatic peptides and a matrix-forming material, formulations and kits for preparing same, additive manufacturing (bioprinting) processes utilizing same and three-dimensional objects obtained thereby are provided.

A method for manufacturing a medical patch for the treatment of chronic vascular and diabetic ulcers comprises the steps of preparing a tubular support, spraying toward the axial lateral surface of said support at least two separate, simultaneous, converging jets of two nebulized solutions containing fibrinogen and thrombin, respectively, rotating the support and/or orienting the jets in such a way as to deposit on said support a layer of material (M) of predetermined size, and incubating the material (M) until the fibrin contained therein is polymerized.

This disclosure relates to a hydrogel comprising a crosslinked biomolecule, wherein the hydrogel comprises microscale structures. Also described is a hydrogel comprising an ordered array of semi-spherical microbumps, wherein the hydrogel is bacteria-repellent. Also described is a hierarchically-structured protein hydrogel that inhibits long term attachment of multidrug resistant Staphylococcus aureus up to 100 over a flat hydrogel. Methods of making and uses thereof are also disclosed herein.