The present invention relates to a top-down symmetric deconstruction approach which provides a novel alternative means to successfully identify a useful polypeptide “building block” for subsequent “bottom-up” de novo design of target protein architecture. The present invention also pertains to a novel peptides isolated by top-down symmetric deconstruction which may be useful for design or directed evolution of novel proteins with novel functionalities.

Isolated polypeptides with steroid binding activity and methods for their use as therapeutics and detection agents are disclosed herein.

According to an artificial bioparticle characterized in that a leucine zipper is integrated in each N terminal of an MVP constituting a waist of a vault and a method of manufacturing an artificial bioparticle in which a leucine zipper gene is integrated and expressed in a side to be an N terminal of an MVP gene, a novel artificial bioparticle including a vault of which large internal space can effectively be made use of, which can be used as a nanocapsule applicable to a drug delivery system (DDS), and a method of manufacturing the same are provided.

A monoclonal antibody to KIR2DS1 or a fragment containing an antigen-binding region thereof has VL having an amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR1, having an amino acid sequence of SEQ ID NO: 2 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR2, and having an amino acid sequence of SEQ ID NO: 3 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR3; and VH having an amino acid sequence of SEQ ID NO: 4 or SEQ ID NO: 5 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR1, having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR2, and having an amino acid sequence of any one selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 8, and SEQ ID NO: 9 or an amino acid sequence having the same amino acid sequence as the amino acid sequence except that one or several amino acids are conservatively substituted as CDR3.

A process for preparing sebacic acid by reacting in a first step (i) linoleic acid with water catalyzed by an oleate hydratase to form 10-hydroxy-12-octadecenoic acid, in a second step (ii) pyrolysing the 10-hydroxy-12-octadecenoic acid to 1-octene and 10-oxo-decanoic acid and in a third step (iii) oxidizing the 10-oxo-decanoic acid to sebacic acid.

Compositions containing a protease inhibitor for treating viral infections and methods for treating a patient before, during, or after exposure to an infectious agent by administering such compositions are described herein.

Compositions containing a protease inhibitor for treating viral infections and methods for treating a patient before, during, or after exposure to an infectious agent by administering such compositions are described herein.

Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Vibrio species such as cellular extracts from Vibrio natriegens.

Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Vibrio species such as cellular extracts from Vibrio natriegens.

The present invention is directed to a process for making a tissue regeneration matrix. The process comprises providing a collagen-tropoelastin dispersion; freeze-drying the dispersion to provide a porous freeze-dried matrix; and then crosslinking the porous freeze-dried matrix. The present invention is also directed to a tissue regeneration matrix prepared by the process.