Nicotine containing article for smokeless and oral usage comprising a wrapping material and a nicotine containing product, the nicotine containing product being arranged inside the wrapping material, wherein the wrapping material encloses the nicotine containing product from all sides and thereby forms the nicotine containing article in the shape of a pouch, and wherein the wrapping material is fluid permeable and thereby allows a fluid, preferably with nicotine absorbed from the nicotine containing product, to pass through it. According to the invention at least a part of the wrapping material also comprises cooling agent which causes a cooling effect when using the nicotine containing article, wherein the cooling agent is neutral in taste and/or odour.

The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.

The object of an embodiment of the present invention is to improve the quality of a tobacco product. The present invention relates to a tobacco plant in which a mutation is introduced in a genome, the mutation causing suppression of a function of a specific endogenous gene.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that positively regulates alkaloid biosynthesis, such as nicotine biosynthesis. In particular, the present disclosure provides methods for the inhibition of Nicotiana benthamiana auxin response factor 1 (NbTF1) to reduce alkaloid biosynthesis in plants.

A machine and method for packaging tobacco sachets, comprising a plurality of linearly arranged, multi-functional, vertically moveable chambers having fluid sealing means on lower portions thereof, conveyor means disposed below the plurality of multi-functional, vertically moveable chambers, and a lidding apparatus disposed downstream of and in communication with the plurality of vertically moveable chambers through the conveyor means.

A smokeless tobacco alternative kit having a container defining a strip cavity and having a lid, the container and lid operably configured to couple together to encapsulate the strip cavity and a plurality of strips disposed within the strip cavity. Each of the plurality of strips have a first end, a second end opposing the first end of the strip, and a strip length separating the first and second ends of the strip and have a non-woven and absorbent fabric material surrounding and coupled to an absorbent cotton material, wherein the non-woven and absorbent fabric material and the absorbent cotton material spanning the strip length and absorbed with an organic release liquid of a nicotine-free, tobacco-free, and gluten-free liquid operably configured to transport through the fabric material and cotton material when subjected to a compressive force.

A smokeless tobacco pastille configured for insertion into the mouth of a user is provided. The smokeless tobacco pastille can include (i) a tobacco material present in an amount of less than about 40 dry weight percent, based on the total dry weight of the pastille; (ii) at least one natural gum binder present in an amount of at least about 25 dry weight percent; and (iii) a plurality of sugar alcohols present in a total amount of at least about 40 dry weight percent, the predominant component of the plurality of sugar alcohols being isomalt or erythritol, wherein the shape of the smokeless tobacco pastille is moldable in the oral cavity.

A smokeless tobacco pastille configured for insertion into the mouth of a user is provided. The smokeless tobacco pastille can include (i) a tobacco material present in an amount of less than about 40 dry weight percent, based on the total dry weight of the pastille; (ii) at least one natural gum binder present in an amount of at least about 25 dry weight percent; and (iii) a plurality of sugar alcohols present in a total amount of at least about 40 dry weight percent, the predominant component of the plurality of sugar alcohols being isomalt or erythritol, wherein the shape of the smokeless tobacco pastille is moldable in the oral cavity.

This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.