The present disclosure relates to a natural dyeing method using shouliang yam rhizome, and more particularly to a natural dyeing method using shouliang yam rhizome, which prevents the discoloration of a naturally dyed fabric, is harmless to the human body, and exhibits excellent color development, excellent antibacterial activity, excellent deodorization, and excellent dyeing fastness such as friction fastness and light fastness.

The anthocyanin synthesized from date palm may include co-pigmented anthocyanin complexes synthesized by extracting an anthocyanin from date palm leaves and reacting the anthocyanin with phenolic compounds also resulting from date palm leaf extraction in order to produce a co-pigmented anthocyanin complex. Date palm leaf powder may be mixed with water containing about 1.08% hydrochloric acid in a glass or ceramic lined reactor, and extraction may be performed by boiling the mixture for an hour or more. The color of the co-pigmented anthocyanin complex produced by this method may be adjusted by continuing the heating in half hour increments, in order to deepen the hue of the co-pigmented anthocyanin complex. The co-pigmented anthocyanin complex may be separated from the liquid mixture by first filtering the liquid mixture, refining the liquid mixture by adding gelatin to form a colloidal solution, and freeze-drying the refined liquid mixture.

The anthocyanin synthesized from date palm may include co-pigmented anthocyanin complexes synthesized by extracting an anthocyanin from date palm leaves and reacting the anthocyanin with phenolic compounds also resulting from date palm leaf extraction in order to produce a co-pigmented anthocyanin complex. Date palm leaf powder may be mixed with water containing about 1.08% hydrochloric acid in a glass or ceramic lined reactor, and extraction may be performed by boiling the mixture for an hour or more. The color of the co-pigmented anthocyanin complex produced by this method may be adjusted by continuing the heating in half hour increments, in order to deepen the hue of the co-pigmented anthocyanin complex. The co-pigmented anthocyanin complex may be separated from the liquid mixture by first filtering the liquid mixture, refining the liquid mixture by adding gelatin to form a colloidal solution, and freeze-drying the refined liquid mixture.

The anthocyanin synthesized from date palm may include co-pigmented anthocyanin complexes synthesized by extracting an anthocyanin from date palm leaves and reacting the anthocyanin with phenolic compounds also resulting from date palm leaf extraction in order to produce a co-pigmented anthocyanin complex. Date palm leaf powder may be mixed with water containing about 1.08% hydrochloric acid in a glass or ceramic lined reactor, and extraction may be performed by boiling the mixture for an hour or more. The color of the co-pigmented anthocyanin complex produced by this method may be adjusted by continuing the heating in half hour increments, in order to deepen the hue of the co-pigmented anthocyanin complex. The co-pigmented anthocyanin complex may be separated from the liquid mixture by first filtering the liquid mixture, refining the liquid mixture by adding gelatin to form a colloidal solution, and freeze-drying the refined liquid mixture.

The anthocyanin synthesized from date palm may include co-pigmented anthocyanin complexes synthesized by extracting an anthocyanin from date palm leaves and reacting the anthocyanin with phenolic compounds also resulting from date palm leaf extraction in order to produce a co-pigmented anthocyanin complex. Date palm leaf powder may be mixed with water containing about 1.08% hydrochloric acid in a glass or ceramic lined reactor, and extraction may be performed by boiling the mixture for an hour or more. The color of the co-pigmented anthocyanin complex produced by this method may be adjusted by continuing the heating in half hour increments, in order to deepen the hue of the co-pigmented anthocyanin complex. The co-pigmented anthocyanin complex may be separated from the liquid mixture by first filtering the liquid mixture, refining the liquid mixture by adding gelatin to form a colloidal solution, and freeze-drying the refined liquid mixture.

Described are biocomposites comprising melanin. Uses of melanin biocomposites in methods of heat generation and radiation protection are also described. 3D printed filaments comprising melanin and methods and making and using thereof are further described.

The present invention employs the technique for expansion microscopy (E×M) utilizing anchorable derivatives of hematoxylin and eosin (H&E) stained tissue specimens within tissue samples, in order to achieve high resolution detection of biomolecules with precise morphological features of cells and nuclei, which in turn, allows for effective for accurate early stage disease diagnosis including various cancers. Staining with H&E employs anchorable derivatives that can be cross-linked into the E×M hydrogel matrix.

The present invention employs the technique for expansion microscopy (E×M) utilizing anchorable derivatives of hematoxylin and eosin (H&E) stained tissue specimens within tissue samples, in order to achieve high resolution detection of biomolecules with precise morphological features of cells and nuclei, which in turn, allows for effective for accurate early stage disease diagnosis including various cancers. Staining with H&E employs anchorable derivatives that can be cross-linked into the E×M hydrogel matrix.

The present application discloses methods for extracting melanin from a fungus, where the fungus is Inonotus obliquus (chaga), Fomes fomentarius, Ganoderma tsugae, or Phellinus igniarius. In the methods, a sample of the fungus is dried and pulverized to form a powder sample. The powder sample is refluxed in a sodium hydroxide solution to form a mixture. The mixture is separated into a liquid portion and a solid portion via centrifugation or vacuum filtration, resulting in the liquid portion including the melanin extract. The separated liquid portion including the melanin is acidified with a hydrochloric acid solution to precipitate the melanin. The precipitated melanin is then isolated via centrifugation or vacuum filtration such that the melanin collects as the pellet or precipitate. The melanin precipitate is then purified by washing with dilute HCl and deionized, distilled water. The purified melanin is then dried to produce the extracted fungal melanin.

The present application discloses methods for extracting melanin from a fungus, where the fungus is Inonotus obliquus (chaga), Fomes fomentarius, Ganoderma tsugae, or Phellinus igniarius. In the methods, a sample of the fungus is dried and pulverized to form a powder sample. The powder sample is refluxed in a sodium hydroxide solution to form a mixture. The mixture is separated into a liquid portion and a solid portion via centrifugation or vacuum filtration, resulting in the liquid portion including the melanin extract. The separated liquid portion including the melanin is acidified with a hydrochloric acid solution to precipitate the melanin. The precipitated melanin is then isolated via centrifugation or vacuum filtration such that the melanin collects as the pellet or precipitate. The melanin precipitate is then purified by washing with dilute HCl and deionized, distilled water. The purified melanin is then dried to produce the extracted fungal melanin.